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https://www.readbyqxmd.com/read/29312537/lipopolysaccharide-induces-acute-bursal-atrophy-in-broiler-chicks-by-activating-tlr4-mapk-nf-%C3%AE%C2%BAb-ap-1-signaling
#1
Abdur Rahman Ansari, Ning-Ya Li, Zhi-Jian Sun, Hai-Bo Huang, Xing Zhao, Lei Cui, Ya-Fang Hu, Ju-Ming Zhong, Niel A Karrow, Hua-Zhen Liu
We investigated the mechanisms that induce atrophy of the chicken bursa of Fabricius (BF) upon lipopolysaccharide (LPS) treatment in young chicks. LPS treatment resulted in ∼36% decrease in bursal weight within 36 h (P < 0.01). Histological analysis showed infiltration of eosinophilic heterophils and nucleated oval shaped RBCs in or near blood vessels of the BF from LPS-treated chicks. Scanning electron micrographs showed severe erosion and breaks in the mucosal membrane at 12 h and complete exuviation of bursal mucosal epithelial cells at 36 h...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29310608/epoxyazadiradione-suppresses-breast-tumor-growth-through-mitochondrial-depolarization-and-caspase-dependent-apoptosis-by-targeting-pi3k-akt-pathway
#2
Dhiraj Kumar, Saikat Haldar, Mahadeo Gorain, Santosh Kumar, Fayaj A Mulani, Amit S Yadav, Lucio Miele, Hirekodathakallu V Thulasiram, Gopal C Kundu
BACKGROUND: Breast cancer is one of the most commonly diagnosed invasive cancers among women around the world. Among several subtypes, triple negative breast cancer (TNBC) is highly aggressive and chemoresistant. Treatment of TNBC patients has been challenging due to heterogeneity and devoid of well-defined molecular targets. Thus, identification of novel effective and selective agents against TNBC is essential. METHODS: We used epoxyazadiradione to assess the cell viability, mitochondrial potential, ROS level, cell migration, apoptosis and protein expression in cell culture models of TNBC MDA-MB-231 and ER+ MCF-7 breast cancer cells...
January 8, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29305742/cxcl12-gene-silencing-down-regulates-metastatic-potential-via-blockage-of-mapk-pi3k-ap-1-signaling-pathway-in-colon-cancer
#3
J Ma, H Su, B Yu, T Guo, Z Gong, J Qi, X Zhao, J Du
BACKGROUND: To investigate the effect of CXCL12 gene silencing on proliferation,invasion, angiogenesis and the relationship of MAPK/PI3K/AP-1 signaling pathway in colon cancer cells. METHODS: RT-PCR and Western-blot were used to detect the expression of CXCL12 mRNA and protein in four colon cancer cell lines. Human colon cancer cells were transfected with CXCL12 siRNA carrying by Lipofectamine 2000. The expression of CXCL12 protein was confirmed by immunoblotting...
January 5, 2018: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/29305491/local-sequence-features-that-influence-ap-1-cis-regulatory-activity
#4
Hemangi G Chaudhari, Barak A Cohen
In the genome, most occurrences of transcription factor binding sites (TFBS) have no cis-regulatory activity, which suggests that flanking sequences contain information that distinguishes functional from non-functional TFBS. We interrogated the role of flanking sequences near Activator Protein 1 (AP-1) binding sites that reside in DNase I Hypersensitive Sites (DHS) and regions annotated as Enhancers. In these regions we found that sequence features directly adjacent to the core motif distinguish high from low activity AP-1 sites...
January 5, 2018: Genome Research
https://www.readbyqxmd.com/read/29305012/regulation-of-gene-transcription-following-stimulation-of-transient-receptor-potential-trp-channels
#5
Gerald Thiel, Andrea Lesch, Sandra Rubil, Tobias M Backes, Oliver G Rössler
Transient receptor potential (TRP) channels belong to a heterogeneous superfamily of cation channels that are involved in the regulation of numerous biological functions, including regulation of Ca2+ and glucose homeostasis, tumorigenesis, temperature, and pain sensation. To understand the functions of TRP channels, their associated intracellular signaling pathways and molecular targets have to be identified on the cellular level. Stimulation of TRP channels frequently induces an influx of Ca2+ ions into the cells and the subsequent activation of protein kinases...
