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Gene pvl and Meca

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https://www.readbyqxmd.com/read/29124834/prevalence-and-characterization-of-staphylococcus-aureus-causing-community-acquired-skin-and-soft-tissue-infections-on-java-and-bali-indonesia
#1
Dewi Santosaningsih, Sanarto Santoso, Nanik Setijowati, Harun A Rasyid, Nyoman S Budayanti, Ketut Suata, Dicky B Widhyatmoko, Priyo B Purwono, Kuntaman Kuntaman, Damayanti Damayanti, Cita R S Prakoeswa, Mitchell Laurens, Josephine W I van Nierop, Geraldine L Nanninga, Neline Oudenes, Michelle de Regt, Susan V Snijders, Henri A Verbrugh, Juliëtte A Severin
OBJECTIVES: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. METHODS: S. aureus were cultured from anterior nares, throat, and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S...
November 10, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29099921/methicillin-resistant-staphylococcus-aureus-in-minas-frescal-cheese-evaluation-of-classic-enterotoxin-genes-antimicrobial-resistance-and-clonal-diversity
#2
Alice Gonçalves Martins Gonzalez, Leila Márcia Peres Marques, Marcel da Silva Amorim Gomes, JhonathanCampos do Couto Beltrão, Marcos Gabriel Pinheiro, Luciana Maria Ramires Esper, Geraldo Renato de Paula, Lenise Arneiro Teixeira, Fábio Aguiar-Alves
This study aimed to investigate classical enterotoxin (sea to see) and mecA genes, by polymerase chain reaction (PCR) and anitimicrobial susceptibility, by disc diffusion test of Staphylococcus aureus isolated from minas frescal cheese (MFC). All methicillin resistant S. aureus (MRSA) isolates were investigated for the presence of PVL genes and clonal diversity. Thirty-one S. aureus were isolated from four MFC samples. Seven (22.6%) S. aureus carried mecA gene and two of them carried enterotoxin genes seb/sec and sea/seb...
November 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29091956/genotypic-and-phenotypic-%C3%AE-lactam-resistance-and-presence-of-pvl-gene-in-staphylococci-from-dry-bovine-udder
#3
Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis Kollannur, Radhika Syam
Dairy cows affected with subclinical mastitis can be sources of virulent, antimicrobial-resistant Staphylococci to humans because of the excretion of the bacteria through their milk. This study focussed on the phenotypic and genotypic antibiotic resistance patterns of Staphylococci isolated from dairy cows in early dry period. Among 96 isolates of Gram positive cocci from 157 cows, 76 were identified as Coagulase Negative Staphylococci and the remaining 20 were Staphylococcus aureus. Typical amplicons of coagulase gene were obtained for all 20 samples of S...
2017: PloS One
https://www.readbyqxmd.com/read/29068720/detection-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-table-eggs-in-haripur-pakistan
#4
Muhammad Ali Syed, Syeda Hala Hussain Shah, Yasmin Sherafzal, Syed Shafi-Ur-Rehman, Mushtaq Ahmed Khan, John B Barrett, Tiffanie A Woodley, Bushra Jamil, Shahid Ahmad Abbasi, Charlene R Jackson
Table eggs are nutritionally important food consumed globally. Despite being protected inside the hard shell and a semipermeable membrane, the egg contents may be contaminated with microbes and thus become a possible carrier of infectious agents to humans. A number of medically significant bacterial species such as Salmonella enterica, Listeria monocytogenes, and Yersinia enterocolitica have already been reported from table eggs. More important is the presence of antimicrobial-resistant bacterial strains in this food source...
