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https://www.readbyqxmd.com/read/27921344/uhplc-ms-based-hdac-assay-applied-to-bio-guided-microfractionation-of-fungal-extracts
#1
Vincent Zwick, Pierre-Marie Allard, Lucie Ory, Claudia A Simões-Pires, Laurence Marcourt, Katia Gindro, Jean-Luc Wolfender, Muriel Cuendet
INTRODUCTION: Histone deacetylases (HDAC) are considered as promising targets for cancer treatment. Today, four HDAC inhibitors, vorinostat, romidepsin, belinostat, and panobinostat, have been approved by the Food and Drug Administration (FDA) for cancer treatment, while others are in clinical trials. Among them, several are naturally occurring fungal metabolites. OBJECTIVE: To develop and optimise an enzyme assay for bio-guided identification of HDAC inhibitors in fungal strains...
December 5, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27919665/three-dimensional-visualization-of-membrane-phospholipid-distributions-in-arabidopsis-thaliana-seeds-a-spatial-perspective-of-molecular-heterogeneity
#2
Drew Sturtevant, Maria Emilia Dueñas, Young-Jin Lee, Kent D Chapman
Arabidopsis thaliana has been widely used as a model plant to study acyl lipid metabolism. Seeds of A. thaliana are quite small (approximately 500×300μm and weigh ~20μg), making lipid compositional analyses of single seeds difficult to achieve. Here we have used matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to map and visualize the three-dimensional spatial distributions of two common membrane phospholipid classes, phosphatidylcholine (PC) and phosphatidylinositol (PI), in single A...
December 2, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27917615/design-synthesis-and-evaluation-of-2-9-bis-substituted-aminomethyl-phenyl-1-10-phenanthroline-derivatives-as-g-quadruplex-ligands
#3
Nassima Meriem Gueddouda, Miyanou Rosales Hurtado, Stéphane Moreau, Luisa Ronga, Rabindra Nath Das, Solène Savrimoutou, Sandra Rubio, Adrien Marchand, Oscar Mendoza, Mathieu Marchivie, Lilian Elmi, Albain Chansavang, Vanessa Desplat, Valérie Gabelica, Anne Bourdoncle, Jean-Louis Mergny, Jean Guillon
Genomic sequences able to form guanine quadruplexes (G4) are found in oncogene promoters, in telomeres and in 5' and 3' untranslated regions as well as introns of messenger RNAs. These regions are potential targets for drugs designed to treat cancer. In the present work, we present the design and syntheses of ten new phenantroline derivatives and characterization of their interactions with G4-forming oligonucleotides. We evaluated ligand-induced stabilization and specificity and selectivity of ligands for various G4 conformations using FRET-melting experiments...
December 4, 2016: ChemMedChem
https://www.readbyqxmd.com/read/27916136/a-high-throughput-screening-method-of-bisphenols-bisphenols-digycidyl-ethers-and-their-derivatives-in-dairy-products-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#4
Yan Cheng, Xue-Mei Nie, Han-Qiu Wu, Yun-He Hong, Bing-Cheng Yang, Tong Liu, Dan Zhao, Jian-Feng Wang, Gui-Hong Yao, Feng Zhang
A simple and universal analytical method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for high throughput screening of 21 bisphenols, bisphenols digycidyl ethers and their derivatives in dairy products was developed. Response Surface Methodology (RSM) was used to optimize sample preparation conditions based on a quick, easy, cheap, effective, rugged and safe (QuEChERS) method. The analytes were extracted by using 15 mL acetonitrile with 1% acetic acid, and the extracts were further purified by using 190 mg of C18 and 390 mg of PSA...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27916133/the-use-of-a-restricted-access-volatile-supramolecular-solvent-for-the-lc-ms-ms-assay-of-bisphenol-a-in-urine-with-a-significant-reduction-of-phospholipid-based-matrix-effects
#5
José Ángel Salatti-Dorado, Noelia Caballero-Casero, María Dolores Sicilia, María Loreto Lunar, Soledad Rubio
Restricted access-volatile supramolecular solvents (RAM-VOL-SUPRAS) are here proposed as a new strategy for the quick removal of protein and phospholipids and efficient analyte extraction in LC-MS bioanalysis. Quantification of bisphenol A in urine was selected to prove the suitability of this approach for the intended purpose. RAM-VOL-SUPRAS were spontaneously synthesized in urine by addition of hexanol (83 μL) dissolved in THF (150 μL). SUPRAS composition was environment-dependent and an equation for prediction of SUPRAS volume under given experimental conditions was proposed...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915137/parental-health-shocks-and-schooling-the-impact-of-mutual-health-insurance-in-rwanda
#6
Maame Esi Woode
The goal of this study was to look at the educational spill-over effects of health insurance on schooling with a focus on the Rwandan Community Based Health Insurance Programme, the Mutual Health Insurance scheme. Using a two-person general equilibrium overlapping generations model, this paper theoretically analyses the possible effect of health insurance on the relationship between parental health shocks and child schooling. Individuals choose whether or not they want to incur a medical cost by seeking care in order to reduce the effect of health shocks on their labour market availability and productivity...
