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https://www.readbyqxmd.com/read/28231528/identification-of-uv-absorbing-extractables-from-rubber-closures-used-in-containers-of-injectable-powder-and-safety-assessment-of-leachables-in-the-drug
#1
Yulei Wei, Ying Wu, Tingli Zhu, Zhiyan Li, Yilan Zhang
Rubber closures have been of great concern to regulatory authorities on account of their potential safety risks to patients. The aim of our work is to provide part of data about the compatibility of the injectable powder and its packaging materials for the drug registration. In this report, methodologies were established to study the system of the preparation. Firstly, three major extractables were isolated by semi-preparative HPLC method combined with silica gel-based chromatographic methods. NMR spectra including 1D NMR ((1)H, (13)C, DEPT135) and 2D NMR (COSY, HSQC, HMBC) were introduced to identify the extractables, besides HPLC, GC-MS, ESI-MS/MS and HRMS...
February 16, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28231147/applying-least-absolute-shrinkage-selection-operator-and-akaike-information-criterion-analysis-to-find-the-best-multiple-linear-regression-models-between-climate-indices-and-components-of-cow-s-milk
#2
Mohammad Reza Marami Milani, Andreas Hense, Elham Rahmani, Angelika Ploeger
This study focuses on multiple linear regression models relating six climate indices (temperature humidity THI, environmental stress ESI, equivalent temperature index ETI, heat load HLI, modified HLI (HLI new), and respiratory rate predictor RRP) with three main components of cow's milk (yield, fat, and protein) for cows in Iran. The least absolute shrinkage selection operator (LASSO) and the Akaike information criterion (AIC) techniques are applied to select the best model for milk predictands with the smallest number of climate predictors...
July 23, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230759/analysis-of-the-total-biflavonoids-extract-from-selaginella-doederleinii-by-hplc-qtof-ms-and-its-in-vitro-and-in-vivo-anticancer-effects
#3
Hong Yao, Bing Chen, Yanyan Zhang, Huigen Ou, Yuxiang Li, Shaoguang Li, Peiying Shi, Xinhua Lin
Selaginella doederleinii Hieron has been traditionally used as a folk antitumor herbal medicine in China. In this paper, the phytochemical components of the total biflavonoids extract from S. doederleinii were studied by using high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF MS/MS) in negative ion mode, and their in vitro and in vivo anticancer effects were evaluated. Four types of biflavonoids from S. doederleinii, including IC3'-IIC8'', IC3'-IIC6'', IC3'-IIC3''', and C-O linked biflavonoids were examined originally using QTOF MS/MS...
February 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28229436/purification-and-characterization-of-a-novel-ubiquitin-like-antitumour-protein-with-hemagglutinating-and-deoxyribonuclease-activities-from-the-edible-mushroom-ramaria-botrytis
#4
Rong Zhou, Ya-Jie Han, Min-Hui Zhang, Ke-Ren Zhang, Tzi Bun Ng, Fang Liu
A novel ubiquitin-like antitumour protein (RBUP) was isolated from fruiting bodies of the edible mushroom Ramaria botrytis. The protein was isolated with a purification protocol involving ion exchange chromatography on DEAE-Sepharose fast flow and gel filtration on Sephadex G-75. SDS-PAGE, Native-PAGE and ultracentrifugation analysis disclosed that RBUP was a monomeric protein with a molecular weight of 18.5 kDa. ESI-MS/MS demonstrated that it shared 69% amino acid sequence similarity with Coprinellus congregates ubiquitin (gi|136667)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28226450/identifying-epileptic-source-location-and-extent-an-iterative-sparse-electromagnetic-source-imaging-algorithm
#5
Abbas Sohrabpour, Yunfeng Lu, Gregory Worrell, Bin He, Abbas Sohrabpour, Yunfeng Lu, Gregory Worrell, Bin He, Abbas Sohrabpour, Yunfeng Lu, Gregory Worrell, Bin He
In this paper we have introduced a novel electromagnetic source imaging (ESI) technique and demonstrated its validity and excellent performance in imaging the location and extent of underlying epileptic sources in patients suffering from focal epilepsy. The proposed algorithm employs ideas from sparse signal processing literature and convex optimization theories to improve source imaging results obtained from scalp-recorded electroencephalogram (EEG). EEG source imaging results generally use subjective methods to determine the extent of the underlying brain activity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225615/structural-characterization-and-in-vitro-fermentation-of-%C3%AE-mannooligosaccharides-produced-from-locust-bean-gum-by-gh-26-endo-%C3%AE-1-4-mannanase-manb-1601
#6
Praveen Kumar Srivastava, Deepesh Panwar, Harish Prashanth Kv, Mukesh Kapoor
Size exclusion chromatography of β-mannooligosaccharides (β-MOS) mixture, obtained from ManB-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with varied degree of polymerization (DP 2, 3 and 5). The oligosaccharides were structurally [ESI-MS, FTIR, XRD, TGA and NMR (1H and 13C)] and functionally (in vitro fermentation) characterized. DP2 oligosaccharide was composed of two species (A) mannopyranose β-1,4 mannopyranose and (B) α-1,6-galactosyl-mannopyranose, while DP3 oligosaccharide showed presence of only one species i...
