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https://www.readbyqxmd.com/read/28229207/engineering-rtca-pathway-and-c4-dicarboxylate-transporter-for-l-malic-acid-production
#1
Xiulai Chen, Yuancai Wang, Xiaoxiang Dong, Guipeng Hu, Liming Liu
L-Malic acid is an important component of a vast array of food additives, antioxidants, disincrustants, pharmaceuticals, and cosmetics. Here, we presented a pathway optimization strategy and a transporter modification approach to reconstruct the L-malic acid biosynthesis pathway and transport system, respectively. First, pyruvate carboxylase (pyc) and malate dehydrogenase (mdh) from Aspergillus flavus and Rhizopus oryzae were combinatorially overexpressed to construct the reductive tricarboxylic acid (rTCA) pathway for L-malic acid biosynthesis...
February 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28221961/synergistic-antilisterial-effects-of-mixtures-of-lysozyme-and-organic-acids
#2
Myeonggeun Oh, Joongjae Lee, Yoonhwa Jeong, Misook Kim
We investigated the synergistic effects of lysozyme combined with organic acids to inhibit the growth of Listeria monocytogenes . The antilisterial effects of the combination of lysozyme and acetic acid, citric acid, lactic acid, malic acid, or succinic acid were evaluated using the checkerboard method and time-kill assay. The MIC was 25,000 mg/liter for lysozyme, 625 mg/liter for acetic acid, and 1,250 mg/liter for the other acids. The MBC was 10,000 mg/liter for all of the tested organic acids. The combination of lysozyme and each organic acid showed synergistic effects via the checkerboard method; however, the time-kill assay showed synergistic effects for only three combinations of 1,250 mg/liter lysozyme with succinic acid (312 and 625 mg/liter) or malic acid (625 mg/liter)...
December 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28199009/influence-of-different-yeast-lactic-acid-bacteria-combinations-on-the-aromatic-profile-of-red-bordeaux-wine
#3
Marine Gammacurta, Stéphanie Marchand, Virginie Moine, Gilles de Revel
BACKGROUND: The typical fruity aroma of red Bordeaux wines depends on the grape variety but also on microbiological processes, such as alcoholic and malolactic fermentations. These transformations involve respectively the yeast Saccharomyces cerevisiae and the lactic acid bacteria Oenococcus oeni. Both species play a central role in red winemaking but their quantitative and qualitative contribution to the revelation of the organoleptic qualities of wine has not yet been fully described...
February 15, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28193406/variation-of-ascorbic-acid-concentration-in-fruits-of-cultivated-and-wild-apples
#4
Ting Fang, Qiaoling Zhen, Liao Liao, Albert Owiti, Li Zhao, Schuyler S Korban, Yuepeng Han
Ascorbic acid (AsA) content in mature fruits of 457 apple accessions were measured, and a great variation in AsA concentration was detected. Wild fruits showed significantly higher level of AsA than cultivated fruits. Fruit AsA content was positively correlated with malic acid content, but negatively correlated with fruit weight and soluble solid content. Thus, the difference in AsA content between the wild and cultivated fruits could be attributed to an indirect consequence of human selection for larger fruit size, less acidity, and increased sweetness during apple domestication...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28191939/phytochemical-profiles-of-new-red-fleshed-apple-varieties-compared-with-traditional-and-new-white-fleshed-varieties
#5
David Bars-Cortina, Alba Macià, Ignasi Iglesias, Maria Paz Romero, Maria José Motilva
This study is an exhaustive chemical characterization of the phenolic compounds, triterpenes, and organic and ascorbic acids in red-fleshed apple varieties obtained by different breeding programs and using five traditional and new white-fleshed apple cultivars as reference. To carry out these analyses, solid-liquid extraction (SLE) and ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) were used. The results showed that the red-fleshed apples contained, in either the flesh or peel, higher amounts of phenolic acids (chlorogenic acid), anthocyanins (cyanidin-3-O-galactoside), dihydrochalcones (phloretin xylosyl glucoside), and organic acids (malic acid) but a lower amount of flavan-3-ols than the white-fleshed apples...
