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https://www.readbyqxmd.com/read/27886989/u-s-department-of-defense-multiple-parameter-biodosimetry-network
#1
William F Blakely, Alexander Romanyukha, Selena M Hayes, Ricardo A Reyes, H Michael Stewart, Matthew H Hoefer, Anthony Williams, Thad Sharp, L Andrew Huff
The U.S. Department of Defense (USDOD) service members are at risk of exposure to ionizing radiation due to radiation accidents, terrorist attacks and national defense activities. The use of biodosimetry is a standard of care for the triage and treatment of radiation injuries. Resources and procedures need to be established to implement a multiple-parameter biodosimetry system coupled with expert medial guidance to provide an integrated radiation diagnostic system to meet USDOD requirements. Current USDOD biodosimetry capabilities were identified and recommendations to fill the identified gaps are provided...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27837034/actinometric-and-biodosimetric-evaluation-of-uv-c-dose-delivery-in-annular-taylor-coutte-and-coiled-tube-continuous-systems
#2
Alexandra Müller, Marta Orlowska, Michael Knörr, Mario R Stahl, Ralf Greiner, Tatiana Koutchma
In this study, the evaluation of the performance of two thin-film UV-C reactors (annular and Taylor-Couette) and a coiled tube system is presented using actinometry and biodosimetry methods. The iodide/iodate actinometry method was found suitable for comparison of the efficiency of UV-C dose delivery of the UV-C continuous flow systems. Inactivation kinetics of Escherichia coli ATCC 8739 in quarter-strength Ringer's solution (absorption coefficient α254 nm ∼ 0 cm(-1)) at various flow conditions at Reynolds numbers in the range of 26 to 3000 showed a good correlation between the different reactor types...
November 11, 2016: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/27813725/dose-assessment-intercomparisons-within-the-reneb-network-using-g0-lymphocyte-prematurely-condensed-chromosomes-pcc-assay
#3
Georgia I Terzoudi, Gabriel Pantelias, Firouz Darroudi, Katarzyna Barszczewska, Iwona Buraczewska, Julie Depuydt, Dimka Georgieva, Valeria Hadjidekova, Vasiliki I Hatzi, Ioanna Karachristou, Maria Karakosta, Roberta Meschini, Radhia M'Kacher, Alegria Montoro, Fabrizio Palitti, Antonio Pantelias, Gaetano Pepe, Michelle Ricoul, Laure Sabatier, Natividad Sebastià, Sylwester Sommer, Anne Vral, Demetre Zafiropoulos, Andrzej Wojcik
PURPOSE: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G0-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. MATERIALS AND METHODS: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy γ-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals...
November 4, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27778526/the-reneb-operational-basis-complement-of-established-biodosimetric-assays
#4
Andrzej Wojcik, Ursula Oestreicher, Lleonard Barrios, Anne Vral, Georgia Terzoudi, Elizabeth Ainsbury, Kai Rothkamm, Francois Trompier, Ulrike Kulka
PURPOSE: To set up an operational basis of the Realizing the European Network of Biodosimetry (RENEB) network within which the application of seven established biodosimetric tools (the dicentric assay, the FISH assay, the micronucleus assay, the PCC assay, the gamma-H2AX assay, electron paramagnetic resonance and optically stimulated luminescence) will be compared and standardized among the participating laboratories. METHODOLOGY: Two intercomparisons were organized where blood samples and smartphone components were irradiated, coded and sent out to participating laboratories for dosimetric analysis...
October 25, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27766931/reneb-intercomparisons-applying-the-conventional-dicentric-chromosome-assay-dca
#5
Ursula Oestreicher, Daniel Samaga, Elizabeth Ainsbury, Ana Catarina Antunes, Ans Baeyens, Leonardo Barrios, Christina Beinke, Philip Beukes, William F Blakely, Alexandra Cucu, Andrea De Amicis, Julie Depuydt, Stefania De Sanctis, Marina Di Giorgio, Katalin Dobos, Inmaculada Dominguez, Pham Ngoc Duy, Marco E Espinoza, Farrah N Flegal, Markus Figel, Omar Garcia, Octávia Monteiro Gil, Eric Gregoire, C Guerrero-Carbajal, İnci Güçlü, Valeria Hadjidekova, Prakash Hande, Ulrike Kulka, Jennifer Lemon, Carita Lindholm, Florigio Lista, Katalin Lumniczky, Wilner Martinez-Lopez, Nataliya Maznyk, Roberta Meschini, Radia M'kacher, Alegria Montoro, Jayne Moquet, Mercedes Moreno, Mihaela Noditi, Jelena Pajic, Analía Radl, Michelle Ricoul, Horst Romm, Laurence Roy, Laure Sabatier, Natividad Sebastià, Jacobus Slabbert, Sylwester Sommer, Monica Stuck Oliveira, Uma Subramanian, Yumiko Suto, Tran Que, Antonella Testa, Georgia Terzoudi, Anne Vral, Ruth Wilkins, LusiYanti Yanti, Demetre Zafiropoulos, Andrzej Wojcik
PURPOSE: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network. MATERIALS AND METHODS: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants...
