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https://www.readbyqxmd.com/read/28631525/polyphenol-profile-by-uhplc-ms-ms-anti-glycation-antioxidant-and-cytotoxic-activities-of-several-samples-of-propolis-from-the-northeastern-semi-arid-region-of-brazil
#1
Jadriane de Almeida Xavier, Iara Barros Valentim, Fabiana O S Camatari, Alberto M M de Almeida, Henrique Fonseca Goulart, Jamylle Nunes de Souza Ferro, Emiliano de Oliveira Barreto, Bruno Coelho Cavalcanti, Carla B G Bottoli, Marília Oliveira Fonseca Goulart
CONTEXT: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. OBJECTIVE: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. MATERIAL AND METHODS: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH(•), FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28603929/lipid-like-peptides-can-stabilize-integral-membrane-proteins-for-biophysical-and-structural-studies
#2
Katharina Veith, Maria Martinez Molledo, Yasser Almeida Hernandez, Inokentijs Josts, Julius Nitsche, Christian Löw, Henning Tidow
A crucial bottleneck in membrane protein structural biology is the difficulty in identifying a detergent that can maintain the stability and functionality of integral membrane proteins (IMPs). Detergents are poor membrane mimics and their common use in membrane protein crystallography may be one reason for the challenges in obtaining high-resolution crystal structures of many IMP families. Lipid-like peptides (LLPs) have detergent-like properties and have been proposed as alternatives for the solubilization of GPCRs and other membrane proteins...
June 11, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28599665/omega-3-supplementation-on-inflammatory-markers-in-patients-with-chronic-chagas-cardiomyopathy-a-randomized-clinical-study
#3
Paula Simplício da Silva, Mauro Felippe Felix Mediano, Gilberto Marcelo Sperandio da Silva, Patricia Dias de Brito, Claudia Santos de Aguiar Cardoso, Cristiane Fonseca de Almeida, Luiz Henrique Conde Sangenis, Roberta Olmo Pinheiro, Alejandro Marcel Hasslocher-Moreno, Pedro Emmanuel Alvarenga Americano do Brasil, Andrea Silvestre de Sousa
BACKGROUND: Several studies have been focusing on the effect of omega-3 polyunsaturated fatty acids on modulation of inflammatory markers in several cardiopathies. Although immunoregulatory dysfunction has been associated to the chronic cardiac involvement in Chagas disease, there is no study examining the effects of omega-3 supplementation in these patients. We investigated the effects of omega-3 PUFAs on markers of inflammation and lipid profile in chronic Chagas cardiomyopathy patients...
June 9, 2017: Nutrition Journal
https://www.readbyqxmd.com/read/28582701/oxidative-stress-in-the-algae-chlamydomonas-reinhardtii-exposed-to-biocides
#4
Ana Catarina Almeida, Tânia Gomes, Katherine Langford, Kevin V Thomas, Knut Erik Tollefsen
The toxicity of biocides can be associated with the formation of reactive oxygen species (ROS) and subsequent oxidative damage, interfering with the normal function of photosynthetic organisms. This study investigated the formation and effects of ROS in the unicellular green algae Chlamydomonas reinhardtii exposed to three environmentally relevant biocides, aclonifen, dichlofluanid and triclosan. After a first screening to identify which biocides induced ROS, a 24h multi-endpoint analysis was used to verify the possible consequences...
May 31, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28578237/ecotoxicity-of-the-antihistaminic-drug-cetirizine-to-ruditapes-philippinarum-clams
#5
Ângela Almeida, Vânia Calisto, Valdemar I Esteves, Rudolf J Schneider, Amadeu M V M Soares, Etelvina Figueira, Rosa Freitas
Cetirizine (CTZ) is an antihistaminic drug present in the aquatic environment, with limited information on its toxicity to organisms inhabiting this system. This study intended to evaluate the effects of CTZ on oxidative stress and energy metabolism biomarkers in the edible clam Ruditapes philippinarum after a 28days exposure to environmentally relevant CTZ concentrations (0.0, 0.3, 3.0, 6.0 and 12.0μg/L). The results obtained showed that CTZ was accumulated by clams reaching maximum concentrations (up to ~22ng/g FW) at the highest CTZ exposure concentrations (6...
June 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28571587/supplementation-of-grazing-beef-cows-during-gestation-as-a-strategy-to-improve-skeletal-muscle-development-of-the-offspring
#6
D C Marquez, M F Paulino, L N Rennó, F C Villadiego, R M Ortega, D S Moreno, L S Martins, D M de Almeida, M P Gionbelli, M R Manso, L P Melo, F H Moura, M S Duarte
The appropriate supply of nutrients in pregnant cows has been associated with the optimal development of foetal tissues, performance of their progeny and their meat quality. The aim of this study was to evaluate supplementation effects of grazing cows in different stages of gestation on skeletal muscle development and performance of the progeny. Thereby, 27 Nellore cows were divided into three groups (n=9 for each group) and their progeny as follows: UNS, unsupplemented during gestation; MID, supplemented from 30 to 180 days of gestation; LATE, supplemented from 181 to 281 days of gestation...
