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https://www.readbyqxmd.com/read/28216665/interleukin-10-inhibits-pro-inflammatory-cytokine-responses-and-killing-of-burkholderia-pseudomallei
#1
Bianca Kessler, Darawan Rinchai, Chidchamai Kewcharoenwong, Arnone Nithichanon, Rachael Biggart, Catherine M Hawrylowicz, Gregory J Bancroft, Ganjana Lertmemongkolchai
Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28188208/quantitative-detection-of-burkholderia-pseudomallei-in-soil-samples-using-multiple-gene-targets-in-real-time-pcr
#2
A Göhler, T T Trung, V Hopf, C Kohler, J Hartleib, V Wuthiekanun, S J Peacock, D Limmathurotsakul, A Tuanyok, I Steinmetz
Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment is a prerequisite to assess the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a qPCR targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from comparative genomic analysis...
February 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28187198/two-stable-variants-of-burkholderia-pseudomallei-strain-mshr5848-express-broadly-divergent-in-vitro-phenotypes-associated-with-their-virulence-differences
#3
A A Shea, R C Bernhards, C K Cote, C J Chase, J W Koehler, C P Klimko, J T Ladner, D A Rozak, M J Wolcott, D P Fetterer, S J Kern, G I Koroleva, S P Lovett, G F Palacios, R G Toothman, J A Bozue, P L Worsham, S L Welkos
Burkholderia pseudomallei (Bp), the agent of melioidosis, causes disease ranging from acute and rapidly fatal to protracted and chronic. Bp is highly infectious by aerosol, can cause severe disease with nonspecific symptoms, and is naturally resistant to multiple antibiotics. However, no vaccine exists. Unlike many Bp strains, which exhibit random variability in traits such as colony morphology, Bp strain MSHR5848 exhibited two distinct and relatively stable colony morphologies on sheep blood agar plates: a smooth, glossy, pale yellow colony and a flat, rough, white colony...
2017: PloS One
https://www.readbyqxmd.com/read/28179407/calprotectin-as-a-biomarker-for-melioidosis-disease-progression-and-management
#4
Mohan Natesan, Enoka Corea, Shivankari Krishnananthasivam, Harindra Darshana Sathkumara, Jennifer L Dankmeyer, Beverly K Dyas, Kei Amemiya, Aruna Dharshan De Silva, Robert G Ulrich
Melioidosis is a neglected tropical disease that is caused by the bacterium Burkholderia pseudomallei, and is underreported in many endemic countries. A long and costly administration of antibiotics is required to clear infections, and there is an unmet need for biomarkers to guide antibiotic treatment and increase the number of patients that complete therapy. We identified calprotectin as a lead biomarker of Burkholderia pseudomallei infections, and examined correlations between this serum protein and antibiotic treatment outcomes of melioidosis patients...
February 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28177319/expression-and-crystallographic-studies-of-d-glycero-%C3%AE-d-manno-heptose-1-phosphate-adenylyltransferase-from-burkholderia-pseudomallei
#5
Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Dong Hae Shin
The Gram-negative bacterium Burkholderia pseudomallei is the causative agent of melioidosis. D-glycero-β-D-manno-Heptose-1-phosphate adenylyltransferase (HldC) is the fourth enzyme of the ADP-L-glycero-β-D-manno-heptose biosynthesis pathway, which produces an essential carbohydrate comprising the inner core of lipopolysaccharide. Therefore, HldC is a potential target of antibiotics against melioidosis. In this study, HldC from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were also collected to 2...
February 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28166079/osteomyelitis-of-humerus-and-intramuscular-abscess-due-to-melioidosis
#6
G S Vijaykumar, P Thilakavathy, S S Jeremiah, G Vithiya
Melioidosis is a clinically diverse disease caused by gram negative bacterium Burkholderia pseudomallei. It is a potential bioterrorism agent. The high risk group includes the agricultural and construction workers whose contact with contaminated soil and water may expose them to bacteria. The clinical manifestations varies from asymptomatic infection to overwhelming sepsis. To diagnose melioidosis a high index of suspicion along with isolation and identification of the organism from the clinical samples is needed...
April 2016: Kathmandu University Medical Journal (KUMJ)
https://www.readbyqxmd.com/read/28141587/osteoarticular-and-soft-tissue-melioidosis-in-malaysia-clinical-characteristics-and-molecular-typing-of-the-causative-agent
#7
AbdelRahman Mohammad Zueter, Mahmoud Abumarzouq, Mohd Imran Yusof, Wan Faisham Wan Ismail, Azian Harun
INTRODUCTION: Melioidosis involving bone, joints, and soft tissue is rare and reported usually following dissemination of disease from infection elsewhere in the body; to a lesser degree, it can also be reported as the primary manifestation of melioidosis. METHODOLOGY: The orthopedic registry at Hospital University Sains Malaysia from 2008 until 2014 was retrospectively reviewed and was followed by molecular typing of Burkholderia pseudomallei. RESULTS: Out of 20 cases identified, 19 patients were confirmed to have osteoarticular and/or soft-tissue melioidosis...
