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Almeida lipid

Francis D O Ablan, B Logan Spaller, Kaitlyn I Abdo, Paulo F Almeida
Hundreds of cationic antimicrobial and cell-penetrating peptides (CPPs) form amphipathic α-helices when bound to lipid membranes. Here, we test two hypotheses for the differences in the ability of these peptides to translocate across membranes. The first, which we now call the hydrophobicity hypothesis, is that peptide translocation is determined by the Gibbs energy of insertion into the bilayer from the membrane interface. The second, which we call the charge-distribution hypothesis, is that translocation is determined by whether the distribution of cationic residues in the peptide can transiently stabilize a high-energy inserted intermediate by forming salt bridges to the phosphates of lipid headgroups...
October 18, 2016: Biophysical Journal
Camila Henriques Coelho, Adriana Oliveira Costa, Ana Carolina Carvalho Silva, Maíra Mazzoni Pucci, Angela Vieira Serufo, Haendel Goncalves Nogueira Oliveira Busatti, Maurício Durigan, Jonas Perales, Alex Chapeaurouge, Daniel Almeida da Silva E Silva, Maria Aparecida Gomes, Juliano Simões Toledo, Steven M Singer, Rosiane A Silva-Pereira, Ana Paula Fernandes
The zoonotic potential of giardiasis, as proposed by WHO since the late 70's, has been largely confirmed in this century. The genetic assemblages A and B of Giardia duodenalis are frequently isolated from human and canine hosts. Most of the assemblage A strains are not infective to adult mice, which can limit the range of studies regarding to biology of G. duodenalis, including virulence factors and the interaction with host immune system. This study aimed to determine the infectivity in mice of an assemblage A Giardia duodenalis strain (BHFC1) isolated from a dog and to classify the strain in sub-assemblages (AI, AII, AIII) through the phylogenetic analysis of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes...
2016: PloS One
Mariah J King, Ashley L Bennett, Paulo F Almeida, Hee-Seung Lee
δ-lysin, secreted by a Gram-positive bacterium Staphylococcus aureus, is a 26-residue membrane active peptide that shares many common features with antimicrobial peptides (AMPs). However, it possesses a few unique features that differentiate itself from typical AMPs. In particular, δ-lysin has zero net charge, even though it has many charged residues, and it preferentially lyses eukaryotic cells over bacterial cells. Here, we present the results of coarse-grained molecular dynamics simulations of δ-lysin interacting with a zwitterionic membrane over a wide range of peptide concentrations...
October 6, 2016: Biochimica et Biophysica Acta
Cássia R A da Cunha, Luís C N da Silva, Fábio J F Almeida, Milena S Ferraz, Nathalia Varejão, Marina F de Souza Cartaxo, Rita de Cássia M de Miranda, Francisco C A de Aguiar, Noemia P da Silva Santos, Luana C B B Coelho, Nereide S Santos-Magalhães, Maria T Dos Santos Correia
This study evaluated the in vivo antitumor potential of the recombinant lectin from seeds of Cratylia mollis (rCramoll) expressed in Escherichia coli, free or encapsulated in stealth liposomes, using mice transplanted with sarcoma 180. rCramoll-loaded stealth liposomes (rCramoll-lipo) were formulated by hydration of the lipid film followed by cycles of freezing and thawing, and about 60% of rCramoll was encapsulated. This novel preparation showed particle size, polydispersity index, and pH suitable for the evaluation of antitumor activity in vivo...
2016: Frontiers in Microbiology
Ignacio Jofré, Cesar Pezoa, Magdalena Cuevas, Erick Scheuermann, Irlan Almeida Freires, Pedro Luiz Rosalen, Severino Matias de Alencar, Fernando Romero
Hypertension is a systemic condition with high morbidity and mortality rates worldwide, which poses an increased risk for cardiovascular diseases. In this study, we demonstrated the antioxidant and vasodilator activity of Ugni molinae Turcz. (Murtilla) fruit, a berry native to Chile and proposed models to explain its modulatory mechanism in hypotensive response. Murtilla fruits were cultivated in a germplasm bank and submitted to chemical and biological analyses. The phenolic compounds gallic acid, Catechin, Quercetin-3-β-D-glucoside, Myricetin, Quercetin, and Kaempferol were identified...
