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https://www.readbyqxmd.com/read/28727044/muscle-lipid-metabolism-in-two-rabbit-lines-divergently-selected-for-intramuscular-fat
#1
M Martínez-Álvaro, S Agha, A Blasco, P Hernández
A divergent selection experiment for intramuscular fat (IMF) of LM at 9 wk of age was performed in rabbits. The objective of this work was to compare the lipid metabolism in muscles and fat tissues of the high-IMF and low-IMF lines. Lipogenic, catabolic, and lipolytic activities were studied in 2 muscles with different oxidative patterns (LM and semimembranosus proprius) and in the perirenal fat depot at 2 ages, 9 and 13 wk. In addition, adipocytes were characterized in perirenal fat. In the fifth generation, direct response to selection was 0...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28716425/characterization-and-quantification-of-flavonoids-and-organic-acids-over-fruit-development-in-american-cranberry-vaccinium-macrocarpon-cultivars-using-hplc-and-apci-ms-ms
#2
Yifei Wang, Jennifer Johnson-Cicalese, Ajay P Singh, Nicholi Vorsa
Cranberry flavonoids, including anthocyanins, flavonol glycosides and proanthocyanidins, and organic acids were characterized and quantified by HPLC and LC-MS/MS during fruit development and ripening in eight cranberry cultivars. Anthocyanin biosynthesis initiated at early fruit development and reached highest level in mature fruit, with significant differences between cultivars. Major flavonol glycosides, including the most abundant quercetin-3-galactoside and myricetin-3-galactoside, showed consistent concentrations during the season with moderate fluctuation, and were at similar levels in mature fruits of the eight cultivars...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28711996/the-physiological-and-metabolic-changes-in-sugar-beet-seedlings-under-different-levels-of-salt-stress
#3
Yuguang Wang, Piergiorgio Stevanato, Lihua Yu, Huijie Zhao, Xuewei Sun, Fei Sun, Jing Li, Gui Geng
Salinity stress is a major limitation to global crop production. Sugar beet, one of the world's leading sugar crops, has stronger salt tolerant characteristics than other crops. To investigate the response to different levels of salt stress, sugar beet was grown hydroponically under 3 (control), 70, 140, 210 and 280 mM NaCl conditions. We found no differences in dry weight of the aerial part and leaf area between 70 mM NaCl and control conditions, although dry weight of the root and whole plant treated with 70 mM NaCl was lower than control seedlings...
July 15, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28710744/cloning-and-characterization-of-f3pyc-gene-encoding-pyruvate-carboxylase-in-aspergillus-flavus-strain-f3
#4
Sadia Qayyum, Ibrar Khan, Zulfiqar Ahmad Bhatti, Changsheng Peng
Pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and L-malic acid. These organic acids play very important role for biological remediation of heavy metals. In this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (F3PYC) from heavy metal resistant indigenous fungal isolate Aspergillus flavus (F3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a calculated molecular weight of 131...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28699758/resilience-of-malic-acid-natural-deep-eutectic-solvent-nanostructure-to-solidification-and-hydration
#5
Oliver S Hammond, Daniel Timothy Bowron, Andrew J Jackson, Thomas Arnold, Adrian Sanchez-Fernandez, Nikolaos Tsapatsaris, Victoria Garcia Sakai, Karen J Edler
Little is presently known about the unique nanostructure of Deep Eutectic Solvents (DES). The order of the liquid-solid phase transition is contended and whether DES-water mixtures are merely aqueous solutions, or have properties dominated by the eutectic pair, is unclear. Here, we unambiguously show the structure of choline chloride-malic acid (malicine) as a liquid, and also in solid and hydrated forms, using neutron total scattering on D/H isotope-substituted samples, and quasi-elastic neutron scattering (QENS)...
