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MMP-2, MMP-9

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https://www.readbyqxmd.com/read/28440509/modulation-of-mmp-2-and-9-secretion-by-cytokines-inducers-and-inhibitors-in-human-melanoma-a-2058-cells
#1
M Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki, Matthias Rath
Melanoma, an extremely aggressive cancer, causes the most skin cancer-related deaths, due to metastasis to other areas of the body, such as lymph nodes, lungs, liver, brain or bone. It is characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the extracellular matrix and basement membrane, allowing cancer cells to spread to distal organs. Various cytokines, mitogens, growth factors, inducers and inhibitors control MMP activities. We investigated the roles of these in regulation of MMP-2 and -9 in human melanoma A-2058 cells...
April 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28440462/imperatorin-efficiently-blocks-tnf-%C3%AE-mediated-activation-of-ros-pi3k-akt-nf-%C3%AE%C2%BAb-pathway
#2
Ke Si Wang, Ying Lv, Zhe Wang, Juan Ma, Chunliu Mi, Xuezheng Li, Guang Hua Xu, Lian Xun Piao, Sheng Zhe Zheng, Xuejun Jin
Inflammation contributes to development and progression in a variety of cancers, including cervical cancer, which is the second leading cause of cancer deaths in women worldwide. In this study, we examined the anti-inflammatory effects of imperatorin, a psoralen-type furanocoumarin from the fruits of Angelica dahurica, in tumor necrosis factor-α (TNF-α)-stimulated HeLa cells by investigating its impact on the production and expression of cytokines and the major signal-transduction pathways. We found this compound significantly inhibited the TNF-α-induced expression of NF-κB target genes, such as COX-2, cyclin  D1, MMP-9, VEGF, IL-6 and Bcl-xL in a concentration-dependent manner...
April 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28435396/lentivirus-mediated-knockdown-of-p27rf-rho-inhibits-hepatocellular-carcinoma-cell-growth
#3
Shuli Xie, Guangyi Wang, Guofu Chen, Mingguang Zhu, Guoyue Lv
AIM OF THE STUDY: To investigate the effects of P27RF-Rho on hepatocellular carcinoma (HCC) cell growth and explore the possibility of using it as a novel therapeutic target for liver cancer treatment. MATERIAL AND METHODS: P27RF-Rho in HCC cells was silenced by lentivirus-mediated RNA interference, and the silencing effect was verified by RT-PCR. Cell proliferation was determined by MTT and clone formation assay. Cell cycle phase and apoptosis were detected through FACS...
2017: Contemporary Oncology Współczesna Onkologia
https://www.readbyqxmd.com/read/28435292/reg3a-promotes-the-proliferation-migration-and-invasion-of-gastric-cancer-cells
#4
Zhou-Feng Chen, Zhi-Ming Huang, Hai-Bo Xue, Xiu-Qing Lin, Ren-Ping Chen, Meng-Jun Chen, Rui-Fang Jin
The mechanism underlying the metastasis of gastric cancer (GC) cells remains elusive. REG3A is considered an oncogene in various cancers, but in GC its role is unclear. Here, we report that the expression of REG3A was significantly increased in the tumor tissues of patients with GC compared with the matched normal tissues. Knockdown of REG3A induced by specific small interfering RNA (siRNA) significantly repressed the proliferation of GC cells for 24 h or 48 h. Moreover, knockdown of REG3A significantly suppressed the migration, invasion, and adhesion of GC cells in vitro...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28435247/delivery-of-curcumin-by-directed-self-assembled-micelles-enhances-therapeutic-treatment-of-non-small-cell-lung-cancer
#5
Wen-Ting Zhu, Sheng-Yao Liu, Lei Wu, Hua-Li Xu, Jun Wang, Guo-Xin Ni, Qing-Bing Zeng
BACKGROUND: It has been widely reported that curcumin (CUR) exhibits anticancer activity and triggers the apoptosis of human A549 non-small-cell lung cancer (NSCLC) cells. However, its application is limited owing to its poor solubility and bioavailability. Therefore, there is an urgent need to develop a new CUR formulation with higher water solubility and better biocompatibility for clinical application in the future. MATERIALS AND METHODS: In this study, CUR-loaded methoxy polyethylene glycol-polylactide (CUR/mPEG-PLA) polymeric micelles were prepared by a thin-film hydration method...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28435089/cobalt-chloride-induces-rhoa-rock-activation-and-remodeling-effect-in-h9c2-cardiomyoblasts-involvement-of-pi3k-akt-and-mapk-pathways
#6
Cheng-I Cheng, Yueh-Hong Lee, Po-Han Chen, Yu-Chun Lin, Ming-Huei Chou, Ying-Hsien Kao
Chronic heart failure is a serious complication of myocardial infarction, one of the major causes of death worldwide that often leads to adverse cardiac hypertrophy and poor prognosis. Hypoxia-induced cardiac tissue remodeling is considered an important underlying etiology. This study aimed to delineate the signaling profile of RhoA/ROCK, PI3K/Akt, and MAPK and their involvement in regulation of remodeling events in cultured H9c2 cardiomyoblast cells. In addition to its growth-suppressive effect, the hypoxia-mimetic chemical, cobalt chloride (CoCl2) significantly induced RhoA kinase activation as revealed by increased MBS phosphorylation and ROCK1/2 expression in H9c2 cells...
