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https://www.readbyqxmd.com/read/28446017/dexmedetomidine-oral-mucosa-patch-for-sedation-suppresses-apoptosis-in-hippocampus-of-normal-rats
#1
Je Hoon Park, Il Gyu Ko, Sung Eun Kim, Jun Jang Jin, Lakkyong Hwang, Chang Ju Kim, Soo Hwan Yoon, Jongki Hong, Jun Young Chung, Deok Won Lee
PURPOSE: Dexmedetomidine, an α2-adrenergic agonist, provides sedative and analgesic effects without significant respiratory depression. Dexmedetomidine has been suggested to have an antiapoptotic effect in response to various brain insults. We developed an oral mucosa patch using dexmedetomidine for sedation. The effects of the dexmedetomidine oral mucosa patch on cell proliferation and apoptosis in the hippocampus were evaluated. METHODS: A hydrogel oral mucosa patch was adhered onto the oral cavity of physiologically normal rats, and was attached for 2 hours, 6 hours, 12 hours, or 24 hours...
April 2017: International Neurourology Journal
https://www.readbyqxmd.com/read/28445851/development-and-validation-of-a-uhplc-esi-ms-ms-method-for-the-simultaneous-quantification-of-mammal-lysophosphatidylcholines-and-lysophosphatidylethanolamines-in-serum
#2
Susana Suárez-García, Lluís Arola, Aïda Pascual-Serrano, Anna Arola-Arnal, Gerard Aragonès, Cinta Bladé, Manuel Suárez
Recent investigations based on non-targeted metabolomics have proposed lysophospholipids (Lyso-PLs) as biomarkers of different diseases. In particular, lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) have been associated with serious lipid pathologies. Methods to determine the different molecular species in a biological sample and to quantify even less abundant species are required for the evaluation of the Lyso-PL pattern as a novel comprehensive biomarker of dyslipidemia. This study describes the development and validation of an ultra-high-performance liquid chromatography coupled to tandem mass spectrometry assay for the determination of a large number of Lyso-PCs and Lyso-PEs in biological samples...
April 18, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445846/a-sharp-robust-and-quantitative-method-by-liquid-chromatography-tandem-mass-spectrometry-for-the-measurement-of-ead-for-acute-radiation-syndrome-and-its-application
#3
Yiwei Zhang, Jian Li, Zhiyun Meng, Xiaoxia Zhu, Hui Gan, Ruolan Gu, Zhuona Wu, Ying Zheng, Jinbin Wei, Guifang Dou
17-Ethinyl-3,17-dihydroxyandrost-5-ene (EAD) is an agent designed for the treatment of acute radiation syndrome (ARS). Given its vital role played in the prevention and mitigation of ARS, the development of a sharp, sensitive and robust liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor the metabolism of EAD in vivo was crucial. A new method was constructed and validated for the determination of EAD with the internal standard of androst-5-ene-3β,17β-diol (5-AED). The blood samples were precipitated with methanol, centrifuged, from which the supernatant was separated on UPLC with C18 column and eluted in gradient with acetonitrile and Milli-Q water both containing 0...
April 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445428/residue-modification-and-mass-spectrometry-for-the-investigation-of-structural-and-metalation-properties-of-metallothionein-and-cysteine-rich-proteins
#4
REVIEW
Gordon W Irvine, Martin J Stillman
Structural information regarding metallothioneins (MTs) has been hard to come by due to its highly dynamic nature in the absence of metal-thiolate cluster formation and crystallization difficulties. Thus, typical spectroscopic methods for structural determination are limited in their usefulness when applied to MTs. Mass spectrometric methods have revolutionized our understanding of protein dynamics, structure, and folding. Recently, advances have been made in residue modification mass spectrometry in order to probe the hard-to-characterize structure of apo- and partially metalated MTs...
