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Amnion epithelial cells

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https://www.readbyqxmd.com/read/27907214/derivation-of-induced-trophoblast-cell-lines-in-cattle-by-doxycycline-inducible-piggybac-vectors
#1
Takamasa Kawaguchi, Dooseon Cho, Masafumi Hayashi, Tomoyuki Tsukiyama, Koji Kimura, Shuichi Matsuyama, Naojiro Minami, Masayasu Yamada, Hiroshi Imai
Trophectoderm lineage specification is one of the earliest differentiation events in mammalian development. The trophoblast lineage, which is derived from the trophectoderm, mediates implantation and placental formation. However, the processes involved in trophoblastic differentiation and placental formation in cattle remain unclear due to interspecies differences when compared with other model systems and the small repertoire of available trophoblast cell lines. Here, we describe the generation of trophoblast cell lines (biTBCs) from bovine amnion-derived cells (bADCs) using an induced pluripotent stem cell technique...
2016: PloS One
https://www.readbyqxmd.com/read/27904034/regulation-of-matrix-metalloproteinases-2-and-9-gene-expression-in-cultured-human-fetal-membrane-cells-by-influenza-virus-infection
#2
Noboru Uchide, Kyoko Obatake, Rie Yamada, Hidetaka Sadanari, Keiko Matsubara, Tsugiya Murayama, Kunio Ohyama
In order to understand a possible etiology of adverse pregnancy outcomes associated with intrauterine influenza virus infection, we examined the effect of influenza virus infection on gene expression of matrix metalloproteinases (MMPs) in cultured amnion epithelial, amnion mesenchymal and chorion trophoblast cells prepared from human fetal membrane tissues by gelatin zymography, Western blotting and reverse transcriptase-PCR. The cells were infected with influenza A (H1N1) virus. The levels of pro-MMP-9 activity in culture supernatants of three types of cells were increased during the period of 24-48 h after the virus infection as compared to those of mock infection...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27895585/feto-maternal-trafficking-of-exosomes-in-murine-pregnancy-models
#3
Samantha Sheller-Miller, Jun Lei, George Saade, Carlos Salomon, Irina Burd, Ramkumar Menon
Timing and initiation of labor are well-orchestrated by signals communicated between the fetal and maternal compartments; however, how these signals are communicated is not completely understood. Fetal exosomes, intercellular signaling vesicles, may play a key role in the process. The objective of this study was to evaluate exosome trafficking in vivo from fetal to maternal compartments. Pregnant CD-1 mice were intra-amniotically injected on gestational day 16 and 17 with exosomes isolated from primary human amnion epithelial cells fluorescently labeled with the lipophilic dye 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR)...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#4
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27852013/modulation-of-cell-attachment-proliferation-and-angiogenesis-by-decellularized-dehydrated-human-amniotic-membrane-in-in-vitro-models
#5
Xuan Guo, Aleksandr Kaplunovsky, Raihana Zaka, Chuan Wang, Hemlata Rana, Jeffrey Turner, Qian Ye, Ivana Djuretic, Joseph Gleason, Vladimir Jankovic, Janice M Smiell, Mohit Bhatia, Wolfgang Hofgartner, Robert Hariri
BACKGROUND: Decellularized, dehydrated human amniotic membrane (DDHAM) is an extracellular matrix devoid of cells, cell debris, and growth factors. This study examines the effect of cell attachment to the DDHAM and the induced cellular responses. MATERIALS AND METHODS: The cell types employed in this study were human dermal fibroblasts (HDF), human epithelial keratinocytes (HEK), and human dermal microvascular endothelial cells (HDMEC), all of which play critical roles in the wound healing process...
October 24, 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/27834419/limitations-and-options-using-resorbable-versus-nonresorbable-membranes-for-successful-guided-bone-regeneration
#6
Nikolaos K Soldatos, Popi Stylianou, Vasiliki P Koidou, Nikola Angelov, Raymond Yukna, Georgios E Romanos
OBJECTIVE: Deficient bony ridges often complicate the implant treatment plan. Several treatment modalities are used to regenerate bone, including guided bone regeneration (GBR). The purpose of this study was to summarize the knowledge on different types of membranes available and currently used in GBR procedures in a staged approach or with simultaneous implant placement. The primary role of the membranes is to exclude epithelial and connective tissue cells from the wound area to be regenerated, and to create and maintain the space into which pluripotential and osteogenic cells are free to migrate...