June 2, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28567440/brazilian-morus-nigra-attenuated-hyperglycemia-dyslipidemia-and-prooxidant-status-in-alloxan-induced-diabetic-rats
#7
Ivanildo I da S Júnior, Humberto de Moura Barbosa, Débora C R Carvalho, Ruideglan de Alencar Barros, Flávia Peixoto Albuquerque, Dionísio Henrique Amaral da Silva, Grasielly R Souza, Nathália A C Souza, Larissa A Rolim, Flaviane M M Silva, Glória I B P Duarte, Jackson R G da S Almeida, Flávio Monteiro de Oliveira Júnior, Dayane A Gomes, Eduardo C Lira
Morus nigra has been used popularly for several proposes, including diabetic. In an attempt to support medicinal value, the acute hypoglycemic, hypolipidemic, and antioxidant effects of the ethanolic extract of Morus nigra (EEMn 200 or 400 mg/kg b.w.) were evaluated in normal and alloxan-induced diabetic treated for 14 days. Serum biochemical and antioxidant analysis were performed at the end of experiment. Oral glucose tolerance test was performed at 10th and 15th days. Chromatographic analysis by HPLC-DAD of EEMn was performed...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28559960/medicinal-plants-for-the-treatment-of-obesity-ethnopharmacological-approach-and-chemical-and-biological-studies
#8
REVIEW
Luciano Mamede de Freitas Junior, Eduardo B de Almeida
Obesity is a global epidemic that has shown a steady increase in morbimortality indicators; it is considered a social problem and entails serious health risks. One of the alternatives in the treatment of obesity is the traditional use of medicinal plants, which supports the research and development of obesity phytotherapy. In this article, we provide information about ethnopharmacological species used to treat obesity, through an electronic search of the periodical databases Web of Science, Scopus, PubMed and Scielo, considering the period 1996-2015 and using the descriptors "plants for obesity", "ethnopharmacology for obesity" and "anti-obesity plants" in both Portuguese and English...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28513635/the-impact-of-dark-chocolate-intake-on-arterial-elasticity-in-individuals-with-hiv-aids-undergoing-art-a-randomized-double-blind-crossover-trial
#9
Andrea Mariana Nunes da Costa Teixeira, Liania Alves Luzia, Suelen Jorge de Souza, Aline de Almeida Petrilli, Patrícia de Moraes Pontilho, Jose Maria Pacheco de Souza, Aluísio Augusto Cotrim Segurado, Priscila Efraim, Camila de Melo Picone, Patrícia Helen de Carvalho Rondo
An increase in the frequency of cardiovascular diseases has been observed in the HIV/AIDS population. Studies involving healthy subjects or subjects with other diseases have shown benefits of chocolate supplementation on endothelial function and vasodilation. We evaluate the impact of chocolate consumption on arterial elasticity in people living with human immunodeficiency virus - PLHIV. A double-blind, crossover trial including 110 PLHIV (19 to 59 years) on antiretroviral therapy - ART for at least 6 months and with a viral load of <500 copies per mL was conducted...
May 17, 2017: Food & Function
https://www.readbyqxmd.com/read/28504003/different-metabolic-responses-induced-by-long-term-interdisciplinary-therapy-in-obese-adolescents-related-to-ace-i-d-polymorphism
#10
Sandro S Almeida, Flavia C Corgosinho, Carlos En Amorim, Marcos F Gregnani, Raquel Ms Campos, Deborah Cl Masquio, Priscila L Sanches, Aline P Ganen, João B Pesquero, Ana R Dâmaso, Marco T Mello, Sergio Tufik, Ronaldo C Araújo
INTRODUCTION: The main purpose of the present study was to investigate whether I/D polymorphism of the ACE gene might affect metabolic changes related to the metabolic syndrome through a long-term interdisciplinary therapy in obese adolescents. METHODS: In total, 125 obese adolescents who entered the interdisciplinary obesity programme were assigned to the following two subgroups: metabolic syndrome or non-metabolic syndrome. They were evaluated at baseline and after 1 year...
