keyword
MENU ▼
Read by QxMD icon Read
search

Aptamers

keyword
https://www.readbyqxmd.com/read/29332836/fluorescent-and-colorimetric-dual-mode-aptasensor-for-thrombin-detection-based-on-target-induced-conjunction-of-split-aptamer-fragments
#1
Wenna Duan, Xiuzhong Wang, Haixia Wang, Feng Li
Since the lack of detection diversity of the single-signal readout strategy, it is urgent to develop fast and multisignal assay strategies. A highly selective and sensitive assay method with colorimetric and fluorometric dual signals readouts is presented in this paper. It is based on the principle that the target induced conjunction of split aptamer fragments assembled on the surface of Au nanoparticles (AuNPs). In the presence of targets, the color of solution changed from wine red to blue and can be measured both visual inspection and spectrophotometry because of the aggregation of AuNPs...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331900/a-catalytic-and-dual-recycling-amplification-atp-sensor-based-on-target-driven-allosteric-structure-switching-of-aptamer-beacons
#2
Ying Peng, Daxiu Li, Ruo Yuan, Yun Xiang
Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29329202/epcam-immunotherapy-versus-specific-targeted-delivery-of-drugs
#3
REVIEW
Joanna Macdonald, Justin Henri, Kislay Roy, Emma Hays, Michelle Bauer, Rakesh Naduvile Veedu, Normand Pouliot, Sarah Shigdar
The epithelial cell adhesion molecule (EpCAM), or CD326, was one of the first cancer associated biomarkers to be discovered. In the last forty years, this biomarker has been investigated for use in personalized cancer therapy, with the first monoclonal antibody, edrecolomab, being trialled in humans more than thirty years ago. Since then, several other monoclonal antibodies have been raised to EpCAM and tested in clinical trials. However, while monoclonal antibody therapy has been investigated against EpCAM for almost 40 years as primary or adjuvant therapy, it has not shown as much promise as initially heralded...
January 12, 2018: Cancers
https://www.readbyqxmd.com/read/29327939/blockade-of-the-complement-c5a-c5ar1-axis-impairs-lung-cancer-bone-metastasis-by-cxcl16-mediated-effects
#4
Daniel Ajona, Carolina Zandueta, Leticia Corrales, Haritz Moreno, María J Pajares, Sergio Ortiz-Espinosa, Elena Martínez-Terroba, Naiara Perurena, Fernando J de Miguel, Eloisa Jantus-Lewintre, Carlos Camps, Silvestre Vicent, Jackeline Agorreta, Luis M Montuenga, Ruben Pio, Fernando Lecanda
RATIONALE: C5aR1(CD88), a receptor for complement anaphylatoxin C5a, is a potent immune mediator. Its impact on malignant growth and dissemination of NSCLC cells is poorly understood. OBJECTIVES: To investigate the contribution of the C5a/C5aR1 axis to the malignant phenotype of NSCLC cells, particularly in skeletal colonization, a preferential lung metastasis site. METHODS: Association between C5aR1 expression and clinical outcome was assessed in silico and validated by immunohistochemistry...
January 12, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29327496/modulation-of-bace1-activity-by-chemically-modified-aptamers
#5
Cécile Gasse, Marwa Zaarour, Sam Noppen, Mikhail Abramov, Philippe Marlière, Sandra Liekens, Bart De Strooper, Piet Herdewijn
A modified DNA aptamer was developed that binds BACE1, a therapeutic target involved in Alzheimer's disease. This ssXNA not only tightly binds to BACE1 but also inhibits its protease activity in vitro in the same range as a previously described unmodified aptamer. We report the in vitro selection of functional oligonucleotides using two nucleobase modifications: 5-chlorouracil and 7-deazaadenine. The nucleoside analog 5-chloro-2'-deoxyuridine has already been explored to replace thymidine in a chemically modified genome of a bacterium...
