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https://www.readbyqxmd.com/read/28213174/evidence-of-the-presence-of-a-calmodulin-sensitive-plasma-membrane-ca-2-atpase-in-trypanosoma-equiperdum
#1
María Carolina Pérez-Gordones, José Rubén Ramírez-Iglesias, Vincenza Cervino, Graciela L Uzcanga, Gustavo Benaim, Marta Mendoza
Trypanosoma equiperdum belongs to the subgenus Trypanozoon, which has a significant socio-economic impact by limiting animal protein productivity worldwide. Proteins involved in the intracellular Ca(2+) regulation are prospective chemotherapeutic targets since several drugs used in experimental treatment against trypanosomatids exert their action through the disruption of the parasite intracellular Ca(2+) homeostasis. Therefore, the plasma membrane Ca(2+)-ATPase (PMCA) is considered as a potential drug target...
February 14, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28212935/dissecting-biochemical-peculiarities-of-the-atpase-activity-of-tcsub2-a-component-of-the-mrna-export-pathway-in-trypanosoma-cruzi
#2
Ize de Aguiar Bittencourt, Mariana Serpeloni, Priscila Mazzochi Hiraiwa, Tatiana de Arruda Campos Brasil de Souza, Andréa Rodrigues Ávila
The RNA helicase DEAD-box protein Sub2 (yeast)/UAP56 (mammals) is conserved across eukaryotes and is essential for mRNA export in trypanosomes. Despite the high conservation of Sub2 in lower eukaryotes such as Trypanosoma cruzi, the low conservation of other mRNA export factors raises questions regarding whether the mode of action of TcSub2 is similar to that of orthologs from other eukaryotes. Mutation of the conserved K87 residue of TcSub2 abolishes ATPase activity, showing that its ATPase domain is functional...
February 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28212811/polymorphisms-of-blood-forms-and-in-vitro-metacyclogenesis-of-trypanosoma-cruzi-i-ii-and-iv
#3
Camila Piva Abegg, Ana Paula de Abreu, Juliane Lopes da Silva, Silvana Marques de Araújo, Mônica Lúcia Gomes, Érika Cristina Ferreira, Max Jean de Ornelas Toledo
Trypanosoma cruzi is the etiologic agent of American trypanosomiasis has broad biological and genetic diversity. Remaining to be studied are polymorphisms of the blood forms and metacyclogenesis of different T. cruzi discrete typing units (DTUs). Our goal was to evaluate the relationship between T. cruzi DTUs, the morphology of blood trypomastigotes, and in vitro metacyclogenesis. T. cruzi strains that pertained to DTUs TcI, TcII, and TcIV from different Brazilian states were used. Parameters that were related to the morphology of eight strains were assessed in thin blood smears that were obtained from mice that were inoculated with blood or culture forms, depending on strain...
February 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28207833/structure-kinetic-characterization-and-subcellular-localization-of-the-two-ribulose-5-phosphate-epimerase-isoenzymes-from-trypanosoma-cruzi
#4
Soledad Natalia Gonzalez, Wanda Mariela Valsecchi, Dante Maugeri, José María Delfino, Juan José Cazzulo
The enzyme of the pentose phosphate pathway (PPP) ribulose-5-phosphate-epimerase (RPE) is encoded by two genes present in the genome of Trypanosoma cruzi CL Brener clone: TcRPE1 and TcRPE2. Despite high sequence similarity at the amino acid residue level, the recombinant isoenzymes show a strikingly different kinetics. Whereas TcRPE2 follows a typical michaelian behavior, TcRPE1 shows a complex kinetic pattern, displaying a biphasic curve, suggesting the coexistence of -at least- two kinetically different molecular forms...
2017: PloS One
https://www.readbyqxmd.com/read/28204532/characterization-of-the-single-subunit-oligosaccharyltransferase-stt3a-from-trypanosoma-brucei-using-synthetic-peptides-andlipid-linked-oligosaccharide-analogs
#5
Ana S Ramírez, Jérémy Boilevin, Rasomoy Biswas, Bee Ha Gan, Daniel Janser, Markus Aebi, Tamis Darbre, Jean-Louis Reymond, Kaspar P Locher
No abstract text is available yet for this article.
