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Paulo Almeida lipid

Francis D O Ablan, B Logan Spaller, Kaitlyn I Abdo, Paulo F Almeida
Hundreds of cationic antimicrobial and cell-penetrating peptides (CPPs) form amphipathic α-helices when bound to lipid membranes. Here, we test two hypotheses for the differences in the ability of these peptides to translocate across membranes. The first, which we now call the hydrophobicity hypothesis, is that peptide translocation is determined by the Gibbs energy of insertion into the bilayer from the membrane interface. The second, which we call the charge-distribution hypothesis, is that translocation is determined by whether the distribution of cationic residues in the peptide can transiently stabilize a high-energy inserted intermediate by forming salt bridges to the phosphates of lipid headgroups...
October 18, 2016: Biophysical Journal
Mariah J King, Ashley L Bennett, Paulo F Almeida, Hee-Seung Lee
δ-lysin, secreted by a Gram-positive bacterium Staphylococcus aureus, is a 26-residue membrane active peptide that shares many common features with antimicrobial peptides (AMPs). However, it possesses a few unique features that differentiate itself from typical AMPs. In particular, δ-lysin has zero net charge, even though it has many charged residues, and it preferentially lyses eukaryotic cells over bacterial cells. Here, we present the results of coarse-grained molecular dynamics simulations of δ-lysin interacting with a zwitterionic membrane over a wide range of peptide concentrations...
October 6, 2016: Biochimica et Biophysica Acta
Bianca de Almeida-Pititto, Fernando Flexa Ribeiro-Filho, Sandhi Barreto, Bruce B Duncan, Maria Inês Schmidt, Paulo A Lotufo, Isabela M Bensenor, Sandra R G Ferreira
Objective: Our aim was to describe the distribution of selected biomarkers according to age and sex, adjusted for HOMA-IR and adiposity, in a subset of middle-aged individuals of Brazilian Longitudinal Study of Adult Health-ELSA without diabetes mellitus or CVD. Subjects and methods: This cross-sectional study was conducted in 998 participants of the ELSA-Brasil without diabetes and/or cardiovascular disease. In addition to the traditional risk factors, several biomarkers concentrations were compared according to sex, age groups (35-44; 45-54 yrs) and HOMA-IR tertiles...
September 5, 2016: Archives of Endocrinology and Metabolism
Luciana Gavilan Dias Folchetti, Isis Tande da Silva, Bianca de Almeida-Pititto, Sandra Roberta G Ferreira
INTRODUCTION: Non-communicable chronic diseases (NCCDs) represent a burden for public health. Alongside the established cardiometabolic risk factors such as high blood pressure and disorders of glucose and lipid metabolism, living habits and nutritional status at different stages of life are seen as contributors to this scenario. Gut microbiota composition and subclinical inflammation have been pointed out as underlying mechanisms of NCCDs. Studies involving health professionals have brought relevant contributions to the knowledge about risk factors...
2016: BMJ Open
Bianca de Almeida-Pititto, Fernando Flexa Ribeiro-Filho, Itamar S Santos, Paulo A Lotufo, Isabela M Bensenor, Sandra Rg Ferreira
OBJECTIVE: The study assessed the association of adiponectin concentration with carotid intima-media thickness (CIMT) in middle-aged participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) without diabetes or cardiovascular disease. DESIGN: Cross-sectional analyses. METHODS: A sample of 687 individuals (35-54 years old) without diabetes or cardiovascular disease was stratified into two categories according to CIMT (< or ≥ 75th percentile)...
August 22, 2016: European Journal of Preventive Cardiology
Priscila Ortega, Hector A Vitorino, Renata G Moreira, Marcelo A A Pinheiro, Alaor A Almeida, Márcio R Custódio, Flávia P Zanotto
Crustaceans found in metal contaminated regions are able to survive and the authors decided to investigate the physiological mechanisms involved by comparing populations from contaminated and non-contaminated areas. The objective of the present study was to measure the cellular transport of a non-essential metal (Cd, cadmium), in gills (G) and hepatopancreas (H) of Ucides cordatus, together with cell membrane fluidity, metallothionein levels (MT) and lipid peroxidation (LPO). The two populations compared were from a polluted (P) and a non-polluted (NP) mangrove area of São Paulo State, Brazil...
