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NDRG1

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https://www.readbyqxmd.com/read/28325852/extended-multiplexing-of-tmt-labeling-reveals-age-and-high-fat-diet-specific-proteome-changes-in-mouse-epididymal-adipose-tissue
#1
Deanna L Plubell, Phillip A Wilmarth, Yuqi Zhao, Alexandra M Fenton, Jessica Minnier, Ashok P Reddy, John Klimek, Xia Yang, Larry L David, Nathalie Pamir
The lack of high-throughput methods to analyze the adipose tissue protein composition limits our understanding of the protein networks responsible for age and diet related metabolic response. We have developed an approach using multiple-dimension liquid chromatography tandem mass spectrometry and extended multiplexing (24 biological samples) with TMT labeling to analyze proteomes of epididymal adipose tissues isolated from mice fed either low or high fat diet for a short or a long-term, and from mice that aged on low vs...
March 21, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28292472/increased-ndrg1-expression-attenuate-trophoblast-invasion-through-erk-mmp-9-pathway-in-preeclampsia
#2
Yufen Fu, Jufeng Wei, Xueli Dai, Yuanhua Ye
OBJECTIVE: The aim of this study was to investigate the expression of N-myc downstream-regulated gene1(NDRG1)in the placentas of pregnancies complicated with early-onset and late-onset preeclampsia (PE) and its underlying mechanism on the pathophysiology of PE. METHODS: The expressions of NDRG-1 in placentas of pregnancies complicated with early-onset PE and late-onset PE were detected using immunohistochemistry, western blot assays and fluorescence quantitative PCR...
March 2017: Placenta
https://www.readbyqxmd.com/read/28275050/novel-thiosemicarbazones-inhibit-lysine-rich-ceacam1-co-isolated-lyric-and-the-lyric-induced-epithelial-mesenchymal-transition-via-up-regulation-of-n-myc-downstream-regulated-gene-1-ndrg1
#3
Ruxing Xi, Ivan Ho Yuen Pun, Sharleen V Menezes, Leyla Fouani, Danuta S Kalinowski, Michael L H Huang, Xiaozhi Zhang, Des R Richardson, Zaklina Kovacevic
Tumor necrosis factor α (TNFα) plays a vital role in cancer progression, being associated with inflammation and promotion of cancer angiogenesis and metastasis. The effects of TNFα are mediated by its down-stream target, the oncogene, lysine-rich CEACAM1 co-isolated protein (LYRIC; also known as metadherin or astrocyte elevated gene-1). LYRIC plays an important role in activating the nuclear factor-κB (NF-κB) signaling pathway, which controls multiple cellular processes, including proliferation, apoptosis, migration, etc...
March 8, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28222113/differentially-expressed-proteins-underlying-childhood-cortical-dysplasia-with-epilepsy-identified-by-itraq-proteomic-profiling
#4
Lu Qin, Xi Liu, Shiyong Liu, Yi Liu, Yixuan Yang, Hui Yang, Yangmei Chen, Lifen Chen
Cortical dysplasia accounts for at least 14% of epilepsy cases, and is mostly seen in children. However, the understanding of molecular mechanisms and pathogenesis underlying cortical dysplasia is limited. The aim of this cross-sectional study is to identify potential key molecules in the mechanisms of cortical dysplasia by screening the proteins expressed in brain tissues of childhood cortical dysplasia patients with epilepsy using isobaric tags for relative and absolute quantitation-based tandem mass spectrometry compared to controls, and several differentially expressed proteins that are not reported to be associated with cortical dysplasia previously were selected for validation using real-time polymerase chain reaction, immunoblotting and immunohistochemistry...
2017: PloS One
https://www.readbyqxmd.com/read/28191699/ndrg1-is-important-to-maintain-the-integrity-of-airway-epithelial-barrier-through-claudin-9-expression
#5
Yasuhiro Gon, Shuichiro Maruoka, Hiroyuki Kishi, Yutaka Kozu, Kuroda Kazumichi, Yasuyuki Nomura, Ikuko Takeshita, Takeshi Oshima, Shu Hashimoto
Impairment of epithelial barrier integrity caused by environmental triggers is associated with the pathogenesis of airway inflammation. Using human airway epithelial cells, we attempted to identify molecule(s) that promote airway epithelial barrier integrity. Microarray analyses were conducted using the Affimetrix human whole genome gene chip, and we identified the N-myc downstream-regulated gene 1 (NDRG1) gene, which was induced during the development of the epithelial cell barrier. Immunohistochemical analysis revealed strong NDRG1 expression in ciliated epithelial cells in nasal tissues sampled from patients with chronic rhinosinusitis (CRS), and the low expression of NDRG1 was observed in goblet cells or damaged epithelial cells...
