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https://www.readbyqxmd.com/read/28520093/verticillium-dahliae-transcription-factor-vdftf1-regulates-the-expression-of-multiple-secreted-virulence-factors-and-is-required-for-full-virulence-in-cotton
#1
Wen-Qi Zhang, Yue-Jing Gui, Dylan P G Short, Ting-Gang Li, Dan-Dan Zhang, Lei Zhou, Chun Liu, Yu-Ming Bao, Krishna V Subbarao, Jie-Yin Chen, Xiao-Feng Dai
Fungal transcription factors (TFs) implicated in the regulation of virulence gene expression have been identified in a number of plant pathogens. In Verticillium dahliae, despite its agricultural importance, few regulators of transcription have been characterized. In this study, a T-DNA insertional mutant with significantly reduced virulence toward cotton was identified. The T-DNA was traced to VdFTF1, a gene encoding a TF containing a Fungal_trans domain. Transient expression in onion epidermal cells indicated that VdFTF1 is localized to the nucleus...
May 18, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28506210/sucrose-non-ferment-1-related-protein-kinase-2-snrk2-genes-could-mediate-the-stress-responses-in-potato-solanum-tuberosum-l
#2
Jiangping Bai, Juan Mao, Hongyu Yang, Awais Khan, Aqi Fan, Siyan Liu, Junlian Zhang, Di Wang, Huijuan Gao, Jinlin Zhang
BACKGROUND: The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). RESULTS: In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L...
May 15, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28504277/role-of-dietary-onion-in-modifying-the-faecal-bile-acid-content-in-rats-fed-a-high-cholesterol-diet
#3
Diana González-Peña, Lucía Giménez, Begoña de Ancos, Concepción Sánchez-Moreno
The determination of faecal bile patterns offers new opportunities in the search for non-invasive biomarkers of disease status. The objective of this study was to describe the shifts in faecal bile acid (BA) composition induced by feeding a high-cholesterol/cholic acid diet (HC) over 7 weeks of experimental feeding in Wistar rats, and to evaluate the effect of onion included as a functional ingredient (HCO). A HPLC-MS/MS method allowed the detection of 29 bile acids, 10 of which were tentatively identified and 12 confirmed and quantified by means of standards and calibration curves...
May 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28503942/optimisation-of-steam-distillation-extraction-oil-from-onion-by-response-surface-methodology-and-its-chemical-composition
#4
Zhao Dan Wang, Li Hua Li, Hui Xia, Feng Wang, Li Gang Yang, Shao Kang Wang, Gui Ju Sun
Oil extraction from onion was performed by steam distillation. Response surface methodology was applied to evaluate the effects of ratio of water to raw material, extraction time, zymolysis temperature and distillation times on yield of onion oil. The maximum extraction yield (1.779%) was obtained as following conditions: ratio of water to raw material was 1, extraction time was 2.5 h, zymolysis temperature was 36° and distillation time was 2.6 h. The experimental values agreed well with those predicted by regression model...
May 15, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28501821/genetic-and-clinical-characteristics-of-nefl-related-charcot-marie-tooth-disease
#5
Alejandro Horga, Matilde Laurà, Zane Jaunmuktane, Nivedita U Jerath, Michael A Gonzalez, James M Polke, Roy Poh, Julian C Blake, Yo-Tsen Liu, Sarah Wiethoff, Conceição Bettencourt, Michael Pt Lunn, Hadi Manji, Michael G Hanna, Henry Houlden, Sebastian Brandner, Stephan Züchner, Michael Shy, Mary M Reilly
OBJECTIVES: To analyse and describe the clinical and genetic spectrum of Charcot-Marie-Tooth disease (CMT) caused by mutations in the neurofilament light polypeptide gene (NEFL). METHODS: Combined analysis of newly identified patients with NEFL-related CMT and all previously reported cases from the literature. RESULTS: Five new unrelated patients with CMT carrying the NEFL mutations P8R and N98S and the novel variant L311P were identified...