June 22, 2016: Environmental Toxicology and Chemistry
Diana Couto, Marisa Freitas, Vera Marisa Costa, Renan Campos Chisté, Agostinho Almeida, M Arturo Lopez-Quintela, José Rivas, Paulo Freitas, Paula Silva, Félix Carvalho, Eduarda Fernandes
Iron oxide nanoparticles (IONs) have physical and chemical properties that render them useful for several new biomedical applications. Still, so far, in vivo safety studies of IONs with coatings of biomedical interest are still scarce. The aim of this study, therefore, was to clarify the acute biological effects of polyacrylic acid (PAA)-coated IONs, by determining their biodistribution and their potential proinflammatory and toxic effects in CD-1 mice. The biodistribution of PAA-coated IONs in several organs (liver, spleen, kidneys, brain, heart, testes and lungs), the plasma cytokines, chemokine and aminotransferases levels, white blood cell count, oxidative stress parameters, adenosine triphosphate and histologic features of liver, spleen and kidneys were evaluated 24 h after a single acute (8, 20 or 50 mg kg(-1) ) intravenous administration of PAA-coated IONs in magnetite form...
October 2016: Journal of Applied Toxicology: JAT
Miguel Henrique de Almeida Santana, Gerson Antônio Oliveira Junior, Aline Silva Mello Cesar, Mateus Castelani Freua, Rodrigo da Costa Gomes, Saulo da Luz E Silva, Paulo Roberto Leme, Heidge Fukumasu, Minos Esperândio Carvalho, Ricardo Vieira Ventura, Luiz Lehmann Coutinho, Haja N Kadarmideen, José Bento Sterman Ferraz
The use of genome-wide association results combined with other genomic approaches may uncover genes and metabolic pathways related to complex traits. In this study, the phenotypic and genotypic data of 1475 Nellore (Bos indicus) cattle and 941,033 single nucleotide polymorphisms (SNPs) were used for genome-wide association study (GWAS) and copy number variations (CNVs) analysis in order to identify candidate genes and putative pathways involved with the feed conversion ratio (FCR). The GWAS was based on the Bayes B approach analyzing genomic windows with multiple regression models to estimate the proportion of genetic variance explained by each window...
March 21, 2016: Journal of Applied Genetics
Leônia Maria Batista, Gedson Rodrigues De Morais Lima, Ana Beatriz Albino De Almeida, Luciana De Pietro Magri, Tamara Regina Calvo, Anderson Luiz Ferreira, Cláudia Helena Pellizzon, Clélia Akiko Hiruma-Lima, Wagner Vilegas, Paulo Takeo Sano, Alba Regina Monteiro Souza Brito
BACKGROUND: Syngonanthus arthrotrichus and Syngonanthus bisulcatus, currently known for Comanthera aciphylla (Bong.) L.R.Parra & Giul. and Comanthera bisulcata (Koern.) L.R. Parra & Giul, popularly known in Brazil as "sempre-vivas," are plants from the family Eriocaulaceae. They are found in the states of Minas Gerais and Bahia. The species are known to be rich in flavonoids to which their gastroprotective activity has been attributed. In this research, experimental protocols were performed to elucidate the associated mechanisms of action...
2015: BMC Complementary and Alternative Medicine
Patrícia Leal de Almeida, Silvério Nepomuceno de Lima, Luciene Lacerda Costa, Cintia Cristina de Oliveira, Karina Aparecida Damasceno, Bibiana Alves dos Santos, Paulo Cezar Bastianello Campagnol
This study investigated the lipid oxidation and the microbiological and sensory quality of Bologna-type sausages produced with the addition of jabuticaba peel extract (JPE). Instrumental parameters of color (L*, a* and b*), pH, thiobarbituric acid reactive substance (TBARS) values, microbiological profile, and sensory properties were determined during 35 days of storage. The addition of JPE had an effect on pH and protected the samples from color changes during storage. However, JPE had no positive effect on microbial stability during storage...
