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biodosimetry

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https://www.readbyqxmd.com/read/28220056/a-lipidomic-and-metabolomic-serum-signature-from-nonhuman-primates-exposed-to-ionizing-radiation
#1
Evan L Pannkuk, Evagelia C Laiakis, Tytus D Mak, Giuseppe Astarita, Simon Authier, Karen Wong, Albert J Fornace
INTRODUCTION: Due to dangers associated with potential accidents from nuclear energy and terrorist threats, there is a need for high-throughput biodosimetry to rapidly assess individual doses of radiation exposure. Lipidomics and metabolomics are becoming common tools for determining global signatures after disease or other physical insult and provide a "snapshot" of potential cellular damage. OBJECTIVES: The current study assesses changes in the nonhuman primate (NHP) serum lipidome and metabolome 7 days following exposure to ionizing radiation (IR)...
May 2016: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28211757/mice-and-the-a-bomb-irradiation-systems-for-realistic-exposure-scenarios
#2
Guy Garty, Yanping Xu, Carl Elliston, Stephen A Marino, Gerhard Randers-Pehrson, David J Brenner
Validation of biodosimetry assays is normally performed with acute exposures to uniform external photon fields. Realistically, exposure to a radiological dispersal device or reactor leak will include exposure to low dose rates and likely exposure to ingested radionuclides. An improvised nuclear device will likely include a significant neutron component in addition to a mixture of high- and low-dose-rate photons and ingested radionuclides. We present here several novel irradiation systems developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry to provide more realistic exposures for testing of novel biodosimetric assays...
February 17, 2017: Radiation Research
https://www.readbyqxmd.com/read/28140791/impact-of-neutron-exposure-on-global-gene-expression-in-a-human-peripheral-blood-model
#3
Constantinos G Broustas, Yanping Xu, Andrew D Harken, Mashkura Chowdhury, Guy Garty, Sally A Amundson
The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1-1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry...
January 31, 2017: Radiation Research
https://www.readbyqxmd.com/read/28117817/detection-of-inter-chromosomal-stable-aberrations-by-multiple-fluorescence-in-situ-hybridization-mfish-and-spectral-karyotyping-sky-in-irradiated-mice
#4
Rupak Pathak, Igor Koturbash, Martin Hauer-Jensen
Ionizing radiation (IR) induces numerous stable and unstable chromosomal aberrations. Unstable aberrations, where chromosome morphology is substantially compromised, can easily be identified by conventional chromosome staining techniques. However, detection of stable aberrations, which involve exchange or translocation of genetic materials without considerable modification in the chromosome morphology, requires sophisticated chromosome painting techniques that rely on in situ hybridization of fluorescently labeled DNA probes, a chromosome painting technique popularly known as fluorescence in situ hybridization (FISH)...
January 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28067089/metabolomic-applications-in-radiation-biodosimetry-exploring-radiation-effects-through-small-molecules
#5
Evan L Pannkuk, Albert J Fornace, Evagelia C Laiakis
PURPOSE: Exposure of the general population to ionizing radiation has increased in the past decades, primarily due to long distance travel and medical procedures. On the other hand, accidental exposures, nuclear accidents, and elevated threats of terrorism with the potential detonation of a radiological dispersal device or improvised nuclear device in a major city, all have led to increased needs for rapid biodosimetry and assessment of exposure to different radiation qualities and scenarios...
January 9, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27974505/metabolomic-screening-using-esi-ft-ms-identifies-potential-radiation-responsive-molecules-in-mouse-urine
#6
Daisuke Iizuka, Susumu Yoshioka, Hidehiko Kawai, Shunsuke Izumi, Fumio Suzuki, Kenji Kamiya
The demand for establishment of high-throughput biodosimetric methods is increasing. Our aim in this study was to identify low-molecular-weight urinary radiation-responsive molecules using electrospray ionization Fourier transform mass spectrometry (ESI-FT MS), and our final goal was to develop a sensitive biodosimetry technique that can be applied in the early triage of a radiation emergency medical system. We identified nine metabolites by statistical comparison of mouse urine before and 8 h after irradiation...
