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Influenza microRNA

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https://www.readbyqxmd.com/read/28448456/upregulation-of-mirna-4776-in-influenza-virus-infected-bronchial-epithelial-cells-is-associated-with-downregulation-of-nfkbib-and-increased-viral-survival
#1
Sreekumar Othumpangat, Nicole B Bryan, Donald H Beezhold, John D Noti
Influenza A virus (IAV) infection remains a significant cause of morbidity and mortality worldwide. One key transcription factor that is activated upon IAV infection is nuclear factor Kappa B (NF-κB). NF-κB regulation involves the inhibitor proteins NF-κB inhibitor beta (NFKBIB), (also known as IκB β), which form complexes with NF-κB to sequester it in the cytoplasm. In this study, microarray data showed differential expression of several microRNAs (miRNAs) on exposure to IAV. Target scan analysis revealed that miR-4776, miR-4514 and miR-4742 potentially target NFKBIB messenger RNA (mRNA)...
April 27, 2017: Viruses
https://www.readbyqxmd.com/read/28405612/microrna-125a-and-b-inhibit-a20-and-mavs-to-promote-inflammation-and-impair-antiviral-response-in-copd
#2
Alan C-Y Hsu, Kamal Dua, Malcolm R Starkey, Tatt-Jhong Haw, Prema M Nair, Kristy Nichol, Nathan Zammit, Shane T Grey, Katherine J Baines, Paul S Foster, Philip M Hansbro, Peter A Wark
Influenza A virus (IAV) infections lead to severe inflammation in the airways. Patients with chronic obstructive pulmonary disease (COPD) characteristically have exaggerated airway inflammation and are more susceptible to infections with severe symptoms and increased mortality. The mechanisms that control inflammation during IAV infection and the mechanisms of immune dysregulation in COPD are unclear. We found that IAV infections lead to increased inflammatory and antiviral responses in primary bronchial epithelial cells (pBECs) from healthy nonsmoking and smoking subjects...
April 6, 2017: JCI Insight
https://www.readbyqxmd.com/read/28392443/enhanced-immunogenicity-following-mir-155-incorporation-into-the-influenza-a-virus-genome
#3
Leonard Izzard, Daniel Dlugolenski, Yingju Xia, Meagan McMahon, Deborah Middleton, Ralph A Tripp, John Stambas
Influenza A vaccine efficacy in the elderly is generally poor and so identification of novel molecular adjuvants to improve immunogenicity is important to reduce the overall burden of disease. Short non-coding RNAs, known as microRNAs (miRNAs) are known to regulate gene expression and have the potential to influence immune responses. One such miRNA, miR-155, has been shown to modulate T and B cell development and function. We incorporated miR-155 into the influenza A virus (IAV) genome creating a self-adjuvanting 'live vaccine' with the ability to modify immunogenicity...
April 7, 2017: Virus Research
https://www.readbyqxmd.com/read/28382020/h9n2-avian-influenza-virus-protein-pb1-enhances-the-immune-responses-of-bone-marrow-derived-dendritic-cells-by-down-regulating-mir375
#4
Jian Lin, Jing Xia, Chong Z Tu, Ke Y Zhang, Yan Zeng, Qian Yang
Polymerase basic protein 1 (PB1), the catalytic core of the influenza A virus RNA polymerase complex, is essential for viral transcription and replication. Dendritic cells (DCs) possess important antigen presenting ability and a crucial role in recognizing and clearing virus. MicroRNA (miRNA) influence the development of DCs and their ability to present antigens as well as the ability of avian influenza virus (AIV) to infect host cells and replicate. Here, we studied the molecular mechanism underlying the miRNA-mediated regulation of immune function in mouse DCs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28377766/host-genetic-background-strongly-affects-pulmonary-microrna-expression-before-and-during-influenza-a-virus-infection
#5
Matthias Preusse, Klaus Schughart, Frank Pessler
BACKGROUND: Expression of host microRNAs (miRNAs) changes markedly during influenza A virus (IAV) infection of natural and adaptive hosts, but their role in genetically determined host susceptibility to IAV infection has not been explored. We, therefore, compared pulmonary miRNA expression during IAV infection in two inbred mouse strains with differential susceptibility to IAV infection. RESULTS: miRNA expression profiles were determined in lungs of the more susceptible strain DBA/2J and the less susceptible strain C57BL/6J within 120 h post infection (hpi) with IAV (H1N1) PR8...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28368362/sus-scrofa-mir-204-and-mir-4331-negatively-regulate-swine-h1n1-2009-influenza-a-virus-replication-by-targeting-viral-ha-and-ns-respectively
#6
Shishuo Zhang, Ruifang Wang, Huijuan Su, Biaoxiong Wang, Suolang Sizhu, Zhixin Lei, Meilin Jin, Huanchun Chen, Jiyue Cao, Hongbo Zhou
The prevalence of swine pandemic H1N1/2009 influenza A virus (SIV-H1N1/2009) in pigs has the potential to generate novel reassortant viruses, posing a great threat to human health. Cellular microRNAs (miRNAs) have been proven as promising small molecules for regulating influenza A virus replication by directly targeting viral genomic RNA. In this study, we predicted potential Sus scrofa (ssc-, swine) miRNAs targeting the genomic RNA of SIV-H1N1/2009 by RegRNA 2.0, and identified ssc-miR-204 and ssc-miR-4331 to target viral HA and NS respectively through dual-luciferase reporter assays...
