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https://www.readbyqxmd.com/read/28643888/combination-of-citrus-polymethoxyflavones-green-tea-polyphenols-and-lychee-extracts-suppresses-obesity-and-hepatic-steatosis-in-high-fat-diet-induced-obese-mice
#1
Min-Hsiung Pan, Guliang Yang, Shiming Li, Ming-Yi Li, Mei-Ling Tsai, Jia-Ching Wu, Vladimir Badmaev, Chi-Tang Ho, Ching-Shu Lai
SCOPE: SlimTrym(®) is a formulated product composed of citrus polymethoxyflavones (PMFs), green tea extract and lychee extract. We investigated the effect of dietary SlimTrym(®) on diet-induced obesity and associated non-alcoholic fatty liver disease (NAFLD) in mice. METHODS AND RESULTS: Male C57BL/6 mice were fed a normal diet (ND), high fat diet (HFD) or HFD containing 0.1% or 0.5% SlimTrym(®) for sixteen weeks. Dietary SlimTrym(®) significantly reduced weight gain and relative perigonadal, retroperitoneal, mesenteric fat weight as well as the size of adipocyte in HFD-fed mice...
June 23, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28640379/comparative-and-correlative-assessments-of-cytokine-complement-and-antibody-patterns-in-pediatric-type-1-diabetes
#2
M Abdel-Latif, A A Abdel-Moneim, M H El-Hefnawy, R G Khalil
One of the most widespread and effective environmental factors is the infection with enteroviruses (EVs) which accelerate β - cell destruction in type 1 diabetes (T1D). This study represented a comparison between diabetic EV(+) and EV- children as well as correlation analysis between autoantibodies, T1D markers, cytokines, complement activation products and anti- coxsackievirus (CV) IgG. EV RNA was detected in Egyptian children with T1D (26.2%) and healthy controls (0%). Detection of anti - CV IgG in T1D - EV(+) resulted in 64% positivity...
June 22, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28639122/targeting-prostate-specific-membrane-antigen-psma-with-f-18-labeled-compounds-the-influence-of-prosthetic-groups-on-tumor-uptake-and-clearance-profile
#3
Vincent Bouvet, Melinda Wuest, Justin J Bailey, Cody Bergman, Nancy Janzen, John F Valliant, Frank Wuest
PURPOSE: Prostate-specific membrane antigen (PSMA) is an important biomarker expressed in the majority of prostate cancers. The favorable positron emission tomography (PET) imaging profile of the PSMA imaging agent 2-(3-(1-carboxy-5-[(6-[(18)F]fluoro-pyridine-3-carbonyl)-amino]-pentyl)-ureido)-pentane-dioic acid [(18)F]DCFPyL in preclinical prostate cancer models and in prostate cancer patients stimulated the development and validation of other fluorine-containing PSMA inhibitors to further enhance pharmacokinetics and simplify production methods...
June 21, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28638398/discovery-of-putative-herbicide-resistance-genes-and-its-regulatory-network-in-chickpea-using-transcriptome-sequencing
#4
Mir A Iquebal, Khela R Soren, Priyanka Gangwar, P S Shanmugavadivel, K Aravind, Deepak Singla, Sarika Jaiswal, Rahul S Jasrotia, Sushil K Chaturvedi, Narendra P Singh, Rajeev K Varshney, Anil Rai, Dinesh Kumar
Background: Chickpea (Cicer arietinum L.) contributes 75% of total pulse production. Being cheaper than animal protein, makes it important in dietary requirement of developing countries. Weed not only competes with chickpea resulting into drastic yield reduction but also creates problem of harboring fungi, bacterial diseases and insect pests. Chemical approach having new herbicide discovery has constraint of limited lead molecule options, statutory regulations and environmental clearance. Through genetic approach, transgenic herbicide tolerant crop has given successful result but led to serious concern over ecological safety thus non-transgenic approach like marker assisted selection is desirable...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28631172/glutamine-a-major-player-in-nitrogen-catabolite-repression-in-the-yeast-dekkera-bruxellensis
#5
Danielli Batista Bezerra Cajueiro, Denise Castro Parente, Fernanda Cristina Bezerra Leite, Marcos Antonio de Morais Junior, Will de Barros Pita
In the present work we studied the expression of genes from nitrogen central metabolism in the yeast Dekkera bruxellensis and under regulation by the Nitrogen Catabolite Repression mechanism (NCR). These analyses could shed some light on the biological mechanisms involved in the adaptation and survival of this yeast in the sugarcane fermentation process for ethanol production. Nitrogen sources (N-sources) in the form of ammonium, nitrate, glutamate or glutamine were investigated with or without the addition of methionine sulfoximine, which inhibits the activity of the enzyme glutamine synthetase and releases cells from NCR...
