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https://www.readbyqxmd.com/read/28236074/interaction-of-the-signaling-state-analog-and-the-apoprotein-form-of-the-orange-carotenoid-protein-with-the-fluorescence-recovery-protein
#1
Marcus Moldenhauer, Nikolai N Sluchanko, Neslihan N Tavraz, Cornelia Junghans, David Buhrke, Mario Willoweit, Leonardo Chiappisi, Franz-Josef Schmitt, Vladana Vukojević, Evgeny A Shirshin, Vladimir Y Ponomarev, Vladimir Z Paschenko, Michael Gradzielski, Eugene G Maksimov, Thomas Friedrich
Photoprotection in cyanobacteria relies on the interplay between the orange carotenoid protein (OCP) and the fluorescence recovery protein (FRP) in a process termed non-photochemical quenching, NPQ. Illumination with blue-green light converts OCP from the basic orange state (OCP(O)) into the red-shifted, active state (OCP(R)) that quenches phycobilisome (PBs) fluorescence to avoid excessive energy flow to the photosynthetic reaction centers. Upon binding of FRP, OCP(R) is converted to OCP(O) and dissociates from PBs; however, the mode and site of OCP(R)/FRP interactions remain elusive...
February 24, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28230808/genome-sequence-of-rhodoferax-antarcticus-ant-br-t-a-psychrophilic-purple-nonsulfur-bacterium-from-an-antarctic-microbial-mat
#2
Jennifer M Baker, Carli J Riester, Blair M Skinner, Austin W Newell, Wesley D Swingley, Michael T Madigan, Deborah O Jung, Marie Asao, Min Chen, Patrick C Loughlin, Hao Pan, Yuankui Lin, Yaqiong Li, Jacob Shaw, Mindy Prado, Chris Sherman, Joseph Kuo-Hsiang Tang, Robert E Blankenship, Tingting Zhao, Jeffrey W Touchman, W Matthew Sattley
Rhodoferax antarcticus is an Antarctic purple nonsulfur bacterium and the only characterized anoxygenic phototroph that grows best below 20 °C. We present here a high-quality draft genome of Rfx. antarcticus strain ANT.BR(T), isolated from an Antarctic microbial mat. The circular chromosome (3.8 Mbp) of Rfx. antarcticus has a 59.1% guanine + cytosine (GC) content and contains 4036 open reading frames. In addition, the bacterium contains a sizable plasmid (198.6 kbp, 48.4% GC with 226 open reading frames) that comprises about 5% of the total genetic content...
February 21, 2017: Microorganisms
https://www.readbyqxmd.com/read/28224340/effects-of-land-disturbance-on-runoff-and-sediment-yield-after-natural-rainfall-events-in-southwestern-china
#3
Xiaomeng Guo, Tianyang Li, Binghui He, Xiaorong He, Yun Yao
Severe soil erosion occurs in southwestern China owing to the large expanses of urbanization and sloping land. This field monitoring study was conducted to record the rainfall events, runoff, and sediment yield in 20-, 40-, and 60-m plots under conditions of artificial disturbance or natural restoration in the purple soil area of southwestern China. The study took place during the rainy season, and the plots were situated on a 15° slope. The results showed that rainstorms and heavy rainstorms generated runoff and sediment yield...
February 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28217828/photophysics-of-egfp-e222h-mutant-with-comparisons-to-model-chromophores-excited-state-pk-s-progressions-quenching-and-exciton-interaction
#4
William Kirk, Thomas Allen, Elena Atanasova, William Wessels, Janet Yao, Franklyn Prendergast
A novel version of the well-known and commercially successful Green Fluorescent Protein (GFP) variant known as EGFP, with an introduced E222H mutation, was produced in this laboratory. Given the current state of hypotheses about the role of glutamate 222, and the observed dominance of the phenolate absorption with an E222H variant observed from earlier study, the new mutant was considered a natural choice to investigate more fully the acid-base behavior of the chromophore in absorption and fluorescence. The bulk of this investigation concerns fitting the excitation, emission and absorption spectra to vibrational progressions of a novel 'q-deformed' type at various values of pH, and protein concentration...
