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Aptamers

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https://www.readbyqxmd.com/read/28816436/integrated-distance-based-origami-paper-analytical-device-id-opad-for-one-step-visualized-analysis
#1
Tian Tian, Yuan An, Yiping Wu, Yanling Song, Zhi Zhu, Chaoyong James Yang
An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user-friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification and visual signal output, based on aptamer/invertase functionalized sepharose beads, cascaded enzymatic reactions and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, while sepharose beads with larger size are excluded and stay in the upper zone...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28811024/aptamer-induced-multicoloured-au-ncs-mos2-switch-on-fluorescence-resonance-energy-transfer-biosensor-for-dual-color-simultaneous-detection-of-multiple-tumor-markers-by-single-wavelength-excitation
#2
Shenghao Xu, Xiuying Feng, Teng Gao, Gufan Liu, Yaning Mao, Jiehua Lin, Xijuan Yu, Xiliang Luo
An aptamer induced "switch on" fluorescence resonance energy transfer (FRET) biosensor for the simultaneous detection of multiple tumor markers (e.g., AFP and CEA) combining molybdenum disulfide (MoS2) nanosheets with multicolored Au NCs by a single excitation was developed for the first time. Here, AFP aptamer functionalized green colored Au NCs (510 nm) and CEA aptamer functionalized red colored Au NCs (650 nm) are used as energy donors, while MoS2 is used as energy receptor. On the basis of recording the change of the recovered fluorescence intensity at 510 nm and 650 nm upon the addition of targets CEA and AFP, these two tumor markers can be simultaneously quantitatively detected, with detection limits of 0...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28810234/electrochemical-aptamer-based-nanosensor-fabricated-on-single-au-nanowire-electrodes-for-adenosine-triphosphate-assay
#3
Dongmei Wang, Xiaoqing Xiao, Shen Xu, Yong Liu, Yongxin Li
In this work, single Au nanowire electrodes (AuNWEs) were fabricated by laser-assisted pulling/hydrofluoric acid (HF) etching process, which then were characterized by transmission electron microscopy (TEM), electrochemical method and finite-element simulation. The as-prepared single AuNWEs were used to construct electrochemical aptamer-based nanosensors (E-AB nanosensors) based on the formation of Au-S bond that duplex DNA tagged with methylene blue (MB) was modified on the surface of electrode. In the presence of adenosine triphosphate (ATP), the MB-labeled aptamer dissociated from the duplex DNA due to the strong specific affinity between aptamer and target, which lead to the reduction of MB electrochemical signals...
August 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28809082/development-of-a-dual-functional-hydrogel-using-rgd-and-anti-vegf-aptamer
#4
Nan Zhao, Mark R Battig, Ming Xu, Xiuli Wang, Na Xiong, Yong Wang
Synthetic molecular libraries hold great potential to advance the biomaterial development. However, little effort is made to integrate molecules with molecular recognition abilities selected from different libraries into a single biomolecular material. The purpose of this work is to incorporate peptides and nucleic acid aptamers into a porous hydrogel to develop a dual-functional biomaterial. The data show that an anti-integrin peptide can promote the attachment and growth of endothelial cells in a 3D porous poly(ethylene glycol) hydrogel and an antivascular endothelial growth factor aptamer can sequester and release VEGF of high bioactivity...
August 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28806700/a-large-raman-scattering-cross-section-molecular-embedded-sers-aptasensor-for-ultrasensitive-aflatoxin-b1-detection-using-cs-fe3o4-for-signal-enrichment
#5
Quansheng Chen, Mingxiu Yang, Xiaojing Yang, Huanhuan Li, Zhiming Guo, M H Rahma
With growing concern on oil safety problems, developing a simple and sensitive method to detect Aflatoxin B1 (AFB1), a common mycotoxin in peanut oil, is very necessary. In this study, Surface-enhanced Raman Scattering (SERS) aptasensor was developed for ultrasensitive AFB1 detection using the amino-terminal AFB1 aptamer (NH2-DNA1); and thiol-terminal AFB1 complementary aptamer (SH-DNA2) conjugated magnetic-beads (CS-Fe3O4) as enrichment nanoprobe and AuNR@DNTB@Ag nanorods (ADANRs) as reporter nanoprobe respectively...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28806695/use-of-peptide-aptamers-cationic-peptides-and-artificial-zinc-finger-proteins-to-generate-resistance-to-plant-viruses
#6
REVIEW
Takashi Sera
Various RNA/DNA viruses have caused severe infectious diseases in plants as well as animals, including humans, and been a threat to the production of agricultural crops. Therefore, prevention of plant virus infections is a major objective in crop protection. One attractive approach is to inhibit functions of viral proteins responsible for virus infections. In this review, I describe the status using such approaches to confer virus resistance to plants by three types of peptides/proteins: peptide aptamers, artificial zinc finger proteins and acidic peptides...
