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confocal endomicroscopy

Shannon Melissa Chan, Anthony Yuen Bun Teoh, Hon Chi Yip, Vivien Wai Yin Wong, Philip Wai Yan Chiu, Enders Kwok Wai Ng
BACKGROUND AND AIMS: The recent development of endosonography (EUS)-guided gallbladder drainage (EGBD) with a lumen-apposing stent has made endoscopic assessment and advanced gallbladder interventions via the stent possible. The aim of this study was to assess the feasibility and safety of per-oral cholecystoscopy and the types of gallbladder interventions that can be performed. METHODS: This was a retrospective review conducted in the Prince of Wales Hospital from June 2012 to March 2016...
October 15, 2016: Gastrointestinal Endoscopy
Alexandra Schulz, Samira Daali, Mehreen Javed, Paul Christian Fuchs, Michael Brockmann, Alhadi Igressa, Patra Charalampaki
BACKGROUND: At present, no ideal diagnostic tools exist in the market to excise cancer tissue with the required safety margins and to achieve optimal aesthetic results using tissue-conserving techniques. OBJECTIVES: In this prospective study, confocal laser endomicroscopy (CLE) and the traditional gold standard of magnifying glasses (MG) were compared regarding the boundaries of in vivo basal cell carcinoma and squamous cell carcinoma. MATERIALS & METHODS: Tumour diameters defined by both methods were measured and compared with those determined by histopathological examination...
October 17, 2016: European Journal of Dermatology: EJD
Rohan M Modi, Amrit K Kamboj, Benjamin Swanson, Darwin L Conwell, Somashekar G Krishna
No abstract text is available yet for this article.
January 2016: Endoscopy
Tao Wu, Emilie Heuillard, Véronique Lindner, Ghina Bou About, Mihaela Ignat, Jean-Philippe Dillenseger, Nicolas Anton, Eugénie Dalimier, Francine Gossé, Gael Fouré, Franck Blindauer, Céline Giraudeau, Hussein El-Saghire, Mourad Bouhadjar, Cynthia Calligaro, Tania Sorg, Philippe Choquet, Thierry Vandamme, Christophe Ferrand, Jacques Marescaux, Thomas F Baumert, Michele Diana, Patrick Pessaux, Eric Robinet
The development of multimodal strategies for the treatment of hepatocellular carcinoma requires tractable animal models allowing for advanced in vivo imaging. Here, we characterize an orthotopic hepatocellular carcinoma model based on the injection of luciferase-expressing human hepatoma Huh-7 (Huh-7-Luc) cells in immunodeficient mice. Luciferase allows for an easy repeated monitoring of tumor growth by in vivo bioluminescence. The intrahepatic injection was more efficient than intrasplenic or intraportal injection in terms of survival, rate of orthotopic engraftment, and easiness...
October 14, 2016: Scientific Reports
Kazuki Sumiyama
Methodology for the diagnosis and staging of early gastric cancer (EGC) has improved in Japan since the development of the gastro-camera and determination of a definition of EGC. Imaging technology has been steadily evolving in the endoscopy field. Improvements in the resolution of standard endoscopy images used in screening and surveillance provide greater opportunities to find gastric cancer earlier. Image enhancement endoscopy (IEE), such as narrow band imaging (NBI), highlights mucosal structures and vascularity...
October 12, 2016: Gastric Cancer
James E East, Jasper L Vleugels, Philip Roelandt, Pradeep Bhandari, Raf Bisschops, Evelien Dekker, Cesare Hassan, Gareth Horgan, Ralf Kiesslich, Gaius Longcroft-Wheaton, Ana Wilson, Jean-Marc Dumonceau
Background and aim: This technical review is an official statement of the European Society of Gastrointestinal Endoscopy (ESGE). It addresses the utilization of advanced endoscopic imaging in gastrointestinal (GI) endoscopy. Methods: This technical review is based on a systematic literature search to evaluate the evidence supporting the use of advanced endoscopic imaging throughout the GI tract. Technologies considered include narrowed-spectrum endoscopy (narrow band imaging [NBI]; flexible spectral imaging color enhancement [FICE]; i-Scan digital contrast [I-SCAN]), autofluorescence imaging (AFI), and confocal laser endomicroscopy (CLE)...
