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https://www.readbyqxmd.com/read/29332168/efficient-crispr-cas9-based-genome-editing-in-carrot-cells
#1
Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, Levi G Lowder, Yiping Qi, Rafal Baranski
The first report presenting successful and efficient carrot genome editing using CRISPR/Cas9 system. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas9) is a powerful genome editing tool that has been widely adopted in model organisms recently, but has not been used in carrot-a model species for in vitro culture studies and an important health-promoting crop grown worldwide. In this study, for the first time, we report application of the CRISPR/Cas9 system for efficient targeted mutagenesis of the carrot genome...
January 13, 2018: Plant Cell Reports
https://www.readbyqxmd.com/read/29331904/anthocyanins-in-perilla-plants-and-dried-leaves
#2
Yumi Fujiwara, Miya Kono, Airi Ito, Michiho Ito
High-quality perilla leaves are purple on upper and lower surfaces and have a good aroma. The Japanese Pharmacopoeia specifies the content of essential oils in perilla leaves but not that of anthocyanins. Several reports have described the chemical species of anthocyanins in red perilla, but a complete analysis of anthocyanins in perilla has not been reported. In this study, the anthocyanins in the leaves of cultivated and wild species of perilla and those in commercially available perilla herbs were studied...
January 11, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29331817/vapochromic-behavior-of-mof-for-selective-sensing-of-ethanol
#3
Zhenhua Wang, Qianwang Chen
A MOF material, Co3[Co(CN)6]2 nanoparticles has been prepared for the effective detection of ethanol in vapor phase. When exposed to ethanol vapor, the material was changed from pink to purple, which is easily observed by naked eyes directly. We propose that the ethanol response is due to ethanol molecules entering the pores of the solid, where they alter the coordination geometry, leading to conversion of their Co centers from octahedral to tetrahedral coordination. Significantly, the change is reversible, which make the material reusable without subjecting to dynamic vacuum or slightly warming...
December 30, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29329829/effectiveness-and-safety-of-foam-sclerotherapy-with-5-ethanolamine-oleate-in-the-treatment-of-low-flow-venous-malformations-in-the-head-and-neck-region-a-case-series
#4
M C Ribeiro, S de Mattos Camargo Grossmann, M B F do Amaral, W H de Castro, T P Navarro, R J Procopio, T A da Silva, C de Nazaré Alves de Oliveira Kato, R A Mesquita
The aim of this study was to evaluate the effectiveness and safety of 5% ethanolamine oleate (EO) foam in the treatment of low-flow venous malformations in the head and neck region. Seventeen consecutive patients (six male, 11 female) and 34 low-flow venous malformations were enrolled. The vascular anomalies ranged between 20mm and 80mm in size. The typical clinical indication was a swelling (88.2%) with a purple colour (85.3%); the most frequent location was the tongue (23.5%). Ethanolamine oleate foam was produced via the Tessari method and applied at 10mg per 1cm to the vascular anomalies...
January 9, 2018: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/29329810/facile-microencapsulation-of-olive-oil-in-porous-starch-granules-fabrication-characterization-and-oxidative-stability
#5
Miao Lei, Fang-Cheng Jiang, Jie Cai, Si Hu, Rui Zhou, Gang Liu, Yue-Hui Wang, Hai-Bo Wang, Jing-Ren He, Xin-Gao Xiong
In this study, starch with porous structures derived from purple sweet potato was prepared and used as a food-grade polymer for the microencapsulation of olive oil. The optimal reaction conditions for preparing porous starch were determined to improve its adsorption capacity as effective microcapsule-wall materials. Olive oil was then impregnated in microspheres, and loading ratio was optimized by investigating the restrictive factors, including the mass ratio of olive oil to porous starch, as well as the embedding temperature and time...
January 9, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29329437/association-of-extracellular-dntp-utilization-with-a-gmpap1-like-protein-identified-in-cell-wall-proteomic-analysis-of-soybean-roots
#6
Weiwei Wu, Yan Lin, Pandao Liu, Qianqian Chen, Jiang Tian, Cuiyue Liang
Plant root cell walls are dynamic systems that serve as the first plant compartment responsive to soil conditions, such as phosphorus (P) deficiency. To date, evidence for the regulation of root cell wall proteins (CWPs) by P deficiency remains sparse. In order to gain a better understanding of the roles played by CWPs in the roots of soybean (Glycine max) in adaptation to P deficiency, we conducted an iTRAQ (isobaric tag for relative and absolute quantitation) proteomic analysis. A total of 53 CWPs with differential accumulation in response to P deficiency were identified...
January 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29329278/t-dna-tagging-based-gain-of-function-of-oshkt1-4-reinforces-na-exclusion-from-leaves-and-stems-but-triggers-na-toxicity-in-roots-of-rice-under-salt-stress
#7
Yuuka Oda, Natsuko I Kobayashi, Keitaro Tanoi, Jian Feng Ma, Yukiko Itou, Maki Katsuhara, Takashi Itou, Tomoaki Horie
The high affinity K⁺ transporter 1;4 (HKT1;4) in rice (Oryza sativa), which shows Na⁺ selective transport with little K⁺ transport activity, has been suggested to be involved in reducing Na in leaves and stems under salt stress. However, detailed physiological roles of OsHKT1;4 remain unknown. Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice...
