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Spermiogenesis

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https://www.readbyqxmd.com/read/27920259/hormone-sensitive-lipase-deficiency-alters-gene-expression-and-cholesterol-content-of-mouse-testis
#1
Feng Wang, Zheng Chen, Xiaofang Ren, Ye Tian, Fucheng Wang, Chao Liu, Pengcheng Jin, Zongyue Li, Feixiong Zhang, Baochang Zhu
Hormone-sensitive lipase-knockout (HSL-/-) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes...
February 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27905325/the-impact-of-autophagy-in-spermiogenesis
#2
Nihan Ozturk, Klaus Steger, Undraga Schagdarsurengin
No abstract text is available yet for this article.
November 29, 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27896558/h3k9ac-involved-in-the-decondensation-of-spermatozoal-nuclei-during-spermatogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#3
Genliang Li, Xianjiang Kang, Shumei Mu, Mingshen Guo, Shiwen Huang, Qinna Chen, Song Nong, Xiaomin Huang, Hongliu Hu, Ke Sun
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27862212/comparative-morphology-of-the-reproductive-system-of-seven-species-of-ostariophysan-fishes-from-the-upper-das-velhas-river-brazil
#4
Rafael Magno Costa Melo, Débora Diniz Gomes, Davidson Peruci Moreira, Maysa Regina Gomes, Nilo Bazzoli, Elizete Rizzo
The success of fishes in different environments is related with the variation of reproductive strategies developed by the systematic group, which is reflected in the morphology of the reproductive system and can have ecological and evolutionary implications. This study comparatively analyzed the morphological characteristics of the male and female reproductive systems of ostariophysan fish species from the upper Das Velhas River in the São Francisco River basin, Brazil. In order to accomplish this, 393 specimens belonging to seven fish species were sampled between April 2010 and June 2015 for histological, ultrastructural, histochemical, and morphometric analyses...
November 8, 2016: Journal of Morphology
https://www.readbyqxmd.com/read/27854341/sept12-ndc1-complexes-are-required-for-mammalian-spermiogenesis
#5
Tsung-Hsuan Lai, Ying-Yu Wu, Ya-Yun Wang, Mei-Feng Chen, Pei Wang, Tsung-Ming Chen, Yi-No Wu, Han-Sun Chiang, Pao-Lin Kuo, Ying-Hung Lin
Male factor infertility accounts for approximately 50 percent of infertile couples. The male factor-related causes of intracytoplasmic sperm injection failure include the absence of sperm, immotile sperm, immature sperm, abnormally structured sperm, and sperm with nuclear damage. Our knockout and knock-in mice models demonstrated that SEPTIN12 (SEPT12) is vital for the formation of sperm morphological characteristics during spermiogenesis. In the clinical aspect, mutated SEPT12 in men results in oligozoospermia or teratozoospermia or both...
November 16, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27835637/transcriptomic-variation-during-spermiogenesis-in-mouse-germ-cells
#6
Haiyang Zuo, Junfang Zhang, Liuguang Zhang, Xiaoxia Ren, Xiaoli Chen, Haisheng Hao, Xueming Zhao, Dong Wang
To explore variations in the transcription activity during spermiogenesis, round and elongated spermatids were collected from ICR/CD1 model mice using laser capture microdissection (LCM) and cauda epididymal sperm samples. The transcripts were sequenced using RNA-seq, and the reads were mapped to mm9. The majority of the reads (70%) in the round and elongated spermatids were mappable to known and predicted exons, but that in sperm was only 9%. The results of the distribution of reads suggested that alternative splicing was more complicated in sperm than in round and elongated spermatids...
2016: PloS One
https://www.readbyqxmd.com/read/27831554/the-control-of-male-fertility-by-spermatid-specific-factors-searching-for-contraceptive-targets-from-spermatozoon-s-head-to-tail
#7
REVIEW
Su-Ren Chen, Aalia Batool, Yu-Qian Wang, Xiao-Xia Hao, Chawn-Shang Chang, C Yan Cheng, Yi-Xun Liu
Male infertility due to abnormal spermatozoa has been reported in both animals and humans, but its pathogenic causes, including genetic abnormalities, remain largely unknown. On the other hand, contraceptive options for men are limited, and a specific, reversible and safe method of male contraception has been a long-standing quest in medicine. Some progress has recently been made in exploring the effects of spermatid-specifical genetic factors in controlling male fertility. A comprehensive search of PubMed for articles and reviews published in English before July 2016 was carried out using the search terms 'spermiogenesis failure', 'globozoospermia', 'spermatid-specific', 'acrosome', 'infertile', 'manchette', 'sperm connecting piece', 'sperm annulus', 'sperm ADAMs', 'flagellar abnormalities', 'sperm motility loss', 'sperm ion exchanger' and 'contraceptive targets'...
