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https://www.readbyqxmd.com/read/28804485/transcriptional-profiling-of-midguts-prepared-from-trypanosoma-t-congolense-positive-glossina-palpalis-palpalis-collected-from-two-distinct-cameroonian-foci-coordinated-signatures-of-the-midguts-remodeling-as-t-congolense-supportive-niches
#1
Jean M Tsagmo Ngoune, Flobert Njiokou, Béatrice Loriod, Ginette Kame-Ngasse, Nicolas Fernandez-Nunez, Claire Rioualen, Jacques van Helden, Anne Geiger
Our previous transcriptomic analysis of Glossina palpalis gambiensis experimentally infected or not with Trypanosoma brucei gambiense aimed to detect differentially expressed genes (DEGs) associated with infection. Specifically, we selected candidate genes governing tsetse fly vector competence that could be used in the context of an anti-vector strategy, to control human and/or animal trypanosomiasis. The present study aimed to verify whether gene expression in field tsetse flies (G. p. palpalis) is modified in response to natural infection by trypanosomes (T...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28803999/growth-arrest-and-morphological-changes-triggered-by-emodin-on-trypanosoma-cruzi-epimastigotes-cultivated-in-axenic-medium
#2
Ana R De Lima, Karem Noris-Suárez, Antonio Bretaña, Victor T Contreras, Maria C Navarro, Luis Pérez-Ybarra, José Bubis
Emodin is an anthraquinone obtained from Rheum palmatum rootstocks. Here we tested the cytotoxic effects of emodin on Trypanosoma cruzi epimastigotes, as well as the morphological changes that were induced by this compound in the parasite. Emodin was permeable and blocked in vitro cell division of T. cruzi epimastigotes in axenic medium, causing growth arrest in a dose-dependent but reversible manner. Emodin-exposed epimastigotes underwent duplication of organelles, such as the nucleus, kinetoplast and flagellum, but were incapable of completing cytokinesis...
August 10, 2017: Biochimie
https://www.readbyqxmd.com/read/28802260/the-essential-functions-of-krepb4-are-developmentally-distinct-and-required-for-endonuclease-association-with-editosomes
#3
Suzanne M McDermott, Kenneth Stuart
Uridine insertion and deletion RNA editing generates functional mitochondrial mRNAs in Trypanosoma brucei, and several transcripts are differentially edited in bloodstream (BF) and procyclic form (PF) cells correlating with changes in mitochondrial function. Editing is catalyzed by three ∼20S editosomes that have a common set of 12 proteins, but are typified by mutually exclusive RNase III KREN1, N2, and N3 endonucleases with distinct cleavage specificities. KREPB4 is a common editosome protein that has a degenerate RNase III domain lacking conserved catalytic residues, in addition to zinc-finger and Pumilio/fem-3 mRNA binding factor (PUF) motifs...
August 11, 2017: RNA
https://www.readbyqxmd.com/read/28801098/synthesis-and-in-vitro-evaluation-of-ca-2-channel-blockers-1-4-dihydropyridines-analogues-against-trypanosoma-cruzi-and-leishmania-amazonensis-sar-analysis
#4
Luiz A E Pollo, Milene H de Moraes, Júlia Cisilotto, Tânia B Creczynski-Pasa, Maique W Biavatti, Mario Steindel, Louis P Sandjo
Drugs containing the1,4-dihydropyridine (DHP) core have recently attracted attention concerning their antiparasitic effect against various species of Leishmania and Trypanosoma. This approach named drugs repositioning led to interesting results, which have prompted us to prepare 21 DHP's analogues. The 1,4-DHP scaffold was decorated with different function groups at tree points including the nitrogen atom (NH and N-phenyl), the aryl group attached to C-4 (various substituted aryl residues) and the carbon atoms 2 and 6 (bearing Ph or Me groups)...
