keyword
https://read.qxmd.com/read/38421433/analyzing-microbial-community-and-volatile-compound-profiles-in-the-fermentation-of-cigar-tobacco-leaves
#21
JOURNAL ARTICLE
Mingzhu Zhang, Dongfeng Guo, Haiqing Wang, Guanglong Wu, Yaqi Shi, Jinlong Zhou, Eryong Zhao, Tianfei Zheng, Xingjiang Li
Variations in industrial fermentation techniques have a significant impact on the fermentation of cigar tobacco leaves (CTLs), consequently influencing the aromatic attributes of the resulting cigars. The entire fermentation process of CTLs can be categorized into three distinct phases: phase 1 (CTLs prior to moisture regain), phase 2 (CTLs post-moisture regain and pile fermentation), and phase 3 (CTLs after fermentation and drying). These phases were determined based on the dynamic changes in microbial community diversity...
February 29, 2024: Applied Microbiology and Biotechnology
https://read.qxmd.com/read/38414081/the-mechanistic-role-of-natural-antimicrobials-in-preventing-staphylococcus-aureus-invasion-of-mac-t-cells-using-an-in-vitro-mastitis-model
#22
JOURNAL ARTICLE
Igori Balta, David McCleery, Saida Roxana Feier David, Elena Pet, Ducu Stef, Tiberiu Iancu, Ioan Pet, Lavinia Stef, Nicolae Corcionivoschi
BACKGROUND: Starting primarily as an inflammation of the mammary gland, mastitis is frequently driven by infectious agents such as Staphylococcus aureus. Mastitis has a large economic impact globally, which includes diagnostic, treatment, and the production costs not to mention the potential milk contamination with antimicrobial residues. Currently, mastitis prevention and cure depends on intramammary infusion of antimicrobials, yet, their overuse risks engendering resistant pathogens, posing further threats to livestock...
February 27, 2024: Irish Veterinary Journal
https://read.qxmd.com/read/38408673/decreased-transport-of-nano-and-micro-plastics-in-the-presence-of-low-molecular-weight-organic-acids-in-saturated-quartz-sand
#23
JOURNAL ARTICLE
Yanan Liu, Genyao Gu, Jizhe Lu, Luxiang Zhu, Quanyuan Chen, Hyunjung Kim, Jiajun Wang, Peng Ji, Li Cai
Low-molecular-weight organic acids (LMWOAs) and nano- and micro-plastics (NPs and MPs) are both widely distributed in terrestrial systems. To better understand the influence of LMWOAs on the transport of NPs and MPs, the effects of 0.5 mM citric- (CA), malic- (MA), and tartaric- (TA) acid on the transport of nano- (0.51 μm, PS NPs) and micro- (1.1 μm, PS MPs) polystyrene particles (2 mg L-1 ) in saturated quartz sand were investigated. All three LMWOAs decreased the transport of PS NPs and MPs, regardless of ionic composition or strength (0...
February 24, 2024: Science of the Total Environment
https://read.qxmd.com/read/38407053/enhancing-l-malic-acid-production-in-aspergillus-niger-via-natural-activation-of-stha-gene-expression
#24
JOURNAL ARTICLE
Dongdong Yang, Yingyan Xu, Li Mo, Man Shi, Na Wu, Ling Lu, Feng Xue, Qing Xu, Chi Zhang
The efficient production of l-malic acid using Aspergillus niger requires overcoming challenges in synthesis efficiency and excessive byproduct buildup. This study addresses these hurdles, improving the activity of NADH-dependent malate dehydrogenase (Mdh) in the early stages of the fermentation process. By employing a constitutive promoter to express the Escherichia coli sthA responsible for the transfer of reducing equivalents between NAD(H) and NADP(H) in A. niger , the l-malic acid production was significantly elevated...
