keyword
MENU ▼
Read by QxMD icon Read
search

Spermiogenesis

keyword
https://www.readbyqxmd.com/read/28086791/identification-of-functional-enolase-genes-of-the-silkworm-bombyx-mori-from-public-databases-with-a-combination-of-dry-and-wet-bench-processes
#1
Akira Kikuchi, Takeru Nakazato, Katsuhiko Ito, Yosui Nojima, Takeshi Yokoyama, Kikuo Iwabuchi, Hidemasa Bono, Atsushi Toyoda, Asao Fujiyama, Ryoichi Sato, Hiroko Tabunoki
BACKGROUND: Various insect species have been added to genomic databases over the years. Thus, researchers can easily obtain online genomic information on invertebrates and insects. However, many incorrectly annotated genes are included in these databases, which can prevent the correct interpretation of subsequent functional analyses. To address this problem, we used a combination of dry and wet bench processes to select functional genes from public databases. RESULTS: Enolase is an important glycolytic enzyme in all organisms...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28067790/rab10-interacts-with-the-male-germ-cell-specific-gtpase-activating-protein-during-mammalian-spermiogenesis
#2
Ying-Hung Lin, Chih-Chun Ke, Ya-Yun Wang, Mei-Feng Chen, Tsung-Ming Chen, Wei-Chi Ku, Han-Sun Chiang, Chung-Hsin Yeh
According to recent estimates, 2%-15% of couples are sterile, and approximately half of the infertility cases are attributed to male reproductive factors. However, the reasons remain undefined in approximately 25% of male infertility cases, and most infertility cases exhibit spermatogenic defects. Numerous genes involved in spermatogenesis still remain unknown. We previously identified Male Germ Cells Rab GTPase-Activating Proteins (MGCRABGAPs) through cDNA microarray analysis of human testicular tissues with spermatogenic defects...
January 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28030332/spermatological-characteristics-of-the-enigmatic-monogenean-dictyocotyle-coeliaca-nybelin-1941-monopisthocotylea-monocotylidae-reveal-possible-adaptation-to-endoparasitism
#3
Magdaléna Bruňanská, John S Mackiewicz, Larisa G Poddubnaya
Spermatological characters of Dictyocotyle coeliaca Nybelin, a unique endoparasitic monogenean from the body cavity of the ray Amblyraja radiata (Elasmobranchii: Rajidae), was investigated by means of scanning and transmission electron microscopy for the first time. The process of the spermatozoon formation begins with the appearance of the differentiation zone which contains two centrioles. Subsequently developed two free flagella rotate to lie parallel before their fusing. After fusion, both the mitochondrion and nucleus migrate alongside the axonemes...
March 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28008940/deficiency-of-mkrn2-causes-abnormal-spermiogenesis-and-spermiation-and-impairs-male-fertility
#4
Xu Qian, Lin Wang, Bo Zheng, Zhu-Mei Shi, Xin Ge, Cheng-Fei Jiang, Ying-Chen Qian, Dong-Mei Li, Wei Li, Xue Liu, Yu Yin, Ji-Tai Zheng, Hua Shen, Min Wang, Xue-Jiang Guo, Jun He, Marie Lin, Ling-Zhi Liu, Jia-Hao Sha, Bing-Hua Jiang
Although recent studies have shed insights on some of the potential causes of male infertility, new underlining molecular mechanisms still remain to be elucidated. Makorin-2 (Mkrn2) is an evolutionarily conserved gene whose biological functions are not fully known. We developed an Mrkn2 knockout mouse model to study the role of this gene, and found that deletion of Mkrn2 in mice led to male infertility. Mkrn2 knockout mice produced abnormal sperms characterized by low number, poor motility, and aberrant morphology...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28003215/sirt1-regulates-acrosome-biogenesis-by-modulating-autophagic-flux-during-spermiogenesis-in-mice
#5
Chao Liu, Zhenhua Song, Lina Wang, Haiyan Yu, Weixiao Liu, Yongliang Shang, Zhiliang Xu, Haichao Zhao, Fengyi Gao, Jiamin Wen, Linan Zhao, Yaoting Gui, Jianwei Jiao, Fei Gao, Wei Li
Sirt1 is a member of the sirtuin family of proteins and has important roles in numerous biological processes. Sirt1(-/-) mice display an increased frequency of abnormal spermatozoa, but the mechanism of Sirt1 in spermiogenesis remains largely unknown. Here, we report that Sirt1 might be directly involved in spermiogenesis in germ cells but not in steroidogenic cells. Germ cell-specific Sirt1 knockout mice were almost completely infertile; the early mitotic and meiotic progression of germ cells in spermatogenesis were not obviously affected after Sirt1 depletion, but subsequent spermiogenesis was disrupted due to a defect in acrosome biogenesis, which resulted in a phenotype similar to that observed in human globozoospermia...