October 25, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29023054/genetic-characterization-antimicrobial-resistance-patterns-and-virulence-determinants-of-staphylococcus-aureus-isolated-form-bovine-mastitis
#5
Amal Awad, Hazem Ramadan, Sherif Nasr, Ahmed Ateya, Samar Atwa
BACKGROUND AND OBJECTIVE: Staphylococcus aureus is commonly associated with mastitis in dairy herds with potential public health implications. This study was conducted to investigate the existence of S. aureus in mastitic milk and to determine the antimicrobial resistance profiles of the isolated strains as well as the resistance and virulence associated genes. MATERIALS AND METHODS: Two hundred quarter milk samples were collected from 3 dairy farms at Dakahliya (n = 2) and Damietta (n = 1) Governorates, Egypt from September to December 2016...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/28941908/prevalence-of-staphylococcus-aureus-and-of-methicillin-resistant-s-%C3%A2-aureus-mrsa-along-the-production-chain-of-dairy-products-in-north-western-greece
#6
Panagiotis Papadopoulos, Theofilos Papadopoulos, Apostolos S Angelidis, Evridiki Boukouvala, Antonios Zdragas, Anna Papa, Christos Hadjichristodoulou, Daniel Sergelidis
The objective of this study was to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the production chain of dairy products. Of 367 tested samples (36 bulk tank milk (BTM), 19 dairy products, 72 human, 185 animal, 55 equipment), 212 (57.8%) were found positive for S. aureus. Almost all isolates (99.6%) were resistant to at least one antimicrobial and 13.3% were multi-drug resistant (MDR), exhibiting resistance to three or more antibiotic classes. Eleven samples (3%) were found contaminated by MRSA carrying the mecA gene...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28892893/distribution-of-sccmec-elements-and-presence-of-panton-valentine-leukocidin-in-methicillin-resistant-staphylococcusepidermidis-isolated-from-clinical-samples-in-a-university-hospital-of-isfahan-city-iran
#7
Mehrdad Halaji, Ashkan Karimi, Parisa Shoaei, Mohammadreza Nahaei, Farzin Khorvash, Behrooz Ataei, Majid Yaran, Seyed Asghar Havaei
INTRODUCTION: Coagulase Negative Staphylococcus (CoNS) is considered as a major pathogen of nosocomial infections among immunosuppressed patients. AIM: The aim of this study was to identify the types of Staphylococcal Cassette Chromosome mec (SCCmec) and Panton-Valentine Leukocidin (PVL) gene among clinical Methicillin-Resistant S. epidermidis (MRSE) isolates collected from Isfahan. MATERIALS AND METHODS: This cross-sectional study was performed from March 2014 to January 2015 at a tertiary care hospital of Isfahan, Iran...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28886942/remitting-infections-due-to-community-acquired-panton-valentine-leukocidin-producing-staphylococcus-aureus-in-the-milan-area
#8
Sara Giordana Rimoldi, Cristina Pagani, Erika Longhi, Valentina Di Cristo, Annamaria Di Gregorio, Alessandro Mancon, Pietro Zerbi, Cristina Gervasoni, Maria Rita Gismondo, Agostino Riva
One of the most important Staphylococcus aureus virulence factors is Panton-Valentine leukocidin (PVL). We describe an outbreak of recurrent cutaneous PVL infections in different members of three family clusters. Molecular investigations were performed to confirm the presence of the mecA and PVL genes and to assign the SCCmec type, sequence type (ST) and clonal relatedness. A strain of PVL-producing methicillin-resistant S. aureus (MRSA) was responsible for infection in two related families (A and B), and a third family (C) was infected with PVL-producing methicillin-sensitive S...
September 5, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28869541/spread-of-tst-positive-staphylococcus-aureus-strains-belonging-to-st30-clone-among-patients-and-healthcare-workers-in-two-intensive-care-units
#9
Matthaios Papadimitriou-Olivgeris, Eleanna Drougka, Fotini Fligou, Vasiliki Dodou, Fevronia Kolonitsiou, Kriton S Filos, Evangelos D Anastassiou, Efthimia Petinaki, Markos Marangos, Iris Spiliopoulou
Staphylococcus aureus is a major cause of infections. Toxic shock syndrome toxin (TSST-1) and Panton-Valentine leukocidin (PVL) are associated with severe clinical syndromes. S. aureus colonizing isolates recovered from healthcare workers and patients in the intensive care unit (ICU) of a university hospital comprising Group A were compared with those from adult non-ICU carriers (Group B). mecA, lukS/lukF-PV (Panton-Valentine leukocidin, PVL), and tst (toxic shock syndrome toxin) gene carriage was detected by PCR...
