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https://www.readbyqxmd.com/read/27932184/intrapancreatic-splenule-in-a-pancreas-allograft-case-report
#1
K Yadav, O K Serrano, R Kandaswamy
A 16-year-old white man was involved in a motor vehicle collision and suffered head, chest, and abdominal trauma. Despite initial resuscitative efforts, he progressed to brain death and was designated to be an organ donor by his family. He had no earlier medical or surgical history and no high-risk behaviors. Blood work revealed normal creatinine, liver function tests, lipase, and amylase. Viral serologies were negative except for cytomegalovirus IgG and Epstein-Barr virus nucleic acid. Imaging revealed a right kidney contusion, a manubrial fracture, and fractures of right first rib and bilateral scapulae...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27929402/extraction-and-quantification-of-bioactive-tyrian-purple-precursors-a-comparative-and-validation-study-from-the-hypobranchial-gland-of-a-muricid-dicathais-orbita
#2
Roselyn Valles-Regino, Peter Mouatt, David Rudd, Lachlan H Yee, Kirsten Benkendorff
Muricidae are marine molluscs known for the production of Tyrian purple and bioactive precursor compounds. A validation study for the extraction and analysis of secondary metabolites found in the hypobranchial gland of the muricid Dicathais orbita is reported, using high performance liquid chromatography-mass spectrometry (HPLC-MS) with diode array detector (DAD). Quantification of the dominant secondary metabolites from D. orbita is described, followed by a comparison of solvent extraction procedures and stability studies...
December 5, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27928782/a-method-for-decomposition-of-the-basic-reaction-of-biological-macromolecules-into-exponential-components
#3
Yu M Barabash, A K Lyamets
The structural and dynamical properties of biological macromolecules under non-equilibrium conditions determine the kinetics of their basic reaction to external stimuli. This kinetics is multiexponential in nature. This is due to the operation of various subsystems in the structure of macromolecules, as well as the effect of the basic reaction on the structure of macromolecules. The situation can be interpreted as a manifestation of the stationary states of macromolecules, which are represented by monoexponential components of the basic reaction (Monod-Wyman-Changeux model) Monod et al...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27928494/synthetically-tuneable-biomimetic-artificial-photosynthetic-reaction-centres-that-closely-resemble-the-natural-system-in-purple-bacteria
#4
Sai-Ho Lee, Iain M Blake, Allan G Larsen, James A McDonald, Kei Ohkubo, Shunichi Fukuzumi, Jeffrey R Reimers, Maxwell J Crossley
Porphyrin-based photosynthetic reaction centre (PRC) mimics, ZnPQ-Q2HP-C60 and MP2Q-Q2HP-C60 (M = Zn or 2H), designed to have a similar special-pair electron donor and similar charge-separation distances, redox processes and photochemical reaction rates to those in the natural PRC from purple bacteria, have been synthesised and extensive photochemical studies performed. Mechanisms of electron-transfer reactions are fully investigated using femtosecond and nanosecond transient absorption spectroscopy. In benzonitrile, all models show picosecond-timescale charge-separations and the final singlet charge-separations with the microsecond-timescale...