2018: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/29296868/cord-blood-transplantation-recapitulates-fetal-ontogeny-with-a-distinct-molecular-signature-that-supports-cd4-t-cell-reconstitution
#6
Prashant Hiwarkar, Mike Hubank, Waseem Qasim, Robert Chiesa, Kimberly C Gilmour, Aurore Saudemont, Persis J Amrolia, Paul Veys
Omission of in vivo T-cell depletion promotes rapid, thymic-independent CD4+-biased T-cell recovery after cord blood transplant. This enhanced T-cell reconstitution differs from that seen after stem cell transplant from other stem cell sources, but the mechanism is not known. Here, we demonstrate that the transcription profile of naive CD4+ T cells from cord blood and that of lymphocytes reconstituting after cord blood transplantation is similar to the transcription profile of fetal CD4+ T cells. This profile is distinct to that of naive CD4+ T cells from peripheral blood and that of lymphocytes reconstituting after T-replete bone marrow transplantation...
November 14, 2017: Blood Advances
https://www.readbyqxmd.com/read/29295935/estrogen-dependent-epigenetic-regulation-of-soluble-epoxide-hydrolase-via-dna-methylation
#7
Yang-Ming Yang, Dong Sun, Sharath Kandhi, Ghezal Froogh, Jian Zhuge, Weihua Huang, Bruce D Hammock, An Huang
To elucidate molecular mechanisms responsible for the sexually dimorphic phenotype of soluble epoxide hydrolase (sEH) expression, we tested the hypothesis that female-specific down-regulation of sEH expression is driven by estrogen-dependent methylation of the Ephx2 gene. Mesenteric arteries isolated from male, female, ovariectomized female (OV), and OV with estrogen replacement (OVE) mice, as well as the human cell line (HEK293T) were used. Methylation-specific PCR and bisulfite genomic sequencing analysis indicate significant increases in DNA/CG methylation in vessels of female and OVE compared with those of male and OV mice...
January 2, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29290826/a-cell-penetrating-whole-molecule-antibody-targeting-intracellular-hbx-suppresses-hepatitis-b-virus-via-trim21-dependent-pathway
#8
Jun-Fang Zhang, Hua-Long Xiong, Jia-Li Cao, Shao-Juan Wang, Xue-Ran Guo, Bi-Yun Lin, Ying Zhang, Jing-Hua Zhao, Ying-Bin Wang, Tian-Ying Zhang, Quan Yuan, Jun Zhang, Ning-Shao Xia
Rationale: Monoclonal antibodies (mAbs) mostly targeting extracellular or cell surface molecules have been widely used in the treatment of various diseases. However, mAbs cannot pass through the cell membrane as efficiently as small compounds, thus limiting their use against intracellular targets. Methods to shuttle antibodies into living cells may largely expand research and application in areas based on mAbs. Hepatitis B virus X protein (HBx) is an important intracellular multi-functional viral protein in the life cycle of hepatitis B virus (HBV)...
2018: Theranostics
https://www.readbyqxmd.com/read/29287195/-6-shogaol-attenuates-inflammation-cell-proliferation-via-modulate-nf-%C3%AE%C2%BAb-and-ap-1-oncogenic-signaling-in-7-12-dimethylbenz-a-anthracene-induced-oral-carcinogenesis
#9
Govindhan Annamalai, Kathiresan Suresh
Nuclear factor-kappaB (NF-κB) and activator protein 1 (AP-1) is a major transcription factor which regulates many biological and pathological processes such as inflammation and cell proliferation, which are major implicates in cancer progression. [6]-Shogaol ([6]-SHO) is a major constituent of ginger, exhibits various biological properties such as anti-oxidants, anti-inflammation and anti-tumor. Recently, we proven that [6]-SHO prevents oral squamous cell carcinoma by activating proapoptotic factors in in vitro and in vivo experimental model...
December 26, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29285096/anti-inflammatory-effect-of-delphinidin-on-intramedullary-spinal-pressure-in-a-spinal-cord-injury-rat-model
#10
Cheng-Hu Wang, Lin-Lin Zhu, Ke-Feng Ju, Jin-Long Liu, Kun-Peng Li
Delphinidin, a flavonoid polyphenolic compound, is widely found in nature and is used as a food supplement due to its pharmacological activity. The aims of the present study were to examine the anti-inflammatory effect of delphinidin in alleviating spinal cord injury (SCI)-induced inflammation in a rat model and to determine the underlying mechanisms in SCI. The Basso, Beattie, Bresnahan (BBB) scores of rats were assessed to evaluate the effect of delphinidin on the recovery of motor function. ELISA kits were also used to analyze the activities of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2) and caspase-3...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29278651/ap1-mediates-upa-upar-induced-fut4-expression-and-trophoblast-invasion
#11
Qin Zheng, Yu Yang, Xinyuan Cui, Dandan Zhang, Shuai Liu, Qiu Yan
Placental trophoblast invasion is crucial for embryo implantation and successful pregnancy. Urokinase-type plasminogen activator (uPA)/urokinase-type plasminogen activator receptor (uPAR) are expressed on trophoblasts and involved in trophoblast invasion. The transcription factor activator protein 1 (AP-1) (c-FOS and c-JUN) and fucosyltransferase IV (FUT4) have been found to be involved in trophoblast invasion. However, the relationship of uPA/uPAR, AP1 and FUT4 is unclear in trophoblast invasion. The current study aimed to investigate the role of AP1 in uPA/uPAR-induced FUT4 expression and trophoblast invasion...