September 4, 2017: Toxins
https://www.readbyqxmd.com/read/28865851/prevalence-antimicrobial-susceptibility-and-molecular-characterization-of-staphylococcus-aureus-isolated-from-dairy-herds-in-northern-china
#10
Huimin Liu, Songli Li, Lu Meng, Lei Dong, Shengguo Zhao, Xinyi Lan, Jiaqi Wang, Nan Zheng
Staphylococcus aureus is one of the main pathogens involved in dairy cow mastitis. Monitoring of antibiotic use would prove useful to assess the risk of Staph. aureus in raw milk. The objective of this work was to investigate the prevalence of Staph. aureus strais isolated from raw milk in northern China, and to characterize antimicrobial susceptibility of these strains and their key virulence genes. In total, 195 raw milk samples were collected from 195 dairy farms located in 4 cities of northern China from May to September 2015...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28859499/prevalence-and-molecular-typing-of-methicillin-resistant-staphylococcus-aureus-carrying-panton-valentine-leukocidin-gene
#11
Maryam Rahimpour Hesari, Ali Salehzadeh, Reza Kazemi Darsanaki
Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals...
September 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28828344/detection-of-panton-valentine-leukocidin-gene-isoforms-of-staphylococcus-aureus-isolates-in-al-zahra-hospital-isfahan-iran
#12
Seyed Asghar Havaei, Farkhondeh Poursina, Maryam Ahmadpour, Seyed Roholla Havaei, Meisam Ruzbahani
BACKGROUND: Panton-Valentine leukocidin (PVL) is a gamma-toxin produced by Staphylococcus aureus encoded by genes lukS/lukF-PV with several single-nucleotide polymorphisms. A mutation at nucleotide position 527 results in substitution of histidine (H) to arginine (R) at amino acid 176. The groups defined based on the amino acid change, the "R isoform" group and the "H isoform" group. The purpose of this study was to determine the frequency of PVL gene isoforms in S. aureus strains isolated from patients at Al-Zahra Hospital Isfahan and molecular characterization of PVL-positive methicillin-resistant S...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28757008/high-occurrence-of-mecc-mrsa-in-wild-hedgehogs-erinaceus-europaeus-in-sweden
#13
Björn Bengtsson, Lotta Persson, Kerstin Ekström, Helle Ericsson Unnerstad, Henrik Uhlhorn, Stefan Börjesson
We investigated the occurrence of mecC-MRSA in wild hedgehogs (Erinaceus europaeus) in Sweden and characterized the obtained isolates. Samples from 55 hedgehogs from five counties of Sweden were cultivated selectively for MRSA and putative isolates were confirmed by real-time PCR detecting mecA, mecC, nuc and PVL genes. mecC-MRSA was confirmed in 35 (64%) animals from three geographically separated counties. Confirmed isolates were spa-typed and tested for antimicrobial susceptibility by broth microdilution...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28729204/characterisation-of-clinical-isolates-of-oxacillin-susceptible-meca-positive-staphylococcus-aureus-in-china-from-2009-to-2014
#14
Yunjia Song, Lanqing Cui, Yuan Lv, Yun Li, Feng Xue
OBJECTIVES: The aim of this study was to investigate the prevalence and molecular characteristics of clinical oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) isolates in China from July 2009 to June 2014. METHODS: A total of 2068 non-duplicate S. aureus isolates were collected from 21 hospitals. Antimicrobial susceptibility testing was performed by the agar dilution method. All OS-MRSA strains were screened for the presence of the genes mecA, mecC and nuc as well as the Panton-Valentine leukocidin gene (pvl)...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28717309/molecular-characterization-of-antibiotic-resistant-staphylococcus-aureus-from-livestock-bovine-and-swine
#15
Asima Zehra, Randhir Singh, Simranpreet Kaur, J P S Gill
AIM: The aim of this study was to figure the prevalence, phenotypic and genotypic antibiotic resistance (AR) pattern of Staphylococcus aureus isolated from bovine and swine nares. MATERIALS AND METHODS: Colonies with typical morphology on Baird-Parker agar supplemented with egg-yolk tellurite emulsion were selected and biochemically/genotypically identified as S. aureus. These strains were further subjected to epsilometer test for their sensitivity to various clinically important antibiotics and antibiotic susceptibility testing for amoxicillin/clavulanic acid, and double-disk diffusion testing was performed by the standard disc diffusion method following CLSI guidelines...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28610560/prevalence-and-molecular-characteristics-of-staphylococcus-aureus-including-methicillin-resistant-strains-isolated-from-bulk-can-milk-and-raw-milk-products-in-pastoral-communities-of-south-west-uganda
#16
Benon B Asiimwe, Rossella Baldan, Alberto Trovato, Daniela M Cirillo
BACKGROUND: Staphylococcus aureus strains are now regarded as zoonotic agents. In pastoral settings where human-animal interaction is intimate, multi-drug resistant microorganisms have become an emerging zoonotic issue of public health concern. The study of S. aureus prevalence, antimicrobial resistance and clonal lineages in humans, animals and food in African settings has great relevance, taking into consideration the high diversity of ethnicities, cultures and food habits that determine the lifestyle of the people...