November 10, 2016: Quintessence International
https://www.readbyqxmd.com/read/27821556/amnion-epithelial-cells-promote-lung-repair-via-lipoxin-a4
#7
Jean L Tan, Yan Z Tan, Ruth Muljadi, Siow T Chan, Sin N Lau, Joanne C Mockler, Euan M Wallace, Rebecca Lim
: : Human amnion epithelial cells (hAECs) have been shown to possess potent immunomodulatory properties across a number of disease models. Recently, we reported that hAECs influence macrophage polarization and activity, and that this step was dependent on regulatory T cells. In this study, we aimed to assess the effects of hAEC-derived proresolution lipoxin-A4 (LXA4) on T-cell, macrophage, and neutrophil phenotype and function during the acute phase of bleomycin-induced lung injury. Using C57Bl6 mice, we administered 4 million hAECs intraperitoneally 24 hours after bleomycin challenge...
November 7, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27814615/linking-expression-of-aquaporin-3-to-activation-of-jnk-pathway
#8
Qi Shen, Jingjing Wang, Qingfeng Zhou, Zhaojun Shen, Hui Luo, Xuejiao Tao, Xueqiong Zhu
Aquaporin 3 (AQP3) has been shown to be low in the amnion and chorion tissues of patients with oligohydramnios and that S. miltiorrhiza, a Chinese herbal medicine, results in increased AQP3 in human amniotic epithelial cells (hAECs). Here, we provide evidence for the involvement of the JNK pathway in AQP3 regulation in isolated oligohydramnios tissues in vitro, in hAECs derived from normal amniotic fluid and fluid from patients with isolated oligohydramnios. Phosphorylation of JNK was suppressed by pretreatment of cells with JNK-specific inhibitor (SP600125) and was up-regulated by S...
January 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/27690691/induction-of-amnion-epithelial-apoptosis-by-cortisol-via-tpa-plasmin-system
#9
Wangsheng Wang, Chao Liu, Kang Sun
Rupture of fetal membranes (ROM) can initiate parturition at both term and preterm birth. Apoptosis of the amnion epithelium plays a key role in structural remodeling of the membranes preceding ROM. However, the causative factors for apoptosis remain unidentified. Toward the end of gestation, a feed-forward regeneration of cortisol via 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) exists in the fetal membranes. Here we have examined whether cortisol accumulation is a causative factor for amnion cells apoptosis...
October 3, 2016: Endocrinology
https://www.readbyqxmd.com/read/27647937/serelaxin-improves-the-therapeutic-efficacy-of-rxfp1-expressing-human-amnion-epithelial-cells-in-experimental-allergic-airways-disease
#10
Simon G Royce, Anna M Tominaga, Matthew Shen, Krupesh P Patel, Brooke M Huuskes, Rebecca Lim, Sharon D Ricardo, Chrishan S Samuel
Current asthma therapies primarily target airway inflammation (AI) and suppress episodes of airway hyperresponsiveness (AHR) but fail to treat airway remodelling (AWR), which can develop independently of AI and contribute to irreversible airway obstruction. This study compared the anti-remodelling and therapeutic efficacy of human bone marrow-derived mesenchymal stem cells (MSCs) to that of human amnion epithelial stem cells (AECs) in the setting of chronic allergic airways disease (AAD), in the absence or presence of an anti-fibrotic (serelaxin; RLX)...
