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https://www.readbyqxmd.com/read/28101518/is-it-time-to-include-ct-reverse-halo-sign-and-qpcr-targeting-mucorales-in-serum-to-eortc-msg-criteria-for-the-diagnosis-of-pulmonary-mucormycosis-in-leukemia-patients
#1
Denis Caillot, Stéphane Valot, Ingrid Lafon, Louise Basmaciyan, Marie Lorraine Chretien, Marc Sautour, Laurence Million, Caroline Legouge, Alexandre Payssot, Frédéric Dalle
In 23 leukemia patients with proven (n = 17) or possible (n = 6) pulmonary mucormycosis (PM), the presence of reversed halo sign on computed tomography was strongly associated with the positivity of quantitative polymerase chain reaction assays targeting Mucorales in the serum, confirming the value of these two tools for the diagnosis of PM in this setting.
October 2016: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28087338/auditory-hindbrain-atrophy-and-anomalous-calcium-binding-protein-expression-after-neonatal-exposure-to-monosodium-glutamate
#2
Lindsey Foran, Kaitlyn Blackburn, Randy J Kulesza
Glutamate is the most abundant excitatory neurotransmitter in the central nervous system, and is stored and released by both neurons and astrocytes. Despite the important role of glutamate as a neurotransmitter, elevated extracellular glutamate can result in excitotoxicity and apoptosis. Monosodium glutamate (MSG) is a naturally occurring sodium salt of glutamic acid that is used as a flavor enhancer in many processed foods. Previous studies have shown that MSG administration during the early postnatal period results in neurodegenerative changes in several forebrain regions, characterized by neuronal loss and neuroendocrine abnormalities...
January 10, 2017: Neuroscience
https://www.readbyqxmd.com/read/28077281/does-acute-or-habitual-protein-deprivation-influence-liking-for-monosodium-glutamate
#3
Una Masic, Martin R Yeomans
The umami flavour generated by monosodium glutamate (MSG) has been proposed as the marker for the presence of protein in foods. As protein is the most closely regulated macronutrient in the diet, the present study addressed whether acute protein deprivation, habitual protein intake or a combination of the two influenced liking for the taste of MSG. 24 low-restraint male participants (mean age: 22; BMI: 23) consumed either their habitual breakfast (baseline), a low protein breakfast (breakfast meal with low protein milk and milkshake) or a high protein breakfast (breakfast meal with high protein milk and milkshake) on three different days, and then evaluated the acceptability of umami (MSG), salty (NaCl) or sweet (Acesulphame K) tastes at low or high concentrations in a soup context at lunchtime...
January 7, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28058453/early-history-of-the-breeding-of-amino-acid-producing-strains
#4
Shigeru Nakamori
Amino acid production started in Japan in 1908 with the extraction of monosodium glutamate (MSG) from acid hydrolysates of proteins. In addition to extraction, other methods of amino acid production include chemical synthesis, fermentation, and enzymatic synthesis both for glutamic acid and other amino acids. In this chapter, we review the historical transition of these production methods; currently, fermentation is the chief production method of amino acids. All wild-type microorganisms possess a negative feedback control system (feedback inhibition and repression) on the enzymes within the amino acid biosynthetic pathways...
January 6, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28055264/inos-inhibition-improves-autonomic-dysfunction-and-oxidative-status-in-hypertensive-obese-rats
#5
Natalia Veronez da Cunha, Fernanda Novi Cortegoso Lopes, Carolina Panis, Rubens Cecchini, Phileno Pinge-Filho, Marli Cardoso Martins-Pinge
It has been suggested that nitric oxide (NO) from iNOS source is involved in inflammation and oxidative stress, and hypertension in obese subjects involves an inflammatory process. However, no study evaluated the participation of iNOS inhibition on cardiovascular, autonomic, and inflammatory parameters in obese rats. Obesity was induced by the administration of 4 mg/g body weight of monosodium glutamate (MSG) or equimolar saline (CTR) in newborn rats. On the 60th day, treatment with aminoguanidine (Amino, 50 mg/kg), an iNOS inhibitor, or 0...
