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Spermiogenesis

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https://www.readbyqxmd.com/read/28727099/identification-of-differentially-expressed-proteins-in-testicular-semen-of-sex-reversed-female-xx-and-normal-male-xy-rainbow-trout
#1
J Nynca, M Adamek, A Ciereszko
Masculinized females, named sex-reversed females (SRF), have a male phenotype but retain the female genotype (XX) and all spermatozoa produced in their testes carry the X chromosome. Masculinization of females leads to incomplete testicular development and the production of lower-quality semen. The mechanism of masculinization is unknown at present. Therefore, the aim of our study was to identify differentially abundant proteins in testicular semen of normal males and SRF using a difference in-gel electrophoresis approach...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28709083/the-peculiar-extra-acrosomal-structure-of-the-collembola-hexapoda-spermatozoa
#2
P P Fanciulli, C Mencarelli, D Mercati, R Dallai, P Lupetti
The springtail Collembola are characterized by having rolled spermatozoa, with a long cylindrical extracellular structure adhering to the acrosome. This structure is produced by the secretory activity of the testes epithelial cells at almost the end of spermiogenesis. At the beginning of its formation, it is a thin extension with a helical wall and a dense axial region. Later the cylindrical structure shows an inner organization which is different in the several species examined: species of Entomobryidae contain material with a paracrystalline structure, whilst some of Symphypleona contain ovoid structures...
June 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28694462/transgenerational-inheritance-of-susceptibility-to-diabetes-induced-male-subfertility
#3
Gabriela Pavlinkova, Hasmik Margaryan, Eva Zatecka, Eliska Valaskova, Fatima Elzeinova, Alena Kubatova, Romana Bohuslavova, Jana Peknicova
Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694333/epc1-tip60-mediated-histone-acetylation-facilitates-spermiogenesis-in-mice
#4
Yixin Dong, Kyo-Ichi Isono, Kazuyuki Ohbo, Takaho A Endo, Osamu Ohara, Mamiko Maekawa, Yoshiro Toyama, Chizuru Ito, Kiyotaka Toshimori, Kristian Helin, Narumi Ogonuki, Kimiko Inoue, Atsuo Ogura, Kazutsune Yamagata, Issay Kitabayashi, Haruhiko Koseki
Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation-mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian Nucleosome acetyltransferase of H4 (NuA4) complexes, are co-expressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development...
July 10, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28689636/dynamic-transcription-and-expression-patterns-of-kif3a-and-kif3b-genes-during-spermiogenesis-in-the-shrimp-palaemon-carincauda
#5
Yong-Qiang Zhao, Hai-Yan Yang, Dan-Dan Zhang, Ying-Li Han, Cong-Cong Hou, Jun-Quan Zhu
Spermiogenesis is a highly ordered and complex process in the male germ cell differentiation. The microtubule-based motor proteins KIF3A and KIF3B are required for the progression of the stages of spermiogenesis. In this study, the main goal was to determine whether KIF3A and KIF3B have a key role in spermiogenesis in Palaemon carincauda. The complete cDNA of KIF3A/3B from the testis of P. carincauda was cloned by using PCR and rapid amplification of cDNA ends (RACE). The predicted secondary and tertiary structures of KIF3A/3B contained three domains which were the: a) head region, b) stalk region, and c) tail region...
July 1, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28689229/detection-of-candidate-nectin-gene-mutations-in-infertile-men-with-severe-teratospermia
#6
Richard Bronson, Anatoly Mikhailik, John Schwedes, Dimitri Gnatenko, Eli Hatchwell
PURPOSE: Approximately 40% of infertile men have an abnormal semen analysis, resulting from either abnormalities of sperm production (defective spermatogenesis) or sperm shape (defective spermiogenesis). This latter process is dependent upon the function of Sertoli cells, which maintain specialized junctional complexes with germ cells. Nectins, members of the immunoglobulin superfamily, participate in formation of these dynamic complexes. Male mice in which the nectin-2 or nectin-3 gene is knocked out are sterile...
