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https://www.readbyqxmd.com/read/28917755/identification-of-trypanosomatids-by-detecting-single-nucleotide-fingerprints-using-dna-analysis-by-dynamic-chemistry-with-maldi-tof
#1
María Angélica Luque-González, Mavys Tabraue-Chávez, Bárbara López-Longarela, Rosario María Sánchez-Martín, Matilde Ortiz-González, Miguel Soriano-Rodríguez, José Antonio García-Salcedo, Salvatore Pernagallo, Juan José Díaz-Mochón
Protozoan parasites of the Trypanosomatidae family can cause devastating diseases in humans and animals, such as Human African Trypanosomiasis or Sleeping Sickness, Chagas disease and Leishmaniasis. Currently, there are molecular assays for detecting parasitic infections and their post-treatment monitoring based on nucleic acid amplification, but there are still certain limitations which limit the development of assays that can detect and discriminate between parasite infections with a single test. Here, we present the development of a novel molecular assay for the rapid identification of Trypanosomatids, integrating DNA analysis by dynamic chemistry in conjunction with Matrix-Assisted Laser Desorption Ionization - Time-of-Flight Mass Spectrometry (MALDI-ToF)...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28913871/experimental-asbestos-studies-in-the-uk-1912-1950
#2
Morris Greenberg
The asbestos industry originated in the UK in the 1870s. By 1898, asbestos had many applications and was reported to be one of the four leading causes of severe occupational disease. In 1912, the UK government sponsored an experimental study that reported that exposure to asbestos produced no more than a modicum of pulmonary fibrosis in guinea pigs. In the 1930s, the newly established Medical Research Council, with assistance from industry, sponsored a study of the effects of exposing animals to asbestos by injection (intratracheal and subcutaneous) and by inhalation in the factory environment...
September 14, 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/28913764/drivers-challenges-and-opportunities-of-forage-technology-adoption-by-smallholder-cattle-households-in-cambodia
#3
K Ashley, S Wilson, J R Young, H P Chan, S Vitou, S Suon, P A Windsor, R D Bush
Forage technology has been successfully introduced into smallholder cattle systems in Cambodia as an alternative feed source to the traditional rice straw and native pastures, improving animal nutrition and reducing labour requirements of feeding cattle. Previous research has highlighted the positive impacts of forage technology including improved growth rates of cattle and household time savings. However, further research is required to understand the drivers, challenges and opportunities of forage technology for smallholder cattle households in Cambodia to facilitate widespread adoption and identify areas for further improvement...
September 14, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28912890/glioma-dual-targeting-nanohybrid-protein-toxin-constructed-by-intein-mediated-site-specific-ligation-for-multistage-booster-delivery
#4
Yingzhi Chen, Meng Zhang, Hongyue Jin, Dongdong Li, Fan Xu, Aihua Wu, Jinyu Wang, Yongzhuo Huang
Malignant glioma is one of the most untreatable cancers because of the formidable blood-brain barrier (BBB), through which few therapeutics can penetrate and reach the tumors. Biologics have been booming in cancer therapy in the past two decades, but their application in brain tumor has long been ignored due to the impermeable nature of BBB against effective delivery of biologics. Indeed, it is a long unsolved problem for brain delivery of macromolecular drugs, which becomes the Holy Grail in medical and pharmaceutical sciences...
2017: Theranostics
https://www.readbyqxmd.com/read/28911188/diversity-of-ochrobactrum-species-in-food-animals-antibiotic-resistance-phenotypes-and-polymorphisms-in-the-blaoch-gene
#5
Carla Andrea Alonso, Yakubu A Kwabugge, Madubuike U Anyanwu, Carmen Torres, Kennedy F Chah
Twenty-six lactose non-fermenting, oxidase, urease and citrate-positive Gram-negative rods, isolated from broiler chickens, pigs and cattle at slaughter, were subjected to the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rDNA sequencing for identification. Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Ochrobactrum isolates resistant to third-generation cephalosporins were PCR-screened for the presence of the Ochrobactrum anthropi ampC gene (blaOCH)...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28904061/a-randomized-controlled-trial-of-green-tea-extract-supplementation-and-mammographic-density-in-postmenopausal-women-at-increased-risk-of-breast-cancer
#6
Hamed Samavat, Giske Ursin, Tim H Emory, Eunjung Lee, Renwei Wang, Carolyn J Torkelson, Allison M Dostal, Karen Swenson, Chap T Le, Chung S Yang, Mimi C Yu, Douglas Yee, Anna H Wu, Jian-Min Yuan, Mindy S Kurzer
Epidemiological and animal studies suggest a protective role of green tea against breast cancer. However the underlying mechanism is not understood. We conducted a randomized, double-blinded, placebo-controlled phase II clinical trial to investigate whether supplementation with green tea extract (GTE) modifies mammographic density (MD), as a potential mechanism, involving 1,075 healthy postmenopausal women. Women assigned to the treatment arm consumed daily 4 decaffeinated GTE capsules containing 1315 mg total catechins including 843 mg epigallocatechin-3-gallate (EGCG) for 12 months...
