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https://www.readbyqxmd.com/read/28336210/burkholderia-pseudomallei-and-burkholderia-mallei-vaccines-are-we-close-to-clinical-trials
#1
REVIEW
Richard W Titball, Mary N Burtnick, Gregory J Bancroft, Paul Brett
B. pseudomallei is the cause of melioidosis, a serious an often fatal disease of humans and animals. The closely related bacterium B. mallei, which cases glanders, is considered to be a clonal derivative of B. pseudomallei. Both B. pseudomallei and B. mallei were evaluated by the United States and the former USSR as potential bioweapons. Much of the effort to devise biodefence vaccines in the past decade has been directed towards the identification and formulation of sub-unit vaccines which could protect against both melioidosis and glanders...
March 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28330585/molecular-detection-of-leptospirosis-and-melioidosis-co-infection-a-case-report
#2
Mohammad Ridhuan Mohd Ali, Amira Wahida Mohamad Safiee, Padmaloseni Thangarajah, Mohd Hashairi Fauzi, Alwi Muhd Besari, Nabilah Ismail, Chan Yean Yean
Leptospirosis and melioidosis are important tropical infections caused by Leptospira and Burkholdheria pseudomallei, respectively. As both infections share similar clinical manifestations yet require different managements, complementary laboratory tests are crucial for the diagnosis. We describe a case of Leptospira and B. pseudomallei co-infection in a diabetic 40-year-old woman with history of visit to a freshwater camping site in northern Malaysia. To our knowledge, this is the first case of such double-infection, simultaneously demonstrated by molecular approach...
March 15, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28323523/rapid-dna-vaccination-against-burkholderia-pseudomallei-flagellin-by-tattoo-or-intranasal-application
#3
Jacqueline M Lankelma, Alex Wagemakers, Emma Birnie, Bastiaan W Haak, Jos J A Trentelman, Tassili A F Weehuizen, Jasmin Ersöz, Joris J T H Roelofs, Joppe W Hovius, W Joost Wiersinga, Adriaan D Bins
Melioidosis is a severe infectious disease with a high mortality that is endemic in South-East Asia and Northern Australia. The causative pathogen, Burkholderia pseudomallei, is listed as potential bioterror weapon due to its high virulence and potential for easy dissemination. Currently, there is no licensed vaccine for prevention of melioidosis. Here, we explore the use of rapid plasmid DNA vaccination against B. pseudomallei flagellin for protection against respiratory challenge. We tested three flagellin DNA vaccines with different subcellular targeting designs...
March 21, 2017: Virulence
https://www.readbyqxmd.com/read/28296884/increased-von-willebrand-factor-decreased-adamts13-and-thrombocytopenia-in-melioidosis
#4
Emma Birnie, Gavin C K W Koh, Ester C Löwenberg, Joost C M Meijers, Rapeephan R Maude, Nicholas P J Day, Sharon J Peacock, Tom van der Poll, W Joost Wiersinga
BACKGROUND: Melioidosis, caused by bioterror treat agent Burkholderia pseudomallei, is an important cause of community-acquired Gram-negative sepsis in Southeast Asia and Northern Australia. New insights into the pathogenesis of melioidosis may help improve treatment and decrease mortality rates from this dreadful disease. We hypothesized that changes in Von Willebrand factor (VWF) function should occur in melioidosis, based on the presence of endothelial stimulation by endotoxin, pro-inflammatory cytokines and thrombin in melioidosis, and investigated whether this impacted on outcome...
March 15, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28296377/-melioidosis-report-of-the-first-case-in-a-northern-state-of-m%C3%A3-xico
#5
Carlos Boroel-Cervantes, Mónica Ibarra-Valdez, Sandra Miranda-Pacheco, Elías Sánchez-Camarena, Tamara Wolburgth-Franco, Andrés Ortìz-González, José Luis Domínguez-Ríos
The melioidosis, is an infection caused by Burkhordelia pseudomallei that comprises heterogeneous clinical syndromes with acute or chronic evolution. The objective of this article is to report a case with unusual presentation and outside the known epidemiological context. We present the case of a 48-year-old man who came to our hospital with fever and a history of an abscess in the right subscapular region, physical examination showed hepatomegaly, splenomegaly, fever, without pulmonary symptoms. Within study it reveal multiple abscesses in liver and spleen, requiring surgical exploration and antibiotics (meropenem/vancomycin)...
March 2017: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/28283532/molecular-architecture-of-the-n-type-atpase-rotor-ring-from-burkholderia-pseudomallei
#6
Sarah Schulz, Martin Wilkes, Deryck J Mills, Werner Kühlbrandt, Thomas Meier
The genome of the highly infectious bacterium Burkholderia pseudomallei harbors an atp operon that encodes an N-type rotary ATPase, in addition to an operon for a regular F-type rotary ATPase. The molecular architecture of N-type ATPases is unknown and their biochemical properties and cellular functions are largely unexplored. We studied the B. pseudomallei N1No-type ATPase and investigated the structure and ion specificity of its membrane-embedded c-ring rotor by single-particle electron cryo-microscopy. Of several amphiphilic compounds tested for solubilizing the complex, the choice of the low-density, low-CMC detergent LDAO was optimal in terms of map quality and resolution...