2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28040184/effect-of-osmotic-dehydration-of-olives-as-pre-fermentation-treatment-and-partial-substitution-of-sodium-chloride-by-monosodium-glutamate-in-the-fermentation-profile-of-kalamata-natural-black-olives
#6
Stamatoula Bonatsou, Vasilis Iliopoulos, Athanasios Mallouchos, Eleni Gogou, Vasiliki Oikonomopoulou, Magdalini Krokida, Petros Taoukis, Efstathios Z Panagou
This study examined the effect of osmotic dehydration of Kalamata natural black olives as pre-fermentation treatment in combination with partial substitution of NaCl by monosodium glutamate (MSG) on the fermentation profile of olives. Osmotic dehydration was undertaken by immersing the olives in 70% (w/w) glucose syrup overnight at room temperature. Further on, three different mixtures of NaCl and MSG with/without prior osmotic dehydration of olives were investigated, namely (i) 6.65% NaCl - 0.35% MSG (5% substitution), (ii) 6...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28004032/histological-and-metabolic-state-of-dams-suckling-small-litter-or-msg-treated-pups
#7
Claudia Regina Capriglioni Cancian, Nayara Carvalho Leite, Elisangela Gueiber Montes, Stefani Valeria Fisher, Leticia Waselcoski, Emily Caroline Lopes Stal, Renata Zanardini Christoforo, Sabrina Grassiolli
Lactation is an important function that is dependent on changes in the maternal homeostasis and sustained by histological maternal adjustments. We evaluated how offspring manipulations during the lactational phase can modulate maternal morphologic aspects in the mammary gland, adipose tissue, and pancreatic islets of lactating dams. Two different models of litter-manipulation-during-lactation were used: litter sizes, small litters (SL) or normal litters (NL) and subcutaneous injections in the puppies of monosodium glutamate (MSG), or saline (CON)...
2016: TheScientificWorldJournal
https://www.readbyqxmd.com/read/27989379/epidemiology-of-invasive-aspergillosis-and-azole-resistance-in-patients-with-acute-leukaemia-the-sepia-study
#8
Philipp Koehler, Axel Hamprecht, Oliver Bader, Isabelle Bekeredjian-Ding, Dieter Buchheidt, Gottfried Doelken, Johannes Elias, Gerhard Haase, Corinna Hahn-Ast, Meinolf Karthaus, Alexander Kekulé, Peter Keller, Michael Kiehl, Stefan W Krause, Carolin Krämer, Silke Neumann, Holger Rohde, Paul La Rosée, Markus Ruhnke, Philippe Schafhausen, Enrico Schalk, Katrin Schulz, Stefan Schwartz, Gerda Silling, Peter Staib, Andrew Ullmann, Maria Vergoulidou, Thomas Weber, Oliver A Cornely, Maria J G T Vehreschild
Invasive aspergillosis (IA) is a serious hazard to high-risk haematological patients. There are increasing reports of azole-resistant Aspergillus spp. This study assessed the epidemiology of IA and azole-resistant Aspergillus spp. in patients with acute leukaemia in Germany. A prospective multicentre cohort study was performed in German haematology/oncology centres. The incidence of probable and proven aspergillosis according to the revised EORTC/MSG criteria was assessed for all patients with acute leukaemia [acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL)]...
December 12, 2016: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27918843/cucurbita-ficifolia-cucurbitaceae-modulates-inflammatory-cytokines-and-ifn-%C3%AE-in-obese-mice
#9
Á Fortis-Barrera, R García-Macedo, J C Almanza-Perez, G Blancas-Flores, A Zamilpa-Alvarez, J L Flores-Sáenz, M Cruz, R Román-Ramos, F J Alarcón-Aguilar
This study investigated the effect of aqueous extract of Cucurbita ficifolia Bouché on systemic chronic inflammation in an obesity model induced by monosodium glutamate (MSG) via modulating the expression of adipokines (TNF-α, IL-6, resistin, and adiponectin) and immune-regulatory cytokines (IFN-γ and IL-10). Cucurbita ficifolia extract was administered daily by gavage to lean and MSG-obese mice for 30 days. At the end of treatment, cytokine mRNA expression in adipose tissue was determined by real-time polymerase chain reaction (PCR), and the protein levels of these cytokines were also quantified by enzyme-linked immunosorbent assay (ELISA)...
September 19, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/27890806/the-endocrine-disrupting-potential-of-monosodium-glutamate-msg-on-secretion-of-the-glucagon-like-peptide-1-glp-1-gut-hormone-and-glp-1-receptor-interaction
#10
Maeve Shannon, Brian Green, Gary Willars, Jodie Wilson, Natalie Matthews, Joanna Lamb, Anna Gillespie, Lisa Connolly
Monosodium glutamate (MSG) is a suspected obesogen with epidemiological evidence positively correlating consumption to increased body mass index and higher prevalence of metabolic syndrome. ELISA and high content analysis (HCA) were employed to examine the disruptive effects of MSG on the secretion of enteroendocrine hormone glucagon-like peptide-1 (GLP-1) and GLP-1 receptor (GLP-1R), respectively. Following 3h MSG exposure of the enteroendocrine pGIP/neo: STC-1 cell line model (500μg/ml) significantly increased GLP-1 secretion (1...
