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Hemorrhagic Fever viruses

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https://www.readbyqxmd.com/read/29340221/recombinant-antibodies-to-the-ebola-virus-glycoprotein
#1
A A Panina, I G Dementieva, T K Aliev, V A Toporova, D S Balabashin, M N Bokov, L P Pozdnyakova, O B Shchemchukova, D A Dolgikh, P G Sveshnikov, M P Kirpichnikov
Currently, there are no approved therapies for targeted prevention and treatment of Ebola hemorrhagic fever. In the present work, we describe the development of a eukaryotic expression system for the production of three full-length chimeric antibodies (IgG1-kappa isotypes) GPE118, GPE325, and GPE534 to the recombinant glycoprotein of the Ebola virus (EBOV GP), which is a key factor in the pathogenicity of the disease. The immunochemical properties of the obtained antibodies were studied by immunoblotting and indirect, direct, and competitive ELISA using the recombinant EBOV proteins rGPdTM, NP, and VP40...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29338543/lucidone-suppresses-dengue-viral-replication-through-the-induction-of-heme-oxygenase-1
#2
Wei-Chun Chen, Chin-Kai Tseng, Chun-Kuang Lin, Shen-Nien Wang, Wen-Hung Wang, Shih-Hsien Hsu, Yu-Hsuan Wu, Ling-Chien Hung, Yen-Hsu Chen, Jin-Ching Lee
Dengue virus (DENV) infection causes life-threatening diseases such as dengue hemorrhagic fever and dengue shock syndrome. Currently, there is no effective therapeutic agent or vaccine against DENV infection; hence, there is an urgent need to discover anti-DENV agents. The potential therapeutic efficacy of lucidone was first evaluated in vivo using a DENV-infected Institute of Cancer Research (ICR) suckling mouse model by monitoring body weight, clinical score, survival rate, and viral titer. We found that lucidone effectively protected mice from DENV infection by sustaining survival rate and reducing viral titers in DENV-infected ICR suckling mice...
January 17, 2018: Virulence
https://www.readbyqxmd.com/read/29337162/antiviral-activity-of-k22-against-members-of-the-order-nidovirales
#3
Julie Christiane Françoise Rappe, Adriaan de Wilde, Han Di, Christin Müller, Hanspeter Stalder, Philip V'kovski, Eric Snijder, Margo A Brinton, John Ziebuhr, Nicolas Ruggli, Volker Thiel
Recently, a novel antiviral compound (K22) that inhibits replication of a broad range of animal and human coronaviruses was reported to interfere with viral RNA synthesis by impairing double-membrane vesicle (DMV) formation (Lundin et al., 2014). Here we assessed potential antiviral activities of K22 against a range of viruses representing two (sub)families of the order Nidovirales, the Arteriviridae (porcine reproductive and respiratory syndrome virus [PRRSV], equine arteritis virus [EAV] and simian hemorrhagic fever virus [SHFV]), and the Torovirinae (equine torovirus [EToV] and White Bream virus [WBV])...
January 11, 2018: Virus Research
https://www.readbyqxmd.com/read/29330092/a-novel-double-antigen-sandwich-elisa-for-the-species-independent-detection-of-crimean-congo-hemorrhagic-fever-virus-specific-antibodies
#4
Miriam A Sas, Loic Comtet, Fabien Donnet, Marc Mertens, Zati Vatansever, Noel Tordo, Philippe Pourquier, Martin H Groschup
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease in humans caused by the CCHF virus (CCHFV). The detection of anti-CCHFV antibodies in animals is used to reveal infection risk areas. Therefore a simple, quick and reliable multispecies assay for the detection of CCHFV-specific antibodies is needed. This work presents the development and validation of a novel CCHF double-antigen ELISA for the detection of anti-CCHFV nucleoprotein antibodies. The test requires 30 μl of serum, and results are obtained within 90 min...