February 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28181403/caffeic-acid-phenethyl-ester-upregulates-n-myc-downstream-regulated-gene-1-via-erk-pathway-to-inhibit-human-oral-cancer-cell-growth-in-vitro-and-in-vivo
#6
Li-Chuan Chung, Kun-Chun Chiang, Tsui-Hsia Feng, Kang-Shuo Chang, Sung-Ting Chuang, Yu-Jen Chen, Ke-Hung Tsui, Jehn-Chuan Lee, Horng-Heng Juang
SCOPE: Caffeic acid phenethyl ester (CAPE), a bioactive component of propolis, is considered as a new anti-cancer agent. Oral squamous cell carcinoma (OSCC) is the most common oral cancer with unsatisfying survival. N-myc downstream regulated family genes (NDRGs) involve in numerous physiological processes. We investigated the anti-cancer effect of CAPE on OSCC and related mechanisms. METHODS AND RESULTS: Cell proliferation assay, western blot, gene transfection and knockdown, and reporter assay were applied...
February 9, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28075464/downregulation-of-the-n-myc-downstream-regulated-gene-1-is-related-to-enhanced-proliferation-invasion-and-migration-of-pancreatic-cancer
#7
Gang Cen, Kundong Zhang, Jun Cao, Zhengjun Qiu
The N-myc downstream regulated gene 1 (NDRG1) is differently expressed in human malignancies according to the tumor type. We investigated the expression of NDRG1 in pancreatic cancer tissues and cell lines as well as how it affects tumor growth, invasion and migration in pancreatic cancer cells. Experimental groups included NDRG1 overexpression and knockdown pancreatic cancer cell lines. Lentivirus-based empty vector transfected cells (NC group) were considered control groups. Proliferation, invasion and migration related proteins such as STAT3, MMPs, PTEN, PI3K/AKT were assessed by CCK-8, Transwell assay and western blotting...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/28058793/effects-of-selection-for-ethanol-preference-on-gene-expression-in-the-nucleus-accumbens-of-hs-cc-mice
#8
A M Colville, O D Iancu, D L Oberbeck, P Darakjian, C L Zheng, N A R Walter, C A Harrington, R P Searles, S McWeeney, R J Hitzemann
Previous studies on changes in murine brain gene expression associated with the selection for ethanol preference have used F2 intercross or heterogeneous stock (HS) founders, derived from standard laboratory strains. However, these populations represent only a small proportion of the genetic variance available in Mus musculus. To investigate a wider range of genetic diversity, we selected mice for ethanol preference using an HS derived from the eight strains of the collaborative cross. These HS mice were selectively bred (four generations) for high and low ethanol preference...
January 6, 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28004151/reciprocal-regulation-of-mtor-complexes-in-pancreatic-islets-from-humans-with-type-2-diabetes
#9
Ting Yuan, Sahar Rafizadeh, Kanaka Durga Devi Gorrepati, Blaz Lupse, Jose Oberholzer, Kathrin Maedler, Amin Ardestani
AIMS/HYPOTHESIS: Mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of nutritional status at the cellular and organismic level. While mTORC1 mediates beta cell growth and expansion, its hyperactivation has been observed in pancreatic islets from animal models of type 2 diabetes and leads to beta cell loss. We sought to determine whether such mTORC1 activation occurs in humans with type 2 diabetes or in metabolically stressed human islets and whether mTORC1 blockade can restore beta cell function of diabetic islets...
December 21, 2016: Diabetologia
https://www.readbyqxmd.com/read/27982524/a-novel-ndrg1-mutation-in-a-non-romani-patient-with-cmt4d-hmsn-lom
#10
Giuseppe Piscosquito, Stefania Magri, Paola Saveri, Micaela Milani, Claudia Ciano, Laura Farina, Franco Taroni, Davide Pareyson
Charcot-Marie-Tooth disease type 4D (CMT4D), also known as hereditary motor and sensory neuropathy Lom type (HMSNL), is an autosomal recessive, early onset, severe demyelinating neuropathy with hearing loss, caused by N-Myc downstream-regulated gene 1 (NDRG1) mutations. CMT4D is rare with only three known mutations, one of which (p.Arg148Ter) is found in patients of Romani ancestry and accounts for the vast majority of cases. We report a 38-year-old Italian female with motor development delay, progressive neuropathy, and sensorineural deafness...
