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https://www.readbyqxmd.com/read/27918952/evaluation-of-nf-%C3%AE%C2%BAb-concentration-in-patients-with-tick-borne-encephalitis-neuroborreliosis-anaplasmosis-and-anaplasma-phagocythophilum-with-tick-borne-encephalitis-virus-co-infection
#1
Anna Moniuszko-Malinowska, Piotr Czupryna, Justyna Dunaj, Renata Swierzbinska, Katarzyna Guziejko, Ryszard Rutkowski, Joanna Zajkowska, Sambor Grygorczuk, Maciej Kondrusik, Sławomir Pancewicz
OBJECTIVES: The aim of the study was the evaluation of NF-κB concentration in serum and cerebrospinal fluid (CSF) of patients with diagnosis of tick-borne diseases: tick-borne encephalitis (TBE), neuroborreliosis (NB), anaplasmosis (ANA) and patients co-infected with tick-borne encephalitis virus and Anaplasma phagocythophilum (TBE+ANA). Additionally NF-κB concentration during acute and convalescent period was compared. METHODS: Sixty-seven patients with diagnosis of tick-borne diseases were included in the study...
December 2, 2016: Cytokine
https://www.readbyqxmd.com/read/27908984/draft-anaplasma-phagocytophilum-genome-sequences-from-five-cows-two-horses-and-one-roe-deer-collected-in-europe
#2
Thibaud Dugat, Marie-Noëlle Rossignol, Olivier Rué, Valentin Loux, Sylvain Marthey, Marco Moroldo, Cornelia Silaghi, Dirk Höper, Julia Fröhlich, Martin Pfeffer, Erich Zweygarth, Anne-Claire Lagrée, Henri-Jean Boulouis, Nadia Haddad
Anaplasma phagocytophilum is a zoonotic tick-borne intracellular bacterium responsible for granulocytic anaplasmosis. As it is difficult to isolate and cultivate, only 20 A. phagocytophilum genomes have been sequenced to date. Here, we present eight A. phagocytophilum genome sequences obtained using alternative approaches based on sequence capture technology.
December 1, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27898670/gray-wolf-exposure-to-emerging-vector-borne-diseases-in-wisconsin-with-comparison-to-domestic-dogs-and-humans
#3
Rocio F Jara, Adrian P Wydeven, Michael D Samuel
World-wide concern over emerging vector-borne diseases has increased in recent years for both animal and human health. In the United Sates, concern about vector-borne diseases in canines has focused on Lyme disease, anaplasmosis, ehrlichiosis, and heartworm which infect domestic and wild canids. Of these diseases, Lyme and anaplasmosis are also frequently diagnosed in humans. Gray wolves (Canis lupus) recolonized Wisconsin in the 1970s, and we evaluated their temporal and geographic patterns of exposure to these four vector-borne diseases in Wisconsin as the population expanded between 1985 and 2011...
2016: PloS One
https://www.readbyqxmd.com/read/27890399/a-partially-purified-outer-membrane-protein-virb9-1-for-low-cost-nanovaccines-against-anaplasma-marginale
#4
Liang Zhao, Antonino S Cavallaro, David Wibowo, Bing Zhang, Jun Zhang, Neena Mitter, Chengzhong Yu, Chun-Xia Zhao, Anton P J Middelberg
Anaplasma marginale is a devastating tick-borne pathogen causing anaplasmosis in cattle and results in significant economic loss to the cattle industry worldwide. Currently, there is no widely accepted vaccine against A. marginale. New generation subunit vaccines against A. marginale, which are much safer, more efficient and cost-effective, are in great need. The A. marginale outer membrane protein VirB9-1 is a promising antigen for vaccination. We previously have shown that soluble recombinant VirB9-1 protein can be expressed and purified from Escherichia coli and induce a high level of humoral and cellular immunity in mice...