January 9, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29321318/viperin-restricts-zika-virus-and-tick-borne-encephalitis-virus-replication-by-targeting-ns3-for-proteasomal-degradation
#5
Christakis Panayiotou, Richard Lindqvist, Chaitanya Kurhade, Kirstin Vonderstein, Jenny Pasto, Karin Edlund, Arunkumar S Upadhyay, Anna K Överby
Flaviviruses are arthropod-borne viruses that constitute a major global health problem, with millions of human infections annually. Their pathogenesis ranges from mild illness to severe manifestations such as hemorrhagic fever and fatal encephalitis. Type I interferons (IFNs) are induced in response to viral infection, and stimulate the expression of interferon-stimulated genes (ISGs), including that encoding viperin (virus-inhibitory protein, endoplasmic reticulum-associated, IFN-inducible), which shows antiviral activity against a broad spectrum of viruses including several flaviviruses...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29319426/dengue-hemorrhagic-fever-virus-in-saudi-arabia-a-review
#6
Jaffar A Al-Tawfiq, Ziad A Memish
Dengue fever is a global disease with a spectrum of clinical manifestation ranging from mild febrile disease to a severe disease in the form of dengue hemorrhagic fever and dengue shock syndrome. Dengue virus is one viral hemorrhagic fever that exists in the Kingdom of Saudi Arabia in addition to Alkhurma (Alkhurma) Hemorrhagic Fever, Chikungunya virus, Crimean-Congo Hemorrhagic Fever, and Rift Valley Fever. The disease is limited to the Western and South-western regions of Saudi Arabia, where Aedes aegypti exists...
January 10, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29315647/antiviral-activity-of-a771726-the-active-metabolite-of-leflunomide-against-jun%C3%A3-n-virus
#7
Claudia S Sepúlveda, Cybele C García, Elsa B Damonte
The aim of this study was to investigate the effect of A771726, the active metabolite of leflunomide, against the infection with Junín virus (JUNV), agent of Argentine hemorrhagic fever (AHF). The treatment with non-cytotoxic concentrations of A771726 of Vero and A549 cells infected with JUNV inhibited virus replication in a dose-dependent manner, as determined by virus yield reduction assay. The antiviral effectiveness of A771726 was not importantly affected by the multiplicity of infection and the virus strain...
January 5, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29312814/on-revealing-the-gene-targets-of-ebola-virus-micrornas-involved-in-the-human-skin-microbiome
#8
Pei-Chun Hsu, Bin-Hao Chiou, Chun-Ming Huang
Ebola virus, a negative-sense single-stranded RNA virus, causes severe viral hemorrhagic fever and has a high mortality rate. Histopathological and immunopathological analyses of Ebola virus have revealed that histopathological changes in skin tissue are associated with various degrees of endothelial cell swelling and necrosis. The interactions of microbes within or on a host are a crucial for the skin immune shield. The discovery of microRNAs (miRNAs) in Ebola virus implies that immune escape, endothelial cell rupture, and tissue dissolution during Ebola virus infection are a result of the effects of Ebola virus miRNAs...
2018: PeerJ
https://www.readbyqxmd.com/read/29298405/ultrastructural-features-of-alkhumra-hemorrhagic-fever-virus-infection-of-cells-under-in-vivo-and-in-vitro-conditions
#9
Tariq A Madani, El-Tayb M E Abuelzein, Huda Abu-Araki, Soad S Ali, Sawsan M Jalalah, Ahmed M Hassan, Hussein M S Al-Bar, Esam I Azhar
Alkhumra hemorrhagic fever virus (AHFV) is an emerging novel flavivirus that was discovered in Saudi Arabia in 1995. The virus has since caused several outbreaks in the country that resulted in case fatality rates ranging from 1% to 25%. Meager information has been published on the ultrastructural features of the virus on cells under in vitro or in vivo conditions. The present electron microscopic study examined and compared the intracellular growth of the AHFV on the LLC-MK2 cells and brain cells of new born Wistar rats, inoculated intracerebrally...
January 3, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29295909/lamp1-increases-the-efficiency-of-lassa-virus-infection-by-promoting-fusion-in-less-acidic-endosomal-compartments
#10
Christine E Hulseberg, Lucie Fénéant, Katarzyna M Szymańska, Judith M White
Lassa virus (LASV) is an arenavirus whose entry into host cells is mediated by a glycoprotein complex (GPC) comprised of a receptor binding subunit, GP1, a fusogenic transmembrane subunit, GP2, and a stable signal peptide. After receptor-mediated internalization, arenaviruses converge in the endocytic pathway, where they are thought to undergo low-pH-triggered, GPC-mediated fusion with a late endosome membrane. A unique feature of LASV entry is a pH-dependent switch from a primary cell surface receptor (α-dystroglycan) to an endosomal receptor, lysosomal-associated membrane protein (Lamp1)...
