keyword
MENU ▼
Read by QxMD icon Read
search

RNA Imaging

keyword
https://www.readbyqxmd.com/read/28633080/disruptive-non-disruptive-applications-of-crispr-cas9
#1
REVIEW
Jonathan L Schmid-Burgk
The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerful tool for disrupting protein-coding genes. Cas9 cleaves target sites to generate a double-strand break (DSB) that is repaired via an error-prone repair process, leading to insertion/deletion mutations and gene knockouts...
June 17, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28623900/characterization-of-microrna-200-pathway-in-ovarian-cancer-and-serous-intraepithelial-carcinoma-of-fallopian-tube
#2
Junzheng Yang, Yilan Zhou, Shu-Kay Ng, Kuan-Chun Huang, Xiaoyan Ni, Pui-Wah Choi, Kathleen Hasselblatt, Michael G Muto, William R Welch, Ross S Berkowitz, Shu-Wing Ng
BACKGROUND: Ovarian cancer is the leading cause of death among gynecologic diseases in Western countries. We have previously identified a miR-200-E-cadherin axis that plays an important role in ovarian inclusion cyst formation and tumor invasion. The purpose of this study was to determine if the miR-200 pathway is involved in the early stages of ovarian cancer pathogenesis by studying the expression levels of the pathway components in a panel of clinical ovarian tissues, and fallopian tube tissues harboring serous tubal intraepithelial carcinomas (STICs), a suggested precursor lesion for high-grade serous tumors...
June 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28620356/quantifying-biochemical-alterations-in-brown-and-subcutaneous-white-adipose-tissues-of-mice-using-fourier-transform-infrared-widefield-imaging
#3
Ebrahim Aboualizadeh, Owen T Carmichael, Ping He, Diana C Albarado, Christopher D Morrison, Carol J Hirschmugl
Stimulating increased thermogenic activity in adipose tissue is an important biological target for obesity treatment, and label-free imaging techniques with the potential to quantify stimulation-associated biochemical changes to the adipose tissue are highly sought after. In this study, we used spatially resolved Fourier transform infrared (FTIR) imaging to quantify biochemical changes caused by cold exposure in the brown and subcutaneous white adipose tissues (BAT and s-WAT) of 6 week-old C57BL6 mice exposed to 30°C (N = 5), 24°C (N = 5), and 10°C (N = 5) conditions for 10 days...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28609214/the-potyvirus-particle-recruits-the-plant-translation-initiation-factor-eif4e-by-means-of-the-vpg-covalently-linked-to-the-viral-rna
#4
Geneviève Tavert-Roudet, Agnès Anne, Amandine Barra, Arnaud Chovin, Christophe Demaille, Thierry Michon
The VPg of potyviruses is a protein covalently linked to the 5' end of viral RNA. It interacts with eIF4E, a component of the cellular translation initiation complex. It has been suggested that the 5' RNA linked VPg could mimic the cellular mRNA cap, promoting synthesis of viral proteins. We report here evidences for evidence for recruitment of the plant eIF4E by Lettuce mosaic virus (LMV, potyvirus) particles via the 5' RNA linked VPg. Analysis of the viral population was performed by ELISA-based tests, either with crude extracts of LMV-infected tissues or purified viral particles...
June 13, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28605379/voltage-clamp-fluorometry-in-xenopus-oocytes-using-fluorescent-unnatural-amino-acids
#5
Tanja Kalstrup, Rikard Blunck
Voltage-Clamp Fluorometry (VCF) has been the technique of choice to investigate the structure and function of electrogenic membrane proteins where real-time measurements of fluorescence and currents simultaneously report on local rearrangements and global function, respectively(1). While high-resolution structural techniques such as cryo-electron microscopy or X-ray crystallography provide static images of the proteins of interest, VCF provides dynamic structural data that allows us to link the structural rearrangements (fluorescence) to dynamic functional data (electrophysiology)...
May 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28604724/visualizing-adenosine-to-inosine-rna-editing-in-single-mammalian-cells
#6
Ian A Mellis, Rohit Gupte, Arjun Raj, Sara H Rouhanifard
Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about the biology of this conversion remain unknown because of a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine-edited transcripts in situ. We found that editing of the GRIA2, EIF2AK2, and NUP43 transcripts is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells, while GRIA2 editing levels vary...