April 2017: Journal of the Renin-angiotensin-aldosterone System: JRAAS
https://www.readbyqxmd.com/read/28489128/obesity-bariatric-surgery-and-oxidative-stress
#11
Roberta Cattaneo Horn, Gabriela Tassotti Gelatti, Natacha Cossettin Mori, Ana Caroline Tissiani, Mariana Spanamberg Mayer, Elvio Almeida Pereira, Marcelo Ross, Paulo Ricardo Moreira, Josiane Woutheres Bortolotto, Tamiris Felippin
Introduction: Obesity refers to the accumulation of fatty tissues and it favors the occurrence of oxidative stress. Alternatives that can contribute to body weight reduction have been investigated in order to reduce the production of reactive oxygen species responsible for tissue damage. The aim of the current study was to assess whether the oxidant and antioxidant markers of obese women before and after bariatric surgery were able to reduce oxidative damage. Method: We have assessed 16 morbidly obese women five days before and 180 days after the surgery...
March 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28457947/influence-of-temperature-on-the-antioxidant-responses-and-lipid-peroxidation-of-two-species-of-tadpoles-rhinella-schneideri-and-physalaemus-nattereri-exposed-to-the-herbicide-sulfentrazone-boral-500sc%C3%A2
#12
Juliane Silberschmidt Freitas, Fabrício Barreto Teresa, Eduardo Alves de Almeida
Amphibians can experience large temperature fluctuations in their habitats, especially during the larval stage, when tadpoles are restricted to small and ephemeral ponds. Changes in water temperature can alter development, metabolism and behaviour of cold-blooded animals but also the toxicokinetics of chemicals in the environment. In Brazil, pesticides application is intensified during the rainy season, which is the period of reproduction for many amphibian species. We evaluated here the influence of temperature (28, 32, and 36°C) on the toxicity of the herbicide sulfentrazone (Boral®SC) in tadpoles of Physalaemus nattereri and Rhinella schneideri, by analysis of oxidative stress biomarkers...
April 27, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28454924/dual-effect-of-silymarin-on-experimental-non-alcoholic-steatohepatitis-induced-by-irinotecan
#13
Eudmar Marcolino Assis-Júnior, Anielle Torres Melo, Venúcia Bruna Magalhães Pereira, Deysi Viviana Tenazoa Wong, Nathalia Ribeiro Pinho Sousa, Christiane Mendes Gonçalves Oliveira, Lara Raissa Cavalcante Malveira, Leonardo Silva Moreira, Marcellus Henrique Loiola Ponte Souza, Paulo Roberto Carvalho Almeida, Roberto César Pereira Lima-Júnior
Irinotecan-based regimens are commonly used for treatment of colorectal cancer, which is limited by mucositis and non-alcoholic steatohepatitis (NASH). Silymarin (SIL) prevents fatty liver disease in the clinical setting and in models of liver damage induced chemically. This study investigated the possible effect of SIL on irinotecan (IRI)-induced NASH. Swiss female mice were injected with saline (SAL 5ml/kg i.p.), IRI (50mg/kg i.p.), SIL (150mg/kg p.o.) or IRI (50mg/kg i.p.)+(SIL 1.5, 15 or 150mg/kg p.o.) thrice/week/7weeks...
July 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28442267/shell-alterations-in-limpets-as-putative-biomarkers-for-multi-impacted-coastal-areas
#14
Felipe Nincao Begliomini, Daniele Claudino Maciel, Sérgio Mendonça de Almeida, Denis Moledo Abessa, Luciane Alves Maranho, Camilo Seabra Pereira, Gilvan Takeshi Yogui, Eliete Zanardi-Lamardo, Ítalo Braga Castro
During the last years, shell alterations in gastropods have been proposed as tools to be used in monitoring programs. However, no studies were so far performed investigating the relationships among shell parameters and classical biomarkers of damage. The relationship between shell alterations (biometrics, shape and elemental composition) and biomarkers (LPO and DNA strand break) was evaluated in the limpet L. subrugosa sampled along a contamination gradient in a multi-impacted coastal zone from southeastern Brazil...
April 22, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28431322/xanthohumol-and-8-prenylnaringenin-ameliorate-diabetic-related-metabolic-dysfunctions-in-mice
#15
Raquel Costa, Ilda Rodrigues, Luísa Guardão, Sílvia Rocha-Rodrigues, Carolina Silva, José Magalhães, Manuel Ferreira-de-Almeida, Rita Negrão, Raquel Soares
Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by metabolic disturbances in specific tissues. The present work aimed to analyze the effects of xanthohumol (XN) and 8-prenylnaringenin (8PN), two beer-derived polyphenols, in liver and skeletal muscle lipid and glycolytic metabolism in T2DM mice model. Thirty C57Bl/6 mice were randomly divided into five groups: standard diet (control), high-fat diet (DM), high-fat diet plus ethanol (DM-Ethanol), high-fat diet plus 10 mg/L XN (DM-XN) and high-fat diet plus 10 mg/L 8PN (DM-8PN) during 20 weeks...