April 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28445422/bioactive-components-from-qingwen-baidu-decoction-against-lps-induced-acute-lung-injury-in-rats
#5
Qi Zhang, Hai-Min Lei, Peng-Long Wang, Zhi-Qiang Ma, Yan Zhang, Jing-Jing Wu, Jing Nie, Su-Juan Chen, Wen-Jie Han, Qing Wang, Dan-Yang Chen, Cheng-Ke Cai, Qiang Li
Qingwen Baidu Decoction (QBD) is an extraordinarily "cold" formula. It was traditionally used to cure epidemic hemorrhagic fever, intestinal typhoid fever, influenza, sepsis and so on. The purpose of this study was to discover relationships between the change of the constituents in different extracts of QBD and the pharmacological effect in a rat model of acute lung injury (ALI) induced by lipopolysaccharide (LPS). The study aimed to discover the changes in constituents of different QBD extracts and the pharmacological effects on acute lung injury (ALI) induced by LPS...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28445407/application-of-uhplc-esi-q-tof-ms-to-identify-multiple-constituents-in-processed-products-of-the-herbal-medicine-ligustri-lucidi-fructus
#6
Hui Li, Weifeng Yao, Qinan Liu, Jia Xu, Beihua Bao, Mingqiu Shan, Yudan Cao, Fangfang Cheng, Anwei Ding, Li Zhang
Ligustri Lucidi Fructus (LLF), the fruit of Ligustrum lucidum Ait. (Oleaceae), has been used as a common herbal medicine in clinical practice in China for nearly 2000 years. In most cases, LLF is prescribed in decoctions in the form of processed products rather than crude drugs. In this study, an ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UHPLC-ESI-Q-TOF-MS) method was established for rapid separation and identification of multiple constituents in the 80% methanol extract of processed-LLF...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444903/electrospray-ionization-mass-spectrometry-for-the-hydrolysis-complexes-of-cisplatin-implications-for-the-hydrolysis-process-of-platinum-complexes
#7
Feifan Xie, Pieter Colin, Jan Van Bocxlaer
Non-enzyme dependent hydrolysis of the drug cisplatin is important for its mode of action and toxicity. However, up until today, the hydrolysis process of cisplatin is still not completely understood. In the present study, the hydrolysis of cisplatin in an aqueous solution was systematically investigated using electrospray ionization mass spectrometry coupled to liquid chromatography. A variety of previously unreported hydrolysis complexes corresponding to mono-, di-, and trimeric species were detected and identified...
April 26, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28443078/phytochemical-characterization-of-terminalia-catappa-linn-extracts-and-their-antifungal-activities-against-candida-spp
#8
Analucia G Terças, Andrea de Souza Monteiro, Eduardo B Moffa, Julliana R A Dos Santos, Eduardo M de Sousa, Anna R B Pinto, Paola C da Silva Costa, Antonio C R Borges, Luce M B Torres, Allan K D Barros Filho, Elizabeth S Fernandes, Cristina de Andrade Monteiro
Terminalia catappa Linn bark is used to treat dysentery by various populations in Southeast Asian countries, and its leaves have also been used in traditional medicine to treat hepatitis in India and the Philippines. Here, the antifungal actions of crude hydro-alcoholic extract (TcHE) and fractions from T. catappa leaves were assessed via the agar diffusion and microdilution tests on Candida reference strains and clinical isolates from patients with acquired immunodeficiency syndrome (AIDS). Additionally, the potential cytotoxic effects of TcHE were assessed on cultured human peripheral blood mononuclear cells (PBMC)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28441878/four-new-anthraquinones-from-a-soil-actinomycete-streptomyces-sp-ws-13394-and-their-bioactivities
#9
Zhaoyuan Wu, Yani Zhang, Wei Fang, Liqiao Shi, Zhongyi Wan
Further chemical study of secondary metabolites from the soil actinomycete Streptomyces sp. WS-13394 resulted in the isolation of four new alkylated anthraquinone analogues (5-8). Their structures were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, 1D and 2D NMR. The new compounds, together with analogues obtained before (1-4), were tested for their in vitro cytotoxicity against Huh-7 and SGC-7901.
April 25, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28440576/new-cascarosides-from-rhamnus-purshiana-and-fragmentation-studies-of-the-class-by-it-ms
#10
Daniel P Demarque, Danielle R Pinho, Daniel R Callejon, Gibson G de Oliveira, Denise B Silva, Carlos A Carollo, Norberto P Lopes
RATIONALE: Anthrone and oxanthrone are important anthraquinone derivatives present in medicinal plants and are used in therapeutics as laxatives. Some of these plants need to be stored at least one year before they can be used in order to oxidize anthrones into oxanthrones, so to avoid severe diarrhea and dehydration. Therefore, this work aimed to characterize fragmentation reactions between these anthraquinones to provide an easy way to differentiate between the two classes, since it is necessary and important to discriminate and identify these derivatives in laxative plants and phytotherapic drugs...