June 12, 2017: Nature Methods
https://www.readbyqxmd.com/read/28595542/synthesis-of-99mtc-rifabutin-a-potential-tuberculosis-radiodiagnostic-agent
#7
Syed Qaiser Shah, Mamoona Alam
BACKGROUND: Rifabutin (RFN) is bactericidal antibiotic with a very broad spectrum of activity against gram positive & gram negative organisms including Pseudomonas aeruginosa and specifically Mycobacterium tuberculosis. OBJECTIVE: RFN inhibits DNA dependent RNA polymerase activity in susceptible cells. In the instant work, the therapeutic characteristics of RFN were intended for diagnostic rationale by labeling it with 99mTc (Technetium-99m). The 99mTc labeled RFN (99mTc-RFN) was investigated for labeling capacity, steadiness in saline & serum, in vitro Mycobacterium tuberculosis (MBT) uptake & distribution in MBT stained animal model rats...
June 5, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28592854/transcriptomic-analysis-of-short-fruit-1-sf1-reveals-new-insights-into-the-variation-of-fruit-related-traits-in-cucumis-sativus
#8
Lina Wang, Chenxing Cao, Shuangshuang Zheng, Haiyang Zhang, Panjing Liu, Qian Ge, Jinrui Li, Zhonghai Ren
Fruit size is an important quality trait in different market classes of Cucumis sativus L., an economically important vegetable cultivated worldwide, but the genetic and molecular mechanisms that control fruit size are largely unknown. In this study, we isolated a natural cucumber mutant, short fruit 1 (sf1), caused by a single recessive Mendelian factor, from the North China-type inbred line CNS2. In addition to significantly decreased fruit length, other fruit-related phenotypic variations were also observed in sf1 compared to the wild-type (WT) phenotype, indicating that sf1 might have pleiotropic effects...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28591846/visualization-of-conformational-variability-in-the-domains-of-long-single-stranded-rna-molecules
#9
Jamie L Gilmore, Aiko Yoshida, James A Hejna, Kunio Takeyasu
We demonstrate an application of atomic force microscopy (AFM) for the structural analysis of long single-stranded RNA (>1 kb), focusing on 28S ribosomal RNA (rRNA). Generally, optimization of the conditions required to obtain three-dimensional (3D) structures of long RNA molecules is a challenging or nearly impossible process. In this study, we overcome these limitations by developing a method using AFM imaging combined with automated, MATLAB-based image analysis algorithms for extracting information about the domain organization of single RNA molecules...
June 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28590298/diagnostic-and-prognostic-implications-of-exercise-treadmill-and-rest-first-pass-radionuclide-angiography-in-patients-with-pulmonary-hypertension
#10
Yen-Wen Wu, Pei-Ying Hsu, Yen-Hung Lin, Mei-Fang Cheng, Chi-Lun Ko, Yih-Hwen Huang, Ruoh-Fang Yen, Jin-Shing Chen, Jou-Wei Lin, Hsao-Hsun Hsu
PURPOSE: Pulmonary hypertension (PH) is characterized by abnormally increased pulmonary vascular pressure, leading to deteriorated right ventricular function and premature death. Pulmonary mean transit time (PMTT) and biventricular function response to exercise in first-pass radionuclide angiography (FP-RNA) may provide early detection and timely disease monitoring of PH. This study aimed to investigate the diagnostic and prognostic values of this imaging modality in PH patients. METHODS: Left and right ventricular ejection fraction (LVEF/RVEF) and PMTT at rest and immediately after exercise treadmill test were measured by FP-RNA in 77 consecutive patients with clinical presentations suggestive of PH (aged 46 ± 15 years, 33 men), mostly with symptoms of unexplained progressive dyspnea...
June 5, 2017: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/28588149/tumor-metabolic-features-identified-by-fdg-pet-correlates-with-gene-networks-of-immune-cell-microenvironment-in-head-and-neck-cancer
#11
Kwon Joong Na, Hongyoon Choi
The importance of FDG PET in imaging head and neck squamous cell carcinoma (HNSCC) has grown in recent decades. Since PET has prognostic values, and provides functional and molecular information in HNSCC, the genetic and biologic backgrounds associated with PET parameters are of great interests. Here, as a systems biology approach, we aimed to investigate gene networks associated with tumor metabolism and their biological function using RNA sequence and FDG PET data. Methods: Using RNA sequence data of HNSCC downloaded from TCGA data portal, we constructed gene coexpression network...