June 19, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28630648/comparative-metabolic-profiling-of-the-lipid-producing-green-microalga-chlorella-reveals-that-nitrogen-and-carbon-metabolic-pathways-contribute-to-lipid-metabolism
#6
Hui Chen, Yanli Zheng, Jiao Zhan, Chenliu He, Qiang Wang
Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting conditions. However, the underlying mechanisms of lipid biosynthesis are unclear. In this study, we analyzed the global metabolic profiles of three genetically closely related Chlorella strains (C1, C2, and C3) with significant differences in lipid productivity to identify the contributions of key metabolic pathways to lipid metabolism...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28629519/erythropoietin-endogenous-protection-of-ischemic-brain
#7
Robert T Mallet, Myoung-Gwi Ryou
The human brain requires uninterrupted delivery of blood-borne oxygen and nutrients to sustain its function. Focal ischemia, particularly, ischemic stroke, and global ischemia imposed by cardiac arrest disrupt the brain's fuel supply. The resultant ATP depletion initiates a complex injury cascade encompassing intracellular Ca(2+) overload, glutamate excitotoxicity, oxido-nitrosative stress, extracellular matrix degradation, and inflammation, culminating in neuronal and astroglial necrosis and apoptosis, neurocognitive deficits, and even death...
2017: Vitamins and Hormones
https://www.readbyqxmd.com/read/28626421/divergent-metabolic-regulation-of-autophagy-and-mtorc1-early-events-in-alzheimer-s-disease
#8
REVIEW
Mai A Shafei, Matthew Harris, Myra E Conway
Alzheimer's disease (AD) is a progressive disease associated with the production and deposition of amyloid β-peptide (Aβ) aggregates and neurofibrillary tangles, which lead to synaptic and neuronal damage. Reduced autophagic flux has been widely associated with the accumulation of autophagic vacuoles (AV), which has been proposed to contribute to aggregate build-up observed in AD. As such, targeting autophagy regulation has received wide review, where an understanding as to how this mechanism can be controlled will be important to neuronal health...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28626198/the-therapeutic-effect-of-dexmedetomidine-on-rat-diabetic-neuropathy-pain-and-the-mechanism
#9
Yuecheng Lu, Baohua Lin, Junmin Zhong
Diabetic neuropathy pain (DNP) is a common chronic complication of diabetes characterized by spontaneous pain, hyperalgesia and allodynia. Dexmedetomidine is a selective α2 adrenergic agonist that relieves sympathetic nervous tension and reduces the release of glutamate. Thus, it is possible that dexmedetomidine may relieve DNP as well. In this study, we examined the effect of dexmedetomidine on DNP in the presence or absence of the α2 adrenergic antagonist yohimbine in rats utilizing a streptozotocin (STZ)-induced diabetes as a model of DNP...
June 14, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28625041/energy-regulation-in-newly-diagnosed-tb-with-chronic-energy-deficiency-free-fatty-acids-and-rbp4
#10
Nurpudji A Taslim, Devintha Virani, Ni Ketut Sumartini, Karmila, Agussalim Bukhari, Aminuddin, Suryani As'ad, Raden Satriono, Haerani Rasyid, Irawaty Djaharuddin
BACKGROUND AND OBJECTIVES: Energy metabolism may be dysfunctionally integral between host and infective agent in active tuberculosis, mediated by adipocytokines and free fatty acids (FFA) as the products of triglyceride lipolysis in fat, blood or other tissues. Retinol Binding Protein 4 (RBP4) and asymmetric dimethylarginine (ADMA) are candidate adipocytokines. The possibility of a deleterious metabolic nexus in chronic energy deficiency (CED) (BMI <18.5 kg/m2) is explored. METHODS AND DESIGN: Newly diagnosed patients with tuberculosis (n=63) were selected using consecutive random sampling at a Centre for the Care and Treatment of Lung Diseases in Makassar, Indonesia...
