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Bacillus thuringiensis

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https://www.readbyqxmd.com/read/28342673/therapeutic-use-of-bacillus-thuringiensis-in-the-treatment-of-psoroptic-mange-in-naturally-infested-new-zealand-rabbits
#1
Emmanuel Dunstand-Guzmán, Claudia Hallal-Calleros, Jorge Morales-Montor, Víctor Manuel Hernández-Velázquez, Juan José Zárate-Ramos, Kurt L Hoffman, Guadalupe Peña-Chora, Fernando Iván Flores-Pérez
Bacillus thuringiensis is a bacteria known for its bioinsecticidal toxins and it has been proposed as an alternative in the treatment of several parasites that infect domestic animals (helminths, ticks, mites). In this work, we evaluated the clinical efficiency of the Bacillus thuringiensis GP532 strain in the treatment of six rabbits naturally infested with the P. cuniculi mite. GP532 extract (10mg/ml) was applied by aspersion in both pinna, with a second application after seven days, and the therapeutic effect was measured in both qualitative and quantitative manner...
March 9, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28335365/antibacterial-properties-of-visible-light-responsive-carbon-containing-titanium-dioxide-photocatalytic-nanoparticles-against-anthrax
#2
Der-Shan Sun, Jyh-Hwa Kau, Hsin-Hsien Huang, Yao-Hsuan Tseng, Wen-Shiang Wu, Hsin-Hou Chang
The bactericidal activity of conventional titanium dioxide (TiO₂) photocatalyst is effective only on irradiation by ultraviolet light, which restricts the applications of TiO₂ for use in living environments. Recently, carbon-containing TiO₂ nanoparticles [TiO₂(C) NP] were found to be a visible-light-responsive photocatalyst (VLRP), which displayed significantly enhanced antibacterial properties under visible light illumination. However, whether TiO₂(C) NPs exert antibacterial properties against Bacillus anthracis remains elusive...
December 9, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334359/effectiveness-of-microbial-and-chemical-insecticides-for-supplemental-control-of-bollworm-on-bt-and-non-bt-cottons
#3
N S Little, R G Luttrell, K C Allen, O P Perera, K A Parys
Laboratory and field experiments were conducted to determine the effectiveness of microbial and chemical insecticides for supplemental control of bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), on non-Bt (DP1441RF) and Bt (DP1321B2RF) cottons. Neonate and 3rd instar larvae survival was evaluated on leaf tissue treated with microbial and chemical insecticides including a commercial formulation of Bacillus thuringiensis (Dipel), a Heliothis (Helicoverpa) nuclear polyhedrosis virus (NPV; Gemstar), λ-cyhalothrin (Karate Z), and chlorantraniliprole (Prevathon)...
March 18, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334341/large-scale-evaluation-of-association-between-pheromone-trap-captures-and-cotton-boll-infestation-for-pink-bollworm-lepidoptera-gelechiidae
#4
Yves Carrière, Larry Antilla, Leighton Liesner, Bruce E Tabashnik
Although transgenic cotton producing insecticidal proteins from Bacillus thuringiensis (Bt) is a cornerstone for pink bollworm control in some countries, integrated pest management remains important for bolstering sustainability of Bt cotton and is critical for controlling pink bollworm where Bt cotton is not available or where this pest has evolved resistance to Bt cotton. Here, we used data on moth captures in gossyplure-baited pheromone traps and boll infestations for 163 Bt and 152 non-Bt cotton fields from Arizona to evaluate accuracy of chemical control decisions relying on moth trapping data and capacity of Bt cotton to suppress survival of offspring produced by moths...