February 15, 2017: Glycobiology
https://www.readbyqxmd.com/read/28203518/inositol-1-4-5-trisphosphate-receptor-determines-intracellular-ca-2-concentration-in-trypanosoma-cruzi-throughout-its-life-cycle
#6
Muneaki Hashimoto, Motomichi Doi, Nagomi Kurebayashi, Koji Furukawa, Hiroko Hirawake-Mogi, Yoshihiro Ohmiya, Takashi Sakurai, Toshihiro Mita, Katsuhiko Mikoshiba, Takeshi Nara
Regulation of intracellular Ca(2+) concentration ([Ca(2+)]i) is vital for eukaryotic organisms. Recently, we identified a Ca(2+) channel (TcIP 3R) associated with intracellular Ca(2+) stores in Trypanosoma cruzi, the parasitic protist that causes Chagas disease. In this study, we measured [Ca(2+)]i during the parasite life cycle and determined whether TcIP 3R is involved in the observed variations. Parasites expressing R-GECO1, a red fluorescent, genetically encoded Ca(2+) indicator for optical imaging that fluoresces when bound to Ca(2+), were produced...
December 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/28202792/towards-a-new-strategy-for-the-diagnosis-of-congenital-trypanosoma-cruzi-infection
#7
Alba Abras, Carmen Muñoz, Cristina Ballart, Pere Berenguer, Teresa Llovet, Mercedes Herrero, Silvia Tebar, María-Jesús Pinazo, Elizabeth Posada, Carmen Martí, Victoria Fumadó, Jordi Bosch, Oriol Coll, Teresa Juncosa, Gemma Ginovart, Josep Armengol, Joaquim Gascón, Montserrat Portús, Montserrat Gállego
The immigration of Latin American women of childbearing age has spread the congenital transmission of Chagas disease to non-endemic areas, and the disease is now a worldwide problem. Some European health authorities have implemented screening programs to prevent vertical transmission, but the lack of a uniform protocol calls for the urgent establishment of a new strategy, common for all laboratories. Our aims were (i) to analyze the trend of passive IgG antibodies in the newborn by means of five serological tests for the diagnosis and follow-up of congenital Trypanosoma cruzi infection, (ii) to assess the utility of these techniques for diagnosing a congenital transmission, and (iii) to propose a strategy for a prompt, efficient and cost-effective diagnosis of T...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28201741/treatment-of-infected-women-of-childbearing-age-prevents-congenital-trypanosoma-cruzi-infection-by-eliminating-the-parasitemia-detected-by-pcr
#8
Laura Murcia, Marina Simón, Bartolomé Carrilero, Mercedes Roig, Manuel Segovia
No abstract text is available yet for this article.
February 14, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28199333/comparative-and-functional-triatomine-genomics-reveals-reductions-and-expansions-in-insecticide-resistance-related-gene-families
#9
Lucila Traverso, Andrés Lavore, Ivana Sierra, Victorio Palacio, Jesús Martinez-Barnetche, José Manuel Latorre-Estivalis, Gaston Mougabure-Cueto, Flavio Francini, Marcelo G Lorenzo, Mario Henry Rodríguez, Sheila Ons, Rolando V Rivera-Pomar
BACKGROUND: Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas' disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. Therefore, the incorporation of strategies for resistance management is a main concern for vector control programs...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28198632/engineering-oral-and-parenteral-amorphous-amphotericin-b-formulations-against-experimental-trypanosoma-cruzi-infections
#10
Miriam Rolón, Dolores R Serrano, Aikaterini Lalatsa, Esther de Pablo, Juan Jose Torrado, Maria Paloma Ballesteros, Anne Marie Healy, Celeste Vega, Cathia Coronel, Francisco Bolas-Fernández, Maria Auxiliadora Dea-Ayuela
Chagas disease (CD) is a parasitic zoonosis endemic in most mainland countries of Central and South America affecting nearly 10 million people, with 100 million people at high risk of contracting the disease. Treatment is only effective if received at the early stages of the disease. Only two drugs (benznidazole and nifurtimox) have so far been marketed and both share various limitations such as variable efficacy, many side effects and long duration of treatment, thus reducing compliance. The in vitro and in vivo efficacy of poly-aggregated amphotericin B (AmB), encapsulated poly-aggregated AmB in albumin microspheres (AmB-AME) and dimeric AmB - sodium deoxycholate micelles (AmB-NaDC) was evaluated...
