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https://www.readbyqxmd.com/read/28642144/performance-of-aspergilluspcr-in-cerebrospinal-fluid-for-the-diagnosis-of-cerebral-aspergillosis
#1
Sébastien Imbert, Jean-Yves Brossas, Martine Palous, Isabelle Joly, Isabelle Meyer, Arnaud Fekkar
OBJECTIVES: cerebral aspergillosis is a rare but often fatal form of invasive aspergillosis that remains difficult to diagnose. The literature has shown the value of Aspergillus PCR in blood-derived samples for the diagnosis of invasive aspergillosis but provides far less information for cerebrospinal fluid (CSF) in cerebral aspergillosis. Here, we evaluated the usefulness of an Aspergillus PCR assay performed on CSF for the diagnosis of cerebral aspergillosis. METHODS: this retrospective study involved 72 patients with suspected cerebral aspergillosis for a total of 88 CSF samples in whom CSF Aspergillus PCR was performed...
June 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28620676/retrospective-study-of-the-correlation-between-pathological-tumor-size-and-survival-after-curative-resection-of-t3-pancreatic-adenocarcinoma-proposal-for-reclassification-of-the-tumor-extending-beyond-the-pancreas-based-on-tumor-size
#2
Masanao Kurata, Goro Honda, Yoshiaki Murakami, Kenichiro Uemura, Sohei Satoi, Fuyuhiko Motoi, Masayuki Sho, Ippei Matsumoto, Manabu Kawai, Hiroaki Yanagimoto, Takumi Fukumoto, Minako Nagai, Masahiko Gosho, Michiaki Unno, Hiroki Yamaue
BACKGROUND: Even though most patients who undergo resection of pancreatic adenocarcinoma have T3 disease with extra-pancreatic tumor extension, T3 disease is not currently classified by tumor size. The aim of this study was to modify the current TNM classification of pancreatic adenocarcinoma to reflect the influence of tumor size. METHODS: A total of 847 consecutive pancreatectomy patients were recruited from multiple centers. Optimum tumor size cutoff values were calculated by receiver operating characteristics analysis for tumors limited to the pancreas (T1/2) and for T3 tumors...
June 15, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28620442/efficacy-of-nanoceria-for-periodontal-tissues-alteration-in-glutamate-induced-obese-rats-multidisciplinary-considerations-for-personalized-dentistry-and-prevention
#3
Tetyana V Beregova, Karine S Neporada, Maksym Skrypnyk, Tetyana M Falalyeyeva, Nadiya M Zholobak, Oleksandr B Shcherbakov, Mykola Ya Spivak, Rostyslav V Bubnov
BACKGROUND: Nowadays, we face the global epidemic of obesity, that is known to contribute to the development of many diseases, such as the oral cavity pathologies. Dental and oral pathologies are frequently caused by and overlapped with systemic multifactorial diseases such as obesity being its early indicators and risk factors. The aim was to study the influence of nanoceria on periodontal tissues alteration in glutamate (MSG)-induced obese rats. METHODS: We included 52 Wistar rats of both genders and divided into four groups: newborn rats in group 1 (control) received subcutaneously 8 μl/g saline...
March 2017: EPMA Journal
https://www.readbyqxmd.com/read/28618758/investigation-of-the-behavioral-and-neurochemical-effects-of-monosodium-glutamate-on-neonatal-rats
#4
Ayşen Çetin Kardeşler, Eyüp Başkale
BACKGROUND/AIM: The objective of this study was to investigate and analyze the behavioral and neurochemical effects of monosodium glutamate (MSG) injections at various and subsequent dosages on male Wistar rats during the neonatal period. MATERIALS AND METHODS: In order to determine the behavioral and neurochemical effects of MSG, the experiment was implemented on neonatal male Wistar rats and the test was repeated for various MSG dosages. After completing the experiment, additionally, levels of dopamine, GABA, catecholamine (dopamine, noradrenaline, and adrenaline) and glutamate in the brain cells of the decapitated rats were also measured using the ELISA method...
June 12, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28614713/acute-lesioning-and-rapid-repair-of-hypothalamic-neurons-outside-the-blood-brain-barrier
#5
Ernie Yulyaningsih, Ivan A Rudenko, Martin Valdearcos, Emma Dahlén, Eirini Vagena, Alvin Chan, Arturo Alvarez-Buylla, Christian Vaisse, Suneil K Koliwad, Allison W Xu
Neurons expressing agouti-related protein (AgRP) are essential for feeding. The majority of these neurons are located outside the blood-brain barrier (BBB), allowing them to directly sense circulating metabolic factors. Here, we show that, in adult mice, AgRP neurons outside the BBB (AgRP(OBBB)) were rapidly ablated by peripheral administration of monosodium glutamate (MSG), whereas AgRP neurons inside the BBB and most proopiomelanocortin (POMC) neurons were spared. MSG treatment induced proliferation of tanycytes, the putative hypothalamic neural progenitor cells, but the newly proliferated tanycytes did not become neurons...
