keyword
MENU ▼
Read by QxMD icon Read
search

trypanosoma

keyword
https://www.readbyqxmd.com/read/29785922/karyotype-evolution-of-chagas-disease-vectors-hemiptera-triatominae
#1
Kaio Cesar Chaboli Alevi, Jader de Oliveira, João Aristeu da Rosa, Maria Tercília Vilela de Azeredo-Oliveira
The Triatominae subfamily is composed of 153 hematophagous species that are potential vectors of Trypanosoma cruzi , the etiological agent of Chagas disease. Karyotypic studies in triatomines were initiated in 1909. There are 92 karyotypes described, all grouped into the tribes Rhodniini and Triatomini. Recently, a phylogenetic study of the triatomines that combines molecular data with geological changes was performed. We now discuss how the karyotype evolved with the diversification of the triatomines.
May 21, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29785042/inducible-high-efficiency-crispr-cas9-targeted-gene-editing-and-precision-base-editing-in-african-trypanosomes
#2
Eva Rico, Laura Jeacock, Julie Kovářová, David Horn
The Cas9 endonuclease can be programmed by guide RNA to introduce sequence-specific breaks in genomic DNA. Thus, Cas9-based approaches present a range of novel options for genome manipulation and precision editing. African trypanosomes are parasites that cause lethal human and animal diseases. They also serve as models for studies on eukaryotic biology, including 'divergent' biology. Genome modification, exploiting the native homologous recombination machinery, has been important for studies on trypanosomes but often requires multiple rounds of transfection using selectable markers that integrate at low efficiency...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29783099/leveraging-the-cruzain-s3-subsite-to-increase-affinity-for-reversible-covalent-inhibitors
#3
Lorenzo Cianni, Geraldo Sartori, Fabiana Rosini, Daniela De Vita, Gabriel Pires, Bianca Rebelo Lopes, Andrei Leitão, Antonio C B Burtoloso, Carlos A Montanari
Cruzain is the major cysteine protease of Trypanosoma cruzi, the etiological agent of Chagas disease. Reversible covalent cruzain inhibitors can block the steps of cell differentiation in the parasite and kill the organism. To this end, the description of how inhibitors modified at the P2/P3 positions lead to analogs with greater cruzain affinity to the S2/S3 subsites is of fundamental importance. Albeit many efforts are being employed in the characterization of the interaction processes with S2 subsite, little is known about the cruzain S3 subsite...
April 26, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29782493/dissecting-the-phyloepidemiology-of-trypanosoma-cruzi-i-tci-in-brazil-by-the-use-of-high-resolution-genetic-markers
#4
Fabiola Roman, Samanta das Chagas Xavier, Louisa A Messenger, Márcio G Pavan, Michael A Miles, Ana María Jansen, Matthew Yeo
BACKGROUND: Trypanosoma cruzi, the causal agent of Chagas disease, is monophyletic but genetically heterogeneous. It is currently represented by six genetic lineages (Discrete Typing Units, DTUs) designated TcI-TcVI. TcI is the most geographically widespread and genetically heterogeneous lineage, this as is evidenced by a wide range of genetic markers applied to isolates spanning a vast geographic range in Latin America. METHODOLOGY/PRINCIPAL FINDINGS: In total, 78 TcI isolated from hosts and vectors distributed in 5 different biomes of Brazil, were analyzed using 6 nuclear housekeeping genes, 25 microsatellite loci and one mitochondrial marker...
May 21, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29781553/effects-of-melatonin-on-thymic-and-oxidative-stress-dysfunctions-during-trypanosoma-cruzi-infection
#5
Vânia Brazão, Rafaela Pravato Colato, Fabricia Helena Santello, Gabriel Tavares do Vale, Natália de Almeida Gonzaga, Carlos Renato Tirapelli, José Clóvis do Prado
Although the exact etiology of Chagas' disease is not completely elucidated, thymic atrophy and oxidative stress are believed to be important contributors to the pathogenesis during acute Trypanosoma cruzi (T. cruzi) infection. We hypothethized that exogenous melatonin, administered by gavage (5 mg/kg, p.o., gavage) to young (5 weeks old) and middle-aged (18 months old) male Wistar rats would modulate thymic oxidative damage and reverse the age-related thymus regression during T. cruzi acute infection. Increased levels of superoxide anion (O2 - ) were detected in the thymus of infected animals and treatment with melatonin reverted this response...