October 19, 2016: Chemical Science
https://www.readbyqxmd.com/read/27924127/ssr-based-genetic-diversity-of-pigmented-and-aromatic-rice-oryza-sativa-l-genotypes-of-the-western-himalayan-region-of-india
#5
Humaira Ashraf, Amjad M Husaini, M Ashraf Bhat, G A Parray, Salim Khan, Nazir A Ganai
A set of 24 of SSR markers were used to estimate the genetic diversity in 16 rice genotypes found in Western Himalayas of Kashmir and Himachal Pradesh, India. The level of polymorphism among the genotypes of rice was evaluated from the number of alleles and PIC value for each of the 24 SSR loci. A total of 68 alleles were detected across the 16 genotypes through the use of these 24 SSR markers The number of alleles per locus generated varied from 2 (RM 338, RM 452, RM 171) to 6 (RM 585, RM 249, RM 481, RM 162)...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27917182/expression-of-flavone-synthase-ii-and-flavonoid-3-hydroxylase-is-associated-with-color-variation-in-tan-colored-injured-leaves-of-sorghum
#6
Hiroshi Mizuno, Takayuki Yazawa, Shigemitsu Kasuga, Yuji Sawada, Hiroyuki Kanamori, Yuko Ogo, Masami Yokota Hirai, Takashi Matsumoto, Hiroyuki Kawahigashi
Sorghum (Sorghum bicolor L. Moench) exhibits various color changes in injured leaves in response to cutting stress. Here, we aimed to identify key genes for the light brown and dark brown color variations in tan-colored injured leaves of sorghum. For this purpose, sorghum M36001 (light brown injured leaves), Nakei-MS3B (purple), and a progeny, #7 (dark brown), from Nakei-MS3B × M36001, were used. Accumulated pigments were detected by using high-performance liquid chromatography: M36001 accumulated only apigenin in its light brown leaves; #7 accumulated both luteolin and a small amount of apigenin in its dark brown leaves, and Nakei-MS3B accumulated 3-deoxyanthocyanidins (apigeninidin and luteolinidin) in its purple leaves...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27909749/diversity-of-total-bacterial-communities-and-chemoautotrophic-populations-in-sulfur-rich-sediments-of-shallow-water-hydrothermal-vents-off-kueishan-island-taiwan
#7
Li Wang, Man Kit Cheung, Rulong Liu, Chong Kim Wong, Hoi Shan Kwan, Jiang-Shiou Hwang
Shallow-water hydrothermal vents (HTVs) are an ecologically important habitat with a geographic origin similar to that of deep-sea HTVs. Studies on shallow-water HTVs have not only facilitated understanding of the influences of vents on local ecosystems but also helped to extend the knowledge on deep-sea vents. In this study, the diversity of bacterial communities in the sediments of shallow-water HTVs off Kueishan Island, Taiwan, was investigated by examining the 16S ribosomal RNA gene as well as key functional genes involved in chemoautotrophic carbon fixation (aclB, cbbL and cbbM)...
December 1, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27904458/identification-of-novel-source-of-resistance-and-differential-response-of-allium-genotypes-to-purple-blotch-pathogen-alternaria-porri-ellis-ciferri
#8
Satyabrata Nanda, Subodh Kumar Chand, Purander Mandal, Pradyumna Tripathy, Raj Kumar Joshi
Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession 'CBT-Ac77' and cultivar 'Arka Kalyan' were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A...
December 2016: Plant Pathology Journal
https://www.readbyqxmd.com/read/27904399/identification-of-alleles-of-puroindoline-genes-and-their-effect-on-wheat-triticum-aestivum-l-grain-texture
#9
Mária Presinszká, Klára Štiasna, Tomáš Vyhnánek, Václav Trojan, Eva Mrkvicová, Luděk Hřivna, Ladislav Havel
Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivum L.). It is a significant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (Pina and Pinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity within Pina and Pinb (alleles: Pina-D1a, Pina-D1b, Pinb-D1a, Pinb- -D1b, Pinb-D1c and Pinb-D1d)...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27902845/cloning-and-heterologous-expression-of-chlorophyll-a-synthase-in-rhodobacter-sphaeroides
#10
Emre M Ipekoğlu, Koray Göçmen, Mehmet T Öz, Muazzez Gürgan, Meral Yücel
Rhodobacter sphaeroides is a purple non-sulfur bacterium which photoheterotrophically produces hydrogen from organic acids under anaerobic conditions. A gene coding for putative chlorophyll a synthase (chlG) from cyanobacterium Prochlorococcus marinus was amplified by nested polymerase chain reaction and cloned into an inducible-expression plasmid which was subsequently transferred to R. sphaeroides for heterologous expression. Induced expression of chlG in R. sphaeroides led to changes in light absorption spectrum within 400-700 nm...
