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Quantum barcode

Chiara Bedin, Sara Crotti, Ennio Tasciotti, Marco Agostini
Nowadays, fast and sensitive methods for biomarkers detection exist, but the performance of most of them still rely in centralized laboratory testing. The development of small, fast and simple to use medical device that can help in making diagnosis accurate and with low-invasiveness is now a major challenge for nanotechnology. To develop diagnostic devices for cancer detection, nanomaterials-based systems have significant advantages over current conventional approaches in terms of simplicity, sensitivity, specificity and rapidity...
November 16, 2017: Current Medicinal Chemistry
Bohdan Andreiuk, Andreas Reisch, Marion Lindecker, Gautier Follain, Nadine Peyriéras, Jacky G Goetz, Andrey S Klymchenko
Fluorescent polymer nanoparticles for long-term labeling and tracking of living cells with any desired color code are developed. They are built from biodegradable poly(lactic-co-glycolic acid) polymer loaded with cyanine dyes (DiO, DiI, and DiD) with the help of bulky fluorinated counterions, which minimize aggregation-caused quenching. At the single particle level, these particles are ≈20-fold brighter than quantum dots of similar color. Due to their identical 40 nm size and surface properties, these nanoparticles are endocytosed equally well by living cells...
August 9, 2017: Small
David X Medina, Kyle T Householder, Ricki Ceton, Tina Kovalik, John M Heffernan, Rohini V Shankar, Robert P Bowser, Robert J Wechsler-Reya, Rachael W Sirianni
Understanding of the mechanisms by which systemically administered nanoparticles achieve delivery across biological barriers remains incomplete, due in part to the challenge of tracking nanoparticle fate in the body. Here, we develop a new approach for "barcoding" nanoparticles composed of poly(lactic-co-glycolic acid) (PLGA) with bright, spectrally defined quantum dots (QDs) to enable direct, fluorescent detection of nanoparticle fate with subcellular resolution. We show that QD labeling does not affect major biophysical properties of nanoparticles or their interaction with cells and tissues...
May 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
Kristyna Cihalova, Dagmar Hegerova, Ana Maria Jimenez, Vedran Milosavljevic, Jiri Kudr, Sylvie Skalickova, David Hynek, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam
Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots)...
February 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
Jisung Kim, Mia J Biondi, Jordan J Feld, Warren C W Chan
There has been a major focus on the clinical translation of emerging technologies for diagnosing patients with infectious diseases, cancer, heart disease, and diabetes. However, most developments still remain at the academic stage where researchers use spiked target molecules to demonstrate the utility of a technology and assess the analytical performance. This approach does not account for the biological complexities and variabilities of human patient samples. As a technology matures and potentially becomes clinically viable, one important intermediate step in the translation process is to conduct a full clinical validation of the technology using a large number of patient samples...
April 26, 2016: ACS Nano
Yu Zheng, Kan Wang, Jingjing Zhang, Weijian Qin, Xinyu Yan, Guangxia Shen, Guo Gao, Fei Pan, Daxiang Cui
Quantum dots-labeled urea-enzyme antibody-based rapid immunochromatographic test strips have been developed as quantitative fluorescence point-of-care tests (POCTs) to detect helicobacter pylori. Presented in this study is a new test strip reader designed to run on tablet personal computers (PCs), which is portable for outdoor detection even without an alternating current (AC) power supply. A Wi-Fi module was integrated into the reader to improve its portability. Patient information was loaded by a barcode scanner, and an application designed to run on tablet PCs was developed to handle the acquired images...
December 2016: Nanoscale Research Letters
Jinyang Chen, Xinghu Ji, Zhike He
In this work, a simple, flexible and low-cost sample-introduction technique was developed and integrated with droplet platform. The sample-introduction strategy was realized based on connecting the components of positive pressure input device, sample container and microfluidic chip through the tygon tubing with homemade polydimethylsiloxane (PDMS) adaptor, so the sample was delivered into the microchip from the sample container under the driving of positive pressure. This sample-introduction technique is so robust and compatible that could be integrated with T-junction, flow-focus or valve-assisted droplet microchips...
