keyword
MENU ▼
Read by QxMD icon Read
search

frizzled

keyword
https://www.readbyqxmd.com/read/28438350/microrna-505-modulates-cancer-proliferation-and-migration-in-human-non-small-cell-lung-cancer-through-inverse-regulation-of-fzd4
#1
Hong Li, Tian Yang, Qian Ning, Dong Shang, Yan Yao, Zhongmin Sun
OBJECTIVES: The current study is to evaluate the functional role of microRNA-505 (miR-505) in human non-small cell lung cancer (NSCLC). METHODS: Quantitative RT-PCR was performed to examine miR-505 expression in tumors of NSCLC patients and NSCLC cell lines. Lentiviruses of miR-505 mimics and miR-505 inhibitor were used to upregulate or downregulate miR-505 in NSCLC cell lines, A549 and H810 cells. Their effects on NSCLC in vitro growth, migration and in vivo growth were evaluated by proliferation, migration and tumorigenicity assays, respectively...
March 31, 2017: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/28427856/frizzled-3-acts-upstream-of-alcam-during-embryonic-eye-development
#2
Franziska A Seigfried, Wiebke Cizelsky, Astrid S Pfister, Petra Dietmann, Paul Walther, Michael Kühl, Susanne J Kühl
Formation of a functional eye during vertebrate embryogenesis requires different processes such as cell migration, cell-cell interactions as well as intracellular signalling processes. It was previously shown that the non-canonical Wnt receptor Frizzled 3 (Fzd3) is required for proper eye formation, however, the underlying mechanism is poorly understood. Here we demonstrate that loss of Fzd3 induces severe malformations of the developing eye and that this defect is phenocopied by loss of the activated leukocyte cell adhesion molecule (Alcam)...
April 17, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28425477/microrna-27a-3p-modulates-the-wnt-%C3%AE-catenin-signaling-pathway-to-promote-epithelial-mesenchymal-transition-in-oral-squamous-carcinoma-stem-cells-by-targeting-sfrp1
#3
Bin Qiao, Bao-Xia He, Jing-Hua Cai, Qian Tao, Alfred King-Yin Lam
This study aimed to elucidate how microRNA27a-3p (miR-27a-3p) modulates the Wnt/β-catenin signaling pathway to promote the epithelial-mesenchymal transition (EMT) in oral squamous carcinoma stem cells (OSCSCs) by targeting secreted frizzled-related protein 1 (SFRP1). Flow cytometry was used to sort OSCSCs from the SCC-9 and Tca8113 cell lines. The OSCSCs were randomly assigned into the miR-27a-3p inhibitors group, the miR-27a-3p inhibitors-NC group, the si-SFRP1 group, the si-SFRP1 + miR-27a-3p inhibitors group and the blank group...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418908/plasma-protein-profiling-in-patients-undergoing-coronary-artery-bypass-grafting-surgery-and-clinical-significance
#4
Zhi-Peng Guo, Hai-Tao Hou, Rui Jing, Zhen-Guo Song, Xiao-Cheng Liu, Guo-Wei He
This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60)...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418856/low-density-lipoprotein-receptor-related-protein-6-is-a-novel-coreceptor-of-protease-activated-receptor-2-in-the-dynamics-of-cancer-associated-%C3%AE-catenin-stabilization
#5
Jeetendra Kumar Nag, Arun Kancharla, Myriam Maoz, Hagit Turm, Daniel Agranovich, Chhedi Lal Gupta, Beatrice Uziely, Rachel Bar-Shavit
Protease-activated receptor-2 (PAR2) plays a central role in cancer; however, the molecular machinery of PAR2-instigated tumors remains to be elucidated. We show that PAR2 is a potent inducer of β-catenin stabilization, a core process in cancer biology, leading to its transcriptional activity. Novel association of low-density lipoprotein-related protein 6 (LRP6), a known coreceptor of Frizzleds (Fz), with PAR2 takes place following PAR2 activation. The association between PAR2 and LRP6 was demonstrated employing co-immunoprecipitation, bioluminescence resonance energy transfer (BRET), and confocal microscopy analysis...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28411232/wnt5a-regulates-adipose-tissue-angiogenesis-via-anti-angiogenic-vegfa165b-in-obese-humans
#6
Shakun Karki, Doan Ngo, Melissa G Farb, Song-Young Park, Samantha M Saggese, Naomi M Hamburg, Brian Carmine, Donald T Hess, Kenneth Walsh, Noyan Gokce
Experimental studies suggest that Wingless-related integration site 5a (WNT5A) is a pro-inflammatory secreted protein that is associated with metabolic dysfunction in obesity. Impaired angiogenesis in fat has been implicated in the development of adipose tissue capillary rarefaction, hypoxia, inflammation and metabolic dysfunction. We recently demonstrated that impaired adipose tissue angiogenesis is associated with overexpression of anti-angiogenic factor VEGF-A165b in human fat and the systemic circulation...