February 26, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38404595/antagonistic-properties-of-lactiplantibacillus-plantarum-mysvb1-against-alternaria-alternata-a-putative-probiotic-strain-isolated-from-the-banyan-tree-fruit
#25
JOURNAL ARTICLE
R Vasundaradevi, M Sarvajith, Rakesh Somashekaraiah, Adithi Gunduraj, M Y Sreenivasa
Alternaria alternata , a notorious phytopathogenic fungus, has been documented to infect several plant species, leading to the loss of agricultural commodities and resulting in significant economic losses. Lactic acid bacteria (LAB) hold immense promise as biocontrol candidates. However, the potential of LABs derived from fruits remains largely unexplored. In this study, several LABs were isolated from tropical fruit and assessed for their probiotic and antifungal properties. A total of fifty-five LABs were successfully isolated from seven distinct fruits...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38403343/-screening-of-bioactive-components-endowing-hawthorn-with-turbidity-eliminating-and-lipid-lowering-functions-and-development-of-quality-control-method-of-hawthorn
#26
JOURNAL ARTICLE
Fei Sun, Xiang-Qin Wu, Qiong He, Yu-Hua Cao, Jian-Gang Wang, Sheng-Wang Liang, Shu-Mei Wang
Hawthorn has the efficacy of eliminating turbidity and lowering the blood lipid level, and it is used for treating hyperlipidemia in clinic. However, the bioactive components of hawthorn are still unclear. In this study, the spectrum-effect relationship was employed to screen the bioactive components of hawthorn in the treatment of hyperlipidemia, and then the bioactive components screened out were verified in vivo. Furthermore, the quality control method for hawthorn was developed based on liquid chromatography-mass spectrometry(LC-MS)...
January 2024: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://read.qxmd.com/read/38401045/transcriptomics-and-metabolomics-unveil-the-neuroprotection-mechanism-of-angong-niuhuang-agnh-pill-against-ischaemic-stroke-injury
#27
JOURNAL ARTICLE
Liangliang Tian, Guangzhao Cao, Xiaotong Zhu, Lihan Wang, Jingyi Hou, Yi Zhang, He Xu, Lixia Wang, Shicong Wang, Chen Zhao, Hongjun Yang, Jingjing Zhang
As a famous prescription in China, AnGong NiuHuang (AGNH) pill exerts good neuroprotection for ischaemic stroke (IS), but its mechanism is still unclear. In this study, the neuroprotection of AGNH was evaluated in the rat IS model which were established with the surgery of middle cerebral artery occlusion (MCAO), and the potential mechanism was elucidated by transcriptomic analysis and metabolomic analysis. AGNH treatment obviously decreased the infarct volume and Zea-Longa 5-point neurological deficit scores, improved the survival percentage of rats, regional cerebral blood flow (rCBF), and rat activity distance and activity time...
February 24, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38397851/maternal-malic-acid-may-ameliorate-oxidative-stress-and-inflammation-in-sows-through-modulating-gut-microbiota-and-host-metabolic-profiles-during-late-pregnancy
#28
JOURNAL ARTICLE
Meixia Chen, Ying Zhao, Shuang Li, Zhuo Chang, Hui Liu, Dongyan Zhang, Sixin Wang, Xin Zhang, Jing Wang
Sows suffer oxidative stress and inflammation induced by metabolic burden during late pregnancy, which negatively regulates reproductive and lactating performances. We previously found that L-malic acid (MA) alleviated oxidative stress and inflammation and improved reproductive performances in sows. However, the mechanism underlying the MA's positive effects remains unexplored. Here, twenty Large White × Landrace sows with similar parity were randomly divided into two groups and fed with a basal diet or a diet supplemented with 2% L-malic acid complex from day 85 of gestation to delivery...