December 14, 2016: Journal of Radiation Research
https://www.readbyqxmd.com/read/27886989/u-s-department-of-defense-multiple-parameter-biodosimetry-network
#7
William F Blakely, Alexander Romanyukha, Selena M Hayes, Ricardo A Reyes, H Michael Stewart, Matthew H Hoefer, Anthony Williams, Thad Sharp, L Andrew Huff
The U.S. Department of Defense (USDOD) service members are at risk of exposure to ionizing radiation due to radiation accidents, terrorist attacks and national defense activities. The use of biodosimetry is a standard of care for the triage and treatment of radiation injuries. Resources and procedures need to be established to implement a multiple-parameter biodosimetry system coupled with expert medial guidance to provide an integrated radiation diagnostic system to meet USDOD requirements. Current USDOD biodosimetry capabilities were identified and recommendations to fill the identified gaps are provided...
December 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27837034/actinometric-and-biodosimetric-evaluation-of-uv-c-dose-delivery-in-annular-taylor-coutte-and-coiled-tube-continuous-systems
#8
Alexandra Müller, Marta Orlowska, Michael Knörr, Mario R Stahl, Ralf Greiner, Tatiana Koutchma
In this study, the evaluation of the performance of two thin-film UV-C reactors (annular and Taylor-Couette) and a coiled tube system is presented using actinometry and biodosimetry methods. The iodide/iodate actinometry method was found suitable for comparison of the efficiency of UV-C dose delivery of the UV-C continuous flow systems. Inactivation kinetics of Escherichia coli ATCC 8739 in quarter-strength Ringer's solution (absorption coefficient α254 nm ∼ 0 cm(-1)) at various flow conditions at Reynolds numbers in the range of 26 to 3000 showed a good correlation between the different reactor types...
November 11, 2016: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/27813725/dose-assessment-intercomparisons-within-the-reneb-network-using-g0-lymphocyte-prematurely-condensed-chromosomes-pcc-assay
#9
Georgia I Terzoudi, Gabriel Pantelias, Firouz Darroudi, Katarzyna Barszczewska, Iwona Buraczewska, Julie Depuydt, Dimka Georgieva, Valeria Hadjidekova, Vasiliki I Hatzi, Ioanna Karachristou, Maria Karakosta, Roberta Meschini, Radhia M'Kacher, Alegria Montoro, Fabrizio Palitti, Antonio Pantelias, Gaetano Pepe, Michelle Ricoul, Laure Sabatier, Natividad Sebastià, Sylwester Sommer, Anne Vral, Demetre Zafiropoulos, Andrzej Wojcik
PURPOSE: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G0-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. MATERIALS AND METHODS: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy γ-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals...
November 4, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27778526/the-reneb-operational-basis-complement-of-established-biodosimetric-assays
#10
Andrzej Wojcik, Ursula Oestreicher, Lleonard Barrios, Anne Vral, Georgia Terzoudi, Elizabeth Ainsbury, Kai Rothkamm, Francois Trompier, Ulrike Kulka
PURPOSE: To set up an operational basis of the Realizing the European Network of Biodosimetry (RENEB) network within which the application of seven established biodosimetric tools (the dicentric assay, the FISH assay, the micronucleus assay, the PCC assay, the gamma-H2AX assay, electron paramagnetic resonance and optically stimulated luminescence) will be compared and standardized among the participating laboratories. METHODOLOGY: Two intercomparisons were organized where blood samples and smartphone components were irradiated, coded and sent out to participating laboratories for dosimetric analysis...