February 20, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28212707/astrobiological-implications-of-dim-light-phototrophy-in-deep-sea-red-clays
#5
Anindita Das, Tanya Singh, P A LokaBharathi, Prashant K Dhakephalkar, Sweta Mallik, Pranav R Kshirsagar, N H Khadge, B Nagender Nath, Satadru Bhattacharya, Aditya Kumar Dagar, Prabhjot Kaur, Dwijesh Ray, Anil D Shukla, Christabelle E G Fernandes, Sheryl O Fernandes, Tresa Remya A Thomas, Mamatha S S, Babu Shashikant Mourya, Ram Murti Meena
Red clays of Central Indian Basin (CIB) under influence of trace of Rodriguez Triple Junction exhibited chemoautotrophy, low temperature hydrothermal alterations and photoautotrophic potential. Seamount flank TVBC-08, hosting such signatures revealed dominance of aerobic anoxygenic phototroph Erythrobacter, with 93% of total 454 pyrosequencing tags. Subsequently, enrichments for both aerobic (Erythrobacter) and anaerobic anoxygenic phototrophs (green and purple sulphur bacteria) under red and white LED light illumination, with average irradiance 30...
February 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28211680/two-way-gold-nanoparticle-label-free-sensing-of-specific-sequence-and-small-molecule-targets-using-switchable-concatemers
#6
Longjiao Zhu, Xiangli Shao, Yunbo Luo, Kunlun Huang, Wentao Xu
A two-way colorimetric biosensor based on unmodified gold nanoparticles (GNPs) and a switchable double-stranded DNA (dsDNA) concatemer have been demonstrated. Two hairpin probes (H1&H2) were first designed that provided the fuels to assemble the dsDNA concatemers via hybridization chain reaction (HCR). A functional hairpin (FH) was rationally designed to recognize the target sequences. All the hairpins contained single-stranded DNA (ssDNA) loop and sticky-ends to prevent GNPs from salt-induced aggregation. In the presence of target sequence, the capture probe blocked in the FH recognizes the target to form a duplex DNA, which causes the release of the initiator probe by FH conformational change...
February 17, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28205063/excitation-energy-transfer-from-the-bacteriochlorophyll-soret-band-to-carotenoids-in-the-lh2-light-harvesting-complex-from-ectothiorhodospira-haloalkaliphila-is-negligible
#7
A P Razjivin, E P Lukashev, V O Kompanets, V S Kozlovsky, A A Ashikhmin, S V Chekalin, A A Moskalenko, V Z Paschenko
Pathways of intramolecular conversion and intermolecular electronic excitation energy transfer (EET) in the photosynthetic apparatus of purple bacteria remain subject to debate. Here we experimentally tested the possibility of EET from the bacteriochlorophyll (BChl) Soret band to the singlet S2 level of carotenoids using femtosecond pump-probe measurements and steady-state fluorescence excitation and absorption measurements in the near-ultraviolet and visible spectral ranges. The efficiency of EET from the Soret band of BChl to S2 of the carotenoids in light-harvesting complex LH2 from the purple bacterium Ectothiorhodospira haloalkaliphila appeared not to exceed a few percent...
February 16, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28201992/high-flavonoid-accompanied-with-high-starch-accumulation-triggered-by-nutrient-starvation-in-bioenergy-crop-duckweed-landoltia-punctata
#8
Xiang Tao, Yang Fang, Meng-Jun Huang, Yao Xiao, Yang Liu, Xin-Rong Ma, Hai Zhao
BACKGROUND: As the fastest growing plant, duckweed can thrive on anthropogenic wastewater. The purple-backed duckweed, Landoltia punctata, is rich in starch and flavonoids. However, the molecular biological basis of high flavonoid and low lignin content remains largely unknown, as does the best method to combine nutrients removed from sewage and the utilization value improvement of duckweed biomass. RESULTS: A combined omics study was performed to investigate the biosynthesis of flavonoid and the metabolic flux changes in L...
February 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28201986/the-origin-of-chow-chows-in-the-light-of-the-east-asian-breeds
#9
Hechuan Yang, Guodong Wang, Meng Wang, Yaping Ma, Tingting Yin, Ruoxi Fan, Hong Wu, Li Zhong, David M Irwin, Weiwei Zhai, Yaping Zhang
BACKGROUND: East Asian dog breeds are one of the most ancient groups of dogs that radiated after the domestication of the dog and represent the most basal lineages of dog evolution. Among these, the Chow Chow is an ancient breed that embodies very distinct morphological and physiological features, such as sturdy build, dense coat, and blue/purple tongue. RESULTS: Using a Restricted site Associated DNA (RAD) sequencing approach, we sequenced the genomes of nine Chow Chows from China...
February 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28199929/identification-of-natural-red-and-purple-dyes-on-textiles-by-fiber-optics-reflectance-spectroscopy
#10
M A Maynez-Rojas, E Casanova-González, J L Ruvalcaba-Sil
Understanding dye chemistry and dye processes is an important issue for studies of cultural heritage collections and science conservation. Fiber Optics Reflectance Spectroscopy (FORS) is a powerful technique, which allows preliminary dye identification, causing no damage or mechanical stress on the artworks subjected to analysis. Some information related to specific light scattering and absorption can be obtained in the UV-visible and infrared range (300-1400nm) and it is possible to discriminate the kind of support fiber in the near infrared region (1000-2500nm)...