April 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28282445/endothelial-cell-tropism-is-a-determinant-of-h5n1-pathogenesis-in-mammalian-species
#7
Smanla Tundup, Matheswaran Kandasamy, Jasmine T Perez, Nacho Mena, John Steel, Tamas Nagy, Randy A Albrecht, Balaji Manicassamy
The cellular and molecular mechanisms underpinning the unusually high virulence of highly pathogenic avian influenza H5N1 viruses in mammalian species remains unknown. Here, we investigated if the cell tropism of H5N1 virus is a determinant of enhanced virulence in mammalian species. We engineered H5N1 viruses with restricted cell tropism through the exploitation of cell type-specific microRNA expression by incorporating microRNA target sites into the viral genome. Restriction of H5N1 replication in endothelial cells via miR-126 ameliorated disease symptoms, prevented systemic viral spread and limited mortality, despite showing similar levels of peak viral replication in the lungs as compared to control virus-infected mice...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28281253/microrna-biosensing-with-two-dimensional-surface-plasmon-resonance-imaging
#8
Ho Pui Ho, Fong Chuen Loo, Shu Yuen Wu, Dayong Gu, Ken-Tye Yong, Siu Kai Kong
Two-dimensional surface plasmon resonance (2D-SPR) imaging, which provides a real-time, sensitive, and high-throughput analysis of surface events in a two dimensional manner, is a valuable tool for studying biomolecular interactions and biochemical reactions without using any tag labels. The sensing principle of 2D-SPR includes angular, wavelength, and phase interrogation. In this chapter, the 2D-SPR imaging technique is applied for sensing a target microRNA by its corresponding oligonucleotide probes, with sequence complementarity, immobilized on the gold SPR sensing surface...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28244992/overcoming-cellular-barriers-for-rna-therapeutics
#9
Steven F Dowdy
RNA-based therapeutics, such as small-interfering (siRNAs), microRNAs (miRNAs), antisense oligonucleotides (ASOs), aptamers, synthetic mRNAs and CRISPR-Cas9, have great potential to target a large part of the currently undruggable genes and gene products and to generate entirely new therapeutic paradigms in disease, ranging from cancer to pandemic influenza to Alzheimer's disease. However, for these RNA modalities to reach their full potential, they first need to overcome a billion years of evolutionary defenses that have kept RNAs on the outside of cells from invading the inside of cells...
March 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28131813/microrna-146a-induction-during-influenza-h3n2-virus-infection-targets-and-regulates-traf6-levels-in-human-nasal-epithelial-cells-hnecs
#10
Yuqin Deng, Yan Yan, Kai Sen Tan, Jing Liu, Vincent T Chow, Ze-Zhang Tao, De-Yun Wang
We have previously shown that human nasal epithelial cells (hNECs) are highly permissive cells for respiratory viruses including influenza A virus (IAV) and respiratory syncytial virus. Recent studies have indicated that microRNAs (miRNAs) are involved in virus-host relationship, and this led us to investigate its essential roles in the in vitro hNECs model derived from multiple donors. By comparing the differential expression of miRNAs upon IAV infection among animal and cell line studies, candidates were selected with focus on the initial immune response...