May 21, 2018: Journal of Pineal Research
https://www.readbyqxmd.com/read/29781112/identification-of-toefaz1-interacting-proteins-reveals-key-regulators-of-trypanosoma-brucei-cytokinesis
#6
Nicholas A Hilton, Thomas E Sladewski, Jenna A Perry, Zemplen Pataki, Amy N Sinclair-Davis, Richard S Muniz, Holly L Tran, Jenna I Wurster, Jiwon Seo, Christopher L de Graffenried
The protist parasite Trypanosoma brucei is an obligate extracellular pathogen that retains its highly-polarized morphology during cell division and has evolved a novel cytokinetic process independent of non-muscle myosin II. The polo-like kinase homolog TbPLK is essential for transmission of cell polarity during division and for cytokinesis. We previously identified a putative TbPLK substrate named Tip of the Extending FAZ 1 (TOEFAZ1) as an essential kinetoplastid-specific component of the T. brucei cytokinetic machinery...
May 21, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29779491/trypanosoma-brucei-rhodesiense-infection-in-a-chinese-traveler-returning-from-the-serengeti-national-park-in-tanzania
#7
Qin Liu, Xiao-Ling Chen, Mu-Xin Chen, Han-Guo Xie, Qing Liu, Zhu-Yun Chen, Yao-Ying Lin, Hua Zheng, Jia-Xu Chen, Yi Zhang, Xiao-Nong Zhou
BACKGROUND: Human African trypanosomiasis (HAT) is one of the most complex parasitic diseases known to humankind. It usually occurs in endemic areas in Africa, but is occasionally detected in returning travelers and migrants in non-endemic countries. CASE PRESENTATION: In August 2017, a case of HAT was diagnosed in China in a traveler returning from the Masai Mara area in Kenya and the Serengeti area in Tanzania. The traveler visited Africa from 23 July to 5 August, 2017...
May 21, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29778893/synthesis-of-proline-derived-benzenesulfonamides-a-potent-anti-trypanosoma-brucei-gambiense-agent
#8
David I Ugwu, Uchechukwu C Okoro, Narendra K Mishra
Thousands of death in Africa and other developing nations are still attributed to trypanosomiasis. Excessive sleep has been associated with increased inflammation. We report herein, the synthesis, antitrypanosomal and anti-inflammatory activities of eight new carboxamide derivatives bearing substituted benzenesulfonamides. The base promoted reactions of l-proline and L-4-hydroxyproline with substituted benzenesulfonyl chlorides gave the benzenesulfonamides (11a-h) in excellent yields. Boric acid mediated amidation of the benzenesulfonamides (11a-h) and p-aminobenzoic acid (12) gave the new carboxamides (13a-h) in excellent yields...
May 14, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29778466/in-vitro-susceptibility-of-trypanosoma-brucei-brucei-to-selected-essential-oils-and-their-major-components
#9
Sonya Costa, Cláudia Cavadas, Carlos Cavaleiro, Lígia Salgueiro, Maria do Céu Sousa
Aiming for discovering effective and harmless antitrypanosomal agents, 17 essential oils and nine major components were screened for their effects on T. b. brucei. The essential oils were obtained by hydrodistillation from fresh plant material and analyzed by GC and GC-MS. The trypanocidal activity was assessed using blood stream trypomastigotes cultures of T. b. brucei and the colorimetric resazurin method. The MTT test was used to assess the cytotoxicity of essential oils on macrophage cells and Selectivity Indexes were calculated...
May 17, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29778329/the-kinetoplast-dna-of-the-australian-trypanosome-trypanosoma-copemani-shares-features-with-trypanosoma-cruzi-and-trypanosoma-lewisi
#10
Adriana Botero, Irit Kapeller, Crystal Cooper, Peta L Clode, Joseph Shlomai, R C Andrew Thompson
Kinetoplast DNA (kDNA) is the mitochondrial genome of trypanosomatids. It consists of a few dozen maxicircles and several thousand minicircles, all catenated topologically to form a two-dimensional DNA network. Minicircles are heterogeneous in size and sequence among species. They present one or several conserved regions that contain three highly conserved sequence blocks (CSBs). CSB-1 (10 bp sequence) and CSB-2 (8 bp sequence) present lower interspecies homology, while CSB-3 (12 bp sequence) or the Universal Minicircle Sequence (UMS) is conserved within most trypanosomatids...