November 17, 2016: Social Science & Medicine
https://www.readbyqxmd.com/read/27915069/systematic-review-of-the-efficacy-of-particulate-vs-non-particulate-corticosteroids-in-epidural-injections
#7
REVIEW
Priyesh Mehta, Isaac Syrop, Jaspal Ricky Singh, Jonathan Kirschner
OBJECTIVE: To systematically analyze published studies in regards to the comparative efficacy of particulate versus non-particulate corticosteroids for cervical and lumbosacral epidural steroid injections (ESI) in reducing pain and improving function. TYPE: Systematic Review LITERATURE SURVEY: MEDLINE (Ovid), EMBASE, and Cochrane databases were searched from the period of 1950 to December 2015. METHODOLOGY: Criteria for inclusion in this review were: 1) Randomized controlled trials and 2) Retrospective studies comparing particulate versus non-particulate medication in fluoroscopically guided injections using a transforaminal (TF) or interlaminar (IL) approach...
November 30, 2016: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/27915054/curdione-attenuates-thrombin-induced-human-platelet-activation-%C3%AE-1-tubulin-as-a-potential-therapeutic-target
#8
Dongling Zhang, Wenhao Qiao, Yingli Zhao, Hui Fang, Dujuan Xu, Quan Xia
Rhizoma Curcumae, the dry rhizomes derived from Curcuma aromatica Salisb., are a classical Chinese medicinal herb used to activate blood circulation, remove blood stasis and alleviate pain. Our previous study proved that curdione, a sesquiterpene compound isolated from the essential oil of Curcuma aromatica Salisb. can inhibit platelet activation suggesting its significant anticoagulant and antithrombotic effects. However, the underlying mechanism of curdione mediated anti-platelet effect has not been fully elucidated...
November 30, 2016: Fitoterapia
https://www.readbyqxmd.com/read/27914321/functional-regulation-of-ginsenoside-biosynthesis-by-rna-interferences-of-a-udp-glycosyltransferase-gene-in-panax-ginseng-and-panax-quinquefolius
#9
Chao Lu, Shoujing Zhao, Guanning Wei, Huijuan Zhao, Qingling Qu
Panax ginseng (Asian ginseng) and Panax quinquefolius (American ginseng) have been used as medicinal and functional herbal remedies worldwide. Different properties of P. ginseng and P. quinquefolius were confirmed not only in clinical findings, but also at cellular and molecular levels. The major pharmacological ingredients of P. ginseng and P. quinquefolius are the triterpene saponins known as ginsenosides. The P. ginseng roots contain a higher ratio of ginsenoside Rg1:Rb1 than that in P. quinquefolius...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27914034/identification-of-oxidized-phosphatidylinositols-present-in-oxldl-and-human-atherosclerotic-plaque
#10
Devin Hasanally, Andrea Edel, Rakesh Chaudhary, Amir Ravandi
Oxidized low-density lipoprotein (OxLDL) plays an important role in initiation and progression of atherosclerosis. Proatherogenic effects of OxLDL have been attributed to bioactive phospholipids generated during LDL oxidation. It is unknown what effect oxidation has on the phosphatidylinositol (PtdIns) molecules in LDL, even though PtdIns is 6% of the total LDL phospholipid pool. We sought to identify and quantitate oxidized phosphatidylinositol (OxPtdIns) species in OxLDL and human atherosclerotic plaque. Bovine liver PtdIns was subjected to non-enzymatic and lipoxygenase-catalyzed oxidation...