2016: Oxidative Medicine and Cellular Longevity
Kirstin Aschbacher, Jeffrey M Milush, Amanda Gilbert, Carlos Almeida, Elizabeth Sinclair, Lorrie Epling, S Marlene Grenon, Elysa J Marco, Eli Puterman, Elissa Epel
BACKGROUND: Chronic psychological stress is a risk factor for cardiovascular disease and mortality. Circulating hematopoietic progenitor cells (CPCs) maintain vascular homeostasis, correlate with preclinical atherosclerosis, and prospectively predict cardiovascular events. We hypothesize that 1) chronic caregiving stress is related to reduced CPC number, and 2) this may be explained in part by negative interactions within the family. METHODS: We investigated levels of stress and CPCs in 68 healthy mothers - 31 of these had children with an autism spectrum disorder (M-ASD) and 37 had neurotypical children (M-NT)...
September 10, 2016: Brain, Behavior, and Immunity
Xueyun Zheng, Xing Zhang, Nathaniel S Schocker, Ryan S Renslow, Daniel J Orton, Jamal Khamsi, Roger A Ashmus, Igor C Almeida, Keqi Tang, Catherine E Costello, Richard D Smith, Katja Michael, Erin S Baker
Glycomics has become an increasingly important field of research since glycans play critical roles in biology processes ranging from molecular recognition and signaling to cellular communication. Glycans often conjugate with other biomolecules, such as proteins and lipids, and alter their properties and functions, so glycan characterization is essential for understanding the effects they have on cellular systems. However, the analysis of glycans is extremely difficult due to their complexity and structural diversity (i...
September 7, 2016: Analytical and Bioanalytical Chemistry
Bianca de Almeida-Pititto, Fernando Flexa Ribeiro-Filho, Sandhi Barreto, Bruce B Duncan, Maria Inês Schmidt, Paulo A Lotufo, Isabela M Bensenor, Sandra R G Ferreira
Objective: Our aim was to describe the distribution of selected biomarkers according to age and sex, adjusted for HOMA-IR and adiposity, in a subset of middle-aged individuals of Brazilian Longitudinal Study of Adult Health-ELSA without diabetes mellitus or CVD. Subjects and methods: This cross-sectional study was conducted in 998 participants of the ELSA-Brasil without diabetes and/or cardiovascular disease. In addition to the traditional risk factors, several biomarkers concentrations were compared according to sex, age groups (35-44; 45-54 yrs) and HOMA-IR tertiles...
September 5, 2016: Archives of Endocrinology and Metabolism
Luciana Gavilan Dias Folchetti, Isis Tande da Silva, Bianca de Almeida-Pititto, Sandra Roberta G Ferreira
INTRODUCTION: Non-communicable chronic diseases (NCCDs) represent a burden for public health. Alongside the established cardiometabolic risk factors such as high blood pressure and disorders of glucose and lipid metabolism, living habits and nutritional status at different stages of life are seen as contributors to this scenario. Gut microbiota composition and subclinical inflammation have been pointed out as underlying mechanisms of NCCDs. Studies involving health professionals have brought relevant contributions to the knowledge about risk factors...
2016: BMJ Open
Bianca de Almeida-Pititto, Fernando Flexa Ribeiro-Filho, Itamar S Santos, Paulo A Lotufo, Isabela M Bensenor, Sandra Rg Ferreira
OBJECTIVE: The study assessed the association of adiponectin concentration with carotid intima-media thickness (CIMT) in middle-aged participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) without diabetes or cardiovascular disease. DESIGN: Cross-sectional analyses. METHODS: A sample of 687 individuals (35-54 years old) without diabetes or cardiovascular disease was stratified into two categories according to CIMT (< or ≥ 75th percentile)...
August 22, 2016: European Journal of Preventive Cardiology
Vinicius C Soeiro, Karoline R T Melo, Monique G C F Alves, Mayara J C Medeiros, Maria L P M Grilo, Jailma Almeida-Lima, Daniel L Pontes, Leandro S Costa, Hugo A O Rocha
Dextrans (α-d-glucans) extracted from Leuconostoc mesenteroides, with molecular weights (MW) of 10 (D10), 40 (D40) and 147 (D147) kDa, were evaluated as antioxidant, anticoagulant and immunomodulatory drugs for the first time. None presented anticoagulant activity. As for the antioxidant and immunomodulatory tests, a specific test showed an increase in the dextran activity that was proportional to the increase in molecular weight. In a different assay, however, activity decreased or showed no correlation to the MW...