February 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28194625/trypanosomiasis-challenge-estimation-using-the-diminazene-aceturate-berenil-index-in-zebu-in-gabon
#11
Brieuc G A Cossic, Brice Adjahoutonon, Pierre Gloaguen, Gui Lov Dibanganga, Gael Maganga, Pascal Leroy, Ewan T MacLeod, Kim Picozzi
A longitudinal study was conducted within a cattle ranch in Gabon to determine the diminazene aceturate (Berenil) index (DAI) in a group of Zebu, raised under low tsetse density; this measure providing an assessment of trypanosomiasis risk. The objective was to evaluate the trypanosomiasis pressure thus informing trypanosomiasis control methods and cattle management. Twenty female adult Zebu were monitored for 24 weeks during the dry season. Blood samples were collected on a weekly basis and subjected to parasitological and haematological analysis (n = 480), using the buffy-coat method and the packed cell volume value (PCV), respectively, infected animals were treated with a single intramuscular injection of diminazene aceturate (8 mg/kg)...
February 14, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28193733/giant-faz10-is-required-for-flagellum-attachment-zone-stabilization-and-furrow-positioning-in-trypanosoma-brucei
#12
Bernardo Pereira Moreira, Carol Kobori Da Fonseca, Tansy C Hammarton, Munira Muhammad Abdel Baqui
The flagellum and flagellum attachment zone (FAZ) are important cytoskeletal structures in trypanosomatids, being required for motility, cell division and cell morphogenesis. Trypanosomatid cytoskeletons contain abundant high molecular weight proteins (HMWPs), but many of their biological functions are still unclear. Here, we report the characterization of the giant FAZ protein, FAZ10, in Trypanosoma brucei, which we show using immuno-electron microscopy, localises to the intermembrane staples in the FAZ intracellular domain...
February 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28193658/in-vitro-ex-vivo-and-in-vivo-activity-of-diamidines-against-trypanosoma-congolense-and-trypanosoma-vivax
#13
Kirsten Gillingwater, Christina Kunz, Christiane Braghiroli, David W Boykin, Richard R Tidwell, Reto Brun
African Animal Trypanosomosis (AAT) is caused by the tsetse-transmitted protozoans, Trypanosoma congolense and T. vivax, leading to huge agricultural losses throughout sub-Saharan Africa. Three drugs are available to treat Nagana in cattle (diminazene diaceturate, homidium- and isometamidium chloride). With increasing reports of drug-resistant populations, new molecules should be investigated as potential candidates to combat Nagana. Dicationic compounds have demonstrated excellent efficacy against different kinetoplastid parasites...
February 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28192425/improving-access-to-chagas-disease-diagnosis-and-etiologic-treatment-in-remote-rural-communities-of-the-argentine-chaco-through-strengthened-primary-health-care-and-broad-social-participation
#14
Paula Sartor, Ivana Colaianni, M Victoria Cardinal, Jacqueline Bua, Héctor Freilij, Ricardo E Gürtler
BACKGROUND: Rural populations in the Gran Chaco region have large prevalence rates of Trypanosoma cruzi infection and very limited access to diagnosis and treatment. We implemented an innovative strategy to bridge these gaps in 13 rural villages of Pampa del Indio held under sustained vector surveillance and control. METHODOLOGY: The non-randomized treatment program included participatory workshops, capacity strengthening of local health personnel, serodiagnosis, qualitative and quantitative PCRs, a 60-day treatment course with benznidazole and follow-up...
February 13, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28192210/phylogenomics-of-trypanosoma-cruzi-few-evidence-of-tci-tcii-mosaicism-in-tciii-challenges-the-hypothesis-of-an-ancient-tci-tcii-hybridization
#15
Nicolás Tomasini, Patricio Diosque
Phylogenetic relationships among major lineages of Trypanosoma cruzi are still debatable. Particularly, it is controversial the origin of two main lineages: TcIII and TcIV. Some authors proposed that these lineages have been the result of an ancient hybridization between TcI and TcII, and this was one of the most accepted evolutionary models in the scientific community for several years. In the present paper we analyse several genomes of T. cruzi in order to examine if there is evidence supporting that TcIII is an ancient TcI/TcII hybrid...