June 13, 2017: Cell Reports
https://www.readbyqxmd.com/read/28612917/neonatal-exposure-to-monosodium-glutamate-results-in-dysmorphology-of-orofacial-lower-motor-neurons
#6
Lindsey Foran, Chloe Kupelian, Swati Laroia, Jeffrey Esper, Randy Joseph Kulesza
Glutamate is the most abundant excitatory neurotransmitter in the central nervous system, and is stored and released by both neurons and astrocytes. Despite the important role of glutamate as a neurotransmitter, high levels of extracellular glutamate can result in excitotoxicity and apoptosis. Monosodium glutamate (MSG) is a naturally occurring sodium salt of glutamic acid that is used as a flavor enhancer in many processed foods. Neonatal exposure to MSG has been shown to result in neurodegeneration in several forebrain regions, characterized by neuronal loss and neuroendocrine abnormalities...
June 14, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/28611499/lactobacillus-futsaii-cs3-a-new-gaba-producing-strain-isolated-from-thai-fermented-shrimp-kung-som
#7
Chatthaphisuth Sanchart, Onnicha Rattanaporn, Dietmar Haltrich, Pimpimol Phukpattaranont, Suppasil Maneerat
Kung-Som is a popular traditional Thai fermented shrimp product. It is rich in glutamic acid, which is the major substrate for the biosynthesis of gamma-aminobutyric acid (GABA) by lactic acid bacteria (LAB). In the present study, LAB from Kung-Som were isolated, screened for GABA formation, and the two isolates that transform glutamic acid most efficiently into GABA were identified. Based on the API-CHL50 fermentation profile and a phylogenetic tree of 16S rDNA sequences, strain CS3 and CS5 were identified as Lactobacillus futsaii, which was for the first time shown to be a promising GABA producer...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28579479/anti-obesogenic-and-hypolipidemic-effects-of-a-glucagon-like-peptide-1-receptor-agonist-derived-from-the-saliva-of-the-gila-monster
#8
Patricia Lucio Alves, Fernando Mauricio Francis Abdalla, Rafaela Fadoni Alponti, Paulo Flavio Silveira
INTRODUCTION: Glucagon-like peptide-1 (GLP-1) receptor (R) agonists are a class of incretin mimetic drugs that have been used for the treatment of type 2 diabetes mellitus and also considered strong candidates for the treatment of obesity. The original prototypical drug in this class is the exenatide, a synthetic peptide with the same structure as the native molecule, exendin-4, found in the saliva of the Gila monster (Heloderma suspectum suspectum lizard). OBJECTIVES: To identify and compare the anti-obesogenic, antidyslipidemic and antidiabetogenic effects of agonism in GLP-1R by exenatide on two distinct models of obesity: induced by hypothalamic injury (MSG) or high-calorie diet (DIO)...
June 1, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28528338/chronic-glibenclamide-treatment-attenuates-walker-256-tumour-growth-in-prediabetic-obese-rats
#9
Claudinéia Conationi da Silva Franco, Carina Previate, Kátia Gama de Barros Machado, Silvano Piovan, Rosiane Aparecida Miranda, Kelly Valério Prates, Veridiana Mota Moreira, Júlio Cezar de Oliveira, Luiz Felipe Barella, Rodrigo Mello Gomes, Flávio Andrade Francisco, Isabela Peixoto Martins, Audrei Pavanello, Tatiane Aparecida Ribeiro, Laize Peron Tófolo, Ananda Malta, Aline Amenencia de Souza, Vander Silva Alves, Sandra da Silva Silveira, Maria Raquel Marçal Natali, Jean Carlos Fernando Besson, Hely de Morais, Helenir Medri de Souza, Juliane Rocha de Sant Anna, Marialba Avezum Alves de Castro Prado, Paulo Cezar de Freitas Mathias
BACKGROUND/AIMS: The sulphonylurea glibenclamide (Gli) is widely used in the treatment of type 2 diabetes. In addition to its antidiabetic effects, low incidences of certain types of cancer have been observed in Gli-treated diabetic patients. However, the mechanisms underlying this observation remain unclear. The aim of the present work was to evaluate whether obese adult rats that were chronically treated with an antidiabetic drug, glibenclamide, exhibit resistance to rodent breast carcinoma growth...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28526877/magnetospinography-visualizes-electrophysiological-activity-in-the-cervical-spinal-cord
#10
Satoshi Sumiya, Shigenori Kawabata, Yuko Hoshino, Yoshiaki Adachi, Kensuke Sekihara, Shoji Tomizawa, Masaki Tomori, Senichi Ishii, Kyohei Sakaki, Dai Ukegawa, Shuta Ushio, Taishi Watanabe, Atsushi Okawa