March 10, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28272358/comparison-of-the-sulfonamide-inhibition-profiles-of-the-%C3%AE-and-%C3%AE-carbonic-anhydrases-from-the-pathogenic-bacterium-burkholderia-pseudomallei
#7
Daniela Vullo, Sonia Del Prete, Pietro Di Fonzo, Vincenzo Carginale, W Alexander Donald, Claudiu T Supuran, Clemente Capasso
We have cloned, purified, and characterized a β-carbonic anhydrase (CA, EC 4.2.1.1), BpsCAβ, from the pathogenic bacterium Burkholderia pseudomallei, responsible for the tropical disease melioidosis. The enzyme showed high catalytic activity for the physiologic CO₂ hydration reaction to bicarbonate and protons, with the following kinetic parameters: kcat of 1.6 × 10⁵ s(-1) and kcat/KM of 3.4 × 10⁷ M(-1) s(-1). An inhibition study with a panel of 38 sulfonamides and one sulfamate-including 15 compounds that are used clinically-revealed an interesting structure-activity relationship for the interaction of this enzyme with these inhibitors...
March 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28264029/the-epidemiology-and-clinical-features-of-melioidosis-in-far-north-queensland-implications-for-patient-management
#8
James D Stewart, Simon Smith, Enzo Binotto, William J McBride, Bart J Currie, Josh Hanson
BACKGROUND: The epidemiology, clinical presentation and management of melioidosis vary around the world. It is essential to define the disease's local features to optimise its management. PRINCIPAL FINDINGS: Between 1998 and 2016 there were 197 cases of culture confirmed melioidosis in Far North Queensland; 154 (78%) presented in the December-April wet season. 145 (74%) patients were bacteraemic, 58 (29%) were admitted to the Intensive Care Unit and 27 (14%) died; nine (33%) of these deaths occurred within 48 hours of presentation...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28250624/septicemic-melioidosis-with-ruptured-splenic-abscess-in-a-patient-with-thalassemia-intermedia
#9
Angel T Miraclin, Selvin Sundar Raj Mani, Sandhya Suresh, Ramya Iyyadurai
No abstract text is available yet for this article.
January 2017: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/28238511/comparison-of-the-anion-inhibition-profiles-of-the-%C3%AE-and-%C3%AE-carbonic-anhydrases-from-the-pathogenic-bacterium-burkholderia-pseudomallei
#10
Sonia Del Prete, Daniela Vullo, Pietro di Fonzo, Vincenzo Carginale, Claudiu T Supuran, Clemente Capasso
We report the cloning, purification and characterization of BpsβCA, a β-class carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei, the etiological agent of melioidosis, and compare its activity and inhibition with those of the γ-CA from the same organism, BpsγCA, recently investigated by our groups. BpsβCA showed a significant catalytic activity for the physiologic, CO2 hydration reaction, with the following kinetic parameters, kcat of 1.6×10(5)s(-1) and kcat/Km of 3...
February 16, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28235984/-chronic-skin-ulcer-in-a%C3%A2-24-year-old-man-after-a%C3%A2-journey-to-southeast-asia
#11
A Rueda Guzmán, G Slesak, R Fleck, R Ignatius, R Oehme, J Schäfer
Our report concerns a 24-year-old man with a chronic exsudative skin lesion after a journey to Southeast Asia. The diagnosis of melioidosis was made by the identification of Burkholderia pseudomallei from the ichor. The diagnosis was confirmed by polymerase change reaction. The patient was treated with meropenem i. v. for about 10 days and with trimethoprim/sulfamethoxazole for the following 12 weeks. Melioidosis is an endemic disease in Southeast Asia and North Australia which in some cases can run a severe course and can have a high fatality rate...
February 24, 2017: Der Internist
https://www.readbyqxmd.com/read/28216665/interleukin-10-inhibits-pro-inflammatory-cytokine-responses-and-killing-of-burkholderia-pseudomallei
#12
Bianca Kessler, Darawan Rinchai, Chidchamai Kewcharoenwong, Arnone Nithichanon, Rachael Biggart, Catherine M Hawrylowicz, Gregory J Bancroft, Ganjana Lertmemongkolchai
Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28188208/quantitative-detection-of-burkholderia-pseudomallei-in-soil-samples-using-multiple-gene-targets-in-real-time-pcr
#13
A Göhler, T T Trung, V Hopf, C Kohler, J Hartleib, V Wuthiekanun, S J Peacock, D Limmathurotsakul, A Tuanyok, I Steinmetz
Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment is a prerequisite to assess the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a qPCR targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from comparative genomic analysis...