January 2, 2018: MBio
https://www.readbyqxmd.com/read/29291209/recent-advances-in-vaccine-development-against-ebola-threat-as-bioweapon
#11
EDITORIAL
Prachi Gera, Ankit Gupta, Priyanka Verma, Joginder Singh, Jeena Gupta
With the increasing rate of Ebola virus appearance, with multiple natural outbreaks of Ebola hemorrhagic fever, it is worthy of consideration as bioweapon by anti-national groups. Further, with the non-availability of the vaccines against Ebola virus, concerns about the public health emerge. In this regard, this review summarizes the structure, genetics and potential of Ebola virus to be used as a bioweapon. We highlight the recent advances in the treatment strategies and vaccine development against Ebola virus...
September 2017: Virusdisease
https://www.readbyqxmd.com/read/29285314/tlr2-myd88-pathway-dependent-regulation-of-dendritic-cells-by-dengue-virus-promotes-antibody-dependent-enhancement-via-th2-biased-immunity
#12
Junu Aleyas George, Seong Bum Kim, Jin Young Choi, Ajit Mahadev Patil, Ferdaus Mohd Altaf Hossain, Erdenebelig Uyangaa, Jin Hur, Sang-Youel Park, John-Hwa Lee, Koanhoi Kim, Seong Kug Eo
Possible risk mediators in primary dengue virus (DenV) infection that favor secondary DenV infection to life-threatening dengue hemorrhagic fever (DHF) and shock syndrome (DSS) via antibody-dependent enhancement (ADE) have not yet been described. Here, DenV infection enhanced the expression of inflammatory mediators and activation molecules in dendritic cells (DCs) through TLR2/MyD88 pathway. TLR2 appeared to facilitate DenV infection in DCs that were less permissive than macrophages for viral replication. In experiments using separate evaluations of DenV-infected and uninfected bystander DCs, infected DCs showed impaired maturation accompanied with TLR2-dependent production of inflammatory cytokines, by which uninfected bystander DCs showed increased expression of co-stimulatory molecules...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29281106/delayed-ifn-type-1-induced-antiviral-state-is-a-potential-factor-for-hfrs-severity
#13
Katarina Resman Rus, Miša Korva, Petra Bogovic, Emil Pal, Franc Strle, Tatjana Avšic-Županc
Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in Europe and Asia. Interferon (IFN) responses play an important role in HFRS pathogenesis and it is known that the early IFNβ response is delayed by pathogenic hantaviruses. The clinical severity of HFRS caused by Dobrava virus (DOBV) and Puumala virus (PUUV) varies greatly and the aim of our study was to determine whether differences in early activation of the IFN type 1-induced antiviral state influence HFRS severity. Peripheral blood mononuclear cells (PBMCs) from healthy donors and HFRS patients were stimulated with DOBV or PUUV and the expression of selected genes was measured...
December 21, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29274250/an-exosome-based-vaccine-platform-imparts-cytotoxic-t-lymphocyte-immunity-against-viral-antigens
#14
Simona Anticoli, Francesco Manfredi, Chiara Chiozzini, Claudia Arenaccio, Eleonora Olivetta, Flavia Ferrantelli, Antonio Capocefalo, Emiliana Falcone, Anna Ruggieri, Maurizio Federico
Exosomes are 50-150 nm sized nanovesicles released by all eukaryotic cells. We very recently described a method to engineer exosomes in vivo with the E7 protein of Human Papilloma Virus (HPV). This technique consists in the intramuscular injection of a DNA vector expressing HPV-E7 fused at the C-terminus of an exosome-anchoring protein, i.e., Nefmut , we previously characterized for its high levels of incorporation in exosomes. In this configuration, the ∼11 kDa E7 protein elicited a both strong and effective antigen-specific cytotoxic T lymphocyte (CTL) immunity...
December 23, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29273568/hantavirus-gc-induces-long-term-immune-protection-via-lamp-targeting-dna-vaccine-strategy
#15
Dong-Bo Jiang, Jin-Peng Zhang, Lin-Feng Cheng, Guan-Wen Zhang, Yun Li, Zi-Chao Li, Zhen-Hua Lu, Zi-Xin Zhang, Yu-Chen Lu, Lian-He Zheng, Fang-Lin Zhang, Kun Yang
Hemorrhagic fever with renal syndrome (HFRS) occurs widely throughout Eurasia. Unfortunately, there is no effective treatment, and prophylaxis remains the best option against the major pathogenic agent, hantaan virus (HTNV), which is an Old World hantavirus. However, the absence of cellular immune responses and immunological memory hampers acceptance of the current inactivated HFRS vaccine. Previous studies revealed that a lysosome-associated membrane protein 1 (LAMP1)-targeting strategy involving a DNA vaccine based on the HTNV glycoprotein Gn successfully conferred long-term immunity, and indicated that further research on Gc, another HTNV antigen, was warranted...