Diagnosis of nervous system disease is greatly aided by functional assessments and imaging techniques that localize neural activity abnormalities. Electrophysiological methods are helpful but often insufficient to locate neural lesions precisely. One proposed noninvasive alternative is magnetoneurography (MNG); we have developed MNG of the spinal cord (magnetospinography, MSG). Using a 120-channel superconducting quantum interference device biomagnetometer system in a magnetically shielded room, cervical spinal cord evoked magnetic fields (SCEFs) were recorded after stimulation of the lower thoracic cord in healthy subjects and a patient with cervical spondylotic myelopathy and after median nerve stimulation in healthy subjects...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28522248/adverse-effects-in-kidney-function-antioxidant-systems-and-histopathology-in-rats-receiving-monosodium-glutamate-diet
#11
María Del Carmen Contini, Ana Fabro, Néstor Millen, Adriana Benmelej, Stella Mahieu
We investigated the effects of adding of monosodium glutamate (MSG) to a standard diet on oxidative stress in kidney, nitric oxide excretion, renal ions handling and blood pressure. We examined the association of these changes with the effects on renal histology. The study was performed on male Wistar rats (5 weeks old) divided into 3 groups: 1) MSG group were fed a diet supplemented with 3g of MSG/kg b.w./day, five days a week, and spontaneous ingestion of a 1% MSG solution during 16 weeks; 2) NaCl group were fed a diet with NaCl (1g/kg b...
May 15, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28515217/an-analytical-and-clinical-evaluation-of-the-pathonostics-aspergenius%C3%A2-assay-for-the-detection-of-invasive-aspergillosis-and-resistance-to-azole-antifungal-drugs-direct-from-plasma-samples
#12
P Lewis White, Raquel B Posso, Rosemary A Barnes
With the proposal to include Aspergillus PCR in the revised EORTC/MSG definitions for fungal disease, commercially manufactured assays may be required to provide standardisation and accessibility. The PathoNostics AsperGenius® assay represents one such test that has the ability to detect a range of Aspergillus species and azole-resistance in A. fumigatus Performance has been validated when testing BAL and serum specimens, but recent evidence suggests that testing plasma may enhance sensitivity over serum. It was decided to evaluate the analytical and clinical performance of the PathoNostics AsperGenius® assay when testing plasma...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28513268/effects-of-volume-training-on-strength-and-endurance-of-back-muscles-a-randomized-controlled-trial
#13
Leonardo Shigaki, Cynthia Gobbi Alves Araújo, Mariane Guizeline Calderon, Thais Karoline Cezar Costa, Andreo Fernando Aguiar, Leonardo Oliveira Pena Costa, Rubens A da Silva
CONTEXT: Strength/resistance training volume has historically been supported in the American College of Sports Medicine recommendations. However, for the back muscles, exercise prescription related to the number of sets, such as single vs. multiple, is not well established in the literature. OBJECTIVE: The purpose of this study was to compare the effects of two training volumes on strength and endurance of back extensor muscles in untrained young participants, with regard to a repeated measures design...
May 17, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28498192/patterns-of-attendance-at-mother-support-groups-in-zimbabwe-the-epaz-trial-2014-2016
#14
Joanna Orne-Gliemann, Hélène Font, Talent Maphosa, Abigail Kangwende, Simba Rusakaniko, Vhumani Magezi, Tonderai Sengai, Bridget Shumba, Pemberai Zambezi, Geoff Foster
BACKGROUND: In Southern Africa, groups of mothers living with HIV successfully provide peer psychosocial support during pregnancy and early childhood. We report patterns of attendance at Mother Support Groups (MSGs) sessions among women and mothers living with HIV in rural Zimbabwe. METHODS: In the Elimination of Pediatric AIDS cluster-randomized trial, MSGs were established in 14 health facilities in Mutare and Makoni districts. Patterns of attendance at MSG sessions were categorized by calendar attendance from delivery to 6-month postpartum using a Dynamic Time Warping clustering method...
