keyword
MENU ▼
Read by QxMD icon Read
search

Conditional knock-out

keyword
https://www.readbyqxmd.com/read/28437187/synergy-between-choroid-plexus-epithelial-cells-conditioned-medium-and-knockout-serum-replacement-converts-human-adipose-derived-stem-cells-to-dopamine-secreting-neurons
#1
Mahdi Eskandarian Boroujeni, Mossa Gardaneh, Mehrnoosh Hasan Shahriari, Abbas Aliaghaei, Sanaz Hasani
Human adipose-derived stem cells (hADSCs) have great capacity to differentiate into mesodermal origins as well as non-mesodermal lineages including neural cells. This valuable feature paves the way for the therapeutic application of hADSCs for neurodegenerative maladies such as Parkinson's disease (PD). We tested the capacity of Choroid Plexus epithelial cells-conditioned medium (CPEC-CM) alone or cocktailed with knock-out serum (KS) to induce dopaminergic differentiation of hADSCs. To this end, hADSCs from lipoaspirate were phenotypically characterized and shown to maintain mesodermal multipotency so selected media easily differentiated them into osteoblasts, chondrocytes and adipocytes...
March 2, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28432097/diazotrophy-overcomes-the-deleterious-growth-phenotype-of-glne-deletion-in-azotobacter-vinelandii
#2
Florence Mus, Alex Tseng, Ray Dixon, John W Peters
Overcoming the inhibitory effects of excess environmental ammonium on nitrogenase synthesis or activity, or preventing ammonium assimilation, have both been considered as strategies to increase the amount of fixed nitrogen transferred from bacterial to plant partners in associative or symbiotic plant-diazotroph relationships. The GlnE adenylyltransferase/adenylyl-removing enzyme catalyzes reversible adenylylation of glutamine synthetase (GS), thereby affecting the post translational regulation of ammonium assimilation critical for appropriate coordination of carbon and nitrogen assimilation...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28429761/a-specific-nanobody-prevents-amyloidogenesis-of-d76n-%C3%AE-2-microglobulin-in-vitro-and-modifies-its-tissue-distribution-in-vivo
#3
Sara Raimondi, Riccardo Porcari, P Patrizia Mangione, Guglielmo Verona, Julien Marcoux, Sofia Giorgetti, Graham W Taylor, Stephan Ellmerich, Maurizio Ballico, Stefano Zanini, Els Pardon, Raya Al-Shawi, J Paul Simons, Alessandra Corazza, Federico Fogolari, Manuela Leri, Massimo Stefani, Monica Bucciantini, Julian D Gillmore, Philip N Hawkins, Maurizia Valli, Monica Stoppini, Carol V Robinson, Jan Steyaert, Gennaro Esposito, Vittorio Bellotti
Systemic amyloidosis is caused by misfolding and aggregation of globular proteins in vivo for which effective treatments are urgently needed. Inhibition of protein self-aggregation represents an attractive therapeutic strategy. Studies on the amyloidogenic variant of β2-microglobulin, D76N, causing hereditary systemic amyloidosis, have become particularly relevant since fibrils are formed in vitro in physiologically relevant conditions. Here we compare the potency of two previously described inhibitors of wild type β2-microglobulin fibrillogenesis, doxycycline and single domain antibodies (nanobodies)...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28417080/applying-a-systems-approach-to-thyroid-physiology-looking-at-the-whole-with-a-mitochondrial-perspective-instead-of-judging-single-tsh-values-or-why-we-should-know-more-about-mitochondria-to-understand-metabolism
#4
REVIEW
Roy Moncayo, Helga Moncayo
Classical thinking in endocrine physiology squeezes our diagnostic handling into a simple negative feedback mechanism with a controller and a controlled variable. In the case of the thyroid this is reduced to TSH and fT3 and fT4, respectively. The setting of this tight notion has no free space for any additions. In this paper we want to challenge this model of limited application by proposing a construct based on a systems approach departing from two basic considerations. In first place since the majority of cases of thyroid disease develop and appear during life it has to be considered as an acquired condition...