June 2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28622378/gene-modification-of-the-acetate-biosynthesis-pathway-in-escherichia-coli-and-implementation-of-the-cell-recycling-technology-to-increase-l-tryptophan-production
#11
Qingyang Xu, Fang Bai, Ning Chen, Gang Bai
The implementation of a novel cell recycling technology based on a special disk centrifuge during microbial fermentation process can continuously separate the product and harmful intermediates, while maintaining the cell viability owing to the installed cooling system. Acetate accumulation is an often encountered problem in L-tryptophan fermentation by Escherichia coli. To extend our previous studies, the current study deleted the key genes underlying acetate biosynthesis to improve l-tryptophan production...
2017: PloS One
https://www.readbyqxmd.com/read/28620442/efficacy-of-nanoceria-for-periodontal-tissues-alteration-in-glutamate-induced-obese-rats-multidisciplinary-considerations-for-personalized-dentistry-and-prevention
#12
Tetyana V Beregova, Karine S Neporada, Maksym Skrypnyk, Tetyana M Falalyeyeva, Nadiya M Zholobak, Oleksandr B Shcherbakov, Mykola Ya Spivak, Rostyslav V Bubnov
BACKGROUND: Nowadays, we face the global epidemic of obesity, that is known to contribute to the development of many diseases, such as the oral cavity pathologies. Dental and oral pathologies are frequently caused by and overlapped with systemic multifactorial diseases such as obesity being its early indicators and risk factors. The aim was to study the influence of nanoceria on periodontal tissues alteration in glutamate (MSG)-induced obese rats. METHODS: We included 52 Wistar rats of both genders and divided into four groups: newborn rats in group 1 (control) received subcutaneously 8 μl/g saline...
March 2017: EPMA Journal
https://www.readbyqxmd.com/read/28619110/effect-of-aspartic-acid-and-glutamate-on-metabolism-and-acid-stress-resistance-of-acetobacter-pasteurianus
#13
Haisong Yin, Renkuan Zhang, Menglei Xia, Xiaolei Bai, Jun Mou, Yu Zheng, Min Wang
BACKGROUND: Acetic acid bacteria (AAB) are widely applied in food, bioengineering and medicine fields. However, the acid stress at low pH conditions limits acetic acid fermentation efficiency and high concentration of vinegar production with AAB. Therefore, how to enhance resistance ability of the AAB remains as the major challenge. Amino acids play an important role in cell growth and cell survival under severe environment. However, until now the effects of amino acids on acetic fermentation and acid stress resistance of AAB have not been fully studied...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28616987/impact-of-low-intensity-pulsed-ultrasound-on-transcript-and-metabolite-abundance-in-saccharomyces-cerevisiae
#14
Michael C Schultz, Jian Zhang, Xian Luo, Oleksandra Savchenko, Liang Li, Michael Deyholos, Jie Chen
The interactions of ultrasound with biological materials are exploited for diagnostic, interventional and therapeutic multiple applications in medicine and can improve biological productivity in industrial biotechnological applications, such as biofuel generation, vaccine and antibodies production. Accordingly, there is great interest in better understanding the biological effects of ultrasound. In this paper, we studied the impact of low-intensity pulsed ultrasound (LIPUS) on RNA expression and metabolism of S...
June 15, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28614662/investigating-liquid-liquid-phase-separation-of-a-monoclonal-antibody-using-solution-state-nmr-spectroscopy-effect-of-arg%C3%A2-glu-and-arg%C3%A2-hcl
#15
Priscilla Kheddo, Jack E Bramham, Rebecca J Dearman, Shahid Uddin, Christopher F van der Walle, Alexander P Golovanov
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense (protein-rich) and diluted (protein-lean) phases, and should be avoided in the final drug product. Understanding the factors leading to LLPS, and ways to predict and prevent it would therefore be highly beneficial. Here we describe the link between LLPS behavior of an IgG1 mAb ('mAb5'), its solubility and parameters extracted using (1)H NMR spectroscopy, for various formulations. We show that the formulations demonstrating least LLPS lead to the largest mAb5 NMR signal intensities...