April 20, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28435016/the-angiostatic-molecule-multimerin-2-is-processed-by-mmp-9-to-allow-sprouting-angiogenesis
#7
Eva Andreuzzi, Roberta Colladel, Rosanna Pellicani, Giulia Tarticchio, Renato Cannizzaro, Paola Spessotto, Benedetta Bussolati, Alessia Brossa, Paolo De Paoli, Vincenzo Canzonieri, Renato V Iozzo, Alfonso Colombatti, Maurizio Mongiat
Angiogenesis is a crucial process occurring under physiological and pathological conditions, including cancer. The development of blood vessels is tightly regulated by a plethora of cytokines, endothelial cell (EC) receptors and extracellular matrix (ECM) components. In this context, we have shown that Multimerin 2 (MMRN2), an ECM molecule specifically secreted by ECs, exerts angiostatic functions by binding VEGFA and other pro-angiogenic cytokines. Here, we demonstrate that during angiogenic stimuli MMRN2 mRNA levels significantly decrease...
April 20, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28433630/loss-of-murc-cavin-4-induces-jnk-and-mmp-9-activity-enhancement-in-vascular-smooth-muscle-cells-and-exacerbates-abdominal-aortic-aneurysm
#8
Kotaro Miyagawa, Takehiro Ogata, Tomomi Ueyama, Takeru Kasahara, Naohiko Nakanishi, Daisuke Naito, Takuya Taniguchi, Tetsuro Hamaoka, Naoki Maruyama, Masahiro Nishi, Taizo Kimura, Hiroyuki Yamada, Hiroki Aoki, Satoaki Matoba
Abdominal aortic aneurysm (AAA) is relatively common in elderly patients with atherosclerosis. MURC (muscle-restricted coiled-coil protein)/Cavin-4 modulating the caveolae function of muscle cells is expressed in cardiomyocytes, skeletal muscle cells and smooth muscle cells. Here, we show a novel functional role of MURC/Cavin-4 in vascular smooth muscle cells (VSMCs) and AAA development. Both wild-type (WT) and MURC/Cavin-4 knockout (MURC(-/-)) mice subjected to periaortic application of CaCl2 developed AAAs...
April 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28432277/transforming-growth-factor-%C3%AE-1-promotes-invasion-of-human-jeg-3-trophoblast-cells-via-tgf-%C3%AE-smad3-signaling-pathway
#9
Zhongying Huang, Shangwei Li, Wei Fan, Qianhong Ma
Transforming growth factor (TGF)-β1 is involved invasion of human trophoblasts. However, the underlying mechanisms remain unclear. In this study, we performed Transwell assay and found that TGF-β1 promoted the invasion of trophoblast cell line JEG-3. Treatment with TGF-β1 up-regulated the expression of receptor-regulated Smad transcription factors Smad2 and Smad3, and two invasive-associated genes, namely, matrix metallopeptidase (MMP)-9 and MMP-2, in JEG-3 cells. Over-expressing activin receptor-like kinase (ALK) 5, the TGF-β type I receptor (TβRI) enhanced the up-regulation of Smad2, Smad3, MMP-9, and MMP-2 induced by TGF-β1, whereas application of TβRI inhibitor SB431542 diminished the stimulatory effects of TGF-β1 on these genes...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28431514/associations-between-matrix-metalloproteinase-gene-polymorphisms-and-glaucoma-susceptibility-a-meta-analysis
#10
Ming-Yue Wu, Yang Wu, Yong Zhang, Cai-Yun Liu, Chun-Yan Deng, Le Peng, Lan Zhou
BACKGROUND: Matrix metalloproteinases (MMPs) polymorphisms have been implicated in the pathogenesis of glaucoma risk. However, the results were controversial. We performed a meta-analysis to evaluate the precise associations between MMPs polymorphisms and glaucoma risk. METHODS: Related studies were reviewed by searching electronic databases within four databases. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association between the most common polymorphisms of MMPs and glaucoma risk...