February 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28196888/sulfide-responsive-transcriptional-repressor-sqrr-functions-as-a-master-regulator-of-sulfide-dependent-photosynthesis
#11
Takayuki Shimizu, Jiangchuan Shen, Mingxu Fang, Yixiang Zhang, Koichi Hori, Jonathan C Trinidad, Carl E Bauer, David P Giedroc, Shinji Masuda
Sulfide was used as an electron donor early in the evolution of photosynthesis, with many extant photosynthetic bacteria still capable of using sulfur compounds such as hydrogen sulfide (H2S) as a photosynthetic electron donor. Although enzymes involved in H2S oxidation have been characterized, mechanisms of regulation of sulfide-dependent photosynthesis have not been elucidated. In this study, we have identified a sulfide-responsive transcriptional repressor, SqrR, that functions as a master regulator of sulfide-dependent gene expression in the purple photosynthetic bacterium Rhodobacter capsulatus SqrR has three cysteine residues, two of which, C41 and C107, are conserved in SqrR homologs from other bacteria...
February 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28193578/a-food-based-approach-that-targets-interleukin-6-a-key-regulator-of-chronic-intestinal-inflammation-and-colon-carcinogenesis
#12
Abigail Sido, Sridhar Radhakrishnan, Sung Woo Kim, Elisabeth Eriksson, Frank Shen, Qunhua Li, Vadiraja Bhat, Lavanya Reddivari, Jairam K P Vanamala
Studies have shown a causal link between high-calorie diet (HCD) and colon cancer. However, molecular mechanisms are not fully elucidated. To understand etiology of HCD-induced colon carcinogenesis, we screened 10 pathways linked to elevated colonic cell proliferation and chronic inflammation in an HCD-consuming human-relevant pig model. We observed elevated colonic mucosal interleukin-6 (IL-6) expression in HCD-consuming pigs compared to standard diet controls (SD, P=.04), and IL-6 strongly correlated with Ki-67 proliferative index and zone, early biomarkers of colon cancer risk (r=0...
January 28, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28190594/separation-of-betacyanins-from-purple-flowers-of-gomphrena-globosa-l-by-ion-pair-high-speed-counter-current-chromatography
#13
Aneta Spórna-Kucab, Joanna Jagodzińska, Sławomir Wybraniec
Betacyanins, known as antioxidants and chemopreventive natural compounds with colourful properties, were extracted from purple flowers of Gomphrena globosa L. belonging to the Amaranthaceae family and separated for the first time by ion-pair high-speed counter-current chromatography (HSCCC). The pigments were detected by LC-DAD-ESI-MS/MS technique. Separation of betacyanins (300mg) by HSCCC was accomplished in four solvent systems: tert-butyl methyl ether - butanol - acetonitrile - water (0.7% and 1.0% HFBA - heptafluorobutyric acid - system I and III) and tert-butyl methyl ether - butanol - methanol - water (0...
March 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28186362/effects-of-the-herbicide-triclopyr-on-metamorphic-northern-red-legged-frogs
#14
Amy E Yahnke, Christian E Grue, Marc P Hayes, Schuyler Pearman-Gillman
Aquatic herbicides are used to manage invasive emergent plants in and around wetlands. Metamorphic frogs that emerge during the aquatic weed management season may be at risk of herbicide exposure. Metamorphic northern red-legged frogs (Rana aurora) were exposed to a triclopyr tank mix labelled for control of broadleaf emergent aquatic weeds such as invasive purple loosestrife (Lythrum salicaria). The tank mix consisted of Renovate® 3 (triclopyr triethylamine salt [TEA] 44.4%), the modified vegetable oil surfactant Competitor®, and marker dye Hi-Light®...
February 10, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28180989/development-of-a-new-colorimetric-assay-for-detection-of-bisphenol-a-in-aqueous-media-using-green-synthesized-silver-chloride-nanoparticles-experimental-and-theoretical-study
#15
Shiva Khalililaghab, Safieh Momeni, Maryam Farrokhnia, Iraj Nabipour, Sadegh Karimi
In the present study, a cost-effective, green and simple synthesis method was applied for preparation of stable silver chloride nanoparticles (AgCl-NPs). The method was done by forming AgCl-NPs from Ag(+) ions using aqueous extract of brown algae (Sargassum boveanum) obtained from the Persian Gulf Sea. This extract served as capping agent during the formation of AgCl-NPs. Creation of AgCl-NPs was confirmed by UV-visible spectroscopy, powder X-ray diffraction, energy-dispersive X-ray spectroscopy, and high-resolution transmission electron microscopy, while the morphology and size analyses were characterized using high-resolution transmission electron microscopy and dynamic light scattering...