January 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29325213/a-new-colorimetric-platform-for-protein-detection-based-on-recognition-induced-cascade-of-polymeric-nanoparticles-disassembly
#8
Fang Fang, Jin Liu, Yushu Li, Junjiao Yang, Jing Yang
Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the molecular recognition between protein and the ligand on the surface of the polymeric micelles as the driving force to trigger the readout of the detection signal. The polymeric micelles formed via the self-assembly of the amphiphilic block polymer biotin-labeled poly(ethylene glycol)-block-poly(3-acryl aminophenylboronic acid) are endowed with colorful feature by incorporation of alizarin red S (ARS) into the hydrophobic core...
January 11, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29322139/effects-of-polysaccharides-from-purple-sweet-potatoes-on-immune-response-and-gut-microbiota-composition-in-normal-and-cyclophosphamide-treated-mice
#9
Chao Tang, Jian Sun, Bo Zhou, Changhai Jin, Jun Liu, Juan Kan, Chunlu Qian, Nianfeng Zhang
In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage. The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6. As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice...
January 11, 2018: Food & Function
https://www.readbyqxmd.com/read/29321449/a-simple-paper-based-colorimetric-device-for-rapid-and-sensitive-urinary-oxalate-determinations
#10
Piyakorn Worramongkona, Kanyarat Seeda, Phatharatanathorn Phansomboon, Nalin Ratnarathorn, Orawon Chailapakul, Wijitar Dungchai
In this work, a simple and inexpensive paper-based colorimetric device (cPAD) was developed for oxalate measurements. The colorimetric assay is based on the formation of formazan via the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by oxalate decarboxylase and formate dehydrogenase on the paper device. After the sample was spotted on the device, MTT changed color from yellow to purple, and the purple color intensity correlated with the oxalate concentration. The quantitative detection of oxalate ranged from 10 - 1000 μM, with a linear equation, y = 0...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321265/contribution-of-low-temperature-single-molecule-techniques-to-structural-issues-of-pigment-protein-complexes-from-photosynthetic-purple-bacteria
#11
REVIEW
Alexander Löhner, Richard Cogdell, Jürgen Köhler
As the electronic energies of the chromophores in a pigment-protein complex are imposed by the geometrical structure of the protein, this allows the spectral information obtained to be compared with predictions derived from structural models. Thereby, the single-molecule approach is particularly suited for the elucidation of specific, distinctive spectral features that are key for a particular model structure, and that would not be observable in ensemble-averaged spectra due to the heterogeneity of the biological objects...
January 2018: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29317677/isolation-and-characterization-of-key-genes-that-promote-flavonoid-accumulation-in-purple-leaf-tea-camellia-sinensis-l
#12
Xiujuan He, Xuecheng Zhao, Liping Gao, Xingxing Shi, Xinlong Dai, Yajun Liu, Tao Xia, Yunsheng Wang
There were several high concentrations of flavonoid components in tea leaves that present health benefits. A novel purple-leaf tea variety, 'Mooma1', was obtained from the natural hybrid population of Longjing 43 variety. The buds and young leaves of 'Mooma1' were displayed in bright red. HPLC and LC-MS analysis showed that anthocyanins and O-Glycosylated flavonols were remarkably accumulated in the leaves of 'Mooma1', while the total amount of catechins in purple-leaf leaves was slightly decreased compared with the control...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29316629/increase-of-salt-tolerance-in-carbon-starved-cells-of-rhodopseudomonas-palustris-depending-on-photosynthesis-or-respiration
#13
Sawa Wasai, Nanako Kanno, Katsumi Matsuura, Shin Haruta
Bacteria in natural environments are frequently exposed to nutrient starvation and survive against environmental stresses under non-growing conditions. In order to determine the energetic influence on survivability during starvation, changes in salt tolerance were investigated using the purple photosynthetic bacterium Rhodopseudomonas palustris after carbon starvation under photosynthetic conditions in comparison with anaerobic and aerobic dark conditions. Tolerance to a treatment with high concentration of salt (2...
January 6, 2018: Microorganisms
https://www.readbyqxmd.com/read/29315241/synthesis-of-novel-reactive-disperse-silicon-containing-dyes-and-their-coloring-properties-on-silicone-rubbers
#14
Ning Yu, Shufen Zhang, Bingtao Tang, Wei Ma, Jinjing Qiu
Novel red and purple reactive disperse silicon-containing dyes were designed and synthesized using p-nitroaniline and 6-bromo-2,4-dinitro-aniline as diazonium components, the first condensation product of cyanuric chloride and 3-(N,N-diethyl)amino-aniline as coupling component, and 3-aminopropylmethoxydimethylsilane, 3-aminopropylmethyldimethoxysilane, and 3-aminopropyltrimethoxysilane as silicone reactive agents. These dyes were characterized by UV-Vis, ¹H-NMR, FT-IR, and MS. The obtained reactive disperse silicon-containing dyes were used to color silicone rubbers and the color fastness of the dyes were evaluated...
