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Gene pvl and Meca

Happiness H Kumburu, Tolbert Sonda, Pimlapas Leekitcharoenphon, Marco van Zwetselaar, Oksana Lukjancenko, Michael Alifrangis, Ole Lund, Blandina T Mmbaga, Gibson Kibiki, Frank M Aarestrup
Objective: To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. Methods: DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end 2 × 250 bp protocol. Raw sequence data were analyzed using online tools. Results: Full susceptibility to vancomycin and chloramphenicol was observed...
2018: BioMed Research International
Maryam Ghahremani, Nima Hosseini Jazani, Yaeghob Sharifi
OBJECTIVES: This study aimed to evaluate the frequency, the, phenotypic and molecular characteristics of methicillin- resistant, Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA), from clinical specimens at three university teaching hospitals in Urmia-North West of Iran (2012- 2015). METHODS: After identification of the isolates, the antibiotic, susceptibility, the presence of mecA, vanA, pvl genes and staphylococcal, cassette chromosome mec (SCCmec) typing were evaluated...
February 14, 2018: Journal of Global Antimicrobial Resistance
M Angeles Argudín, A Deplano, S Vandendriessche, M Dodémont, C Nonhoff, O Denis, S Roisin
Studies based on genome-wide single nucleotide polymorphisms (SNPs) supported the existence of two subpopulations in clonal complex (CC) 398 Staphylococcus aureus: an ancestral human-adapted clade (HC) and an animal-associated clade (AC). In this study, we have investigated the occurrence of genetic markers that allow discrimination of these subpopulations among CC398 isolates collected during 2014 to 2016 from human patients in Belgium. A collection of isolates was investigated by means of spa-typing and 16S-mecA-nuc PCR...
February 15, 2018: European Journal of Clinical Microbiology & Infectious Diseases
S Johler, G Macori, A Bellio, P L Acutis, S Gallina, L Decastelli
Staphylococcus aureus is a common cause of food-borne intoxications. Several staphylococcal food poisoning outbreaks have been linked to consumption of raw milk cheeses and artisanal cheese production. However, information on Staph. aureus isolated from artisanal raw milk cheeses and small-scale dairy production environments is very limited. Therefore, we aimed to characterize Staph. aureus isolated along the artisanal raw milk production chain by determining (1) the population structure, and (2) the presence/absence of enterotoxin genes, mecA/C, and pvl...
January 31, 2018: Journal of Dairy Science
Carla Tomatis, María R Baroni, María A Mendosa, Alicia Nagel, Analia Mollerach, Claudia Alvarez, Maria Laura Zurbriggen, Sabrina Cristobal, Glenda Segovia, Emilce de Los A Méndez
Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized patients, whereas MRSA in SST. SCCmec type IV was predominant, followed by type I...
January 11, 2018: Revista Argentina de Microbiología
Parisa Sabbagh, Amirmorteza Ebrahimzadeh-Namvar, Elaheh Ferdosi-Shahandashti, Mostafa Javanian, Soraya Khafri, Mehdi Rajabnia
Background: Staphylococcus aureus (s. aureus) nasal carriers, particularly the healthcare staff can be considered as a potential source for the spread of resistant strains. The aim of this study was to determine the molecular characterization of S. aureus strains isolated among the staff nasal carriers in one of the teaching hospitals in Babol. Methods: A total of 120 nasal swabs were taken from the staff of Ayatollah Rouhani Hospital Babol during 2016. The antibiotic resistance pattern was performed by disc diffusion method for 13 antibiotics, including cefoxitin, cephalothin, teicoplanin, vancomycin, daptomycin, oxacillin, amoxicillin, amikacin, linezolid, ciprofloxacin, levofloxacin, erythromycin, rifampin, according to the CLSI 2015...
2017: Caspian Journal of Internal Medicine
Dewi Santosaningsih, Sanarto Santoso, Nanik Setijowati, Harun A Rasyid, Nyoman S Budayanti, Ketut Suata, Dicky B Widhyatmoko, Priyo B Purwono, Kuntaman Kuntaman, Damayanti Damayanti, Cita R S Prakoeswa, Mitchell Laurens, Josephine W I van Nierop, Geraldine L Nanninga, Neline Oudenes, Michelle de Regt, Susan V Snijders, Henri A Verbrugh, Juliëtte A Severin
OBJECTIVES: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. METHODS: Staphylococcus aureus were cultured from anterior nares, throat and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S...
