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Spermiogenesis

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https://www.readbyqxmd.com/read/29907856/deletion-of-ddb1-and-cul4-associated-factor-17-dcaf17-gene-causes-spermatogenesis-defects-and-male-infertility-in-mice
#1
Asmaa Ali, Bhavesh V Mistry, Hala A Ahmed, Razan Abdulla, Hassan A Amer, Abdelbary Prince, Anas M Alazami, Fowzan S Alkuraya, Abdullah Assiri
DDB1- and CUL4-associated factor 17 (Dcaf17) is a member of DCAF family genes that encode substrate receptor proteins for Cullin-RING E3 ubiquitin ligases, which play critical roles in many cellular processes. To unravel the function of DCAF17, we performed expression profiling of Dcaf17 in different tissues of wild type mouse by qRT-PCR and generated Dcaf17 knockout mice by gene targeting. Expression profiling of Dcaf17 showed highest expression in testis. Analyses of Dcaf17 transcripts during post-natal development of testis at different ages displayed gradual increase in Dcaf17 mRNA levels with the age...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29882505/review-spermatogenesis-in-the-bull
#2
C Staub, L Johnson
Spermatogenesis is a finely regulated process of germ cell multiplication and differentiation leading to the production of spermatozoa in the seminiferous tubules. Spermatogenesis can be divided into three parts: spermatocytogenesis, meiosis and spermiogenesis. During spermatocytogenesis, germ cells engage in a cycle of several mitotic divisions that increases the yield of spermatogenesis and to renew stem cells and produce spermatogonia and primary spermatocytes. Meiosis involves duplication and exchange of genetic material and two cell divisions that reduce the chromosome number and yield four haploid round spermatids...
June 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29866902/sox30-initiates-transcription-of-haploid-genes-during-late-meiosis-and-spermiogenesis-in-mouse-testes
#3
Shun Bai, Kaiqiang Fu, Huiqi Yin, Yiqiang Cui, Qiuling Yue, Wenbo Li, Le Cheng, Huanhuan Tan, Xiaofei Liu, Yueshuai Guo, Yingwen Zhang, Jie Xie, Wenxiu He, Yuanyuan Wang, Hua Feng, Changpeng Xin, Jinwen Zhang, Mingyan Lin, Bin Shen, Zheng Sun, Xuejiang Guo, Ke Zheng, Lan Ye
Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators of progenitor cell fate. Here, we report that expression of Sox30 is restricted to meiotic spermatocytes and postmeiotic haploids. Sox30 mutant males are sterile due to spermiogenic arrest at the early round spermatid stage. Specifically, in the absence of Sox30, proacrosomic vesicles fail to form a single acrosomal organelle, and spermatids arrest at step 2-3. Although most Sox30 mutant spermatocytes progress through meiosis, accumulation of diplotene spermatocytes indicates a delayed or impaired transition from meiotic to postmeiotic stages...
June 4, 2018: Development
https://www.readbyqxmd.com/read/29851651/roles-of-three-es-caspases-during-spermatogenesis-and-cadmium-induced-apoptosis-in-eriocheir-sinensis
#4
Ya-Ru Xu, Wan-Xi Yang
Functions of Caspases remain obscure in Crustacea. We studied the existence and participations of apoptosis-related factors in Eriocheir sinensis testis. Three Es-Caspases (Es-Caspase 3/ 7/ 8) in E. sinensis were cloned and characterized. We observed that three es-caspases mRNA had specific expression patterns during spermiogenesis, with weak signal around the nucleus and invaginated acrosomal vesicle in early-stage spermatids, became stronger in middle-stage, finally focused on the acrosomal tube and nucleus in mature sperm...
May 24, 2018: Aging
https://www.readbyqxmd.com/read/29848638/the-transcription-factor-sox30-is-a-key-regulator-of-mouse-spermiogenesis
#5
Daoqin Zhang, Dan Xie, Xiwen Lin, Longfei Ma, Jian Chen, Daoqi Zhang, Yang Wang, Shuguang Duo, Yanmin Feng, Chunwei Zheng, Binjie Jiang, Yan Ning, Chunsheng Han
The postmeiotic development of male germ cells, also known as spermiogenesis, features the coordinated expression of a large number of spermatid-specific genes. However, only a limited number of key transcription factors have been identified and the underlying regulatory mechanisms remain largely unknown. Here, we report that SOX30, the most-divergent member of the Sry-related high-motility group box (SOX) family of transcription factors, is essential for mouse spermiogenesis. The SOX30 protein was predominantly expressed in spermatids, while its transcription was regulated by retinoic acid and by MYBL1 before and during meiosis...