October 21, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27746851/liquid-handling-optimization-in-high-throughput-biodosimetry-tool
#6
Dakai Bian, Jason C Tsui, Mikhail Repin, Guy Garty, Helen Turner, Y Lawrence Yao, David J Brenner
Due to the need of high-speed and efficient biodosimetric assays for triage and therapy in the event of radiological or nuclear attack, a robotically based automated biodosimetry tool (RABiT) has been developed over the past few years. Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely, cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process...
December 2016: Journal of Medical Devices
https://www.readbyqxmd.com/read/27725377/standardization-of-calyculina-induced-pcc-assay-and-its-advantages-over-okadaic-acid-pcc-assay-in-biodosimetry-applications
#7
Rajesha K Nairy, Narayana Yerol, Nagesh N Bhat, Utkarsha Desai, Kapil Shirsath, Usha Yadav, Rajesh K Chaurasia, Sapra B K
OBJECTIVE: In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using (60)Co gamma radiation and 8 MeV pulsed electron beam for biodosimetry application. MATERIALS AND METHODS: The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes.Chemical treatment was given for the last 1 h of a 48 h culture...
November 29, 2016: Journal of Occupational Health
https://www.readbyqxmd.com/read/27710702/state-of-the-art-advances-in-radiation-biodosimetry-for-mass-casualty-events-involving-radiation-exposure
#8
Mary Sproull, Kevin Camphausen
With the possibility of large-scale terrorist attacks around the world, the need for modeling and development of new medical countermeasures for potential future chemical, biological, radiological and nuclear (CBRN) has been well established. Project Bioshield, initiated in 2004, provided a framework to develop and expedite research in the field of CBRN exposures. To respond to large-scale population exposures from a nuclear event or radiation dispersal device (RDD), new methods for determining received dose using biological modeling became necessary...
November 2016: Radiation Research
https://www.readbyqxmd.com/read/27705052/reneb-biodosimetry-intercomparison-analyzing-translocations-by-fish
#9
Joan Francesc Barquinero, Christina Beinke, Mireia Borràs, Iwona Buraczewska, Firouz Darroudi, Eric Gregoire, Rositsa Hristova, Ulrike Kulka, Carita Lindholm, Mercedes Moreno, Jayne Moquet, Ursula Oestreicher, M Jesús Prieto, Mònica Pujol, Michelle Ricoul, Laure Sabatier, Sylwester Sommer, Mingzhu Sun, Andrzej Wojcik, Leonardo Barrios
PURPOSE: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. MATERIALS AND METHODS: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions...
October 5, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27699012/lipopolysaccharide-binding-protein-is-efficient-in-biodosimetry-during-radiotherapy-of-lung-cancer
#10
Justyna Chalubinska-Fendler, Wojciech Fendler, Michal Spych, Krystyna Wyka, Jolanta Luniewska-Bury, Jacek Fijuth
The aim of the present study was to determine if the serum levels of early markers of inflammation, such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and lipopolysaccharide-binding protein (LBP) were correlated with the radiation dose received by the pulmonary and mediastinal structures of patients with non-small cell lung cancer (NSCLC). This pilot study included 26 patients with NSCLC who received total radiation doses ranging from 54 to 74 Gy (2.0 Gy/fraction). Cytokines were measured at baseline by enzyme-linked immunosorbant assay, and following administration of total doses of 20 and 40 Gy...
October 2016: Biomedical Reports
https://www.readbyqxmd.com/read/27686523/the-second-gamma-h2ax-assay-inter-comparison-exercise-carried-out-in-the-framework-of-the-european-biodosimetry-network-reneb
#11
Jayne Moquet, Stephen Barnard, Albena Staynova, Carita Lindholm, Octávia Monteiro Gil, Vanda Martins, Ute Rößler, Anne Vral, Charlot Vandevoorde, Maria Wojewódzka, Kai Rothkamm
PURPOSE: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma-H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. MATERIALS AND METHODS: Whole blood and separated lymphocyte samples were homogenously irradiated with (60)Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner...