March 16, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334249/lethal-and-sublethal-effects-of-some-chemical-and-biological-insecticides-on-tuta-absoluta-lepidoptera-gelechiidae-eggs-and-neonates
#5
Z Nozad-Bonab, M J Hejazi, Sh Iranipour, M Arzanlou
Tuta absoluta (Meyrick) is one of the serious pests of tomatoes. This study was conducted to evaluate the effects of some chemical and biological insecticides on T. absoluta . The insecticides tested were diazinon, dichlorvos, chlorantraniliprole, deltamethrin, acetamiprid, imidacloprid, spinosad, abamectin, indoxacarb, Bacillus thuringiensis Berliner, Beauveria bassiana (Balsamo) Vuillemin, and Metarhizium anisopliae (Metschnikoff) Sorokin. Sublethal effects for the most effective insecticides tested, namely, abamectin, spinosad, chlorantraniliprole, and indoxacarb were assessed, and life table parameters were calculated...
March 13, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28330297/differential-expression-and-characterization-of-cypermethrin-degrading-potential-proteins-in-bacillus-thuringiensis-strain-sg4
#6
Pankaj, Geeta Negi, Saurabh Gangola, Priyanka Khati, Govind Kumar, Anjana Srivastava, Anita Sharma
A cypermethrin-degrading bacterium (SG4) was isolated from the pesticide-contaminated soil in the agricultural field of the crop research centre of the University, and characterized as Bacillus thuringiensis strain, SG4. The bacterium degraded 78.9 % of cypermethrin (50 ppm) in 15 days when grown in a minimal medium. To understand the functional proteins of cypermethrin degradation in Bacillus thuringiensis strain SG4, a comparative proteomic analysis was performed in the presence/absence of cypermethrin after 5 days of incubation in minimal medium...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330277/classical-and-bayesian-predictions-applied-to-bacillus-toxin-production
#7
Karim Ennouri, Rayda Ben Ayed, Maura Mazzarello, Ennio Ottaviani, Fathi Hertelli, Hichem Azzouz
Bacillus thuringiensis is a bacterium with unusual properties that make it useful for pest control in ecoagriculture. It can form a parasporal crystal containing polypeptides (also called delta-endotoxins). These entomopathogenic toxins are made during the stationary phase of the bacterial growth cycle and were initially characterized as an insect pathogen. Nowadays, the use of saturated two-level designs is very popular. This method is especially used in industrial applications where the cost of experiments is expensive...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330130/isolation-and-characterization-of-bacterial-endophytes-of-curcuma-longa-l
#8
Ajay Kumar, Ritu Singh, Akhilesh Yadav, D D Giri, P K Singh, Kapil D Pandey
Fourteen endophytic bacterial isolates were isolated from the rhizome of Curcuma longa L. were characterized on the basis of morphology, biochemical characteristics and 16S rRNA gene sequence analysis. The isolates were identified to six strains namely Bacillus cereus (ECL1), Bacillus thuringiensis (ECL2), Bacillus sp. (ECL3), Bacillus pumilis (ECL4), Pseudomonas putida (ECL5), and Clavibacter michiganensis (ECL6). All the strains produced IAA and solubilized phosphate and only two strains produced siderophore (ECL3 and ECL5) during plant growth promoting trait analysis...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330080/transgenic-rice-plants-expressing-synthetic-cry2ax1-gene-exhibits-resistance-to-rice-leaffolder-cnaphalocrosis-medinalis
#9
R Manikandan, N Balakrishnan, D Sudhakar, V Udayasuriyan
Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast transit peptide sequence was fused with synthetic cry2AX1 gene to target its protein in chloroplasts. Sixteen PCR positive lines of rice were generated by Agrobacterium mediated transformation using immature embryos...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327568/aminopeptidase-n1-is-involved-in-bacillus-thuringiensis-cry1ac-toxicity-in-the-beet-armyworm-spodoptera-exigua
#10
Lin Qiu, Songhe Cui, Lang Liu, Boyao Zhang, Weihua Ma, Xiaoping Wang, Chaoliang Lei, Lizhen Chen
Understanding how insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) interact with their hosts is crucial to fully explain the molecular bases of Bt specificity and insecticidal activity. Previous studies support ATP binding cassette transporters (ABCC2/3) and one cadherin-like protein are Cry1Ac functional receptors in the beet armyworm (Spodoptera exigua). In this study, a combined one-dimensional gel electrophoresis and immunoblotting approach identified aminopeptidase N (APNs) as putative Cry1Ac binding proteins in the midgut brush border membrane of S...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28321527/effects-of-site-mutations-within-the-22%C3%A2-kda-no-core-fragment-of-the-vip3aa11-insecticidal-toxin-of-bacillus-thuringiensis
#11
Ming Liu, Rongmei Liu, Guoxing Luo, Haitao Li, Jiguo Gao
Bacillus thuringiensis vegetative insecticidal proteins (VIPs) are not homologous to other known Cry proteins, and they act against lepidopteran larvae via a unique process. All reported studies on the mode of action of Vip3 proteins have been performed on the Vip3A family, mostly on the Vip3Aa subfamily. Vip3Aa proteins are activated by midgut proteases, and they cross the peritrophic membrane and bind specific proteins in apical membrane epithelial midgut cells, which results in pore formation and, eventually, death to the insects...