September 13, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28903168/-sharing-bacterial-microbiota-between-owners-and-their-pets-dogs-cats
#7
Jan Wipler, Zuzana Čermáková, Tomáš Hanzálek, Hana Horáková, Helena Žemličková
INTRODUCTION: The microbiological aspect of a relationship between pets (dogs/cats) and their owners is mainly concerned with the incidence of shared bacterial species, in particular potential pathogens. Given the great popularity of sharing homes with pets (dogs/cats) in the Czech Republic, there is an increased possibility of communication between microbiota of the two macroorganisms (pet and owner). The aim of the study was to determine the biodiversity of shared bacteria and possibility of exchange of genes of resistance to antimicrobial agents between potential pathogens based on the close relationship between pets and humans...
June 2017: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28902267/-public-production-as-a-key-factor-for-access-to-antivenoms-in-the-region-of-the-americas
#8
Guillermo Temprano, Patricia Aprea, José Christian Dokmetjian
Injuries caused by venomous animals affect vast areas of Latin America, Southern Asia, Southeast Asia, sub-Saharan Africa, and Oceania, and pose a serious problem for global public health. Based on an analysis of the current panorama of global production of ophidian and arachnid antivenoms, it is concluded that they are semi-orphaned products. This is a favorable scenario in which to strengthen public laboratory production. Governments should make a political decision in this regard in the interest of equity in population health...
August 21, 2017: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28902173/concept-for-recycling-waste-biomass-from-the-sugar-industry-for-chemical-and-biotechnological-purposes
#9
Magdalena Modelska, Joanna Berlowska, Dorota Kregiel, Weronika Cieciura, Hubert Antolak, Jolanta Tomaszewska, Michał Binczarski, Elzbieta Szubiakiewicz, Izabela A Witonska
The objective of this study was to develop a method for the thermally-assisted acidic hydrolysis of waste biomass from the sugar industry (sugar beet pulp and leaves) for chemical and biotechnological purposes. The distillates, containing furfural, can be catalytically reduced directly into furfurayl alcohol or tetrahydrofurfuryl alcohol. The sugars present in the hydrolysates can be converted by lactic bacteria into lactic acid, which, by catalytic reduction, leads to propylene glycol. The sugars may also be utilized by microorganisms in the process of cell proliferation, and the biomass obtained used as a protein supplement in animal feed...
September 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28894896/-hypospadias-insights-and-challenges
#10
I Rübben, R Stein
Disorders of the ventral tubularization of the urethra, such as the hypospadias, are among the second most frequent congenital childhood malformations. An increasing incidence has been observed suggesting a doubling in the US, which could not be documented for the European area. The underlying causes of this congenital defect remain unidentified. Genetic risk constellations or environmental influences, in particular by so-called endocrine disrupting chemicals (EDCs), are discussed as triggering factors. Boys after in vitro fertilization are more likely to have hypospadias than in nonreproductive-assisted pregnancies...
September 11, 2017: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/28893081/paired-box-7-pax7-gene-molecular-characterization-polymorphism-and-its-association-with-growth-performance-in-goose-anser-cygnoides
#11
C Wang, Y Yang, Liu Yi, S Gong, Wang Ying, D He
1. Paired box (Pax7) gene is a member of the paired box family and plays a critical role in animal growth and muscle development. However, the molecular characterisation of the goose Pax7 gene is unknown. 2. The open reading frame of goose Pax7 is composed of 1509 bp, which encodes a protein of 503 amino acids and shares high homology with Pax7 of other birds. 3. Ten single nucleotide polymorphisms were identified in the genomic DNA sequence, 8 located in the intron region and two located in the exon region...
September 12, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28888516/effects-of-animal-assisted-activities-on-biobehavioral-stress-responses-in-hospitalized-children-a-randomized-controlled-study
#12
Sandra M Branson, Lisa Boss, Nikhil S Padhye, Thea Trötscher, Alexandra Ward
PURPOSE: This study assessed the effectiveness of animal-assisted activities (AAA) on biobehavioral stress responses (anxiety, positive and negative affect, and salivary cortisol and C-reactive protein [CRP] levels) in hospitalized children. DESIGN AND METHODS: This was a randomized, controlled study. METHOD: Forty-eight participants were randomly assigned to receive a 10-minute AAA (n=24) or a control condition (n=24). Anxiety, positive and negative affect, and levels of salivary biomarkers were assessed before and after the intervention...