August 8, 2017: Parasitology International
https://www.readbyqxmd.com/read/28800609/the-trypomastigote-small-surface-antigen-tssa-regulates-trypanosoma-cruzi-infectivity-and-differentiation
#5
María de Los Milagros Cámara, Gaspar E Cánepa, Andrés B Lantos, Virginia Balouz, Hai Yu, Xi Chen, Oscar Campetella, Juan Mucci, Carlos A Buscaglia
BACKGROUND: TSSA (Trypomastigote Small Surface Antigen) is an antigenic, adhesion molecule displayed on the surface of Trypanosoma cruzi trypomastigotes. TSSA displays substantial sequence identity to members of the TcMUC gene family, which code for the trypomastigote mucins (tGPI-mucins). In addition, TSSA bears sequence polymorphisms among parasite strains; and two TSSA variants expressed as recombinant molecules (termed TSSA-CL and TSSA-Sy) were shown to exhibit contrasting features in their host cell binding and signaling properties...
August 11, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28800584/expression-of-the-rna-binding-protein-rbp10-promotes-the-bloodstream-form-differentiation-state-in-trypanosoma-brucei
#6
Elisha Mugo, Christine Clayton
In nearly all eukaryotes, cellular differentiation is governed by changes in transcription, and stabilized by chromatin and DNA modification. Gene expression control in the pathogen Trypanosoma brucei, in contrast, relies almost exclusively on post-transcriptional mechanisms, so RNA binding proteins must assume the burden that is usually borne by transcription factors. T. brucei multiply in the blood of mammals as bloodstream forms, and in the midgut of Tsetse flies as procyclic forms. We show here that a single RNA-binding protein, RBP10, promotes the bloodstream-form trypanosome differentiation state...
August 11, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28795372/development-of-a-pharmacophore-for-cruzain-using-oxadiazoles-as-virtual-molecular-probes-quantitative-structure-activity-relationship-studies
#7
Anacleto S de Souza, Marcelo T de Oliveira, Adriano D Andricopulo
Chagas's is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi. According to the World Health Organization, 7 million people are infected worldwide leading to 7000 deaths per year. Drugs available, nifurtimox and benzimidazole, are limited due to low efficacy and high toxicity. As a validated target, cruzain represents a major front in drug discovery attempts for Chagas disease. Herein, we describe the development of 2D QSAR ([Formula: see text] = 0.81) and a 3D-QSAR-based pharmacophore ([Formula: see text] = 0...
August 9, 2017: Journal of Computer-aided Molecular Design
https://www.readbyqxmd.com/read/28793017/enveloped-and-non-enveloped-viral-like-particles-in-trypanosoma-cruzi-epimastigotes
#8
Ana María Fernández-Presas, Luis Padilla-Noriega, Ingeborg Becker, Lilia Robert, José Agustín Jiménez, Sandra Solano, Jose Delgado, Patricia Tato, José Luis Molinari
Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed...
August 7, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28792096/the-inositol-pyrophosphate-synthesis-pathway-in-trypanosoma-brucei-is-linked-to-polyphosphate-synthesis-in-acidocalcisomes
#9
Ciro D Cordeiro, Adolfo Saiardi, Roberto Docampo
Inositol pyrophosphates are novel signaling molecules possessing high-energy pyrophosphate bonds and involved in a number of biological functions. Here, we report the correct identification and characterization of the kinases involved in the inositol pyrophosphate biosynthetic pathway in Trypanosoma brucei: inositol polyphosphate multikinase (TbIPMK), inositol pentakisphosphate 2-kinase (TbIP5K) and inositol hexakisphosphate kinase (TbIP6K). TbIP5K and TbIP6K were not identifiable by sequence alone and their activities were validated by enzymatic assays with the recombinant proteins or by their complementation of yeast mutants...
August 9, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28789893/lead-selection-of-antiparasitic-compounds-from-a-focused-library-of-benzenesulfonyl-derivatives-of-heterocycles
#10
Romina J Pagliero, Marcel Kaiser, Reto Brun, Marcelo J Nieto, María R Mazzieri
A library of 89 synthetic benzenesulfonyl derivatives of heterocycles with drug-like properties was assayed for in vitro antiparasitic activity and the results were added to our previously reported derivatives for a comprehensive SAR evaluation. Four compounds showed an IC50 between 0.25 and 3μM against Leishmania donovani and low cytotoxicity. Compound G{16} (1-(2,3,5,6-tetramethylphenylsulfonyl)-2-methylindoline), was particularly interesting with an IC50 similar to the reference drug miltefosine. Seven compounds showed an IC50 below 6µM against Trypanosoma cruzi, and three of them (E{3}, E{9} and G{3}) were identified as lead scaffolds for further optimization based on their activity-toxicity profile...
