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Priti Sengupta, Aniruddha Ganguly, Adity Bose
The crucial role of chemosensor for the immediate recognition of environment pollutant motivates the researchers to develop variety of sensing protocols. Of various chemosensory protocols, the colour change observed by the naked eye is considered to be a conceivable and on-site way to indicate the presence of an analyte. We herein report a colourimetric and commercially available absorption probe, sinapic acid (SA) that is completely ready to use for "on-site" visual determination of copper ions. The molecule, SA is well-known phenolic acid, often utilized for its antibacterial activity...
March 3, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Haline G O Alvim, Jose Raimundo Correa, José A F Assumpção, Wender Alves da Silva, Marcelo Oliveira Rodrigues, Julio Lemos de Macedo, Mariana Fioramonte, Fabio Cesar Gozzo, Claudia C Gatto, Brenno A D Neto
The current manuscript describes the use of a heteropolyacid-containing task-specific ionic liquid, supported in imidazolium-based ionic liquids, as the catalyst for an efficient multicomponent synthesis of hexahydroimidazo[1,2-alpha]pyridine derivatives. The reactions conditions were fully optimized and the bridgehead nitrogen heterocycle derivatives could be obtained in just one hour exclusively as a single isomer (trans). Single crystal X-ray analysis confirmed the trans derivative as the only isomer. The mechanism of the reaction was investigated by ESI(+)-MS(/MS) and the use of the elegant charge-tag strategy allowed a catalytic cycle to be proposed for the current transformation and revealed the possibility of at least two reaction pathways...
March 16, 2018: Journal of Organic Chemistry
Zia Uddin, Yeong Hun Song, Mahboob Ullah, Zuopeng Li, Jeong Yoon Kim, Ki Hun Park
Diabetes mellitus is one of a major worldwide concerns, regulated by either defects in secretion or action of insulin, or both. Insulin signaling down-regulation has been related with over activity of protein tyrosine phosphatase 1B (PTP1B) enzyme, which has been a promising target for the treatment of diabetes mellitus. Herein, activity guided separation of methanol extract (95%) of Dodonaea viscosa aerial parts afforded nine ( 1 - 9 ) polyphenolic compounds, all of them were identified through spectroscopic data including 2D NMR and HREIMS...
2018: Frontiers in Chemistry
Holger Joswig, Armin Neff, Christina Ruppert, Gerhard Hildebrandt, Martin Nikolaus Stienen
BACKGROUND: The predictive value of short-term arm pain relief after 'indirect' cervical epidural steroid injection (ESI) for the 1-month treatment response has been previously demonstrated. It remained to be answered whether the long-term response could be estimated by the early post-interventional pain course as well. METHODS: Prospective observational study, following a cohort of n = 45 patients for a period of 24 months after 'indirect' ESI for radiculopathy secondary to a single-level cervical disk herniation (CDH)...
March 14, 2018: Acta Neurochirurgica
Gang Wang, Jing Liang, Zishan Jia, Lei Wan, Mingxia Yang
RATIONALE: Spinal cord infarction is one of the complications of epidural steroid injections (ESIs), but has only been reported in cervical vertebra by transforaminal injection and lumbar vertebra by transforaminal injection; and up to now, there is no reporting about spinal cord infarction caused by caudal injection. Here, we report a case. PATIENT CONCERNS: A 52-year-old man was admitted to our hospital. He was diagnosed as lumbar disc herniation in other hospital, and the patient suffered bilateral lower limb motor and sensory disorders after administration of caudal ESI...
March 2018: Medicine (Baltimore)
Martin Eckardt, Annemarie Greb, Thomas J Simat
Polyphenylsulfone (PPSU) is a new material for the production of baby bottles. PPSU is a polyether plastic formally composed of bisphenol S (BPS) and 4,4'-dihydroxybiphenyl (DHBP), which both have slight endocrine activities in in-vitro-tests. So far, little is known about the presence and the release of potentially hazardous substances from PPSU baby bottles. In our present study, we present a three step approach for the analysis of PPSU starting with polymer characterization in terms of chemical structure, total oligomer content and hydrolytic stability...
March 14, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
C Fraschetti, L Guarcini, C Zazza, L Mannina, S Circi, S Piccirillo, B Chiavarino, A Filippi
Conformational characterization of single α- and β-epimers of galactosamine in solution still remains an intriguing task because of their flexibility and ability to interconvert. This difficulty was circumvented by recording several "snapshots" of the epimerization process by means of fast ESI vaporization of a galactosamine·HCl sample solution at different times. Consequently, the so generated gaseous mixtures were spectroscopically investigated and the specific conformational features of both α- and β-epimers were assigned, despite the overlapping of several IR signals...
