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Spermiogenesis

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https://www.readbyqxmd.com/read/28415605/kifc1-is-essential-for-acrosome-formation-and-nuclear-shaping-during-spermiogenesis-in-the-lobster-procambarus-clarkii
#1
Dan-Dan Ma, Lian Bi, Wan-Xi Yang
In order to study the function of kinesin-14 motor protein KIFC1 during spermatogenesis of Procambarus clarkii, the full length of kifc1 was cloned from testes cDNA using Rapid-Amplification of cDNA Ends (RACE). The deduced KIFC1 protein sequence showed the highest similarity between Procambarus clarkii and Eriocheir senensis (similarity rate as 64%). According to the results of in situ hybridization (ISH), the kifc1 mRNA was gathered in the acrosome location above nucleus in the mid- and late-stage spermatids...
March 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28412438/torsional-stress-promotes-trinucleotidic-expansion-in-spermatids
#2
Olivier Simard, Seyedeh Raheleh Niavarani, Virginie Gaudreault, Guylain Boissonneault
Trinucleotide repeats are involved in various neurodegenerative diseases and are highly unstable both in dividing or non-dividing cells. In Huntington disease (HD), the age of onset of symptoms is inversely correlated to the number of CAG repeats within exon 1 of the HTT gene. HD shows paternal anticipation as CAG repeats are increased during spermatogenesis. CAG expansion were indeed found to be generated during the chromatin remodeling in spermatids where most histones are evicted and replaced by protamines...
April 9, 2017: Mutation Research
https://www.readbyqxmd.com/read/28389581/tssk6-is-required-for-%C3%AE-h2ax-formation-and-the-histone-to-protamine-transition-during-spermiogenesis
#3
Kula N Jha, Swamy K Tripurani, Gibbes R Johnson
Spermiogenesis includes transcriptional silencing, chromatin condensation and extensive morphological changes as spermatids transform into sperm. Chromatin condensation involves histone hyperacetylation, transitory DNA breaks, histone H2AX Ser139 phosphorylation (γH2AX), and replacement of histones by protamines. Previously, we reported that the spermatid protein kinase TSSK6 is essential for fertility in mice, but its specific role in spermiogenesis is unknown. Here, we show that TSSK6 expression is spatiotemporally coincident with γH2AX formation in nuclei of developing mouse spermatids...
April 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28388629/a-tubulin-alpha-8-mouse-knockout-model-indicates-a-likely-role-in-spermatogenesis-but-not-in-brain-development
#4
Christine P Diggle, Isabel Martinez-Garay, Zoltan Molnar, Martin H Brinkworth, Ed White, Ewan Fowler, Ruth Hughes, Bruce E Hayward, Ian M Carr, Christopher M Watson, Laura Crinnion, Aruna Asipu, Ben Woodman, P Louise Coletta, Alexander F Markham, T Neil Dear, David T Bonthron, Michelle Peckham, Ewan E Morrison, Eamonn Sheridan
Tubulin alpha 8 (Tuba8) is the most divergent member of the highly conserved alpha tubulin family, and uniquely lacks two key post-translational modification sites. It is abundantly expressed in testis and muscle, with lower levels in the brain. We previously identified homozygous hypomorphic TUBA8 mutations in human subjects with a polymicrogyria (PMG) syndrome, suggesting its involvement in development of the cerebral cortex. We have now generated and characterized a Tuba8 knockout mouse model. Homozygous mice were confirmed to lack Tuba8 protein in the testis, but did not display PMG and appeared to be neurologically normal...
2017: PloS One
https://www.readbyqxmd.com/read/28387711/descriptive-analysis-of-lap1-distribution-and-that-of-associated-proteins-throughout-spermatogenesis
#5
Joana B Serrano, Filipa Martins, João C Sousa, Cátia D Pereira, Ans M M van Pelt, Sandra Rebelo, Odete A B da Cruz E Silva
Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time...