October 6, 2016: Endoscopy
David C Adams, Lida P Hariri, Alyssa J Miller, Yan Wang, Josalyn L Cho, Martin Villiger, Jasmin A Holz, Margit V Szabari, Daniel L Hamilos, R Scott Harris, Jason W Griffith, Brett E Bouma, Andrew D Luster, Benjamin D Medoff, Melissa J Suter
The inability to visualize airway smooth muscle (ASM) cells in vivo is a major obstacle in understanding their role in normal physiology and diseases. At present, there is no imaging modality available to assess ASM in vivo. Confocal endomicroscopy lacks the penetration depth and field of view, and conventional optical coherence tomography (OCT) does not have sufficient contrast to differentiate ASM from surrounding tissues. We have developed a birefringence microscopy platform that leverages the micro-organization of tissue to add further dimension to traditional OCT...
October 5, 2016: Science Translational Medicine
C Ba, M Palmiere, J Ritt, J Mertz
We describe a dual-modality laser scanning endomicroscope that provides simultaneous fluorescence contrast based on confocal laser endomicroscopy (CLE) and phase-gradient contrast based on scanning oblique back-scattering microscopy (sOBM). The probe consists of a 2.6mm-diameter micro-objective attached to a 30,000-core flexible fiber bundle. The dual contrasts are inherently co-registered, providing complementary information on labeled and un-labeled sample structure. Proof of principle demonstrations are presented with ex-vivo mouse colon tissue...
September 1, 2016: Biomedical Optics Express
Adriana Vaz Safatle-Ribeiro, Elisa Ryoka Baba, Sheila Friedrich Faraj, Juliana Trazzi Rios, Marcelo Simas de Lima, Bruno Costa Martins, Sebastian Naschold Geiger, Caterina Pennacchi, Carla Gusman, Fábio Shiguehissa Kawaguti, Ricardo Sato Uemura, Evandro Sobroza de Melo, Ulysses Ribeiro Junior, Fauze Maluf-Filho
BACKGROUND AND AIMS: Surveillance programs of patients with head and neck cancer (HNC) detect synchronous or metachronous esophageal squamous cell carcinoma (ESCC) in up to 15% of the patients. Non-invasive probe-based confocal laser endomicroscopy (pCLE) technique may improve the diagnosis allowing acquisition of high-resolution in vivo images at the cellular and microvascular levels. The aim was to evaluate the accuracy of pCLE for the differential diagnosis of non-neoplastic and neoplastic Lugol-unstained esophageal lesions in patients with HNC...
September 30, 2016: Gastrointestinal Endoscopy
B Njei, T R McCarty, S Varadarajulu, U Navaneethan
BACKGROUND: The accuracy of current endoscopic modalities for diagnosing cholangiocarcinoma in primary sclerosing cholangitis (PSC) is suboptimal. AIM: To evaluate the comparative effectiveness of endoscopic retrograde cholangiopancreatography (ERCP)-based modalities, independently or in combination, for the diagnosis of cholangiocarcinoma in patients with PSC-induced biliary strictures. METHODS: Searches of PubMed, EMBASE, Web of Science and the Cochrane Library databases were performed through December 2015...
October 3, 2016: Alimentary Pharmacology & Therapeutics
Edoardo Savarino, Andrea Ottonello, Salvatore Tolone, Ottavia Bartolo, Myong Ki Baeg, Farhood Farjah, Shiko Kuribayashi, Katerina P Shetler, Christian Lottrup, Ellen Stein
The 21st century offers new advances in diagnostic procedures and protocols in the management of esophageal diseases. This review highlights the most recent advances in esophageal diagnostic technologies, including clinical applications of novel endoscopic devices, such as ultrathin endoscopy and confocal laser endomicroscopy for diagnosis and management of Barrett's esophagus; novel parameters and protocols in high-resolution esophageal manometry for the identification and better classification of motility abnormalities; innovative connections between esophageal motility disorder diagnosis and detection of gastroesophageal reflux disease (GERD); impedance-pH testing for detecting the various GERD phenotypes; performance of distensibility testing for better pathophysiological knowledge of the esophagus and other gastrointestinal abnormalities; and a modern view of positron emission tomography scanning in metastatic disease detection in the era of accountability as a model for examining other new technologies...
September 28, 2016: Annals of the New York Academy of Sciences
L G Rossini, L R Meirelles, S M Reimao, J L Paccos, S S Filippi, C M Ribeiro, J Valenciano, F P Martins, J C Cassab, R Coudry
No abstract text is available yet for this article.
