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Li Pan, Hongli Yang, Cong Xu, Shuangfeng Chen, Zhen Meng, Keyi Li, Haiying Chen
OBJECTIVE: Squamous cell carcinoma is often associated with the deletion or mutation of zinc finger protein 750 (ZNF750), its deletion or mutation is associated with squamous epithelial malignant biological characteristics. The present study is to explore the mechanism of ZNF750 to suppress the tumor malignant process by regulation tumor microenvironment. METHODS: To evaluate the changes of tumor microenvironment in oral squamous cells carcinoma cell line CAL-27 cell, the expression of angiogenin, vascular endothelial growth factor (VEGF), prolyl hydroxylase 2 (PHD2), G protein signal regulated protein 5 (RGS5), integrin A5 (ITGA5), integrin B1 (ITGB1) and CD44 were detected by Western-blot...
June 7, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hua-Sheng Ding, Yan Huang, Zhen Chen, Yan-Hong Tang, Dan-Dan Wang, Di Fan, Cong-Xin Huang
Regulator of G-protein signalling 5 (RGS5) is, highly expressed in different cell types of the adult human heart, and it is a negative regulator of G protein-mediated signalling that inactivates Gα(q) and Gα(i) and thereby inhibits many signalling pathways. However, the critical role of RGS5 in the pathology of myocardial infarction (MI) remains unexplored. Here, an in vitro MI model, induced by the permanent ligation of the left anterior descending coronary artery, was used with the isolated hearts of wild type (WT) and RGS5-knockout (KO) mice...
May 5, 2018: Biochemical and Biophysical Research Communications
Yumi Umeno, Sachiko Ogasawara, Jun Akiba, Satoshi Hattori, Hironori Kusano, Osamu Nakashima, Hironori Koga, Takuji Torimura, Ryoji Yamakawa, Hirohisa Yano
Portal vein invasion (PVI) is a major prognostic factor in hepatocellular carcinoma (HCC). The aim of the present study was to identify molecules that regulate PVI. Sections of cancerous tissue, paired noncancerous tissue and the PVI area were collected from 3 frozen HCC sections, using laser microdissection. The present study focused on 3 upregulated molecules, integrin β3 (ITGB3), secreted phosphoprotein 1 (SPP1) and regulator of G-protein signaling 5 (RGS5), and 2 molecules that were downregulated in PVI tissue compared with cancer tissue, metallothionein 1G (MT1G) and metallothionein 1H (MT1H), as determined by cDNA microarray analysis...
February 2018: Oncology Letters
Caroline Arnold, Eda Demirel, Anja Feldner, Guillem Genové, Hangjun Zhang, Carsten Sticht, Thomas Wieland, Markus Hecker, Scott Heximer, Thomas Korff
G protein-mediated signaling plays a decisive role in blood pressure regulation and the phenotype of vascular smooth muscle cells (VSMCs); however, the relevance of proteins that restrict G protein activity is not well characterized in this context. Here, we investigated the influence of regulator of G protein signaling 5 (RGS5), an inhibitor of Gαq/11 and Gαi/o activity, on blood pressure and the VSMC phenotype during experimental hypertension. In mice, loss of RGS5 did not affect baseline blood pressure, but prevented hypertension-induced structural remodeling...
April 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Lang He, Chaofen Zhao, Yunxiang Li, Guocheng Du, Kang Liu, Dandan Cui, Lina Tang, Xun Wu, Shimin Wen, Hong Chen
Antiangiogenic therapy, as a new anticancer method, can improve the anticancer effect of traditional therapies. Different antiangiogenic drugs may have different vascular normalization time windows. Whether the antiangiogenic treatment is within the vascular normalization time window is very important in the treatment of cancers. Previous studies have indicated that recombinant human endostatin (rh‑ES) can transiently normalize tumor microvessels. Yet the molecular mechanism and the time window of rh‑ES remains unclear...
January 2018: Molecular Medicine Reports
Kwangseok Hong, Min Li, Zahra Nourian, Gerald A Meininger, Michael A Hill
Studies suggest that arteriolar pressure-induced vasoconstriction can be initiated by GPCRs (G protein-coupled receptors), including the AT1 R (angiotensin II type 1 receptor). This raises the question, are such mechanisms regulated by negative feedback? The present studies examined whether RGS (regulators of G protein signaling) proteins in vascular smooth muscle cells are colocalized with the AT1 R when activated by mechanical stress or angiotensin II and whether this modulates AT1 R-mediated vasoconstriction...
December 2017: Hypertension
Laura A Madigan, Gordon S Wong, Elizabeth M Gordon, Wei-Sheng Chen, Nariman Balenga, Cynthia J Koziol-White, Reynold A Panettieri, Stewart J Levine, Kirk M Druey
A cardinal feature of asthma is airway hyperresponsiveness (AHR) to spasmogens, many of which activate G protein-coupled receptors (GPCRs) on airway smooth muscle (ASM) cells. Asthma subtypes associated with allergy are characterized by eosinophilic inflammation in the lung due to the type 2 immune response to allergens and proinflammatory mediators that promote AHR. The degree to which intrinsic abnormalities of ASM contribute to this phenotype remains unknown. The regulators of G protein signaling (RGS) proteins are a large group of intracellular proteins that inhibit GPCR signaling pathways...