January 4, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/27880963/enhanced-production-of-gamma-aminobutyric-acid-by-optimizing-culture-conditions-of-lactobacillus-brevis-hye1-isolated-from-kimchi-a-korean-fermented-food
#11
Hee Seon Lim, In-Tae Cha, Seong Woon Roh, Hae-Hun Shin, Myung-Ji Seo
This study evaluated the effects of culture conditions, including carbon and nitrogen sources, L-monosodium glutamate (MSG), and initial pH, on gamma-aminobutyric acid (GABA) production by Lactobacillus brevis HYE1 isolated from kimchi, a Korean traditional fermented food. L. brevis HYE1 was screened by the production analysis of GABA and genetic analysis of glutamate decarboxylase gene, resulting in 14.64 mM GABA after 48 h of cultivation in MRS media containing 1% (w/v) MSG. In order to increase GABA production by L...
November 23, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27862370/comparative-evaluation-of-pan-fungal-real-time-pcr-galactomannan-and-1-3-%C3%AE-d-glucan-assay-for-invasive-fungal-infection-in-paediatric-cancer-patients
#12
Prashant Gupta, Abrar Ahmad, Vineeta Khare, Archana Kumar, Gopa Banerjee, Nitya Verma, Mastan Singh
Limited specific data and investigations are available for the diagnosis of Invasive Fungal Infection (IFI) in paediatrics cancer patients. Three non-invasive tests; Platelia Aspergillus EIA for galactomannan (GM), β-D-glucan (BDG) assay and pan-fungal real-time PCR for fungal DNA in blood were evaluated. One hundred twenty-five paediatrics cancer patients at the high risk of IFI were enrolled. Single blood and serum samples were evaluated by all the three methods. Patients were classified into 10 proven, 52 probable and 63 no IFI cases in accordance with EORTC MSG 2008 revised guidelines...
November 16, 2016: Mycoses
https://www.readbyqxmd.com/read/27837506/serological-approaches
#13
Barbora Weinbergerova, Iva Kocmanova, Zdenek Racil, Jiri Mayer
The diagnosis of invasive fungal diseases (IFD) based on clinical, radiological, and conventional microbiological findings is not reliable and is often delayed. Non-culture-based methods with higher sensitivity and specificity may reduce diagnostic time and result in decreased IFD morbidity and mortality. These methods are now increasingly used to manage patients at risk of IFD. Among available biomarkers, fungal antigens have been investigated as an aid to early diagnosis and are predominantly used as screening tests to prompt antifungal treatment mainly in patients with hematological malignancies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27832295/present-global-situation-of-amino-acids-in-industry
#14
Naoto Tonouchi, Hisao Ito
At present, amino acids are widely produced and utilized industrially. Initially, monosodium glutamate (MSG) was produced by extraction from a gluten hydrolysate. The amino acid industry started using the residual of the lysate. The discovery of the functions of amino acids has led to the expansion of their field of use. In addition to seasoning and other food use, amino acids are used in many fields such as animal nutrients, pharmaceuticals, and cosmetics. On the other hand, the invention of the glutamate fermentation process, followed by the development of fermentation methods for many other amino acids, is no less important...
November 11, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27825986/intracerebroventricular-injection-of-ghrelin-decreases-wheel-running-activity-in-rats
#15
Yumiko Miyatake, Tetsuya Shiuchi, Kazuaki Mawatari, Satomi Toda, Yasuko Taniguchi, Akari Futami, Fukiko Sato, Masashi Kuroda, Mayu Sebe, Rie Tsutsumi, Nagakatsu Harada, Yasuhiko Minokoshi, Tadahiro Kitamura, Koro Gotoh, Masaki Ueno, Yutaka Nakaya, Hiroshi Sakaue
There is an increasing interest in elucidating the molecular mechanisms by which voluntary exercise is regulated. In this study, we examined how the central nervous system regulates exercise. We used SPORTS rats, which were established in our laboratory as a highly voluntary murine exercise model. SPORTS rats showed lower levels of serum ghrelin compared with those of the parental line of Wistar rats. Intracerebroventricular and intraperitoneal injection of ghrelin decreased wheel-running activity in SPORTS rats...