January 11, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29325460/targeted-delivery-of-melittin-to-cancer-cells-by-as1411-anti-nucleolin-aptamer
#6
Seyed Hossein Rajabnejad, Ahad Mokhtarzadeh, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Bibi Marjan Razavi
Melittin, a small water-soluble cationic amphipathic α-helical linear peptide, consisted of 26 amino acids, is the honeybee venom major constituent. Several reports have proved the lytic and apoptotic effects of melittin in several cancerous cell lines. In this study, we aimed to fabricate an AS1411 aptamer-melittin to specifically deliver melittin to nucleolin positive cells (A549). Melittin was covalently attached to antinucleolin aptamer (AS1411) and its toxicity in A549 (Nucleolin positive) and L929 (Nucleolin negative) was studied using MTT and Annexin V flow cytometry methods...
January 12, 2018: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/29325019/deep-molecular-phenotypes-link-complex-disorders-and-physiological-insult-to-cpg-methylation
#7
Shaza B Zaghlool, Dennis O Mook-Kanamori, Sara Kader, Nisha Stephan, Anna Halama, Rudolf Engelke, Hina Sarwath, Eman K Al-Dous, Yasmin A Mohamoud, Werner Roemisch-Margl, Jerzy Adamski, Gabi Kastenmüller, Nele Friedrich, Alessia Visconti, Pei-Chien Tsai, Tim Spector, Jordana Bell, Mario Falchi, Annika Wahl, Melanie Waldenberger, Annette Peters, Christian Gieger, Maija Pezer, Gordan Lauc, Johannes Graumann, Joel A Malek, Karsten Suhre
Epigenetic regulation of cellular function provides a mechanism for rapid organismal adaptation to changes in health, lifestyle, and environment. Associations of cytosine-guanine di-nucleotide (CpG) methylation with clinical endpoints that overlap with metabolic phenotypes suggest a regulatory role for these CpG sites in the body's response to disease or environmental stress. We previously identified 20 CpG sites in an epigenome-wide association study (EWAS) with metabolomics that were also associated in recent EWASs with diabetes-, obesity-, and smoking-related endpoints...
January 8, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29323871/microfluidic-device-for-aptamer-based-cancer-cell-capture-and-genetic-mutation-detection
#8
Sarah Jessica Reinholt, Harold G Craighead
We present a microfluidic device for specifically capturing cancer cells and isolating their genomic DNA (gDNA) for specific amplification and sequence analysis. To capture cancer cells within the device, nucleic acid aptamers that specifically bind to cancer cells were immobilized within a channel containing micropillars designed to increase capture efficiency. The cap-tured cells were lysed in situ and their gDNA was isolated by physical entanglement within a second smaller-dimensioned micropillar array. This type of isolation allowed the gDNA to be retained and purified within the channel, and enables ampli-fication and analysis to be performed on the gDNA without the loss of the original template...
January 11, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29322773/sers-based-label-free-insulin-detection-at-physiological-concentrations-for-analysis-of-islet-performance
#9
Hyunjun Cho, Shailabh Kumar, Daejong Yang, Sagar R Vaidyanathan, Kelly Woo, Ian Garcia, Hao Jan Shue, Youngzoon Yoon, Kevin Ferreri, Hyuck Choo
Label-free optical detection of insulin would allow in vitro assessment of pancreatic cell functions in their natural state and expedite diabetes-related clinical research and treatment, however no existing method has met these criteria at physiological concentrations. Using spatially-uniform 3D gold-nanoparticle sensors, we have demonstrated surface-enhanced Raman sensing of insulin in the secretions from human pancreatic islets under low and high glucose environments without the use of labels such as antibodies or aptamers...
January 11, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29317345/muc1-aptamer-targeted-dna-micelles-for-dual-tumor-therapy-using-doxorubicin-and-kla-peptide
#10
Fahimeh Charbgoo, Mona Alibolandi, Seyed Mohammad Taghdisi, Khalil Abnous, Fatemeh Soltani, Mohammad Ramezani
Targeted delivery of DNA nanoparticles is a promising approach in cancer therapy. Using aptamers, target specific delivery of DNA nanoparticles can be achieved. Further, aptamers can indirectly improve drug encapsulation efficiency of DNA nanoparticles for drugs intercalated within nucleic acid base pairs. Using DNA blocks, a micellar hybrid nanoparticle was prepared for the targeted co-delivery of doxorubicin and a pro-apoptotic peptide, KLA to tumor cells. Results demonstrated that anti-MUC1 aptamer could specifically deliver the synthesized DNA micelle into MCF-7 cells by improving its cellular uptake...