February 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28187228/effect-of-alprazolam-on-rat-serum-metabolic-profiles
#6
Yan Li, Gaotong Lin, Bingbao Chen, Jing Zhang, Lingtian Wang, Zixia Li, Yungang Cao, Congcong Wen, Xuezhi Yang, Gaozhong Cao, Xianqin Wang, Guoquan Cao
We developed a serum metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of alprazolam in rats. The GC-MS with HP-5MS (0.25 µm × 30 m × 0.25 mm), mass was conducted in EI mode with electron energy of 70 eV, full-scan mode with m/z 50-550. The rats were randomly divided to four group, three alprazolam treated group and control group. The alprazolam treated group rats were given 5, 10, 20 mg/kg (Low, Medium, High) of alprazolam by intragastric administration each day for 14 days, the serum samples were corrected on the seventh and fourteenth day for metabolomic study...
February 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28178638/fast-photoelectro-reduction-of-cr-vi-over-mos2-tio2-nanotubes-on-ti-wire
#7
Lixia Yang, Xutong Zheng, Ming Liu, Shenglian Luo, Yan Luo, Guifa Li
A stable MoS2 nanosheets@TiO2 NTAs composite was prepared via a simple hydrothermal process. Few-layer MoS2 nanosheets distributed on the TiO2 nanotube top surface and the inner walls rather than filling in the tubes, allowing abundant tubular channels open to environment and benefiting for efficient mass transport. Photocatalytic (PE) and photoelectrocatalytic (PEC) performance of the composite were evaluated on Cr(VI) reduction, with variable low molecule weight organic acids (LOAs) added as sacrificial electron donor to form a charge-transfer-complex (CTC) between LOAs and TiO2/MoS2, which is sensitive to the visible light illumination and could induce the photo-reduction of Cr(VI) and photo-oxidation of LOAs...
January 30, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28145808/characterization-of-the-l-glutamate-clearance-pathways-across-the-blood-brain-barrier-and-the-effect-of-astrocytes-in-an-in%C3%A2-vitro-blood-brain-barrier-model
#8
Hans Cc Helms, Blanca I Aldana, Simon Groth, Morten M Jensen, Helle S Waagepetersen, Carsten U Nielsen, Birger Brodin
The aim was to characterize the clearance pathways for L-glutamate from the brain interstitial fluid across the blood-brain barrier using a primary in vitro bovine endothelial/rat astrocyte co-culture. Transporter profiling was performed using uptake studies of radiolabeled L-glutamate with co-application of transporter inhibitors and competing amino acids. Endothelial abluminal L-glutamate uptake was almost abolished by co-application of an EAAT-1 specific inhibitor, whereas luminal uptake was inhibited by L-glutamate and L-aspartate (1 mM)...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28140733/integrated-metabonomic-proteomic-studies-on-blood-enrichment-effects-of-angelica-sinensis-on-a-blood-deficiency-mice-model
#9
Yongli Hua, Wangling Yao, Peng Ji, Yanming Wei
CONTEXT: Angelica sinensis (Oliv.) Diels (Umbelliferae) (AS) is a well-known Traditional Chinese Medicine (TCM) that enriches and regulates the blood. OBJECTIVE: An integrated metabonomic and proteomic method was developed and applied to study the blood enrichment effects and mechanisms of AS on blood deficiency (BD) mouse model. MATERIALS AND METHODS: Forty mice were randomly divided into the control, BD, High-dose of AS (ASH), Middle-dose of AS (ASM), and Low-dose of AS (ASL) groups...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28139228/chiral-separation-of-short-chain-aliphatic-hydroxycarboxylic-acids-on-cinchonan-carbamate-based-weak-chiral-anion-exchangers-and-zwitterionic-chiral-ion-exchangers
#10
Carlos Calderón, Michael Lämmerhofer
Chiral short chain aliphatic hydrocarboxylic acids (HCAs) are common compounds being part of different biological processes. In order to control and understand these processes is of pivotal importance to determine the identity of the involved enantiomer or their enantiomeric ratio. In this study the capacity of quinine- and quinidine-derived chiral stationary phases to perform the enantioseparation of eight chiral HCAs (tartaric acid, isocitric acid, malic acid, glyceric acid, 2-hydroxyglutaric acid, 2-hydroxybutyric acid, lactic acid and 3-hydroxybutyric acid) was evaluated...
March 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28101749/twenty-seven-years-of-cerebral-pyruvate-recycling
#11
Sebastián Cerdán
Cerebral pyruvate recycling is a metabolic pathway deriving carbon skeletons and reducing equivalents from mitochondrial oxaloacetate and malate, to the synthesis of mitochondrial and cytosolic pyruvate, lactate and alanine. The pathway allows both, to provide the tricarboxylic acid cycle with pyruvate molecules produced from alternative substrates to glucose and, to generate reducing equivalents necessary for the operation of NADPH requiring processes. At the cellular level, pyruvate recycling involves the activity of malic enzyme, or the combined activities of phosphoenolpyruvate carboxykinase and pyruvate kinase, as well as of those transporters of the inner mitochondrial membrane exchanging the corresponding intermediates...