April 7, 2017: Membranes
https://www.readbyqxmd.com/read/28387373/identification-of-a-variant-specific-phosphorylation-of-th2a-during-spermiogenesis
#6
Masashi Hada, Koji Masuda, Kosuke Yamaguchi, Katsuhiko Shirahige, Yuki Okada
Tissue-specific histone variant incorporation into chromatin plays dynamic and important roles in tissue development. Testis is one such tissue, and a number of testis-specific histone variants are expressed that have unique roles. While it is expected that such variants acquire post-transcriptional modifications to be functional, identification of variant-specific histone modifications is challenging because of the high similarity of amino acid sequences between canonical and variant versions. Here we identified a novel phosphorylation on TH2A, a germ cell-specific histone H2A variant...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28369153/detailed-analysis-of-the-male-reproductive-system-in-a-potential-bio-indicator-species-the-marine-invertebrate-galeolaria-caespitosa-polychaeta-serpulidae
#7
Yonggang Lu, Robert John Aitken, Minjie Lin
For the first time, this study has systemically investigated the male reproductive system in a sessile broadcast-spawning marine invertebrate, Galeolaria caespitosa (Polychaeta: Serpulidae), which has significant potential as a bio-indicator species of coastal marine pollution. The abdomen of G. caespitosa was divided by intersegmental septa into over 80 trunk segments. Each segment served as a germinal chamber with a C-shaped gonadal arrangement consisting of several distinct compartments: a seminiferous epithelium (SE) compartment located in the centre of the chamber, with each of its two ends connecting to a nurse cell (NC) compartment and then an efferent duct (ED) compartment...
2017: PloS One
https://www.readbyqxmd.com/read/28361989/testis-transcriptome-modulation-in-klinefelter-patients-with-hypospermatogenesis
#8
Marco D'Aurora, Alberto Ferlin, Andrea Garolla, Sara Franchi, Laura D'Onofrio, Oriana Trubiani, Giandomenico Palka, Carlo Foresta, Liborio Stuppia, Valentina Gatta
The main genetic cause of male infertility is represented by the Klinefelter Syndrome (KS), a condition accounting for 3% of all cases of infertility and up to15% of cases of azoospermia. KS is generally characterized by azoospermia; approximately 10% of cases have severe oligozoospermia. Among these, the 30-40% of patients show hypospermatogenesis. The mechanisms leading to adult testis dysfunctions are not completely understood. A microarray transcriptome analysis was performed on testis biopsies obtained from three KS patients with hypospermatogenesis and three control subjects...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28347860/ultrastructure-of-spermiogenesis-and-spermatozoa-in-marchalina-hellenica-gennadius-hemiptera-sternorrhyncha-marchalinidae
#9
R Dallai, Mercati D, S Gounari, F Paoli, P Lupetti
The spermiogenesis, the sperm structure and the sperm motility of Marchalina hellenica (Gennadius) were examined. In the early spermiogenesis a centriolar apparatus was identified, but this structure is not involved in the production of the sperm flagellum. As in other Coccoidea, the flagellar axoneme originates by the activity of the thickened tip of the numerous microtubules surrounding the nuclear anterior region close to the periphery of the cell. This region pushes against a narrow cytoplasmic layer, giving rise to a papilla...