December 21, 2016: Development
https://www.readbyqxmd.com/read/27965440/cfap157-is-a-murine-downstream-effector-of-foxj1-that-is-specifically-required-for-flagellum-morphogenesis-and-sperm-motility
#6
Marina Weidemann, Karin Schuster-Gossler, Michael Stauber, Christoph Wrede, Jan Hegermann, Tim Ott, Karsten Boldt, Tina Beyer, Katrin Serth, Elisabeth Kremmer, Martin Blum, Marius Ueffing, Achim Gossler
Motile cilia move extracellular fluids or mediate cellular motility. Their function is essential for embryonic development, adult tissue homeostasis and reproduction throughout vertebrates. FOXJ1 is a key transcription factor for the formation of motile cilia but its downstream genetic programme is only partially understood. Here, we characterise a novel FOXJ1 target, Cfap157, that is specifically expressed in motile ciliated tissues in mouse and Xenopus in a FOXJ1-dependent manner. CFAP157 protein localises to basal bodies and interacts with tubulin and the centrosomal protein CEP350...
December 15, 2016: Development
https://www.readbyqxmd.com/read/27939748/the-length-of-a-short-sperm-elongation-and-shortening-during-spermiogenesis-in-cotesia-congregata-hymenoptera-braconidae
#7
Rustem Uzbekov, Julien Burlaud-Gaillard, Anastasiia S Garanina, Christophe Bressac
The spermatozoon of the parasitoid wasp Cotesia congregata is an extremely short gamete measuring less than 7 μm; it is as yet the shortest flagellated sperm to be identified. The mature sperm consists of an acrosome, surrounded by an extra cellular coat, a condensed nucleus, two uncoiled mitochondrial derivatives and a short axoneme. Testes of young adults contain a continuum of differentiation stages. Initially, the flagellum is approximately 5 μm long. It conserves its length in round, elongated and mature spermatids, but is reduced to less than 3 μm in mature spermatozoa...
January 4, 2017: Arthropod Structure & Development
https://www.readbyqxmd.com/read/27920259/hormone-sensitive-lipase-deficiency-alters-gene-expression-and-cholesterol-content-of-mouse-testis
#8
Feng Wang, Zheng Chen, Xiaofang Ren, Ye Tian, Fucheng Wang, Chao Liu, Pengcheng Jin, Zongyue Li, Feixiong Zhang, Baochang Zhu
Hormone-sensitive lipase-knockout (HSL-/-) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes...
February 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27905325/the-impact-of-autophagy-in-spermiogenesis
#9
Nihan Ozturk, Klaus Steger, Undraga Schagdarsurengin
No abstract text is available yet for this article.
November 29, 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27896558/h3k9ac-involved-in-the-decondensation-of-spermatozoal-nuclei-during-spermatogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#10
Genliang Li, Xianjiang Kang, Shumei Mu, Mingshen Guo, Shiwen Huang, Qinna Chen, Song Nong, Xiaomin Huang, Hongliu Hu, Ke Sun
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27862212/comparative-morphology-of-the-reproductive-system-of-seven-species-of-ostariophysan-fishes-from-the-upper-das-velhas-river-brazil
#11
Rafael Magno Costa Melo, Débora Diniz Gomes, Davidson Peruci Moreira, Maysa Regina Gomes, Nilo Bazzoli, Elizete Rizzo
The success of fishes in different environments is related with the variation of reproductive strategies developed by the systematic group, which is reflected in the morphology of the reproductive system and can have ecological and evolutionary implications. This study comparatively analyzed the morphological characteristics of the male and female reproductive systems of ostariophysan fish species from the upper Das Velhas River in the São Francisco River basin, Brazil. In order to accomplish this, 393 specimens belonging to seven fish species were sampled between April 2010 and June 2015 for histological, ultrastructural, histochemical, and morphometric analyses...