December 2015: Meat Science
Ana Rita O Rodrigues, I T Gomes, Bernardo G Almeida, J P Araújo, Elisabete M S Castanheira, Paulo J G Coutinho
Nickel ferrite nanoparticles with superparamagnetic behavior at room temperature were synthesized using a coprecipitation method. These magnetic nanoparticles were either covered with a lipid bilayer, forming dry magnetic liposomes (DMLs), or entrapped in liposomes, originating aqueous magnetoliposomes (AMLs). A new and promising method for the synthesis of DMLs is described. The presence of the lipid bilayer in DMLs was confirmed by FRET (Förster Resonance Energy Transfer) measurements between the fluorescent-labeled lipids NBD-C12-HPC (NBD acting as a donor) included in the second lipid layer and rhodamine B-DOPE (acceptor) in the first lipid layer...
July 21, 2015: Physical Chemistry Chemical Physics: PCCP
Mônica de Oliveira Belém, Carla Possani Cirilo, Ana Paula de Santi-Rampazzo, João Paulo Ferreira Schoffen, Jurandir Fernando Comar, Maria Raquel Marçal Natali, Eduardo José de Almeida Araújo
During the aging process, the body's systems change structurally and loss of function can occur. Ingesting a smaller amount of food has been considered a plausible proposal for increased longevity with the quality of life. However, the effects of dietary restriction (DR) during aging are still poorly understood, especially for organs of the digestive system. This study aimed to describe the body weight, oxidative status and possible morphological changes of the intestinal wall of rats submitted to DR during the aging process (7 to 18months old)...
September 2015: Experimental Gerontology
Luiz Henrique Soares de Andrade, Wilson Max Almeida Monteiro de Moraes, Eduardo Hiroshi Matsuo Junior, Elizabeth de Orleans Carvalho de Moura, Hanna Karen Moreira Antunes, Jairo Montemor, Ednei Luiz Antonio, Danilo Sales Bocalini, Andrey Jorge Serra, Paulo José Ferreira Tucci, Patricia Chakur Brum, Alessandra Medeiros
The activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. The aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wistar and SHR rats were randomly divided into sedentary and trained groups...
April 2015: Molecular and Cellular Biochemistry
Marcela Abbott Galvão Ururahy, Karla Simone Costa de Souza, Yonara Monique da Costa Oliveira, Melina Bezerra Loureiro, Heglayne Pereira Vital da Silva, Francisco Paulo Freire-Neto, João Felipe Bezerra, André Ducati Luchessi, Sonia Quateli Doi, Rosario Dominguez Crespo Hirata, Maria das Graças Almeida, Ricardo Fernando Arrais, Mario Hiroyuki Hirata, Adriana Augusto de Rezende
BACKGROUND: Pro-inflammatory cytokines, such as interleukin-6 (IL-6), have been considered as key factors in type 1 diabetes mellitus (T1DM) and diabetic nephropathy, thus, our aim was to investigate the association of IL6-174G>C (rs1800795) and -634C>G (rs1800796) polymorphisms with T1DM susceptibility and diabetic nephropathy. METHODS: These polymorphisms were analyzed in 144 children and adolescents with T1DM and 173 normoglycemic control subjects. Glycemic control, laboratory parameters of kidney function and serum lipids were evaluated...
July 2015: Diabetes/metabolism Research and Reviews
Hugo Almeida, Maria Helena Amaral, Paulo Lobão, Ana C Silva, José Manuel Sousa Loboa
The unique properties and characteristics of ocular tissues and the whole set of defence mechanisms of the ocular globe make the instillation of ocular drugs into a difficult task with a low rate of therapeutic response. One of the challenges for the new generation of ophthalmic pharmaceutical formulations is to increase the bioavailability of drugs administered by the ocular route and, therefore, their therapeutic efficacy. This can be achieved with the use of some strategies that provide an increase in the formulation pre-corneal residence time, mucoadhesion and penetration across the eye tissues...