July 12, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28697384/schizosaccharomyces-pombe-and-saccharomyces-cerevisiae-yeasts-in-sequential-fermentations-effect-on-phenolic-acids-of-fermented-kei-apple-dovyalis-caffra-l-juice
#6
P P Minnaar, N P Jolly, V Paulsen, H W Du Plessis, M Van Der Rijst
Kei-apple (Dovyalis caffra) is an evergreen tree indigenous to Southern Africa. The fruit contains high concentrations of l-malic acid, ascorbic acid, and phenolic acids. Kei-apple juice was sequentially inoculated with Schizosaccharomyces pombe and Saccharomyces cerevisiae yeasts. A reference fermentation using only S. cerevisiae was included. The fermentation was monitored by recording mass loss. At the end of fermentation, twelve untrained judges conducted free choice aroma profiling on the fruit wines. The Kei-apple juice and wines were analysed for total titratable acidity, total soluble solids, pH, alcohol, l-malic acid, and phenolic acids...
July 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28695065/-13-c-based-proteinogenic-amino-acid-paa-and-metabolic-flux-ratio-analysis-of-lactococcus-lactis-reveals-changes-in-pentose-phosphate-pp-pathway-in-response-to-agitation-and-temperature-related-stresses
#7
Kamalrul Azlan Azizan, Habtom W Ressom, Eduardo R Mendoza, Syarul Nataqain Baharum
Lactococcus lactis subsp. cremoris MG1363 is an important starter culture for dairy fermentation. During industrial fermentations, L. lactis is constantly exposed to stresses that affect the growth and performance of the bacterium. Although the response of L. lactis to several stresses has been described, the adaptation mechanisms at the level of in vivo fluxes have seldom been described. To gain insights into cellular metabolism, (13)C metabolic flux analysis and gas chromatography mass spectrometry (GC-MS) were used to measure the flux ratios of active pathways in the central metabolism of L...
2017: PeerJ
https://www.readbyqxmd.com/read/28690628/comparison-of-salt-tolerance-in-soja-based-on-metabolomics-of-seedling-roots
#8
Mingxia Li, Rui Guo, Yang Jiao, Xiaofei Jin, Haiyan Zhang, Lianxuan Shi
Soybean is an important economic crop that is continually threatened by abiotic stresses, especially salt stress. Wild soybean is an important germplasm resource for the breeding of cultivated soybean. The root system plays a very important role in plant salt tolerance. To explore the salt tolerance-related mechanisms among Soja, we have demonstrated the seedling roots' growth and metabolomics in wild soybean, semi-wild soybean, and cultivated soybean under two types of salt stress by using gas chromatography-mass spectrometry...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28674927/influence-of-glycosides-on-behavior-of-oenococcus-oeni-in-wine-conditions-growth-substrates-and-aroma-compounds
#9
Carmen Maturano, Fabiana María Saguir
Autochthonous Oenococcus oeni strains (MS9, MS20 and MS46) with good malolactic performance and yielding adequate diacetyl levels, were selected to investigate the effect of synthetic and grape glycosides on bacterial growth, substrate utilization and β-glucosidase (βGlu), α-arabinofuranosidase (αAra) and α-rhamnopyranosidase (αRha) activities in a wine-like medium containing 6% ethanol, pH 4.0 (WBM). Then, changes in the volatile compounds profile were evaluated at the end of malolactic fermentation (MLF) carried out by the MS46 strain in WBM containing 1 mg L(-1) of natural glycoside...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28671281/water-status-and-must-composition-in-grapevine-cv-chardonnay-with-different-soils-and-topography-and-a-mini-meta-analysis-of-the-%C3%AE-13-c-water-potentials-correlation
#10
Luca Brillante, Olivier Mathieu, Jean Lévêque, Cornelis van Leeuwen, Benjamin Bois
BACKGROUND: The measurement of carbon isotopic discrimination in grape sugars at harvest (δ(13) C) is an integrated assessment of water status during ripening. It is an efficient alternative to assess variability in the field and discriminate between management zones in precision viticulture, but further work is needed to completely understand the signal. RESULTS: This work, spanning over three years, performed in a hillslope toposequence in Burgundy, delineates the relationships between main soil properties (gravel amount, slope, texture) and the grapevine water status assessed by δ(13) C...
