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https://www.readbyqxmd.com/read/29155566/structure-based-insights-into-dynamics-and-function-of-two-domain-slpa-from-escherichia-coli
#1
Anne-Juliane Geitner, Ulrich Weininger, Hauke Paulsen, Jochen Balbach, Michael Kovermann
SlpA (SlyD like protein A) comprises two domains, a FK506 binding domain (FKBP fold) of moderate prolyl cis/trans isomerase activity and an inserted in flap (IF) domain that hosts its chaperone activity. Here we present the NMR solution structure of apo E. coli SlpA determined by NMR which mirrors the structural properties seen for various SlyD homologs. Crucial structural differences in side-chain orientation arise for F37 which points directly into the hydrophobic core of the active site. It forms a prominent aromatic stacking with F15, one of the key residues for PPIase activity, thus giving a possible explanation for the inherently low PPIase activity of SlpA...
November 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/29154996/selective-binding-magnetic-separation-and-purification-of-histidine-tagged-protein-using-biopolymer-magnetic-core-shell-nanoparticles
#2
Yang Zhou, Dandan Yan, Shaofei Yuan, Yawen Chen, Emmanuella E Fletcher, Haifeng Shi, Bangxing Han
In previous studies, we synthesized the magnetic core-shell structured Fe3O4/PMG/IDA-Ni(2+) nanoparticles. The Ni(2+) on the surface of nanoparticles provides abundant docking sites for histidine, and the composite nanoparticles showed potential applications in the separation and purification of histidine-tagged (His-tagged) proteins. Meanwhile, the presence of the superparamagnetic core (Fe3O4) in the nanoparticles allows them to be quickly separated and purified by an external magnetic field. Herein, the ability of magnetic nanoparticles to purify His-tagged human superoxide dismutase 1 (hSOD1) was verified...
November 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29153949/a-novel-label-free-electrochemical-immunosensor-for-ultra-sensitively-detecting-prostate-specific-antigen-based-on-the-enhanced-catalytic-currents-of-oxygen-reduction-catalyzed-by-core-shell-au-pt-nanocrystals
#3
Rui Wang, Ai-Jun Wang, Wei-Dong Liu, Pei-Xin Yuan, Yadong Xue, Xiliang Luo, Jiu-Ju Feng
Herein, bimetallic core-shell Au@Pt nanocrystals (Au@Pt NCs) were prepared by a simple one-pot aqueous method using 2-pyrrolidone-5-carboxylic acid sodium salt (PCA-Na) as a new and green growth-directing agent. The obtained architectures displayed excellent catalytic activity towards oxygen reduction reaction (ORR) compared with commercial Pt/C catalyst. A novel immunosensor was constructed via assembly prostate specific antibody on the surface of Au@Pt NCs. It was found that the ORR currents were significantly suppressed due to the specific antigen-antibody reaction...
November 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29153652/enzymes-catalyzing-pre-hydrolysis-facilitated-the-anaerobic-fermentation-of-waste-activated-sludge-with-acidogenic-and-microbiological-perspectives
#4
Xiaodong Xin, Junguo He, Lin Li, Wei Qiu
This study investigated acidogenic and microbiological perspectives in the anaerobic fermentation (AF) of waste activated sludge (WAS) pre-hydrolyzed by enzymes catalysis. The enzymes catalysis boosted WAS biodegradability dramatically with nearly 8500 mg/L soluble chemical oxygen demand (SCOD) increase just within 4 h. The volatile fatty acids (VFAs) in the acidogenesis were accumulated effectively with over 3200 mg COD/L in 12 d, which reached 0.687 kWh/kg VSS electricity conversion efficiency (2...
October 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29152050/consequences-of-depsipeptide-substitution-on-the-clpp-activation-activity-of-antibacterial-acyldepsipeptides
#5
Yangxiong Li, Nathan P Lavey, Jesse A Coker, Jessica E Knobbe, Dat C Truong, Hongtao Yu, Yu-Shan Lin, Susan L Nimmo, Adam S Duerfeldt
The acyldepsipeptide (ADEP) antibiotics operate through a clinically unexploited mechanism of action and thus have attracted attention from several antibacterial development groups. The ADEP scaffold is synthetically tractable, and deep-seated modifications have produced extremely potent antibacterial leads against Gram-positive pathogens. Although newly identified ADEP analogs demonstrate remarkable antibacterial activity against bacterial isolates and in mouse models of bacterial infections, stability issues pertaining to the depsipeptide core remain...