April 25, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28440570/mass-spectrometric-characterization-of-the-selective-androgen-receptor-modulator-sarm-yk-11-for-doping-control-purposes
#11
Mario Thevis, Thomas Piper, Josef Dib, Andreas Lagojda, Dirk Kuehne, Lars Packschies, Hans Geyer, Wilhelm Schaenzer
RATIONALE: Selective androgen receptor modulators (SARMs) represent an emerging class of therapeutics targeting inter alia conditions referred to as cachexia and sarcopenia. Due to their anabolic properties, the use of SARMs is prohibited in sports as regulated by the World Anti-Doping Agency (WADA), and doping control laboratories test for these anabolic agents in blood and urine. In order to accomplish and maintain comprehensive test methods, the characterization of new drug candidates is critical for efficient sports drug testing...
April 25, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28440064/synthetic-and-analytical-strategies-for-the-quantification-of-phenyl-%C3%AE-valerolactone-conjugated-metabolites-in-human-urine
#12
Nicoletta Brindani, Pedro Mena, Luca Calani, Iris Benzie, Siu-Wai Choi, Furio Brighenti, Franca Zanardi, Claudio Curti, Daniele Del Rio
SCOPE: The contribution of the gut microbiota to the metabolism of catechins and proanthocyanidins remains unclear. Phenyl-γ-valerolactones have been identified as the most representative metabolites of these dietary flavan-3-ols, but their accurate quantification has posed problems because of a lack of appropriate bioanalytical standards. This work aimed at synthesizing a novel set of sulphate- and glucuronide-conjugated phenyl-γ-valerolactones and at developing an analytical platform using UHPLC-ESI-MS/MS for their quantification in urine...
April 25, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28437805/anti-inflammatory-antipyretic-and-antinociceptive-effects-of-a-cressa-cretica-aqueous-extract
#13
Heba Mohammed Ibrahim Abdallah, Abdelsamed Ibrahim Elshamy, Abd El-Nasser Gaber El Gendy, Ahmed Mohamed Abd El-Gawad, Elsayed Abouelfotowh Omer, Marinella De Leo, Luisa Pistelli
Cressa cretica is a widely grown halophytic plant traditionally used for the treatment of different ailments. Previous investigations reported its biological activity on a wide spectrum of diseases. In this study, in vivo antinociceptive, anti-inflammatory, and antipyretic activities of C. cretica aqueous extract whole plant were evaluated. In addition, the total polyphenol content, the total flavonoid content, and the chemical characterization of the extract were performed. C. cretica showed writhing inhibition in acetic acid-induced peripheral nociception of 43 and 48 % at doses of 50 and 100 mg/kg, respectively...
April 24, 2017: Planta Medica
https://www.readbyqxmd.com/read/28436866/universal-multimode-background-subtraction
#14
Hasan Sajid, Sen-Ching Samson Cheung
In this paper, we present a complete change detection system named Multimode Background Subtraction (MBS). The universal nature of system allows it to robustly handle multitude of challenges associated with video change detection such as illumination changes, dynamic background, camera jitter, moving camera etc. The system comprises of multiple innovative mechanisms in background modeling, model update, pixel classification and the use of multiple color spaces. The system first creates multiple background models of the scene followed by an initial foreground/background probability estimation for each pixel...
April 19, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28436051/a-novel-mass-spectrometry-based-method-for-simultaneous-determination-of-asymmetric-and-symmetric-dimethylarginine-l-arginine-and-l-citrulline-optimized-for-lc-ms-tof-and-lc-ms-ms
#15
Jerzy Wiśniewski, Mariusz G Fleszar, Joanna Piechowicz, Małgorzata Krzystek-Korpacka, Angelika Chachaj, Andrzej Szuba, Katarzyna Lorenc-Kukula, Leszek Masłowski, Wojciech Witkiewicz, Andrzej Gamian
Nitric oxide (NO) is a regulatory molecule involved in many biological processes. NO is produced by nitric oxide synthase by conversion of L-arginine to L-citrulline. L-arginine methylated derivatives, asymmetric and symmetric dimethylarginines (ADMA and SDMA), regulate L-arginine availability and the activity of nitric oxide synthase. As such, they have been frequently investigated as potential biomarkers in pathologies associated with dysfunctions in NO synthesis. Here, we present a new multistep analytical methodology based on liquid chromatography combined with mass spectrometry for the accurate identification of L-arginine, L-citrulline, ADMA, and SDMA...