July 3, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28655216/phenotypic-and-genomic-survey-on-organic-acid-utilization-profile-of-pseudomonas-mendocina-strain-s5-2-a-vineyard-soil-isolate
#11
Teik Min Chong, Jian-Woon Chen, Wah-Seng See-Too, Choo-Yee Yu, Geik-Yong Ang, Yan Lue Lim, Wai-Fong Yin, Catherine Grandclément, Denis Faure, Yves Dessaux, Kok-Gan Chan
Root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. The exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. Therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. In this study, a comprehensive survey on the utilization of grapevine and rootstock related organic acids were conducted on a vineyard soil isolate which is Pseudomonas mendocina strain S5...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28638469/dual-ph-sensitive-charge-reversal-nanocomplex-for-tumor-targeted-drug-delivery-with-enhanced-anticancer-activity
#12
Qing Zhou, Yilin Hou, Li Zhang, Jianlin Wang, Youbei Qiao, Songyan Guo, Li Fan, Tiehong Yang, Lin Zhu, Hong Wu
Poly(β-L-malic acid) (PMLA), a natural aliphatic polyester, has been proven to be a promising carrier for anti-cancer drugs. In spite of excellent bio-compatibility, the application of PMLA as the drug carrier for cancer therapy is limited by its low cellular uptake efficiency. The strong negative charge of PMLA impedes its uptake by cancer cells because of the electrostatic repulsion. In this study, a dual pH-sensitive charge-reversal PMLA-based nanocomplex (PMLA-PEI-DOX-TAT@PEG-DMMA) was developed for effective tumor-targeted drug delivery, enhanced cellular uptake, and intracellular drug release...
2017: Theranostics
https://www.readbyqxmd.com/read/28631237/structural-characterization-of-pyoverdines-produced-by-pseudomonas-putida-kt2440-and-pseudomonas-taiwanensis-vlb120
#13
Matthias Baune, Yulin Qi, Karen Scholz, Dietrich A Volmer, Heiko Hayen
The previously unknown sequences of several pyoverdines (PVD) produced by a biotechnologically-relevant bacterium, namely, Pseudomonas taiwanensis VLB120, were characterized by high performance liquid chromatography (HPLC)-high resolution mass spectrometry (HRMS). The same structural characterization scheme was checked before by analysis of Pseudomonas sp. putida KT2440 samples with known PVDs. A new sample preparation strategy based on solid-phase extraction was developed, requiring significantly reduced sample material as compared to existing methods...
June 19, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28612355/effect-of-particle-size-on-phytochemical-composition-and-antioxidant-properties-of-two-persimmon-flours-from-diospyros-kaki-thunb-vars-rojo-brillante-and-triumph-coproducts
#14
Raquel Lucas-González, Juana Fernández-López, José Ángel Pérez-Álvarez, Manuel Viuda-Martos
BACKGROUND: This contribution studies the influence of the particle size of persimmon flours (from two cultivars, 'Rojo Brillante' and 'Triumph') on their primary (sugars and organic acids) and secondary (polyphenols, flavonoids and carotenoids) metabolite content, and also on their antioxidant activity, to assess whether these flours could find applications in the food industry as a potential functional ingredient. RESULTS: The main sugars were fructose and glucose and the principal organic acid was malic acid...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28612342/effect-of-glycine-nitrogen-on-lettuce-growth-under-soilless-culture-a-metabolomics-approach-to-identify-the-main-changes-occurred-in-plant-primary-and-secondary-metabolism
#15
Xiao Yang, Lei Feng, Li Zhao, Xiaosong Liu, Danial Hassani, Danfeng Huang
BACKGROUND: Lettuce is a significant source of antioxidants and bioactive compounds. Nitrate is a cardinal fertilizer in horticulture and influences vegetable yield and quality; however, the negative effects of nitrate on the biosynthesis of flavonoids require further study. It is expected that using fertilizers containing organic N could promote the synthesis of health-promoting compounds. RESULTS: Lettuces were hydroponically cultured in media containing 9 mM nitrate or 9 mM glycine for 4 weeks...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28601082/thiamine-induces-long-term-changes-in-amino-acid-profiles-and-activities-of-2-oxoglutarate-and-2-oxoadipate-dehydrogenases-in-rat-brain
#16
P M Tsepkova, A V Artiukhov, A I Boyko, V A Aleshin, G V Mkrtchyan, M A Zvyagintseva, S I Ryabov, A L Ksenofontov, L A Baratova, A V Graf, V I Bunik
Molecular mechanisms of long-term changes in brain metabolism after thiamine administration (single i.p. injection, 400 mg/kg) were investigated. Protocols for discrimination of the activities of the thiamine diphosphate (ThDP)-dependent 2-oxoglutarate and 2-oxoadipate dehydrogenases were developed to characterize specific regulation of the multienzyme complexes of the 2-oxoglutarate (OGDHC) and 2-oxoadipate (OADHC) dehydrogenases by thiamine. The thiamine-induced changes depended on the brain-region-specific expression of the ThDP-dependent dehydrogenases...