August 12, 2015: Analytica Chimica Acta
Christian Würth, Ute Resch-Genger
The ever-increasing use of fluorescent nanomaterials and micrometer-sized beads in the life and material sciences requires reliable procedures for the measurement of the key performance parameter fluorescence quantum yield (Φf) of scattering particle dispersions and reference systems to evaluate the performance of such measurements. This encouraged us to systematically study, both theoretically and experimentally, the optical determination of photoluminescent quantum yield as a function of the scattering and absorption properties of the sample and the illumination geometry with an integrating sphere method...
June 2015: Applied Spectroscopy
Ning Li, Xiaodi Su, Yi Lu
Biosensors incorporating nanomaterials have demonstrated superior performance compared to their conventional counterparts. Most reported sensors use nanomaterials as a single transducer of signals, while biosensor designs using dual transducing elements have emerged as new approaches to further improve overall sensing performance. This review focuses on recent developments in nanomaterial-based biosensors using dual transducing elements for solution phase detection. The review begins with a brief introduction of the commonly used nanomaterial transducers suitable for designing dual element sensors, including quantum dots, metal nanoparticles, upconversion nanoparticles, graphene, graphene oxide, carbon nanotubes, and carbon nanodots...
May 7, 2015: Analyst
Shyam V Vaidya, Alex Couzis, Charles Maldarelli
We report the development of barcoded polystyrene microbeads, approximately 50 μm in diameter, which are encoded by incorporating multicolored semiconductor fluorescent nanocrystals (quantum dots or QDs) within the microbeads and using the emission spectrum of the embedded QDs as a spectral label. The polymer/nanocrystal bead composites are formed by polymerizing emulsified liquid droplets of styrene monomer and QDs suspended in an immiscible continuous phase (suspension polymerization). We focus specifically on the effect of divinylbenzene (DVB) added to cross-link the linearly growing styrene polymer chains and the effect of this cross-linking on the state of aggregation of the nanocrystals in the composite...
March 17, 2015: Langmuir: the ACS Journal of Surfaces and Colloids
Kevin Ming, Jisung Kim, Mia J Biondi, Abdullah Syed, Kun Chen, Albert Lam, Mario Ostrowski, Anu Rebbapragada, Jordan J Feld, Warren C W Chan
Inorganic nanoparticles are ideal precursors for engineering barcodes for rapidly detecting diseases. Despite advances in the chemical design of these barcodes, they have not advanced to clinical use because they lack sensitivity and are not cost-effective due to requirement of a large read-out system. Here we combined recent advances in quantum dot barcode technology with smartphones and isothermal amplification to engineer a simple and low-cost chip-based wireless multiplex diagnostic device. We characterized the analytical performance of this device and demonstrated that the device is capable of detecting down to 1000 viral genetic copies per milliliter, and this enabled the diagnosis of patients infected with HIV or hepatitis B...
March 24, 2015: ACS Nano
Kelin Xia, Xin Feng, Yiying Tong, Guo Wei Wei
Persistent homology is a relatively new tool often used for qualitative analysis of intrinsic topological features in images and data originated from scientific and engineering applications. In this article, we report novel quantitative predictions of the energy and stability of fullerene molecules, the very first attempt in using persistent homology in this context. The ground-state structures of a series of small fullerene molecules are first investigated with the standard Vietoris-Rips complex. We decipher all the barcodes, including both short-lived local bars and long-lived global bars arising from topological invariants, and associate them with fullerene structural details...
March 5, 2015: Journal of Computational Chemistry
Jianbo Liu, Gui Li, Xiaohai Yang, Kemin Wang, Li Li, Wei Liu, Xing Shi, Yali Guo
A novel exciton energy transfer-based fluorescence sensing array for the discrimination of different nucleobases was developed through target nucleobase-triggered self-assembly of quantum dots (QDs). Four QD nanoprobes with different ligand receptors, including mercaptoethylamine, N-acetyl-l-cysteine, 2-dimethyl-aminethanethiol, and thioglycolic acid, were created to detect and identify nucleobase targets. These QDs served as both selective recognition scaffolds and signal transduction elements for a biomolecule target...
January 20, 2015: Analytical Chemistry
Omid Mashinchian, Mohammad Johari-Ahar, Behnaz Ghaemi, Mohammad Rashidi, Jaleh Barar, Yadollah Omidi
INTRODUCTION: A number of assays have so far been exploited for detection of cancer biomarkers in various malignancies. However, the expression of cancer biomarker(s) appears to be extremely low, therefore accurate detection demands sensitive optical imaging probes. While optical detection using conventional fluorophores often fail due to photobleaching problems, quantum dots (QDs) offer stable optical imaging in vitro and in vivo. METHODS: In this review, we briefly overview the impacts of QDs in biology and its applications in bioimaging of malignancies...
