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Chun-Hong Wu, Qiong Zong, An-Li Du, Wei Zhang, Han-Chao Yao, Xiao-Qiang Yu, Yu-Feng Wang
Dynamitin (Dmn) is a major component of dynactin, a multiprotein complex playing important roles in a variety of intracellular motile events. We previously found that Wolbachia bacterial infection resulted in a reduction of Dmn protein. As Wolbachia may modify sperm in male hosts, we speculate that Dmn may have a function in male fertility. Here we used nosGal4 to drive Dmn knock down in testes of Drosophila melanogaster to investigate the functions of Dmn in spermatogenesis. We found that knockdown of Dmn in testes dramatically decreased male fertility, overexpression of Dmn in Wolbachia-infected males significantly rescued male fertility, indicating an important role of Dmn in inducing male fertility defects following Wolbachia infection...
October 11, 2016: Developmental Biology
Christiane Pleuger, Daniela Fietz, Katja Hartmann, Wolfgang Weidner, Sabine Kliesch, Moira K O'Bryan, Adriaan Dorresteijn, Martin Bergmann
OBJECTIVE: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. DESIGN: Gene expression analysis, histologic and immunohistochemical evaluation. SETTING: University research laboratories and andrological clinic. PATIENT(S): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome...
October 4, 2016: Fertility and Sterility
Xiangrong Cui, Xuan Jing, Xueqing Wu, Meiqin Yan, Qiang Li, Yan Shen, Zhenqiang Wang
Infertility is a significant problem for human reproduction, with males and females equally affected. However, the molecular mechanisms underlying male infertility remain unclear. Spermatogenesis is a highly complex process involving mitotic cell division, meiosis cell division and spermiogenesis; during this period, unique and extensive chromatin and epigenetic modifications occur to bring about specific epigenetic profiles in spermatozoa. It has recently been suggested that the dysregulation of epigenetic modifications, in particular the methylation of sperm genomic DNA, may serve an important role in the development of numerous diseases...
October 2016: Experimental and Therapeutic Medicine
Zhengang Zhang, Wei Li, Yong Zhang, Ling Zhang, Maria E Teves, Hong Liu, Jerome F Strauss, Gregory J Pazour, James A Foster, Rex A Hess, Zhibing Zhang
Intraflagellar transport (IFT) is a conserved mechanism thought to be essential for the assembly and maintenance of cilia and flagella. However, little is known about its role in mammalian sperm flagella formation. To fill this gap, we disrupted the Ift20 gene in male germ cells. Homozygous mutant mice were infertile with significantly reduced sperm counts and motility. In addition, abnormally shaped elongating spermatid heads and bulbous round spermatids were found in the lumen of the seminiferous tubules...
September 28, 2016: Molecular Biology of the Cell
S Gholirad, M Razi, H Hassani Bafrani
This study was designed to evaluate how varicocele (VCL) can time dependently induce zinc (Zn) and iron (Fe) toxicity in testicular tissue and to analyse the relation between heavy metals toxicity and lipid peroxidation, sperm DNA damage, nitrosative and carbonyl stresses. Twenty-four mature male Wistar rats were divided into control-sham and test groups, which were then submitted to experimentally induced VCL. Non-VCL-induced rats were considered as control-sham. The test groups were subdivided into three groups based on the sample collecting date (2, 6 and 8 months after VCL induction)...
September 29, 2016: Andrologia
Elham Ghanbari, Vahid Nejati, Mozafar Khazaei
BACKGROUND: Diabetes is the most common endocrine disease. It has adverse effects on male reproductive function. Royal Jelly (RJ) has antioxidant and anti-diabetic effects and show protective effects against diabetes. OBJECTIVE: This study was conducted to evaluate the effect of RJ on histopathological alterations of the testicular tissue in streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS: In this experimental study, 28 adult Wistar rats were randomly divided into control (C), royal jelly (R), diabetic (D) and RJ-treated diabetic (D+R) groups...
August 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
Glenn Regnier, Elke Bocksteins, Waleed F Marei, Isabel Pintelon, Jean-Pierre Timmermans, Jo L M R Leroy, Dirk J Snyders
Electrically silent voltage-gated potassium (KvS) channel subunits (i.e. Kv5-Kv6 and Kv8-Kv9) do not form functional homotetrameric Kv channels, but co-assemble with Kv2 subunits, generating functional heterotetrameric Kv2--KvS channel complexes in which the KvS subunits modulate the Kv2 channel properties. Several KvS subunits are expressed in testis tissue but knowledge about their contribution to testis physiology is lacking. Here, we report that the targeted deletion of Kv6.4 in a transgenic mouse model (Kcng4-/-) causes male sterility as offspring from homozygous females were only obtained after mating with wild-type (WT) or heterozygous males...
