Cheng Shen, Meng Meng, Jiafeng Jin, Xiaodan Hu, Manman Lin, Meijing Gao, Xiao Zhang, Qing Zhu, Chongxin Xu, Yajing Xie, Chengyu Chen, Zhiyong Zhang, Guojing Che, Ofentse Jacob Pooe, Yuan Liu, Xianjin Liu
Bacillus thuringiensis (Bt) Cry toxins have been widely used in the development of genetically modified organisms (GMOs) for pest control. This work aimed to establish more cost effective and broader detection methods for commonly used Cry toxins. Using ligand blot and bio-layer interferometry, we confirmed that a recombinant toxin-binding fragments derived from Helicoverpa armigera cadherin-like protein (HaCad-TBR) could broadly bind Cry1Ab, Cry1Ac, Cry2Aa, and Cry2Ab with the affinity of 0.149, 0.402, 120, and 4...
November 14, 2023: International Journal of Biological Macromolecules