January 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28134016/multiple-visceral-abscesses-in-a-patient-with-diabetes-mellitus-a-rare-yet-corrigible-infection-melioidosis
#8
Anil Satayraddi, Kripa Elizabeth Cherian, Nitin Kapoor, Priscilla Rupali, Thomas Vizhalil Paul
No abstract text is available yet for this article.
January 1, 2017: Tropical Doctor
https://www.readbyqxmd.com/read/28115683/validation-of-a-monoclonal-antibody-based-immunofluorescent-assay-to-detect-burkholderia-pseudomallei-in-blood-cultures
#9
Adul Dulsuk, Suporn Paksanont, Adisak Sangchankoom, Peeraya Ekchariyawat, Rungnapa Phunpang, Yaowaruk Jutrakul, Narisara Chantratita, T Eoin West
BACKGROUND: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. METHODS: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. RESULTS: Sensitivity of the IFA was 100% and specificity was 99...
January 22, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28112723/global-and-regional-dissemination-and-evolution-of-burkholderia-pseudomallei
#10
Claire Chewapreecha, Matthew T G Holden, Minna Vehkala, Niko Välimäki, Zhirong Yang, Simon R Harris, Alison E Mather, Apichai Tuanyok, Birgit De Smet, Simon Le Hello, Chantal Bizet, Mark Mayo, Vanaporn Wuthiekanun, Direk Limmathurotsakul, Rattanaphone Phetsouvanh, Brian G Spratt, Jukka Corander, Paul Keim, Gordon Dougan, David A B Dance, Bart J Currie, Julian Parkhill, Sharon J Peacock
The environmental bacterium Burkholderia pseudomallei causes an estimated 165,000 cases of human melioidosis per year worldwide and is also classified as a biothreat agent. We used whole genome sequences of 469 B. pseudomallei isolates from 30 countries collected over 79 years to explore its geographic transmission. Our data point to Australia as an early reservoir, with transmission to Southeast Asia followed by onward transmission to South Asia and East Asia. Repeated reintroductions were observed within the Malay Peninsula and between countries bordered by the Mekong River...
January 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28105344/molecular-tracking-investigation-of-melioidosis-cases-reveals-regional-endemicity-in-hainan-china
#11
Yingzi Lin, Qiang Wu, Xiang Liu, Sufang Dong, Lixian Wu, Hua Pei, Ke Xu, Qianfeng Xia
Sporadic cases of melioidosis have been reported in Hainan, China for decades; however, to the best of our knowledge, there are no accurate source-identification investigations confirming that melioidosis is endemic. Four indigenous melioidosis cases were identified, which prompted the performance of contact microbiologic and molecular techniques to evaluate endemicity. Environmental samples were collected from various locations surrounding each patient's residence. The samples were screened for Burkholderia pseudomallei (B...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28096229/disseminated-melioidosis-in-the-head-and-neck
#12
Tze Ling Loh, Sergios Latis, Graeme Crossland, Hemi Patel
A 35-year-old man was admitted to an intensive care unit with unilateral facial swelling and septic shock after multiple presentations to the emergency department with non-specific unilateral pain over the parotid area. A CT scan of his neck showed diffuse right-sided facial soft tissue infection, mastoid effusion and temporal lobe cerebritis. The upper lobes of his lungs had cannonball lesions that were suggestive of septic lung metastases. Blood cultures and ear canal swabs were positive for Burkholderia pseudomallei The temporal lobe cerebritis eventually developed into an abscess, necessitating a cortical mastoidectomy, craniectomy and temporal lobectomy...
January 17, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28078837/endocarditis-in-melioidosis
#13
L B Piyasiri, S A Wickramasinghe, V C Lekamvasam, E M Corea, R Gunarathne, U Priyadarshana
No abstract text is available yet for this article.
30, 2016: Ceylon Medical Journal
https://www.readbyqxmd.com/read/28066900/boosting-of-post-exposure-human-t-cell-and-b-cell-recall-responses-in-vivo-by-burkholderia-pseudomallei-related-proteins
#14
Arnone Nithichanon, Louise J Gourlay, Gregory J Bancroft, Manabu Ato, Yoshimasa Takahashi, Ganjana Lertmemongkolchai
Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease with high incidence and mortality in South East Asia and northern Australia. To date there is no protective vaccine and antibiotic treatment is prolonged and not always effective. Most people living in endemic areas have been exposed to the bacteria and have developed some immunity, which may have helped to prevent disease. Here, we used a humanized mouse model (hu-PBL-SCID), reconstituted with human peripheral blood mononuclear cells from seropositive donors, to illustrate the potential of three known antigens (FliC, OmpA and N-PilO2) for boosting both T-cell and B-cell immune responses...