June 14, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28611499/lactobacillus-futsaii-cs3-a-new-gaba-producing-strain-isolated-from-thai-fermented-shrimp-kung-som
#16
Chatthaphisuth Sanchart, Onnicha Rattanaporn, Dietmar Haltrich, Pimpimol Phukpattaranont, Suppasil Maneerat
Kung-Som is a popular traditional Thai fermented shrimp product. It is rich in glutamic acid, which is the major substrate for the biosynthesis of gamma-aminobutyric acid (GABA) by lactic acid bacteria (LAB). In the present study, LAB from Kung-Som were isolated, screened for GABA formation, and the two isolates that transform glutamic acid most efficiently into GABA were identified. Based on the API-CHL50 fermentation profile and a phylogenetic tree of 16S rDNA sequences, strain CS3 and CS5 were identified as Lactobacillus futsaii, which was for the first time shown to be a promising GABA producer...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28609101/design-synthesis-and-evaluation-of-thiazolidine-2-4-dione-derivatives-as-a-novel-class-of-glutaminase-inhibitors
#17
Teng-Kuang Yeh, Ching-Chuan Kuo, Yue-Zhi Lee, Yi-Yu Ke, Kuang-Feng Chu, Hsing-Yu Hsu, Hsin-Yu Chang, Yu-Wei Liu, Jen-Shin Song, Cheng-Wei Yang, Li-Mei Lin, Manwu Sun, Szu-Huei Wu, Po-Chu Kuo, Chuan Shih, Chiung-Tong Chen, Lun Kelvin Tsou, Shiow-Ju Lee
Humans have two glutaminase genes, GLS (GLS1) and GLS2, each of which has two alternative transcripts: the kidney isoform (KGA) and glutaminase C (GAC) for GLS; and the liver isoform (LGA) and glutaminase B (GAB) for GLS2. Initial hit compound (Z)-5-((1-(4-bromophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)methylene)thiazolidine-2,4-dione (1), a thiazolidine-2-4-dione, was obtained from a high throughput screening of 40,000 compounds against KGA. Subsequently, a series of thiazolidine-2-4-dione derivatives was synthesized...
June 13, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28606738/engineering-high-level-production-of-fatty-alcohols-by-saccharomyces-cerevisiae-from-lignocellulosic-feedstocks
#18
Leo d'Espaux, Amit Ghosh, Weerawat Runguphan, Maren Wehrs, Feng Xu, Oliver Konzock, Ishaan Dev, Melissa Nhan, Jennifer Gin, Amanda Reider Apel, Christopher J Petzold, Seema Singh, Blake A Simmons, Aindrila Mukhopadhyay, Héctor García Martín, Jay D Keasling
Fatty alcohols in the C12-C18 range are used in personal care products, lubricants, and potentially biofuels. These compounds can be produced from the fatty acid pathway by a fatty acid reductase (FAR), yet yields from the preferred industrial host Saccharomyces cerevisiae remain under 2% of the theoretical maximum from glucose. Here we improved titer and yield of fatty alcohols using an approach involving quantitative analysis of protein levels and metabolic flux, engineering enzyme level and localization, pull-push-block engineering of carbon flux, and cofactor balancing...
June 10, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28605892/improvement-of-thermal-stability-of-bcg-vaccine
#19
Fatemeh Jahanbakhsh Sefidi, Homan Kaghazian, Gholam Ali Moradli, Seyed Mehdi Hassanzadeh
BAckground: Thermal stability (TS) is a part of the BCG vaccine characterization by which consistency of process in BCG vaccine production could be confirmed. To enhance the TS of the vaccine, some prevalent stabilizers in different concentration were added to the final formulation of BCG bulk prior to Freeze-drying process. We found a formulation more effective than the current stabilizer for retaining the higher viability of lyophilized BCG vaccine produced by Pasteur Institute of Iran...
June 13, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28603695/the-pag-gene-of-pxo1-is-involved-in-capsule-biosynthesis-of-bacillus-anthracis-pasteur-ii-strain
#20
Xudong Liang, Jin Zhu, Zhongzhi Zhao, Feng Zheng, Huijuan Zhang, Jianchun Wei, Yon Ji, Yinduo Ji
The poly-γ-D-glutamic acid capsule and anthrax toxins are major virulence factors of Bacillus anthracis. Genes responsible for capsule biosynthesis are located on pXO2, whereas genes encoding the toxins, which are composed of edema factors, lethal factors, and protective antigens (PA), are located on pXO1. In this study, we found that the pag null mutation not only eliminated the production of the protective antigen, it also eliminated the ability of the B. anthracis Pasteur II strain to form capsules. qPCR analysis revealed that the deletion of pag decreased the transcription levels of the capABCD operon and its regulatory genes acpA and acpB...
2017: Frontiers in Cellular and Infection Microbiology
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