November 10, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27810970/the-drosophila-chromosomal-protein-mst77f-is-processed-to-generate-an-essential-component-of-mature-sperm-chromatin
#8
Shuhei Kimura, Benjamin Loppin
In most animals, the bulk of sperm DNA is packaged with sperm nuclear basic proteins (SNBPs), a diverse group of highly basic chromosomal proteins notably comprising mammalian protamines. The replacement of histones with SNBPs during spermiogenesis allows sperm DNA to reach an extreme level of compaction, but little is known about how SNBPs actually function in vivo Mst77F is a Drosophila SNBP with unique DNA condensation properties in vitro, but its role during spermiogenesis remains unclear. Here, we show that Mst77F is required for the compaction of sperm DNA and the production of mature sperm, through its cooperation with protamine-like proteins Mst35Ba/b...
November 2016: Open Biology
https://www.readbyqxmd.com/read/27803675/subcellular-evidence-for-biogenesis-of-autophagosomal-membrane-during-spermiogenesis-in-vivo
#9
Yufei Huang, Ping Yang, Tengfei Liu, Hong Chen, Xiaoya Chu, Nisar Ahmad, Qian Zhang, Quanfu Li, Lisi Hu, Yi Liu, Qiusheng Chen
Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27742209/knockdown-of-dynamitin-in-testes-significantly-decreased-male-fertility-in-drosophila-melanogaster
#10
Chun-Hong Wu, Qiong Zong, An-Li Du, Wei Zhang, Han-Chao Yao, Xiao-Qiang Yu, Yu-Feng Wang
Dynamitin (Dmn) is a major component of dynactin, a multiprotein complex playing important roles in a variety of intracellular motile events. We previously found that Wolbachia bacterial infection resulted in a reduction of Dmn protein. As Wolbachia may modify sperm in male hosts, we speculate that Dmn may have a function in male fertility. Here we used nosGal4 to drive Dmn knock down in testes of Drosophila melanogaster to investigate the functions of Dmn in spermatogenesis. We found that knockdown of Dmn in testes dramatically decreased male fertility, overexpression of Dmn in Wolbachia-infected males significantly rescued male fertility, indicating an important role of Dmn in inducing male fertility defects following Wolbachia infection...
December 1, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27717557/expression-of-katanin-p80-in-human-spermatogenesis
#11
Christiane Pleuger, Daniela Fietz, Katja Hartmann, Wolfgang Weidner, Sabine Kliesch, Moira K O'Bryan, Adriaan Dorresteijn, Martin Bergmann
OBJECTIVE: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. DESIGN: Gene expression analysis, histologic and immunohistochemical evaluation. SETTING: University research laboratories and andrological clinic. PATIENT(S): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27698683/dna-methylation-in-spermatogenesis-and-male-infertility
#12
Xiangrong Cui, Xuan Jing, Xueqing Wu, Meiqin Yan, Qiang Li, Yan Shen, Zhenqiang Wang
Infertility is a significant problem for human reproduction, with males and females equally affected. However, the molecular mechanisms underlying male infertility remain unclear. Spermatogenesis is a highly complex process involving mitotic cell division, meiosis cell division and spermiogenesis; during this period, unique and extensive chromatin and epigenetic modifications occur to bring about specific epigenetic profiles in spermatozoa. It has recently been suggested that the dysregulation of epigenetic modifications, in particular the methylation of sperm genomic DNA, may serve an important role in the development of numerous diseases...
October 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27682589/intraflagellar-transport-protein-ift20-is-essential-for-male-fertility-and-spermiogenesis-in-mice
#13
Zhengang Zhang, Wei Li, Yong Zhang, Ling Zhang, Maria E Teves, Hong Liu, Jerome F Strauss, Gregory J Pazour, James A Foster, Rex A Hess, Zhibing Zhang
Intraflagellar transport (IFT) is a conserved mechanism thought to be essential for the assembly and maintenance of cilia and flagella. However, little is known about its role in mammalian sperm flagella formation. To fill this gap, we disrupted the Ift20 gene in male germ cells. Homozygous mutant mice were infertile with significantly reduced sperm counts and motility. In addition, abnormally shaped elongating spermatid heads and bulbous round spermatids were found in the lumen of the seminiferous tubules...
September 28, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27682184/tracing-of-zinc-and-iron-in-experimentally-induced-varicocele-correlation-with-oxidative-nitrosative-and-carbonyl-stress
#14
S Gholirad, M Razi, H Hassani Bafrani
This study was designed to evaluate how varicocele (VCL) can time dependently induce zinc (Zn) and iron (Fe) toxicity in testicular tissue and to analyse the relation between heavy metals toxicity and lipid peroxidation, sperm DNA damage, nitrosative and carbonyl stresses. Twenty-four mature male Wistar rats were divided into control-sham and test groups, which were then submitted to experimentally induced VCL. Non-VCL-induced rats were considered as control-sham. The test groups were subdivided into three groups based on the sample collecting date (2, 6 and 8 months after VCL induction)...