March 14, 2018: Physical Chemistry Chemical Physics: PCCP
Annika Wahl, Erik van den Akker, Lucija Klaric, Jerko Štambuk, Elisa Benedetti, Rosina Plomp, Genadij Razdorov, Irena Trbojević-Akmačić, Joris Deelen, Diana van Heemst, P Eline Slagboom, Frano Vučković, Harald Grallert, Jan Krumsiek, Konstantin Strauch, Annette Peters, Thomas Meitinger, Caroline Hayward, Manfred Wuhrer, Marian Beekman, Gordan Lauc, Christian Gieger
Immunoglobulin G (IgG), a glycoprotein secreted by plasma B-cells, plays a major role in the human adaptive immune response and are associated with a wide range of diseases. Glycosylation of the Fc binding region of IgGs, responsible for the antibody's effector function, is essential for prompting a proper immune response. This study focuses on the general genetic impact on IgG glycosylation as well as corresponding subclass specificities. To identify genetic loci involved in IgG glycosylation, we performed a genome-wide association study (GWAS) on liquid chromatography electrospray mass spectrometry (LC-ESI-MS)-measured IgG glycopeptides of 1,823 individuals in the Cooperative Health Research in the Augsburg Region (KORA F4) study cohort...
2018: Frontiers in Immunology
George Cătălin Marinescu, Roua-Gabriela Popescu, Anca Dinischiotu
Over 12% of the world's health resources are spent on treating diabetes, as high blood glucose is the third cause of mortality worldwide. Insulin resistance is the basis of the most common form of diabetes: type 2 diabetes. Recent animal studies report successful attempts at reversing type 2 diabetes by the administering of the NAD+ precursor nicotinamide mononucleotide (NMN). However, the current high price of this molecule urges for more efficient and cost-effective production methods. This work proposes a method for purifying NMN by Size Exclusion Chromatography (SEC) on silica with a covalently attached coating of poly(2-hydroxyethyl aspartamide) (PolyHEA) stationary phase using an isocratic elution with a denaturing mobile phase (50 mM formic acid) from a complex molecular mixture such as a fermentation broth...
March 13, 2018: Scientific Reports
Hyeon-Cheol Lee, Takehiko Yokomizo
Recent advances in mass spectrometry have expanded our knowledge of lipids and lipid metabolic pathways involved in many (patho)physiological events. Targeted and non-targeted lipidomics are powerful analytical strategies with distinct features, and a combination of these two approaches is often employed to maximize the coverage of lipid species detected and quantified in complex biological matrices. This review briefly summarizes the applications of targeted and non-targeted lipidomics, mainly focusing on electrospray ionization-liquid chromatography-tandem mass spectrometry (ESI-LC-MS/MS), along with recent technical advances in the field...
March 10, 2018: Biochemical and Biophysical Research Communications
J Wu, H Zhang, W Zhang, J Zhang, X Lin, G Xue, F Gao
Trefoil factor 3 (TFF3), a regulatory protein composed of 59 amino acids, has been suggested to be involved in pathogen- esis, proliferation, invasion, migration and apoptosis in multiple malignant tumors. However, the roles of TFF3 concerning the viability, migration and invasion in papillary thyroid carcinoma cells have not yet been studied. This study aimed to investigate the effect of TFF3 knockdown on a thyroid papillary carcinoma TPC-1 cell line both in vitro and in vivo. In the present study, lentivirus-mediated short hairpin RNA (shRNA) targeting TFF3 plasmids were first constructed and stable TPC-1 cells were obtained while their TFF3 gene was silenced with either shTFF3-TPC-1, or a scrambled shRNA control...
2018: Neoplasma
Syed Qaiser Shah, Gul-E-Raana, Ghias Uddin
This investigation aimed to modify finasteride (1) to finasteride dithiocarbamate (2) for subsequent synthesis of the rhenium analogue (3) and [99m Tc]tricarbonyl complexes (4), to assess its prostate cancer (PCa) targeting potential in a rat model. To validate the identity of (4), reference (3) has been synthesized using fac-[Net4 ]2 [ReBr3 (CO)3 ] precursor and characterized by1 H-NMR,13 C-NMR , ESI-MS and elemental analysis. The analogue (4) was synthesized using fac-[99m Tc(H2 O)3 (CO)3 ]+ precursor and its structure was confirmed by comparative HPLC using (3) as a reference...
March 13, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
Anastasia A Andrianova, Thomas DiProspero, Clayton Geib, Irina P Smoliakova, Evguenii I Kozliak, Alena Kubátová
The capability to characterize lignin, lignocellulose, and their degradation products is essential for the development of new renewable feedstocks. Electrospray ionization high-resolution time-of-flight mass spectrometry (ESI-HR TOF-MS) method was developed expanding the lignomics toolkit while targeting the simultaneous detection of low and high molecular weight (MW) lignin species. The effect of a broad range of electrolytes and various ionization conditions on ion formation and ionization effectiveness was studied using a suite of mono-, di-, and triarene lignin model compounds as well as kraft alkali lignin...