December 2, 2016: Lipids
https://www.readbyqxmd.com/read/27913986/establishing-clinical-meaning-and-defining-important-differences-for-patient-reported-outcomes-measurement-information-system-promis-%C3%A2-measures-in-juvenile-idiopathic-arthritis-using-standard-setting-with-patients-parents-and-providers
#11
Esi M Morgan, Constance A Mara, Bin Huang, Kimberly Barnett, Adam C Carle, Jennifer E Farrell, Karon F Cook
BACKGROUND: Patient-Reported Outcomes Measurement Information System (PROMIS) measures are used increasingly in clinical care. However, for juvenile idiopathic arthritis (JIA), scores lack a framework for interpretation of clinical severity, and minimally important differences (MID) have not been established, which are necessary to evaluate the importance of change. METHODS: We identified clinical severity thresholds for pediatric PROMIS measures of mobility, upper extremity function (UE), fatigue, and pain interference working with adolescents with JIA, parents of JIA patients, and clinicians, using a standard setting methodology modified from educational testing...
December 2, 2016: Quality of Life Research
https://www.readbyqxmd.com/read/27912969/triterpenoid-saponins-with-anti-inflammatory-activities-from-ilex-pubescens-roots
#12
Peng Wu, Hui Gao, Jian-Xin Liu, Liang Liu, Hua Zhou, Zhong-Qiu Liu
Seven triterpenoid saponins, named ilexsaponin I-O, along with twelve known ones, were isolated from the roots of Ilex pubescens. The structures of all compounds were elucidated by use of extensive spectroscopic methods (IR, HR-ESI-MS, and 1D and 2D NMR). Sugar residues obtained after acid hydrolysis were identified by TLC and HPLC. The in vitro anti-inflammatory effects of the triterpenoid saponins were also evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Among the isolated saponins, seven compounds were shown to inhibit LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production by suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively, in LPS-stimulated RAW 264...
November 29, 2016: Phytochemistry
https://www.readbyqxmd.com/read/27910788/metabolomic-investigation-of-rat-serum-following-oral-administration-of-the-willow-bracket-medicinal-mushroom-phellinus-igniarius-agaricomycetes-by-uplc-hdms
#13
Yu Dong, Ying He, Zhongming Yu, Yang Zhang, Nani Wang, Dan Shou, Changyu Li
The medicinal willow bracket mushroom, Phellinus igniarius, is a species that has been reported to possess antibacterial, antioxidative, antitumor, antidiabetic, and antihyperlipidemia activities. The aim of this study was to elucidate the changes in endogenous metabolites after oral administration of a decoction of Ph. Igniarius. Ultraperformance liquid chromatography (UPLC)/electrospray ionization synapt high-definition mass spectrometry (ESI-HDMS) combined with pattern recognition approaches, including principal component analysis and orthogonal partial least squares discriminant analysis, were integrated to discover differentiating metabolites...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910158/hptlc-bioautography-guided-isolation-of-%C3%AE-glucosidase-inhibiting-compounds-from-justicia-secunda-vahl-acanthaceae
#14
Barbara A Theiler, Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O Espinoza Caisa, Sabine Glasl
INTRODUCTION: α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from the leaves of Justicia secunda Vahl are used to treat diabetes mellitus symptoms. OBJECTIVE: To screen for α-glucosidase inhibitors in J. secunda via HPTLC bioautography...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27909974/native-mass-spectrometry-what-is-in-the-name
#15
Aneika C Leney, Albert J R Heck
Electrospray ionization mass spectrometry (ESI-MS) is nowadays one of the cornerstones of biomolecular mass spectrometry and proteomics. Advances in sample preparation and mass analyzers have enabled researchers to extract much more information from biological samples than just the molecular weight. In particular, relevant for structural biology, noncovalent protein-protein and protein-ligand complexes can now also be analyzed by MS. For these types of analyses, assemblies need to be retained in their native quaternary state in the gas phase...