January 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28099419/genomic-deletion-of-malic-enzyme-2-confers-collateral-lethality-in-pancreatic-cancer
#12
Prasenjit Dey, Joelle Baddour, Florian Muller, Chia Chin Wu, Huamin Wang, Wen-Ting Liao, Zangdao Lan, Alina Chen, Tony Gutschner, Yaan Kang, Jason Fleming, Nikunj Satani, Di Zhao, Abhinav Achreja, Lifeng Yang, Jiyoon Lee, Edward Chang, Giannicola Genovese, Andrea Viale, Haoqiang Ying, Giulio Draetta, Anirban Maitra, Y Alan Wang, Deepak Nagrath, Ronald A DePinho
The genome of pancreatic ductal adenocarcinoma (PDAC) frequently contains deletions of tumour suppressor gene loci, most notably SMAD4, which is homozygously deleted in nearly one-third of cases. As loss of neighbouring housekeeping genes can confer collateral lethality, we sought to determine whether loss of the metabolic gene malic enzyme 2 (ME2) in the SMAD4 locus would create cancer-specific metabolic vulnerability upon targeting of its paralogous isoform ME3. The mitochondrial malic enzymes (ME2 and ME3) are oxidative decarboxylases that catalyse the conversion of malate to pyruvate and are essential for NADPH regeneration and reactive oxygen species homeostasis...
February 2, 2017: Nature
https://www.readbyqxmd.com/read/28092854/biopharmaceutical-characterization-of-praziquantel-cocrystals-and-cyclodextrin-complexes-prepared-by-grinding
#13
Martina Cugovčan, Jasna Jablan, Jasmina Lovrić, Dominik Cinčić, Nives Galić, Mario Jug
Mechanochemical activation using several different co-grinding additives was applied as a green chemistry approach to improve physiochemical and biopharmaceutical properties of praziquantel (PZQ). Liquid assisted grinding with an equimolar amount of citric acid (CA), malic acid (MA), salicylic acid (SA) and tartaric acid (TA) gained in cocrystal formation, which all showed pH-dependent solubility and dissolution rate. However, the most soluble cocrystal of PZQ with MA was chemically unstable, as seen during the stability testing...
January 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28071001/purified-isolation-of-vacuoles-from-sedum-alfredii-leaf-derived-protoplasts
#14
Xiao-Yu Gao, Xing-Cheng Liao, Ruo-Lai Wu, Ting Liu, Hai-Xing Wang, Ling-Li Lu
This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macerozyme at 50 °C for 5 min significantly accelerated the cell wall degradation. For the most optimal conditions for mesophyll protoplast isolation, the mixture of fresh leaves and cell lysates was followed by a 2-h-long vibration. The protoplast lysate for vacuole isolation was diluted, and 0.675 mmol/L was identified as the most appropriate 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonic acid (CHAPS) level, in which S...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28057501/cloning-and-characterization-of-pyruvate-carboxylase-gene-responsible-for-calcium-malate-overproduction-in-penicillium-viticola-152-and-its-expression-analysis
#15
Ibrar Khan, Sadia Qayyum, Shehzad Ahmed, Farhana Maqbool, Isfahan Tauseef, Kashif Syed Haleem, Zhen-Ming Chi
In this study, a pyruvate carboxylase gene (PYC) from a marine fungus Penicillium viticola 152 isolated from marine algae was cloned and characterized by using Genome Walking method. An open reading frame (ORF) of The PYC gene (accession number: KM593097) had 3582bp encoding 1193 amino acid protein (isoelectric point: 5.01) with a calculated molecular weight of 131.2757kDa. A putative promoter (intronless) of the gene was located at -666bp and contained a TATA box, several CAAT boxes, the 5'-SYGGRG-3' and a 5'-HGATAR-3' sequences...