December 26, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29277228/molecular-mechanism-of-action-of-pelargonidin-3-o-glucoside-the-main-anthocyanin-responsible-for-the-anti-inflammatory-effect-of-strawberry-fruits
#12
Larissa Jeremias Duarte, Vitor Clasen Chaves, Marcus Vinicius Pereira Dos Santos Nascimento, Eunice Calvete, Mingchuan Li, Elisa Ciraolo, Alessandra Ghigo, Emilio Hirsch, Claudia Maria Oliveira Simões, Flávio Henrique Reginatto, Eduardo M Dalmarco
Fragaria x ananassa Duch., popularly called strawberry, is known for its worldwide consumption and important biological activities, and these effects are related to its high concentration of anthocyanins. Pelargonidin-3-O-glucoside (P3G) is a major anthocyanin found in strawberry, and was evaluated for its anti-inflammatory action in experimental models. The effect of strawberry extract and P3G, on leukocyte migration, exudation levels and many inflammatory mediators, was therefore evaluated in an in vivo model...
May 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29276516/myostatin-promotes-interleukin-1%C3%AE-expression-in-rheumatoid-arthritis-synovial-fibroblasts-through-inhibition-of-mir-21-5p
#13
Sung-Lin Hu, An-Chen Chang, Chien-Chung Huang, Chun-Hao Tsai, Cheng-Chieh Lin, Chih-Hsin Tang
Rheumatoid arthritis (RA) is characterized by the infiltration of a number of pro-inflammatory cytokines into synovial fluid and patients with RA often develop joint destruction and deficits in muscle mass. The growth factor myostatin is a key regulator linking muscle mass and bone structure. We sought to determine whether myostatin regulates rheumatoid synovial fibroblast activity and inflammation in RA. We found that levels of myostatin and interleukin (IL)-1β (a key pro-inflammatory cytokine in RA) in synovial fluid from RA patients were overexpressed and positively correlated...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29274289/epigenetic-regulation-of-integrin-%C3%AE-6-transcription-induced-by-tgf-%C3%AE-1-in-human-oral-squamous-cell-carcinoma-cells
#14
Mingyan Xu, Liqin Yin, Yihuang Cai, Qingwei Hu, Jie Huang, Qing Ji, Yanping Hu, Wenxia Huang, Fan Liu, Songlin Shi, Xiaoling Deng
Overexpression of integrin αvβ6 is believed to play an important role in the invasion and metastasis of oral squamous cell carcinoma (OSCC). However, little is known about the molecular mechanisms leading to αvβ6 upregualtion in OSCC. As the integrin β6 (ITGB6) is the only partner with αv, the expression of αvβ6 is dependent on ITGB6, it is therefore pivotal to investigate the mechanisms underlying ITGB6 overexpression in OSCC. We previously reported the cloning and characterization of human ITGB6 gene...
December 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29273799/rna-seq-identifies-a-diminished-differentiation-gene-signature-in-primary-monolayer-keratinocytes-grown-from-lesional-and-uninvolved-psoriatic-skin
#15
William R Swindell, Mrinal K Sarkar, Yun Liang, Xianying Xing, Jaymie Baliwag, James T Elder, Andrew Johnston, Nicole L Ward, Johann E Gudjonsson
Keratinocyte (KC) hyper-proliferation and epidermal thickening are characteristic features of psoriasis lesions, but the specific contributions of KCs to plaque formation are not fully understood. This study used RNA-seq to investigate the transcriptome of primary monolayer KC cultures grown from lesional (PP) and non-lesional (PN) biopsies of psoriasis patients and control subjects (NN). Whole skin biopsies from the same subjects were evaluated concurrently. RNA-seq analysis of whole skin identified a larger number of psoriasis-increased differentially expressed genes (DEGs), but analysis of KC cultures identified more PP- and PN-decreased DEGs...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29258818/remodeling-of-hiv-1-nef-structure-by-src-family-kinase-binding
#16
Jamie A Moroco, John Jeff Alvarado, Ryan P Staudt, Haibin Shic, Thomas E Wales, Thomas E Smithgall, John R Engen
The HIV-1 accessory protein Nef controls multiple aspects of the viral life cycle and host immune response, making it an attractive therapeutic target. Previous X-ray crystal structures of Nef in complex with key host cell binding partners have shed light on protein-protein interactions critical to Nef function. Crystal structures of Nef in complex with either the SH3 or tandem SH3-SH2 domains of Src-family kinases reveal distinct dimer conformations of Nef. However, the existence of these Nef dimer complexes in solution has not been established...