February 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28187198/two-stable-variants-of-burkholderia-pseudomallei-strain-mshr5848-express-broadly-divergent-in-vitro-phenotypes-associated-with-their-virulence-differences
#14
A A Shea, R C Bernhards, C K Cote, C J Chase, J W Koehler, C P Klimko, J T Ladner, D A Rozak, M J Wolcott, D P Fetterer, S J Kern, G I Koroleva, S P Lovett, G F Palacios, R G Toothman, J A Bozue, P L Worsham, S L Welkos
Burkholderia pseudomallei (Bp), the agent of melioidosis, causes disease ranging from acute and rapidly fatal to protracted and chronic. Bp is highly infectious by aerosol, can cause severe disease with nonspecific symptoms, and is naturally resistant to multiple antibiotics. However, no vaccine exists. Unlike many Bp strains, which exhibit random variability in traits such as colony morphology, Bp strain MSHR5848 exhibited two distinct and relatively stable colony morphologies on sheep blood agar plates: a smooth, glossy, pale yellow colony and a flat, rough, white colony...
2017: PloS One
https://www.readbyqxmd.com/read/28179407/calprotectin-as-a-biomarker-for-melioidosis-disease-progression-and-management
#15
Mohan Natesan, Enoka Corea, Shivankari Krishnananthasivam, Harindra Darshana Sathkumara, Jennifer L Dankmeyer, Beverly K Dyas, Kei Amemiya, Aruna Dharshan De Silva, Robert G Ulrich
Melioidosis is a neglected tropical disease that is caused by the bacterium Burkholderia pseudomallei, and is underreported in many endemic countries. A long and costly administration of antibiotics is required to clear infections, and there is an unmet need for biomarkers to guide antibiotic treatment and increase the number of patients that complete therapy. We identified calprotectin as a lead biomarker of Burkholderia pseudomallei infections, and examined correlations between this serum protein and antibiotic treatment outcomes of melioidosis patients...
February 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28177319/expression-and-crystallographic-studies-of-d-glycero-%C3%AE-d-manno-heptose-1-phosphate-adenylyltransferase-from-burkholderia-pseudomallei
#16
Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Dong Hae Shin
The Gram-negative bacterium Burkholderia pseudomallei is the causative agent of melioidosis. D-glycero-β-D-manno-Heptose-1-phosphate adenylyltransferase (HldC) is the fourth enzyme of the ADP-L-glycero-β-D-manno-heptose biosynthesis pathway, which produces an essential carbohydrate comprising the inner core of lipopolysaccharide. Therefore, HldC is a potential target of antibiotics against melioidosis. In this study, HldC from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were also collected to 2...
February 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28166079/osteomyelitis-of-humerus-and-intramuscular-abscess-due-to-melioidosis
#17
G S Vijaykumar, P Thilakavathy, S S Jeremiah, G Vithiya
Melioidosis is a clinically diverse disease caused by gram negative bacterium Burkholderia pseudomallei. It is a potential bioterrorism agent. The high risk group includes the agricultural and construction workers whose contact with contaminated soil and water may expose them to bacteria. The clinical manifestations varies from asymptomatic infection to overwhelming sepsis. To diagnose melioidosis a high index of suspicion along with isolation and identification of the organism from the clinical samples is needed...
April 2016: Kathmandu University Medical Journal (KUMJ)
https://www.readbyqxmd.com/read/28141587/osteoarticular-and-soft-tissue-melioidosis-in-malaysia-clinical-characteristics-and-molecular-typing-of-the-causative-agent
#18
AbdelRahman Mohammad Zueter, Mahmoud Abumarzouq, Mohd Imran Yusof, Wan Faisham Wan Ismail, Azian Harun
INTRODUCTION: Melioidosis involving bone, joints, and soft tissue is rare and reported usually following dissemination of disease from infection elsewhere in the body; to a lesser degree, it can also be reported as the primary manifestation of melioidosis. METHODOLOGY: The orthopedic registry at Hospital University Sains Malaysia from 2008 until 2014 was retrospectively reviewed and was followed by molecular typing of Burkholderia pseudomallei. RESULTS: Out of 20 cases identified, 19 patients were confirmed to have osteoarticular and/or soft-tissue melioidosis...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28134016/multiple-visceral-abscesses-in-a-patient-with-diabetes-mellitus-a-rare-yet-corrigible-infection-melioidosis
#19
Anil Satayraddi, Kripa Elizabeth Cherian, Nitin Kapoor, Priscilla Rupali, Thomas Vizhalil Paul
No abstract text is available yet for this article.
January 1, 2017: Tropical Doctor
https://www.readbyqxmd.com/read/28115683/validation-of-a-monoclonal-antibody-based-immunofluorescent-assay-to-detect-burkholderia-pseudomallei-in-blood-cultures
#20
Adul Dulsuk, Suporn Paksanont, Adisak Sangchankoom, Peeraya Ekchariyawat, Rungnapa Phunpang, Yaowaruk Jutrakul, Narisara Chantratita, T Eoin West
BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99...
January 22, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
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