January 6, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29315675/chemical-modifications-of-nucleic-acid-drugs-and-their-delivery-systems-for-gene-based-therapy
#11
REVIEW
Changmai Chen, Zhenjun Yang, Xinjing Tang
Gene-based therapy is one of essential therapeutic strategies for precision medicine through targeting specific genes in specific cells of target tissues. However, there still exist many problems that need to be solved, such as safety, stability, selectivity, delivery, as well as immunity. Currently, the key challenges of gene-based therapy for clinical potential applications are the safe and effective nucleic acid drugs as well as their safe and efficient gene delivery systems. In this review, we first focus on current nucleic acid drugs and their formulation in clinical trials and on the market, including antisense oligonucleotide, siRNA, aptamer, and plasmid nucleic acid drugs...
January 5, 2018: Medicinal Research Reviews
https://www.readbyqxmd.com/read/29313532/a-label-free-versatile-and-low-background-chemiluminescence-aptasensing-strategy-based-on-gold-nanocluster-catalysis-combined-with-the-separation-of-magnetic-beads
#12
Yueyue Yao, Xiuzhong Wang, Wenna Duan, Feng Li
A label-free, versatile and low-background chemiluminescence (CL) sensing strategy based on gold nanocluster catalysis combined with the separation of magnetic beads (MBs) was developed. Kanamycin was selected as the target analyte to exhibit the analytical performance of this platform. Two single-stranded DNA (named DNA1 and DNA2) are ingeniously designed. DNA1, containing an aptamer sequence of the targets, was firstly immobilized on the MBs which were modified with many amino groups by amidation reaction...
January 9, 2018: Analyst
https://www.readbyqxmd.com/read/29313393/ultrasound-mediated-nanobubble-destruction-umnd-facilitates-the-delivery-of-a10-3-2-aptamer-targeted-and-sirna-loaded-cationic-nanobubbles-for-therapy-of-prostate-cancer
#13
Meng Wu, Hongyun Zhao, Liang Guo, Yiru Wang, Jiao Song, Xueli Zhao, Chongyan Li, Lan Hao, Dong Wang, Jie Tang
The Forkhead box M1 (FoxM1) transcription factor is an important anti-tumor target. A novel targeted ultrasound (US)-sensitive nanobubble that is likely to make use of the physical energy of US exposure for the improvement of delivery efficacy to target tumors and specifically silence FoxM1 expression appears as among the most potential nanocarriers in respect of drug delivery. In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29313333/dithiothreitol-regulated-coverage-of-oligonucleotide-modified-gold-nanoparticles-to-achieve-optimized-biosensor-performance
#14
Pingping Liang, Juan Canoura, Haixiang Yu, Obtin Alkhamis, Yi Xiao
DNA-modified gold nanoparticles (AuNPs) are useful signal-reporters for the detection of a variety of molecules via different hybridization- and enzyme-based assays. However, their performance is heavily dependent on the surface coverage of the immobilized probe DNA, which can influence both target binding and enzymatic processing of the bound probes. Current methods used to adjust DNA surface coverage on AuNPs require the production of multiple batches of AuNPs under different conditions, which is costly and laborious...
January 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29311281/cyclic-di-gmp-regulates-tfoy-in-vibrio-cholerae-to-control-motility-by-both-transcriptional-and-posttranscriptional-mechanisms
#15
Benjamin R Pursley, Michael M Maiden, Meng-Lun Hsieh, Nicolas Fernandez, Geoffrey B Severin, Christopher M Waters
Cyclic di-GMP (c-di-GMP) is a bacterial second messenger molecule that is a key global regulator in Vibrio cholerae, but the molecular mechanisms by which this molecule regulates downstream phenotypes have not been fully characterized. One such regulatory factor that may respond to c-di-GMP is the Vc2 c-di-GMP-binding riboswitch that is hypothesized to control the expression of the downstream putative transcription factor TfoY. Although much is known about the physical and structural properties of the Vc2 riboswitch aptamer, the nature of its expression and function in V...