November 24, 2016: Vaccine
https://www.readbyqxmd.com/read/27884441/trypanosoma-vivax-in-water-buffalo-of-the-venezuelan-llanos-an-unusual-outbreak-of-wasting-disease-in-an-endemic-area-of-typically-asymptomatic-infections
#5
Herakles A Garcia, Oneyda J Ramírez, Carla M F Rodrigues, Rafael G Sánchez, Angelica M Bethencourt, Gabriela Del M Pérez, Antonio H H Minervino, Adriana C Rodrigues, Marta M G Teixeira
Trypanosoma vivax has been associated with asymptomatic infections in African and South American buffalo. In this study, T. vivax was analyzed in water buffalo (Bubalus bubalis) from Venezuela in a molecular survey involving 293 blood samples collected from 2006 to 2015 across the Llanos region. Results demonstrated constant infections (average 23%) during the years analyzed. In general, animals were healthy carriers of T. vivax with low levels of parasitemia and were diagnosed exclusively by TviCATL-PCR. However, an outbreak of severe acute infections mostly in dairy animals was reported during a prolonged drought affecting 30...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27883378/wild-animals-as-reservoirs-of-anaplasma-phagocytophilum-for-humans
#6
Beata Dzięgiel, Łukasz Adaszek, Stanisław Winiarczyk
Anaplasma phagocytophilum is a tick-transmitted obligate-intracellular gram-negative bacteria that causes emerging human zoonosis. A. phagocytophilum is transmitted by Ixodid ticks. Recent studies suggest that wild animals may be reservoirs of A. phagocytophilum for humans. The organism infects and survives within neutrophils. The infection diagnosis is based on the detection of morulae within granulocytes of peripheral blood, results of serological tests and detection of the DNA of A. phagocytophilum using specific polymerase chain reaction assays (PCR)...
2016: Przegla̧d Epidemiologiczny
https://www.readbyqxmd.com/read/27876614/first-molecular-detection-and-genetic-analysis-of-anaplasma-phagocytophilum-in-shelter-cats-in-seoul-korea
#7
Seung-Hun Lee, Dorene VanBik, Neung-Hee Kim, Sang-Joon Park, Oh-Deog Kwon, Tae-Hwan Kim, Dongmi Kwak
Here, we report the molecular detection of Anaplasma phagocytophilum in shelter cats in Korea and the relationships between A. phagocytophilum gene sequences and the pathogenicity, region, and host specificity of this bacterium. Two (0.9%) out of 222 shelter cats from Seoul, Korea, yielded positive results for the A. phagocytophilum 16S rRNA, groEL, and msp2 genes. Phylogenetic analysis divided groEL gene sequences into two groups (alanine and serine), based on their nucleotide and amino acid sequences. A. phagocytophilum msp2 gene sequences were grouped per the region of isolation (Europe vs...
December 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27860010/detection-of-lyme-disease-and-anaplasmosis-pathogens-via-pcr-in-pennsylvania-deer-ked
#8
M Buss, L Case, B Kearney, C Coleman, J D Henning
Borrelia burgdorferi and Anaplasma phagocytophilum are obligate intracellular parasites that maintain their life cycles in enzoonotic vector-host cycles with Ixodes scapularis as a vector. In addition to ticks, the hosts are commonly infested with insects from the Hippoboscidae family. This study confirms the presence of B. burgdorferi and A. phagocytophilum in deer keds (Lipoptena cervi) removed from white-tailed deer using PCR. Detection of these pathogens in deer ked represents a potential novel susceptibility of wildlife and also suggests the risk of transmission of these pathogens to humans and animals alike through the bite of an infected ectoparasite...
December 2016: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/27812829/anaplasma-phagocytophilum-in-questing-ixodes-ricinus-ticks-in-southwestern-finland
#9
Jani J Sormunen, Ritva Penttinen, Tero Klemola, Eero J Vesterinen, Jari Hänninen
Anaplasma phagocytophilum is the causative agent of an emerging tick-borne disease, human granulocytic anaplasmosis. While the bacterium has been reported from questing ticks in neighboring Sweden, Norway and Russia, the few surveys regarding questing ticks in Finland have thus far been negative. In the current study, the prevalence of A. phagocytophilum in Ixodes ricinus populations was evaluated in several study localities around southwestern Finland during 2013-2014. Some of these populations were previously screened and found negative for A...