January 2018: Tropical Medicine & International Health: TM & IH
Alice Gonçalves Martins Gonzalez, Leila Márcia Peres Marques, Marcel da Silva Amorim Gomes, JhonathanCampos do Couto Beltrão, Marcos Gabriel Pinheiro, Luciana Maria Ramires Esper, Geraldo Renato de Paula, Lenise Arneiro Teixeira, Fábio Aguiar-Alves
This study aimed to investigate classical enterotoxin (sea to see) and mecA genes, by polymerase chain reaction (PCR) and anitimicrobial susceptibility, by disc diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin resistant S. aureus (MRSA) isolates were investigated for the presence of PVL genes and clonal diversity. Thirty-one S. aureus were isolated from four MFC samples. Seven (22.6%) S. aureus carried mecA gene and two of them carried enterotoxin genes seb/sec and sea/seb...
November 1, 2017: FEMS Microbiology Letters
Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis Kollannur, Radhika Syam
Dairy cows affected with subclinical mastitis can be sources of virulent, antimicrobial-resistant Staphylococci to humans because of the excretion of the bacteria through their milk. This study focussed on the phenotypic and genotypic antibiotic resistance patterns of Staphylococci isolated from dairy cows in early dry period. Among 96 isolates of Gram positive cocci from 157 cows, 76 were identified as Coagulase Negative Staphylococci and the remaining 20 were Staphylococcus aureus. Typical amplicons of coagulase gene were obtained for all 20 samples of S...
2017: PloS One
Muhammad Ali Syed, Syeda Hala Hussain Shah, Yasmin Sherafzal, Syed Shafi-Ur-Rehman, Mushtaq Ahmed Khan, John B Barrett, Tiffanie A Woodley, Bushra Jamil, Shahid Ahmad Abbasi, Charlene R Jackson
Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria monocytogenes, and Yersinia enterocolitica have already been reported from table eggs. More important is the presence of antimicrobial-resistant bacterial strains in this food source...
October 25, 2017: Foodborne Pathogens and Disease
Amal Awad, Hazem Ramadan, Sherif Nasr, Ahmed Ateya, Samar Atwa
BACKGROUND AND OBJECTIVE: Staphylococcus aureus is commonly associated with mastitis in dairy herds with potential public health implications. This study was conducted to investigate the existence of S. aureus in mastitic milk and to determine the antimicrobial resistance profiles of the isolated strains as well as the resistance and virulence associated genes. MATERIALS AND METHODS: Two hundred quarter milk samples were collected from 3 dairy farms at Dakahliya (n = 2) and Damietta (n = 1) Governorates, Egypt from September to December 2016...
2017: Pakistan Journal of Biological Sciences: PJBS
Panagiotis Papadopoulos, Theofilos Papadopoulos, Apostolos S Angelidis, Evridiki Boukouvala, Antonios Zdragas, Anna Papa, Christos Hadjichristodoulou, Daniel Sergelidis
The objective of this study was to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the production chain of dairy products. Of 367 tested samples (36 bulk tank milk (BTM), 19 dairy products, 72 human, 185 animal, 55 equipment), 212 (57.8%) were found positive for S. aureus. Almost all isolates (99.6%) were resistant to at least one antimicrobial and 13.3% were multi-drug resistant (MDR), exhibiting resistance to three or more antibiotic classes. Eleven samples (3%) were found contaminated by MRSA carrying the mecA gene...