February 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28224358/synthesis-characterization-and-dna-binding-studies-of-hydroxyl-functionalized-platinum-ii-salphen-complexes
#7
Shahratul Ain Mohd Sukri, Lee Yook Heng, Nurul Huda Abd Karim
The platinum(II) salphen complex N,N'-Bis-4-(hydroxysalicylidene)-phenylenediamine-platinum(II); (1) and its two derivatives containing hydroxyl functionalized side chains N,N'-bis-[4-[[1-(2-hydroxyethoxy)] salicylidene] phenylenediamine-platinum(II); (2) and N,N'-bis-[4-[[1-(3-hydroxypropoxy)] salicylidene] phenylenediamine-platinum(II); (3) were synthesized and characterized. The structures of the complexes were confirmed by (1)H and (13)C NMR spectroscopy, FTIR, ESI-MS and CHN elemental analyses. The effects of the hydroxyl substituent on the spectral properties and the DNA binding behaviors of the Pt(II) complexes were explored...
February 21, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28224249/signal-enhancement-in-the-hplc-esi-ms-ms-analysis-of-spironolactone-and-its-metabolites-using-hfip-and-nh4f-as-eluent-additives
#8
Kalev Takkis, Rudolf Aro, Lenne-Triin Kõrgvee, Heili Varendi, Jana Lass, Koit Herodes, Karin Kipper
This paper describes an LC-MS/MS method to determine the concentration of spironolactone and its metabolites 7-alpha-methylthiospironolactone and canrenone in blood plasma samples. The resulting assay is simple (using protein precipitation for sample preparation) and sensitive (the lower limit of quantification is close to 0.5 ng/ml) while requiring only 50 μl of plasma, making it especially suitable for analyzing samples obtained from pediatric and neonatal patients where sample sizes are limited. The sensitivity is achieved by using ammonium fluoride as an eluent additive, which in our case amplifies the signal from our analytes in the plasma solution on average about 70 times...
February 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28224106/impact-of-high-dose-vitamin-c-on-platelet-function
#9
Bassem M Mohammed, Kimberly W Sanford, Bernard J Fisher, Erika J Martin, Daniel Contaifer, Urszula Osinska Warncke, Dayanjan S Wijesinghe, Charles E Chalfant, Donald F Brophy, Alpha A Fowler Iii, Ramesh Natarajan
AIM: To examine the effect of high doses of vitamin C (VitC) on ex vivo human platelets (PLTs). METHODS: Platelet concentrates collected for therapeutic or prophylactic transfusions were exposed to: (1) normal saline (control); (2) 0.3 mmol/L VitC (Lo VitC); or (3) 3 mmol/L VitC (Hi VitC, final concentrations) and stored appropriately. The VitC additive was preservative-free buffered ascorbic acid in water, pH 5.5 to 7.0, adjusted with sodium bicarbonate and sodium hydroxide...
February 4, 2017: World Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28223005/schisandra-lignans-production-regulated-by-different-bioreactor-type
#10
Agnieszka Szopa, Adam Kokotkiewicz, Maria Luczkiewicz, Halina Ekiert
Schisandra chinensis (Chinese magnolia vine) is a rich source of therapeutically relevant dibenzocyclooctadiene lignans with anticancer, immunostimulant and hepatoprotective activities. In the present work, shoot cultures of S. chinensis were grown in different types of bioreactors with the aim to select a system suitable for the large scale in vitro production of schisandra lignans. The cultures were maintained in Murashige-Skoog (MS) medium supplemented with 3mg/l 6-benzylaminopurine (BA) and 1mg/l 1-naphthaleneacetic acid (NAA)...