February 19, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38397827/the-health-promoting-quality-attributes-polyphenols-iridoids-and-antioxidant-activity-during-the-development-and-ripening-of-cornelian-cherry-cornus-mas-l
#29
JOURNAL ARTICLE
Dominika Przybylska, Alicja Z Kucharska, Narcyz Piórecki, Tomasz Sozański
This study defined the physicochemical attributes, composition, and antioxidant capacity of four Polish cultivars of cornelian cherry (CC) at six stages of development and ripening. A total of 52 metabolites were identified by UPLC-ESI-qTOF-MS/MS and quantified by HPLC-PDA. In general, phenolic acids, hydrolyzable tannins, flavonols, iridoids, antioxidant activity, organic acids, and vitamin C decreased, while anthocyanins, malic acid, sugars, and titratable acidity increased. For the first time, we determined the evolution of the CC chemical properties and the metabolic behavior and quantified the individual compounds, and groups of compounds during ripening, in particular gallotannins, ellagitannins, iridoids, and organic acids...
February 13, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38397176/comparison-of-growth-performance-carcass-properties-fatty-acid-profile-and-genes-involved-in-fat-metabolism-in-nanyang-and-landrace-pigs
#30
JOURNAL ARTICLE
Jinzhou Zhang, Shuaitao Meng, Heming Wang, Chuankuan Zhang, Zhe Sun, Luyao Huang, Zhiguo Miao
This study compared the growth, carcass properties, fatty acid profile, lipid-producing enzyme activity, and expression pattern of genes involved in fat metabolism in Nanyang and Landrace pigs. In the study, 32 Nanyang (22.16 ± 0.59 kg) and 32 Landrace barrows (21.37 ± 0.57 kg) were selected and divided into two groups, each with eight pens and four pigs per pen. The trial period lasted 90 days. The findings showed that the Nanyang pigs had lower average daily weight gain and lean percentage and higher average backfat thickness and lipogenic enzyme activities, including for acetyl-CoA carboxylase, glucose-6-phosphate dehydrogenase, malic enzyme, and fatty acid synthase, than the Landrace pigs...
January 30, 2024: Genes
https://read.qxmd.com/read/38392341/differences-of-pine-wood-nematode-bursaphelenchus-xylophilus-developmental-stages-under-high-osmotic-pressure-stress
#31
JOURNAL ARTICLE
Shuting Wang, Qiaoli Chen, Feng Wang
Under ion imbalance, water deficiency, and salt stress, the osmotic pressure of the tree sap increases, and pine wood nematodes ( Bursaphelenchus xylophilus , PWN) parasitizing in the trees may be subjected to high-osmotic-pressure stress. KCl, L-malic acid, sucrose, and glycerol solutions were used as osmolytes to explore the highest osmotic concentration that PWN can tolerate. Survival analysis showed that when the treatment concentration exceeded 90%, only a few nematodes in the glycerol group survived under 6 h treatment, and most of the survivors were third-stage dispersal juveniles (DJ3)...
February 16, 2024: Biology
https://read.qxmd.com/read/38382318/mass-spectrometry-imaging-based-metabolomics-highlights-spatial-metabolic-alterations-in-three-types-of-liver-injuries
#32
JOURNAL ARTICLE
Zihan Zhu, Yun Zhang, Jun Li, Yuhao Han, Lei Wang, Yaqi Zhang, Haoyuan Geng, Yurong Zheng, Xiao Wang, Chenglong Sun, Baoguo Li, Panpan Chen
Liver's distinctive function renders it highly susceptible to diverse damage sources. Characterizing the metabolic profiles and spatial signatures in different liver injuries is imperative for early diagnosis and etiology-oriented treatment. In this comparative study, we conducted whole-body spatial metabolomics on zebrafish with liver injury induced by ethanol (EtOH), acetaminophen (APAP), and thioacetamide (TAA). The two specific levels, the whole-body and liver-specific metabolic profiles, as well as their regional distributions, were systematically mapped in situ by mass spectrometry imaging, which is distinct from conventional LC-MS and GC-MS methods...