April 21, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28431065/cha2ds2-vasc-score-and-blood-biomarkers-to-identify-patients-with-atrial-high-rate-episodes-and-paroxysmal-atrial-fibrillation
#11
Paulina Wakula, Benjamin Neumann, Jens Kienemund, Eva Thon-Gutschi, Tatjana Stojakovic, Martin Manninger, Daniel Scherr, Hubert Scharnagl, Martin Kapl, Burkert Pieske, Frank R Heinzel
Aims: Paroxysmal atrial fibrillation (PAF) is often asymptomatic but nonetheless harmful. We evaluated the performance of disease-related blood biomarkers and CHA2DS2-VASc score to discriminate for PAF in patients with continuous rhythm monitoring. Methods and Results: Clinical data and blood samples were obtained from patients with dual-chamber pacemakers selected according to the absence (no_AHRE) or presence of Atrial High-Rate Episodes (AHRE) >6 min in recent device history (case-control approach)...
April 1, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28429540/silencing-of-astrocyte-elevated-gene-1-inhibits-proliferation-and-migration-and-induces-apoptosis-in-melanoma
#12
Yue Zhang, Ge Peng, Ying Wang, Lixia Cui, Wenqing Wu, Luan Wang, Chengyu Liu, Xiuping Han
Melanoma is an aggressive skin malignancy with a high mortality. Astrocyte elevated gene-1 (AEG-1), a downstream target of ras and c-myc, has been implicated in the development of multiple tumors, yet its role in melanoma remains unclear. In the present study, the role of AEG-1 in melanoma was explored through AEG-1 silencing. Our results showed that silencing of AEG-1 inhibited proliferation of melanoma cells and induced cell cycle arrest, accompanied by reduced levels of cyclinA, cyclinB, cyclinD1, cyclinE and cyclin dependent kinase 2...
April 21, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28426994/silica-nanoparticles-induced-intrinsic-apoptosis-in-neuroblastoma-sh-sy5y-cells-via-cytc-apaf-1-pathway
#13
Yanyan Yang, Yongbo Yu, Jiahui Wang, Yanbo Li, Yang Li, Jia Wei, Tong Zheng, Minghua Jin, Zhiwei Sun
The present study was to investigate effects of Silica nanoparticles (SiNPs) on nervous system and explore potential mechanisms in human neuroblastoma cells (SH-SY5Y). Cytotoxicity was detected by cell viability and Lactate dehydrogenase (LDH) release. Flow cytometry analysis was applied to assess mitochondrial membrane potential (MMP) loss, intracellular Ca(2+) and apoptosis. To clarify the mechanism of SiNPs-induced apoptosis, intrinsic apoptosis-related proteins were detected. Our results showed that SiNPs caused cytotoxicity, cell membrane damage and Ca(2+) increase in a dose-dependent manner in SH-SY5Y cells...
January 30, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28426786/t140-blocks-the-sdf-1-cxcr4-signaling-pathway-and-prevents-cartilage-degeneration-in-an-osteoarthritis-disease-model
#14
Kun Wang, Yanlin Li, Rui Han, Guofeng Cai, Chuan He, Guoliang Wang, Di Jia
Osteoarthritis (OA) is one of the most common diseases affecting older people; however, there remains no effective targeted drug to combat OA. The aims of this study were (1) to explore the effect of T140 in regulating degeneration of articular cartilage in vivo by targeted blocking of the SDF-1/CXCR4 signaling pathway, and (2) to provide experimental evidence for the development of a novel OA-targeted pharmacotherapy. Thirty-six healthy Hartley guinea pigs were randomly divided into three groups: a T140-treated group (n = 12), a phosphate buffer saline control group (n = 12) and an untreated control group (n = 12)...
2017: PloS One
https://www.readbyqxmd.com/read/28426715/acute-hypoxia-influences-collagen-and-matrix-metalloproteinase-expression-by-human-keratoconus-cells-in-vitro
#15
Tina B McKay, Jesper Hjortdal, Shrestha Priyadarsini, Dimitrios Karamichos
Keratoconus (KC) is a progressive corneal ectasia linked to thinning of the central cornea. Hard contact lenses, rigid gas permeable lenses, and scleral lenses are the primary treatment modalities for early to mid- stages of KC to correct refractive error and astigmatism that develops as a result of an irregular corneal structure. These treatments are associated with significant drawbacks, including reduced availability of the tear film and oxygen to the corneal epithelium and stroma. However, it remains unknown whether hypoxia affects corneal integrity in the KC pathobiology...