November 9, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29151348/polysulfone-sio2-hybrid-shell-microcapsules-synthesized-by-the-combination-of-pickering-emulsification-and-solvent-evaporation-technique-and-their-application-in-self-lubricating-composites
#6
Haiyan Li, Shuang Li, Zhike Li, Yanji Zhu, Huaiyuan Wang
Lubricant oil-filled polysulfone/SiO2 (PSF/SiO2) hybrid shell microcapsules are prepared by the combination of Pickering emulsification and the solvent evaporation technique. Silica particles are used as stabilizers. The structure and properties of the microcapsules are influenced by the silica particle concentration, agitation speed, and encapsulation temperature. The formation of PSF/SiO2 hybrid microcapsules is confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and thermal gravimetric analysis (TGA)...
November 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29150150/biocompatible-hollow-polymeric-particles-produced-by-a-mild-solvent-and-template-free-strategy
#7
Eustolia Rodríguez-Velázquez, Pablo Taboada, Manuel Alatorre-Meda
Macroscopic hollow polymeric particles are attractive materials for various applications such as surgery, food industry, agriculture, etc. However, protocols reporting their synthesis have hitherto made use of organic solvents and/or sacrificial templates, compromising the encapsulation of different bioactive compounds and the process yield. Here, millimeter-size, hollow polymeric particles were synthesized, for the first time, in a solvent- and template free manner onto superhydrophobic surfaces (SHS). The particles were produced upon assembly and double superficial crosslinking of liquid droplets of DNA and methacrylamide chitosan aqueous solutions (CH:MA), leading to liquid-core particles with a hardened hydrogel shell...
November 14, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29149720/a-robust-strategy-for-preparation-of-sequential-stimuli-responsive-block-copolymer-prodrugs-via-thiolactone-chemistry-to-overcome-multiple-anticancer-drug-delivery-barriers
#8
Wendong Ke, Wei Yin, Zengshi Zha, Jean Felix Mukerabigwi, Weijian Chen, Yuheng Wang, Chuanxin He, Zhishen Ge
Block copolymer prodrugs (BCPs) have attracted considerable attentions in clinical translation of nanomedicine owing to their self-assembly into well-defined core-shell nanoparticles for improved pharmacokinetics, stability in blood circulation without drug leakage, and optimized biodistribution. However, a cascade of physiological barriers against specific delivery of drugs into tumor cells limit the final therapeutic efficacy. Herein, we report a robust and facile strategy based on thiolactone chemistry to fabricate well-defined BCPs with sequential tumor pH-promoted cellular internalization and intracellular stimuli-responsive drug release...
November 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/29149404/the-translational-controlled-tumour-protein-tctp-biological-functions-and-regulation
#9
Ulrich-Axel Bommer
The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein...
2017: Results and Problems in Cell Differentiation
https://www.readbyqxmd.com/read/29148796/layer-by-layer-self-assembly-of-polyelectrolytic-block-copolymer-worms-on-a-planar-substrate
#10
Nicholas Penfold, Andrew J Parnell, Marta Molina, Pierre Verstraete, Johan Smets, Steven P Armes
Cationic and anionic block copolymer worms are prepared by polymerization-induced self-assembly (PISA) via reversible addition-fragmentation chain transfer (RAFT) aqueous dispersion copolymerization of 2-hydroxypropyl methacrylate (HPMA) and glycidyl methacrylate (GlyMA), using a binary mixture of a non-ionic poly(ethylene oxide) (PEO) macromolecular RAFT agent and either a cationic poly([2-(methacryloyloxy)ethyl] trimethylammonium chloride) (PQDMA) or an anionic poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) macromolecular RAFT agent...