October 25, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27766931/reneb-intercomparisons-applying-the-conventional-dicentric-chromosome-assay-dca
#11
Ursula Oestreicher, Daniel Samaga, Elizabeth Ainsbury, Ana Catarina Antunes, Ans Baeyens, Leonardo Barrios, Christina Beinke, Philip Beukes, William F Blakely, Alexandra Cucu, Andrea De Amicis, Julie Depuydt, Stefania De Sanctis, Marina Di Giorgio, Katalin Dobos, Inmaculada Dominguez, Pham Ngoc Duy, Marco E Espinoza, Farrah N Flegal, Markus Figel, Omar Garcia, Octávia Monteiro Gil, Eric Gregoire, C Guerrero-Carbajal, İnci Güçlü, Valeria Hadjidekova, Prakash Hande, Ulrike Kulka, Jennifer Lemon, Carita Lindholm, Florigio Lista, Katalin Lumniczky, Wilner Martinez-Lopez, Nataliya Maznyk, Roberta Meschini, Radia M'kacher, Alegria Montoro, Jayne Moquet, Mercedes Moreno, Mihaela Noditi, Jelena Pajic, Analía Radl, Michelle Ricoul, Horst Romm, Laurence Roy, Laure Sabatier, Natividad Sebastià, Jacobus Slabbert, Sylwester Sommer, Monica Stuck Oliveira, Uma Subramanian, Yumiko Suto, Tran Que, Antonella Testa, Georgia Terzoudi, Anne Vral, Ruth Wilkins, LusiYanti Yanti, Demetre Zafiropoulos, Andrzej Wojcik
PURPOSE: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network. MATERIALS AND METHODS: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants...
October 21, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27746851/liquid-handling-optimization-in-high-throughput-biodosimetry-tool
#12
Dakai Bian, Jason C Tsui, Mikhail Repin, Guy Garty, Helen Turner, Y Lawrence Yao, David J Brenner
Due to the need of high-speed and efficient biodosimetric assays for triage and therapy in the event of radiological or nuclear attack, a robotically based automated biodosimetry tool (RABiT) has been developed over the past few years. Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely, cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process...
December 2016: Journal of Medical Devices
https://www.readbyqxmd.com/read/27725377/standardization-of-calyculina-induced-pcc-assay-and-its-advantages-over-okadaic-acid-pcc-assay-in-biodosimetry-applications
#13
Rajesha K Nairy, Narayana Yerol, Nagesh N Bhat, Utkarsha Desai, Kapil Shirsath, Usha Yadav, Rajesh K Chaurasia, Sapra B K
OBJECTIVE: In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using (60)Co gamma radiation and 8 MeV pulsed electron beam for biodosimetry application. MATERIALS AND METHODS: The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes.Chemical treatment was given for the last 1 h of a 48 h culture...
November 29, 2016: Journal of Occupational Health
https://www.readbyqxmd.com/read/27710702/state-of-the-art-advances-in-radiation-biodosimetry-for-mass-casualty-events-involving-radiation-exposure
#14
Mary Sproull, Kevin Camphausen
With the possibility of large-scale terrorist attacks around the world, the need for modeling and development of new medical countermeasures for potential future chemical, biological, radiological and nuclear (CBRN) has been well established. Project Bioshield, initiated in 2004, provided a framework to develop and expedite research in the field of CBRN exposures. To respond to large-scale population exposures from a nuclear event or radiation dispersal device (RDD), new methods for determining received dose using biological modeling became necessary...
November 2016: Radiation Research
https://www.readbyqxmd.com/read/27705052/reneb-biodosimetry-intercomparison-analyzing-translocations-by-fish
#15
Joan Francesc Barquinero, Christina Beinke, Mireia Borràs, Iwona Buraczewska, Firouz Darroudi, Eric Gregoire, Rositsa Hristova, Ulrike Kulka, Carita Lindholm, Mercedes Moreno, Jayne Moquet, Ursula Oestreicher, M Jesús Prieto, Mònica Pujol, Michelle Ricoul, Laure Sabatier, Sylwester Sommer, Mingzhu Sun, Andrzej Wojcik, Leonardo Barrios
PURPOSE: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. MATERIALS AND METHODS: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions...