February 13, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28221758/synthesis-and-fabrication-of-collagen-coated-ostholamide-electrospun-nanofiber-scaffold-for-wound-healing
#11
Subramani Kandhasamy, Sathiamurthi Perumal, Balaraman Madhan, Narayanan Umamaheswari, Javaid Ahmad Banday, Paramasivan Thirumalai Perumal, Vichangal Pridiuldi Santhanakrishnan
A novel scaffold for effective wound healing treatment was developed utilizing natural product bearing collagen based biocompatible electro-spun nanofibres. Initially, ostholamide (OSA) was synthesized from osthole (a natural coumarin), characterized by 1H, 13C, DEPT-135 NMR, ESI-MS and FT-IR spectroscopy analysis. OSA was incorporated into Polyhydroxybutyrate (PHB) and Gelatin (GEL), which serve as templates for electro-spun nanofibers. The coating of OSA-PHB-GEL nanofibers with collagen resulted in PHB-GEL-OSA-COL nanofibrous scaffold which mimics extra cellular matrix and serves as an effective biomaterial for tissue engineering applications, especially for wound healing...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220590/biosynthesis-and-mass-spectral-fragmentation-pathways-of-13-c-and-15-n-labeled-cytochalasin-d-produced-by-xylaria-arbuscula
#12
Luciana da Silva Amaral, Taicia Pacheco Fill, Luiz Fernando Arruda Santos, Edson Rodrigues-Filho
The fungus Xylaria arbuscula was isolated as an endophyte from Cupressus lusitanica and has shown to be a prominent producer of cytochalasans, mainly cytochalasin C, D and Q. Cytochalasans comprise an important class of fungal secondary metabolites that have aroused attention due to their uncommon molecular structures and pronounced biological activities. Due to the few published studies on the ESI-MS/MS fragmentation of this important class of secondary metabolites, in the first part of our work we studied the cytochalasin D fragmentation pathways using an ESI-Q-ToF mass spectrometer coupled with liquid chromatography...
February 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28218804/detection-of-lunasin-in-quinoa-chenopodium-quinoa-willd-and-the-in-vitro-evaluation-of-its-antioxidant-and-anti-inflammatory-activities
#13
Guixing Ren, Yingying Zhu, Zhenxing Shi
BACKGROUND: Lunasin is a novel cancer preventive peptide that has been detected in various plants. However, the presence and bioactivity of lunasin in quinoa has not been demonstrated. RESULTS: Lunasin was detected in quinoa using UPLC-ESI-MS. The content in fifteen quinoa samples ranged from 1.01 × 10(-3) g kg(-1) dry seed to 4.89 × 10(-3) g kg(-1) dry seed. Significant differences (p<0.05) in lunasin content among different cultivars (yy22, xsg86, xsg59, yy30 and yy19) from the same area and the same cultivar from different areas (Hainan, Hebei and Shanxi provinces) were observed...
February 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28218654/caffeoylquinic-acids-from-the-aerial-parts-of-chrysanthemum-coronarium-l
#14
Chunpeng Wan, Shanshan Li, Lin Liu, Chuying Chen, Shuying Fan
To elucidate the chemical compositions of the aerial parts of Chrysanthemum coronarium L., the ethanol extracts of Ch. coronarium L. were firstly isolated by the MCI-gel resin column. The caffeoylquinic acid-rich fractions were further purified by various chromatographic columns including silica gel, Sephadex LH-20, and semi-preparative HPLC to yield the compounds. The purified compounds were characterized by ¹H-Nuclear Magnetic Resonance (¹H-NMR), (13)C-NMR, and high resolution electrospray ionisation mass spectral (HR-ESI-MS) spectroscopy...