2016: International Journal of Molecular Sciences
Aline Raquel Voltan, Guillermo Quindós, Kaila P Medina Alarcón, Ana Marisa Fusco-Almeida, Maria José Soares Mendes-Giannini, Marlus Chorilli
Invasive mycoses are a major problem for immunocompromised individuals and patients in intensive care units. Morbidity and mortality rates of these infections are high because of late diagnosis and delayed treatment. Moreover, the number of available antifungal agents is low, and there are problems with toxicity and resistance. Alternatives for treating invasive fungal infections are necessary. Nanostructured systems could be excellent carriers for antifungal drugs, reducing toxicity and targeting their action...
2016: International Journal of Nanomedicine
Wellison Jarles da Silva Diniz, Luiz Lehmann Coutinho, Polyana Cristine Tizioto, Aline Silva Mello Cesar, Caio Fernando Gromboni, Ana Rita Araújo Nogueira, Priscila Silva Neubern de Oliveira, Marcela Maria de Souza, Luciana Correia de Almeida Regitano
Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter...
2016: PloS One
Michael R Kanost, Estela L Arrese, Xiaolong Cao, Yun-Ru Chen, Sanjay Chellapilla, Marian R Goldsmith, Ewald Grosse-Wilde, David G Heckel, Nicolae Herndon, Haobo Jiang, Alexie Papanicolaou, Jiaxin Qu, Jose L Soulages, Heiko Vogel, James Walters, Robert M Waterhouse, Seung-Joon Ahn, Francisca C Almeida, Chunju An, Peshtewani Aqrawi, Anne Bretschneider, William B Bryant, Sascha Bucks, Hsu Chao, Germain Chevignon, Jayne M Christen, David F Clarke, Neal T Dittmer, Laura C F Ferguson, Spyridoula Garavelou, Karl H J Gordon, Ramesh T Gunaratna, Yi Han, Frank Hauser, Yan He, Hanna Heidel-Fischer, Ariana Hirsh, Yingxia Hu, Hongbo Jiang, Divya Kalra, Christian Klinner, Christopher König, Christie Kovar, Ashley R Kroll, Suyog S Kuwar, Sandy L Lee, Rüdiger Lehman, Kai Li, Zhaofei Li, Hanquan Liang, Shanna Lovelace, Zhiqiang Lu, Jennifer H Mansfield, Kyle J McCulloch, Tittu Mathew, Brian Morton, Donna M Muzny, David Neunemann, Fiona Ongeri, Yannick Pauchet, Ling-Ling Pu, Ioannis Pyrousis, Xiang-Jun Rao, Amanda Redding, Charles Roesel, Alejandro Sanchez-Gracia, Sarah Schaack, Aditi Shukla, Guillaume Tetreau, Yang Wang, Guang-Hua Xiong, Walther Traut, Tom K Walsh, Kim C Worley, Di Wu, Wenbi Wu, Yuan-Qing Wu, Xiufeng Zhang, Zhen Zou, Hannah Zucker, Adriana D Briscoe, Thorsten Burmester, Rollie J Clem, René Feyereisen, Cornelis J P Grimmelikhuijzen, Stavros J Hamodrakas, Bill S Hansson, Elisabeth Huguet, Lars S Jermiin, Que Lan, Herman K Lehman, Marce Lorenzen, Hans Merzendorfer, Ioannis Michalopoulos, David B Morton, Subbaratnam Muthukrishnan, John G Oakeshott, Will Palmer, Yoonseong Park, A Lorena Passarelli, Julio Rozas, Lawrence M Schwartz, Wendy Smith, Agnes Southgate, Andreas Vilcinskas, Richard Vogt, Ping Wang, John Werren, Xiao-Qiang Yu, Jing-Jiang Zhou, Susan J Brown, Steven E Scherer, Stephen Richards, Gary W Blissard
Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M...
September 2016: Insect Biochemistry and Molecular Biology
Luis Felipe das Chagas E Silva, Thiago Martini Pereira, Taciana Depra Magrini, Ana Sueli Rodrigues Cavalcante, Herculano da Silva Martinho, Janete Dias Almeida
: Actinic cheilitis (AC) is considered a potentially malignant disorder of the lip. Biomolecular markers study is important to understand malignant transformation into squamous cell carcinoma. Fourier transform infra red (FT-IR) spectroscopy was used to analyze AC in this study. OBJECTIVES: The aim of the study was to evaluate if FT-IR spectral regions of nucleic acids and collagen can help in early diagnosis of malignant transformation. METHODS: Tissues biopsies of 14 patients diagnosed with AC and 14 normal tissues were obtained...