October 5, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27699012/lipopolysaccharide-binding-protein-is-efficient-in-biodosimetry-during-radiotherapy-of-lung-cancer
#16
Justyna Chalubinska-Fendler, Wojciech Fendler, Michal Spych, Krystyna Wyka, Jolanta Luniewska-Bury, Jacek Fijuth
The aim of the present study was to determine if the serum levels of early markers of inflammation, such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and lipopolysaccharide-binding protein (LBP) were correlated with the radiation dose received by the pulmonary and mediastinal structures of patients with non-small cell lung cancer (NSCLC). This pilot study included 26 patients with NSCLC who received total radiation doses ranging from 54 to 74 Gy (2.0 Gy/fraction). Cytokines were measured at baseline by enzyme-linked immunosorbant assay, and following administration of total doses of 20 and 40 Gy...
October 2016: Biomedical Reports
https://www.readbyqxmd.com/read/27686523/the-second-gamma-h2ax-assay-inter-comparison-exercise-carried-out-in-the-framework-of-the-european-biodosimetry-network-reneb
#17
Jayne Moquet, Stephen Barnard, Albena Staynova, Carita Lindholm, Octávia Monteiro Gil, Vanda Martins, Ute Rößler, Anne Vral, Charlot Vandevoorde, Maria Wojewódzka, Kai Rothkamm
PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with (60)Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner...
August 15, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27675952/nanofacs-extracellular-vesicle-subset-sorting-for-personalized-medicine-and-biodosimetry
#18
J C Jones, A Morales-Kastresana, A H Kesarwala, L Jenkins, P Choyke, K A Camphausen, J Berzofsky
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27673504/reneb-intercomparison-exercises-analyzing-micronuclei-cytokinesis-block-micronucleus-assay
#19
Julie Depuydt, Ans Baeyens, Stephen Barnard, Christina Beinke, Anett Benedek, Philip Beukes, Iwona Buraczewska, Firouz Darroudi, Stefania De Sanctis, Inmaculada Dominguez, Octávia Monteiro Gil, Valeria Hadjidekova, Enikő Kis, Ulrike Kulka, Florigio Lista, Katalin Lumniczky, Radhia M'kacher, Jayne Moquet, Doina Obreja, Ursula Oestreicher, Jelena Pajic, Nuria Pastor, Ljubomira Popova, Elisa Regalbuto, Michelle Ricoul, Laure Sabatier, Jacobus Slabbert, Sylwester Sommer, Antonella Testa, Hubert Thierens, Andrzej Wojcik, Anne Vral
PURPOSE: In the framework of the 'Realizing the European Network of Biodosimetry' (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. MATERIALS AND METHODS: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate...
August 15, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27590469/united-states-department-of-health-and-human-services-biodosimetry-and-radiological-nuclear-medical-countermeasure-programs
#20
Mary J Homer, Robert Raulli, Andrea L DiCarlo-Cohen, John Esker, Chad Hrdina, Bert W Maidment, Brian Moyer, Carmen Rios, Francesca Macchiarini, Pataje G Prasanna, Lynne Wathen
The United States Department of Health and Human Services (HHS) is fully committed to the development of medical countermeasures to address national security threats from chemical, biological, radiological, and nuclear agents. Through the Public Health Emergency Medical Countermeasures Enterprise, HHS has launched and managed a multi-agency, comprehensive effort to develop and operationalize medical countermeasures. Within HHS, development of medical countermeasures includes the National Institutes of Health (NIH), (led by the National Institute of Allergy and Infectious Diseases), the Office of the Assistant Secretary of Preparedness and Response/Biomedical Advanced Research and Development Authority (BARDA); with the Division of Medical Countermeasure Strategy and Requirements, the Centers for Disease Control and Prevention, and the Food and Drug Administration as primary partners in this endeavor...
September 2016: Radiation Protection Dosimetry
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