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https://www.readbyqxmd.com/read/27899074/antibiotic-resistance-and-clonal-diversity-of-invasive-staphylococcus-aureus-in-the-rural-ashanti-region-ghana
#1
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May
BACKGROUND: Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. METHODS: Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S...
November 29, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27790966/epidemiology-and-microbiological-characterization-of-clinical-isolates-of-staphylococcus-aureus-in-a-single-healthcare-region-of-the-uk-2015
#2
C Horner, L Utsi, L Coole, M Denton
We investigated the epidemiology and characterization of isolates of Staphylococcus aureus within the Yorkshire and Humber (YH) region in the UK. In July 2015, each laboratory within YH (n = 14) was assigned two consecutive days during which all clinical isolates of S. aureus were collected. Isolates were tested for antibiotic susceptibilities and the presence of genes encoding methicillin resistance (mecA and mecC), Panton-Valentine leukocidin (PVL) (lukS-PV), and efflux-mediated chlorhexidine resistance (qacA); isolates were also characterized by spa-types...
October 28, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27669285/prevalence-of-methicillin-resistant-staphylococcus-aureus-and-other-staphylococcus-species-in-raw-meat-samples-intended-for-human-consumption-in-benin-city-nigeria-implications-for-public-health
#3
Etinosa O Igbinosa, Abeni Beshiru, Lucy U Akporehe, Faith E Oviasogie, Owen O Igbinosa
The present study was designed to characterize methicillin-resistant staphylococci from raw meat. A total of 126 meat samples were obtained from open markets between February and April, 2015. Antimicrobial susceptibility testing was carried out using the disc diffusion method. Molecular profiling was conducted using 16S rRNA, mecA, nuc, and PVL gene signatures were detected by polymerase chain reaction assay. Fifty isolates of methicillin-resistant Staphylococcus spp. were detected in 26 (52%) pork, 14 (28%) beef and 10 (20%) chicken samples...
September 24, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27603123/prevalence-and-characterization-of-oxacillin-susceptible-meca-positive-clinical-isolates-of-staphylococcus-aureus-causing-bovine-mastitis-in-india
#4
Hiral Mistry, Paresh Sharma, Sudipta Mahato, R Saravanan, P Anand Kumar, Vasundhra Bhandari
Bovine mastitis caused by multidrug resistant Staphylococcus aureus is a huge problem reported worldwide, resulting in prolonged antibiotic treatment and death of livestock. The current study is focused on surveillance of antibiotic susceptibility along with genotypic and phenotypic characterization of the pathogenic S. aureus strains causing mastitis in India. One hundred and sixty seven milk samples were collected from mastitis-affected cows from different farms in India resulting in thirty nine isolated S...
2016: PloS One
https://www.readbyqxmd.com/read/27553495/molecular-epidemiology-of-staphylococcus-aureus-from-lambar%C3%A3-n%C3%A3-gabon
#5
K V Okuda, N Toepfner, A S Alabi, B Arnold, S Bélard, U Falke, L Menschner, S Monecke, A Ruppelt-Lorz, R Berner
While there is an abundance of data on the epidemiology and molecular typing of Staphylococcus aureus, especially those carrying Panton-Valentine leucocidin (PVL) genes or mecA from Western Europe, Northern America and Australia, comparably few studies target African strains. In this study, we characterised genes associated with virulence and resistance, as well the phylogenetic background of S. aureus from healthy carriers and outpatients in Gabon. In total, 103 isolates from 96 study participants were characterised...