November 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29148708/magnetron-sputtering-deposition-cu-onion-like-n-c-as-high-performance-electrocatalysts-for-oxygen-reduction-reaction
#11
Hongyang Shao, Xiaoming Zhang, Hao Huang, Kan Zhang, Menglong Wang, Cai Zhang, Yifan Yang, Mao Wen, Weitao Zheng
The idea of a core-shell structure can promote the utilization of nonprecious metallic catalysts by enhancing their activity and stability for the oxygen reduction reaction (ORR). Developing a low-cost, high-efficiency, and high-reproducibility method for synthesizing core-shell-structured materials represents an urgent challenge. Here, we fabricate encapsulated Cu nanoparticles with nitrogen-doped onion-like graphite nanoshells (Cu@onion-like N-C) as an efficient ORR catalyst by magnetron sputtering, in which the graphite shells grow by an in situ self-assembly process activated by the surface-catalyzed behavior with Cu nanoparticles...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148695/a-high-thermal-and-air-stability-cathode-material-with-concentration-gradient-buffer-for-li-ion-batteries
#12
Ji-Lei Shi, Ran Qi, Xu-Dong Zhang, Peng-Fei Wang, Weigui Fu, Ya-Xia Yin, Jian Xu, Li-Jun Wan, Yu-Guo Guo
Delivery of high-capacity with high thermal and air stability is a great challenge in developing of Ni-rich layered cathodes for commercialized Li-ion batteries (LIBs). Herein we present a surface concentration-gradient spherical particle with varying elemental composition from the outer end LiNi1/3Co1/3Mn1/3O2 (NCM) to the inner end LiNi0.8Co0.15Al0.05O2 (NCA). This cathode material with the merit of NCM concentration-gradient protective buffer and the inner NCA core shows high capacity retention of 99.8% after 200 cycles at 0...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29147000/stable-icg-loaded-upconversion-nanoparticles-silica-core-shell-theranostic-nanoplatform-for-dual-modal-upconversion-and-photoacoustic-imaging-together-with-photothermal-therapy
#13
Ruichan Lv, Depeng Wang, Liyang Xiao, Guanying Chen, Jun Xia, Paras N Prasad
We report here the design and multiple functions of a new hierarchical nanotheronostic platform consisting of an upconversion nanoparticle (UCNP) core: shell with an additional mesoporous silica (mSiO2) matrix load shell containing sealed, high concentration of ICG molecules. We demonstrate that this UCNP@mSiO2-ICG nanoplatform can perform the following multiple functions under NIR excitation at 800 nm: 1) Light harvesting by the UCNP shell containing Nd and subsequent energy transfer to Er in the Core to produce efficient green and red upconversion luminescence for optical imaging; 2) Efficient nonradiative relaxation and local heating produced by concentration quenching in aggregated ICG imbedded in the mesopourous silica shell to enable both photoacoustic imaging and photothermal therapy...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146983/precise-control-of-alloying-sites-of-bimetallic-nanoclusters-via-surface-motif-exchange-reaction
#14
Qiaofeng Yao, Yan Feng, Victor Fung, Yong Yu, De-En Jiang, Jun Yang, Jianping Xie
Precise control of alloying sites has long been a challenging pursuit, yet little has been achieved for the atomic-level manipulation of metallic nanomaterials. Here we describe utilization of a surface motif exchange (SME) reaction to selectively replace the surface motifs of parent [Ag44(SR)30](4-) (SR = thiolate) nanoparticles (NPs), leading to bimetallic NPs with well-defined molecular formula and atomically-controlled alloying sites in protecting shell. A systematic mass (and tandem mass) spectrometry analysis suggests that the SME reaction is an atomically precise displacement of SR-Ag(I)-SR-protecting modules of Ag NPs by the incoming SR-Au(I)-SR modules, giving rise to a core-shell [Ag32@Au12(SR)30](4-)...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29145077/stability-of-the-diagnosis-of-seasonal-affective-disorder-in-a-long-term-prospective-study
#15
Marie-Laure Cléry-Melin, Philip Gorwood, Serge Friedman, Christian Even
BACKGROUND: Seasonal affective disorder (SAD) is mainly characterized by a seasonal pattern of depressive recurrences over the years. However, few studies have been conducted on the long-term course of patients with SAD, whose findings raised questions about the diagnosis stability over time. This study aimed to better characterize the diagnosis evolution, and determine prognosis markers. METHODS: An initial cohort of 225 outpatients diagnosed as having a SAD, was assessed at baseline (T1) for clinical symptoms and response to bright light therapy...
