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https://www.readbyqxmd.com/read/29746375/prevalence-and-molecular-characterization-of-s-aureus-and-mrsa-on-children-s-playgrounds
#1
Dipendra Thapaliya, Jhalka Kadariya, Mike Capuano, Haleigh Rush, Clair Yee, Mark Oet, Sapana Lohani, Tara C Smith
BACKGROUND: Staphylococcus aureus is a major public health concern due to the emergence of virulent and drug resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). Although numerous studies have been conducted to assess the environmental contamination of S. aureus in health-care and household settings, little is known about the prevalence and epidemiology of S. aureus, including MRSA, on environmental surfaces of children's playgrounds. This study investigated the prevalence and molecular epidemiology of S...
May 9, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29685228/phenotypic-and-genotypic-characteristics-of-staphylococcus-aureus-isolates-from-zoo-and-wild-animals
#2
Andrea T Feßler, Patricia Thomas, Kristin Mühldorfer, Mirjam Grobbel, Julian Brombach, Inga Eichhorn, Stefan Monecke, Ralf Ehricht, Stefan Schwarz
Antimicrobial resistance of Staphylococcus aureus is a major problem in human and veterinary medicine. The aim of this study was to characterise S. aureus isolates from wild and zoo animals mainly associated with bacterial infections. In total, 23 S. aureus isolates, including nine from wild animals and 14 from zoo animals, were obtained during routine diagnostics. All isolates were subjected to multilocus sequence typing (MLST), spa typing, macrorestriction analysis with subsequent SmaI pulsed-field gelelectrophoresis (PFGE), antimicrobial susceptibility testing and S...
May 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29675779/antibiotic-resistance-and-molecular-characteristics-of-staphylococcus-aureus-isolated-from-backyard-raised-pigs-and-pig-workers
#3
Asabe Halimat Momoh, Jacob K P Kwaga, Mohammed Bello, Anthony K B Sackey, Anders Rhod Larsen
Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes...
April 19, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29568515/prevalence-and-molecular-characterization-of-staphylococcus-aureus-from-human-stool-samples
#4
A E Kates, D Thapaliya, T C Smith, M L Chorazy
Background: To determine the prevalence of intestinal S. aureus colonization of patients at a large teaching hospital and determine the molecular characteristics of the identified strains. The second objective of this research was to determine risk factors associated with S. aureus intestinal colonization. Methods: A cross-sectional study of 781 specimens from inpatients and outpatients at the University of Iowa Hospitals and Clinics Clinical Microbiology Laboratory was conducted...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29564946/virulence-factor-genes-in-staphylococcus-aureus-isolated-from-diabetic-foot-soft-tissue-and-bone-infections
#5
Gerardo Víquez-Molina, Javier Aragón-Sánchez, Cristian Pérez-Corrales, Christian Murillo-Vargas, María Eugenia López-Valverde, Benjamin A Lipsky
The aim of this study is to describe the presence of genes encoding for 4 virulence factors (pvl, eta, etb, and tsst), as well as the mecA gene conferring resistance to beta-lactam antibiotics, in patients with diabetes and a staphylococcal foot infection. We have also analyzed whether isolates of Staphylococcus aureus from bone infections have a different profile for these genes compared with those from exclusively soft tissue infections. In this cross-sectional study of a prospectively recruited series of patients admitted to the Diabetic Foot Unit, San Juan de Dios Hospital, San José, Costa Rica with a moderate or severe diabetic foot infection (DFI), we collected samples from infected soft tissue and from bone during debridement...