November 3, 2016: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/27787614/molecular-diagnosis-of-the-tick-borne-pathogen-anaplasma-marginale-in-cattle-blood-samples-from-nigeria-using-qpcr
#10
Nusirat Elelu, Joana Ferrolho, Joana Couto, Ana Domingos, Mark C Eisler
Tick-borne diseases (TBDs) are some of the most important animal health and management problems in Africa, including Nigeria. This study aims to determine the prevalence of an important TBD, anaplasmosis, in a North-central region of Nigeria. Blood samples were collected from cattle and stored on Whatman FTA(®) cards. Information on village, age and sex associated with each cattle was also recorded. The packed red blood cell volume (PCV) for each blood sample was determined. After DNA extraction, pathogen presence was evaluated by TaqMan(®) based qPCR of which 75...
October 27, 2016: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/27785765/long-term-effects-of-good-handling-practices-during-the-pre-weaning-period-of-crossbred-dairy-heifer-calves
#11
Luciana Pontes Silva, Aline Cristina Sant'Anna, Lívia Carolina Magalhães Silva, Mateus José Rodrigues Paranhos da Costa
The aim of this study was to determine whether applying good practices of handling during the pre-weaning period have long-term effects on behavioral and physiological indicators, health status, and average daily gain (ADG) of crossbred Bos taurus × Bos indicus heifer calves. During the pre-weaning period, 98 crossbred of Holstein × Gir heifer calves were allotted into three treatments: (1) good practices of handling + brushing (GPB; n = 25), (2) good practices of handling (GP; n = 25), and (3) control (n = 48)...
October 26, 2016: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27759758/enzootic-instability-for-bovine-anaplasmosis-on-family-farms-located-in-southwestern-paran%C3%A3-brazil
#12
Tatiane Sott, Carina Franciscato, Adolfo Firmino da Silva, Iucif Abrão Nascif, Fagner Luiz da Costa Freitas
The objective of this study was to assess the occurence of animals seropositive for Anaplasma marginale in the municipality of Realeza, Paraná State, Brazil. Blood samples were collected from 344 cows on 18 small farms in the municipality of Realeza-PR. The animals'serum samples were forwarded to the Federal University of Fronteira do Sul, in order to investigate the occurrence of anti-A. marginale IgG antibodies by an enzyme-linked immunosorbent assay commercial kit. IgG antibodies to A. marginale were detected in cattle from 77...
October 13, 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/27725355/immune-exhaustion-during-chronic-infections-in-cattle
#13
Satoru Konnai, Shiro Murata, Kazuhiko Ohashi
Recently, dysfunction of antigen-specific T cells is well documented as T-cell exhaustion and has been defined by the loss of effector functions during chronic infections and cancer in human. The exhausted T cells are characterized phenotypically by the surface expression of immunoinhibitory receptors, such as programmed death 1 (PD-1), lymphocyte activation gene 3 (LAG-3), T-cell immunoglobulin and mucin domain-containing protein 3 (Tim-3) and cytotoxic T-lymphocyte antigen 4 (CTLA-4). However, there is still a fundamental lack of knowledge about the immunoinhibitory receptors in the fields of veterinary medicine...