March 2017: Journal of the Peripheral Nervous System: JPNS
https://www.readbyqxmd.com/read/27955744/glycoproteomics-analysis-of-plasma-proteins-associated-with-opisthorchis-viverrini-infection-induced-cholangiocarcinoma-in-hamster-model
#11
Pramote Sriwanitchrak, Atchara Paemanee, Sittiruk Roytrakul, Vithoon Viyanant, Kesara Na-Bangchang
OBJECTIVE: To apply lectin affinity chromatography and glycoproteomics-based LC-MS/MS to preliminarily investigate the possible potential plasma biomarkers of Opisthorchis viverrini (OV)-associated CCA in OV/dimethylnitrosamine (DMN)-induced CCA hamster model. METHODS: Nine Syrian hamsters were divided into 3 groups as follows (n = 3 each): normal (healthy control group); OV group; and OV/DMN group (CCA group). Pooled plasma samples collected from animals in each group at the 6th month post-infection with OV metacercarae were subjected to glycoproteomics analysis...
December 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27894074/lsd1-mediates-mycn-control-of-epithelial-mesenchymal-transition-through-silencing-of-metastatic-suppressor-ndrg1-gene
#12
Susanna Ambrosio, Stefano Amente, Carmen D Saccà, Mario Capasso, Raffaele A Calogero, Luigi Lania, Barbara Majello
Neuroblastoma (NB) with MYCN amplification is a highly aggressive and metastatic tumor in children. The high recurrence rate and resistance of NB cells to drugs urgently demands a better therapy for this disease. We have recently found that MYCN interacts with the lysine-specific demethylase 1 (LSD1), a histone modifier that participates in key aspects of gene transcription. In cancer cells, LSD1 contributes to the genetic reprogramming that underlies to Epithelial-Mesenchymal Transition (EMT) and tumor metastasis...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27831650/critical-genes-of-hepatocellular-carcinoma-revealed-by-network-and-module-analysis-of-rna-seq-data
#13
M-R Yang, Y Zhang, X-X Wu, W Chen
OBJECTIVE: RNA-seq data of hepatocellular carcinoma (HCC) was analyzed to identify critical genes related to the pathogenesis and prognosis. MATERIALS AND METHODS: Three RNA-seq datasets of HCC (GSE69164, GSE63863 and GSE55758) were downloaded from Gene Expression Omnibus (GEO), while another dataset including 54 HCC cases with survival time was obtained from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) were identified by significant analysis of microarrays (SAM) method using package samr of R...
October 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27830690/-detection-of-novel-genetic-markers-of-susceptibility-to-preeclampsia-based-on-an-analysis-of-the-regulatory-genes-in-the-placental-tissue
#14
V N Serebrova, E A Trifonova, T V Gabidulina, I Yu Bukharina, T A Agarkova, I D Evtushenko, N R Maksimova, V A Stepanov
Regulatory single nucleotide polymorphisms (rSNPs) are the least-studied group of SNP; however, they play an essential role in the development of human pathology by altering the level of candidate genes expression. In this work, we analyzed 29 rSNPs in 17 new candidate genes associated with preeclampsia (PE) according to the analysis of the transcriptome in placental tissue. Three ethnic groups have been studied (yakut, russian, and buryat). We have detected significant associations of PE with eight rSNPs in six differentially expressed genes, i...
September 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/27811999/effects-of-ndrg1-family-proteins-on-photoreceptor-outer-segment-morphology-in-zebrafish
#15
Shimpei Takita, Yasutaka Wada, Satoru Kawamura
Rods and cones are functionally and morphologically distinct. We previously identified N-myc downstream-regulated gene 1b (ndrg1b) in carp as a cone-specific gene. Here, we show that NDRG1b and its paralog, NDRG1a-1, contribute to photoreceptor outer segment (OS) formation in zebrafish. In adult zebrafish photoreceptors, NDRG1a-1 was localized in the entire cone plasma membranes, and also in rod plasma membranes except its OS. NDRG1b was expressed specifically in cones in the entire plasma membranes. In a developing retina, NDRG1a-1 was expressed in the photoreceptor layer, and NDRG1b in the photoreceptor layer plus inner nuclear layer...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27793840/targeting-of-pyk2-synergizes-with-egfr-antagonists-in-basal-like-tnbc-and-circumvents-her3-associated-resistance-via-the-nedd4-ndrg1-axis
#16
Nandini Verma, Anna-Katharina Müller, Charu Kothari, Effrosini Panayotopoulou, Amir Kedan, Michael Selitrennik, Gordon B Mills, Lan K Nguyen, Sungyoung Shin, Thomas Karn, Uwe Holtrich, Sima Lev
Triple-negative breast cancer (TNBC) is a highly aggressive, heterogeneous disease with poor prognosis and no effective targeted therapies. EGFR is highly expressed in basal-like TNBC and is considered as a potential therapeutic target. However, EGFR targeting exerts only marginal clinical benefits, possibly due to activation of compensatory signaling pathways, which are frequently associated with HER3 upregulation. Here we show that concomitant targeting of EGFR and the nonreceptor tyrosine kinases PYK2/FAK synergistically inhibits the proliferation of basal-like TNBC cells in vitro and attenuates tumor growth in a mouse xenograft model...