January 2017: Peptides
https://www.readbyqxmd.com/read/27815226/utility-of-the-serum-galactomannan-assay-for-the-diagnosis-of-invasive-aspergillosis-in-children-with-acute-lymphoblastic-leukemia
#16
Gulhadiye Avcu, Deniz Yilmaz Karapinar, Ayse Burcu Akinci, Zuhal Onder Sivis, Akkiz Sahin, Zumrut Sahbudak Bal, Suleyha Hilmioglu Polat, Dilek Yesim Metin, Fadil Vardar, Yesim Aydinok
OBJECTIVES: Invasive aspergillosis (IA) is an important cause of mortality and morbidity in children with hematological malignancies. The monitoring of serum galactomannan (GM) antigen is considered useful in the diagnosis of IA . The aim of this study was to determine the utility of serum GM monitoring in the early diagnosis of IA and the role of positive antigenemia in the management of children with acute lymphoblastic leukemia (ALL). METHODS: The cases of 141 children who were being treated for ALL in the Division of Pediatric Hematology of the Medical School of Ege University between January 2006 and February 2015 were reviewed retrospectively...
January 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27795339/prospective-biomarker-screening-for-diagnosis-of-invasive-aspergillosis-in-high-risk-pediatric-patients
#17
Juergen Loeffler, Julia Hafner, Carlo Mengoli, Clemens Wirth, Claus Peter Heussel, Claudia Löffler, P Lewis White, Andrew J Ullmann, Denise Michel, Verena Wiegering, Matthias Wölfl, Paul Gerhardt Schlegel, Hermann Einsele, Jan Springer, Matthias Eyrich
Combined biomarker screening is increasingly used to diagnose invasive aspergillosis (IA) in high-risk patients. In adults, the combination of galactomannan (GM) and fungal DNA detection has proven to be beneficial in the diagnosis of IA. Data in purely pediatric cohorts are scarce. Here, we monitored 39 children shortly before and after allogeneic stem cell transplantation twice weekly by use of a commercial GM enzyme-linked immunosorbent assay (ELISA) and a PCR assay based on amplification of the pan-Aspergillus ITS1/5...
January 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27791369/formation-of-3-mcpd-fatty-acid-esters-from-monostearoyl-glycerol-and-the-thermal-stability-of-3-mcpd-monoesters
#18
Yue Zhao, Yaqiong Zhang, Zhongfei Zhang, Jie Liu, Yi-Lin Wang, Boyan Gao, Yuge Niu, Xiangjun Sun, Liangli Yu
Formation of 3-monochloropropanediol (3-MCPD) esters from monostearoyl glycerol (MSG) was investigated under high temperature and low moisture conditions. Different organic and inorganic chlorides, including lindane, KCl, CaCl2, NaCl, MgCl2, AlCl3, CuCl2, MnCl2, SnCl2, ZnCl2, and FeCl3, were evaluated for their potential to react with MSG to form 3-MCPD and glycidyl esters at 120 and 240 °C using a UPLC-Q-TOF MS analysis. The results indicated that different chlorine compounds differed in their capacity to react with MSG and formed different products including 3-MCPD mono- and diesters, distearoylglycerol, and glycidyl esters...
November 23, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27744310/galactomannan-testing-and-aspergillus-pcr-in-same-day-bronchoalveolar-lavage-and-blood-samples-for-diagnosis-of-invasive-aspergillosis
#19
Susanne Eigl, Martin Hoenigl, Birgit Spiess, Sven Heldt, Juergen Prattes, Peter Neumeister, Albert Wolfler, Jasmin Rabensteiner, Florian Prueller, Robert Krause, Mark Reinwald, Holger Flick, Dieter Buchheidt, Tobias Boch
In recent years galactomannan antigen testing (GM) and also Aspergillus PCR have become increasingly important for diagnosis of invasive aspergillosis (IA). Whether or not these tests need to be performed with bronchoalveolar lavage fluid (BALF; i.e., primary site of infection), or testing of blood samples is sufficient, remains, however, a matter of debate. We evaluated the diagnostic performance of GM ELISA, and Aspergillus PCR by using BALF samples and blood samples obtained at the same day from a total of 53 immunocompromised patients (16 with probable/proven IA and 37 with no evidence of IA according to the revised EORTC/MSG criteria; 38 patients with hematological malignancies were prospectively enrolled at the Medical University of Graz, Austria, 15 patients with mixed underlying diseases at the Mannheim University Hospital)...
October 14, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27725383/numerical-evaluation-of-efficacy-of-glutamate-on-gastrointestinal-motility-rapid-mri-study
#20
Shinsuke Nakayama, Hidemi Teramoto
 The umami taste amino acid, glutamate acts as a signaling molecule in multiple cellular systems in the body, including the brain and gastrointestinal tract. Therefore, glutamate may affect appetite by modulating gastrointestinal motility as well as through taste perception. In this study, we examined the effect of glutamate on gastric emptying and duodenal motility, by using rapid magnetic resonance imaging (MRI). Ten healthy male volunteers participated in the measurements. Abdominal coronal MR images were successively acquired after ingestion of a liquid meal with and without monosodium L-glutamate (MSG)...
2016: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
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