June 6, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28587718/%C3%AE-amyloid-precursor-protein-intracellular-domain-controls-mitochondrial-function-by-modulating-phosphatase-and-tensin-homolog-induced-kinase-1-transcription-in-cells-and-in-alzheimer-mice-models
#12
Thomas Goiran, Eric Duplan, Mounia Chami, Alexandre Bourgeois, Wejdane El Manaa, Lila Rouland, Julie Dunys, Inger Lauritzen, Han You, Vuk Stambolic, Maria-Grazia Biféri, Martine Barkats, Sanjay W Pimplikar, Nicolas Sergeant, Morvane Colin, Vanessa A Morais, Raphaelle Pardossi-Piquard, Frédéric Checler, Cristine Alves da Costa
BACKGROUND: Mitophagy and mitochondrial dynamics alterations are two major hallmarks of neurodegenerative diseases. Dysfunctional mitochondria accumulate in Alzheimer's disease-affected brains by yet unexplained mechanisms. METHODS: We combined cell biology, molecular biology, and pharmacological approaches to unravel a novel molecular pathway by which presenilins control phosphatase and tensin homolog-induced kinase 1 (Pink-1) expression and transcription. In vivo approaches were carried out on various transgenic and knockout animals as well as in adeno-associated virus-infected mice...
May 3, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/28585897/interrelationships-of-dictyocaulus-spp-in-wild-ruminants-with-morphological-description-of-dictyocaulus-cervi-n-sp-nematoda-trichostrongyloidea-from-red-deer-cervus-elaphus
#13
Anna Maria Pyziel, Zdzisław Laskowski, Aleksander W Demiaszkiewicz, Johan Höglund
Lungworms from the genus Dictyocaulus cause parasitic bronchitis (dictyocaulosis) characterized by coughing and severe lung pathology in both domestic and wild ruminants. In this study we investigated the interrelationships of Dictyocaulus spp. from European bison (Bison bonasus L.), roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by nucleotide sequence analysis spanning the 18S RNA gene (SSU) and internal transcribed spacer 2 (ITS2) regions of the ribosomal gene array as well as the mitochondrial cytochrome c oxidase subunit 1 (cox1)...
June 6, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28585762/upregulation-of-the-long-non-coding-rna-falec-promotes-proliferation-and-migration-of-prostate-cancer-cell-lines-and-predicts-prognosis-of-pca-patients
#14
Ruizhe Zhao, Feng Sun, Xiaoyu Bei, Xingjie Wang, Yiping Zhu, Chenyi Jiang, Fujun Zhao, Bangmin Han, Shujie Xia
BACKGROUND: LncRNAs are aberrantly expressed in various cancer types and were found to be a responsible prognosis biomarker and therapeutic target of many human cancers. METHODS: In this study, we characterized the expression profile of FALEC in prostate cancer and paired histologically normal tissues. Additionally, biological function of FALEC in prostate cancer cell lines was determined by in vitro and in vivo assays. RESULTS: In a total of 85 patients, FALEC expression was significantly increased in clinical PCa tissues compared to adjacent normal tissues, and can be considered as an independent prognostic factor in patients with PCa...
June 6, 2017: Prostate
https://www.readbyqxmd.com/read/28580104/highly-specific-imaging-of-mrna-in-single-cells-by-target-rna-initiated-rolling-circle-amplification
#15
Ruijie Deng, Kaixiang Zhang, Yupeng Sun, Xiaojun Ren, Jinghong Li
Detection of single-cell gene expression with high spatial and sequence resolution is a key challenge in single cell biology. Herein, we propose a robust method for the direct detection of mRNA, termed target RNA-initiated rolling circle amplification, which enables imaging of mRNA with single-nucleotide and near-single-molecule resolution in single cells. By utilizing a Splint R ligase capable of efficiently catalyzing the ligation of a padlock probe by the target RNA, the method can enable the efficient detection of mRNA without reverse transcription (detection efficiency over 20%)...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28579403/cefra-seq-systematic-mapping-of-rna-subcellular-distribution-properties-through-cell-fractionation-coupled-to-deep-sequencing
#16
Fabio Alexis Lefebvre, Neal Cody, Louis Philip Benoit Bouvrette, Julie Bergalet, Xiaofeng Wang, Eric Lécuyer
The subcellular trafficking of RNA molecules is a conserved feature of eukaryotic cells and plays key functions in diverse processes implicating polarised cellular activities. Large-scale imaging and subcellular transcriptomic studies suggest that regulated RNA localization is a highly prevalent process that appears to be disrupted in several neuromuscular disorders. These features underline the importance and usefulness of implementing procedures to assess global transcriptome subcellular distribution properties...