March 1, 2018: International Journal of Lower Extremity Wounds
https://www.readbyqxmd.com/read/29487865/hospital-epidemiology-of-methicillin-resistant-staphylococcus-aureus-in-a-tertiary-care-hospital-in-moshi-tanzania-as-determined-by-whole-genome-sequencing
#6
Happiness H Kumburu, Tolbert Sonda, Pimlapas Leekitcharoenphon, Marco van Zwetselaar, Oksana Lukjancenko, Michael Alifrangis, Ole Lund, Blandina T Mmbaga, Gibson Kibiki, Frank M Aarestrup
Objective: To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. Methods: DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end 2 × 250 bp protocol. Raw sequence data were analyzed using online tools. Results: Full susceptibility to vancomycin and chloramphenicol was observed...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29454049/emergence-of-vancomycin-intermediate-and-resistant-staphylococcus-aureus-among-methicillin-resistant-s-aureus-isolated-from-clinical-specimens-in-the-northwest-of-iran
#7
Maryam Ghahremani, Nima Hosseini Jazani, Yaeghob Sharifi
OBJECTIVES: This study aimed to evaluate the frequency, the, phenotypic and molecular characteristics of methicillin- resistant, Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA), from clinical specimens at three university teaching hospitals in Urmia-North West of Iran (2012- 2015). METHODS: After identification of the isolates, the antibiotic, susceptibility, the presence of mecA, vanA, pvl genes and staphylococcal, cassette chromosome mec (SCCmec) typing were evaluated...
February 14, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29450768/cc398-staphylococcus-aureus-subpopulations-in-belgian-patients
#8
M Angeles Argudín, A Deplano, S Vandendriessche, M Dodémont, C Nonhoff, O Denis, S Roisin
Studies based on genome-wide single nucleotide polymorphisms (SNPs) supported the existence of two subpopulations in clonal complex (CC) 398 Staphylococcus aureus: an ancestral human-adapted clade (HC) and an animal-associated clade (AC). In this study, we have investigated the occurrence of genetic markers that allow discrimination of these subpopulations among CC398 isolates collected during 2014 to 2016 from human patients in Belgium. A collection of isolates was investigated by means of spa-typing and 16S-mecA-nuc PCR...
May 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29397175/short-communication-characterization-of-staphylococcus-aureus-isolated-along-the-raw-milk-cheese-production-process-in-artisan-dairies-in-italy
#9
S Johler, G Macori, A Bellio, P L Acutis, S Gallina, L Decastelli
Staphylococcus aureus is a common cause of food-borne intoxications. Several staphylococcal food poisoning outbreaks have been linked to consumption of raw milk cheeses and artisanal cheese production. However, information on Staph. aureus isolated from artisanal raw milk cheeses and small-scale dairy production environments is very limited. Therefore, we aimed to characterize Staph. aureus isolated along the artisanal raw milk production chain by determining (1) the population structure, and (2) the presence/absence of enterotoxin genes, mecA/C, and pvl...
April 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29336909/-non-reported-spa-types-in-our-country-in-staphylococcus-aureus-from-adult-patients-of-a-school-hospital-santa-fe-argentina
#10
Carla Tomatis, María R Baroni, María A Mendosa, Alicia Nagel, Analia Mollerach, Claudia Alvarez, Maria Laura Zurbriggen, Sabrina Cristobal, Glenda Segovia, Emilce de Los A Méndez
Staphylococcus aureus is a pathogen associated a different kind of infection. Molecular markers are useful tools to study microbial epidemiology. Twenty two methicillin-resistant S. aureus (MRSA) and 23 methicillin-susceptible S. aureus (MSSA) were studied by mecA gene, SCCmec cassette, Panton Valentine leucocidin (PVL) and spa polymorphism. The clinical data patients were analyzed. MSSA was prevalent in samples different from skin and soft tissue (SST) and in hospitalized patients, whereas MRSA in SST. SCCmec type IV was predominant, followed by type I...