November 8, 2016: Journal of Morphology
https://www.readbyqxmd.com/read/27854341/sept12-ndc1-complexes-are-required-for-mammalian-spermiogenesis
#12
Tsung-Hsuan Lai, Ying-Yu Wu, Ya-Yun Wang, Mei-Feng Chen, Pei Wang, Tsung-Ming Chen, Yi-No Wu, Han-Sun Chiang, Pao-Lin Kuo, Ying-Hung Lin
Male factor infertility accounts for approximately 50 percent of infertile couples. The male factor-related causes of intracytoplasmic sperm injection failure include the absence of sperm, immotile sperm, immature sperm, abnormally structured sperm, and sperm with nuclear damage. Our knockout and knock-in mice models demonstrated that SEPTIN12 (SEPT12) is vital for the formation of sperm morphological characteristics during spermiogenesis. In the clinical aspect, mutated SEPT12 in men results in oligozoospermia or teratozoospermia or both...
November 16, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27835637/transcriptomic-variation-during-spermiogenesis-in-mouse-germ-cells
#13
Haiyang Zuo, Junfang Zhang, Liuguang Zhang, Xiaoxia Ren, Xiaoli Chen, Haisheng Hao, Xueming Zhao, Dong Wang
To explore variations in the transcription activity during spermiogenesis, round and elongated spermatids were collected from ICR/CD1 model mice using laser capture microdissection (LCM) and cauda epididymal sperm samples. The transcripts were sequenced using RNA-seq, and the reads were mapped to mm9. The majority of the reads (70%) in the round and elongated spermatids were mappable to known and predicted exons, but that in sperm was only 9%. The results of the distribution of reads suggested that alternative splicing was more complicated in sperm than in round and elongated spermatids...
2016: PloS One
https://www.readbyqxmd.com/read/27831554/the-control-of-male-fertility-by-spermatid-specific-factors-searching-for-contraceptive-targets-from-spermatozoon-s-head-to-tail
#14
REVIEW
Su-Ren Chen, Aalia Batool, Yu-Qian Wang, Xiao-Xia Hao, Chawn-Shang Chang, C Yan Cheng, Yi-Xun Liu
Male infertility due to abnormal spermatozoa has been reported in both animals and humans, but its pathogenic causes, including genetic abnormalities, remain largely unknown. On the other hand, contraceptive options for men are limited, and a specific, reversible and safe method of male contraception has been a long-standing quest in medicine. Some progress has recently been made in exploring the effects of spermatid-specifical genetic factors in controlling male fertility. A comprehensive search of PubMed for articles and reviews published in English before July 2016 was carried out using the search terms 'spermiogenesis failure', 'globozoospermia', 'spermatid-specific', 'acrosome', 'infertile', 'manchette', 'sperm connecting piece', 'sperm annulus', 'sperm ADAMs', 'flagellar abnormalities', 'sperm motility loss', 'sperm ion exchanger' and 'contraceptive targets'...
November 10, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27810970/the-drosophila-chromosomal-protein-mst77f-is-processed-to-generate-an-essential-component-of-mature-sperm-chromatin
#15
Shuhei Kimura, Benjamin Loppin
In most animals, the bulk of sperm DNA is packaged with sperm nuclear basic proteins (SNBPs), a diverse group of highly basic chromosomal proteins notably comprising mammalian protamines. The replacement of histones with SNBPs during spermiogenesis allows sperm DNA to reach an extreme level of compaction, but little is known about how SNBPs actually function in vivo Mst77F is a Drosophila SNBP with unique DNA condensation properties in vitro, but its role during spermiogenesis remains unclear. Here, we show that Mst77F is required for the compaction of sperm DNA and the production of mature sperm, through its cooperation with protamine-like proteins Mst35Ba/b...