June 1, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28578487/gambogic-acid-induces-cell-apoptosis-and-inhibits-mapk-pathway-in-pten-p53-prostate-cancer-cells-in-vitro-and-ex-vivo
#17
Hong Pan, Li-Yuan Lu, Xue-Qian Wang, Bin-Xue Li, Kathleen Kelly, Hong-Sheng Lin
OBJECTIVE: To investigate the effect of gambogic acid (GA) on the growth and cell death of castrate resistant prostate cancer (PC) with phosphate and tension homology (PTEN) and p53 genes deleted in vitro and ex vivo, and elucidate the underlying possible molecular mechanisms. METHODS: PTEN(-/-)/p53(-/-) PC cells and Los Angeles prostate cancer-4 (LAPC-4) cells were treated with GA for 24 h and 48 h, then cell viability was determined by cell proliferation assay...
June 3, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28575868/increased-breast-tissue-receptor-activator-of-nuclear-factor-%C3%AE%C2%BAb-ligand-rankl-gene-expression-is-associated-with-higher-mammographic-density-in-premenopausal-women
#18
Adetunji T Toriola, Ha X Dang, Ian S Hagemann, Catherine M Appleton, Graham A Colditz, Jingqin Luo, Christopher A Maher
Increased mammographic breast density is associated with a 4-6-fold increased risk of breast cancer, yet lifestyle factors that can reduce dense breasts are yet to be identified, and viable prevention strategies to reduce breast density-associated breast cancer development are yet to be developed. We investigated the associations of breast tissue receptor activator of nuclear factor-κB (RANK) pathway gene expression with mammographic density in 48 premenopausal women, with no previous history of cancer. Gene expression levels were measured in total RNA isolated from formalin-fixed paraffin-embedded breast tissue samples, using the NanoString nCounter platform...
May 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28574633/a-general-mechanism-of-photoconversion-of-green-to-red-fluorescent-proteins-based-on-blue-and-infrared-light-reduces-phototoxicity-in-live-cell-single-molecule-imaging
#19
Bartosz Turkowyd, Alexander Balinovic, David Virant, Haruko Gladys Gölz Carnero, Fabienne Caldana, Ulrike Endesfelder
Photoconversion of fluorescent proteins by blue and complementary near-infrared light, termed primed conversion (PC), is a mechanism recently discovered for Dendra2. We demonstrate that controlling the conformation of arginine at residue 66 by threonine at residue 69 of fluorescent proteins from Anthozoan families (Dendra2, mMaple, Eos, mKikGR, pcDronpa protein families) represents a general route to facilitate PC. Mutations of alanine 159 or serine 173, which are known to influence chromophore flexibility and allow for reversible photoswitching, prevent PC...
June 2, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28574255/gold-nanoparticle-size-and-shape-effects-on-cellular-uptake-and-intracellular-distribution-of-sirna-nanoconstructs
#20
Jun Yue, Timothy Joel Feliciano, Wenlong Li, Andrew Lee, Teri W Odom
Gold nanoparticles (AuNPs) show potential for transfecting target cells with small interfering RNA (siRNA), but the influence of key design parameters such as the size and shape of the particle core is incomplete. This paper describes a side-by-side comparison of the in vitro response of U87 glioblastoma cells to different formulations of siRNA-conjugated gold nanoconstructs targeting the expression of isocitrate dehydrogenase 1 (IDH1) based on 13 nm spheres, 50 nm spheres, and 40 nm stars. 50 nm spheres and 40 nm stars showed much higher uptake efficiency compared to 13 nm spheres...
June 21, 2017: Bioconjugate Chemistry
keyword
keyword
40843
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"