March 20, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28316900/influence-of-environmental-factors-on-biodegradation-of-quinalphos-by-bacillus-thuringiensis
#12
Venkata Subba Reddy Gangireddygari, Praveen Kumar Kalva, Khayalethu Ntushelo, Manjunatha Bangeppagari, Arnaud Djami Tchatchou, Rajasekhar Reddy Bontha
BACKGROUND: The extensive and intensive uses of organophosphorus insecticide-quinalphos in agriculture, pose a health hazard to animals, humans, and environment because of its persistence in the soil and crops. However, there is no much information available on the biodegradation of quinalphos by the soil micro-organisms, which play a significant role in detoxifying pesticides in the environment; so research is initiated in biodegradation of quinalphos. RESULTS: A soil bacterium strain, capable of utilizing quinalphos as its sole source of carbon and energy, was isolated from soil via the enrichment method on minimal salts medium (MSM)...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28316108/cry1a-b-16-toxin-from-bacillus-thuringiensis-theoretical-refinement-of-three-dimensional-structure-and-prediction-of-peptides-as-molecular-markers-for-detection-of-genetically-modified-organisms
#13
Alexandra Plácido, Andreia Coelho, Lucas Abreu do Nascimento, Andreanne Gomes Vasconcelos, Maria Fátima Barroso, Joilson Ramos-Jesus, Vladimir Costa, Francisco das Chagas Alves Lima, Cristina Delerue-Matos, Ricardo Martins Ramos, Mariela M Marani, José Roberto de Souza de Almeida Leite
Transgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, e.g., biosensors. In the absence of an available empirical structure of Cry1A(b)16 protein, a theoretical model was effectively generated, in this work, by homology modeling and molecular dynamics simulations based on two available homologous protein structures...
March 18, 2017: Proteins
https://www.readbyqxmd.com/read/28294483/the-detection-and-quantification-of-bacillus-thuringiensis-spores-from-soil-and-swabs-using-quantitative-pcr-as-a-model-system-for-routine-diagnostics-of-bacillus-anthracis
#14
Vera Sedlackova, Radka Dziedzinska, Vladimir Babak, Petr Kralik
AIMS: To optimize the DNA isolation for the routine detection and quantification of bacillary spores in soil and swabs. The procedure is primarily intended for diagnostics of Bacillus anthracis spores, but due to its high pathogenicity B. thuringiensis served as its surrogate organism. METHODS AND RESULTS: Various commercial kits for soils and swabs in combination with quantitative PCR were tested with different results. The PowerSoil DNA kit and the Ultra Clean Microbial DNA kit gave the best results for the extraction from soil and swabs, respectively...