March 24, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/28339613/a-nonsense-mutation-in-ccdc62-gene-is-responsible-for-spermiogenesis-defects-and-male-infertility-in-repro29-repro29-mice-%C3%A2
#10
Yuchi Li, Cailing Li, Shouren Lin, Bo Yang, Weiren Huang, Hanwei Wu, Yuanbin Chen, Lihua Yang, Manling Luo, Huan Guo, Jianbo Chen, Tiantian Wang, Qian Ma, Yanli Gu, Lisha Mou, Zhimao Jiang, Jun Xia, Yaoting Gui
Phenotype-driven mutagenesis is an unbiased method to identify novel genes involved in spermatogenesis and other reproductive processes. Male repro29/repro29 mice generated by the Reproductive Genomics Program at the Jackson Laboratory were infertile with deformed sperm and poor motility. Using selected exonic capture and massively parallel sequencing technologies, we identified a nonsense mutation in the exon 6 of coiled-coil domain-containing 62 gene (Ccdc62), which results in a formation of a premature stop codon and a truncated protein...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28298310/atypical-centrioles-are-present-in-tribolium-sperm
#11
E L Fishman, Kyoung Jo, Andrew Ha, Rachel Royfman, Ashtyn Zinn, Malathi Krishnamurthy, Tomer Avidor-Reiss
Typical centrioles are made of microtubules organized in ninefold symmetry. Most animal somatic cells have two centrioles for normal cell division and function. These centrioles originate from the zygote, but because the oocyte does not provide any centrioles, it is surprising that the zygotes of many animals are thought to inherit only one centriole from the sperm. Recently, in the sperm of Drosophila melanogaster, we discovered a second centriolar structure, the proximal centriole-like structure (PCL), which functions in the zygote...
March 2017: Open Biology
https://www.readbyqxmd.com/read/28297559/molecular-changes-and-signaling-events-occurring-in-spermatozoa-during-epididymal-maturation
#12
REVIEW
M G Gervasi, P E Visconti
After leaving the testis, spermatozoa have not yet acquired the ability to move progressively and are unable to fertilize oocytes. To become fertilization competent, they must go through an epididymal maturation process in the male, and capacitation in the female tract. Epididymal maturation can be defined as those changes occurring to spermatozoa in the epididymis that render the spermatozoa the ability to capacitate in the female tract. As part of this process, sperm cells undergo a series of biochemical and physiological changes that require incorporation of new molecules derived from the epididymal epithelium, as well as post-translational modifications of endogenous proteins synthesized during spermiogenesis in the testis...
March 2017: Andrology
https://www.readbyqxmd.com/read/28293398/nicotine-induced-damages-in-testicular-tissue-of-rats-evidences-for-bcl-2-p53-and-caspase-3-expression
#13
Maryam Mosadegh, Shapour Hasanzadeh, Mazdak Razi
OBJECTIVES: Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW(-1), IP) nicotine-received and high dose (0.4 mg/kg BW(-1), IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28292424/btbd18-regulates-a-subset-of-pirna-generating-loci-through-transcription-elongation-in-mice
#14
Liquan Zhou, Bertram Canagarajah, Yangu Zhao, Boris Baibakov, Keizo Tokuhiro, Dragan Maric, Jurrien Dean
PIWI-interacting RNAs (piRNAs) are small non-coding RNAs essential for animal germ cell development. Despite intense investigation of post-transcriptional processing, chromatin regulators for piRNA biogenesis in mammals remain largely unexplored. Here we document that BTBD18 is a pachytene nuclear protein in mouse testes that occupies a subset of pachytene piRNA-producing loci. Ablation of Btbd18 in mice disrupts piRNA biogenesis, prevents spermiogenesis, and results in male sterility. Transcriptome profiling, chromatin accessibility, and RNA polymerase II occupancy demonstrate that BTBD18 facilitates expression of pachytene piRNA precursors by promoting transcription elongation...
March 13, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28266557/heterozygous-mutation-of-eef1a1b-resulted-in-spermatogenesis-arrest-and-infertility-in-male-tilapia-oreochromis-niloticus
#15
Jinlin Chen, Dongneng Jiang, Dejie Tan, Zheng Fan, Yingying Wei, Minghui Li, Deshou Wang
Eukaryotic elongation factor 1 alpha (eEF1A) is an essential component of the translational apparatus. In the present study, eEF1A1b was isolated from the Nile tilapia. Real-time PCR and Western blot revealed that eEF1A1b was expressed highly in the testis from 90 dah (days after hatching) onwards. In situ hybridization and immunohistochemistry analyses showed that eEF1A1b was highly expressed in the spermatogonia of the testis. CRISPR/Cas9 mediated mutation of eEF1A1b resulted in spermatogenesis arrest and infertility in the F0 XY fish...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28261828/transformation-migration-and-outcome-of-residual-bodies-in-the-seminiferous-tubules-of-the-rat-testis
#16
C-Y Xiao, Y-Q Wang, J-H Li, G-C Tang, S-S Xiao
Experiments were performed to study the transformation, migration and outcome of residual bodies (RBs) in the seminiferous tubules of the rat testes. One part of the testes from adult Sprague-Dawley rats was used to generate paraffin sections to observe RBs and RB precursors through specific staining, and the other part of the testes was used to generate ultrathin sections to observe RBs under a transmission electron microscope. Deep blue particles of different sizes were observed in some seminiferous tubules through specific staining for RBs and RB precursors...