January 1, 2017: Cancer Research
https://www.readbyqxmd.com/read/27782193/lipocalin-2-lcn2-is-a-promising-target-for-cholangiocarcinoma-treatment-and-bile-lcn2-level-is-a-potential-cholangiocarcinoma-diagnostic-marker
#17
Kun-Chun Chiang, Ta-Sen Yeh, Ren-Chin Wu, Jong-Hwei S Pang, Chi-Tung Cheng, Shang-Yu Wang, Horng-Heng Juang, Chun-Nan Yeh
Cholangiocarcinoma (CCA) is a devastating disease due to resistance to traditional chemotherapies and radiotherapies. New therapeutic strategies against CCA are urgently needed. This study investigated the role of lipocalin-2 (LCN2) in human cholangiocarcinoma as a potential therapeutic target and diagnostic marker. So far, the role of LCN2 in cancer is still controversial and studies regarding the role of LCN2 in CCA are limited. LCN2 knockdown inhibited CCA cell growth in vitro and in vivo through induction of cell cycle arrest at G0/G1 phases and decreased metastatic potential due to repression of epithelial-mesenchymal transition (EMT)...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27716814/low-levels-of-ndrg1-in-nerve-tissue-are-predictive-of-severe-paclitaxel-induced-neuropathy
#18
Raghav Sundar, Anand D Jeyasekharan, Brendan Pang, Richie Chuan Teck Soong, Nesaretnam Barr Kumarakulasinghe, Samuel Guan Wei Ow, Jingshan Ho, Joline Si Jing Lim, David Shao Peng Tan, Einar P V Wilder-Smith, Aishwarya Bandla, Stacey Sze Hui Tan, Bernadette Reyna Asuncion, Zul Fazreen, Michal Marek Hoppe, Thomas Choudary Putti, Lay Mui Poh, Boon Cher Goh, Soo-Chin Lee
INTRODUCTION: Sensory peripheral neuropathy caused by paclitaxel is a common and dose limiting toxicity, for which there are currently no validated predictive biomarkers. We investigated the relationship between the Charcot-Marie-Tooth protein NDRG1 and paclitaxel-induced neuropathy. METHODS/MATERIALS: Archived mammary tissue specimen blocks of breast cancer patients who received weekly paclitaxel in a single centre were retrieved and NDRG1 immunohistochemistry was performed on normal nerve tissue found within the sample...
2016: PloS One
https://www.readbyqxmd.com/read/27711123/language-agnostic-reproducible-data-analysis-using-literate-programming
#19
Boris Vassilev, Riku Louhimo, Elina Ikonen, Sampsa Hautaniemi
A modern biomedical research project can easily contain hundreds of analysis steps and lack of reproducibility of the analyses has been recognized as a severe issue. While thorough documentation enables reproducibility, the number of analysis programs used can be so large that in reality reproducibility cannot be easily achieved. Literate programming is an approach to present computer programs to human readers. The code is rearranged to follow the logic of the program, and to explain that logic in a natural language...
2016: PloS One
https://www.readbyqxmd.com/read/27698340/golgi-phosphoprotein-3-inhibits-the-apoptosis-of-human-glioma-cells-in-part-by-downregulating-n-myc-downstream-regulated-gene-1
#20
Xin Li, Mengyou Li, Xiuli Tian, Qingzhe Li, Qingyang Lu, Qingbin Jia, Lianqun Zhang, Jinqiang Yan, Xueyuan Li, Xingang Li
BACKGROUND Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in the development of several human cancers. Our previous study showed that GOLPH3 expression in glioma tissues was related to the severity of the malignancy of the cancer. However, the mechanism by which GOLPH3 affects cell apoptosis is largely unknown. The present study was designed to explore the possible mechanism of GOLPH3 in cell apoptosis. MATERIAL AND METHODS To analyze the biological role of GOLPH3 in glioma cells, we used GOLPH3 small interference RNA in apoptosis of glioma cells...
October 4, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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