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https://www.readbyqxmd.com/read/28503801/pioppino-mushroom-in-the-southern-italy-an-undervalued-source-of-nutrients-and-bioactive-compounds
#1
Nicola Landi, Severina Pacifico, Sara Ragucci, Antonella M A Di Giuseppe, Federica Iannuzzi, Armando Zarrelli, Simona Piccolella, Antimo Di Maro
BACKGROUND: Agrocybe aegerita (V. Brig.) Singer, commonly known as Pioppino, is a popular edible mushroom, known in the Campania Region (Italy). Despite its habitual consumption, few nutritional and biochemical information is available. Thus, nutritional values, antiradical properties and chemical composition of the wild Pioppino were compared to those of the cultivated Agaricus bisporus (J.E. Lange) Imbach (known as Champignon), equally analysed. RESULTS: Macronutrient components (proteins, carbohydrates and lipids), free and protein amino acids and fatty acid content of poplar mushroom were achieved...
May 15, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28501026/metabolically-speaking-possible-reasons-behind-the-tolerance-of-sugar-belle-mandarin-hybrid-to-huanglongbing
#2
Nabil Killiny, Maria Filomena Valim, Shelley E Jones, Ahmad A Omar, Faraj Hijaz, Fred G Gmitter, Jude W Grosser
Huanglongbing (HLB) is currently considered the most destructive disease of citrus. Since its spread to the Americas, HLB has killed millions of trees and caused a sharp decline in production in many citrus growing regions. With the continuous spread of HLB disease in Florida and worldwide, there is an urgent need for the development of commercial citrus cultivars with a strong tolerance to HLB. Interestingly, field observations showed that some of the recently released mandarin hybrids such as 'Sugar Belle' were tolerant to HLB...
May 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28494096/physicochemical-properties-and-antioxidant-capacity-of-chinese-olive-canarium-album-l-cultivars
#3
Qiang Chang, Ming-Hua Su, Qing-Xi Chen, Bi-Yu Zeng, Hui-Hua Li, Wei Wang
In this study, the physicochemical characteristics and antioxidant potentials of 10 Chinese olive cultivars were investigated. Considerable differences were found between cultivars in weight, edible yield, water content, size, shape, total soluble solids, and total titratable acidity. The major sugars and organic acids in all the cultivars were glucose, fructose, and malic acid. Phenolics were the most abundant antioxidants (1174.0 to 1799.6 mg gallic acid equivalents/100 g fresh weight). Twelve phenolic compounds were identified and quantified by UPLC-MS/MS...
May 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28492135/ions-in-wine-and-their-relation-to-electrical-conductivity-under-ultrasound-irradiation
#4
Yan-Ying Yan, Qing-An Zhang, Er-Chun Li, Ya-Feng Zhang
Change in electrical conductivity is considered a potential indicator for the on-line monitoring of wine aging accelerated by ultrasound, as determined in our previous study; however, the exact mechanism of change is currently unclear. In this study, the ion content and the total ionic strength were analyzed by ion-exchange chromatography to investigate the change mechanism of the electrical conductivity of wine under ultrasound irradiation. The results indicate that the changes in wine electrical conductivity during ultrasound treatment correlate with the changes in the cations (Na(+), K(+), Ca(2+), Mg(2+), and NH₄(+)) and in the anions from the organic acids (malic acid, citric acid, tartaric acid, oxalic acid, and formic acid) and inorganic acids (Cl(-), SO₄(2-), and PO₄(3-)), especially for the ionic strength of the wine...