November 2015: Journal of Minimally Invasive Gynecology
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Endoscopy
Angelo Pierangelo, David Fuks, Pierre Validire, Ali Benali, Brice Gayet
OBJECTIVES: Probe-based confocal laser endomicroscopy (pCLE) is a promising new imaging technique enabling in-vivo analysis of tissues at the cellular level, in real time. The aim of the present study was to prospectively evaluate the feasibility and accuracy of indocyanine green-aided pCLE for the diagnosis of malignant liver nodules. PATIENTS AND METHODS: From October 2014 to July 2015, liver specimens from 30 consecutive patients were analyzed ex vivo using pCLE directly after resection, with indocyanine green as contrast agent...
September 22, 2016: European Journal of Gastroenterology & Hepatology
Vladislav Pavlov, David Meyronet, Vincent Meyer-Bisch, Xavier Armoiry, Brian Pikul, Chloé Dumot, Pierre-Aurelien Beuriat, Francesco Signorelli, Jacques Guyotat
BACKGROUND: The management of gliomas is based on precise histologic diagnosis. The tumor tissue can be obtained during open surgery or via stereotactic biopsy. Intraoperative tissue imaging could substantially improve biopsy precision and, ultimately, the extent of resection. OBJECTIVE: To show the feasibility of intraoperative in vivo probe-based confocal laser endomicroscopy (pCLE) in surgery and biopsy of gliomas. METHODS: In our prospective observational study, 9 adult patients were enrolled between September 2014 and January 2015...
October 2016: Neurosurgery
Kunal Karia, Michel Kahaleh
Confocal laser endomicroscopy (CLE) is a novel in vivo imaging technique that can provide real-time optical biopsies in the evaluation of pancreaticobiliary strictures and pancreatic cystic lesions (PCLs), both of which are plagued by low sensitivities of routine evaluation techniques. Compared to pathology alone, CLE is associated with a higher sensitivity and accuracy for the evaluation of indeterminate pancreaticobiliary strictures. CLE has the ability to determine the malignant potential of PCLs. As such, CLE can increase the diagnostic yield of endoscopic retrograde cholangiopancreatography and endoscopic ultrasound, reducing the need for repeat procedures...
September 2016: Clinical Endoscopy
Dongkyun Kang, Simon C Schlachter, Robert W Carruth, Minkyu Kim, Tao Wu, Nima Tabatabaei, Amna R Soomro, Catriona N Grant, Mireille Rosenberg, Norman S Nishioka, Guillermo J Tearney
BACKGROUND AND OBJECTIVE: Diagnosis of esophageal diseases is often hampered by sampling errors that are inherent in endoscopic biopsy, the standard of care. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal endomicroscopy technology that has the potential to visualize cellular features from large regions of the esophagus, greatly decreasing the likelihood of sampling error. In this paper, we report results from a pilot clinical study imaging the human esophagus in vivo with a prototype SECM endoscopic probe...
September 16, 2016: Lasers in Surgery and Medicine
Anna M Buchner, Michael B Wallace
Endoscopy is an essential tool for effective care of patients with inflammatory bowel disease (IBD), including Crohn disease and ulcerative colitis. The newest endoscopic small-field imaging technologies with confocal endomicroscopy have allowed real-time imaging of gastrointestinal mucosal during ongoing endoscopic evaluation and in vivo histology. Thus, endomicroscopy has a potential to further enhance the endoscopic evaluation of IBD. Advances in molecular in vivo imaging in IBD may be used not only to better understand the pathophysiology of IBD but also to guide optimized therapy and thus to allow a personalized, new approach to the IBD management...
October 2016: Gastrointestinal Endoscopy Clinics of North America
Cong-Qiu Chu
Therapy by blocking tumor necrosis factor (TNF) activity is highly efficacious and profoundly changed the paradigm of several inflammatory diseases. However, a significant proportion of patients with inflammatory diseases do not respond to TNF inhibitors (TNFi). Prediction of therapeutic response is required for TNFi therapy. Isotope labeled anti-TNF antibodies or TNF receptor have been investigated to localize TNF production at inflammatory tissue in animal models and in patients with inflammatory diseases...
September 12, 2016: Cytokine
Zhen Zhang, Ming Li, Feixue Chen, Lixiang Li, Jun Liu, Zhen Li, Rui Ji, Xiuli Zuo, Yanqing Li
INTRODUCTION: Mesenchymal stem cells (MSCs) can serve as vehicles for therapeutic genes. However, little is known about MSC behavior in vivo. Here, we demonstrated that probe-based confocal laser endomicroscopy (pCLE) can be used to track MSCs in vivo and individually monitor tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) gene expression within carcinomas. METHODS: Isolated BALB/c nu/nu mice MSCs (MSCs) were characterized and engineered to co-express the TRAIL and enhanced green fluorescent protein (EGFP) genes...
2016: PloS One
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