August 23, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27526771/prevalence-of-nasal-colonisation-by-methicillin-sensitive-and-methicillin-resistant-staphylococcus-aureus-among-healthcare-workers-and-students-in-madagascar
#6
Benedikt Hogan, Raphael Rakotozandrindrainy, Hassan Al-Emran, Denise Dekker, Andreas Hahn, Anna Jaeger, Sven Poppert, Hagen Frickmann, Ralf Matthias Hagen, Volker Micheel, Sabine Crusius, Jean Noel Heriniaina, Jean Philibert Rakotondrainiarivelo, Tsiriniaina Razafindrabe, Jürgen May, Norbert Georg Schwarz
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about the prevalence of S. aureus and MRSA among healthcare workers in Madagascar. The objective of our study was to examine the prevalence and clonal epidemiology of nasal S. aureus and MRSA among healthcare workers and non-medical University students in Antananarivo, Madagascar...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27432095/infective-endocarditis-caused-by-panton-valentine-leukocidin-producing-methicillin-susceptible-staphylococcus-aureus-identified-by-the-broad-range-pcr-method
#7
Hiroshi Nakajima, Kaoru Dohi, Masaki Tanabe, Akiko Nakamura, Shinji Kanemitsu, Hideo Wada, Norikazu Yamada, Tsutomu Nobori, Hideto Shinpo, Masaaki Ito
A 76-year-old man was admitted to a community hospital due to a persistent high fever. He became afebrile after the administration of broad-spectrum antibiotics, but developed heart failure due to progressive aortic and mitral valve insufficiency and was transferred to our hospital. Although sequential blood cultures were negative, a broad-range polymerase chain reaction targeting the bacterial 16S-rRNA gene followed by the direct sequencing of whole blood revealed spa(+), mecA(-) and Panton-Valentine leukocidin (PVL)(+)...
2016: Internal Medicine
https://www.readbyqxmd.com/read/27423957/short-communication-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-raw-milk-fresh-cheese-in-colombia
#8
Fanny C Herrera, María-Luisa García-López, Jesús A Santos
The aim of this study was the characterization of a collection of 8 methicillin-resistant Staphylococcus aureus (MRSA) isolates, obtained from samples of fresh cheese (Doble Crema) produced from raw cow milk in small dairies in Colombia. All the isolates harbored the mecA and Panton-Valentine leukocidin (PVL) genes, presented with SCCmec type IV, and belonged to multilocus sequence type 8 and spa type 024. Seven isolates presented 3 closely related pulsed-field gel electrophoresis profiles. Three of them carried the staphylococcal enterotoxin B gene...
October 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27398062/molecular-and-phenotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolates-from-a-tertiary-hospital-in-the-philippines
#9
Demetrio L Valle, Phyllis Anne P Paclibare, Esperanza C Cabrera, Windell L Rivera
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to public health worldwide. There are relatively few studies addressing the molecular epidemiology of MRSA in the Philippines. METHODS: This study characterized MRSA isolates in terms of their antimicrobial susceptibility profile, the SCCmec type, and the presence of lukF-lukS genes for Panton-Valentine leukocidin (PVL) and determined the relatedness of the isolates by random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR)...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27374894/biofilm-production-among-methicillin-resistant-staphylococcus-aureus-strains-isolated-from-catheterized-patients-with-urinary-tract-infection
#10
Fateh Rahimi, Mohammad Katouli, Sharmin Karimi
Between June 2011 and May 2014, we isolated a total of 419 Staphylococcus aureus strains from catheterized patients with UTI in a referral hospital in Tehran. Of these, 108 were identified as methicillin resistant (MRSA) based on their phenotypic resistance to oxacillin and the presence of mecA gene. The MRSA isolates were tested for their clonality using a combination of PFGE, prophage typing, SCCmec and ccr typing and examined for their biofilm formation as well as their resistance against 17 antibiotics...
September 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27326943/staphylococcus-aureus-carriage-in-selected-kindergartens-in-klang-valley
#11
N A Mohamed, S Ramli, N N Z Amin, W S W Sulaiman, I Isahak, T Z M T Jamaluddin, N M Salleh
INTRODUCTION: Nasal colonisation of S. aureus in healthy children was 18% to 30%. One to three percent of them were colonised by Methicillin-resistant Staphlycoccus aureus (MRSA). Although MRSA infection has become increasingly reported, population-based S. aureus and MRSA colonisation estimates are lacking. The main objective of this study was to determine the prevalence of S. aureus carriage among children. METHODS: Nasal samples for S. aureus culture were obtained from 250 children from three kindergartens in the Klang Valley, after consent was obtained from the children and their parents...