September 19, 2016: Clinical Science (1979-)
https://www.readbyqxmd.com/read/27476049/human-amnion-epithelial-cells-expressing-hla-g-as-novel-cell-based-treatment-for-liver-disease
#11
Stephen C Strom, Roberto Gramignoli
Despite routine liver transplantation and supporting medical therapies, thousands of patients currently wait for an organ and there is an unmet need for more refined and widely available regenerative strategies to treat liver diseases. Cell transplants attempt to maximize the potential for repair and/or regeneration in liver and other organs. Over 40years of laboratory pre-clinical research and 25years of clinical procedures have shown that certain liver diseases can be treated by the infusion of isolated cells (hepatocyte transplant)...
September 2016: Human Immunology
https://www.readbyqxmd.com/read/27333275/amnion-epithelial-cell-derived-exosomes-demonstrate-physiologic-state-of-cell-under-oxidative-stress
#12
Samantha Sheller, John Papaconstantinou, Rheanna Urrabaz-Garza, Lauren Richardson, George Saade, Carlos Salomon, Ramkumar Menon
At term, the signals of fetal maturity and feto-placental tissue aging prompt uterine readiness for delivery by transitioning quiescent myometrium to an active stage. It is still unclear how the signals reach the distant myometrium. Exosomes are a specific type of extracellular vesicle (EVs) that transport molecular signals between cells, and are released from a wide range of cells, including the maternal and fetal cells. In this study, we hypothesize that i) exosomes act as carriers of signals in utero-placental compartments and ii) exosomes reflect the physiologic status of the origin cells...
2016: PloS One
https://www.readbyqxmd.com/read/27259996/conditioned-medium-derived-from-rat-amniotic-epithelial-cells-confers-protection-against-inflammation-cancer-and-senescence
#13
Clara Di Germanio, Michel Bernier, Michael Petr, Mauro Mattioli, Barbara Barboni, Rafael de Cabo
Amniotic epithelial cells (AECs) are a class of fetal stem cells that derives from the epiblast and resides in the amnion until birth. AECs are suitable candidates for regenerative medicine because of the ease of collection, their low immunogenicity and inability to form tumors after transplantation. Even though human AECs have been widely investigated, the fact remains that very little is known about AECs isolated from rat, one of the most common animal models in medical testing. In this study, we showed that rat AECs retained stemness properties and plasticity, expressed the pluripotency markers Sox2, Nanog, and Oct4 and were able to differentiate toward the osteogenic lineage...
May 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27217206/inappropriate-cadherin-switching-in-the-mouse-epiblast-compromises-proper-signaling-between-the-epiblast-and-the-extraembryonic-ectoderm-during-gastrulation
#14
M Felicia Basilicata, Marcus Frank, Davor Solter, Thomas Brabletz, Marc P Stemmler
Cadherin switching from E-cadherin (E-cad) to N-cadherin (N-cad) is a key step of the epithelial-mesenchymal transition (EMT) processes that occurs during gastrulation and cancer progression. We investigate whether cadherins actively participate in progression of EMT by crosstalk to signaling pathways. We apply ectopic cadherin switching before the onset of mouse gastrulation. Mutants with an induced E-cad to N-cad switch (Ncadki) die around E8.5. Severe morphological changes including a small epiblast, a rounded shape, an enlarged extra-embryonic compartment and lack of the amnion, combined with a massive cell detachment from the ectodermal layer are detected...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27171794/isolation-of-human-amnion-epithelial-cells-according-to-current-good-manufacturing-procedures
#15
Roberto Gramignoli, Raghuraman C Srinivasan, Kristina Kannisto, Stephen C Strom
Different cell types can be isolated from human placental tissues, and some have been reported to retain phenotypic plasticity and characteristics that make them a promising source of cells for regenerative medicine. Among these are human amnion epithelial cells (hAECs). Adoption of current good manufacturing practices (cGMP) and enhanced quality control is essential when isolating hAECs in order to deliver a safe and effective cellular product for clinical purposes. This unit describes a detailed protocol for selective isolation of hAECs from human term placenta with little to no contamination by other cell types...