February 10, 2024: Journal of Pharmaceutical and Biomedical Analysis
https://read.qxmd.com/read/38379805/a-comparative-study-of-lachancea-thermotolerans-fermentative-performance-under-standardized-wine-production-conditions
#33
JOURNAL ARTICLE
Javier Vicente, Luka Vladic, Eva Navascués, Silvia Brezina, Antonio Santos, Fernando Calderón, Wendu Tesfaye, Domingo Marquina, Doris Rauhut, Santiago Benito
The study explores diverse strains of Lachancea thermotolerans in single-inoculum wine fermentation conditions using synthetic grape must. It aims to analyze the role of the species without external influences like other microorganisms or natural grape must variability. Commercial strains and selected vineyard isolates, untested together previously, are assessed. The research evaluates volatile and non-volatile chemical compounds in final wine, revealing significant strain-based variations. L. thermotolerans notably produces lactic acid and consumes malic acid, exhibiting moderate ethanol levels...
March 30, 2024: Food chemistry: X
https://read.qxmd.com/read/38373883/prohexadione-calcium-improves-grape-quality-by-regulating-endogenous-hormones-sugar-and-acid-metabolism-and-related-enzyme-activities-in-grape-berries
#34
JOURNAL ARTICLE
Dou Li, Jiangshan Yang, Zibo Dai, Yajuan Chen, Zhang Shao, Chunheng Wang, Xin Jin, Yuhang Wang, Lidan Feng
Prohexadione-Calcium (Pro-Ca) plays key roles in improving fruit quality and yield by regulating various aspects of plant growth. However, the effects of how Pro-Ca regulates the regulation of sugar and acid balance and its impact on the production of volatile aroma substances during fruit growth and development are poorly understood. In this study, the Pro-Ca solutions developed at concentrations of 200, 400, 600 and 800 mg·L-1 were sprayed on the entire "Chardonnay" grape tree 22, 42, 62 and 82 days after initial flowering...
February 19, 2024: BMC Plant Biology
https://read.qxmd.com/read/38373510/surface-display-system-of-bacillus-subtilis-a-promising-approach-for-improving-the-stability-and-applications-of-cellobiose-dehydrogenase
#35
JOURNAL ARTICLE
Zhengfen Wu, Pengfei Li, Xihua Chen, Yong Feng, Yi Ma, Zhong Ni, Daochen Zhu, Huayou Chen
Cellobiose dehydrogenase (CDH) plays a crucial role in lignocellulose degradation and bioelectrochemical industries, making it highly in demand. However, the production and purification of CDH through fungal heterologous expression methods is time-consuming, costly, and challenging. In this study, we successfully displayed Pycnoporus sanguineus CDH (psCDH) on the surface of Bacillus subtilis spores for the first time. Enzymatic characterization revealed that spore surface display enhanced the tolerance of psCDH to high temperature (80 °C) and low pH levels (3...
February 17, 2024: Protein Expression and Purification
https://read.qxmd.com/read/38372212/valorization-of-cabbage-waste-as-a-feedstock-for-microbial-polyhydroxyalkanoate-production-optimizing-hydrolysis-conditions-and-polyhydroxyalkanoate-production
#36
JOURNAL ARTICLE
Jung Eun Yang, Hye Sung Jeon, Seulbi Kim, Yeong Yeol Kim, Jong-Cheol Kim, Ho Myeong Kim, In Min Hwang, Hae Woong Park
Establishing a platform for the bioconversion of waste resources into value-added compounds is critical for achieving a sustainable and eco-friendly economy. Herein, we produced polyhydroxyalkanoate via microbial fermentation using cabbage waste as a feedstock and metabolically engineered Escherichia coli . For this, the hydrolysis conditions of cabbage waste were optimized by focusing on parameters such as substrate and enzyme concentrations to enhance the saccharification efficiency. The phaABC operon, which encodes key enzymes responsible for polyhydroxyalkanoate biosynthesis in Ralstonia eutropha H16, was overexpressed in E...