March 10, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28292241/microbial-pest-control-agents-are-they-a-specific-and-safe-tool-for-insect-pest-management
#15
Caroline Deshayes, Myriam Siegwart, David Pauron, Josy-Anne Froger, Bruno Lapied, Véronique Apaire-Marchais
Microorganisms (viruses, bacteria and fungi) or their bioactive agents can be used as active substances and therefore are referred as Microbial Pest Control Agents (MPCA). They are used as alternative strategies to chemical insecticides to counteract the development of resistances and to reduce adverse effects on both environment and human health. These natural entomopathogenic agents, which have specific modes of action, are generally considered safer as compared to conventional chemical insecticides. Baculoviruses are the only viruses being used as the safest biological control agents...
March 14, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28282927/in-vivo-crystallization-of-three-domain-cry-toxins
#16
REVIEW
Rooma Adalat, Faiza Saleem, Neil Crickmore, Shagufta Naz, Abdul Rauf Shakoori
Bacillus thuringiensis (Bt) is the most successful, environmentally-friendly, and intensively studied microbial insecticide. The major characteristic of Bt is the production of proteinaceous crystals containing toxins with specific activity against many pests including dipteran, lepidopteran, and coleopteran insects, as well as nematodes, protozoa, flukes, and mites. These crystals allow large quantities of the protein toxins to remain stable in the environment until ingested by a susceptible host. It has been previously established that 135 kDa Cry proteins have a crystallization domain at their C-terminal end...
March 9, 2017: Toxins
https://www.readbyqxmd.com/read/28280018/complete-genome-sequences-of-bacillus-phages-dirtybetty-and-kida
#17
Kelly Flounlacker, Rachel Miller, Diana Marquez, Allison Johnson
We report here the genome sequences of two bacteriophages of the Bacillus cereus group, DirtyBetty and Kida. These bacteriophages are double-stranded DNA-containing Myoviridae isolated from soil samples using Bacillus thuringiensis subsp. kurstaki as their host bacteria.
March 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28277618/the-within-host-dynamics-of-infection-in-trans-generationally-primed-flour-beetles
#18
Ann T Tate, Peter Andolfatto, Jeffery P Demuth, Andrea L Graham
Many taxa exhibit plastic immune responses initiated after primary microbial exposure that provide increased protection against disease-induced mortality and the fitness costs of infection. In several arthropod species, this protection can even be passed from parents to offspring through a phenomenon called trans-generational immune priming. Here, we first demonstrate that trans-generational priming is a repeatable phenomenon in flour beetles (Tribolium castaneum) primed and infected with Bacillus thuringiensis (Bt)...
March 9, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28274717/rna-interference-knockdown-of-aminopeptidase-n-genes-decrease-the-susceptibility-of-chilo-suppressalis-larvae-to-cry1ab-cry1ac-and-cry1ca-expressing-transgenic-rice
#19
Lin Qiu, Jinxing Fan, Boyao Zhang, Lang Liu, Xiaoping Wang, Chaoliang Lei, Yongjun Lin, Weihua Ma
Transgenic rice expressing Bacillus thuringiensis (Bt) Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice in Asia. Understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. In this study, knockdown of two aminopeptidase N genes (APN1 and APN2) by RNA interference resulted in decreased susceptibility of C. suppressalis larvae to the Bt rice varieties TT51 (Cry1Ab and Cry1Ac fusion genes) and T1C-19 (Cry1Ca), but not T2A-1 (Cry2Aa)...
March 6, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28273400/fitness-of-bt-resistant-cabbage-loopers-on-bt-cotton-plants
#20
Guillaume Tetreau, Ran Wang, Ping Wang
Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt-crops in agriculture. The fitness of Bt-resistant insects on Bt- and non-Bt-plants is a key parameter that determines the development of Bt-resistance in insect populations. In this study, a comprehensive analysis of the fitness of Bt-resistant Trichoplusia ni strains on Bt-cotton leaves was conducted. The Bt-resistant T. ni strains carried two genetically independent mechanisms of resistance to Bt toxins Cry1Ac and Cry2Ab...
March 8, 2017: Plant Biotechnology Journal
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