October 8, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27713867/anaplasma-phagocytophilum-aph0032-is-exposed-on-the-cytosolic-face-of-the-pathogen-occupied-vacuole-and-co-opts-host-cell-sumoylation
#14
Aminat T Oki, Bernice Huang, Andrea R Beyer, Levi J May, Hilary K Truchan, Naomi J Walker, Nathan L Galloway, Dori L Borjesson, Jason A Carlyon
Anaplasma phagocytophilum, a member of the family Anaplasmataceae and the obligate intracellular bacterium that causes granulocytic anaplasmosis, resides in a host cell-derived vacuole. Bacterial proteins that localize to the A. phagocytophilum-occupied vacuole membrane (AVM) are critical host-pathogen interfaces. Of the few bacterial AVM proteins that have been identified, the domains responsible for AVM localization and the host cell pathways that they co-opt are poorly defined. APH0032 is an effector that is expressed and localizes to the AVM late during the infection cycle...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27666778/natural-anaplasmataceae-infection-in-rhipicephalus-bursa-ticks-collected-from-sheep-in-the-french-basque-country
#15
Mustapha Dahmani, Bernard Davoust, Frédéric Rousseau, Didier Raoult, Florence Fenollar, Oleg Mediannikov
Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27648639/accuracy-of-diagnosis-of-human-granulocytic-anaplasmosis-in-china
#16
Gary P Wormser
In 2008, human granulocytic anaplasmosis (HGA) was reported from China. However, the clinical and laboratory findings, including reports of nosocomial transmission, were inconsistent with those reported for HGA in the United States. In 2012, it was demonstrated that the patients described in the 2008 report had all been infected with a newly discovered bunyavirus, severe fever with thrombocytopenia syndrome virus, which causes an illness with the same clinical features described for the patients in the 2008 report...
October 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/27610385/anaplasma-marginale-diversity-virulence-and-vaccine-landscape-through-a-genomics-approach
#17
REVIEW
Rosa Estela Quiroz-Castañeda, Itzel Amaro-Estrada, Sergio Darío Rodríguez-Camarillo
In order to understand the genetic diversity of A. marginale, several efforts have been made around the world. This rickettsia affects a significant number of ruminants, causing bovine anaplasmosis, so the interest in its virulence and how it is transmitted have drawn interest not only from a molecular point of view but also, recently, some genomics research have been performed to elucidate genes and proteins with potential as antigens. Unfortunately, so far, we still do not have a recombinant anaplasmosis vaccine...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27592064/prevalence-and-genetic-characterization-of-anaplasma-marginale-in-zebu-cattle-bos-indicus-and-their-ticks-amblyomma-variegatum-rhipicephalus-microplus-from-madagascar
#18
Daniela Pothmann, Sven Poppert, Raphael Rakotozandrindrainy, Benedikt Hogan, Mariano Mastropaolo, Claudia Thiel, Cornelia Silaghi
Tick-borne bovine anaplasmosis, caused by the obligate intracellular pathogen Anaplasma marginale (Rickettsiales: Anaplasmataceae), is a major constraint to cattle production in tropical and subtropical regions. From Madagascar, clinical cases were published but data based on molecular methods regarding the prevalence and genetic diversity of this pathogen on the island are lacking. The aims of this study were to investigate (1) the prevalence of A. marginale in Malagasy zebu cattle (Bos indicus) and their ticks with a species-specific real-time PCR, (2) the genetic diversity of A...
October 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27585218/the-eye-and-tick-borne-disease-in-the-united-states
#19
Saraniya Sathiamoorthi, Wendy M Smith
PURPOSE OF REVIEW: Tick-borne diseases are increasing in incidence and geographic distribution. Several diseases endemic to the United States have ophthalmic manifestations, including the most common tick-borne disease, Lyme borreliosis. As ocular complaints may lead a patient to seek medical evaluation, it is important to be aware of the systemic and ophthalmic manifestations of tick-borne diseases in order to make the correct diagnosis. RECENT FINDINGS: Vision-threatening ophthalmic manifestations are relatively common in Lyme disease and Rocky Mountain spotted fever...
November 2016: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/27571570/canine-bicavitary-carcinomatosis-with-transient-needle-tract-metastasis-diagnosed-by-multiplex-immunocytochemistry
#20
A Russell Moore, Emily Coffey, Sarah E Leavell, Greta Krafsur, Colleen Duncan, Kristy Dowers, Kelly S Santangelo
A 6-year-old, male castrated, mixed-breed dog was referred to the James L. Voss Veterinary Teaching Hospital at Colorado State University for bicavitary effusion. On examination, the dog was tachycardic and tachypneic with bilaterally decreased lung sounds. Thoracic and abdominal ultrasonic examination revealed pleural and peritoneal effusions, which were aspirated and submitted for fluid analysis and cytology. Both cavity fluids were classified as exudates with a large population of vacuolated mononuclear cells...
September 2016: Veterinary Clinical Pathology
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