Alison C Ludzki, Mattias Hansen, Danae Zareifi, Jutta Jalkanen, Zhiqiang Huang, Muhmmad Omar-Hmeadi, Gianluca Renzi, Felix Klingelhuber, Sebastian Boland, Yohannes A Ambaw, Na Wang, Anastasios Damdimopoulos, Jianping Liu, Tomas Jernberg, Paul Petrus, Peter Arner, Natalie Krahmer, Rongrong Fan, Eckardt Treuter, Hui Gao, Mikael Rydén, Niklas Mejhert
Defects in adipocyte lipolysis drive multiple aspects of cardiometabolic disease, but the transcriptional framework controlling this process has not been established. To address this, we performed a targeted perturbation screen in primary human adipocytes. Our analyses identified 37 transcriptional regulators of lipid mobilization, which we classified as (i) transcription factors, (ii) histone chaperones, and (iii) mRNA processing proteins. On the basis of its strong relationship with multiple readouts of lipolysis in patient samples, we performed mechanistic studies on one hit, ZNF189 , which encodes the zinc finger protein 189...
January 5, 2024: Science Advances