May 11, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28487539/mapk-mediated-auxin-signal-transduction-pathways-regulate-the-malic-acid-secretion-under-aluminum-stress-in-wheat-triticum-aestivum-l
#5
Xinwei Liu, Yameng Lin, Diqiu Liu, Chengxiao Wang, Zhuqing Zhao, Xiuming Cui, Ying Liu, Ye Yang
An isobaric tags for relative and absolute quantitative (iTRAQ)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (CK), aluminum (Al) and Al(+) indole-3-acetic acid (IAA) treatment of wheat lines ET8 (Al-tolerant). Further, the the expression levels of auxin response factor (ARF), Aux/IAA, Mitogen activated protein kinase (MAPK) 2c, and MAPK1a were analyzed. Results showed that 16 proteins were determined to be differentially expressed in response to Al and IAA co-treatment compared with Al alone...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28475173/microrna-30a-attenuates-mutant-kras-driven-colorectal-tumorigenesis-via-direct-suppression-of-me1
#6
Hongxing Shen, Chuan Xing, Kaisa Cui, Yunxiao Li, Jinxiang Zhang, Runlei Du, Xiaodong Zhang, Youjun Li
Frequent KRAS mutations contribute to multiple cancers including ~40% of human colorectal cancers (CRCs). Systematic screening of 1255 microRNAs (miRNAs) identified miR-30a as a synthetic lethal in KRAS-mutant CRC cells. miR-30a was downregulated in CRCs and repressed by P65. miR-30a directly targeted malic enzyme 1 (ME1) and KRAS, and inhibited anchorage-independent growth and in vivo tumorigenesis by KRAS-mutant CRC cells. ME1 was significantly upregulated in KRAS-mutant CRCs. Eliminating ME1 by short hairpin RNA (shRNA) resulted in obviously decreased NADPH production, levels of triglyceride and fatty acid, and an inhibition of tumorigenicity of KRAS-mutant CRCs...
May 5, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28472722/investigation-of-the-antimicrobial-effect-of-natural-deep-eutectic-solvents-nades-as-solvents-in-antimicrobial-photodynamic-therapy
#7
Kristine Opsvik Wikene, Håkon Valen Rukke, Ellen Bruzell, Hanne Hjorth Tønnesen
Natural deep eutectic solvents (NADES) are a third class of liquids, separate from water and lipids. Some NADES, especially those containing organic acids, have been suggested to possess antimicrobial properties. Such properties may be advantageous when NADES are used as solvents in e.g. antimicrobial photodynamic therapy. However, to control the toxicity of acid-containing NADES, they must retain their specific qualities upon dilution. Hence, the aims of this study were to investigate the effect of dilution on the acid-containing NADES network, their antimicrobial activity on different planktonic microorganisms, and their influence on phototoxicity when used as solvents for a photosensitiser...
April 27, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28471391/the-combined-use-of-schizosaccharomyces-pombe-and-lachancea-thermotolerans-effect-on-the-anthocyanin-wine-composition
#8
Ángel Benito, Fernando Calderón, Santiago Benito
The most popular methodology to make red wine is through the combined use of Saccharomyces cerevisiae yeast and lactic acid bacteria, for alcoholic fermentation and malolactic fermentation respectively. This classic winemaking practice produces stable red wines from a microbiological point of view. This study aims to investigate a recent red winemaking biotechnology, which through the combined use of Lachancea thermotolerans and Schizosaccharomyces pombe is used as an alternative to the classic malolactic fermentation...
May 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28469632/interplant-aboveground-signaling-prompts-upregulation-of-auxin-promoter-and-malate-transporter-as-part-of-defensive-response-in-the-neighboring-plants
#9
Connor Sweeney, Venkatachalam Lakshmanan, Harsh P Bais
When disrupted by stimuli such as herbivory, pathogenic infection, or mechanical wounding, plants secrete signals such as root exudates and volatile organic compounds (VOCs). The emission of VOCs induces a response in the neighboring plant communities and can improve plant fitness by alerting nearby plants of an impending threat and prompting them to alter their physiology for defensive purposes. In this study, we investigated the role of plant-derived signals, released as a result of mechanical wounding, that may play a role in intraspecific communication between Arabidopsis thaliana communities...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28460960/influence-of-processing-parameters-and-stress-adaptation-on-the-inactivation-of-listeria-monocytogenes-by-non-thermal-atmospheric-plasma-ntap
#10
Tamara Calvo, Avelino Álvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes López
This study evaluated the effectiveness of Non-Thermal Atmospheric Plasma (NTAP) treatments against Listeria. Firstly, the impact of gas composition and flow rate on L. monocytogenes and L. innocua (used as a surrogate) inactivation by NTAP was monitored. Secondly, the influence of stress adaptation (growth under suboptimal conditions, using a wide range of temperatures and media acidified up to pH5.5 with citric, lactic, malic or hydrochloric acid, or short-term exposure to acid, cold or thermal shocks) on L...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28455567/effect-of-low-molecular-weight-organic-acids-on-hematite-dissolution-promoted-by-desferrioxamine-b
#11
Qingqi Lin, Yingli Wang, Xiuhong Yang, Dishen Ruan, Shizhong Wang, Xiange Wei, Rongliang Qiu
Siderophores, as strong chelators specific to iron, have been intensively studied in relation to the facilitation of biological iron acquisition from iron oxides. In this study, the dissolution of hematite in the presence of the siderophore desferrioxamine B (DFOB) and three low-molecular-weight organic acids (LMWOAs, i.e., oxalic, citric, or malic acid) was investigated at three pH conditions (3.0, 5.5, and 9.0). Hematite dissolution was pH-dependent and LMWOA-specific. The adsorption of DFOB on hematite was significantly higher at pH 9...