July 8, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28684548/lem-domain-proteins-are-lost-during-human-spermiogenesis-but-baf-and-baf-l-persist
#7
Razan A Elkhatib, Marine Paci, Romain Boissier, Guy Longepied, Yasmina Auguste, Vincent Achard, Patrice Bourgeois, Nicolas Levy, Nicolas Branger, Michael J Mitchell, Catherine Metzler-Guillemain
During spermiogenesis the spermatid nucleus is elongated, and dramatically reduced in size with protamines replacing histones to produce a highly compacted chromatin. After fertilisation, this process is reversed in the oocyte to form the male pronucleus. Emerging evidence, including the coordinated loss of the nuclear lamina (NL) and the histones, supports the involvement of the NL in spermatid nuclear remodelling, but how the NL links to the chromatin is not known. In somatic cells, interactions between the NL and the chromatin have been demonstrated: LEM-domain proteins and LBR interact with the NL and, respectively, the chromatin proteins BAF and HP1...
July 6, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28669756/a-splice-variant-of-centrosomin-converts-mitochondria-to-microtubule-organizing-centers
#8
Jieyan V Chen, Rebecca A Buchwalter, Ling-Rong Kao, Timothy L Megraw
Non-centrosomal microtubule organizing centers (MTOCs) direct microtubule (MT) organization to exert diverse cell-type-specific functions. In Drosophila spermatids, the giant mitochondria provide structural platforms for MT reorganization to support elongation of the extremely long sperm. However, the molecular basis for this mitochondrial MTOC and other non-centrosomal MTOCs has not been discerned. Here we report that Drosophila centrosomin (cnn) expresses two major protein variants: the centrosomal form (CnnC) and a non-centrosomal form in testes (CnnT)...
July 10, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28662655/identification-characterization-and-purification-of-porcine-quiescin-q6-sulfydryl-oxidase-2-protein
#9
Yu-Wen Kuo, Radhika Joshi, Tse-En Wang, Hui-Wen Chang, Sheng-Hsiang Li, Chun-Ni Hsiao, Pei-Shiue Jason Tsai
BACKGROUND: Post-spermiogenesis membrane surface modifications rely on molecules present in the reproductive tracts. Two isoforms (isoform 1 and 2) from Quiescin Q6-Sulfydryl Oxidase protein family have been identified in the male reproductive tract of rodent species. However, unlike isoform 1, scarce information is available for isoform 2, likely due to its lower expression level and lack of proper purification methods to obtain sufficient protein quantity for further assays. RESULTS: This study demonstrated the presence of short and long forms of Quiescin Q6-Sulfydryl Oxidase 2 in boar, likely representing the secretory (short form) and transmembrane (long form) forms of Quiescin Q6-Sulfydryl Oxidase 2...
June 29, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28639134/kifc1-and-myosin-va-two-motors-for-acrosomal-biogenesis-and-nuclear-shaping-during-spermiogenesis-of-portunus-trituberculatus
#10
Dan-Dan Ma, Meng-Ying Pan, Cong-Cong Hou, Fu-Qing Tan, Wan-Xi Yang
To investigate the molecular mechanisms underlying the spermiogenesis of the swimming crab Portunus trituberculatus, full lengths of motor proteins KIFC1 and myosin Va were cloned by rapid-amplification of cDNA ends from P. trituberculatus testes cDNA, and their respective probes and specific antibodies were used to track their localization during sperm maturation. Antisense probes were designed from the gene sequences and used to detect the mRNA levels of each gene. According to the results of fluorescence in situ hybridization (FISH), the transcription of kifc1 and myosin Va began at the mid-stage of spermatids, with the kifc1 mRNA being most active at the location where the acrosome cap was formed and the myosin Va was more concentrated in the acrosome complex...