December 16, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29248584/dexamethasone-inhibits-inflammatory-response-via-down-regulation-of-ap-1-transcription-factor-in-human-lung-epithelial-cells
#17
Rajeshwari H Patil, M Naveen Kumar, K M Kiran Kumar, Rashmi Nagesh, K Kavya, R L Babu, Govindarajan T Ramesh, S Chidananda Sharma
The production of inflammatory mediators by epithelial cells in inflammatory lung diseases may represent an important target for the anti-inflammatory effects of glucocorticoids. Activator protein-1 (Khanapure et al.) is a major activator of inflammatory genes and has been proposed as a target for inhibition by glucocorticoids. We have used human pulmonary type-II A549 cells to examine the effect of dexamethasone on the phorbol ester (PMA)/Lipopolysaccharide (LPS) induced pro-inflammatory cytokines and AP-1 factors...
December 14, 2017: Gene
https://www.readbyqxmd.com/read/29248490/acetylation-regulates-the-mkk4-jnk-pathway-in-t-cell-receptor-signaling
#18
Yukihide Matsui, Taku Kuwabara, Toyonobu Eguchi, Koichi Nakajima, Motonari Kondo
T cell functions are regulated by multiple signaling cascades, including the MKK4-JNK (c-Jun NH2 terminal kinase) pathway. However, the mechanism regulating the MKK4-JNK axis in T cells remains unclear. Herein, we demonstrated that protein acetylation modulates JNK activity induced by T cell receptor (TCR) activation. The acetyltransferase, CREB-binding protein (CBP), is transported from the nucleus to the cytoplasm in response to TCR cross-linking. To investigate the role of CBP in TCR signaling, we overexpressed CBP in the cytoplasm of Jurkat cells, a human T lymphocyte line...
December 14, 2017: Immunology Letters
https://www.readbyqxmd.com/read/29246325/ap-1-oligodeoxynucleotides-reduce-aortic-elastolysis-in-a-murine-model-of-marfan-syndrome
#19
Rawa Arif, Marcin Zaradzki, Anca Remes, Philipp Seppelt, Reiner Kunze, Hannes Schröder, Simon Schwill, Stephan M Ensminger, Peter N Robinson, Matthias Karck, Oliver J Müller, Markus Hecker, Andreas H Wagner, Klaus Kallenbach
Marfan syndrome is characterized by high expression of matrix metalloproteinases (MMPs) in aortic smooth muscle cells (AoSMCs) associated with medial elastolysis and aortic root aneurysm. We aimed to reduce aortic elastolysis through decrease of MMP expression with decoy oligodeoxynucleotides (dODNs) neutralizing the transcription factor activating factor-1 (AP-1). AP-1 abundance in nuclear extracts as well as MMP-2 and MMP-9 expression were significantly increased in isolated mAoSMC of mgR/mgR Marfan mice compared to wild-type cells...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29245117/gold-nanoflowers-synthesized-using-acanthopanacis-cortex-extract-inhibit-inflammatory-mediators-in-lps-induced-raw264-7-macrophages-via-nf-%C3%AE%C2%BAb-and-ap-1-pathways
#20
Sungeun Ahn, Priyanka Singh, Mi Jang, Yu-Jin Kim, Verónica Castro-Aceituno, Shakina Yesmin Simu, Yeon Ju Kim, Deok-Chun Yang
We reported the rapid synthesis (<8s) of gold nanoparticles at room temperature using Acanthopanacis cortex extract (A-AuNPs). We characterized the A-AuNPs using several analytical techniques and found that nano-flower type A-AuNPs, which are known to possess a coarse surface with a high surface to volume ratio, conferring these particles with high binding capacity for various biological molecules. After confirming the stability of the nanoparticles, we investigated the anti-inflammatory effect of A-AuNPs in LPS-stimulated RAW264...
November 17, 2017: Colloids and Surfaces. B, Biointerfaces
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