June 2017: BBA Clinical
https://www.readbyqxmd.com/read/28410129/epigenetics-of-reproductive-infertility
#5
Laxmidhar Das, Sabnam Parbin, Nibedita Pradhan, Chahat Kausar, Samir K Patra
Infertility is a complex pathophysiological condition. It may caused by specific or multiple physical and physiological factors, including abnormalities in homeostasis, hormonal imbalances and genetic alterations. In recent times various studies implicated that, aberrant epigenetic mechanisms are associated with reproductive infertility. There might be transgenerational effects associated with epigenetic modifications of gametes and studies suggest the importance of alterations in epigenetic modification at early and late stages of gametogenesis...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28409342/zebrafish-as-a-model-of-kidney-disease
#6
Elvin E Morales, Rebecca A Wingert
Animal models have been an invaluable means to advance biomedical research as they provide experimental avenues for cellular and molecular investigations of disease pathology. The zebrafish (Danio rerio) is a good alternative to mammalian models that can be used to apply powerful genetic experimental methods normally used in invertebrates to answer questions about vertebrate development and disease. In the case of the kidney, the zebrafish has proven itself to be an applicable and versatile experimental system, mainly due to the simplicity of its pronephros, which contains two nephrons that possess conserved structural and physiological aspects with mammalian nephrons...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/28401532/gram-positive-pneumonia-augments-non-small-cell-lung-cancer-metastasis-via-host-toll-like-receptor-2-activation
#7
Stephen D Gowing, Simon C Chow, Jonathan J Cools-Lartigue, Crystal B Chen, Sara Najmeh, Henry Y Jiang, France Bourdeau, Annie Beauchamp, Ugo Mancini, Isabelle Angers, Betty Giannias, Jonathan D Spicer, Simon Rousseau, Salman T Qureshi, Lorenzo E Ferri
Surgical resection of early stage non-small cell lung cancer (NSCLC) is necessary for cure. However, rates of post-operative bacterial pneumonias remain high and may confer an increased risk for metastasis. Toll-like receptors (TLRs) mediate the inflammatory cascade by recognizing microbial products at the surface of numerous cell types in the lung; however, little is known about how host TLRs influence NSCLC metastasis. TLR2 recognizes gram-positive bacterial cell wall components activating innate immunity...
April 12, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28397054/effects-of-rna-interference-combined-with-ultrasonic-irradiation-and-sonovue-microbubbles-on-expression-of-stat3-gene-in-keratinocytes-of-psoriatic-lesions
#8
Li-Wei Ran, Hao Wang, Dong Lan, Hong-Xia Jia, Si-Si Yu
The most effective sequence of small interfering RNA (siRNA) silencing STAT3 of psoriatic keratinocytes (KCs) was screened out, and the effects of the most effective siRNA combined with ultrasonic irradiation and SonoVue microbubbles on the expression of STAT3 of KCs and the dose- and time-response were investigated. Three chemically-synthetic siRNAs targeting STAT3 carried by Lipofectamine 3000 were transfected into KCs, and the effects on STAT3 expression were detected, then the most effective siRNA was selected for the subsequent experiments...
April 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28392759/calcium-dependent-and-synapsin-dependent-pathways-for-the-presynaptic-actions-of-bdnf
#9
Qing Cheng, Sang-Ho Song, George J Augustine
We used cultured hippocampal neurons to determine the signaling pathways mediating brain-derived neurotrophic factor (BDNF) regulation of spontaneous glutamate and GABA release. BDNF treatment elevated calcium concentration in presynaptic terminals; this calcium signal reached a peak within 1 min and declined in the sustained presence of BDNF. This BDNF-induced transient rise in presynaptic calcium was reduced by SKF96365, indicating that BDNF causes presynaptic calcium influx via TRPC channels. BDNF treatment increased the frequency of miniature excitatory postsynaptic currents (mEPSCs)...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28390857/targeted-deletion-of-the-hoxa-cluster-affects-b-lymphopoiesis-through-depletion-of-lymphoid-progenitors
#10
Charles-Étienne Lebert-Ghali, Alexander Thompson, Heather J Melichar, Janet J Bijl
It is well established that Hoxa genes play a critical role in the proliferative capacity of adult hematopoietic stem and progenitor cells, but the importance of Hoxa genes in later stages of hematopoietic differentiation is less clear. Previously, we observed that B cell numbers were reduced in adult mice in which Hoxa deletion was induced. In the current study, we investigated the requirement of Hoxa genes at different stages of B cell development. Using an MxCre inducible conditional knock-out mouse model, we showed that immature B cell fractions and early lymphoid progenitors were markedly reduced in the absence of Hoxa whereas mature B cell populations were found at levels comparable to controls...