September 28, 2016: Reproduction, Fertility, and Development
Jianbing Liu, Chunling Shen, Weimin Fan, Yan Chen, Aijun Zhang, Yun Feng, Zheng Li, Ying Kuang, Zhugang Wang
PRSS37, a putative trypsin-like serine protease, is highly conserved during mammalian evolution as revealed by multiple sequence alignment. Mice deficient for Prss37 gene exhibit male infertility, but their mating behavior, spermatogenesis, sperm morphology, and motility remain unaffected, similar to a situation called unexplained male infertility (UMI) in men (human being). Here, we demonstrated that PRSS37 is restrictively expressed in human testis, where it is mainly located in the elongating and elongated spermatids during spermiogenesis as shown by immunohistochemical analysis of normal human testicular sections...
September 20, 2016: Acta Biochimica et Biophysica Sinica
Shin-Ichi Kashiwabara, Satsuki Tsuruta, Keitaro Okada, Yutaro Yamaoka, Tadashi Baba
The testis-specific cytoplasmic poly(A) polymerase PAPOLB/TPAP is essential for spermatogenesis. Although this enzyme is responsible for poly(A) tail extension of a subset of mRNAs in round spermatids, the stability and translational efficiency of these mRNAs are unaffected by the absence of PAPOLB. To clarify the functional importance of this enzyme's adenylation activity, we produced PAPOLB-null mice expressing a polyadenylation-defective PAPOLB mutant (PAPOLB(D114A)), in which the catalytic Asp at residue 114 was mutated to Ala...
September 18, 2016: Journal of Reproduction and Development
Zhi-Qiang Liu, Xue-Hui Jiang, Hai-Yan Qi, Liang-Wei Xiong, Gao-Feng Qiu
SRY-related HMG box (Sox) genes are characterized by the presence of a DNA-binding HMG domain and involved in a diverse range of developmental processes. In this study, we identified a novel Sox gene, designated as EsSoxB2-1, from the Chinese mitten crab Eriocheir sinensis. The EsSoxB2-1 encodes a protein of 259 amino acids, sharing the highest identity with the beetle Tribolium castaneum SOX21b. Unlike insect Sox21b, however, EsSoxB2-1 is intronless and exhibits a gonad-specific expression pattern at both mRNA and protein level...
2016: Scientific Reports
Zain A Alvi, Tin-Chun Chu, Valerie Schawaroch, Angela V Klaus
The current study was aimed at analyzing putative protein sequences of the transition protein-like proteins in 12 Drosophila species based on the reference sequences of transition protein-like protein (Tpl (94D) ) expressed in Drosophila melanogaster sperm nuclei. Transition proteins aid in transforming chromatin from a histone-based nucleosome structure to a protamine-based structure during spermiogenesis - the post-meiotic stage of spermatogenesis. Sequences were obtained from NCBI Ref-Seq database using NCBI ORF-Finder (PSI-BLAST)...
September 2015: Spermatogenesis
Rafael Henrique Nóbrega, Lázaro Wender Oliveira de Jesus, Renato Massaaki Honji, Maria Inês Borella
This work describes gonadotropic (GtH) cells and their morphological and immunohistochemical changes during the spermatogenic cycle of Serrasalmus maculatus (continuous spermatogenesis) and Pimelodus maculatus (seasonal spermatogenesis). GtH cells, widely distributed in the proximal pars distalis of the adenohypophysis, were characterized as round-shaped cells with eccentric nucleus, and cytoplasm with basophilic secretory granules and a variable number of vacuoles for both species. Immunohistochemistry against β-follicle-stimulating hormone (Fsh) and β-luteinizing hormone (Lh) in adjacent sections showed two separated GtH-producing cell populations, and a third population where both GtHs are expressed in the same cell for both species...
August 5, 2016: Fish Physiology and Biochemistry
Luigi Rosati, Marisa Agnese, Maria Maddalena Di Fiore, Piero Andreuccetti, Marina Prisco
P450 aromatase is a key enzyme in steroidogenesis involved in the conversion of testosterone into 17β-estradiol. We investigated the localization and the expression of P450 aromatase in Podarcis sicula testes during the different phases of the reproductive cycle: summer stasis (July-August), early autumnal resumption (September), middle autumnal resumption (October-November), winter stasis (December-February), spring resumption (March-April) and the reproductive period (May-June). Using immunohistochemistry, we demonstrated that the P450 aromatase is always present in somatic and germ cells of P...