February 17, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28216903/rapid-analysis-of-components-in-coptis-chinensis-franch-by-ultra-performance-liquid-chromatography-with-quadrupole-time-of-flight-mass-spectrometry
#15
Peng-Peng Tian, Xiao-Xu Zhang, Hong-Ping Wang, Pu-Ling Li, Yu-Xin Liu, Shao-Jing Li
BACKGROUND: Coptis chinensis Franch is a traditional Chinese medical herb. OBJECTIVE: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. MATERIALS AND METHODS: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216884/chemical-components-from-aloe-and-their-inhibition-of-indoleamine-2-3-dioxygenase
#16
Ya Nan Sun, Lin Ying Li, Wei Li, Jong Seong Kang, Inkyu Hwang, Young Ho Kim
BACKGROUND: In Korea, Aloe is routinely ingested as a traditional medicine or as a component of health beverages. OBJECTIVE: To research the inhibition of indoleamine 2, 3-dioxygenase (IDO) activities of components from Aloe. MATERIALS AND METHODS: the compounds were isolated by a combination of silica gel and YMC Rp-18 column chromatography, and their structures were identified by analysis of spectroscopic data (1D, 2D-NMR, and MS). All of the isolated compounds were examined for their ability to inhibit IDO, which actively suppresses immune functions by catalyzing the rate limiting reaction in the conversion of tryptophan to kynurenine...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216880/isolation-and-characterisation-of-a-proanthocyanidin-with-antioxidative-antibacterial-and-anti-cancer-properties-from-fern-blechnum-orientale
#17
How-Yee Lai, Yau-Yan Lim, Kah-Hwi Kim
BACKGROUND: Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicity against HT29 colon cancer cells by aqueous extract of B. orientale. OBJECTIVE: In this study, we attempted to isolate and identify the active compound from the aqueous extract of B...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216156/exchange-proteins-directly-activated-by-camp-induce-the-proliferation-of-rat-anterior-pituitary-gh3-cells-via-the-activation-of-extracellular-signal-regulated-kinase
#18
Wei Sun, Wei Jiao, Yimin Huang, Ran Li, Zhuo Zhang, Junwen Wang, Ting Lei
Cyclic adenosine 3'-5'-monophosphate (cAMP) plays a crucial role in regulating pituitary cell proliferation and hormone synthesis. Recent evidence suggests that exchange proteins directly activated by cAMP (Epacs) may mediate the effects of cAMP. Here we used rat anterior pituitary GH3 cells as the experiment model to demonstrate that forskolin increased the proliferation of GH3 cells and the phosphorylation of ERK1/2, and these effects were inhibited by PKA or Epac inhibitors. Epac activator 8-pCPT-2'-O-Me-cAMP increased GH3 cell proliferation and this was blocked by ESI-09, an Epac inhibitor...
February 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28215784/comparison-of-derivatization-ionization-techniques-for-liquid-chromatography-tandem-mass-spectrometry-analysis-of-oxylipins
#19
Sven W Meckelmann, Stefan Hellhake, Maryvonne Steuck, Michael Krohn, Nils Helge Schebb
The performance of two derivatization and ionization techniques for the quantitative reversed phase liquid chromatography (LC)- mass spectrometry (MS) analysis of hydroxy fatty acids (OH-PUFA) in plasma was evaluated: One used AMPP (N-(4-aminomethylphenyl)pyridinium chloride) leading to a positive charged amid-derivate which can be detected by electrospray ionization (ESI)-MS. Second yielded penta fluorobenzyl bromide (PFB) ester derivates allowing detection in electron capture atmospheric pressure chemical ionization (ecAPCI)-MS...
February 16, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/28215419/comparative-metabolite-and-genome-analysis-of-tuber-bearing-potato-species
#20
Riccardo Aversano, Felice Contaldi, Maria Grazia Adelfi, Vincenzo D'Amelia, Gianfranco Diretto, Nunziatina De Tommasi, Carmen Vaccaro, Antonio Vassallo, Domenico Carputo
The cultivated potato Solanum tuberosum is unrivalled among crop plants for its wild relatives, which potentially represent an important source of genetic diversity to improve the nutritional value of potato varieties and understand metabolism regulation. The main aim of this research was to profile human health-related metabolites in a number of clones from 13 Solanum species. Results from HPLC-DAD and LC-ESI-MS analyses highlighted a high interspecific variability in the level of metabolites analysed. Ascorbic acid was confirmed to be the most abundant antioxidant in potato and chlorogenic acid the primary polyphenol...
February 16, 2017: Phytochemistry
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