April 5, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28387990/cdh13-and-adipoq-gene-knockout-alters-instrumental-and-pavlovian-drug-conditioning
#11
Christopher P King, Leah Militello, Amy Hart, Celine L St Pierre, Emily Leung, Cassandra L Versaggi, Nathaniel Roberson, James Catlin, Abraham A Palmer, Jerry B Richards, Paul J Meyer
Genome-wide association studies in humans have suggested that variants of the cadherin-13 (CDH13) gene are associated with substance use disorder, subjective response to amphetamine, and attention deficit hyperactivity disorder. To examine the role of the Cdh13 and its peptide ligand adiponectin (AdipoQ) in addiction-related behaviors, we assessed Cdh13 knock-out rats and AdipoQ knock-out mice using intravenous cocaine self-administration and conditioned place preference paradigms. During intravenous cocaine self-administration, male Cdh13 heterozygous (+/-) and knock-out (-/-) rats showed increased cue-induced reinstatement compared to wild-type rats when presented with a cocaine-paired stimulus, whereas female Cdh13 rats showed no differences across genotype...
April 7, 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28382046/evidence-for-gaba-induced-systemic-gaba-accumulation-in-arabidopsis-upon-wounding
#12
Sandra S Scholz, Jaiana Malabarba, Michael Reichelt, Monika Heyer, Frank Ludewig, Axel Mithöfer
The non-proteinogenic amino acid γ-aminobutyric acid (GABA) is present in all plant species analyzed so far. Its synthesis is stimulated by either acidic conditions occurring after tissue disruption or higher cytosolic calcium level. In mammals, GABA acts as inhibitory neurotransmitter but its function in plants is still not well understood. Besides its involvement in abiotic stress resistance, GABA has a role in the jasmonate-independent defense against invertebrate pests. While the biochemical basis for GABA accumulation in wounded leaves is obvious, the underlying mechanisms for wounding-induced GABA accumulation in systemic leaves remained unclear...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28379360/core-genes-evolve-rapidly-in-the-long-term-evolution-experiment-with-escherichia-coli
#13
Rohan Maddamsetti, Philip J Hatcher, Anna G Green, Barry L Williams, Debora S Marks, Richard E Lenski
Bacteria can evolve rapidly under positive selection owing to their vast numbers, allowing their genes to diversify by adapting to different environments. We asked whether the same genes that evolve rapidly in the long-term evolution experiment with Escherichia coli (LTEE) have also diversified extensively in nature. To make this comparison, we identified ∼2000 core genes shared among 60 E. coli strains. During the LTEE, core genes accumulated significantly more nonsynonymous mutations than flexible (i.e...
April 4, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28376796/ahcoda-db-a-data-repository-with-web-based-mining-tools-for-the-analysis-of-automated-high-content-mouse-phenomics-data
#14
Bastijn Koopmans, August B Smit, Matthijs Verhage, Maarten Loos
BACKGROUND: Systematic, standardized and in-depth phenotyping and data analyses of rodent behaviour empowers gene-function studies, drug testing and therapy design. However, no data repositories are currently available for standardized quality control, data analysis and mining at the resolution of individual mice. DESCRIPTION: Here, we present AHCODA-DB, a public data repository with standardized quality control and exclusion criteria aimed to enhance robustness of data, enabled with web-based mining tools for the analysis of individually and group-wise collected mouse phenotypic data...
April 4, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28373017/conditional-postnatal-deletion-of-the-neonatal-murine-hepatic-circadian-gene-npas2-alters-the-gut-microbiome-following-restricted-feeding
#15
Derek S O'Neil, Christopher J Stewart, Derrick M Chu, Danielle M Goodspeed, Pablo J Gonzalez-Rodriguez, Cynthia D Shope, Kjersti M Aagaard
BACKGROUND: We have recently shown in both non-human primates and in rodents that fetal and neonatal hepatic expression of the circadian transcription factor, Npas2, is modulated by a high fat maternal diet and plays a critical role in establishing life-long metabolic homeostasis. Similarly, we and others have also established the importance of the maternal and early postnatal diet on establishment of the early gut microbiome. OBJECTIVE: We hypothesized that altered circadian gene expression solely in the neonatal liver would result in gut microbiome dysbiosis, especially with diet-induced metabolic stress (ie, restricted feeding)...