November 30, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27902294/actinocrinis-puniceicyclus-gen-nov-sp-nov-a-novel-actinobacterium-isolated-from-an-acidic-spring-in-kootenay-national-park-canada
#11
Joong-Jae Kim, Colbran E Marjerrison, Sabrina L Cornish Shartau, Allyson L Brady, Christine E Sharp, W Irene C Rijpstra, Jaap S Sinninghe Damsté, Peter Schumann, Stephen E Grasby, Peter F Dunfield
An aerobic, mildly acidophilic actinobacterium was isolated from the Ochre Beds bog in Kootenay National Park, Canada. Cells of isolate OB1T were Gram-positive, non-motile, pink-to purple-pigmented filaments. The pH range for growth was 3.5 - 6.5 (optimum 5.5), and the temperature range was 13-37 oC. The major cellular fatty acids were 16:0 iso (28.5 %), 15:0 iso (14.6 %) and 15:0 anteiso (14.3 %), and the major polar lipid was phosphohexose. The major quinone was menaquinone-11 (MK-11) and the peptidoglycan type was A1γ...
November 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27896813/transcriptional-and-post-transcriptional-regulation-of-histone-variant-h2a-z-during-sea-urchin-development
#12
Mihai Hajdu, Jasmine Calle, Andrea Puno, Aminat Haruna, César Arenas-Mena
Histone variant H2A.Z promotes chromatin accessibility at transcriptional regulatory elements and is developmentally regulated in metazoans. We characterize the transcriptional and post-transcriptional regulation of H2A.Z in the purple sea urchin Strongylocentrotus purpuratus. H2A.Z depletion by antisense translation-blocking morpholino oligonucleotides during early development causes developmental collapse, in agreement with its previously demonstrated general role in transcriptional multipotency. During H2A...
November 29, 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27887737/polyphenolic-diversity-and-characterization-in-the-red-purple-berries-of-east-asian-wild-vitis-species
#13
Kazuya Koyama, Hiroshi Kamigakiuchi, Kazuhiro Iwashita, Ryosuke Mochioka, Nami Goto-Yamamoto
Grapes (Vitis spp.) produce diverse polyphenolic compounds, which are phytochemicals that contribute to human health. In this study, the polyphenolic profiles of the red-purple berries of two wild grape species native to Japan, Vitis ficifolia and V. coignetiae, and their interspecific hybrid cultivars were investigated and compared with the profiles of V. vinifera and V. × labruscana cultivars. Proanthocyanidins (PAs) were present at lower concentrations in both skins and seeds of wild grape species and their hybrid cultivars than those in V...
November 22, 2016: Phytochemistry
https://www.readbyqxmd.com/read/27879624/transcriptome-analysis-identifies-key-candidate-genes-mediating-purple-ovary-coloration-in-asiatic-hybrid-lilies
#14
Leifeng Xu, Panpan Yang, Suxia Yuan, Yayan Feng, Hua Xu, Yuwei Cao, Jun Ming
Lily tepals have a short lifespan. Once the tepals senesce, the ornamental value of the flower is lost. Some cultivars have attractive purple ovaries and fruits which greatly enhance the ornamental value of Asiatic hybrid lilies. However, little is known about the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries. To investigate the transcriptional network that governs purple ovary coloration in Asiatic hybrid lilies, we obtained transcriptome data from green ovaries (S1) and purple ovaries (S2) of Asiatic "Tiny Padhye"...
November 20, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27879622/ultrasound-assisted-extraction-centrifugation-and-ultrafiltration-multistage-process-for-polyphenol-recovery-from-purple-sweet-potatoes
#15
Zhenzhou Zhu, Tian Jiang, Jingren He, Francisco J Barba, Giancarlo Cravotto, Mohamed Koubaa
This work provides an evaluation of an ultrasound-assisted, combined extraction, centrifugation and ultrafiltration process for the optimal recovery of polyphenols. A purple sweet potato (PSP) extract has been obtained using ultrasonic circulating extraction equipment at a power of 840 W, a frequency of 59 kHz and using water as solvent. Extract ultrafiltration, using polyethersulfone (PES), was carried out for the recovery of polyphenol, protein and anthocyanin. Pre-treatment, via the centrifugation of purple sweet potato extract at 2500 rpm over 6 min, led to better polyphenol recovery, with satisfactory protein removal (reused for future purposes), than PSP extract filtration without centrifugation...