April 6, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28429194/photobiomodulation-therapy-associated-with-treadmill-training-in-the-oxidative-stress-in-a-collagen-induced-arthritis-model
#16
Solange Almeida Dos Santos, Marcia Ataize Dos Santos Vieira, Maira Cécilia Brandão Simões, Andrey Jorge Serra, Ernesto Cesar Leal-Junior, Paulo de Tarso Camillo de Carvalho
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by chronic and systemic inflammation, which leads to the destruction of the cartilage and bone and affects tissues in multiple joints. Oxidative stress has been implicated with regards to involvement in various disease conditions, such as diabetes mellitus and neurodegenerative, respiratory, cardiovascular, and RA diseases. In vivo experimental studies using photobiomodulation therapy (PBMT) have shown positive effects in reducing lipid peroxidation and in increasing antioxidant activity...
April 21, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28402322/nutritional-status-in-patients-with-phenylketonuria-using-glycomacropeptide-as-their-major-protein-source
#17
A Pinto, M F Almeida, P C Ramos, S Rocha, A Guimas, R Ribeiro, E Martins, A Bandeira, A MacDonald, J C Rocha
BACKGROUND/OBJECTIVES: Low phenylalanine (PHE), glycomacropeptide-based protein substitute (GMP) is an alternative to traditional L-amino acid supplements (AA) used in the dietary management of phenylketonuria (PKU). In a retrospective, longitudinal study, we report the nutritional status of PKU patients taking AA and GMP. SUBJECTS/METHODS: Eleven PKU patients aged 27±10 years (1 HPA, 4 mild and 6 classical PKU) on dietary treatment were evaluated (anthropometry, body composition, blood pressure measurements, biochemical markers including vitamin, mineral, lipids, carbohydrates and protein status/metabolism, and nutritional intake assessment) at two different annual reviews...
April 12, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28396017/cell-surface-targeting-of-the-melanocortin-5-receptor-mc5r-requires-serine-rich-terminal-motifs
#18
A R Rodrigues, D Sousa, H Almeida, A M Gouveia
The Melanocortin 5 Receptor (MC5R) is a cell surface receptor that belongs to the class of G-protein coupled receptors (GPCRs), which comprises an intracellular carboxylic domain, seven transmembrane helices and an extracellular amino terminal. Over the last few years, MC5R has been implicated in the regulation of lipid metabolism in exocrine glands, muscle and even in adipose tissue and its function is quite dependent on its correct cell membrane targeting. In this context, the purpose of this work was to study the role of MC5R N-terminus in the receptor trafficking from the endoplasmic reticulum (ER) through the Golgi complex to the plasma membrane...
April 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28395280/triple-a-syndrome-preliminary-response-to-the-antioxidant-n-acetylcysteine-treatment-in-a-child
#19
Maria Candida Barisson Villares Fragoso, Edoarda Vasco de Albuquerque Albuquerque, Ana Luiza de Almeida Cardoso, Paula Waki Lopes da Rosa, Rodrigo Bomeny de Paulo, Maria Heloisa Massola Schimizu, Antonio Carlos Seguro, Marisa Passarelli, Katrin Koehler, Angela Huebner, Madson Q Almeida, Ana Claudia Latronico, Ivo Jorge Prado Arnhold, Berenice Bilharinho Mendonca
INTRODUCTION: Triple A syndrome (AAAS) is a rare autosomal recessive disorder characterized by alacrima, achalasia, ACTH-resistant adrenal insufficiency, autonomic dysfunction, and progressive neurodegeneration. Increased oxidative stress, demonstrated in patients' fibroblasts in vitro, may be a central disease mechanism. N-acetylcysteine protects renal function in patients with kidney injuries associated with increased oxidative stress and improves viability of AAAS-knockdown adrenal cells in vitro...
April 10, 2017: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/28348154/mechanisms-of-vesicular-stomatitis-virus-inactivation-by-protoporphyrin-ix-zinc-protoporphyrin-ix-and-mesoporphyrin-ix
#20
Christine Cruz-Oliveira, Andreza F Almeida, João M Freire, Marjolly B Caruso, Maria A Morando, Vivian N S Ferreira, Iranaia Assunção-Miranda, Andre M O Gomes, Miguel A R B Castanho, Andrea T Da Poian
Virus resistance to antiviral therapies is an increasing concern that makes urgent the development of broad spectrum antiviral drugs. Targeting viral envelope, a component shared by a large number of viruses, emerges as a promising strategy to overcome this problem. Natural and synthetic porphyrins, due to their relative hydrophobicity and pro-oxidant character, are good candidates for antivirals' development. In the present work, we characterized the antiviral activity of protoprophyrin IX (PPIX), Zn-protoporphyrin IX (ZnPPIX) and mesoporphyrin IX (MPIX) against vesicular stomatitis virus (VSV), and evaluated the mechanisms involved in this activity...
March 27, 2017: Antimicrobial Agents and Chemotherapy
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