2014: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
Marcelo Leite Vieira Costa, Roberto César Pereira Lima-Júnior, Karoline Sabóia Aragão, Raul Pinheiro Medeiros, Raphael Dias Marques-Neto, Lucas de Sá Grassi, Leandro Linhares Leite, Lorena Guimaraes Nunes, José Wilson Benevides de Mesquita Neto, Gerly Anne de Castro Brito, Marcellus Henrique Loiola Pontes de Souza, Paulo Roberto Carvalho de Almeida, Ronaldo Albuquerque Ribeiro
PURPOSE: Nonalcoholic steatohepatitis (NASH) has been associated with irinotecan (IRI)-based cancer chemotherapy regimens. The purpose of this study was to propose and test a consistent model of IRI-induced NASH, filling a gap in the medical literature. METHODS: Swiss male mice were distributed in groups (n = 8) and injected with saline (5 mL/kg, i.p.; control) or IRI (25, 50, 75 or 100 mg/kg, i.p.) thrice a week for 7 weeks. Blood samples were collected to measure the serum concentrations of proteins, alanine and aspartate aminotransferases (ALT and AST)...
October 2014: Cancer Chemotherapy and Pharmacology
Ribrio Ivan Tavares Pereira Batista, Nádia Rezende Barbosa Raposo, Paulo Henrique Almeida Campos-Junior, Michele Munk Pereira, Luiz Sergio Almeida Camargo, Bruno Campos Carvalho, Marco Antonio Sundfeld Gama, João Henrique Moreira Viana
BACKGROUND: Due to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal calf serum...
2014: Journal of Animal Science and Biotechnology
Letícia Siqueira de Sá Barretto, Camila Lessio, Ahy Natally Sawaki e Nakamura, Edson Guimarães Lo Turco, Camila Gonzaga da Silva, João Paulo Zambon, Fábio César Gozzo, Eduardo Jorge Pilau, Fernando Gonçalves de Almeida
Human adipose tissue has been described as a potential alternative reservoir for stem cells. Although studies have been performed in rabbits using autologous adipose-derived stem cells (ADSC), these cells have not been well characterized. The primary objectives of this study were to demonstrate the presence of adipose-derived stem cells isolated from rabbit inguinal fat pads and to characterize them through osteogenic and adipogenic in vitro differentiation and lipid fingerprinting analysis. The secondary objective was to evaluate cell behavior through growth kinetics, cell viability, and DNA integrity...
October 2014: In Vitro Cellular & Developmental Biology. Animal
Tiemy Rosana Komatsu, Simone Kimie Oku, Suely Godoy Agostinho Gimeno, Leiko Asakura, Luciola de Castro Coelho, Clarissa Viana Demezio da Silva, Rita de Cassia Coelho Almeida Akutsu, Anita Sachs
A validity test of a Food Frequency Questionnaire was carried out using 50 students of health occupation in São Paulo, Brazil. Therefore, a three day dietary record was used as reference method and variables such as energy, macronutrients and dietary fiber were analyzed. The accordance between the Food Frequency Questionnaire and average data from dietary record was tested with kappa statistics and intra-class correlation coefficients (ICC). Limits of agreement were estimated by the Bland-Altman's method. Better results were found for calories (ICC 0...
December 2013: Revista Brasileira de Epidemiologia, Brazilian Journal of Epidemiology
Célia Landmann Szwarcwald, Deborah Carvalho Malta, Cimar Azeredo Pereira, Maria Lucia França Pontes Vieira, Wolney Lisboa Conde, Paulo Roberto Borges de Souza Júnior, Giseli Nogueira Damacena, Luiz Otávio Azevedo, Gulnar Azevedo E Silva, Mariza Miranda Theme Filha, Cláudia de Souza Lopes, Dália Elena Romero, Wanessa da Silva de Almeida, Carlos Augusto Monteiro
The National Health Survey is a household-based nationwide survey carried out by the Ministry of Health in partnership with the Brazilian Institute of Geography and Statistics. The scope of the survey is to establish the health status and lifestyles of the population - as well as how they look after their health - with regard to access and use of services, preventive actions, continuity of care, and health care financing. The sample size is 80,000 households and enables the calculation of some indicators at different geographic levels, namely states, capitals, metropolitan and rural areas...
February 2014: Ciência & Saúde Coletiva
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