November 7, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29144757/versatile-encapsulation-technology-based-on-tailored-ph-responsive-amphiphilic-polymers-emulsion-gels-and-capsules
#16
Clémentine Locatelli-Champagne, Jean-Marc Suau, Olivier Guerret, Charlotte Pellet, Michel Cloitre
We present a multipurpose technology to encapsulate hydrophobic substances in micron-size emulsion droplets and capsules. The encapsulating agent is a comb-like stimuli-responsive copolymer comprising side chain surfactants attached to a methacrylic acid/ethylacrylate polyelectrolyte backbone. The composition and structure of the hydrophobic moieties of the side chains are customized in order to tune the particle morphology and the processing conditions. The technology exploits the synergy of properties provided by the copolymer: interfacial activity, pH responsiveness, and viscoelasticity...
November 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29144110/effect-of-stabilization-exercise-on-back-pain-disability-and-quality-of-life-in-adult-with-scoliosis-a-systematic-review
#17
Malik H Alanazi, Eric C Parent, Elizabeth Dennett
INTRODUCTION: Adult Scoliosis (AS) is the most common spine deformity in adults. Back pain is the main symptom leading patients to seek medical consultation. Stabilization exercise has been shown effective for reducing back pain. No literature review has examined the effects of such exercises in adults with scoliosis. OBJECTIVES: To systematically review the effects of stabilization exercises on back pain, disability and quality of life in adults with scoliosis...
November 16, 2017: European Journal of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/29141403/lipogels-for-encapsulation-of-hydrophilic-proteins-and-hydrophobic-small-molecules
#18
Celia C Homyak, Ann Fernandez, Mollie A Touve, Bo Zhao, Francesca Anson, Jeanne A Hardy, Richard W Vachet, Nathan C Gianneschi, Jennifer L Ross, S Thayumanavan
Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a one-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently-labeled lipids, protein, and hydrophobic molecules, we have characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV-Visible spectroscopy, fluorescence spectroscopy, and single particle total internal reflection fluorescence (TIRF) microscopy...
November 15, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29140685/heterogeneous-ti3sic2-c-containing-na2ti7o15-architecture-for-high-performance-sodium-storage-at-elevated-temperatures
#19
Guodong Zou, Qingrui Zhang, Carlos Fernandez, Gang Huang, Jianyu Huang, Qiuming Peng
Rational design of heterogeneous electrode materials with hierarchical architecture is a potential approach to significantly improve their energy densities. Herein, we report a tailored microwave-assisted synthetic strategy to create heterogeneous hierarchical Ti3SiC2@C-containing Na2Ti7O15 (MAX@C-NTO) composites as potential anode materials for high-performance sodium storage in a wide temperature range from 25 to 80 °C. This composite delivers first reversible capacities of 230 mAh g(-1) at 200 mA g(-1) and 149 mAh g(-1) at 3000 mA g(-1) at 25 °C...
November 17, 2017: ACS Nano
https://www.readbyqxmd.com/read/29140350/performance-optimization-of-hollow-core-fiber-photothermal-gas-sensors
#20
Yuechuan Lin, Wei Jin, Fan Yang, Yanzhen Tan, Hoi Lut Ho
We report performance optimization of hollow-core photonic bandgap fiber (HC-PBF) photothermal (PT) gas sensors. The PT phase modulation efficiency of a C2H2 filled HC-PBF (HC-1550-02) is found independent of the pump modulation frequency for up to ∼330  kHz, but starts to drop quickly to 10% of the maximum value at a couple of megahertz. With a 1.1 m long HC-PBF gas cell with angle-cleaved single-mode fiber/HC-PBF joints to reduce reflection and a modified 3×3 Sagnac interferometer with balanced detection for phase demodulation, a noise equivalent concentration of ∼67  ppbC2H2 is achieved with a 1 s time constant, and it goes down to ∼18  ppb with 145 s integration time...
November 15, 2017: Optics Letters
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