January 11, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29201324/molecular-characterization-of-staphylococcus-aureus-strains-isolated-among-hospital-staff-nasal-carriers-of-babol-iran
#11
Parisa Sabbagh, Amirmorteza Ebrahimzadeh-Namvar, Elaheh Ferdosi-Shahandashti, Mostafa Javanian, Soraya Khafri, Mehdi Rajabnia
Background: Staphylococcus aureus (s. aureus) nasal carriers, particularly the healthcare staff can be considered as a potential source for the spread of resistant strains. The aim of this study was to determine the molecular characterization of S. aureus strains isolated among the staff nasal carriers in one of the teaching hospitals in Babol. Methods: A total of 120 nasal swabs were taken from the staff of Ayatollah Rouhani Hospital Babol during 2016. The antibiotic resistance pattern was performed by disc diffusion method for 13 antibiotics, including cefoxitin, cephalothin, teicoplanin, vancomycin, daptomycin, oxacillin, amoxicillin, amikacin, linezolid, ciprofloxacin, levofloxacin, erythromycin, rifampin, according to the CLSI 2015...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/29124834/prevalence-and-characterisation-of-staphylococcus-aureus-causing-community-acquired-skin-and-soft-tissue-infections-on-java-and-bali-indonesia
#12
Dewi Santosaningsih, Sanarto Santoso, Nanik Setijowati, Harun A Rasyid, Nyoman S Budayanti, Ketut Suata, Dicky B Widhyatmoko, Priyo B Purwono, Kuntaman Kuntaman, Damayanti Damayanti, Cita R S Prakoeswa, Mitchell Laurens, Josephine W I van Nierop, Geraldine L Nanninga, Neline Oudenes, Michelle de Regt, Susan V Snijders, Henri A Verbrugh, Juliëtte A Severin
OBJECTIVES: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. METHODS: Staphylococcus aureus were cultured from anterior nares, throat and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S...
January 2018: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29099921/methicillin-resistant-staphylococcus-aureus-in-minas-frescal-cheese-evaluation-of-classic-enterotoxin-genes-antimicrobial-resistance-and-clonal-diversity
#13
Alice Gonçalves Martins Gonzalez, Leila Márcia Peres Marques, Marcel da Silva Amorim Gomes, JhonathanCampos do Couto Beltrão, Marcos Gabriel Pinheiro, Luciana Maria Ramires Esper, Geraldo Renato de Paula, Lenise Arneiro Teixeira, Fábio Aguiar-Alves
This study aimed to investigate classical enterotoxin (sea to see) and mecA genes, by polymerase chain reaction (PCR) and anitimicrobial susceptibility, by disc diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin resistant S. aureus (MRSA) isolates were investigated for the presence of PVL genes and clonal diversity. Thirty-one S. aureus were isolated from four MFC samples. Seven (22.6%) S. aureus carried mecA gene and two of them carried enterotoxin genes seb/sec and sea/seb...
November 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29091956/genotypic-and-phenotypic-%C3%AE-lactam-resistance-and-presence-of-pvl-gene-in-staphylococci-from-dry-bovine-udder
#14
Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis Kollannur, Radhika Syam
Dairy cows affected with subclinical mastitis can be sources of virulent, antimicrobial-resistant Staphylococci to humans because of the excretion of the bacteria through their milk. This study focussed on the phenotypic and genotypic antibiotic resistance patterns of Staphylococci isolated from dairy cows in early dry period. Among 96 isolates of Gram positive cocci from 157 cows, 76 were identified as Coagulase Negative Staphylococci and the remaining 20 were Staphylococcus aureus. Typical amplicons of coagulase gene were obtained for all 20 samples of S...
2017: PloS One
https://www.readbyqxmd.com/read/29068720/detection-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-table-eggs-in-haripur-pakistan
#15
Muhammad Ali Syed, Syeda Hala Hussain Shah, Yasmin Sherafzal, Syed Shafi-Ur-Rehman, Mushtaq Ahmed Khan, John B Barrett, Tiffanie A Woodley, Bushra Jamil, Shahid Ahmad Abbasi, Charlene R Jackson
Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria monocytogenes, and Yersinia enterocolitica have already been reported from table eggs. More important is the presence of antimicrobial-resistant bacterial strains in this food source...