2014: BioImpacts: BI
Yang Chen, Peng-Fei Dong, Jian-Hong Xu, Guang-Sheng Luo
A novel microfluidic approach is developed to prepare multicolor QDs-encoded core-shell microparticles with precise and various barcode and enhanced stability performance. With the protection of the hydrogel shell, the leakage of QDs is avoided and the fluorescent stability is enhanced greatly. By embedding different QDs into different cores, no interaction between different QDs existed and the fluorescence spectrum of each kind of QDs can be recorded, respectively. Compared with QDs mixtures in a single particle, it is unnecessary to separate the emissions of QDs in different colors, and deconvolution algorithms are not needed...
July 22, 2014: Langmuir: the ACS Journal of Surfaces and Colloids
Yao Cheng, Yuanjin Zhao, Fengqi Shangguan, Baofen Ye, Tingting Li, Zhongze Gu
Barcode particles have a demonstrated value for multiplexed bioassays in clinical diagnostics, drug discovery, and so on. Attempts to develop this technology are focusing on the generation of novel particles with a large number of identifications to increase the throughput of the assays. Here we report a new type of barcode particles with quantum dot (QD)-photonic crystal (PC) joint spectrum encoding. These barcode particles are simply prepared by in-situ polymerization of CdTe QDs-contained acrylamide pre-gel in the colloidal photonic crystal beads (PCBs)...
May 2014: Journal of Biomedical Nanotechnology
Feng Qiu, James B McAlpine, David C Lankin, Ian Burton, Tobias Karakach, Shao-Nong Chen, Guido F Pauli
The interpretation of NMR spectroscopic information for structure elucidation involves decoding of complex resonance patterns that contain valuable molecular information (δ and J), which is not readily accessible otherwise. We introduce a new concept of 2D-NMR barcoding that uses clusters of fingerprint signals and their spatial relationships in the δ-δ coordinate space to facilitate the chemical identification of complex mixtures. Similar to widely used general barcoding technology, the structural information of individual compounds is encoded as a specifics pattern of their C,H correlation signals...
April 15, 2014: Analytical Chemistry
Tao Song, Junqing Liu, Wenbin Li, Yunhong Li, Qiuhua Yang, Xiaoqun Gong, Lixue Xuan, Jin Chang
Quantum dot (QD) barcodes are becoming an urgent requirement for researchers and clinicians to obtain high-density information in multiplexed suspension (bead-based) assay. However, how to improve the stability of quantum dot barcodes is a longstanding issue. Here, we present a new self-healing encapsulation strategy to generate functionalized uniform quantum dots barcodes with high physical and chemical stability. This efficient and facile strategy could make porous polymer microspheres self-heal to encapsulate QDs via the thermal motion and interaction of the molecular chains...
February 26, 2014: ACS Applied Materials & Interfaces
Elizabeth Csaszar, Kun Chen, Julia Caldwell, Warren Chan, Peter W Zandstra
Monitoring and control of primary cell cultures is challenging as they are heterogenous and dynamically complex systems. Feedback signaling proteins produced from off-target cell populations can accumulate, inhibiting the production of the desired cell populations. Although culture strategies have been developed to reduce feedback inhibition, they are typically optimized for a narrow range of process parameters and do not allow for a dynamically regulated response. Here we describe the development of a microbead-based process control system for the monitoring and control of endogenously produced signaling factors...
June 2014: Biotechnology and Bioengineering
Wenyuan Zhu, Xingpeng Su, Xiaoyu Gao, Zong Dai, Xiaoyong Zou
The profiling of microRNAs (miRNAs) is greatly significant for cellular events or disease diagnosis. Electrochemical methods for miRNAs analysis mostly can only measure one kind of miRNA, which is unambiguous to indicate the disease type and state. Here a label-free and PCR-free electrochemical method is presented for multiplexed evaluation of miRNAs in a single-tube experiment. The method is based on the combination of the high base-mismatch selectivity of ligase chain reaction (LCR) and the remarkable voltammetric signature of electrochemical QDs barcodes...
March 15, 2014: Biosensors & Bioelectronics
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