March 20, 2017: Gene
https://www.readbyqxmd.com/read/28054502/enhanced-ph-stability-cell-viability-and-reduced-degradation-rate-of-poly-l-lactide-based-composite-in-vitro-effect-of-modified-magnesium-oxide-nanoparticles
#16
Jinjun Yang, Xiuxiang Cao, Yun Zhao, Liang Wang, Bei Liu, Junping Jia, Hui Liang, Minfang Chen
The modified MgO nanoparticles (m-MgO-NPs) by a copolymer containing the malic acid and low molecular weight poly(L-lactide) (poly(L-lactide-co-malic acid), PLMA) have been successfully prepared. MgO nanoparticles (MgO-NPs) were coated by the PLMA and m-MgO-NPs were uniformly dispersed in the PLLA matrix to a novel biocomposite material (PLLA/m-MgO-NPs) with more excellent interface bonding and uniformer dispersion, compared to the PLLA/MgO-NPs. Compared to neat PLLA and PLLA/MgO-NPs film, the m-MgO-NPs not only shown the obvious neutralization effect on the acidic solution in the degradation of the PLLA and better hydrophilicity, but also exhibited the higher cell viability and decrease the toxicity to the cell in the degradation process of PLLA in vitro...
January 16, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28050761/role-of-the-polycarboxylic-compounds-in-the-response-of-silene-vulgaris-to-chromium
#17
Ana E Pradas Del Real, Jose Manuel Silvan, Sonia de Pascual-Teresa, Ana Guerrero, Pilar García-Gonzalo, M Carmen Lobo, Araceli Pérez-Sanz
This work aims to investigate the nature and the specific mechanisms by which polycarboxylic compounds participate in the tolerance of Silene vulgaris to Cr with special attention given to the rhizosphere system. This knowledge is important to use this species in the implementation of phytoremediation technologies in Cr-polluted soils. According to the results, chromium is chelated and mobilized by the citric and malic acids in plant tissues, while oxalic acid might participate in the reduction and chelation of Cr in the rhizosphere...
January 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28043922/reconstruction-of-a-genome-scale-metabolic-model-and-in-silico-analysis-of-the-polymalic-acid-producer-aureobasidium-pullulans-cctcc-m2012223
#18
Jun Feng, Jing Yang, Xiaorong Li, Meijin Guo, Bochu Wang, Shang-Tian Yang, Xiang Zou
Aureobasidium pullulans is a yeast-like fungus used for producing biopolymers e.g. polymalic acid (PMA) and pullulan. A high PMA producing strain, A. pullulans CCTCC M2012223, was isolated and sequenced in our previous study. To understand its metabolic performance, a genome-scale metabolic model, iZX637, consisting of 637 genes, 1347 reactions and 1133 metabolites, was reconstructed based on genome annotation and literature mining studies. The iZX637 model was validated by simulating cell growth, utilization of carbon and nitrogen sources, and gene essentiality analysis in A...
April 5, 2017: Gene
https://www.readbyqxmd.com/read/28042988/effect-of-fulvic-acid-induction-on-the-physiology-metabolism-and-lipid-biosynthesis-related-gene-transcription-of-monoraphidium-sp-fxy-10
#19
Raoqiong Che, Li Huang, Jun-Wei Xu, Peng Zhao, Tao Li, Huixian Ma, Xuya Yu
Fulvic acid (FA) triggers lipid accumulation in Monoraphidium sp. FXY-10, which can produce biofuels. Therefore, the metabolism shift and gene expression changes influenced by fulvic acid should be investigated. In this study, lipid and protein contents increased rapidly from 44.6% to 54.3% and from 31.4% to 39.7% under FA treatment, respectively. By contrast, carbohydrate content sharply declined from 49.5% to 32.5%. The correlation between lipid content and gene expression was also analyzed. Results revealed that accD, ME, and GPAT genes were significantly correlated with lipid accumulation...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28040580/physiological-effects-of-%C3%AE-linolenic-acid-and-sesamin-on-hepatic-fatty-acid-synthesis-and-oxidation
#20
Takashi Ide, Haruka Iwase, Saaya Amano, Saki Sunahara, Ayuka Tachihara, Minako Yagi, Tsuyoshi Watanabe
Interrelated effects of γ-linolenic acid (GLA) and sesamin, a sesame lignan, on hepatic fatty acid synthesis and oxidation were examined. Rats were fed experimental diets supplemented with 0 or 2 g/kg sesamin (1:1 mixture of sesamin and episesamin) and containing 100 g/kg of palm oil (saturated fat), safflower oil rich in linoleic acid, or oil of evening primrose origin containing 43% GLA (GLA oil) for 18 days. In rats fed sesamin-free diets, GLA oil, compared with other oils, increased the activity and mRNA levels of various enzymes involved in fatty acid oxidation, except for some instances...
December 14, 2016: Journal of Nutritional Biochemistry
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