April 28, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28455340/engineering-escherichia-coli-nicotinic-acid-mononucleotide-adenylyltransferase-for-fully-active-amidated-nad-biosynthesis
#12
Xueying Wang, Yongjin J Zhou, Lei Wang, Wujun Liu, Yuxue Liu, Chang Peng, Zongbao K Zhao
Nicotinamide adenine dinucleotide (NAD) and its reduced form NADH function as essential redox cofactors and have major roles in determining cellular metabolic features. NAD can be synthesized through deamidated and amidated pathway, of which the key reaction involves adenylylation of nicotinic acid mononucleotide (NaMN) and nicotinamide mononucleotide (NMN), respectively. In Escherichia coli, NAD de novo biosynthesis depends on the protein NadD-catalyzed adenylylation of NaMN to nicotinic acid adenine dinucleotide (NaAD), followed by NAD synthase-catalyzed amidation...
April 28, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28441480/metabolite-profiling-of-a-zinc-accumulating-rice-mutant
#13
Yin Wang, Sha Mei, Zhixue Wang, Zhoulei Jiang, Zhangshicang Zhu, Jingwen Ding, Dianxing Wu, Xiaoli Shu
Breeding crops with high zinc (Zn) density is an effective way to alleviate human dietary Zn deficiencies. We characterized a mutant Lilizhi (LLZ) accumulating at least 35% higher Zn concentration in grain than the wild type (WT) in hydroponic experiments. The mutant stored less Zn content in the root and transported more Zn to the grain. Metabolite profiling demonstrated that, with high Zn treatment, the contents of proline, asparagine, citric acid, and malic acid were enhanced in both LLZ and the WT, which were thought to be involved in Zn transport in rice...
May 2, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28433901/significantly-enhanced-enzymatic-hydrolysis-of-rice-straw-via-a-high-performance-two-stage-deep-eutectic-solvents-synergistic-pretreatment
#14
Xue-Dan Hou, Guo-Jian Feng, Mei Ye, Chao-Mei Huang, Yi Zhang
A two-stage deep eutectic solvents (DESs) treatment was shown to be an effective method for improving the utilization of certain DESs, and the specific order of pretreatment, such as malic acid/proline (MP) or choline chloride/oxalic acid (CO) during the first stage and choline chloride/urea (CU) during the second stage, resulted in better performance for enhancing the sugar yield due to the synergistic effect of the two DESs on biomass fractionation. Moreover, the presence of water during these processes could balance the loss of components by tuning the pretreatment severity, thus ensuring higher sugar yields...
April 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433582/effects-of-twenty-standard-amino-acids-on-biochemical-constituents-docosahexaenoic-acid-production-and-metabolic-activity-changes-of-crypthecodinium-cohnii
#15
Waseem Safdar, Xinyi Zan, Muhammad Shamoon, Hafiz Rizwan Sharif, Omar Mukama, Xin Tang, Yuanda Song
The influence of 20 standard amino acids was investigated on growth, lipid accumulation, docosahexaenoic acid (DHA) production and cell biochemical composition of Crypthecodinium cohnii. C. cohnii efficiently utilize organic nitrogen (predominantly threonine and to a lesser extent tyrosine and serine) as compared to inorganic nitrogen (NH4)2SO4. However, No significant effect was observed on major biochemical composition of C. cohnii (lipids, carbohydrates and proteins) under N limitation or supplementation with different N-sources...