March 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28063705/h9n2-avian-influenza-virus-enhances-the-immune-responses-of-bmdcs-by-down-regulating-mir29c
#11
Jian Lin, Jing Xia, Ya T Chen, Ke Y Zhang, Yan Zeng, Qian Yang
Avian influenza virus (AIV) of the subtypes H9 and N2 is well recognised and caused outbreaks-due to its high genetic variability and high rate of recombination with other influenza virus subtypes. The pathogenicity of H9N2 AIV depends on the host immune response. Dendritic cells (DCs) are major antigen presenting cells that can significantly inhibit H9N2 AIV replication. MicroRNAs (miRNAs) influence the ability of DCs to present antigens, as well as the ability of AIVs to infect host cells and replicate. Here, we studied the molecular mechanism underlying the miRNA-mediated regulation of immune function of mouse DCs...
February 1, 2017: Vaccine
https://www.readbyqxmd.com/read/28035991/involvement-of-host-non-coding-rnas-in-the-pathogenesis-of-the-influenza-virus
#12
REVIEW
Yanmei Ma, Jing Ouyang, Jingyun Wei, Mohamed Maarouf, Ji-Long Chen
Non-coding RNAs (ncRNAs) are a new type of regulators that play important roles in various cellular processes, including cell growth, differentiation, survival, and apoptosis. ncRNAs, including small non-coding RNAs (e.g., microRNAs, small interfering RNAs) and long non-coding RNAs (lncRNAs), are pervasively transcribed in human and mammalian cells. Recently, it has been recognized that these ncRNAs are critically implicated in the virus-host interaction as key regulators of transcription or post-transcription during viral infection...
December 27, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27922126/viral-and-bacterial-co-infection-in-severe-pneumonia-triggers-innate-immune-responses-and-specifically-enhances-ip-10-a-translational-study
#13
Jonathan Hoffmann, Daniela Machado, Olivier Terrier, Stephane Pouzol, Mélina Messaoudi, Wilma Basualdo, Emilio E Espínola, Rosa M Guillen, Manuel Rosa-Calatrava, Valentina Picot, Thomas Bénet, Hubert Endtz, Graciela Russomando, Gláucia Paranhos-Baccalà
Mixed viral and bacterial infections are widely described in community-acquired pneumonia; however, the clinical implications of co-infection on the associated immunopathology remain poorly studied. In this study, microRNA, mRNA and cytokine/chemokine secretion profiling were investigated for human monocyte-derived macrophages infected in-vitro with Influenza virus A/H1N1 and/or Streptococcus pneumoniae. We observed that the in-vitro co-infection synergistically increased interferon-γ-induced protein-10 (CXCL10, IP-10) expression compared to the singly-infected cells conditions...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27694730/the-microrna-let-7b-mediated-attenuated-strain-of-influenza-a-h1n1-virus-in-a-mouse-model
#14
Mingming Tan, Wenkui Sun, Chunlai Feng, Di Xia, Xiaoyue Shen, Yuan Ding, Zhicheng Liu, Zheng Xing, Xin Su, Yi Shi
INTRODUCTION: Evaluating the attenuation of influenza viruses in animal studies is important in developing safe and effective vaccines. This study aimed to demonstrate that the microRNA (miRNA)-let-7b-mediated attenuated influenza viruses (miRT-H1N1) are sufficiently attenuated and safe in mice. METHODOLOGY: The pathogenicity of the miRT-H1N1virus was investigated in a mouse model, evaluated with median lethal dose (LD50). The replicative dynamics of the miRT-H1N1, wild type (wt)-H1N1, and scramble (scbl)-H1N1 viruses in the lungs of infected mice were compared...