January 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29310850/utilization-of-distillery-wastewater-for-hydrogen-production-in-one-stage-and-two-stage-processes-involving-photofermentation
#15
Tatyana Laurinavichene, Darya Tekucheva, Kestutis Laurinavichius, Anatoly Tsygankov
In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H2 yield of 17.6L/L of distillery waste with chemical oxygen demand 40g/L. It is equivalent to 205kJ/L distillery wastewater and corresponds to recovery of approximately 4-8% of energy consumed during ethanol production. Optimal performance of photofermentation was observed at 20% concentration of pre-fermented distillery waste...
March 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29306041/rubbery-purple-and-red-lesions-in-the-gi-tract-a-case-of-gi-tract-kaposi-sarcoma
#16
Tarek Nammour, Michael Dagher, Jana G Hashash
No abstract text is available yet for this article.
January 3, 2018: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29304411/rapid-screening-of-brain-penetrable-antioxidants-from-natural-products-by-blood-brain-barrier-specific-permeability-assay-combined-with-dpph-recognition
#17
Junfeng Zhu, Xiaojiao Yi, Jinghui Zhang, Shuqing Chen, Yongjiang Wu
Screening brain-penetrable antioxidants from natural products is a promising way for neuroprotective drug discovery. However, there is no screening methodology enables simultaneous investigation of antioxidant activity and blood-brain barrier (BBB) permeability of compounds from complex samples. Here we propose a novel strategy by combining BBB specific parallel artificial membrane permeability assay with 1,1-diphenyl-2-picrylhydrazyl recognition (BBB-PAMPA-DPPH) to achieve rapid multicomponent screening. First, BBB specific artificial membrane was constructed to separate the compounds with high BBB permeability in herbal extracts...
December 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29304273/effect-of-temperature-and-hydration-level-on-purple-membrane-dynamics-studied-using-broadband-dielectric-spectroscopy-from-sub-ghz-to-thz-regions
#18
Naoki Yamamoto, Shota Ito, Masahiro Nakanishi, Eri Chatani, Keiichi Inoue, Hideki Kandori, Keisuke Tominaga
To investigate the effects of temperature and hydration on the dynamics of purple membrane (PM), we measured the broadband complex dielectric spectra from 0.5 GHz to 2.3 THz using a vector network analyzer and terahertz time-domain spectroscopy from 233 to 293 K. In the lower temperature region down to 83 K, the complex dielectric spectra in the THz region were also obtained. The complex dielectric spectra were analyzed through curve fitting using several model functions. We found that the hydrated states of one relaxational mode, which was assigned as the coupled motion of water molecules with the PM surface, began to overlap with the THz region at approximately 230 K...
January 5, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29304111/combined-bulked-segregant-sequencing-and-traditional-linkage-analysis-for-identification-of-candidate-gene-for-purple-leaf-sheath-in-maize
#19
Pengcheng Li, Cancan Du, Yingying Zhang, Shuangyi Yin, Enying Zhang, Huimin Fang, Dezhou Lin, Chenwu Xu, Zefeng Yang
Anthocyanin accumulation in various maize tissues plays important roles in plant growth and development. In addition, some color-related traits can be used as morphological markers in conventional maize breeding processes and purity identification of hybrid seeds. Here, we noticed that the leaf sheath color was controlled by a dominant gene, because purple (PSH) and green leaf sheaths (GSH) were separated at a ratio of 3:1 in an F2 population. To map the gene, an F2 and a recombinant inbred line (RIL) population were derived from a cross between inbred line T877 (PSH) and DH1M (GSH)...
2018: PloS One
https://www.readbyqxmd.com/read/29302040/light-tuned-selective-photosynthesis-of-azo-and-azoxy-aromatics-using-graphitic-c3n4
#20
Yitao Dai, Chao Li, Yanbin Shen, Tingbin Lim, Jian Xu, Yongwang Li, Hans Niemantsverdriet, Flemming Besenbacher, Nina Lock, Ren Su
Solar-driven photocatalysis has attracted significant attention in water splitting, CO2 reduction and organic synthesis. The syntheses of valuable azo- and azoxyaromatic dyes via selective photoreduction of nitroaromatic compounds have been realised using supported plasmonic metal nanoparticles at elevated temperatures (≥90 °C); however, the high cost, low efficiency and poor selectivity of such catalyst systems at room temperature limit their application. Here we demonstrate that the inexpensive graphitic C3N4 is an efficient photocatalyst for selective syntheses of a series of azo- and azoxy-aromatic compounds from their corresponding nitroaromatics under either purple (410 nm) or blue light (450 nm) excitation...
January 4, 2018: Nature Communications
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