January 2018: American Journal of Respiratory Cell and Molecular Biology
D J Luessen, H Sun, M M McGinnis, B A McCool, R Chen
Chronic alcohol exposure induces pronounced changes in GPCR-mediated G-protein signaling. Recent microarray and RNA-seq analyses suggest associations between alcohol abuse and the expression of genes involved in G-protein signaling. The activity of G-proteins (e.g. Gαi/o and Gαq) is negatively modulated by regulator of G-protein signaling (RGS) proteins which are implicated in drugs of abuse including alcohol. The present study used 7days of chronic intermittent ethanol exposure followed by 24h withdrawal (CIE) to investigate changes in mRNA and protein levels of G-protein subunit isoforms and RGS protein subtypes in rat prefrontal cortex, a region associated with cognitive deficit attributed to excessive alcohol drinking...
October 1, 2017: Brain Research
Chuanliang Liu, Qiongqiong Hu, Jia Jing, Yun Zhang, Jing Jin, Liulei Zhang, Lili Mu, Yumei Liu, Bo Sun, Tongshuai Zhang, Qingfei Kong, Guangyou Wang, Dandan Wang, Yao Zhang, Xijun Liu, Wei Zhao, Jinghua Wang, Tao Feng, Hulun Li
Regulator of G protein signaling 5 (RGS5) acts as a GTPase-activating protein (GAP) for the Gαi subunit and negatively regulates G protein-coupled receptor signaling. However, its presence and function in postmitotic differentiated primary neurons remains largely uncharacterized. During neural development, sonic hedgehog (Shh) signaling is involved in cell signaling pathways via Gαi activity. In particular, Shh signaling is essential for embryonic neural tube patterning, which has been implicated in neuronal polarization involving neurite outgrowth...
July 3, 2017: Molecular and Cellular Neurosciences
Justyna Różycka, Edyta Brzóska, Tomasz Skirecki
Pericytes, which are multi-potential stem cells, co-create the walls of the microvessels: capillaries, terminal arterioles and postcapillary venules. These cells are localized under the basement membrane, tightly encircling the endothelium. The most frequently mentioned molecular markers of pericytes include NG2 (neural-glial antigen 2), β-type platelet-derived growth factor receptor (PDGFRβ), smooth muscle α-actin (α-SMA), regulator of G protein signalling 5 (RGS5), the adhesion protein CD146 and nestin...
March 13, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
Yanyan Han, Yeting Wu, Chou Yang, Jing Huang, Yabing Guo, Li Liu, Ping Chen, Dongyun Wu, Junyun Liu, Jin Li, Xiangjun Zhou, Jinlin Hou
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common cancers in China and frequently occurs with chronic hepatitis B virus infection. To investigate whether cell-based cancer immunotherapy induces tumor specific immune responses in patients with HCC and provides clinical benefits, as well as to elucidate the most immunogenic tumor associated antigens (TAAs), multiple antigen stimulating cellular therapy (MASCT) was applied in addition to standard of care. METHODS: Mature dendritic cells (DCs) and activated T cells prepared for MASCT were generated from autologous peripheral blood mononuclear cells (PBMCs)...
March 22, 2017: Journal of Translational Medicine
R J Kern, C M Zarek, A K Lindholm-Perry, L A Kuehn, W M Snelling, H C Freetly, H C Cunningham, A M Meyer
Ruminal genes differentially expressed in crossbred beef steers from USMARC with variation in gain and feed intake were identified in a previous study. Several of the genes identified with expression patterns differing between animals with high gain-low feed intake and low gain-high feed intake were evaluated in a separate, unrelated population of Angus × Hereford beef steers from the University of Wyoming that was classified to differ in residual feed intake (RFI). Of the 17 genes tested, two were differentially expressed by RFI class in the Angus × Hereford animals...
February 2017: Animal Genetics
Aaron W James, Paul Hindle, Iain R Murray, Christopher C West, Tulyapruek Tawonsawatruk, Jia Shen, Greg Asatrian, Xinli Zhang, Vi Nguyen, A Hamish Simpson, Kang Ting, Bruno Péault, Chia Soo
Pericytes and other perivascular stem cells are of growing interest in orthopedics and tissue engineering. Long regarded as simple regulators of angiogenesis and blood pressure, pericytes are now recognized to have MSC (mesenchymal stem cell) characteristics, including multipotentiality, self-renewal, immunoregulatory functions, and diverse roles in tissue repair. Pericytes are typified by characteristic cell surface marker expression (including αSMA, CD146, PDGFRβ, NG2, RGS5, among others). Although alone no marker is absolutely specific for pericytes, collectively these markers appear to selectively identify an MSC-like pericyte...