June 21, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28623263/probing-spermiogenesis-a-digital-strategy-for-mouse-acrosome-classification
#11
Alessandro Taloni, Francesc Font-Clos, Luca Guidetti, Simone Milan, Miriam Ascagni, Chiara Vasco, Maria Enrica Pasini, Maria Rosa Gioria, Emilio Ciusani, Stefano Zapperi, Caterina A M La Porta
Classification of morphological features in biological samples is usually performed by a trained eye but the increasing amount of available digital images calls for semi-automatic classification techniques. Here we explore this possibility in the context of acrosome morphological analysis during spermiogenesis. Our method combines feature extraction from three dimensional reconstruction of confocal images with principal component analysis and machine learning. The method could be particularly useful in cases where the amount of data does not allow for a direct inspection by trained eye...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28621664/post-meiotic-dna-double-strand-breaks-occur-in-tetrahymena-and-require-topoisomerase-ii-and-spo11
#12
Takahiko Akematsu, Yasuhiro Fukuda, Jyoti Garg, Jeffrey S Fillingham, Ronald E Pearlman, Josef Loidl
Based on observations of markers for DNA lesions, such as phosphorylated histone H2AX (γH2AX) and open DNA ends, it has been suggested that post-meiotic DNA double-strand breaks (PM-DSBs) enable chromatin remodeling during animal spermiogenesis. However, the existence of PM-DSBs is unconfirmed, and the mechanism responsible for their formation is unclear. Here, we report the first direct observation of programmed PM-DSBs via the electrophoretic separation of DSB-generated DNA fragments in the ciliate Tetrahymena thermophila...
June 16, 2017: ELife
https://www.readbyqxmd.com/read/28615530/in-vivo-autophagy-and-biogenesis-of-autophagosomes-within-male-haploid-cells-during-spermiogenesis
#13
Ping Yang, Nisar Ahmed, Lingling Wang, Hong Chen, Yasir Waqas, Tengfei Liu, Abdul Haseeb, Nasrullah Bangulzai, Yufei Huang, Qiusheng Chen
Autophagy is a unique catabolic pathway that is linked to several physiological processes. However, its role in the process of spermiogenesis is largely unknown. The aim of the current study was to determine the in vivo role of autophagy and the origin of autophagosome membrane biogenesis within male haploid cells. Our immunohistochemistry results demonstrated that LC3 and ATG7 localization were increased dramatically in round to elongated spermatids (haploid cells) towards the lumen of seminiferous tubules, however, poorly expressed in the early stages of germ cells near the basal membrane...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28615029/micrornas-control-mrna-fate-by-compartmentalization-based-on-3-utr-length-in-male-germ-cells
#14
Ying Zhang, Chong Tang, Tian Yu, Ruirui Zhang, Huili Zheng, Wei Yan
BACKGROUND: Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying post-transcriptional regulation in vivo because it involves a large number of transcripts that are physically sequestered in ribonucleoprotein particles (RNPs) and thus subjected to delayed translation...
June 15, 2017: Genome Biology
https://www.readbyqxmd.com/read/28607498/male-factor-infertility-piwi-hiwi-miwi-essential-genes-for-effective-spermiogenesis
#15
Annette Fenner
No abstract text is available yet for this article.