January 8, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29310319/a-paper-based-sers-test-strip-for-quantitative-detection-of-mucin-1-in-whole-blood
#16
Shan-Wen Hu, Shu Qiao, Jian-Bin Pan, Bin Kang, Jing-Juan Xu, Hong-Yuan Chen
A paper-based SERS test strip combining strengths of paper chip and Raman active substrate was demonstrated to overcome challenges in spectroscopic sensing of complicated samples and realize quantitative detection of disease markers in whole blood. The precisely controlled Au NPs were not only capable of generating condensed hot spots on the fibers, but also enhanced the size exclusion effect of paper, resulting in the novel performance on both SERS detection and sample pretreatment. A biosensor for Mucin-1 is developed by equipping the Au NPs with aptamer...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310266/ultrasensitive-and-high-specific-detection-of-non-small-cell-lung-cancer-cells-in-human-serum-and-clinical-pleural-effusion-by-aptamer-based-fluorescence-spectroscopy
#17
Yuhang Wu, Hong Zhang, Junfeng Xiang, Zhihua Mao, Gang Shen, Fengmin Yang, Yan Liu, Wei Wang, Ning Du, Jinghua Zhang, Yalin Tang
Lung cancer is the leading cause of cancer-related deaths worldwide, and approximately 85% are diagnosed as non-small-cell lung cancer (NSCLC). However, efficient detection and diagnosis of NSCLC at early stage is still challenging. In this work, we developed a simple, ultrasensitive and high selective strategy for A549 human NSCLC cells detection based on combining the reorganization property of a novel cyanine dye 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (cy-M) to aptamer S6 G-quadruplex structure having specific affinity to NSCLC cells, which induced a dramatic fluorescence enhancement (~ 104 times)...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310253/an-electrochemical-aptasensor-for-the-highly-sensitive-detection-of-8-hydroxy-2-deoxyguanosine-based-on-the-hybridization-chain-reaction
#18
Li-Ping Jia, Li-Juan Wang, Rong-Na Ma, Lei Shang, Wei Zhang, Qing-Wang Xue, Huai-Sheng Wang
In the present work a highly sensitive and selective aptasensor was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) based on the hybridization chain reaction (HCR) signal amplification. It was observed that the aptamer of 8-OH-dG could hybridize with the capture DNA immobilized on the gold electrode with a sticky tail left, which initiated the HCR and led to the formation of extended dsDNA structure on the electrode surface. Then the electroactive species ([Ru(NH3)6]3+, RuHex) intercalated into the dsDNA grooves to generate the amplified signal...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310244/a-dual-targeting-dna-tetrahedron-nanocarrier-for-breast-cancer-cell-imaging-and-drug-delivery
#19
Xiaoting Liu, Lina Wu, Lei Wang, Wei Jiang
To enhance efficacy of chemotherapy and achieve real-time imaging of cancer cells, it is crucial to develop nanocarriers with targeted drug delivery capacity and fluorescence property for cancer theranostics. Herein, a dual-targeting DNA tetrahedron nanocarrier (MUC1-Td-AS1411) was constructed for breast cancer cell imaging and targeted drug delivery. This nanocarrier consisted of three components: (i) DNA tetrahedron core for multivalent conjugation of function ligands and loading doxorubicin (Dox); (ii) activatable MUC1 aptamer probe (MUC1-probe), formed by the hybridization of MUC1 aptamer sequence with fluorophore extended from one vertex and complementary sequence with quencher, for targeting and imaging MUC1 protein on cytomembrane; (iii) AS1411 aptamer, which was hybridized to the overhang on three vertexes via prolonged sequence, for binding to nucleolin...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310242/competitive-horseradish-peroxidase-linked-aptamer-assay-for-sensitive-detection-of-aflatoxin-b1
#20
Linlin Sun, Qiang Zhao
Aflatoxin B1 (AFB1) is one of highly toxic mycotoxins and a known human carcinogen. The frequent contamination of AFB1 in food products and large health risk of AFB1 have raised global concerns. Sensitive detection of AFB1 is of vital importance and highly demanded. Herein, we reported a competitive horseradish peroxidase (HRP)-linked aptamer assay for AFB1, combining the advantages of aptamer for affinity binding and enzyme label for signal amplification. In this assay, free AFB1 in solution competed with a covalent conjugate of bovine serum albumin-AFB1 (BSA-AFB1) coated on the wells of microplate in binding to the HRP-labeled aptamer probe...
March 1, 2018: Talanta
keyword
keyword
41159
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"