July 31, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28789716/in-vitro-antileishmanial-activity-and-iron-superoxide-dismutase-inhibition-of-arylamine-mannich-base-derivatives
#11
Alvaro Martin-Montes, Mery Santivañez-Veliz, Elsa Moreno-Viguri, Rubén Martín-Escolano, Carmen Jiménez-Montes, Catalina Lopez-Gonzalez, Clotilde Marín, Carmen Sanmartín, Ramón Gutiérrez Sánchez, Manuel Sánchez-Moreno, Silvia Pérez-Silanes
Leishmaniasis is one of the world's most neglected diseases, and it has a worldwide prevalence of 12 million. There are no effective human vaccines for its prevention, and treatment is hampered by outdated drugs. Therefore, research aiming at the development of new therapeutic tools to fight leishmaniasis remains a crucial goal today. With this purpose in mind, we present 20 arylaminoketone derivatives with a very interesting in vitro and in vivo efficacy against Trypanosoma cruzi that have now been studied against promastigote and amastigote forms of Leishmania infantum, Leishmania donovani and Leishmania braziliensis strains...
August 9, 2017: Parasitology
https://www.readbyqxmd.com/read/28783594/antigenic-characterization-of-52-55kda-protein-isolated-from-trypanosoma-evansi-and-its-application-in-detection-of-equine-trypanosomosis
#12
S C Yadav, Ritesh Kumar, Jaideep Kumar, Meetali Singh, B C Bera, Rajender Kumar, U Tatu, Kanika Tehri
Trypanosoma evansi is a haemo-protozoan parasite responsible for the disease surra, an economically important disease of wide range of domestic and wild animals. The present diagnostic methods using soluble antigens have inherent problems like lack of standardized and reproducible antigens, as well as ethical issues. This entails further efforts for search of defined antigenic molecules with satisfying sensitivity and specificity for sero-epidemiology of trypanosomosis. In present investigation, we have identified and purified 52-55kDa immuno-dominant protein cluster in molecular mass ranges by preparatory SDS-PAGE methods from T...
August 1, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28777208/survey-of-pediatric-infectious-diseases-society-members-about-congenital-chagas-disease
#13
Morven S Edwards, Francisca A Abanyie, Susan P Montgomery
Participants in a survey about congenital Chagas disease, distributed electronically to Pediatric Infectious Diseases Society members, perceived having limited knowledge about congenital Trypanosoma cruzi infection. Most rarely or never consider the diagnosis in infants born to parents from Latin America. Improved awareness of congenital Chagas disease and assessment of at-risk infants is needed.
August 1, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28774654/chagas-disease-importance-of-rats-as-reservoir-hosts-of-trypanosoma-cruzi-chagas-1909-in-western-mexico
#14
Gumercindo Goicochea-Del Rosal, Benjamín Nogueda-Torres, María Elena Villagrán, José Antonio de Diego-Cabrera, Oziel Dante Montañez-Valdez, José Alejandro Martínez-Ibarra
In Mexico, the role of most species of mammals involved in the transmission cycle of Trypanosoma cruzi Chagas, 1909 is poorly known. It was carried out a study to investigate the importance of rats as reservoir of T. cruzi in western Mexico, an area with important risk of transmission of T. cruzi to human. Thirty-eight human dwellings were searched on two representative towns of western Mexico along twelve months for collection of rats and triatomines. Study rats (Rattus norvegicus) Berkenhout, 1769 and triatomines (Meccus phyllosomus longipennis) (Usinger, 1939) were collected inside and outside human dwellings...