March 12, 2018: Journal of the American Society for Mass Spectrometry
R Andler, S Hiessl, O Yücel, M Tesch, A Steinbüchel
Potential biotechnological recycling processes for rubber products include the bacterial degradation of poly(cis-1,4-isoprene) (IR) in order to achieve its total biodegradation or its biotransformation into useful products. The actinomycete Gordonia polyisoprenivorans strain VH2 catalyzes the degradation of IR and enables its use as a sole carbon source via β-oxidation. The initial cleavage reaction is catalyzed by the extracellular latex clearing protein (Lcp). This dioxygenase is the key enzyme for the formation of oligo(cis-1,4-isoprene) molecules with different lengths, i...
March 9, 2018: New Biotechnology
Yongqiang Liu, Melissa Siard, Amanda Adams, Michael L Keowen, Travis K Miller, Frank Garza, Frank M Andrews, Navindra P Seeram
The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI-MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma...
March 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Nicola Salvarese, Barbara Spolaore, Selena Marangoni, Anna Pasin, Alessandro Galenda, Sergio Tamburini, Gianfranco Cicoria, Fiorenzo Refosco, Cristina Bolzati
An assessment study involving the use of the transglutaminase (TGase) conjugation method and the nitride-technetium-99m labelling on a bis(thiosemicarbazone) (BTS) bifunctional chelating agent is presented. The previously described chelator diacetyl-2-(N4 -methyl-3-thiosemicarbazone)-3-(N4 -amino-3-thiosemicarbazone), H2 ATSM/A, has been functionalized with 6-aminohexanoic acid (ε-Ahx) to generate the bifunctional chelating agent diacetyl-2-(N4 -methyl-3-thiosemicarbazone)-3-[N4 -(amino)-(6-aminohexanoic acid)-3-thiosemicarbazone], H2 ATSM/A-ε-Ahx (1), suitable for conjugation to glutamine (Gln) residues of bioactive molecules via TGase...
March 2, 2018: Journal of Inorganic Biochemistry
Nagamalai Vasimalai, Vânia Vilas-Boas, Juan Gallo, María de Fátima Cerqueira, Mario Menéndez-Miranda, José Manuel Costa-Fernández, Lorena Diéguez, Begoña Espiña, María Teresa Fernández-Argüelles
Carbon dots have demonstrated great potential as luminescent nanoparticles in bioapplications. Although such nanoparticles appear to exhibit low toxicity compared to other metal luminescent nanomaterials, today we know that the toxicity of carbon dots (C-dots) strongly depends on the protocol of fabrication. In this work, aqueous fluorescent C-dots have been synthesized from cinnamon, red chilli, turmeric and black pepper, by a one-pot green hydrothermal method. The synthesized C-dots were firstly characterized by means of UV-vis, fluorescence, Fourier transform infrared and Raman spectroscopy, dynamic light scattering and transmission electron microscopy...
2018: Beilstein Journal of Nanotechnology
Jiying Pei, Yinghui Wang, Kefu Yu
Quinones are a class of compounds having substantial toxicity and pharmacological function. This work has produced a derivatization method for quinone detection with high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). Through introducing tags (methoxy) to the quinone structures, the ionization efficiencies of five quinones (p-benzoquinone (BQ), methyl-p-benzoquinone (MBQ), 1,4-naphthoquinone (1,4-NQ), 1,2-naphthoquinone (1,2-NQ), and 1,4-anthraquinones (AQ)) were greatly improved during ESI...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Prashant S Devrukhakar, Shiva Shankar M, Shankar G, Srinivas R
Timolol (TIM) is a potent β-adrenergic blocker, useful in treatment of ocular hypertension or open-angle glaucoma. Development and validation of stability indicating LCMS assay method for TIM was accomplished coherent with ICH guideline. Successful chromatographic separation of TIM with its four degradation products was attained by using gradient elution mode on reverse phase column using ammonium acetate buffer, pH 4.6 as mobile phase A and organic solvent as the mobile phase B. Chromatographic conditions were set such as 1...
February 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
Deepak Bhandari, Brett A Bowman, Anish B Patel, David M Chambers, Víctor R De Jesús, Benjamin C Blount
This work describes a quantitative high-throughput analytical method for the simultaneous measurement of small aliphatic nitrogenous biomarkers, i.e., 1,6-hexamethylenediamine (HDA), isophoronediamine (IPDA), β-methylamino-l-alanine (BMAA), and trimethylamine N-oxide (TMAO), in human urine. Urinary aliphatic diamines, HDA and IPDA, are potential biomarkers of environmental exposure to their corresponding diisocyanates. Urinary BMAA forms as a result of human exposure to blue-green algae contaminated food. And, TMAO is excreted in urine due to the consumption of carnitine- and choline-rich diets...
March 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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