April 24, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28434712/evidence-for-an-imidazoline-by-product-from-glycans-using-tandem-mass-spectrometry
#16
Robert J Duff, Elaine Smith, Wenzhou Li, Szilan Fodor
Herein is reported the separation and identification of a previously unknown imidazoline by-product originating from the fluorescent labeling procedure when applied to enzymatically released N-linked glycans of a human IgG1. The imidazoline by-product was generated via the reductive amination procedure with either sodium cyanoborohydride or 2-picoline borane. Using ultra performance liquid chromatography (UPLC) in conjunction with hydrophilic interaction-based chromatography (HILIC), the 2-aminobenzoic acid (2-AA)-labeled glycans were well-resolved from imidazoline by-products to facilitate direct identification utilizing electrospray ionization mass spectrometry (ESI-MS) with fragmentation...
April 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28434226/in-situ-microprobe-single-cell-capillary-electrophoresis-mass-spectrometry-metabolic-reorganization-in-single-differentiating-cells-in-the-live-vertebrate-x-laevis-embryo
#17
Rosemary M Onjiko, Erika P Portero, Sally A Moody, Peter Nemes
Knowledge of single-cell metabolism would provide a powerful window to cell activity changes as cells differentiate to all the tissues of the vertebrate embryo. However, single-cell mass spectrometry technologies have not yet been made compatible with complex three-dimensional changes and rapidly decreasing cell sizes during early development of the embryo. Here, we bridge this technological gap by integrating capillary microsampling, microscale metabolite extraction, and capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable direct metabolic metabolic analysis of identified cells in the live frog embryo (Xenopus laevis)...
April 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28433954/lipidomic-profile-in-three-species-of-dinoflagellates-amphidinium-carterae-cystodinium-sp-and-peridinium-aciculiferum-containing-very-long-chain-polyunsaturated-fatty-acids
#18
Tomáš Řezanka, Jaromír Lukavský, Linda Nedbalová, Karel Sigler
This study describes the identification of very long chain polyunsaturated fatty acids (VLCPUFAs) in three strains of dinoflagellates (Amphidinium carterae, Cystodinium sp., and Peridinium aciculiferum). The strains were cultivated and their lipidomic profiles were obtained by high resolution mass spectrometry with the aid of positive and negative electrospray ionization (ESI) mode by Orbitrap apparatus. Hydrophilic interaction liquid chromatography (HILIC/ESI) was used to separate major lipid classes of the three genera of dinoflagellates by neutral loss scan showing the ion [M + H-28:8](+), where 28:8 was octacosaoctaenoic acid, and by precursor ion scanning of ions at m/z 407, which was an ion corresponding to the structure of acyl of 28:8 acid (C27H39COO(-))...
April 20, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28433761/a-glycoproteomic-approach-reveals-that-the-s-layer-glycoprotein-of-lactobacillus-kefiri-cidca-83111-is-o-and-n-glycosylated
#19
Gustavo J Cavallero, Mariano Malamud, Adriana C Casabuono, M de Los Ángeles Serradell, Alicia S Couto
In Gram-positive bacteria, such as lactic acid bacteria, general glycosylation systems have not been documented so far. The aim of this work was to characterize in detail the glycosylation of the S-layer protein of Lactobacillus kefiri CIDCA 83111. A reductive β-elimination treatment followed by anion exchange high performance liquid chromatography analysis was useful to characterize the O-glycosidic structures. MALDI-TOF mass spectrometry analysis confirmed the presence of oligosaccharides bearing from 5 to 8 glucose units carrying galacturonic acid...
April 19, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28433747/chemical-composition-of-ocimum-sanctum-by-lc-esi-ms-ms-analysis-and-its-protective-effects-against-smoke-induced-lung-and-neuronal-tissue-damage-in-rats
#20
M P Venuprasad, Hemanth Kumar Kandikattu, Sakina Razack, Narayanappa Amruta, Farhath Khanum
Smoke induced oxidative stress is known to cause various cancers and associated health problems including lung cancer. Herbal extracts have been reported as antioxidant supplements which attenuate free radical induced oxidative damage of tissues, among which Ocimum sanctum has been reported as the elixir of life due to its innumerable health benefits. In the present study, we investigated the protective effect of O. sanctum against cracker smoke induced lung and brain tissue damage. The results of the study demonstrate that O...
April 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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