April 2016: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/27295910/prevalence-of-panton-valentine-leukocidin-genes-in-community-associated-methicillin-resistant-staphylococcus-aureus-in-the-district-of-pomoravlje
#12
Ljiljana Petrović Jeremić, Nada Kuljić Kapulica, Elizabeta Ristanović, Dragana Josić, Zorica Lepsanović
BACKGROUND/AIM: Community-associated methicillin-resistant Staphjlococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced virulence of CA-MRSA. The aim of this study was to detect the genes encoding PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje...
March 2016: Vojnosanitetski Pregled. Military-medical and Pharmaceutical Review
https://www.readbyqxmd.com/read/27262852/molecular-characterization-of-staphylococcus-aureus-isolates-causing-skin-and-soft-tissue-infections-in-patients-from-malakand-pakistan
#13
S Madzgalla, M A Syed, M A Khan, S S Rehman, E Müller, A Reissig, R Ehricht, S Monecke
Comparatively few studies have been published describing Staphylococcus aureus/MRSA epidemiology in Central Asia including Pakistan. Here, we report the genotyping of Staphylococcus aureus strains (that include both methicillin-susceptible and methicillin-resistant Staphylococcus aureus) from community- and hospital-acquired skin and soft-tissue infections in a tertiary care hospital in the Malakand district of the Khyber Pakhtunkhwa Province of Pakistan. Forty-five isolates of Staphylococcus aureus were characterized by microarray hybridization...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27257489/drug-resistance-and-genetic-characteristics-of-clinical-isolates-of-staphylococci-in-myanmar-high-prevalence-of-pvl-among-methicillin-susceptible-staphylococcus-aureus-belonging-to-various-sequence-types
#14
M S Aung, H Zi, K M Nwe, W W Maw, M T Aung, W W Min, N Nyein, M Kawaguchiya, N Urushibara, A Sumi, N Kobayashi
Prevalence, drug resistance and genetic characteristics were analysed for a total of 128 clinical isolates of staphylococci obtained from a tertiary hospital in Myanmar. The dominant species were S. aureus (39%) and S. haemolyticus (35%), followed by S. epidermidis (6%) and S. saprophyticus (5%). The majority of S. haemolyticus isolates (71.1%) harboured mecA, showing high resistance rates to ampicillin, cephalosporins, erythromycin and levofloxacin, while methicillin-resistant S. aureus (MRSA) was only 8% (four isolates) among S...
March 2016: New Microbes and New Infections
https://www.readbyqxmd.com/read/27179682/association-of-panton-valentine-leukocidin-pvl-genes-with-methicillin-resistant-staphylococcus-aureus-mrsa-in-western-nepal-a-matter-of-concern-for-community-infections-a-hospital-based-prospective-study
#15
Dharm R Bhatta, Lina M Cavaco, Gopal Nath, Kush Kumar, Abhishek Gaur, Shishir Gokhale, Dwij R Bhatta
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) is a major human pathogen associated with nosocomial and community infections. Panton Valentine leukocidin (PVL) is considered one of the important virulence factors of S. aureus responsible for destruction of white blood cells, necrosis and apoptosis and as a marker of community acquired MRSA. This study was aimed to determine the prevalence of PVL genes among MRSA isolates and to check the reliability of PVL as marker of community acquired MRSA isolates from Western Nepal...
2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27140201/community-associated-staphylococcus-aureus-pneumonia-among-greek-children-epidemiology-molecular-characteristics-treatment-and-outcome
#16
A G Doudoulakakis, D Bouras, E Drougka, M Kazantzi, A Michos, A Charisiadou, I Spiliopoulou, E Lebessi, M Tsolia
Staphylococcus aureus is an infrequent cause of community-associated (CA-SA) pneumonia in children. The aim of this study was to evaluate the clinical, epidemiological, microbiological, and molecular characteristics of CA-SA pneumonia among children hospitalized in two large tertiary care referral centers during an 8-year period. Cases of CA-SA pneumonia admitted between 2007 and 2014 were retrospectively examined through medical record review. Molecular investigation was performed for available strains; mecA, Panton-Valentine leukocidin (PVL) (lukS-lukF-PV), and fibronectin binding protein A (fnbA) genes were detected by polymerase chain reaction (PCR)...