September 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27610823/microrna-34a-contributes-to-virus-mediated-apoptosis-through-binding-to-its-target-gene-bax-in-influenza-a-virus-infection
#15
Niannian Fan, Jiantao Wang
OBJECTIVE: Influenza A virus is a cytolytic virus that induces apoptosis in numerous cell types. However, molecular mechanism of cell apoptosis induced by influenza A virus is not well understood. The present study was aimed to investigate the molecular mechanism of miR-34a involved in influenza A virus induced apoptosis. METHODS: Firstly, microRNA array was used to select miRNAs involved in influenza A virus infection. Then, Luciferase reporter assay and western blot were carried to determine the target gene of miR-34a...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27597967/identification-of-dietetically-absorbed-rapeseed-brassica-campestris-l-bee-pollen-micrornas-in-serum-of-mice
#16
Xuan Chen, Guan-Hai Dai, Ze-Ming Ren, Ye-Ling Tong, Feng Yang, Yong-Qiang Zhu
MicroRNAs (miRNAs) are a class of small noncoding RNA that, through mediating posttranscriptional gene regulation, play a critical role in nearly all biological processes. Over the last decade it has become apparent that plant miRNAs may serve as a novel functional component of food with therapeutic effects including anti-influenza and antitumor. Rapeseed bee pollen has good properties in enhancing immune function as well as preventing and treating disease. In this study, we identified the exogenous miRNAs from rapeseed bee pollen in mice blood using RNA-seq technology...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27448447/microrna-21-drives-severe-steroid-insensitive-experimental-asthma-by-amplifying-phosphoinositide-3-kinase-mediated-suppression-of-histone-deacetylase-2
#17
Richard Y Kim, Jay C Horvat, James W Pinkerton, Malcolm R Starkey, Ama T Essilfie, Jemma R Mayall, Prema M Nair, Nicole G Hansbro, Bernadette Jones, Tatt Jhong Haw, Krishna P Sunkara, Thi Hiep Nguyen, Andrew G Jarnicki, Simon Keely, Joerg Mattes, Ian M Adcock, Paul S Foster, Philip M Hansbro
BACKGROUND: Severe steroid-insensitive asthma is a substantial clinical problem. Effective treatments are urgently required, however, their development is hampered by a lack of understanding of the mechanisms of disease pathogenesis. Steroid-insensitive asthma is associated with respiratory tract infections and noneosinophilic endotypes, including neutrophilic forms of disease. However, steroid-insensitive patients with eosinophil-enriched inflammation have also been described. The mechanisms that underpin infection-induced, severe steroid-insensitive asthma can be elucidated by using mouse models of disease...
February 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27322373/modulation-of-influenza-a-virus-replication-by-microrna-9-through-targeting-mcpip1
#18
Chunyan Dong, Xiaoning Sun, Zhenhong Guan, Maolin Zhang, Ming Duan
MicroRNAs (miRNAs), a family of small noncoding RNAs controlling translation and transcription of its target genes, play important roles in the regulation of various biological processes, including viral infection. Influenza A viruses (IAV) infection alters expression of cellular miRNAs, which in turn can modify the cellular environment to facilitate efficient virus replication. In this study, we showed that IAV infection significantly induced miR-9 expression in A549 cells, which occurred earlier than drastic expression of viral matrix (M) and nucleoprotein (NP) genes...
June 20, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27285980/mir674-inhibits-the-neuraminidase-stimulated-immune-response-on-dendritic-cells-via-down-regulated-mbnl3
#19
Jian Lin, Ya T Chen, Jing Xia, Qian Yang
Neuraminidase (NA), a structural protein of the H9N2 avian influenza virus (H9N2 AIV), can facilitate viral invasion of the upper airway by cleaving the sialic acid moieties on mucin. Dendritic cells (DCs) are major antigen-presenting cells whose immune functions, such as presenting antigens and activating lymphocytes, can be regulated by microRNAs. Here, we studied the molecular mechanism of miRNA-induced repression of immune responses in mouse DCs. First, we screened for and verified the miRNAs induced by NA...
August 2, 2016: Oncotarget
https://www.readbyqxmd.com/read/27166678/microrna-regulation-of-human-genes-essential-for-influenza-a-h7n9-replication
#20
Stefan Wolf, Weilin Wu, Cheryl Jones, Olivia Perwitasari, Suresh Mahalingam, Ralph A Tripp
Influenza A viruses are important pathogens of humans and animals. While seasonal influenza viruses infect humans every year, occasionally animal-origin viruses emerge to cause pandemics with significantly higher morbidity and mortality rates. In March 2013, the public health authorities of China reported three cases of laboratory confirmed human infection with avian influenza A (H7N9) virus, and subsequently there have been many cases reported across South East Asia and recently in North America. Most patients experience severe respiratory illness, and morbidity with mortality rates near 40%...
2016: PloS One
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