August 11, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28806557/a-highly-sensitive-and-selective-aptamer-based-colorimetric-sensor-for-the-rapid-detection-of-pcb-77
#7
Ruojie Cheng, Siyao Liu, Huijie Shi, Guohua Zhao
A highly sensitive, specific and simple colorimetric sensor based on aptamer was established for the detection of polychlorinated biphenyls (PCB 77). The use of unmodified gold nanoparticles as a colorimetric probe for aptamer sensors enabled the highly sensitive and selective detection of polychlorinated biphenyls (PCB 77). A linear range of 0.5nM to 900nM was obtained for the colorimetric assay with a minimum detection limit of 0.05nM. In addition, by the methods of circular dichroism, UV and naked eyes, we found that the 35 base fragments retained after cutting 5 bases from the 5 'end of aptamer plays the most significant role in the PCB 77 specific recognition process...
July 25, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28805744/aptamer-cell-based-selection-overview-and-advances
#8
REVIEW
Silvia Catuogno, Carla Lucia Esposito
Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools. Among selection strategies, procedures using living cells as complex targets (referred as "cell-SELEX") have been developed as an effective mean to generate aptamers for heavily modified cell surface proteins, assuring the binding of the target in its native conformation. Here we give an up-to-date overview on cell-SELEX technology, discussing the most recent advances with a particular focus on cancer cell targeting...
August 14, 2017: Biomedicines
https://www.readbyqxmd.com/read/28804757/one-aptamer-two-functions-the-full-length-aptamer-inhibits-ampa-receptors-while-the-short-one-inhibits-both-ampa-and-kainate-receptors
#9
William J Jaremko, Zhen Huang, Wei Wen, Andrew Wu, Nicholas Karl, Li Niu
AMPA and kainate receptors, along with NMDA receptors, are distinct subtypes of glutamate ion channels. Excessive activity of AMPA and kainate receptors has been implicated in neurological diseases, such as epilepsy and neuropathic pain. Antagonists that block their activities are therefore potential drug candidates. In a recent article in the Journal of Biological Chemistry by Jaremko et al. 2017, we have reported on the discovery and molecular characterization of an RNA aptamer of a dual functionality: the full-length RNA (101 nucleotide) inhibits AMPA receptors while the truncated or the short (55 nucleotide) RNA inhibits both the AMPA and kainate receptors...
2017: RNA & Disease
https://www.readbyqxmd.com/read/28803753/recent-progresses-in-biomedical-applications-of-aptamer-functionalized-systems
#10
REVIEW
Fei Ding, Yangguang Gao, Xianran He
Aptamers, known as "chemical antibodies" are screened via a combinational technology of systematic evolution of ligands by exponential enrichment (SELEX). Due to their specific targeting ability, high binding affinity, low immunogenicity and easy modification, aptamer-functionalized systems have been extensively applied in various fields and exhibit favorable results. However, there is still a long way for them to be commercialized, and few aptamer-functionalized systems have yet successfully entered clinical and industrial use...
March 18, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28803475/intuitive-label-free-sers-detection-of-bacteria-using-aptamer-based-in-situ-ag-nanoparticles-synthesis
#11
Weicun Gao, Bo Li, Ruizhi Yao, Zhiping Li, Xiwen Wang, Xiaolin Dong, Han Qu, Qianxue Li, Nan Li, Hang Chi, Bo Zhou, Zhiping Xia
The characteristic of an ideal bacteria-detection method should have high sensitivity, specificity, easy to operate and without time-consuming culture process. In this study, we report a new bacteria detection strategy that can recognize bacteria quickly and directly by surface-enhanced Raman scattering (SERS) with the formation of well-defined Bacteria-aptamer@AgNPs. SERS signals generated by Bacteria-aptamer@AgNPs exhibited a linear dependence on bacteria (R2=0.9671) concentration ranged from 101 to 107 cfu/mL...