February 2018: Food Microbiology
Mehrdad Halaji, Ashkan Karimi, Parisa Shoaei, Mohammadreza Nahaei, Farzin Khorvash, Behrooz Ataei, Majid Yaran, Seyed Asghar Havaei
INTRODUCTION: Coagulase Negative Staphylococcus (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients. AIM: The aim of this study was to identify the types of Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin (PVL) gene among clinical Methicillin-Resistant S. epidermidis (MRSE) isolates collected from Isfahan. MATERIALS AND METHODS: This cross-sectional study was performed from March 2014 to January 2015 at a tertiary care hospital of Isfahan, Iran...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
Sara Giordana Rimoldi, Cristina Pagani, Erika Longhi, Valentina Di Cristo, Annamaria Di Gregorio, Alessandro Mancon, Pietro Zerbi, Cristina Gervasoni, Maria Rita Gismondo, Agostino Riva
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations were performed to confirm the presence of the mecA and PVL genes and to assign the SCCmec type, sequence type (ST) and clonal relatedness. A strain of PVL-producing methicillin-resistant S. aureus (MRSA) was responsible for infection in two related families (A and B), and a third family (C) was infected with PVL-producing methicillin-sensitive S...
September 5, 2017: Journal of Infection and Public Health
Matthaios Papadimitriou-Olivgeris, Eleanna Drougka, Fotini Fligou, Vasiliki Dodou, Fevronia Kolonitsiou, Kriton S Filos, Evangelos D Anastassiou, Efthimia Petinaki, Markos Marangos, Iris Spiliopoulou
Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton-Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton-Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR...
September 4, 2017: Toxins
Huimin Liu, Songli Li, Lu Meng, Lei Dong, Shengguo Zhao, Xinyi Lan, Jiaqi Wang, Nan Zheng
Staphylococcus aureus is one of the main pathogens involved in dairy cow mastitis. Monitoring of antibiotic use would prove useful to assess the risk of Staph. aureus in raw milk. The objective of this work was to investigate the prevalence of Staph. aureus strais isolated from raw milk in northern China, and to characterize antimicrobial susceptibility of these strains and their key virulence genes. In total, 195 raw milk samples were collected from 195 dairy farms located in 4 cities of northern China from May to September 2015...
November 2017: Journal of Dairy Science
Maryam Rahimpour Hesari, Ali Salehzadeh, Reza Kazemi Darsanaki
Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals...
September 1, 2017: Acta Microbiologica et Immunologica Hungarica
Seyed Asghar Havaei, Farkhondeh Poursina, Maryam Ahmadpour, Seyed Roholla Havaei, Meisam Ruzbahani
BACKGROUND: Panton-Valentine leukocidin (PVL) is a gamma-toxin produced by Staphylococcus aureus encoded by genes lukS/lukF-PV with several single-nucleotide polymorphisms. A mutation at nucleotide position 527 results in substitution of histidine (H) to arginine (R) at amino acid 176. The groups defined based on the amino acid change, the "R isoform" group and the "H isoform" group. The purpose of this study was to determine the frequency of PVL gene isoforms in S. aureus strains isolated from patients at Al-Zahra Hospital Isfahan and molecular characterization of PVL-positive methicillin-resistant S...
2017: Advanced Biomedical Research
Björn Bengtsson, Lotta Persson, Kerstin Ekström, Helle Ericsson Unnerstad, Henrik Uhlhorn, Stefan Börjesson
We investigated the occurrence of mecC-MRSA in wild hedgehogs (Erinaceus europaeus) in Sweden and characterized the obtained isolates. Samples from 55 hedgehogs from five counties of Sweden were cultivated selectively for MRSA and putative isolates were confirmed by real-time PCR detecting mecA, mecC, nuc and PVL genes. mecC-MRSA was confirmed in 35 (64%) animals from three geographically separated counties. Confirmed isolates were spa-typed and tested for antimicrobial susceptibility by broth microdilution...
August 2017: Veterinary Microbiology
Yunjia Song, Lanqing Cui, Yuan Lv, Yun Li, Feng Xue
OBJECTIVES: The aim of this study was to investigate the prevalence and molecular characteristics of clinical oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) isolates in China from July 2009 to June 2014. METHODS: A total of 2068 non-duplicate S. aureus isolates were collected from 21 hospitals. Antimicrobial susceptibility testing was performed by the agar dilution method. All OS-MRSA strains were screened for the presence of the genes mecA, mecC and nuc as well as the Panton-Valentine leukocidin gene (pvl)...
December 2017: Journal of Global Antimicrobial Resistance
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