February 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29023054/genetic-characterization-antimicrobial-resistance-patterns-and-virulence-determinants-of-staphylococcus-aureus-isolated-form-bovine-mastitis
#16
Amal Awad, Hazem Ramadan, Sherif Nasr, Ahmed Ateya, Samar Atwa
BACKGROUND AND OBJECTIVE: Staphylococcus aureus is commonly associated with mastitis in dairy herds with potential public health implications. This study was conducted to investigate the existence of S. aureus in mastitic milk and to determine the antimicrobial resistance profiles of the isolated strains as well as the resistance and virulence associated genes. MATERIALS AND METHODS: Two hundred quarter milk samples were collected from 3 dairy farms at Dakahliya (n = 2) and Damietta (n = 1) Governorates, Egypt from September to December 2016...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/28941908/prevalence-of-staphylococcus-aureus-and-of-methicillin-resistant-s-%C3%A2-aureus-mrsa-along-the-production-chain-of-dairy-products-in-north-western-greece
#17
Panagiotis Papadopoulos, Theofilos Papadopoulos, Apostolos S Angelidis, Evridiki Boukouvala, Antonios Zdragas, Anna Papa, Christos Hadjichristodoulou, Daniel Sergelidis
The objective of this study was to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the production chain of dairy products. Of 367 tested samples (36 bulk tank milk (BTM), 19 dairy products, 72 human, 185 animal, 55 equipment), 212 (57.8%) were found positive for S. aureus. Almost all isolates (99.6%) were resistant to at least one antimicrobial and 13.3% were multi-drug resistant (MDR), exhibiting resistance to three or more antibiotic classes. Eleven samples (3%) were found contaminated by MRSA carrying the mecA gene...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28892893/distribution-of-sccmec-elements-and-presence-of-panton-valentine-leukocidin-in-methicillin-resistant-staphylococcusepidermidis-isolated-from-clinical-samples-in-a-university-hospital-of-isfahan-city-iran
#18
Mehrdad Halaji, Ashkan Karimi, Parisa Shoaei, Mohammadreza Nahaei, Farzin Khorvash, Behrooz Ataei, Majid Yaran, Seyed Asghar Havaei
INTRODUCTION: Coagulase Negative Staphylococcus (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients. AIM: The aim of this study was to identify the types of Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin (PVL) gene among clinical Methicillin-Resistant S. epidermidis (MRSE) isolates collected from Isfahan. MATERIALS AND METHODS: This cross-sectional study was performed from March 2014 to January 2015 at a tertiary care hospital of Isfahan, Iran...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28886942/remitting-infections-due-to-community-acquired-panton-valentine-leukocidin-producing-staphylococcus-aureus-in-the-milan-area
#19
Sara G Rimoldi, Cristina Pagani, Erika Longhi, Valentina D Cristo, Annamaria D Gregorio, Alessandro Mancon, Pietro Zerbi, Cristina Gervasoni, Maria R Gismondo, Agostino Riva
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations were performed to confirm the presence of the mecA and PVL genes and to assign the SCCmec type, sequence type (ST) and clonal relatedness. A strain of PVL-producing methicillin-resistant S. aureus (MRSA) was responsible for infection in two related families (A and B), and a third family (C) was infected with PVL-producing methicillin-sensitive S...
March 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28869541/spread-of-tst-positive-staphylococcus-aureus-strains-belonging-to-st30-clone-among-patients-and-healthcare-workers-in-two-intensive-care-units
#20
Matthaios Papadimitriou-Olivgeris, Eleanna Drougka, Fotini Fligou, Vasiliki Dodou, Fevronia Kolonitsiou, Kriton S Filos, Evangelos D Anastassiou, Efthimia Petinaki, Markos Marangos, Iris Spiliopoulou
Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton-Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton-Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR...
September 4, 2017: Toxins
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