May 13, 2017: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/28495367/in-vivo-effects-of-allium-cepa-l-on-the-selected-gut-microflora-and-intestinal-histomorphology-in-broiler
#6
Sajid Ur Rahman, Sarzamin Khan, Naila Chand, Umar Sadique, Rifat Ullah Khan
The experiment was conducted to evaluate the effect of onion (Allium cepa L.,) on the performance, gut microflora and intestinal histomorphology of broiler chicks. Three hundred and twenty day old broiler chicks (Ross) were randomly divided into four groups as A, B, C and D. Group A served as control without any supplementation, group B, C and D was supplemented with onion powder at the rate of 1.5, 2 and 2.5g/kg of feed respectively. Supplementation of onion significantly (P<0.05) increased weight gain and feed consumption...
May 8, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28495047/similar-clinical-pathological-and-genetic-features-in-chinese-patients-with-autosomal-recessive-and-dominant-charcot-marie-tooth-disease-type-2k
#7
Jun Fu, Shixu Dai, Yuanyuan Lu, Rui Wu, Zhaoxia Wang, Yun Yuan, He Lv
Mutations in the ganglioside-induced differentiation-associated protein 1 gene (GDAP1) cause rare subtypes of Charcot-Marie-Tooth disease (CMT2K and CMT4A). CMT2K is an axonal neuropathy while CMT4A is a demyelinating type. In a series of 169 Chinese CMT patients (79 CMT1, 52 CMT2 and 38 unclassified), four unrelated patients (2.37%) were identified with GDAP1 mutations, including two with autosomal recessive CMT2K (AR-CMT2K) and two dominant CMT2K (AD-CMT2K). All patients had disease onset before 5 years of age, and presented with muscle weakness, atrophy, and mild sensory disturbance in distal limbs...
April 20, 2017: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28493683/plant-uptake-and-metabolism-of-nitrofuran-antibiotics-in-spring-onion-grown-in-nitrofuran-contaminated-soil
#8
Yinan Wang, K K Jason Chan, Wan Chan
Environmental pollution caused by the discharge of mutagenic and carcinogenic nitrofuran to the aquatic and soil environment is an emerging public health concern because of the potential in producing drug-resistant microbes and being uptaken by food crops. Using liquid chromatography-tandem mass spectrometry analysis and with spring onion (Allium wakegi Araki) as the plant model, we investigated in this study the plant uptake and accumulation of nitrofuran from contaminated environment. Our study revealed for the first time high uptake and accumulation rates of nitrofuran in the edible parts of the food crop...
May 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28475377/comparative-studies-of-bioactive-organosulphur-compounds-and-antioxidant-activities-in-garlic-allium-sativum-l-elephant-garlic-allium-ampeloprasum-l-and-onion-allium-cepa-l
#9
Sunyoung Kim, Dan-Bi Kim, Wenjie Jin, Junghyuck Park, Wonjin Yoon, Yunyeol Lee, Soyoung Kim, Sanghee Lee, Sungsoo Kim, Ok-Hwan Lee, Dongbin Shin, Miyoung Yoo
We evaluated organosulphur compounds in Allium vegetables, including garlic, elephant garlic and onion, using high-performance liquid chromatography. Among organosulphur compounds, elephant garlic had considerable γ-glutamyl peptides, and garlic had the highest alliin content. Onion had low level of organosulphur compounds than did elephant garlic and garlic. In addition, antioxidant capacities were evaluated by oxygen radical absorbance capacity (ORAC) values and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay...
May 5, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28471462/the-role-of-metals-in-mammalian-olfaction-of-low-molecular-weight-organosulfur-compounds
#10
REVIEW
Eric Block, Victor S Batista, Hiroaki Matsunami, Hanyi Zhuang, Lucky Ahmed
Covering: up to the end of 2017While suggestions concerning the possible role of metals in olfaction and taste date back 50 years, only recently has it been possible to confirm these proposals with experiments involving individual olfactory receptors (ORs). A detailed discussion of recent experimental results demonstrating the key role of metals in enhancing the response of human and other vertebrate ORs to specific odorants is presented against the backdrop of our knowledge of how the sense of smell functions both at the molecular and whole animal levels...