May 17, 2018: International Journal for Parasitology
https://www.readbyqxmd.com/read/29775566/the-challenge-of-discordant-serology-in-chagas-disease-the-role-of-two-confirmatory-techniques-in-inconclusive-cases
#11
Zaira Moure, Elena Sulleiro, Laura Iniesta, Carmen Guillen, Israel Molina, M Magdalena Alcover, Cristina Riera, Tomás Pumarola, Roser Fisa
Serodiscordance in Chagas disease (CD) remains a challenge since individuals with inconclusive results are clinically complicated to manage. This work, conducted outside the endemic area, aims to compare two different confirmatory techniques for the diagnosis of CD in individuals without a definitive diagnosis, to analyze the performance of the screening techniques in this group of patients, and to describe the serological follow-up of these subjects over time. Sera from 48 individuals with repeatedly discordant results by one recombinant enzyme immunoassay (r-ELISA) and one native ELISA (n-ELISA), were included in the study...
May 15, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29774028/nlrp3-inflammasome-and-caspase-1-11-pathway-orchestrate-different-outcomes-in-the-host-protection-against-trypanosoma-cruzi-acute-infection
#12
Augusto F Paroli, Patricia V Gonzalez, Cintia Díaz-Luján, Luisina I Onofrio, Alfredo Arocena, Roxana C Cano, Eugenio A Carrera-Silva, Susana Gea
Infection with protozoan parasite Trypanosoma cruzi results in activation of nucleotide-binding domain and leucine-rich repeat containing receptors (NLRs). NLR activation leads to inflammasome formation, the activation of caspase-1, and the subsequent cleavage of IL-1β and IL-18. Considering that inflammasome activation and IL-1β induction by macrophages are key players for an appropriate T cell response, we investigated the relevance of NLR pyrin domain-containing 3 (NLRP3) and caspase-1/11 to elucidate their roles in the induction of different T cell phenotypes and the relationship with parasite load and hepatic inflammation during T...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29772025/shape-shifting-trypanosomes-flagellar-shortening-followed-by-asymmetric-division-in-trypanosoma-congolense-from-the-tsetse-proventriculus
#13
Lori Peacock, Christopher Kay, Mick Bailey, Wendy Gibson
Trypanosomatids such as Leishmania and Trypanosoma are digenetic, single-celled, parasitic flagellates that undergo complex life cycles involving morphological and metabolic changes to fit them for survival in different environments within their mammalian and insect hosts. According to current consensus, asymmetric division enables trypanosomatids to achieve the major morphological rearrangements associated with transition between developmental stages. Contrary to this view, here we show that the African trypanosome Trypanosoma congolense, an important livestock pathogen, undergoes extensive cell remodelling, involving shortening of the cell body and flagellum, during its transition from free-swimming proventricular forms to attached epimastigotes in vitro...
May 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29771525/an-nmr-biochemical-assay-for-oxidosqualene-cyclase-evaluation-of-inhibitor-activities-on-trypanosoma-cruzi-and-human-enzymes
#14
Osamu Tani, Yukie Akutsu, Shinji Ito, Takayuki Suzuki, Yukihiro Tateishi, Tomohiko Yamaguchi, Tatsuya Niimi, Ichiji Namatame, Yasunori Chiba, Hitoshi Sakashita, Tomomi Kubota, Tetsuo Yanagi, Shusaku Mizukami, Kenji Hirayama, Koji Furukawa, Kazuhiko Yamasaki
Oxidosqualene cyclase (OSC), a membrane-associated protein, is a key enzyme of sterol biosynthesis. Here we report a novel assay for OSC, involving reaction in aqueous solution, NMR quantification in organic solvent, and factor analysis of spectra. We evaluated one known and three novel inhibitors on OSC of Trypanosoma cruzi, a parasite causative of Chagas disease, and compared with their effects on human OSC for selectivity. Among them, one novel inhibitor showed a significant parasiticidal activity.
May 17, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29768603/-satisfaction-of-chagas-disease-patients-attended-at-a-pharmaceutical-care-service-in-the-state-of-cear%C3%A3-brazil
#15
Alanna Carla da Costa, Darlan da Silva Cândido, Arduina Sofia Ortet de Barros Vasconcelos Fidalgo, José Damião da Silva Filho, Carlos Eduardo Menezes Viana, Monise Anne Lima, Mônica Coelho Andrade, Maria de Fátima Oliveira
In 2005, a pharmaceutical care service was created in the State of Ceará to provide pharmacotherapeutic follow-up for individuals infected with Trypanosoma cruzi (Chagas Disease). After 10 years of operation, an evaluation was conducted to assess the degree of satisfaction of patients treated under the service. This prospective study used a questionnaire comprising the following sections: socioeconomic data; infrastructure, facilities and operations; pharmaceutical care; and importance of the service. Seventy patients of both sexes and over 18 years of age were interviewed between August 2014 and May 2015...