May 30, 2018: Development
https://www.readbyqxmd.com/read/29782059/testicular-histomorphometry-and-the-proliferative-and-apoptotic-activities-of-the-seminiferous-epithelium-in-syrian-hamster-during-spontaneous-recrudescence-after-exposure-to-short-photoperiod
#6
Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Concepción Ferrer, Manuel Canteras, María Del Mar Sánchez-Huertas, Luis Miguel Pastor
Syrian hamsters are photoperiodic rodents in which reproduction, including testicular function, is stimulated by long photoperiod exposure and curtailed by exposure to a short photoperiod. The objectives of this study were to characterize the testis histomorphometrically and to determine the role of the proliferation and apoptosis phenomena in the recovery of the seminiferous epithelium during spontaneous recrudescence after exposure to short photoperiod. The study was performed using conventional light microscopy, proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end labelling staining, image analysis software, and transmission electron microscopy in three recrudescence groups: initial recrudescence (IR), advanced recrudescence (AR) and total recrudescence (TR)...
May 21, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29780056/potential-roles-of-the-poly-a-binding-proteins-in-translational-regulation-during-spermatogenesis
#7
Saffet Ozturk, Fatma Uysal
Spermatogenesis is briefly defined as the production of mature spermatozoa from spermatogonial stem cells at the end of a strictly regulated process. It is well known that, to a large extent, transcriptional activity ceases at mid-spermiogenesis. Several mRNAs transcribed during early stages of spermatogenesis are stored as ribonucleoproteins (RNPs). During the later stages, translational control of these mRNAs is mainly carried out in a time dependent-manner by poly(A)-binding proteins (PABPs) in cooperation with other RNA-binding proteins and translation-related factors...
May 18, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29761655/fennel-induces-cytotoxic-effects-against-testicular-germ-cells-in-mice-evidences-for-suppressed-pre-implantation-embryo-development
#8
Aram Minas, Gholamreza Najafi, Ali Shalizar Jalali, Mazdak Razi
Foeniculum vulgare (FVE; fennel) is an aromatic plant belonging to Umbelliferae family, which is widely used in traditional societies because of its different pharmaceutical properties. To uncover the fennel-derived essential oil (FVEO)-induced effects on male reproductive potential, 24 mature male albino mice were divided into, control, 0.37, 0.75, and 1.5 mg kg-1 FVEO-received groups. Following 35 days, the animals were euthanized and the testicular tissue and sperm samples were collected. The histological alterations, tubular differentiation (TDI), spermiogenesis (SPI) indices, apoptosis ratio, and RNA damage of germinal cells were analyzed...
May 15, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29759112/remodelling-of-mitochondria-during-spermiogenesis-of-chinese-soft-shelled-turtle-pelodiscus-sinensis
#9
Abdul Haseeb, Hong Chen, Yufei Huang, Ping Yang, Xuejing Sun, Adeela Iqbal, Nisar Ahmed, Taozhi Wang, Noor Samad Gandahi, Xuebing Bai, Qiusheng Chen
Mitochondria are vital cellular organelles that have the ability to change their shape under different conditions, such as in response to stress, disease, changes in metabolic rate, energy requirements and apoptosis. In the present study, we observed remodelling of mitochondria during spermiogenesis and its relationship with mitochondria-associated granules (MAG). At the beginning of spermiogenesis, mitochondria are characterised by their round shape. As spermiogenesis progresses, the round-shaped mitochondria change into elongated and then swollen mitochondria, subsequently forming a crescent-like shape and finally developing into onion-like shaped mitochondria...
May 15, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29739818/spermiogenesis-and-male-fertility-require-the-function-of-suppressor-of-hairy-wing-in-somatic-cyst-cells-of-drosophila
#10
Tingting Duan, Pamela K Geyer
Drosophila Suppressor of Hairy-wing [Su(Hw)] protein is an example of a multivalent transcription factor. Although best known for its role in establishing the chromatin insulator of the gypsy retrotransposon, Su(Hw) functions as an activator and repressor at non- gypsy genomic sites. It remains unclear how the different regulatory activities of Su(Hw) are utilized during development. Motivated from observations of spatially restricted expression of Su(Hw) in the testis, we investigated the role of Su(Hw) in spermatogenesis to advance an understanding of its developmental contributions as an insulator, repressor and activator protein...