July 31, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28769980/galectin-3-knockdown-impairs-survival-migration-and-immunomodulatory-actions-of-mesenchymal-stromal-cells-in-a-mouse-model-of-chagas-disease-cardiomyopathy
#15
Bruno Solano de Freitas Souza, Kátia Nunes da Silva, Daniela Nascimento Silva, Vinícius Pinto Costa Rocha, Bruno Diaz Paredes, Carine Machado Azevedo, Carolina Kymie Nonaka, Gisele Batista Carvalho, Juliana Fraga Vasconcelos, Ricardo Ribeiro Dos Santos, Milena Botelho Pereira Soares
Therapies based on transplantation of mesenchymal stromal cells (MSC) hold promise for the management of inflammatory disorders. In chronic Chagas disease cardiomyopathy (CCC), caused by chronic infection with Trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modulated by MSC. Here, we investigated the role of galectin-3 (Gal-3), a beta-galactoside-binding lectin with several actions on immune responses and repair process, on the immunomodulatory potential of MSC...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28769924/thymic-stromal-lymphopoietin-is-critical-for-regulation-of-proinflammatory-cytokine-response-and-resistance-to-experimental-trypanosoma-congolense-infection
#16
Chukwunonso Onyilagha, Rani Singh, Abdelilah Soussi Gounni, Jude Ezeh Uzonna
African trypanosomiasis (sleeping sickness) poses serious threat to human and animal health in sub-Saharan Africa. Because there is currently no vaccine for preventing this disease and available drugs are not safe, understanding the mechanisms that regulate resistance and/or susceptibility to the disease could reveal novel targets for effective disease therapy and prevention. Thymic stromal lymphopoietin (TSLP) plays a critical role in driving Th2 immune response. Although susceptibility to experimental Trypanosoma congolense infection in mice is associated with excessive proinflammatory responses due in part to impaired Th2 response, the role of TSLP in resistance to African trypanosomiasis has not been well studied...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28768559/maldi-tof-ms-protein-profiling-for-the-rapid-identification-of-chagas-disease-triatomine-vectors-and-application-to-the-triatomine-fauna-of-french-guiana
#17
Maureen Laroche, Jean-Michel Bérenger, Gladys Gazelle, Denis Blanchet, Didier Raoult, Philippe Parola
Triatomines are haematophagous insects involved in the transmission of Trypanosoma cruzi, the aetiological agent of Chagas disease. The vector competence of these arthropods can be highly variable, depending on the species. A precise identification is therefore crucial for the epidemiological surveillance of T. cruzi and the determination of at-risk human populations. To circumvent the difficulties of morphological identification and the lack of comprehensiveness of the GenBank database, we hereby propose an alternative method for triatomine identification...
August 3, 2017: Parasitology
https://www.readbyqxmd.com/read/28767983/evidence-for-regulated-expression-of-telomeric-repeat-containing-rnas-terra-in-parasitic-trypanosomatids
#18
Jeziel D Damasceno, Gabriel LA Silva, Christian Tschudi, Luiz Ro Tosi
The Telomeric Repeat-containing RNAs (TERRA) participate in the homeostasis of telomeres in higher eukaryotes. Here, we investigated the expression of TERRA in Leishmania spp. and Trypanosoma brucei and found evidences for its expression as a specific RNA class. The trypanosomatid TERRA are heterogeneous in size and partially polyadenylated. The levels of TERRA transcripts appear to be modulated through the life cycle in both trypanosomatids investigated, suggesting that TERRA play a stage-specific role in the life cycle of these early-branching eukaryotes...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767982/description-of-an-oral-chagas-disease-outbreak-in-venezuela-including-a-vertically-transmitted-case
#19
Belkisyolé Alarcón de Noya, Gladymar Pérez-Chacón, Zoraida Díaz-Bello, Sonia Dickson, Arturo Muñoz-Calderón, Carlos Hernández, Yadira Pérez, Luciano Mauriello, Eyleen Moronta
We describe the eleventh major outbreak of foodborne Trypanosoma cruzi transmission in urban Venezuela, including evidence for vertical transmission from the index case to her fetus. After confirming fetal death at 24 weeks of gestation, pregnancy interruption was performed. On direct examination of the amniotic fluid, trypomastigotes were detected. T. cruzi specific-polymerase chain reaction (PCR) also proved positive when examining autopsied fetal organs. Finally, microscopic fetal heart examination revealed amastigote nests...
August 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28767980/bone-marrow-cell-migration-to-the-heart-in-a-chimeric-mouse-model-of-acute-chagasic-disease
#20
Camila Iansen Irion, Bruno Diaz Paredes, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, Luis Felipe Paula, Alysson Roncally Carvalho, Antonio Carlos Campos de Carvalho, Adriana Bastos Carvalho, Regina Coeli Dos Santos Goldenberg
BACKGROUND: Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare...
August 2017: Memórias do Instituto Oswaldo Cruz
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