August 1, 2016: Photodiagnosis and Photodynamic Therapy
Daniel A M Toledo, Natália R Roque, Lívia Teixeira, Erix A Milán-Garcés, Alan B Carneiro, Mariana R Almeida, Gustavo F S Andrade, Jefferson S Martins, Roberto R Pinho, Célio G Freire-de-Lima, Patrícia T Bozza, Heloisa D'Avila, Rossana C N Melo
Most eukaryotic cells contain varying amounts of cytosolic lipidic inclusions termed lipid bodies (LBs) or lipid droplets (LDs). In mammalian cells, such as macrophages, these lipid-rich organelles are formed in response to host-pathogen interaction during infectious diseases and are sites for biosynthesis of arachidonic acid (AA)-derived inflammatory mediators (eicosanoids). Less clear are the functions of LBs in pathogenic lower eukaryotes. In this study, we demonstrated that LBs, visualized by light microscopy with different probes and transmission electron microscopy (TEM), are produced in trypomastigote forms of the parasite Trypanosoma cruzi, the causal agent of Chagas' disease, after both host interaction and exogenous AA stimulation...
2016: PloS One
Claudia Almeida, Antonin Lamazière, Angélique Filleau, Yohann Corvis, Philippe Espeau, Jesus Ayala-Sanmartin
Cell penetrating peptides are promising vectors for molecular drug delivery in eukaryotic cells. Despite of their discovery 20years ago, the mechanisms of peptide membrane crossing are still controversial. The different suggested penetration mechanisms reflect the high sequence and structural diversity of cell penetrating peptides. The fundamental step for peptide penetration into the cytosol is the crossing of the membrane lipid barrier at the level of the plasma membrane or the endosomes. Therefore, the study of the peptide-lipid interaction is the key for peptide penetration mechanisms understanding...
July 27, 2016: Biochimica et Biophysica Acta
Brunno F R Caetano, Nelci A de Moura, Ana P S Almeida, Marcos C Dias, Kátia Sivieri, Luís F Barbisan
Yacon (Smallanthus sonchifolius), a perennial plant of the family Asteraceae native to the Andean regions of South America, is an abundant source of fructooligosaccharides (FOS). This comprehensive review of the literature addressed the role of yacon supplementation in promoting health and reducing the risk of chronic diseases. According to several preclinical and clinical trials, FOS intake favors the growth of health-promoting bacteria while reducing pathogenic bacteria populations. Moreover, the endproducts of FOS fermentation by the intestinal microbiota, short chain fatty acids (SCFA), act as substrates or signaling molecules in the regulation of the immune response, glucose homeostasis and lipid metabolism...
2016: Nutrients
Itahisa Marcelino Rodríguez, José Oliva García, José Juan Alemán Sánchez, Delia Almeida González, Santiago Domínguez Coello, Buenaventura Brito Díaz, Fadoua Gannar, María Del Cristo Rodríguez Pérez, Roberto Elosua, Antonio Cabrera de León
AIMS: To analyze the serum lipid and inflammatory biomarker profile in the early insulin resistance (e-IR). METHODS: Cross-sectional study of 5943 adults without diabetes, stratified into no IR group (C-peptide <third tertile and glucose <100 mg/dL), e-IR group (C-peptide ≥third tertile and glucose <100 mg/dL) and advanced IR group (glucose ≥100 mg/dL). RESULTS: E-IR showed significant differences with no IR in the serum concentration of triglycerides (P < 0...
July 18, 2016: Acta Diabetologica
Helen Sadauskas-Henrique, Susana Braz-Mota, Rafael Mendonça Duarte, Vera Maria Fonseca de Almeida-Val
The increment in crude oil exploitation over the last decades has considerably increased the risk of polycyclic aromatic hydrocarbon (PAH) contamination to Amazonian aquatic environments, especially for the black water environments such as the Rio Negro. The present work was designed to evaluate the acute toxicity of the Urucu crude oil (CO), the chemically dispersed Urucu crude oil (CO + D), and the dispersant alone (D) to the Amazonian fish Colossoma macropomum. Acute toxicity tests were performed, using a more realistic approach, where fish were acclimated to both groundwater (GW), used as internal control, and natural Rio Negro water (RNW) and exposed to CO, CO + D and D...
October 2016: Environmental Science and Pollution Research International
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