June 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28600770/real-time-pcr-to-identify-staphylococci-and-assay-for-virulence-from-blood
#17
Charles E Okolie
The genus Staphylococcus includes pathogenic and non-pathogenic facultative anaerobes. Due to the plethora of virulence factors encoded in its genome, the species Staphylococcus aureus is known to be the most pathogenic. S. aureus strains harboring genes encoding virulence and antibiotic resistance are of public health importance. In clinical samples, however, pathogenic S. aureus is often mixed with putatively less pathogenic coagulase-negative staphylococci (CoNS), both of which can harbor mecA, the genetic driver for staphylococcal methicillin-resistance...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28525941/environmental-contamination-with-staphylococcus-aureus-at-a-large-midwestern-university-campus
#18
Dipendra Thapaliya, Mohammed Taha, Mark R Dalman, Jhalka Kadariya, Tara C Smith
BACKGROUND: Staphylococcus aureus can cause minor to severe life-threatening infections. The changing epidemiology of S. aureus is of public health concern due to the emergence of multi-drug resistant (MDR) strains. Environmental surfaces play a crucial role in the transmission of S. aureus. The objective of this study was to examine environmental contamination and molecular characteristics of S. aureus in health professional-associated (HPA) and non-health professional-associated (NHPA) buildings at a large university...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28509842/molecular-epidemiology-of-methicillin-resistant-and-methicillin-susceptible-staphylococcus-aureus-in-the-ovine-dairy-chain-and-in-farm-related-humans
#19
Guerrino Macori, Giuseppina Giacinti, Alberto Bellio, Silvia Gallina, Daniela Manila Bianchi, Daniele Sagrafoli, Nicla Marri, Gilberto Giangolini, Simonetta Amatiste, Lucia Decastelli
Staphylococcus aureus is a major cause of clinical infections in humans and its enterotoxins cause foodborne disease. In the present study, we tested a total of 51 isolates of S. aureus from small-ruminant dairy farms with artisan dairy facilities, all located in Latium, Italy. The farms have a known history of a high prevalence of methicillin-resistant S. aureus (MRSA). Most of the MRSA isolates (27 of 51) belonged to spa-type t127 (43.1%), followed by t2678 (3.9%), t044 (2%), t1166 (2%), and t1773 (2%). PFGE performed on mecA positive strains identified one cluster (≥ 80% of similarity), comprising 22 MRSA...
May 16, 2017: Toxins
https://www.readbyqxmd.com/read/28504494/staphylococcus-aureus-nasal-carriage-and-its-antibiotic-resistance-profiles-in-children-in-high-altitude-areas-of-southwestern-china
#20
Zongrong Gong, Min Shu, Qing Xia, Shan Tan, Wei Zhou, Yu Zhu, Chaomin Wan
BACKGROUND/AIM: To describe the epidemiological profile of nasal carriage of Staphylococcus aureus (S. aureus) strains, its antibiotic resistance and mecA and Panton Valentine leukocidin (PVL) genes presence, in school children residing in high altitude areas of Southwestern China. METHODS: The cross sectional study screened nasal swabs taken from students for S.aureus. PCR was performed to identify mecA and PVL genes. RESULTS: Of the total 314 children 5...
June 1, 2017: Archivos Argentinos de Pediatría
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