January 9, 2017: Immunology
https://www.readbyqxmd.com/read/28045926/survival-and-intra-nuclear-trafficking-of-burkholderia-pseudomallei-strategies-of-evasion-from-immune-surveillance
#15
Jamuna Vadivelu, Kumutha Malar Vellasamy, Jaikumar Thimma, Vanitha Mariappan, Wen-Tyng Kang, Leang-Chung Choh, Esaki M Shankar, Kum Thong Wong
BACKGROUND: During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei) reside/hide to escape from host immune sensors and antimicrobial pressure. METHODS: We used transmission electron microscopy (TEM) to investigate post-mortem tissue sections of patients with clinical melioidosis to identify the localisation of a recently identified gut microbiome, B...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28042405/imported-case-of-melioidosis-in-oman-case-report
#16
Nada Al Tamtami, Faryal Khamis, Amina Al-Jardani
Melioidosis is an infectious disease caused by the bacterium Burkholderia pseudomallei. It is most commonly described in Southeast Asia and Northern Australia and some imported cases in non-endemic areas. We describe the case of a 55-year-old Omani man with fulminant sepsis who worked in Laos, Cambodia. B. pseudomallei was isolated from the patient's blood and was identified by means of microbiological and biochemical tests. We highlight the importance of careful attention to non-fermentative gram-negative rods in a septic patients who have worked or travelled to Southeast Asia...
January 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/28028452/burkholderia-pseudomallei-type-iii-secreted-protein-bipc-role-in-actin-modulation-and-translocation-activities-required-for-the-bacterial-intracellular-lifecycle
#17
Wen Tyng Kang, Kumutha Malar Vellasamy, Lakshminarayanan Rajamani, Roger W Beuerman, Jamuna Vadivelu
Melioidosis, an infection caused by the facultative intracellular pathogen Burkholderia pseudomallei, has been classified as an emerging disease with the number of patients steadily increasing at an alarming rate. B. pseudomalleipossess various virulence determinants that allow them to invade the host and evade the host immune response, such as the type III secretion systems (TTSS). The products of this specialized secretion system are particularly important for the B. pseudomallei infection. Lacking in one or more components of the TTSS demonstrated different degrees of defects in the intracellular lifecycle of B...
2016: PeerJ
https://www.readbyqxmd.com/read/28025002/sulfonamide-inhibition-profile-of-the-%C3%AE-carbonic-anhydrase-identified-in-the-genome-of-the-pathogenic-bacterium-burkholderia-pseudomallei-the-etiological-agent-responsible-of-melioidosis
#18
Sonia Del Prete, Daniela Vullo, Pietro Di Fonzo, Sameh M Osman, Zeid AlOthman, William A Donald, Claudiu T Supuran, Clemente Capasso
A new γ-carbonic anhydrase (CA, EC 4.1.1.1) was cloned and characterized kinetically in the genome of the bacterial pathogen Burkholderia pseudomallei, the etiological agent of melioidosis, an endemic disease of tropical and sub-tropical regions of the world. The catalytic activity of this new enzyme, BpsCAγ, is significant with a kcat of 5.3×10(5)s(-1) and kcat/Km of 2.5×10(7)M(-1)×s(-1) for the physiologic CO2 hydration reaction. The inhibition constant value for this enzyme for 39 sulfonamide inhibitors was obtained...
December 12, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28005910/melioidosis-in-malaysia-a-review-of-case-reports
#19
Paul Vijay Kingsley, Mark Leader, Nandika Suranjith Nagodawithana, Meghan Tipre, Nalini Sathiakumar
BACKGROUND: Melioidosis is a tropical infectious disease associated with significant mortality due to early onset of sepsis. OBJECTIVE: We sought to review case reports of melioidosis from Malaysia. METHODS: We conducted a computerized search of literature resources including PubMed, OVID, Scopus, MEDLINE and the COCHRANE database to identify published case reports from 1975 to 2015. We abstracted information on clinical characteristics, exposure history, comorbid conditions, management and outcome...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28003338/a-predicted-cation-transporter-protein-bpss1228-is-involved-in-intracellular-behaviour-of-burkholderia-pseudomallei-in-a-human-lung-epithelial-cell-line-a549
#20
Teerasit Techawiwattanaboon, Sorujsiri Chareonsudjai
Burkholderia pseudomallei causes melioidosis, a potentially fatal infectious disease in tropical and subtropical countries worldwide. The intracellular behaviour of this pathogen in host cells has been reported to impact the severity of melioidosis, including the development of septicaemia, a consequence of pneumonia melioidosis. We previously identified a predicted cation transporter protein, BPSS1228, that participates in the transitional stage of this intracellular pathogen. For further analysis, in this study B...
December 2016: FEMS Microbiology Letters
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