March 31, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28370799/through-modulation-of-cardiac-ca-2-handling-ucp2-affects-cardiac-electrophysiology-and-influences-the-susceptibility-for-ca-2-mediated-arrhythmias
#16
Robert Larbig, Sara Reda, Vera Paar, Andrea Trost, Johannes Leitner, Stephanie Weichselbaumer, Karolina A Motloch, Bernhard Wernly, Andreas Arrer, Benjamin Strauss, Michael Lichtenauer, Herbert A Reitsamer, Lars Eckardt, Guiscard Seebohm, Uta C Hoppe, Lukas J Motloch
Introduction UCP2 belongs to a superfamily of mitochondrial ion transporters. Due to its beneficial influence on production of reactive oxygen species it is suggested to reduce cardiac ischemic reperfusion injury. Recent studies uncovered its ability to regulate mitochondrial Ca(2+) -uptake and therefore to influence cardiac cytosolic Ca(2+) -handling, indicating compensatory pathways to avoid toxic Ca(2+) -overload in UCP2 knock-out mice (UCP2(-/-) ). However, the specific mechanisms and their impact on cardiac electrophysiology remain speculative...
March 31, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28369596/paralogs-and-mutants-show-that-one-dma-synthase-functions-in-fe-homeostasis-in-rice
#17
Khurram Bashir, Tomoko Nozoye, Seiji Nagasaka, Sultana Rasheed, Nanako Miyauchi, Motoaki Seki, Hiromi Nakanishi, Naoko K Nishizawa
Rice (Oryza sativa) secretes 2'-deoxymugineic acid (DMA) to acquire insoluble iron (Fe) from the rhizosphere. In rice, DMA is synthesized by DMA synthase 1 (OsDMAS1), a member of the aldo-keto reductase super family. We screened OsDMAS1 paralogs for DMA synthesis. None of these paralogs displayed in vitro DMA synthesis activity, suggesting that rice only harbors one functional DMAS. We further characterized OsDMAS1 mutant plants. We failed to screen homozygous knock-out plants (dmas-1), so we characterized DMAS knock-down plants (dmas-kd1 and dmas-kd2)...
March 28, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28363983/synaptotagmin2-syt2-drives-fast-release-redundantly-with-syt1-at-the-output-synapses-of-parvalbumin-expressing-inhibitory-neurons
#18
Brice Bouhours, Enida Gjoni, Olexiy Kochubey, Ralf Schneggenburger
Parvalbumin-expressing inhibitory neurons in the mammalian CNS are specialized for fast transmitter release at their output synapses. However, the Ca(2+) sensor(s) employed by identified inhibitory synapses, including the output synapses of PV-expressing inhibitory neurons, have only recently started to be addressed. Here, we investigated the roles of Syt1 and Syt2 at two types of fast-releasing inhibitory connections in the mammalian CNS, the MNTB to LSO glycinergic synapse, and the basket/stellate cell - Purkinje GABAergic synapse in the cerebellum...
March 31, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28363967/reexamination-of-the-physiological-role-of-pyka-in-escherichia-coli-revealed-that-it-negatively-regulates-the-intracellular-atp-levels-under-anaerobic-conditions
#19
Chunhua Zhao, Zhao Lin, Hongjun Dong, Yanping Zhang, Yin Li
Pyruvate kinase is one of the three rate-limiting glycolytic enzymes that catalyzes the last step of glycolysis, conversion of phosphoenolpyruvate (PEP) into pyruvate which is associated with ATP generation. Two isozymes of pyruvate kinase PykF and PykA are identified in Escherichia coli The PykF is considered important, whereas the PykA has less-defined role. Prior studies inactivated the pykA gene to increase the level of its substrate, PEP, and thereby increased the yield of end-products derived from PEP...
March 31, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28360124/genetic-mutations-linked-to-parkinson-s-disease-differentially-control-nucleolar-activity-in-pre-symptomatic-mouse-models
#20
V Evsyukov, A Domanskyi, H Bierhoff, S Gispert, R Mustafa, F Schlaudraff, B Liss, R Parlato
Genetic mutations underlying neurodegenerative disorders impair ribosomal DNA (rDNA) transcription suggesting nucleolar dysfunction as a novel pathomechanism in polyglutamine diseases and in certain forms of amyotrophic lateral sclerosis/frontotemporal dementia. Here, we investigated nucleolar activity in pre-symptomatic digenic models of Parkinson's disease (PD) modeling the multifactorial etiology of this disease.To this end, we analyzed a novel mouse model mildly overexpressing the mutant human-A53T-alpha-synuclein (hA53T-SNCA/PARK1) in a PTEN-induced kinase 1 (PINK1/PARK6) knock-out background and mutant mice lacking both DJ-1/PARK7 and PINK1/PARK6...
March 30, 2017: Disease Models & Mechanisms
keyword
keyword
40962
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"