February 10, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28189085/synthesis-and-antitrypanosomal-activities-of-novel-pyridylchalcones
#16
Avninder S Bhambra, Ketan C Ruparelia, Hoon L Tan, Deniz Tasdemir, Hollie Burrell-Saward, Vanessa Yardley, Kenneth J M Beresford, Randolph R J Arroo
A library of novel pyridylchalcones were synthesised and screened against Trypanosoma brucei rhodesiense. Eight were shown to have good activity with the most potent 8 having an IC50 value of 0.29 μM. Cytotoxicity testing with human KB cells showed a good selectivity profile for this compound with a selectivity index of 47. Little activity was seen when the library was tested against Leishmania donovani. In conclusion, pyridylchalcones are promising leads in the development of novel compounds for the treatment of human African trypanosomiasis (HAT)...
January 23, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28189084/synthesis-and-trypanocidal-activity-of-a-library-of-4-substituted-2-1h-pyrrolo-3-2-c-pyridin-2-yl-propan-2-ols
#17
Michael N Balfour, Caio H Franco, Carolina B Moraes, Lucio H Freitas-Junior, Hélio A Stefani
A library of 16 4-substituted 2-(1H-pyrrolo[3,2-c]pyridin-2-yl)propan-2-ols 17-32 has been synthesized for use in biological testing against Trypanosoma cruzi, the protozoan parasite that causes Chagas disease. The 4-substituted 2-(1H-pyrrolo[3,2-c]pyridin-2-yl)propan-2-ols 17-32 were subjected to biological testing to evaluate their efficacy against intracellular Trypanosoma cruzi (Y strain) amastigotes infecting U2OS human cells, with benznidazole as a reference compound. The assay was performed in duplicate (two independent experiments) and submitted to High Content Analysis (HCA) for determination of trypanocidal activity...
February 3, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28187957/discovery-of-n-2-aminoethyl-n-benzyloxyphenyl-benzamides-new-potent-trypanosoma-brucei-inhibitors
#18
Andriy Buchynskyy, J Robert Gillespie, Matthew A Hulverson, Joshua McQueen, Sharon A Creason, Ranae M Ranade, Nicole A Duster, Michael H Gelb, Frederick S Buckner
A phenotypic screen of a compound library for antiparasitic activity on Trypanosoma brucei, the causative agent of Human African Trypanosomiasis (HAT), led to the identification of N-(2-aminoethyl)-N-phenyl benzamides as a starting point for hit-to-lead medicinal chemistry. Eighty two analogues were prepared, which led to the identification of a set of highly potent N-(2-aminoethyl)-N-benzyloxyphenyl benzamides with the most potent compound 73 having an in vitro EC50=0.001μM. The compounds displayed drug-like properties when tested in a number of in vitro assays...
November 12, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28185987/real-time-pcr-strategy-for-the-identification-of-trypanosoma-cruzi-discrete-typing-units-directly-in-chronically-infected-human-blood
#19
Catalina Muñoz-San Martín, Werner Apt, Inés Zulantay
The protozoan Trypanosoma cruzi is the causative agent of Chagas disease, a major public health problem in Latin America. This parasite has a complex population structure comprised by six or seven major evolutionary lineages (discrete typing units or DTUs) TcI-TcVI and TcBat, some of which have apparently resulted from ancient hybridization events. Because of the existence of significant biological differences between these lineages, strain characterization methods have been essential to study T. cruzi in its different vectors and hosts...
February 6, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28185949/achyrocline-satureioides-essential-oil-loaded-in-nanocapsules-ameliorate-the-antioxidant-oxidant-status-in-heart-of-rats-infected-with-trypanosoma-evansi
#20
Carine F Souza, Matheus D Baldissera, Luciana F Cossetin, Daiane F Dalla Lana, Silvia G Monteiro
Oxidative stress has been considered as a pathological mechanism that contributes to initiation and progression of cardiac injury during the Trypanosoma evansi infection. In this sense, the natural compounds with antioxidant and free radical scavenger abilities, such the Achyrocline satureioides essential oil loaded in nanocapsules (AS-NC), may be considered important approach to minimize the cardiac damage. Thus, the aim of this study was to investigate whether AS-NC treatment is able to prevents or reduce the cardiac oxidative damage in infected rats with T...
February 6, 2017: Microbial Pathogenesis
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