December 19, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29260328/vivo-morpholino-oligomers-strongly-inhibit-dengue-virus-replication-and-production
#16
Patta Phumesin, Mutita Junking, Aussara Panya, Petlada Yongpitakwattana, Sansanee Noisakran, Thawornchai Limjindaporn, Pa-Thai Yenchitsomanus
Dengue virus (DENV) infection is a worldwide public health problem, which can cause severe dengue hemorrhagic fever (DHF) and life-threatening dengue shock syndrome (DSS). There are currently no anti-DENV drugs available, and there has been an intensive search for effective anti-DENV agents that can inhibit all four DENV serotypes. In this study, we tested whether vivo-morpholino oligomers (vivo-MOs), whose effect on DENV infection has not previously been studied, can inhibit DENV infection. Vivo-MOs were designed to target the top of 3' stem-loop (3' SL) in the 3' UTR of the DENV genome and tested for inhibition of DENV infection in monkey kidney epithelial (Vero) cells and human lung epithelial carcinoma (A549) cells...
December 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29247706/episodes-of-the-epidemiological-factors-correlated-with-prevailing-viral-infections-with-dengue-virus-and-molecular-characterization-of-serotype-specific-dengue-virus-circulation-in-eastern-india
#17
M Rajesh Kumar Rao, Rabindra N Padhy, Manoj Kumar Das
BACKGROUND: Dengue is one of the most important and widespread viral infection comprises 4 related serotypes (DEN-1, 2, 3, and 4). Infection with one serotype does not protect against the others, and sequential infections put people at greater risk for dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). This study determines the epidemiology of prevailing viral infections with dengue and molecular characterization of serotype-specific DENV circulation in Odisha of eastern India...
December 13, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29233140/selection-characterization-and-thermal-stabilization-of-llama-single-domain-antibodies-towards-ebola-virus-glycoprotein
#18
Jinny L Liu, Lisa C Shriver-Lake, George P Anderson, Dan Zabetakis, Ellen R Goldman
BACKGROUND: A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for Ebola virus envelope glycoprotein (GP) and increased their stability to expand their utility for use in austere locals...
December 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29230614/translating-predictions-of-zoonotic-viruses-for-policymakers
#19
Seth D Judson, Matthew LeBreton, Trevon Fuller, Risa M Hoffman, Kevin Njabo, Timothy F Brewer, Elsa Dibongue, Joseph Diffo, Jean-Marc Feussom Kameni, Severin Loul, Godwin W Nchinda, Richard Njouom, Julius Nwobegahay, Jean Michel Takuo, Judith N Torimiro, Abel Wade, Thomas B Smith
Recent outbreaks of Ebola virus disease and Zika virus disease highlight the need for disseminating accurate predictions of emerging zoonotic viruses to national governments for disease surveillance and response. Although there are published maps for many emerging zoonotic viruses, it is unknown if there is agreement among different models or if they are concordant with national expert opinion. Therefore, we reviewed existing predictions for five high priority emerging zoonotic viruses with national experts in Cameroon to investigate these issues and determine how to make predictions more useful for national policymakers...
December 11, 2017: EcoHealth
https://www.readbyqxmd.com/read/29227420/us-state-public-health-departments-special-pathogen-planning
#20
Jocelyn J Herstein, Paul D Biddinger, Shawn G Gibbs, Aurora B Le, Katelyn C Jelden, Angela L Hewlett, John J Lowe
CONTEXT: US state public health departments played key roles in planning for and responding to confirmed and suspected cases of Ebola virus disease (EVD) during the 2014-2016 outbreak, including designating select hospitals as high-level isolation units (HLIUs) for EVD treatment in conjunction with the Centers for Disease Control and Prevention. OBJECTIVE: To identify existing guidelines and perspectives of state health departments pertaining to the management and transport of patients with EVD and other highly hazardous communicable diseases (HHCDs)...
December 7, 2017: Journal of Public Health Management and Practice: JPHMP
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