August 1, 2016: Journal of Experimental Biology
Rafael M C Melo, Yves M Ribeiro, Ronald K Luz, Nilo Bazzoli, Elizete Rizzo
Understanding the influence of different temperature conditions on the spermatogenesis is important for improvement of the fish aquaculture. This study evaluated the influence of low temperature on structural and quantitative dynamics of the spermatogenesis in Oreochromis niloticus. Adult males were cultivated with room temperature water (20.28-22.46°C) and testes were collected for histological, ultrastructural and morphometric analyses. This species has unrestricted lobular testis with cystic spermatogenesis and type I spermiogenesis that results in a single anacrosomal aquasperm...
September 2016: Animal Reproduction Science
Chieh-Ying Wang, Ming-Chu Tang, Wen-Chi Chang, Kenryo Furushima, Chuan-Wei Jang, Richard R Behringer, Chun-Ming Chen
Bobby sox homolog (Bbx) is an evolutionally conserved gene, but its biological function remains elusive. Here, we characterized defects of Bbx mutant rats that were created by PiggyBac-mediated insertional mutagenesis. Smaller body size and male infertility were the two major phenotypes of homozygous Bbx mutants. Bbx expression profile analysis showed that Bbx was more highly expressed in the testis and pituitary gland than in other organs. Histology and hormonal gene expression analysis of control and Bbx-null pituitary glands showed that loss of Bbx appeared to be dispensable for pituitary histogenesis and the expression of major hormones...
September 2016: Biology of Reproduction
Martina Trost, Ariane C Blattner, Stefano Leo, Christian F Lehner
The terminal differentiation of adult stem cell progeny depends on transcriptional control. A dramatic change in gene expression programs accompanies the transition from proliferating spermatogonia to postmitotic spermatocytes, which prepare for meiosis and subsequent spermiogenesis. More than a thousand spermatocyte-specific genes are transcriptionally activated in early Drosophila spermatocytes. Here we describe the identification and initial characterization of dany, a gene required in spermatocytes for the large-scale change in gene expression...
July 15, 2016: Development
Ning Song, Daisuke Endo, Bin Song, Yasuaki Shibata, Takehiko Koji
Mammalian spermatogenesis is a progressive process comprising spermatogonial proliferation, spermatocytic meiosis, and later spermiogenesis, which is considered to be under the regulation of epigenetic parameters. To gain insights into the significance of DNA methylation in early spermatogenesis, 5-azadC was used as a molecular biological tool to mimic the level of DNA methylation in vivo. Since the drug is incorporated into DNA during the S-phase, spermatogonia and spermatocytes would be affected primarily in mouse spermatogenesis...
June 15, 2016: Toxicology
Gilda Cobellis, Rosaria Meccariello, Rosanna Chianese, Teresa Chioccarelli, Silvia Fasano, Riccardo Pierantoni
Endocannabinoids control male reproduction acting at central and local level via cannabinoid receptors. The cannabinoid receptor CB1 has been characterized in the testis, in somatic and germ cells of mammalian and non-mammalian animal models, and its activity related to Leydig cell differentiation, steroidogenesis, spermiogenesis, sperm quality, and maturation. In this short review, we provide a summary of the insights concerning neuroendocrine CB1 activity in male reproduction focusing on adult Leydig cell ontogenesis and steroid biosynthesis...
2016: Frontiers in Endocrinology
Parvin Sabeti, Soheila Pourmasumi, Tahereh Rahiminia, Fatemeh Akyash, Ali Reza Talebi
Sperm is particularly susceptible to reactive oxygen species (ROS) during critical phases of spermiogenesis. However, the level of seminal ROS is restricted by seminal antioxidants which have beneficial effects on sperm parameters and developmental potentials. Mitochondria and sperm plasma membrane are two major sites of ROS generation in sperm cells. Besides, leukocytes including polymer phonuclear (PMN) leukocytes and macrophages produce broad category of molecules including oxygen free radicals, non-radical species and reactive nitrogen species...
April 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
Xiao-Ping Li, Chao-Liang Hao, Qian Wang, Xiao-Mei Yi, Zhi-Sheng Jiang
The present study investigated the H19 gene methylation status in male infertility. Between March 2013 and June 2014, semen samples were collected from 15 normal fertile males and 15 males experiencing infertility, and routine analysis and sperm morphological assessment were performed. The semen samples were subjected to density gradient centrifugation to separate the sperm fraction, and genomic DNA from the sperms was extracted and treated for bisulfite modification. Following in vitro amplification by polymerase chain reaction (PCR), the purified PCR products were cloned into pMD(®)18-T vectors and successful cloning was confirmed by restriction enzyme digestion...
July 2016: Experimental and Therapeutic Medicine
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