December 1, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27909427/biocombinatorial-synthesis-of-novel-lipopeptides-by-com-domain-mediated-reprogramming-of-the-plipastatin-nrps-complex
#16
Hongxia Liu, Ling Gao, Jinzhi Han, Zhi Ma, Zhaoxin Lu, Chen Dai, Chong Zhang, Xiaomei Bie
Both donors and acceptors of communication-mediating (COM) domains are essential for coordinating intermolecular communication within nonribosomal peptides synthetases (NRPSs) complexes. Different sets of COM domains provide selectivity, allowing NRPSs to utilize different natural biosynthetic templates. In this study, novel lipopeptides were synthesized by reprogramming the plipastatin biosynthetic machinery. A Thr-to-Asp point mutation was sufficient to shift the selectivity of the donor COM domain of ppsB toward that of ppsD...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27908498/hydrophilic-interaction-and-reversed-phase-mixed-mode-liquid-chromatography-coupled-to-high-resolution-tandem-mass-spectrometry-for-polar-lipids-analysis
#17
Sara Granafei, Pietro Azzone, Vito Alessandro Spinelli, Ilario Losito, Francesco Palmisano, Tommaso R I Cataldi
A hydrophilic interaction liquid chromatography (HILIC) fused-core column (150×2.1mm ID, 2.7μm particle size) and a short reversed-phase liquid chromatography (RPLC) column (20mm×2.1mm ID, 1.9μm) were serially coupled to perform mixed-mode chromatography (MMC) on complex mixtures of phospholipids (PL). Mobile phase composition and gradient elution program were, preliminarily, optimized using a mixture of phosphatidylcholines (PC), phosphatidylethanolamines (PE), their corresponding lyso-forms (LPC and LPE), and sphingomyelins (SM)...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27908495/analysis-of-low-abundant-trehalose-6-phosphate-and-related-metabolites-in-medicago-truncatula-by-hydrophilic-interaction-liquid-chromatography-triple-quadrupole-mass-spectrometry
#18
Ana Teresa Mata, Tiago Filipe Jorge, João Ferreira, Maria do Rosário Bronze, Diana Branco, Pedro Fevereiro, Susana Araújo, Carla António
Trehalose-6-phosphate (T6P) is an important signaling metabolite involved in plant growth control that inhibits the sucrose nonfermenting-1-related protein kinase 1 (SnRK1), a key regulator of energy and carbon metabolism in plants. The quantification of T6P in plant tissues is fundamental to improve our understanding of sugar signaling and the links between plant growth and development in response to stress conditions. However, the almost undetectable levels of T6P together with the complex plant matrix and the presence of T6P isomers such as sucrose-6-phosphate (S6P), makes the detection of this metabolite challenging...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27907865/biophysical-characterization-and-antineoplastic-activity-of-new-bis-thiosemicarbazonato-cu-ii-complexes
#19
Elisa Palma, Filipa Mendes, Goreti Ribeiro Morais, Inês Rodrigues, Isabel Cordeiro Santos, Maria Paula C Campello, Paula Raposinho, Isabel Correia, Sofia Gama, Dulce Belo, Vítor Alves, Antero J Abrunhosa, Isabel Santos, António Paulo
Aiming to explore alternative mechanisms of cellular uptake and cytotoxicity, we have studied a new family of copper(II) complexes (CuL(1)-CuL(4)) with bis(thiosemicarbazone) (BTSC) ligands containing pendant protonable cyclic amines (morpholine and piperidine). Herein, we report on the synthesis and characterization of these new complexes, as well as on their biological performance (cytotoxic activity, cellular uptake, protein and DNA binding), in comparison with the parental Cu(II)ATSM (ATSM=diacetyl-bis(N4-methylthiosemicarbazonate) complex without pendant cyclic amines...
November 23, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27907110/electrospray-ionization-efficiency-is-dependent-on-different-molecular-descriptors-with-respect-to-solvent-ph-and-instrumental-configuration
#20
Andreas Kiontke, Ariana Oliveira-Birkmeier, Andreas Opitz, Claudia Birkemeyer
Over the past decades, electrospray ionization for mass spectrometry (ESI-MS) has become one of the most commonly employed techniques in analytical chemistry, mainly due to its broad applicability to polar and semipolar compounds and the superior selectivity which is achieved in combination with high resolution separation techniques. However, responsiveness of an analytical method also determines its suitability for the quantitation of chemical compounds; and in electrospray ionization for mass spectrometry, it can vary significantly among different analytes with identical solution concentrations...
2016: PloS One
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