May 10, 2017: Natural Product Reports
https://www.readbyqxmd.com/read/28469451/pesticide-residues-in-commercial-lettuce-onion-and-potato-samples-from-bolivia-a-threat-to-public-health
#11
REVIEW
Marlene Skovgaard, Susana Renjel Encinas, Olaf Chresten Jensen, Jens Hinge Andersen, Guido Condarco, Erik Jørs
Bolivia does not have a surveillance program for pesticide residues in food. The few published studies have suggested that pesticide contamination in food may present a public health problem. Data are lacking for all foods except tomatoes and breast milk. In this study 10 potato, 10 onion, and 10 lettuce samples from La Paz were sampled on August 15, 2015 at a local market and screened for 283 pesticides. Residues of cypermethrin, chlorpyrifos, difenoconazol, or/and λ-cyhalothrin were detected in 50% of the lettuce samples, whereas no pesticides were found in potatoes and onions...
2017: Environmental Health Insights
https://www.readbyqxmd.com/read/28467185/copper-and-zinc-tolerance-in-bacteria-isolated-from-fresh-produce
#12
Ismael Cidre, Rubén Pérez Pulido, Maria José Grande Burgos, Antonio Gálvez, Rosario Lucas
The continued agricultural exposure of bacteria to metals such as copper and zinc may result in an increased copper tolerance through the food chain. The aim of this study was to determine the Cu and Zn tolerance of bacteria from fresh produce (cucumber, zucchini, green pepper, tomato, lettuce, vegetable salad, broccoli, cabbage, carrot, green onion, onion, and mango). Isolates (506 aerobic mesophiles) from 12 different food produce products were tested for growth in a range of Cu and Zn concentrations. Selected isolates were identified using 16S rDNA sequencing, and the presence of metal resistance genes was studied using PCR amplification...
May 3, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28465519/the-conidial-mucilage-natural-film-coatings-is-involved-in-environmental-adaptability-and-pathogenicity-of-hirsutella-satumaensis-aoki
#13
Jiaojiao Qu, Xiao Zou, Jianping Yu, Yeming Zhou
The Hirsutella genus is very special asexually-reproducing pathogens of insects by reduced sporulation, host specificity and spores covered by a thick mucilage layer. However, the ecological function of conidial mucilage remains elusive. In this study, the possible ecological role of conidial mucilage from the entomopathogenic fungus Hirsutella satumaensis was functionally investigated through tolerance, adherence and insect bioassays involving aerial conidia (AC) and mucilage-free conidia (MFC). Measurements of hydrophobicity using microbial adhesion to hydrocarbons (MATH) indicated that mucilage is main contributor to the surface hydrophobicity of AC...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28460970/3-4-dihydroxyphenylacetic-acid-is-a-predominant-biologically-active-catabolite-of-quercetin-glycosides
#14
Yue Tang, Sayaka Nakashima, Shunya Saiki, Yui Myoi, Naomi Abe, Shoko Kuwazuru, Beiwei Zhu, Hitoshi Ashida, Yoshiyuki Murata, Yoshimasa Nakamura
Since dietary flavonoid glycosides, including quercetin 4'-glucoside from onion, are poorly absorbed from the gastrointestinal tract, they are converted into smaller phenolic acids, which can be absorbed into the circulation. The purpose of this study was to compare the effects of the major phenolic acid catabolites of quercetin 4'-glucoside, including 3,4-dihydroxyphenylacetic acid (DOPAC), 3-hydroxyphenylacetic acid, 3,4-dihydroxybenzoic acid (protocatechuic acid) and hippuric acid, on the antioxidant activity and phase II cytoprotective enzyme induction in vitro...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28460952/compositional-evaluation-of-selected-agro-industrial-wastes-as-valuable-sources-for-the-recovery-of-complex-carbohydrates
#15
Aleksandra Vojvodić, Draženka Komes, Irena Vovk, Ana Belščak-Cvitanović, Arijana Bušić
Re-utilization of various agro-industrial wastes is of growing importance from many aspects. Considering the variety and complexity of such materials, compositional data and compliant methodology is still undergoing many updates and improvements. Present study evaluated sugar beet pulp (SBP), walnut shell (WS), cocoa bean husk (CBH), onion peel (OP) and pea pods (PP) as potentially valuable materials for carbohydrate recovery. Macrocomponent analyses revealed carbohydrate fraction as the most abundant, dominating in dietary fibres...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28459863/effect-of-processing-on-composition-changes-of-selected-spices
#16
Cai-Hua Jia, Jung-Ah Shin, Young-Min Kim, Ki-Teak Lee
The present investigation was conducted to study the true retentions of α-tocopherol, tocotrienols and β-carotene in crown daisy, unripe hot pepper, onion, garlic, and red pepper as affected by various domestic cooking methods, those were, boiling, baking, stir-frying, deep-frying, steaming, roasting, and microwaving. Fatty acid compositions were determined by GC, and HPLC were used for quantification of α-tocopherol, tocotrienols, and β-carotene. True retentions of α-tocopherol in cooked foods were as follows: boiling (77...