November 20, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27878097/population-genetic-isolation-and-limited-connectivity-in-the-purple-finch-haemorhous-purpureus
#16
Colin Bran Alexander Macfarlane, Libby Natola, Mike W Brown, Theresa M Burg
Using a combination of mitochondrial and z-linked sequences, microsatellite data, and spatio-geographic modeling, we examined historical and contemporary factors influencing the population genetic structure of the purple finch (Haemorhous purpureus). Mitochondrial DNA data show the presence of two distinct groups corresponding to the two subspecies, H. p. purpureus and H. p. californicus. The two subspecies likely survived in separate refugia during the last glacial maximum, one on the Pacific Coast and one east of the Rocky Mountains, and now remain distinct lineages with little evidence of gene flow between them...
November 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/27875151/hindsight-encouraging-exploration-through-direct-encoding-of-personal-interaction-history
#17
Mi Feng, Cheng Deng, Evan M Peck, Lane Harrison
Physical and digital objects often leave markers of our use. Website links turn purple after we visit them, for example, showing us information we have yet to explore. These "footprints" of interaction offer substantial benefits in information saturated environments - they enable us to easily revisit old information, systematically explore new information, and quickly resume tasks after interruption. While applying these design principles have been successful in HCI contexts, direct encodings of personal interaction history have received scarce attention in data visualization...
January 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/27875014/a-viologen-perylenediimide-conjugate-as-an-efficient-base-sensor-with-solvochromic-property
#18
Debapratim Das, Bapan Pramanik, Julfikar Hassan Mondal, Nilotpal Singha, Sahnawaz Ahmed, Jyotirmayee Mohanty
A viologen-perylenediimide conjugate, termed as PDEV, is prepared for efficient base sensing. The conjugate is showing solvatochromic behavior as well. The base-sensitivity of viologen is purposefully coupled with the emission property of perylenediimide (PDI) in order to lower the detection limit. PDEV has shown base sensing ability in the ppb level which is at least three orders of magnitude lower than the previously reported sensors. The probe is sensitive toward solvent polarity and generates different shades of colors based on the polarity of the medium (solvent)...
November 22, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/27872504/harmonising-reference-intervals-for-three-calculated-parameters-used-in-clinical-chemistry
#19
REVIEW
David Hughes, Gus Koerbin, Julia M Potter, Nicholas Glasgow, Nic West, Walter P Abhayaratna, Juleen Cavanaugh, David Armbruster, Peter E Hickman
For more than a decade there has been a global effort to harmonise all phases of the testing process, with particular emphasis on the most frequently utilised measurands. In addition, it is recognised that calculated parameters derived from these measurands should also be a target for harmonisation. Using data from the Aussie Normals study we report reference intervals for three calculated parameters: serum osmolality, serum anion gap and albumin-adjusted serum calcium. The Aussie Normals study was an a priori study that analysed samples from 1856 healthy volunteers...
August 2016: Clinical Biochemist. Reviews
https://www.readbyqxmd.com/read/27866619/rhizopus-stolonifer-mediated-biosynthesis-of-biocompatible-cadmium-chalcogenide-quantum-dots
#20
P Mareeswari, J Brijitta, S Harikrishna Etti, C Meganathan, Gobi Saravanan Kaliaraj
We report an efficient method to biosynthesize biocompatible cadmium telluride and cadmium sulphide quantum dots from the fungus Rhizopus stolonifer. The suspension of the quantum dots exhibited purple and greenish-blue luminescence respectively upon UV light illumination. Photoluminescence spectroscopy, X-ray diffraction, and transmission electron microscopy confirms the formation of the quantum dots. From the photoluminescence spectrum the emission maxima is found to be 424 and 476nm respectively. The X-ray diffraction of the quantum dots matches with results reported in literature...
December 2016: Enzyme and Microbial Technology
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