June 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28596074/introduction-of-an-acetyl-coa-carboxylation-bypass-into-escherichia-coli-for-enhanced-free-fatty-acid-production
#17
Kwang Soo Shin, Sung Kuk Lee
This study investigated the effect of the methylmalonyl-CoA carboxyltransferase (MMC) of Propionibacterium freudenreichii on production of free fatty acid (FFA) in Escherichia coli. Overexpression of the MMC exhibited a 44% increase in FFA titer. Co-overexpression of MMC and phosphoenolpyruvate carboxylase (PPC), which supplies the MMC precursor, further improved the titer by 40%. Expression of malic enzyme (MaeB) led to a 23% increase in FFA titer in the acetyl-CoA carboxylase (ACC)-overexpressing cells, but no increase in the MMC-overexpressing cells...
May 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28594267/a-plant-pathogenic-bacterium-exploits-the-tricarboxylic-acid-cycle-metabolic-pathway-of-its-insect-vector
#18
Nabil Killiny, Yasser Nehela, Faraj Hijaz, Christopher I Vincent
Huanglongbing in citrus is caused by a phloem-limited, uncultivable, gram-negative α-proteobacterium, Candidatus Liberibacter asiaticus (CLas). CLas is transmitted by the phloem-sucking insect, Diaphorina citri (Hemiptera: Liviidae), in a persistent, circulative, and propagative manner. In this study, we investigated the metabolomic and respiration rates changes in D. citri upon infection with CLas using gas chromatography-mass spectrometry (GC-MS) and gas exchange analysis. The level of glycine, L-serine, L-threonine, and gamma-amino butyric acid were higher in CLas-infected D...
June 8, 2017: Virulence
https://www.readbyqxmd.com/read/28591430/qualitative-hplc-dad-esi-tof-ms-analysis-cytotoxic-and-apoptotic-effects-of-croatian-endemic-centaurea-ragusina-l-aqueous-extracts
#19
Mila Radan, Ivana Carev, Vele Tešević, Olivera Politeo, Vedrana Čikeš Čulić
Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD-ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A-3, 13 diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin...
June 7, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28581736/highly-efficient-malolactic-fermentation-of-red-wine-using-encapsulated-bacteria-in-a-robust-biocomposite-of-silica-alginate
#20
Guillermo Simó, Josefina Vila-Crespo, Encarnación Fernández-Fernández, Violeta Ruipérez, José Manuel Rodríguez-Nogales
Bacteria encapsulation to develop malolactic fermentation emerges as a biotechnological strategy that provides significant advantages over the use of free cells. Two encapsulation methods have been proposed embedding Oenococcus oeni, (i) interpenetrated polymer networks of silica and Ca-alginate and (ii) Ca-alginate capsules coated with hydrolyzed 3-aminopropyltriethoxysilane (hAPTES). On the basis of our results, only the first method was suitable for bacteria encapsulation. The optimized silica-alginate capsules exhibited a negligible bacteria release and an increase of 328% and 65% in L-malic acid consumption and mechanical robustness, respectively, compared to untreated alginate capsules...
June 28, 2017: Journal of Agricultural and Food Chemistry
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