May 8, 2018: Genetics
https://www.readbyqxmd.com/read/29724783/-elovl4-and-fa2h-expression-during-rat-spermatogenesis-a-link-to-the-very-long-chain-pufa-typical-of-germ-cell-sphingolipids
#11
Florencia X Santiago Valtierra, Daniel A Penalva, Jessica M Luquez, Natalia E Furland, Claudia Vazquez, Juan G Reyes, Marta I Aveldano, Gerardo M Oresti
The sphingolipids (SL) of rodent spermatogenic cells (spermatocytes, spermatids) and spermatozoa contain nonhydroxylated and 2-hydroxylated versions of very-long-chain (C26 to C32) PUFA (n-V and h-V, respectively), not present in Sertoli cells. Here we investigated the expression of selected fatty acid elongases ( Elovl ), with a focus on Elovl4 , and a fatty acid 2-hydroxylase ( Fa2h ), in rat testes with postnatal development and germ cell differentiation. Along with Elovl5 and Elovl2 , Elovl4 was actively transcribed in the adult testis...
May 3, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29716999/the-histone-methyltransferase-setd2-is-required-for-expression-of-acrosin-binding-protein-1and-protamines-and-essential-for-spermiogenesis-in-mice
#12
Xiaoli Zuo, Bowen Rong, Li Li, Ruitu Lv, Fei Lan, Ming-Han Tong
Spermatogenesis is precisely controlled by complex gene expression programs and involves epigenetic reprogramming including histone modification and DNA methylation. SET domain-containing 2 (Setd2) is the predominant histone methyltransferase catalyzing the trimethylation of histone H3 Lysine 36 (H3K36me3) and plays key roles in embryonic stem cell differentiation and somatic cell development. However, its role in male germ cell development remains elusive. Here we demonstrate an essential role of Setd2for spermiogenesis,the final stage of spermatogenesis...
May 1, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29705697/a-monolayer-microfluidic-device-supporting-mouse-spermatogenesis-with-improved-visibility
#13
Hiroyuki Yamanaka, Mitsuru Komeya, Hiroko Nakamura, Hiroyuki Sanjo, Takuya Sato, Masahiro Yao, Hiroshi Kimura, Teruo Fujii, Takehiko Ogawa
In our previous study, we produced a microfluidic device (MFD) which successfully maintained spermatogenesis for over 6 months in mouse testis tissues loaded in the device. In the present study, we developed a new MFD, a monolayer device (ML-D) with a barrier structure consisting of pillars and slits, which is simpler in design and easier to make. This ML-D was also effective for inducing mouse spermatogenesis and maintained it for a longer period than the conventional culture method. In addition, we devised a way of introducing sample tissue into the device during its production, just before bonding the upper layer of polydimethylsiloxane (PDMS) and bottom glass slide...
June 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29705659/effect-of-hcg-and-ovaprim%C3%A2-on-reproductive-characteristics-of-male-levantine-scraper-capoeta-damascina-valenciennes-1842
#14
Vahid Zadmajid, Somayeh Bashiri, Nastaran Sharafi, Ian Anthony Ernest Butts
Species richness and abundance within the genus Capoeta has been depleted. As such, there is great need for developing assisted reproductive technologies for controlling reproduction in captivity. Here, we conducted in vivo studies with single administrations of human chorionic gonadotropin (hCG) and Ovaprim™ [(D-Arg6 , Pro9 NEt)-sGnRH + domperidone] in wild-caught Levantine scraper, Capoeta damascina and then evaluated milt characteristics, fertilization success, serum sex steroids, and spermatogenesis via histological testicular development...
July 15, 2018: Theriogenology
https://www.readbyqxmd.com/read/29701755/domain-functional-analyses-of-piwil1-and-pabpc1-indicate-their-synergistic-roles-in-protein-translation-via-3-utrs-of-meiotic-mrnas
#15
Yaoyao Wu, Kaibiao Xu, Huayu Qi
Translational regulation plays a central role during post-meiotic development of male germ cells. Previous studies suggested that PIWIL1, a PIWI-interacting RNA (piRNA) binding protein that is critical for sperm development, participates in the maintenance and translational regulation of post-meiotic mRNAs in haploid spermatids. However, how PIWIL1 regulates protein translation remains largely unclear. Using biochemical assays, we show here that PIWIL1 utilizes different domains to interact with post-meiotic mRNAs and PABPC1, a general protein translation regulator...