September 29, 2016: Andrologia
https://www.readbyqxmd.com/read/27679827/antioxidant-and-protective-effects-of-royal-jelly-on-histopathological-changes-in-testis-of-diabetic-rats
#15
Elham Ghanbari, Vahid Nejati, Mozafar Khazaei
BACKGROUND: Diabetes is the most common endocrine disease. It has adverse effects on male reproductive function. Royal Jelly (RJ) has antioxidant and anti-diabetic effects and show protective effects against diabetes. OBJECTIVE: This study was conducted to evaluate the effect of RJ on histopathological alterations of the testicular tissue in streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS: In this experimental study, 28 adult Wistar rats were randomly divided into control (C), royal jelly (R), diabetic (D) and RJ-treated diabetic (D+R) groups...
August 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27677211/targeted-deletion-of-the-kv6-4-subunit-causes-male-sterility-due-to-disturbed-spermiogenesis
#16
Glenn Regnier, Elke Bocksteins, Waleed F Marei, Isabel Pintelon, Jean-Pierre Timmermans, Jo L M R Leroy, Dirk J Snyders
Electrically silent voltage-gated potassium (KvS) channel subunits (i.e. Kv5-Kv6 and Kv8-Kv9) do not form functional homotetrameric Kv channels, but co-assemble with Kv2 subunits, generating functional heterotetrameric Kv2--KvS channel complexes in which the KvS subunits modulate the Kv2 channel properties. Several KvS subunits are expressed in testis tissue but knowledge about their contribution to testis physiology is lacking. Here, we report that the targeted deletion of Kv6.4 in a transgenic mouse model (Kcng4-/-) causes male sterility as offspring from homozygous females were only obtained after mating with wild-type (WT) or heterozygous males...
September 28, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27649891/low-levels-of-prss37-protein-in-sperm-are-associated-with-many-cases-of-unexplained-male-infertility
#17
Jianbing Liu, Chunling Shen, Weimin Fan, Yan Chen, Aijun Zhang, Yun Feng, Zheng Li, Ying Kuang, Zhugang Wang
PRSS37, a putative trypsin-like serine protease, is highly conserved during mammalian evolution as revealed by multiple sequence alignment. Mice deficient for Prss37 gene exhibit male infertility, but their mating behavior, spermatogenesis, sperm morphology, and motility remain unaffected, similar to a situation called unexplained male infertility (UMI) in men (human being). Here, we demonstrated that PRSS37 is restrictively expressed in human testis, where it is mainly located in the elongating and elongated spermatids during spermiogenesis as shown by immunohistochemical analysis of normal human testicular sections...
September 20, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27647534/adenylation-by-testis-specific-cytoplasmic-poly-a-polymerase-papolb-tpap-is-essential-for-spermatogenesis
#18
Shin-Ichi Kashiwabara, Satsuki Tsuruta, Keitaro Okada, Yutaro Yamaoka, Tadashi Baba
The testis-specific cytoplasmic poly(A) polymerase PAPOLB/TPAP is essential for spermatogenesis. Although this enzyme is responsible for poly(A) tail extension of a subset of mRNAs in round spermatids, the stability and translational efficiency of these mRNAs are unaffected by the absence of PAPOLB. To clarify the functional importance of this enzyme's adenylation activity, we produced PAPOLB-null mice expressing a polyadenylation-defective PAPOLB mutant (PAPOLB(D114A)), in which the catalytic Asp at residue 114 was mutated to Ala...
September 18, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27561408/a-novel-soxb2-gene-is-required-for-maturation-of-sperm-nucleus-during-spermiogenesis-in-the-chinese-mitten-crab-eriocheir-sinensis
#19
Zhi-Qiang Liu, Xue-Hui Jiang, Hai-Yan Qi, Liang-Wei Xiong, Gao-Feng Qiu
SRY-related HMG box (Sox) genes are characterized by the presence of a DNA-binding HMG domain and involved in a diverse range of developmental processes. In this study, we identified a novel Sox gene, designated as EsSoxB2-1, from the Chinese mitten crab Eriocheir sinensis. The EsSoxB2-1 encodes a protein of 259 amino acids, sharing the highest identity with the beetle Tribolium castaneum SOX21b. Unlike insect Sox21b, however, EsSoxB2-1 is intronless and exhibits a gonad-specific expression pattern at both mRNA and protein level...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27512614/genomic-and-expression-analysis-of-transition-proteins-in-drosophila
#20
Zain A Alvi, Tin-Chun Chu, Valerie Schawaroch, Angela V Klaus
The current study was aimed at analyzing putative protein sequences of the transition protein-like proteins in 12 Drosophila species based on the reference sequences of transition protein-like protein (Tpl (94D) ) expressed in Drosophila melanogaster sperm nuclei. Transition proteins aid in transforming chromatin from a histone-based nucleosome structure to a protamine-based structure during spermiogenesis - the post-meiotic stage of spermatogenesis. Sequences were obtained from NCBI Ref-Seq database using NCBI ORF-Finder (PSI-BLAST)...
September 2015: Spermatogenesis
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