August 15, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27675952/nanofacs-extracellular-vesicle-subset-sorting-for-personalized-medicine-and-biodosimetry
#12
J C Jones, A Morales-Kastresana, A H Kesarwala, L Jenkins, P Choyke, K A Camphausen, J Berzofsky
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27673504/reneb-intercomparison-exercises-analyzing-micronuclei-cytokinesis-block-micronucleus-assay
#13
Julie Depuydt, Ans Baeyens, Stephen Barnard, Christina Beinke, Anett Benedek, Philip Beukes, Iwona Buraczewska, Firouz Darroudi, Stefania De Sanctis, Inmaculada Dominguez, Octávia Monteiro Gil, Valeria Hadjidekova, Enikő Kis, Ulrike Kulka, Florigio Lista, Katalin Lumniczky, Radhia M'kacher, Jayne Moquet, Doina Obreja, Ursula Oestreicher, Jelena Pajic, Nuria Pastor, Ljubomira Popova, Elisa Regalbuto, Michelle Ricoul, Laure Sabatier, Jacobus Slabbert, Sylwester Sommer, Antonella Testa, Hubert Thierens, Andrzej Wojcik, Anne Vral
PURPOSE: In the framework of the 'Realizing the European Network of Biodosimetry' (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. MATERIALS AND METHODS: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate...
August 15, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27590469/united-states-department-of-health-and-human-services-biodosimetry-and-radiological-nuclear-medical-countermeasure-programs
#14
Mary J Homer, Robert Raulli, Andrea L DiCarlo-Cohen, John Esker, Chad Hrdina, Bert W Maidment, Brian Moyer, Carmen Rios, Francesca Macchiarini, Pataje G Prasanna, Lynne Wathen
The United States Department of Health and Human Services (HHS) is fully committed to the development of medical countermeasures to address national security threats from chemical, biological, radiological, and nuclear agents. Through the Public Health Emergency Medical Countermeasures Enterprise, HHS has launched and managed a multi-agency, comprehensive effort to develop and operationalize medical countermeasures. Within HHS, development of medical countermeasures includes the National Institutes of Health (NIH), (led by the National Institute of Allergy and Infectious Diseases), the Office of the Assistant Secretary of Preparedness and Response/Biomedical Advanced Research and Development Authority (BARDA); with the Division of Medical Countermeasure Strategy and Requirements, the Centers for Disease Control and Prevention, and the Food and Drug Administration as primary partners in this endeavor...
September 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27584947/overview-of-physical-dosimetry-methods-for-triage-application-integrated-in-the-new-european-network-reneb
#15
François Trompier, Christopher Burbidge, Céline Bassinet, Marion Baumann, Emanuela Bortolin, Cinzia De Angelis, Jonathan Eakins, Sara Della Monaca, Paola Fattibene, Maria Cristina Quattrini, Rick Tanner, Albrecht Wieser, Clemens Woda
PURPOSE: In the EC-funded project RENEB (Realizing the European Network in Biodosimetry), physical methods applied to fortuitous dosimetric materials are used to complement biological dosimetry, to increase dose assessment capacity for large-scale radiation/nuclear accidents. This paper describes the work performed to implement Optically Stimulated Luminescence (OSL) and Electron Paramagnetic Resonance (EPR) dosimetry techniques. MATERIALS AND METHODS: OSL is applied to electronic components and EPR to touch-screen glass from mobile phones...
September 12, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27564973/culture-time-and-reagent-minimization-in-the-chemical-pcc-assay
#16
Ivonne Romero, Ana Ilsa Lamadrid, Jorge Ernesto González, Tania Mandina, Omar García
The possibility to reduce the culture time and volume of blood and reagents required for the chemical Premature Chromosome Condensation (PCC) assay is demonstrated in this work. Peripheral whole blood was exposed to gamma radiation (1-20 Gy). Lymphocytes were cultured for 40 h, using 50 μl of blood and 450 μl of culture medium. The dose-response curves were adjusted, using length ratio (LR) of the longest to the shortest chromosome piece, and the frequency of rings per cell. No statistical differences were found between the results obtained with this method and those reported with the classical PCC assay culture...