September 2017: Journal of Pediatric Nursing
https://www.readbyqxmd.com/read/28888334/preparation-characterization-and-application-of-long-acting-fsh-analogs-for-assisted-reproduction
#13
David Ben-Menahem
Assisted reproduction technologies are widely used in humans and domestic animals and often include follicle stimulating hormone (FSH) in the protocol. One limitation with most of the available FSH preparations is the relative short half-life in the circulation that dictates multiple daily injections for the desired follicle development and superovulation. The development of bioactive long-acting structurally modified FSH analogs is desirable for human and veterinary use. In addition, optimal preparations and/or formulations are expected to improve the regimen and efficiency of the treatment...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28887718/measurement-of-hemodynamic-changes-with-the-axial-flow-blood-pump-installed-in-descending-aorta
#14
Eiji Okamoto, Tetsuya Yano, Hidekazu Miura, Yasuyuki Shiraishi, Tomoyuki Yambe, Yoshinori Mitamura
We have developed various axial flow blood pumps to realize the concept of the Valvo pump, and we have studied hemodynamic changes under cardiac assistance using an axial flow blood pump in series with the natural heart. In this study, we measured hemodynamic changes of not only systemic circulation but also cerebral circulation and coronary circulation under cardiac support using our latest axial flow blood pump placed in the descending aorta in an acute animal experiment. The axial flow blood pump was installed at the thoracic descending aorta through a left thoracotomy of a goat (43...
September 8, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/28882255/improvement-of-the-performance-of-animal-crossing-warning-signs
#15
Majid Khalilikhah, Kevin Heaslip
INTRODUCTION: Animal-vehicle collisions (AVCs) can result in serious injury and death to drivers, animals' death, and significant economic costs. However, the cost effectiveness of the majority of AVC mitigation measures is a significant issue. METHOD: A mobile-based data collection effort was deployed to measure signs under the Utah Department of Transportation's (UDOT) jurisdiction. The crash data were obtained from the UDOT risk management database. ArcGIS was employed to link these two data sets and extract animal-related crashes and signs...
September 2017: Journal of Safety Research
https://www.readbyqxmd.com/read/28881230/investigation-of-the-5-flanking-region-and-exon-3-polymorphisms-of-igf-1-gene-showed-moderate-association-with-semen-quality-in-sanjabi-breed-rams
#16
R Bakhtiar, A Abdolmohammadi, H Hajarian, Z Nikousefat, D Kalantar-Neyestanaki
In this study, semen samples were collected from 96 Sanjabi rams in order to investigate the IGF-1 gene polymorphisms and their relationship with the characteristics of semen quality and testicular size. The dimensions of scrotal length, width and circumference were measured during autumn and spring over two years. Blood samples were simultaneously collected from jugular vein to extract DNA. PCR was performed using specific primers to amplify 294 and 272bp fragments including 5' regulatory region and exon 3 of IGF-1 gene, respectively...
August 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28880161/unsupervised-segmentation-of-5d-hyperpolarized-carbon-13-mri-data-using-a-fuzzy-markov-random-field-model
#17
Ferdia A Gallagher, Charlie J Daniels
Hyperpolarized MRI with ¹³C-labelled compounds is an emerging clinical technique allowing in vivo metabolic processes to be characterized non-invasively. Accurate quantification of ¹³C data, both for clinical and research purposes, typically relies on the use of region-of-interest analysis to detect and compare regions of altered metabolism. However, it is not clear how this should be determined from the five-dimensional data produced and most standard methodologies are unable to exploit the multidimensional nature of the data...
September 4, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28878713/human-animal-interaction-and-older-adults-an-overview
#18
REVIEW
Nancy R Gee, Megan K Mueller, Angela L Curl
Both pet ownership and animal-assisted therapy are becoming increasingly popular in the United States, and the science of human-animal interaction (HAI) seeks to explore how these relationships with animals can impact health and well-being. In particular, one burgeoning area of research is the role of HAI in healthy aging, given the potential for HAI as an important feature of health and well-being in older adults. The purpose of this review is to summarize and evaluate existing research in this innovative area of scholarship, identifying the potential benefits and risks of both pet ownership and animals in therapeutic settings for older adults...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28878477/near-infrared-spectroscopy-can-reveal-increases-in-brain-activity-related-to-animal-assisted-therapy
#19
Yuka Morita, Fumio Ebara, Yoshimitsu Morita, Etsuo Horikawa
[Purpose] Previous studies have indicated that animal-assisted therapy can promote recovery of psychological, social, and physiological function in mental disorders. This study was designed as a pilot evaluation of the use of near-infrared spectroscopy to objectively identify changes in brain activity that could mediate the effect of animal-assisted therapy. [Subjects and Methods] The participants were 20 healthy students (10 males and 10 females; age 19-21 years) of the Faculty of Agriculture, Saga University...
August 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28874705/serum-lysophospholipid-levels-are-altered-in-dyslipidemic-hamsters
#20
Susana Suárez-García, Antoni Caimari, Josep Maria Del Bas, Manuel Suárez, Lluís Arola
Dyslipidemias are common disorders that predispose individuals to severe diseases. It is known that healthy living habits can prevent dyslipidemias if they are diagnosed properly. Therefore, biomarkers that assist in diagnosis are essential. The aim of this study was to identify biomarkers of dyslipidemia progression, which in turn disclose its etiology. These findings will pave the way for examinations of the regulatory mechanisms involved in dyslipidemias. Hamsters were fed either a normal-fat diet (NFD) or a high-fat diet...
September 5, 2017: Scientific Reports
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