2017: PloS One
https://www.readbyqxmd.com/read/28452009/detection-of-potential-infectious-enteric-viruses-in-fresh-produce-by-rt-qpcr-preceded-by-nuclease-treatment
#17
Elisabet Marti, Monique Ferrary-Américo, Célia Regina Monte Barardi
Foodborne illnesses associated with contaminated fresh produce are a common public health problem and there is an upward trend of outbreaks caused by enteric viruses, especially human noroviruses (HNoVs) and hepatitis A virus (HAV). This study aimed to assess the use of DNase and RNase coupled to qPCR and RT-qPCR, respectively, to detect intact particles of human adenoviruses (HAdVs), HNoV GI and GII and HAV in fresh produce. Different concentrations of DNase and RNase were tested to optimize the degradation of free DNA and RNA from inactivated HAdV and murine norovirus (MNV), respectively...
April 27, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28450021/volatile-composition-and-sensory-characteristics-of-onion-powders-prepared-by-convective-drying
#18
So Mang Choi, Dong-Jin Lee, Jong-Yea Kim, Seung-Taik Lim
Volatile composition and sensory characteristics of onion powders prepared by convective drying at different temperatures (50, 70, and 90°C) were investigated. Dipropyl disulfide was the major volatile compound in fresh onion (77.70% of total volatile compounds). However it was considerably lost during drying, reaching 6.93-32.25µg/g solids. Dipropyl disulfide showed a positive correlation with green sensory attribute perceived by descriptive sensory analysis. Thiophenes, which were responsible for caramel and sweet attributes, were produced by drying especially when the drying temperature was high...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28447706/insights-into-the-surface-chemistry-and-electronic-properties-of-sp-2-and-sp-3-hybridized-nanocarbon-materials-for-catalysis
#19
Yangming Lin, Zhenbao Feng, Linhui Yu, Qinging Gu, Shuchang Wu, Dang Sheng Su
Ultra-dispersed nanodiamond and its derivatives (UNDDs), including bucky nanodiamond and onion-like carbon, offer superior catalytic behavior relative to other nanocarbons. However, a systematic study of their unique properties has been rarely achieved. Their surface chemistry and electronic properties are therefore studied to reveal the essential differences of UNDDs compared to other nanocarbons for catalysis.
April 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28440966/lead-levels-in-vegetables-from-artisanal-mining-sites-of-dilimi-river-bukuru-and-barkin-ladi-north-central-nigeria-cancer-and-non-cancer-risk-assessment
#20
Orish Ebere Orisakwe, Emmanuel Ayuba Dagur, Herbert Orji Chidi Mbagwu, Nnaemeka Arinze Udowelle
Lead (Pb) contamination of foods and especially of frequently consumed vegetables is a growing public health concern worldwide. Although levels of exposure in developed countries have declined over the past decades, the same cannot be said of developing countries. Health risk assessment has increasingly been employed to determine the potential hazard of heavy metal exposure to humans. In this study vegetable samples (tomatoes, red pepper, brown beans, lettuce, cabbage, Irish potatoes, onions, green beans and carrot), soil samples, irrigation water and sediment samples were collected from the Dilimi River, Bukuru and Barkin Ladi communities in north central Nigeria and analyzed for Pb content using atomic absorption spectroscopy...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
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