June 13, 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/28559472/spermatogonial-deubiquitinase-usp9x-is-essential-for-proper-spermatogenesis-in-mice
#16
Kasane Kishi, Aya Uchida, Hinako M Takase, Hitomi Suzuki, Masamichi Kurohmaru, Naoki Tsunekawa, Masami Kanai-Azuma, Stephen A Wood, Yoshiakira Kanai
USP9X (ubiquitin-specific peptidase 9, X chromosome) is the mammalian orthologue of Drosophila deubiquitinase fat facets that was previously shown to regulate the maintenance of the germ cell lineage partially through stabilizing Vasa, one of the widely conserved factors crucial for gametogenesis. Here, we demonstrate that USP9X is expressed in the gonocytes and spermatogonia in mouse testes from newborn to adult stages. By using Vasa-Cre mice, germ cell-specific conditional deletion of Usp9x from the embryonic stage showed no abnormality in the developing testes by 1 week and no appreciable defects in the undifferentiated and differentiating spermatogonia at postnatal and adult stages...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28552346/ubiquitination-deficient-mutations-in-human-piwi-cause-male-infertility-by-impairing-histone-to-protamine-exchange-during-spermiogenesis
#17
Lan-Tao Gou, Jun-Yan Kang, Peng Dai, Xin Wang, Feng Li, Shuang Zhao, Man Zhang, Min-Min Hua, Yi Lu, Yong Zhu, Zheng Li, Hong Chen, Li-Gang Wu, Dangsheng Li, Xiang-Dong Fu, Jinsong Li, Hui-Juan Shi, Mo-Fang Liu
Genetic studies have elucidated critical roles of Piwi proteins in germline development in animals, but whether Piwi is an actual disease gene in human infertility remains unknown. We report germline mutations in human Piwi (Hiwi) in patients with azoospermia that prevent its ubiquitination and degradation. By modeling such mutations in Piwi (Miwi) knockin mice, we demonstrate that the genetic defects are directly responsible for male infertility. Mechanistically, we show that MIWI binds the histone ubiquitin ligase RNF8 in a Piwi-interacting RNA (piRNA)-independent manner, and MIWI stabilization sequesters RNF8 in the cytoplasm of late spermatids...
June 1, 2017: Cell
https://www.readbyqxmd.com/read/28534180/the-c-terminal-kinesin-motor-kifc1-may-participate-in-nuclear-reshaping-and-flagellum-formation-during-spermiogenesis-of-larimichthys-crocea
#18
Dan-Dan Zhang, Xin-Ming Gao, Yong-Qiang Zhao, Cong-Cong Hou, Jun-Quan Zhu
Spermatogenesis is a highly ordered process in the differentiation of male germ cells. Nuclear morphogenesis is one of the most fundamental cellular transformations to take place during spermatogenesis. These striking transformations from spermatogonia to spermatozoa are a result of phase-specific adaption of the cytoskeleton and its association with molecular motor proteins. KIFC1 is a C-terminal kinesin motor protein that plays an essential role in acrosome formation and nuclear reshaping during spermiogenesis in mammals...
May 23, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28500503/expression-and-localization-of-myosin-vi-in-developing-mouse-spermatids
#19
Przemysław Zakrzewski, Robert Lenartowski, Maria Jolanta Rędowicz, Kathryn G Miller, Marta Lenartowska
Myosin VI (MVI) is a versatile actin-based motor protein that has been implicated in a variety of different cellular processes, including endo- and exocytic vesicle trafficking, Golgi morphology, and actin structure stabilization. A role for MVI in crucial actin-based processes involved in sperm maturation was demonstrated in Drosophila. Because of the prominence and importance of actin structures in mammalian spermiogenesis, we investigated whether MVI was associated with actin-mediated maturation events in mammals...
May 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28476888/loss-of-the-na-h-exchanger-nhe8-causes-male-infertility-in-mice-by-disrupting-acrosome-formation
#20
Karina Oberheide, Dmytro Puchkov, Thomas J Jentsch
Mammalian sperm feature a specialized secretory organelle on the anterior part of the sperm nucleus, the acrosome, which is essential for male fertility. It is formed by a fusion of Golgi-derived vesicles. We show here that the predominantly Golgi-resident Na(+)/H(+) exchanger NHE8 localizes to the developing acrosome of spermatids. Similar to wild-type mice, Nhe8(-/-) mice generated Golgi-derived vesicles positive for acrosomal markers and attached to nuclei, but these vesicles failed to form large acrosomal granules and the acrosomal cap...
June 30, 2017: Journal of Biological Chemistry
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