August 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28800954/a-tim-3-oligonucleotide-aptamer-enhances-t-cell-functions-and-potentiates-tumor-immunity-in-mice
#12
Tal Gefen, Iris Castro, Darija Muharemagic, Yvonne Puplampu-Dove, Shradha Patel, Eli Gilboa
T cell immunoglobulin-3 (TIM-3) is a negative regulator of interferon-γ (IFN-γ) secreting CD4(+) T cells and CD8(+) T cytotoxic cells. Recent studies have highlighted the role of TIM-3 as an important mediator of CD8(+) T cell exhaustion in the setting of chronic viral infections and cancer. In murine tumor models, antibody blockade of TIM-3 with anti-TIM-3 antibodies as monotherapy has no or minimal antitumor activity, suggesting that TIM-3 signaling exerts an accessory or amplifying effect in keeping immune responses in check...
August 8, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28800505/electrochemical-aptasensor-for-thrombin-using-co-catalysis-of-hemin-g-quadruplex-dnazyme-and-octahedral-cu2o-au-nanocomposites-for-signal-amplification
#13
Shuai Chen, Pin Liu, Kewen Su, Xuan Li, Zhen Qin, Wei Xu, Jun Chen, Chaorui Li, Jingfu Qiu
In this work, novel octahedral Cu2O-Au nanocomposites were synthesized and first applied in an electrochemical aptasensor to detect thrombin (TB) with the aid of a DNAzyme for signal amplification. The octahedral Cu2O-Au nanocomposites have not only simultaneously served as signal amplifying molecules but have also been utilized as an ideal loading platform to immobilize a large number of electroactive substances and recognition probes. Gold nanoparticles (AuNPs) were grown directly on the surface of the octahedral Cu2O nanocrystals, and the Cu2O-Au nanocomposites obtained had the advantages of large surface areas and excellent biocompatibilities...
August 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28799602/ultra-sensitive-dna-sensing-of-a-prostate-specific-antigen-based-on-2d-nanosheets-in-live-cells
#14
Namasivayam Dhenadhayalan, Kanchan Yadav, Muthu Irulappan Sriram, Hsin-Lung Lee, King-Chuen Lin
Herein, we report ultra-sensitive sensing of a prostate-specific antigen (PSA), which is used as a biomarker to detect prostate cancer, using a molybdenum series (MoO3, MoS2, and MoSe2) of two-dimensional nanosheets (2D NSs). Moreover, the design of a 2D NS-based PSA aptamer sensor system was demonstrated based on a fluorescence turn-on mechanism in the presence of a target. The 2D NSs acted as an excellent sensing platform in which the PSA aptamer was adsorbed on the NSs and subsequent energy transfer between them led to fluorescence quenching of the aptamer...
August 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28796475/detection-of-plasmodium-lactate-dehydrogenase-pldh-antigen-in-buffer-using-aptamer-modified-magnetic-microparticles-for-capture-oligonucleotide-modified-quantum-dots-for-detection-and-oligonucleotide-modified-gold-nanoparticles-for-signal-amplification
#15
Chloe Kim, Peter C Searson
To overcome the limitations associated with antibody-based sensors, we describe a proof-of-concept of an aptamer-based sandwich assay for detection of lactate dehydrogenase, an antigen associated with malaria. We show a detection limit of Plasmodium falciparum lactate dehydrogenase and Plasmodium vivax lactate dehydrogenase of 0.5 fmole in buffer, comparable to an antibody-based assay, using a magnetic particle - aptamer construct for capture, and a quantum dot - aptamer construct for detection. We then demonstrate a detection limit of 10 amole (50-fold amplification) using oligonucleotide-functionalized gold nanoparticles to allow conjugation of multiple quantum dots for each target antigen...