October 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27557790/capabilities-of-the-reneb-network-for-research-and-large-scale-radiological-and-nuclear-emergency-situations
#17
Octávia Monteiro Gil, Pedro Vaz, Horst Romm, Cinzia De Angelis, Ana Catarina Antunes, Joan-Francesc Barquinero, Christina Beinke, Emanuela Bortolin, Christopher Ian Burbidge, Alexandra Cucu, Sara Della Monaca, Mercedes Moreno Domene, Paola Fattibene, Eric Gregoire, Valeria Hadjidekova, Ulrike Kulka, Carita Lindholm, Roberta Meschini, Radhia M'Kacher, Jayne Moquet, Ursula Oestreicher, Fabrizio Palitti, Gabriel Pantelias, Alegria Montoro Pastor, Irina-Anca Popescu, Maria Cristina Quattrini, Michelle Ricoul, Kai Rothkamm, Laure Sabatier, Natividad Sebastià, Sylwester Sommer, Georgia Terzoudi, Antonella Testa, François Trompier, Anne Vral
PURPOSE: To identify and assess, among the participants in the RENEB (Realizing the European Network of Biodosimetry) project, the emergency preparedness, response capabilities and resources that can be deployed in the event of a radiological or nuclear accident/incident affecting a large number of individuals. These capabilities include available biodosimetry techniques, infrastructure, human resources (existing trained staff), financial and organizational resources (including the role of national contact points and their articulation with other stakeholders in emergency response) as well as robust quality control/assurance systems...
October 4, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27556353/transcript-analysis-for-internal-biodosimetry-using-peripheral-blood-from-neuroblastoma-patients-treated-with-131-i-mibg-a-targeted-radionuclide
#18
David A Edmondson, Erin E Karski, Ayano Kohlgruber, Harsha Koneru, Katherine K Matthay, Shelly Allen, Christine L Hartmann, Leif E Peterson, Steven G DuBois, Matthew A Coleman
Calculating internal dose from therapeutic radionuclides currently relies on estimates made from multiple radiation exposure measurements, converted to absorbed dose in specific organs using the Medical Internal Radiation Dose (MIRD) schema. As an alternative biodosimetric approach, we utilized gene expression analysis of whole blood from patients receiving targeted radiotherapy. Collected blood from patients with relapsed or refractory neuroblastoma who received (131)I-labeled metaiodobenzylguanidine ((131)I-mIBG) at the University of California San Francisco (UCSF) was used to compare calculated internal dose with the modulation of chosen gene expression...
September 2016: Radiation Research
https://www.readbyqxmd.com/read/27547893/web-based-scoring-is-useful-for-validation-and-harmonisation-of-scoring-criteria-within-reneb
#19
Horst Romm, Elizabeth A Ainsbury, Joan Francesc Barquinero, Leonardo Barrios, Christina Beinke, Alexandra Cucu, Mercedes Moreno Domene, Silvia Filippi, Octávia Monteiro Gil, Eric Gregoire, Valeria Hadjidekova, Vasia Hatzi, Carita Lindholm, Radhia M Kacher, Alegria Montoro, Jayne Moquet, Mihaela Noditi, Ursula Oestreicher, Fabrizio Palitti, Gabriel Pantelias, María Jesús Prieto, Irina Popescu, Kai Rothkamm, Natividad Sebastià, Sylwester Sommer, Georgia Terzoudi, Antonella Testa, Andrzej Wojcik
PURPOSE: To establish a training data set of digital images and to investigate the scoring criteria and dose assessment of the dicentric assay within the European network of biodosimetry (RENEB), a web based scoring inter-comparison was undertaken by 17 RENEB partners. MATERIALS AND METHODS: Two sets of 50 high resolution images were uploaded onto the RENEB website. One set included metaphases after a moderate exposure (1.3 Gy) and the other set consisted of metaphases after a high dose exposure (3...
August 22, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27524557/foundations-of-identifying-individual-chromosomes-by-imaging-flow-cytometry-with-applications-in-radiation-biodosimetry
#20
Lindsay A Beaton-Green, Matthew A Rodrigues, Sylvie Lachapelle, Ruth C Wilkins
Biodosimetry is an important tool for triage in the case of large-scale radiological or nuclear emergencies, but traditional microscope-based methods can be tedious and prone to scorer fatigue. While the dicentric chromosome assay (DCA) has been adapted for use in triage situations, it is still time-consuming to create and score slides. Recent adaptations of traditional biodosimetry assays to imaging flow cytometry (IFC) methods have dramatically increased throughput. Additionally, recent improvements in image analysis algorithms in IFC software have resulted in improved specificity for spot counting of small events...
August 11, 2016: Methods: a Companion to Methods in Enzymology
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