February 19, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38370263/using-ethanol-as-postharvest-treatment-to-increase-polyphenols-and-anthocyanins-in-wine-grape
#37
JOURNAL ARTICLE
Modesti Margherita, Alfieri Gianmarco, Magri Anna, Forniti Roberto, Ferri Serena, Petriccione Milena, Taglieri Isabella, Mencarelli Fabio, Bellincontro Andrea
Red wine grapes are qualitatively evaluated for their content in polyphenols and anthocyanins. Due to certain conditions (weather, latitude, temperature), the concentration of these compounds may be not at the right level for reaching a high-quality wine, thus postharvest technologies can be operated as a remediation strategy. Ethanol is a secondary volatile metabolite and its application has been demonstrated to delay fruit ripening, to reduce decay, and to increase secondary metabolites. The present study investigates the effects of ethanol post-harvest application on wine grapes' metabolism and composition...
February 29, 2024: Heliyon
https://read.qxmd.com/read/38367786/effects-of-microwave-radiation-on-the-physicochemical-properties-structure-and-digestibility-of-the-synthesis-of-different-crystal-forms-of-malic-acid-starch-ester
#38
JOURNAL ARTICLE
Wei Wang, Aijun Hu, Shiwei Liu, Jie He, Jie Zheng
The effects of microwave combined with L-malic acid treatment on the degree of substitution (DS), structure, physicochemical properties, and digestibility of sweet potato starch (A-type), potato starch (B-type), and pea starch (C-type) were evaluated. The order of DS obtained was: DSM-POS  > DSM-SPS  > DSM-PES . Fourier transform-infrared spectroscopy (FT-IR) showed that the obtained modified starch produced a new absorption band at 1735 cm-1 . Scanning electron microscopy (SEM) and polarized light microscopy indicated that different types of native starches exhibited different granular morphologies and appeared to have different degrees of damage, but still had polarized crosses after modification...
February 15, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38365225/molecular-regulatory-mechanisms-of-staminate-strobilus-development-and-dehiscence-in-torreya-grandis
#39
JOURNAL ARTICLE
Weijie Chen, Baofeng Jiang, Hao Zeng, Zhihui Liu, Wenchao Chen, Shan Zheng, Jiasheng Wu, Heqiang Lou
Gymnosperms are mostly dioecious, and their staminate strobili undergo a longer developmental period than those of angiosperms. However, the underlying molecular mechanisms remain unclear. This study aimed to identify key genes and pathways involved in staminate strobilus development and dehiscence in Torreya grandis. Through weighted gene co-expression network analysis (WGCNA), we identified fast elongation-related genes enriched in carbon metabolism and auxin signal transduction, whereas dehiscence-related genes were abundant in alpha-linolenic acid metabolism and the phenylpropanoid pathway...
February 14, 2024: Plant Physiology
https://read.qxmd.com/read/38362104/optimization-of-tomato-solanum-lycopersicum-l-juice-fermentation-process-and-analysis-of-its-metabolites-during-fermentation
#40
JOURNAL ARTICLE
Lei Zhao, Ruxianguli Maimaitiyiming, Jingyang Hong, Liang Wang, Ying Mu, Bingze Liu, Huimin Zhang, Keping Chen, Aihemaitijiang Aihaiti
Tomato ( Solanum lycopersicum L.) is a nutritious fruit and vegetable. Fermentation can be used to enhance their nutritional value. In this study, the tomato juice was co-fermented with multistrains, optimized by uniform experimental design and response surface methodology. Superoxide dismutase activity reached 496.67 U/g and lycopene content reached 77.12μg/g when P. pentosaceus (53.79%), L. casei (13.17%), L. plantarum (19.87%), L. fermentum (13.17%). To gain insight into the dynamics of metabolites during the tomato fermentation juice process multivariate statistical analysis was performed using the UHPLC-QE-MS/MS method...
2024: Frontiers in Nutrition
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