August 10, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28795534/graphene-field-effect-transistors-for-the-sensitive-and-selective-detection-of-escherichia-coli-using-pyrene-tagged-dna-aptamer
#16
Guangfu Wu, Ziwen Dai, Xin Tang, Zihong Lin, Pik Kwan Lo, Meyya Meyyappan, King Wai Chiu Lai
This study reports biosensing using graphene field-effect transistors with the aid of pyrene-tagged DNA aptamers, which exhibit excellent selectivity, affinity, and stability for Escherichia coli (E. coli) detection. The aptamer is employed as the sensing probe due to its advantages such as high stability and high affinity toward small molecules and even whole cells. The change of the carrier density in the probe-modified graphene due to the attachment of E. coli is discussed theoretically for the first time and also verified experimentally...
August 10, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28795444/prediction-of-shorter-oligonucleotide-sequences-recognizing-aflatoxin-m1
#17
Amit Kumar Pandey, Yudhishthir Singh Rajput, Dheer Singh, Rajan Sharma
Aflatoxin M1 is present in milk of lactating animals fed on aflatoxin B1 contaminated feeds. Aptamers are new emerging ligand molecules and can be employed in assay protocols. Shorter aptamers have several advantages. Untruncated (72 nt long) and truncated (18 to 42 nts long) aptamers were evaluated for aflatoxin M1 recognition which was detected by colour-change in aptamer-conjugated-gold nanoparticles in presence of aflatoxin M1. Truncation of 10 aptamers was designed to retain motifs. Untruncated and truncated aptamers recognised aflatoxin M1...
August 10, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28794815/detection-of-c-reactive-protein-on-an-integrated-microfluidic-system-by-utilizing-field-effect-transistors-and-aptamers
#18
Wei-Chieh Kao, Yen-Wen Chen, Chia-Ho Chu, Wen-Hsin Chang, Shu-Chu Shiesh, Yu-Lin Wang, Gwo-Bin Lee
Cardiovascular diseases (CVDs) cause more than 17 × 10(6) deaths worldwide on a yearly basis. Early diagnosis of CVDs is therefore of great need. The C-reactive protein (CRP) is an important biomarker for analyzing the risks of CVDs. In this work, CRP-specific aptamers with high sensitivity and specificity and field-effect-transistor (FET) devices were used to recognize and detect CRP by using an integrated microfluidic system automatically while consuming less volumes of reagents and samples (about 5 μm)...
July 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28794625/paclitaxel-loaded-and-a10-3-2-aptamer-targeted-poly-lactide-co-glycolic-acid-nanobubbles-for-ultrasound-imaging-and-therapy-of-prostate-cancer
#19
Meng Wu, Ying Wang, Yiru Wang, Mingbo Zhang, Yukun Luo, Jie Tang, Zhigang Wang, Dong Wang, Lan Hao, Zhibiao Wang
In the current study, we synthesized prostate cancer-targeting poly(lactide-co-glycolic acid) (PLGA) nanobubbles (NBs) modified using A10-3.2 aptamers targeted to prostate-specific membrane antigen (PSMA) and encapsulated paclitaxel (PTX). We also investigated their impact on ultrasound (US) imaging and therapy of prostate cancer. PTX-A10-3.2-PLGA NBs were developed using water-in-oil-in-water (water/oil/water) double emulsion and carbodiimide chemistry approaches. Fluorescence imaging together with flow cytometry verified that the PTX-A10-3...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28794178/potential-of-high-affinity-slow-off-rate-modified-aptamer-somamer-reagents-for-mycobacterium-tuberculosis-proteins-as-tools-for-infection-models-and-diagnostic-applications
#20
Theresa Russell, Louis S Green, Taylor Rice, Nicole A Kruh-Garcia, Karen Dobos, Mary A DeGroote, Thomas Hraha, David G Sterling, Nebojsa Janjic, Urs A Ochsner
Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult, due to low levels of circulating antigens or due to the lack of specific, high-affinity binding reagents and reliable assays with adequate sensitivity. We sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with sub-nanomolar affinity for M. tuberculosis (Mtb) proteins (antigen 85A, 85B, 85C, GroES, GroEL2, DnaK, CFP10, KAD, CFP2, RplL and Tpx) could be useful to diagnose tuberculosis. When incorporated into the multiplexed, array-based proteomic SOMAscan assay, limits of detection reached the sub-picomolar range in 40% serum...
August 9, 2017: Journal of Clinical Microbiology
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