November 2016: Open Biology
https://www.readbyqxmd.com/read/27803675/subcellular-evidence-for-biogenesis-of-autophagosomal-membrane-during-spermiogenesis-in-vivo
#16
Yufei Huang, Ping Yang, Tengfei Liu, Hong Chen, Xiaoya Chu, Nisar Ahmad, Qian Zhang, Quanfu Li, Lisi Hu, Yi Liu, Qiusheng Chen
Although autophagosome formation has attracted substantial attention, the origin and the source of the autophagosomal membrane remains unresolved. The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27742209/knockdown-of-dynamitin-in-testes-significantly-decreased-male-fertility-in-drosophila-melanogaster
#17
Chun-Hong Wu, Qiong Zong, An-Li Du, Wei Zhang, Han-Chao Yao, Xiao-Qiang Yu, Yu-Feng Wang
Dynamitin (Dmn) is a major component of dynactin, a multiprotein complex playing important roles in a variety of intracellular motile events. We previously found that Wolbachia bacterial infection resulted in a reduction of Dmn protein. As Wolbachia may modify sperm in male hosts, we speculate that Dmn may have a function in male fertility. Here we used nosGal4 to drive Dmn knock down in testes of Drosophila melanogaster to investigate the functions of Dmn in spermatogenesis. We found that knockdown of Dmn in testes dramatically decreased male fertility, overexpression of Dmn in Wolbachia-infected males significantly rescued male fertility, indicating an important role of Dmn in inducing male fertility defects following Wolbachia infection...
December 1, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27717557/expression-of-katanin-p80-in-human-spermatogenesis
#18
Christiane Pleuger, Daniela Fietz, Katja Hartmann, Wolfgang Weidner, Sabine Kliesch, Moira K O'Bryan, Adriaan Dorresteijn, Martin Bergmann
OBJECTIVE: To define the stage-by-stage expression of KATNB1 during human spermatogenesis. DESIGN: Gene expression analysis, histologic and immunohistochemical evaluation. SETTING: University research laboratories and andrological clinic. PATIENT(S): Eighty human testicular biopsy samples: 43 showing normal spermatogenesis, 9 with maturation arrest at level of spermatocytes, 8 with maturation arrest at level of spermatogonia, and 20 with a Sertoli cell only syndrome...
December 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27698683/dna-methylation-in-spermatogenesis-and-male-infertility
#19
Xiangrong Cui, Xuan Jing, Xueqing Wu, Meiqin Yan, Qiang Li, Yan Shen, Zhenqiang Wang
Infertility is a significant problem for human reproduction, with males and females equally affected. However, the molecular mechanisms underlying male infertility remain unclear. Spermatogenesis is a highly complex process involving mitotic cell division, meiosis cell division and spermiogenesis; during this period, unique and extensive chromatin and epigenetic modifications occur to bring about specific epigenetic profiles in spermatozoa. It has recently been suggested that the dysregulation of epigenetic modifications, in particular the methylation of sperm genomic DNA, may serve an important role in the development of numerous diseases...
October 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27682589/intraflagellar-transport-protein-ift20-is-essential-for-male-fertility-and-spermiogenesis-in-mice
#20
Zhengang Zhang, Wei Li, Yong Zhang, Ling Zhang, Maria E Teves, Hong Liu, Jerome F Strauss, Gregory J Pazour, James A Foster, Rex A Hess, Zhibing Zhang
Intraflagellar transport (IFT) is a conserved mechanism thought to be essential for the assembly and maintenance of cilia and flagella. However, little is known about its role in mammalian sperm flagella formation. To fill this gap, we disrupted the Ift20 gene in male germ cells. Homozygous mutant mice were infertile with significantly reduced sperm counts and motility. In addition, abnormally shaped elongating spermatid heads and bulbous round spermatids were found in the lumen of the seminiferous tubules...
September 28, 2016: Molecular Biology of the Cell
keyword
keyword
40927
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"