June 1, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28498191/impact-of-facility-based-mother-support-groups-on-retention-in-care-and-pmtct-outcomes-in-rural-zimbabwe-the-epaz-cluster-randomized-controlled-trial
#15
Geoff Foster, Joanna Orne-Gliemann, Hélène Font, Abigail Kangwende, Vhumani Magezi, Tonderai Sengai, Simba Rusakaniko, Bridget Shumba, Pemberai Zambezi, Talent Maphosa
BACKGROUND: Prevention of mother-to-child transmission elimination goals are hampered by low rates of retention in care. The Eliminating Paediatric AIDS in Zimbabwe project assessed whether mother support groups (MSGs) improve rates of retention in care of HIV-exposed infants and their HIV-positive mothers, and maternal and infant outcomes. METHODS: The study involved 27 rural clinics in eastern Zimbabwe. MSGs were established in 14 randomly selected clinics and met every 2 weeks coordinated by volunteer HIV-positive mothers...
June 1, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28486943/kb-r7943-reduces-4-aminopyridine-induced-epileptiform-activity-in-adult-rats-after-neuronal-damage-induced-by-neonatal-monosodium-glutamate-treatment
#16
Mariana Hernandez-Ojeda, Monica E Ureña-Guerrero, Paola E Gutierrez-Barajas, Jazmin A Cardenas-Castillo, Antoni Camins, Carlos Beas-Zarate
BACKGROUND: Neonatal monosodium glutamate (MSG) treatment triggers excitotoxicity and induces a degenerative process that affects several brain regions in a way that could lead to epileptogenesis. Na(+)/Ca(2+) exchangers (NCX1-3) are implicated in Ca(2+) brain homeostasis; normally, they extrude Ca(2+) to control cell inflammation, but after damage and in epilepsy, they introduce Ca(2+) by acting in the reverse mode, amplifying the damage. Changes in NCX3 expression in the hippocampus have been reported immediately after neonatal MSG treatment...
May 9, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28470741/canine-oral-mucosa-evaluation-as-a-potential-autograft-tissue-for-the-treatment-of-unresponsive-keratoconjunctivitis-sicca
#17
Rose L Cherry, Jodi D Smith, Gil Ben-Shlomo
PURPOSE: Labial mucosa transplantation for the treatment of canine keratoconjunctivitis sicca (KCS) has been reported recently. Postoperative alleviation of clinical signs was noted and assumed to be the result of labial salivary glands providing lubrication to the ocular tissue. The aim of this study was to evaluate the presence of minor salivary glands (MSG) in the canine oral mucosa. METHODS: Oral mucosal biopsies were collected from six dogs that died (n = 1) or were euthanized (n = 5) for reasons unrelated to this study...
May 4, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28460669/pig-sperm-preincubation-and-gamete-coincubation-with-glutamate-enhance-sperm-oocyte-binding-and-in%C3%A2-vitro-fertilization
#18
M Spinaci, D Bucci, B Gadani, E Porcu, C Tamanini, G Galeati
As the taste receptor for monosodium glutamate (umami) is expressed in both murine and human spermatozoa and the presence of α-gustducin and α-transducin, G proteins involved in the umami taste signaling, has been described in boar germ cells, the aim of this study was to evaluate if monosodium glutamate (MSG) would exert any effect on sperm-oocyte binding, in vitro fertilization (IVF) and sperm parameters during in vitro induced capacitation. For sperm-zona pellucida binding assay, boar spermatozoa were preincubated for 1 h and then coincubated for 1 h with denuded in vitro matured oocytes in presence of different concentrations of MSG (0, 0...
June 2017: Theriogenology
https://www.readbyqxmd.com/read/28438014/characterization-of-a-glutamate-decarboxylase-gad-from-enterococcus-avium-m5-isolated-from-jeotgal-a-fermented-korean-sea-food
#19
Kang Wook Lee, Jae Min Shim, Zhuang Yao, Jeong A Kim, Hyun-Jin Kim, Jeong Hwan Kim
To develop starters for the production of functional foods or materials, lactic acid bacteria producing γ-aminobutyric acid (GABA) were screened from jeotgals, Korean fermented sea foods. One isolate producing high amount of GABA from monosodium L-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced 18.47 ± 1.26 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with MSG (3%, w/v). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E...
April 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28436061/resistance-exercise-reduces-memory-impairment-induced-by-monosodium-glutamate-in-male-and-female-rats
#20
Paulo Cesar Oliveira Araujo, Caroline Brandão Quines, Natália Silva Jardim, Marlon Regis Leite, Cristina Wayne Nogueira
What is the central question of this study? Monosodium glutamate causes cognitive impairment. Does resistance exercise improve the performance of rats treated with monosodium glutamate? What is the main finding and its importance? Resistance exercise is effective against monosodium glutamate-induced memory impairment in male and female rats. Monosodium glutamate (MSG), a flavour enhancer in diets, causes cognitive impairment in rodents. Exercise has been reported to protect against impairment of memory in humans...
April 23, 2017: Experimental Physiology
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