Hugo Fernandes, Alessandra Zonnari, Ricardo Abreu, Sezin Aday, Marta Barão, Inês Albino, Miguel Lino, Ana Branco, Cátia Seabra, Tânia Barata, Ermelindo C Leal, José Guilherme Tralhão, Lino Gonçalves, Alwin de Jong, Hendrika A B Peters, Margreet R de Vries, Paula da Costa Martins, Paul H A Quax, Lino Ferreira
Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro , we showed that miR-425-5p, one of the hits, was able to enhance EC survival and migration. In vivo , using a mouse Matrigel plug assay, we showed that ECs transfected with miR-425-5p displayed enhanced survival compared with scramble-transfected ECs...
June 14, 2022: Molecular Therapy. Nucleic Acids