April 25, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29663597/morphological-and-histological-structure-of-the-male-reproductive-system-of-the-water-strider-gerris-lacustris-linnaeus-1758-gerridae-heteroptera
#16
Selami Candan, Nurcan Özyurt Koçakoğlu, Zekiye Suludere
This work presents the male reproductive system morphology and histology of the water strider Gerris lacustris (Linnaeus 1758) (Gerridae, Heteroptera) using light microscopy and scanning electron microscopy. The male reproductive system of G. lacustris comprise of a pair of testes, two vasa deferentia, two seminal vesicles, an ejaculatory duct. There is no bulbus ejaculatorius and the long vas deferantia uniting to form a simple ductus ejaculatorius which is connected to the aedeagus. The testes are white colored and this cylindiric-shaped structure lies along genital abdominal segment...
April 16, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29659998/the-arginine-methyltransferase-carm1-represses-p300%C3%A2-act%C3%A2-crem%C3%AF-activity-and-is-required-for-spermiogenesis
#17
Jianqiang Bao, Sophie Rousseaux, Jianjun Shen, Kevin Lin, Yue Lu, Mark T Bedford
CARM1 is a protein arginine methyltransferase (PRMT) that has been firmly implicated in transcriptional regulation. However, the molecular mechanisms by which CARM1 orchestrates transcriptional regulation are not fully understood, especially in a tissue-specific context. We found that Carm1 is highly expressed in the mouse testis and localizes to the nucleus in spermatids, suggesting an important role for Carm1 in spermiogenesis. Using a germline-specific conditional Carm1 knockout mouse model, we found that it is essential for the late stages of haploid germ cell development...
May 18, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29657074/nucleotide-variability-of-protamine-genes-influencing-bull-sperm-motility-variables
#18
Yathish H M, Subodh Kumar, Rajni Chaudhary, Chinmoy Mishra, Sivakumar A, Amit Kumar, Anuj Chauhan, S K Ghosh, Abhijit Mitra
Protamines (PRMs), important proteins of chromatin condensation in spermiogenesis, are promising candidate genes to explore markers of sperm motility. The coding and in-silico predicted promoter regions of these genes were investigated in 102 crossbred and 32 purebred cattle. Also, mRNA quantification was done to explore its possibility as diagnostic tool of infertility. The PCR-SSCP analysis indicated there were two band patterns only in fragment I of the PRM1 and fragment II of the PRM2 gene. The sequence analysis revealed A152G and G179A transitions in the PRM1 gene...
June 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29653258/cadmium-inhibits-lysine-acetylation-and-succinylation-inducing-testicular-injury-of-mouse-during-development
#19
Qiangzhen Yang, Peifei Li, Yi Wen, Sisi Li, Jun Chen, Xurui Liu, Lirui Wang, Xinhong Li
The toxic effects of cadmium (Cd) in the reproductive system have been confirmed, and lysine acetylation and succinylation play important roles in spermatogenesis. However, little attention determined whether Cd could affect lysine acylation and how it might have an impact on the reproductive system. Therefore, with the goal of contributing to this subject, we have examined the effects of Cd on lysine acetylation and succinylation of proteins in the germ cells of male mice testes during different developmental stages...
July 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29609617/genome-wide-analysis-of-gene-regulation-mechanisms-during-drosophila-spermatogenesis
#20
Petr P Laktionov, Daniil A Maksimov, Stanislav E Romanov, Polina A Antoshina, Olga V Posukh, Helen White-Cooper, Dmitry E Koryakov, Stepan N Belyakin
BACKGROUND: During Drosophila spermatogenesis, testis-specific meiotic arrest complex (tMAC) and testis-specific TBP-associated factors (tTAF) contribute to activation of hundreds of genes required for meiosis and spermiogenesis. Intriguingly, tMAC is paralogous to the broadly expressed complex Myb-MuvB (MMB)/dREAM and Mip40 protein is shared by both complexes. tMAC acts as a gene activator in spermatocytes, while MMB/dREAM was shown to repress gene activity in many cell types. RESULTS: Our study addresses the intricate interplay between tMAC, tTAF, and MMB/dREAM during spermatogenesis...
April 2, 2018: Epigenetics & Chromatin
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