March 2017: Pharmacology & Therapeutics
Anne J Grotenhuis, Aleksandra M Dudek, Gerald W Verhaegh, Katja K Aben, J Alfred Witjes, Lambertus A Kiemeney, Sita H Vermeulen
BACKGROUND: Many studies investigated the prognostic or predictive relevance of single nucleotide polymorphisms (SNPs) in biologically plausible genes in urinary bladder cancer (UBC) patients. Most published SNP associations have never been replicated in independent patient series. OBJECTIVE: To independently replicate all previously reported associations between germline SNPs and disease prognosis or treatment response in UBC. METHODS: A Pubmed search was performed to identify studies published by July 1, 2014 reporting on germline SNP associations with UBC prognosis or treatment response...
2016: Bladder Cancer
Benjamin L King, Viravuth P Yin
BACKGROUND: Although regenerative capacity is evident throughout the animal kingdom, it is not equally distributed throughout evolution. For instance, complex limb/appendage regeneration is muted in mammals but enhanced in amphibians and teleosts. The defining characteristic of limb/appendage regenerative systems is the formation of a dedifferentiated tissue, termed blastema, which serves as the progenitor reservoir for regenerating tissues. In order to identify a genetic signature that accompanies blastema formation, we employ next-generation sequencing to identify shared, differentially regulated mRNAs and noncoding RNAs in three different, highly regenerative animal systems: zebrafish caudal fins, bichir pectoral fins and axolotl forelimbs...
2016: PloS One
Miguel Romero, Rosario Jiménez, Marta Toral, Elvira León-Gómez, Manuel Gómez-Gúzman, Manuel Sánchez, María José Zarzuelo, Isabel Rodríguez-Gómez, Geraldine Rath, Juan Tamargo, Francisco Pérez-Vizcaíno, Chantal Dessy, Juan Duarte
Activation of peroxisome proliferator-activated receptor-β/δ (PPARβ) lowers blood pressure in genetic and mineralocorticoid-induced hypertension. Regulator of G-protein-coupled receptor signaling 5 (RGS5) protein, which interferes in angiotensin II (AngII) signaling, is a target gene to PPARβ The aim of the present study was to examine whether PPARβ activation in resistance arteries and brain tissues prevents the elevated blood pressure in AngII-induced hypertension and evaluate the role of RGS5 in this effect...
July 2016: Journal of Pharmacology and Experimental Therapeutics
Caroline E Gargett, Shanti Gurung
No abstract text is available yet for this article.
June 2016: Biology of Reproduction
Jia Shen, Swati Shrestha, P Nagesh Rao, Greg Asatrian, Michelle A Scott, Vi Nguyen, Paulina Giacomelli, Chia Soo, Kang Ting, Fritz C Eilber, Bruno Peault, Sarah M Dry, Aaron W James
Pericytes are modified smooth muscle cells that closely enwrap small blood vessels, regulating and supporting the microvasculature through direct endothelial contact. Pericytes demonstrate a distinct immunohistochemical profile, including expression of smooth muscle actin, CD146, platelet-derived growth factor receptor β, and regulator of G-protein signaling 5. Previously, pericyte-related antigens have been observed to be present among a group of soft tissue tumors with a perivascular growth pattern, including glomus tumor, myopericytoma, and angioleiomyoma...
August 2016: Human Pathology
Zhiqiang Wang, He Huang, Wangwei He, Bin Kong, He Hu, Yang Fan, Jiafen Liao, Lei Wang, Yang Mei, Wanli Liu, Xiaoju Xiong, Jianye Peng, Yali Xiao, Dan Huang, Dajun Quan, Qi Li, Liang Xiong, Peng Zhong, Guangji Wang
Ischemic heart disease is one of the most common diseases in modern society. Ischemic myocardium can be salvaged by vascular recanalization therapy, but its benefit is attenuated by injury that can occur during reperfusion. And apoptotic cell death plays an important part in myocardial ischemia-reperfusion (IR) injury. Regulator of G-protein signaling 5 (RGS5), highly expressed in different cell types of the human adult heart, is a guanosine triphosphatase-activating protein to inhibit many signaling pathways such as c-Jun NH2-terminal kinase 1/2 (JNK1/2) and p38 which promote cardiac IR-induced apoptosis...
April 29, 2016: Biochemical and Biophysical Research Communications
Nicolás González-Vacarezza, Isabel Alonso, Gustavo Arroyo, Jorge Martínez, Fernando De Andrés, Adrián LLerena, Francisco Estévez-Carrizo
Bevacizumab was the first molecular-targeted antiangiogenic therapy approved for the treatment of metastatic colorectal cancer. Until now, there are no predictive biomarkers available to decide the prescription of bevacizumab in patients with colorectal cancer. The purposes of this review were to provide a critical appraisal of the evidence and to identify possible predictive genetic biomarkers. A literature search was performed to identify studies that determine different levels of treatment response between patients stratified according to defined biomarkers...
June 1, 2016: Drug Metabolism and Personalized Therapy
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