February 8, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28179521/egg-discrimination-along-a-gradient-of-natural-variation-in-eggshell-coloration
#16
Daniel Hanley, Tomáš Grim, Branislav Igic, Peter Samaš, Analía V López, Matthew D Shawkey, Mark E Hauber
Accurate recognition of salient cues is critical for adaptive responses, but the underlying sensory and cognitive processes are often poorly understood. For example, hosts of avian brood parasites have long been assumed to reject foreign eggs from their nests based on the total degree of dissimilarity in colour to their own eggs, regardless of the foreign eggs' colours. We tested hosts' responses to gradients of natural (blue-green to brown) and artificial (green to purple) egg colours, and demonstrate that hosts base rejection decisions on both the direction and degree of colour dissimilarity along the natural, but not artificial, gradient of egg colours...
February 8, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28178635/br-padap-embedded-in-cellulose-acetate-electrospun-nanofibers-colorimetric-sensor-strips-for-visual-uranyl-recognition
#17
Lin Hu, Xue-Wu Yan, Qi Li, Xue-Ji Zhang, Dan Shan
In this work, a new visual colorimetric strip based on cellulose acetate nanofiber mats modified by 2-(5-Bromo-2-pyridylazo)-5-(diethylamino) phenol was successfully prepared via electrospinning technology. The prepared colorimetric strip showed high sensitivity towards UO2(2+) with the yellow-to-purple color change signal. Upon the optimal conditions of solution pH at 6.0 and response time for 80min, the detection limit for UO2(2+) can reach 50 ppb. Moreover, the strip also exhibited excellent anti-interference ability in the presence of other metal ions...
January 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28168649/combined-orcein-and-martius-scarlet-blue-omsb-staining-for-qualitative-and-quantitative-analyses-of-atherosclerotic-plaques-in-brachiocephalic-arteries-in-apoe-ldlr-mice
#18
Mariusz Gajda, Agnieszka Jasztal, Tomasz Banasik, Ewa Jasek-Gajda, Stefan Chlopicki
Numerous cellular and extracellular components should be analyzed in sections of atherosclerotic plaques to assess atherosclerosis progression and vulnerability. Here, we combined orcein (O) staining for elastic fibers and martius scarlet blue (MSB) polychrome to visualize various morphological contents of plaque in brachiocephalic arteries (BCA) of apoE/LDLR(-/-) mice. Elastic fibers (including broken elastic laminae and 'buried' fibrous caps) were stained purple and they could be easily distinguished from collagen fibers (blue)...
February 6, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28157580/physiological-highlights-of-manganese-toxicity-symptoms-in-soybean-plants-mn-toxicity-responses
#19
Elcio Ferreira Santos, José Mateus Kondo Santini, Amanda Pereira Paixão, Enes Furlani Júnior, José Lavres, Marcelo Campos, André Rodrigues Dos Reis
Manganese (Mn) is an essential element for plants; however, high concentrations in certain soil conditions can cause toxicity symptoms in the plant tissue. Here, we describe Mn toxicity symptoms and Mn toxicity responses in soybean plants. Soybean plants exposed to excess Mn showed reductions in the CO2 assimilation rate and stomatal conductance, which in turn resulted in decreased shoot biomass. Furthermore, peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activity were higher in plants grown with the highest Mn concentration...
January 25, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28155905/multipath-colourimetric-assay-for-copper-ii-ions-utilizing-marr-functionalized-gold-nanoparticles
#20
Yulong Wang, Limin Wang, Zhenhe Su, Juanjuan Xue, Jinbo Dong, Cunzheng Zhang, Xiude Hua, Minghua Wang, Fengquan Liu
We use the multiple antibiotic resistance regulator (MarR), as a highly selective biorecognition elements in a multipath colourimetric sensing strategy for the fast detection of Cu(2+) in water samples. The colourimetric assay is based on the aggregation of MarR-coated gold nanoparticles in the presence of Cu(2+) ions, which induces a red-to-purple colour change of the solution. The colour variation in the gold nanoparticle aggregation process can be used for qualitative and quantitative detection of Cu(2+) by the naked eye, and with UV-vis and smartphone-based approaches...
February 3, 2017: Scientific Reports
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