2017: PloS One
https://www.readbyqxmd.com/read/28424417/mesenchymal-stem-cells-differentially-affect-the-invasion-of-distinct-glioblastoma-cell-lines
#16
Barbara Breznik, Helena Motaln, Miloš Vittori, Ana Rotter, Tamara Lah Turnšek
Glioblastoma multiforme are an aggressive form of brain tumors that are characterized by distinct invasion of single glioblastoma cells, which infiltrate the brain parenchyma. This appears to be stimulated by the communication between cancer and stromal cells. Mesenchymal stem cells (MSCs) are part of the glioblastoma microenvironment, and their 'cross-talk' with glioblastoma cells is still poorly understood. Here, we examined the effects of bone marrow-derived MSCs on two different established glioblastoma cell lines U87 and U373...
March 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423707/tropomyosin-related-kinase-b-mediated-signaling-contributes-to-the-induction-of-malignant-phenotype-of-gallbladder-cancer
#17
Makoto Kawamoto, Hideya Onishi, Keigo Ozono, Akio Yamasaki, Akira Imaizumi, Sachiko Kamakura, Kenji Nakano, Yoshinao Oda, Hideki Sumimoto, Masafumi Nakamura
This study aims to demonstrate the clinical and biological significance of Brain derived neurotrophic factor (BDNF)/Tropomyosin-related kinase B (TrkB) signaling in gallbladder cancer (GBC) through a series of in vitro and in vivo experiments. TrkB expression was detected in 63 (91.3%) out of 69 surgically resected primary GBC specimens by immunohistochemistry. TrkB expression in the invasive front correlated with T factor (p=0.0391) and clinical staging (p=0.0391). Overall survival was lower in patients with high TrkB expression in the invasive front than in those with low TrkB expression (p=0...
March 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423649/stellettin-b-induces-apoptosis-in-human-chronic-myeloid-leukemia-cells-via-targeting-pi3k-and-stat5
#18
Yali Chen, Qianxiang Zhou, Lei Zhang, Yuxu Zhong, Guanwei Fan, Zhe Zhang, Ran Wang, Meihua Jin, Yuling Qiu, Dexin Kong
Novel agents are still urgently expected for therapy of chronic myeloid leukemia (CML). The in vitro anti-leukemia activity of Stellettin B (Stel B), a triterpenoid we isolated from marine sponge Jaspis stellifera, on human CML K562 and KU812 cells was recently investigated. Stel B inhibited K562 and KU812 cell proliferation with IC50 as 0.035 μM and 0.95 μM respectively. While no obvious cell cycle arrest was observed, apoptosis was induced in K562 cells after Stel B treatment. The Stel B-induced apoptosis might be in mitochondrial pathway, with increase of Bad and Bax, decrease of Bcl-2 and activation of caspase-9...
March 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421659/the-anti-inflammatory-effects-of-morin-hydrate-in-atherosclerosis-is-associated-with-autophagy-induction-through-camp-signaling
#19
Yue Zhou, Zhan-Qi Cao, Hong-Yuan Wang, Yan-Na Cheng, Lu-Gang Yu, Xin-Ke Zhang, Yan Sun, Xiu-Li Guo
SCOPE: Although the previous trials of inflammation have indicated that morin hydrate (MO) hold considerable promise, understanding the distinct mechanism of MO against inflammation remains a challenge. METHODS AND RESULTS: This study investigated the effect of MO in atherosclerosis in ApoE(-/-) mice and underlying cell signaling of MO effect in inflammation in human umbilical vein endothelial cells (HUVECs). Administration of MO significantly reduced serum lipid level, inflammatory cytokines (TNF-α and ICAM-1) and atherosclerotic plaque formation in vivo...
April 18, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28421528/postnatal-lps-challenge-impacts-escape-learning-and-expression-of-plasticity-factors-mmp9-and-timp1-in-rats-effects-of-repeated-training
#20
Alexander Trofimov, Tatyana Strekalova, Niall Mortimer, Olga Zubareva, Alexander Schwarz, Evgeniy Svirin, Aleksei Umriukhin, Andrei Svistunov, Klaus-Peter Lesch, Victor Klimenko
Bacterial intoxication associated with inflammatory conditions during development can impair brain functions, in particular evolutionarily novel forms of memory, such as explicit learning. Little is known about the dangers of early-life inflammation on more basic forms of learning, for example, the acquisition of motor escape abilities, which are generally better preserved under pathological conditions. To address this limitation in knowledge, an inflammatory response was elicited in Wistar pups by lipopolysaccharide (LPS) injections (25 μg/kg) on postnatal days P15, P18 and P21...
April 18, 2017: Neurotoxicity Research
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