March 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28248003/delayed-sexual-maturity-in-males-of-vespa-velutina
#17
Juliette Poidatz, Christophe Bressac, Olivier Bonnard, Denis Thiéry
Vespa velutina var nigrithorax (lepelletier, 1835) is an invasive predator of bees accidentally introduced in France in 2004, and it is having a serious impact on apiculture and ecosystems. Studying the reproduction of an invasive species is key to assess its population dynamic. This study explores the sexual maturation of V. velutina males and the evolution of their fertility. The main studied parameters were physiologic (spermiogenesis, spermatogenesis) and anatomic (testes size and structure, head width)...
March 1, 2017: Insect Science
https://www.readbyqxmd.com/read/28242104/structural-features-of-the-spermatozoon-of-a-passeridan-bird-the-carib-grackle-quiscalus-lugubris
#18
Tom A Aire, Lizette du Plessis, Mahesh S Deokar, Eugene Rennie, Sunil K Gupta
The spermatozoon of the Carib grackle, Quiscalus lugubris, a member of the family Icteridae, is generally similar in organization to the passerine-type of spermatozoon, in being highly elongated and displaying a helical structure of the acrosome, nucleus and principal piece of the tail. There are subtle variations in acrosomal structural features between this organelle in the grackle and that in some of the very few passerine species of birds in which the spermatozoon has been studied. The proximal centriole is present, and, thus, the Carib grackle is the third passeridan bird in which this organelle, hitherto regarded as absent in passerine birds, has been described in the spermatozoon...
April 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28241054/manchette-acrosome-disorders-during-spermiogenesis-and-low-efficiency-of-seminiferous-tubules-in-hypercholesterolemic-rabbit-model
#19
Layla Simón, Abi K Funes, Martín A Yapur, María E Cabrillana, María A Monclus, Paola V Boarelli, Amanda E Vincenti, Tania E Saez Lancellotti, Miguel W Fornés
Hypercholesterolemia is a marker for several adult chronic diseases. Recently we demonstrated that sub/infertility is also associated to Hypercholesterolemia in rabbits. Seminal alterations included: abnormal sperm morphology, decreased sperm number and declined percentage of motile sperm, among others. In this work, our objective was to evaluate the effects of hypercholesterolemia on testicular efficiency and spermiogenesis, as the latter are directly related to sperm number and morphology respectively. Tubular efficiency was determined by comparing total number of spermatogenic cells with each cell type within the proliferation/differentiation compartments...
2017: PloS One
https://www.readbyqxmd.com/read/28235844/tbrd-1-and-tbrd-2-regulate-expression-of-genes-necessary-for-spermatid-differentiation
#20
Ina Theofel, Marek Bartkuhn, Thomas Boettger, Stefanie M K Gärtner, Judith Kreher, Alexander Brehm, Christina Rathke
Male germ cell differentiation proceeds to a large extent in the absence of active gene transcription. In Drosophila, hundreds of genes whose proteins are required during post-meiotic spermatid differentiation (spermiogenesis) are transcribed in primary spermatocytes. Transcription of these genes depends on the sequential action of the testis meiotic arrest complex (tMAC), Mediator complex, and testis-specific TFIID (tTFIID) complex. How the action of these protein complexes is coordinated and which other factors are involved in the regulation of transcription in spermatocytes is not well understood...
April 15, 2017: Biology Open
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