2016: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/27149533/different-light-transmittance-of-placental-and-reflected-regions-of-human-amniotic-membrane-that-could-be-crucial-for-corneal-tissue-engineering
#16
Tina Deihim, Ghasem Yazdanpanah, Hassan Niknejad
PURPOSE: Because of long-term incorporation of amniotic membrane (AM) into corneal stroma after transplantation as a scaffold for stem cell delivery, the variation in haziness is a major factor that influences visual quality. The aim of this study was to evaluate probable sources of transparency variation in fresh and freeze-dried AM and compare the obtained results with transparency of rabbit corneas. METHODS: Amnions were extracted from placental and reflected regions of placentas from elective Cesarean sections...
July 2016: Cornea
https://www.readbyqxmd.com/read/27105430/ventilation-induced-brain-injury-in-preterm-neonates-a-review-of-potential-therapies
#17
Samantha K Barton, Mary Tolcos, Suzanne L Miller, Charles Christoph-Roehr, Georg M Schmölzer, Timothy J M Moss, Stuart B Hooper, Euan M Wallace, Graeme R Polglase
Mechanical ventilation is a risk factor for cerebral inflammation and brain injury in preterm neonates. The risk increases proportionally with the intensity of treatment. Recent studies have shown that cerebral inflammation and injury can be initiated in the delivery room. At present, initiation of intermittent positive pressure ventilation (IPPV) in the delivery room is one of the least controlled interventions a preterm infant will likely face. Varying pressures and volumes administered shortly after birth are sufficient to trigger pathways of ventilation-induced lung and brain injury...
2016: Neonatology
https://www.readbyqxmd.com/read/27068127/the-combined-effect-of-pdx1-epidermal-growth-factor-and-poly-l-ornithine-on-human-amnion-epithelial-cells-differentiation
#18
Shruti Balaji, Yu Zhou, Anasuya Ganguly, Emmanuel C Opara, Shay Soker
BACKGROUND: It has been suggested that the ectopic expression of PDX1, a dominant pancreatic transcription factor, plays a critical role in the developmental programming of the pancreas even from cells of unrelated tissues such as keratinocytes and amniotic fluid stem cells. In this study we have chosen to drive pancreatic development in human amnion epithelial cells by inducing endogenous PDX1 expression. Further, we have investigated the role of Epidermal Growth Factor (EGF) and Poly-L-Ornithine (PLO) on this differentiation process...
2016: BMC Developmental Biology
https://www.readbyqxmd.com/read/26917259/aquaporin-3-expression-induced-by-salvia-miltiorrhiza-via-erk1-2-signal-pathway-in-the-primary-human-amnion-epithelium-cells-from-isolated-oligohydramnios
#19
Q Shen, X Ma, Y Hua, M Chen, Y Wang, Q Zhou, W Ye, X Zhu
Salvia miltiorrhiza is one of the most common Chinese herbal drugs, which is effective to treat oligohydramnios. In this study, the aim was to investigate how Salvia miltiorrhiza regulate aquaporin 3 expression in the human amnion epithelial cells (hAECs) with normal amniotic fluid volume or isolated oligohydramnios, whether via extracellular signal regulated kinase1/2 (ERK1/2) signal transduction pathway or not. Primary hAECs cultures from 120 patients were incubated with Salvia miltiorrhiza or/and ERK1/2 inhibitor-- U0126...
2016: Current Molecular Medicine
https://www.readbyqxmd.com/read/26915736/acid-sphingomyelinase-mediates-50-hz-magnetic-field-induced-egf-receptor-clustering-on-lipid-raft
#20
Yong Wang, Xingwen Li, Liyuan Sun, Baihuan Feng, Wenjun Sun
Previously, we found that exposure to a 50-Hz magnetic field (MF) could induce epidermal growth factor receptor (EGFR) clustering and phosphorylation on cell surface. In order to explore the possible mechanisms, the roles of acid sphingomyelinase (ASMase) and lipid raft in MF-induced EGFR clustering were investigated in the present study. Human amnion epithelial (FL) cells were exposed to a 50-Hz MF at 0.4 mT for different durations. Intracellular ASMase activity was detected using the Amplex® Red Sphingomyelinase Assay Kit...
February 26, 2016: Journal of Receptor and Signal Transduction Research
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