July 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27099685/staphylococcus-aureus-isolates-carrying-panton-valentine-leucocidin-genes-their-frequency-antimicrobial-patterns-and-association-with-infectious-disease-in-shahrekord-city-southwest-iran
#17
Laleh Shariati, Majid Validi, Ali Mohammad Hasheminia, Reza Ghasemikhah, Fariborz Kianpour, Ali Karimi, Mohammad Reza Nafisi, Mohammad Amin Tabatabaiefar
BACKGROUND: A diversity of virulence factors work together to create the pathogenicity of Staphylococcus aureus. These factors include cell surface components that promote adherence to surfaces as well as exoproteins such as Panton-Valentine leukocidin (PVL), encoded by the luk-PV genes, that invade or bypass the immune system and are toxic to the host, thereby enhancing the severity of infections caused by methicillin-resistant Staphylococcus aureus (MRSA). OBJECTIVES: The aim of this study was to determine the frequency of PVL-positive MRSA strains by real-time PCR and their antibiotic susceptibility patterns by phenotypic test...
January 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27033676/a-persistent-antimicrobial-resistance-pattern-and-limited-methicillin-resistance-associated-genotype-in-a-short-term-staphylococcus-aureus-carriage-isolated-from-a-student-population
#18
Norhidayah Mat Azis, Hui P Pung, Abdul R Abdul Rachman, Syafinaz Amin Nordin, Seri N E Sarchio, Zarizal Suhaili, Mohd N Mohd Desa
The aim of the present study was to assess and compare the antimicrobial susceptibility pattern against a panel of antibiotics and molecular and methicillin resistance-associated genotypes of 120 carriage S. aureus isolates previously isolated from a student population at two isolation events within a one-month interval. The antibiotic susceptibility of isolates was determined using the Kirby-Bauer disc-diffusion method (cefoxitin by Etest). The MRSA was screened using polymerase chain reaction for the presence of the mecA gene...
March 28, 2016: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/27010813/methicillin-resistant-staphylococcus-aureus-with-mecc-a-description-of-45-human-cases-in-southern-sweden
#19
A-K Lindgren, E Gustafsson, A C Petersson, E Melander
In 2011, a novel mecA gene homologue, mecC, was reported in isolates from both humans and dairy cattle. The epidemiology of mecC methicillin-resistant Staphylococcus aureus (MRSA) in humans is not yet well known. In this retrospective study, we present the epidemiology of human clinical cases with mecC MRSA detected in the southern part of Sweden during the period 2005-2014. A total of 45 patients with an isolate positive for mecC MRSA were included in the study. Twenty-six isolates were found before 2012 and were retrospectively tested for mecC...
June 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26983032/detection-of-panton-valentine-leukocidin-positive-methicillin-resistant-staphylococcus-aureus-nasal-carriage-among-egyptian-health-care-workers
#20
Enas Mamdouh Hefzy, Gamal Mohamedin Hassan, Fadwa Abd El Reheem
BACKGROUND: Transmission of methicillin-resistant Staphylococcus aureus (MRSA) among patients is linked mainly to health care personnel. The Panton-Valentine leukocidin (PVL) is a cytotoxin that causes leukocyte lysis. Virulence of pvl-positive-MRSA has been attributed to its ability to express PVL toxin. METHODS: Swabs for detection of nasal carriage of pvl-positive MRSA among health care personnel at Fayoum University Hospital, Fayoum, Egypt, were collected from 223 health care personnel including 70 doctors (31...
June 2016: Surgical Infections
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