May 2018: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/29768552/natural-infection-by-trypanosoma-cruzi-in-triatomines-and-seropositivity-for-chagas-disease-of-dogs-in-rural-areas-of-rio-grande-do-norte-brazil
#16
Yannara Barbosa Nogueira Freitas, Celeste da Silva Freitas de Souza, Jamille Maia E Magalhães, Maressa Laíse Reginaldo de Sousa, Luiz Ney d'Escoffier, Tânia Zaverucha do Valle, Teresa Cristina Monte Gonçalves, Hélcio Reinaldo Gil-Santana, Thais Aaparecida Kazimoto, Sthenia Santos Albano Amora
INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and identify T. cruzi infection in triatomines, as well as to assess the presence of anti-T. cruzi antibodies in dogs in rural areas of the municipality of Mossoró, Brazil. METHODS: An active entomologic research was conducted to identify adult specimens through an external morphology dichotomous key...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29768550/insect-vectors-of-chagas-disease-trypanosoma-cruzi-in-northeastern-brazil
#17
Arduina Sofia Ortet de Barros Vasconcelos Fidalgo, Alanna Carla da Costa, José Damião da Silva Filho, Darlan da Silva Cândido, Erlane Chaves Freitas, Laíse Dos Santos Pereira, Mônica Coelho de Andrade, Kátia Cristina Morais Soares Gomes, Cláudia Mendonça Bezerra, Maria de Fátima Oliveira
INTRODUCTION: Chagas disease remains a public health problem in the rural and urban areas of 19 countries in the Americas. METHODS: The aim of the present study was to investigate the Trypanosoma cruzi infection rate of triatomines collected from both intra- and peridomiciliary areas in eleven municipalities of Southeastern Ceará, Brazil, from 2009 to 2015. RESULTS: A total of 32,364 triatomine specimens, including nymphs and adults, were collected, and 31,736 (98...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29768499/a-fixable-probe-for-visualizing-flagella-and-plasma-membranes-of-the-african-trypanosome
#18
Justin Wiedeman, Kojo Mensa-Wilmot
The protozoan Trypanosoma brucei sp. cause diseases in humans and animals. Studies of T. brucei cell biology have revealed unique features, such as major endocytic events being limited to a single region, and mitochondrial genome segregation mediated via basal bodies. Further understanding of trypanosome cell biology can be facilitated with super-resolution fluorescence microscopy. Lack of a plasma membrane probe for fixed trypanosomes remains a persistent problem in need of a working solution. Herein, we report protocols developed using mCLING in super-resolution structured illumination fluorescence microscopy (SR-SIM)...
2018: PloS One
https://www.readbyqxmd.com/read/29768103/efficacy-of-recombinase-polymerase-amplification-to-diagnose-trypanosoma-cruzi-infection-in-dogs-with-cardiac-alterations-from-an-endemic-area-of-mexico
#19
Matilde Jimenez-Coello, Thomas Shelite, Alejandro Castellanos-Gonzalez, Omar Saldarriaga, Rocio Rivero, Antonio Ortega-Pacheco, Carlos Acevedo-Arcique, Karla Amaya-Guardia, Nisha Garg, Peter Melby, Bruno L Travi
Chagas disease is a lingering Public Health problem in Latin America with ∼5.7 million people infected with Trypanosoma cruzi. Transmission is still taking place in most countries of the Americas, including the United States. Dogs are frequently infected with T. cruzi and its high infection prevalence is associated with increased risk of Chagas disease in humans. The city of Mérida in the Yucatan peninsula is endemic for Chagas disease and canines are frequently infected with T. cruzi. The objective of this study was to evaluate the performance of a qualitative point of care (POC) molecular test (RPA-LF, recombinase polymerase amplification-lateral flow) developed in our laboratory for identifying infected dogs...
May 16, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29764941/a-trypanosome-specific-protein-cooperates-with-the-cif1-protein-to-promote-cytokinesis-in-trypanosoma-brucei
#20
Yasuhiro Kurasawa, Huiqing Hu, Qing Zhou, Ziyin Li
Cytokinesis, the terminal step in cell division, in the protist human pathogen Trypanosoma brucei occurs along the longitudinal axis from the anterior tip of the new flagellum attachment zone (FAZ) towards the posterior cell tip. This process is regulated by a signaling cascade composed of the Polo-like kinase homolog TbPLK, the Aurora B kinase homolog TbAUK1, and the trypanosome-specific CIF1-CIF2 protein complex. However, the regulatory mechanism and the signaling pathway for this unusual mode of cytokinesis remain poorly understood...
May 15, 2018: Journal of Biological Chemistry
keyword
keyword
4103
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"