April 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28429330/growth-kinetics-fatty-acid-composition-and-metabolic-activity-changes-of-crypthecodinium-cohnii-under-different-nitrogen-source-and-concentration
#16
Waseem Safdar, Muhammad Shamoon, Xinyi Zan, Junaid Haider, Hafiz Rizwan Sharif, Muhammad Shoaib, Yuanda Song
The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of lipids were accumulated in lipid accumulation stage (48-120 h) though, slow growth rate was observed during this stage. NaNO3 supported significantly higher lipid content (26.9% of DCW), DHA content (0...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28424673/genome-scale-reconstruction-of-the-metabolic-network-in-oenococcus-oeni-to-assess-wine-malolactic-fermentation
#17
Sebastián N Mendoza, Pablo M Cañón, Ángela Contreras, Magdalena Ribbeck, Eduardo Agosín
Oenococcus oeni is the main responsible agent for malolactic fermentation in wine, an unpredictable and erratic process in winemaking. To address this, we have constructed and exhaustively curated the first genome-scale metabolic model of Oenococcus oeni, comprising 660 reactions, 536 metabolites and 454 genes. In silico experiments revealed that nutritional requirements are predicted with an accuracy of 93%, while 14 amino acids were found to be essential for the growth of this bacterial species. When the model was applied to determine the non-growth associated maintenance, results showed that O...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28421045/cranberry-juice-and-combinations-of-its-organic-acids-are-effective-against-experimental-urinary-tract-infection
#18
Heidi D Jensen, Carsten Struve, Søren B Christensen, Karen A Krogfelt
The antibacterial effect of cranberry juice and the organic acids therein on infection by uropathogenic Escherichia coli was studied in an experimental mouse model of urinary tract infection (UTI). Reduced bacterial counts were found in the bladder (P < 0.01) of mice drinking fresh cranberry juice. Commercially available cranberry juice cocktail also significantly reduced (P < 0.01) bacterial populations in the bladder, as did the hydrophilic fraction of cranberry juice (P < 0.05). Quinic, malic, shikimic, and citric acid, the preponderant organic acids in cranberry juice, were tested in combination and individually...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28412312/updates-to-a-13-c-metabolic-flux-analysis-model-for-evaluating-energy-metabolism-in-cultured-cerebellar-granule-neurons-from-neonatal-rats
#19
Mika B Jekabsons, Hoda M Gebril, Yan-Hong Wang, Bharathi Avula, Ikhlas A Khan
A hexose phosphate recycling model previously developed to infer fluxes through the major glucose consuming pathways in cultured cerebellar granule neurons (CGNs) from neonatal rats metabolizing [1,2-(13)C2]glucose was revised by considering reverse flux through the non-oxidative pentose phosphate pathway (PPP) and symmetrical succinate oxidation within the tricarboxylic acid (TCA) cycle. The model adjusts three flux ratios to effect (13)C distribution in the hexose, pentose, and triose phosphate pools, and in TCA cycle malate to minimize the error between predicted and measured (13)C labeling in exported lactate (i...
April 12, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28408661/multiomics-of-tomato-glandular-trichomes-reveals-distinct-features-of-central-carbon-metabolism-supporting-high-productivity-of-specialized-metabolites
#20
Gerd Balcke, Stefan Bennewitz, Nick Bergau, Benedikt Athmer, Anja Henning, Petra Majovsky, José M Jiménez-Gómez, Wolfgang Hoehenwarter, Alain F Tissier
Glandular trichomes are metabolic cell factories with the capacity to produce large quantities of secondary metabolites. Little is known about the connection between central carbon metabolism and metabolic productivity for secondary metabolites in glandular trichomes. To address this gap in our knowledge, we performed comparative metabolomics, transcriptomics, proteomics and 13C-labeling of type VI glandular trichomes and leaves from a cultivated (Solanum lycopersicum LA4024) and a wild (Solanum habrochaites LA1777) tomato accession...
April 13, 2017: Plant Cell
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