April 14, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28403963/the-wnt-%C3%AE-catenin-signaling-pathway-may-be-involved-in-granulosa-cell-apoptosis-from-patients-with-pcos-in-north-china
#7
X-Q Wu, Y-Q Wang, S-M Xu, J-F Liu, X-Y Bi, Z-Q Wang, J-P Zhang
INTRODUCTION: Investigate the expressions of WNTs in granulosa cells of PCOS in North China, and explore the possible mechanism. MATERIAL AND METHODS: Patients with PCOS (n=40) and controls without PCOS (n=20) were enrolled into the study. The levels of serum sex hormone were detected by chemiluminescent enzyme immunoassay. RT-qPCR was used to measure mRNA levels of WNT family members (WNT1, WNT3, WNT4, WNT5, sFRP4 and sFRP5). The levels of Bax and Bcl-2 were measured by enzyme-linked immunosorbent assay...
January 2017: J Gynecol Obstet Hum Reprod
https://www.readbyqxmd.com/read/28398872/hydrogen-peroxide-induced-secreted-frizzled-related-protein-1-gene-demethylation-contributes-to-hydrogen-peroxide-induced-apoptosis-in-human-u251-glioma-cells
#8
Zhiguo Xing, Yaping Ni, Junjie Zhao, Xudong Ma
Glioblastoma multiforme is a type of central nervous system tumor with extremely poor prognosis. Previously, hydrogen peroxide (H2O2), which promotes the oxidative stress response, has been reported to induce the apoptosis of glioma cells. Recently, secreted frizzled-related protein 1 (SFRP1) has been shown to be associated with various types of malignant tumors and with H2O2-induced oxidative stress in cardiomyocytes by negatively regulating the Wnt signaling pathway. This study aimed to explore SFRP1 expression and its roles in H2O2-induced apoptosis in human glioma cells...
February 28, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28386775/intermittent-pacing-therapy-favorably-modulates-infarct-remodeling
#9
André Uitterdijk, Tirza Springeling, Kevin C M Hermans, Daphne Merkus, Vincent J de Beer, Charlotte Gorsse-Bakker, Eric Mokelke, Evangelos P Daskalopoulos, Piotr A Wielopolski, Jack P M Cleutjens, W Matthijs Blankesteijn, Frits W Prinzen, Willem J van der Giessen, Robert-Jan M van Geuns, Dirk J Duncker
Despite early revascularization, remodeling and dysfunction of the left ventricle (LV) after acute myocardial infarction (AMI) remain important therapeutic targets. Intermittent pacing therapy (IPT) of the LV can limit infarct size, when applied during early reperfusion. However, the effects of IPT on post-AMI LV remodeling and infarct healing are unknown. We therefore investigated the effects of IPT on global LV remodeling and infarct geometry in swine with a 3-day old AMI. For this purpose, fifteen pigs underwent 2 h ligation of the left circumflex coronary artery followed by reperfusion...
May 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28385590/characterization-and-expression-pattern-of-a-novel-frizzled-8-receptor-gene-in-schistosoma-japonicum
#10
Jingxiu Xu, Xingang Feng, Yingying Jia, Yang Hong, Hao Li, Ke Lu, Jiaojiao Lin, Chunxiu Yuan, Mingxin Song
Wnt signaling as mediated by the Frizzled family receptors plays a vital role in the early development of animal embryos, organ formation, tissue regeneration and other physiological processes. In the present study, a novel Frizzled member, SjFz8, was isolated and characterized in Schistosoma japonicum. SjFz8 encodes an 1162-amino-acid protein with typical characteristics of Frizzled proteins. Quantitative real-time polymerase chain reaction analysis indicated that SjFz8 transcript level was highest in 7-day-old schistosomula...
April 3, 2017: Parasitology International
https://www.readbyqxmd.com/read/28384160/a-wnt-planar-polarity-pathway-instructs-neurite-branching-by-restricting-f-actin-assembly-through-endosomal-signaling
#11
Chun-Hao Chen, Chun-Wei He, Chien-Po Liao, Chun-Liang Pan
Spatial arrangement of neurite branching is instructed by both attractive and repulsive cues. Here we show that in C. elegans, the Wnt family of secreted glycoproteins specify neurite branching sites in the PLM mechanosensory neurons. Wnts function through MIG-1/Frizzled and the planar cell polarity protein (PCP) VANG-1/Strabismus/Vangl2 to restrict the formation of F-actin patches, which mark branching sites in nascent neurites. We find that VANG-1 promotes Wnt signaling by facilitating Frizzled endocytosis and genetically acts in a common pathway with arr-1/β-arrestin, whose mutation results in defective PLM branching and F-actin patterns similar to those in the Wnt, mig-1 or vang-1 mutants...
April 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28382864/microrna-130b-promotes-wear-particle-induced-osteolysis-via-downregulating-frizzled-related-protein-frzb
#12
De-Zhi Zheng, Yan-Min Bu, Lei Wang, Jun Liu
Periprosthetic osteolysis induced by wear particles can lead to aseptic loosening, one main reason of arthroplasty failure. However, the role of microRNA-130b (miR-130b) in particle-induced osteolysis (PIO) has not been explored yet. In this study, PIO models were established in C57BL/J6 mice via the implantation of Co-Cr-Mo alloy particles, and evaluated by detecting tartrate-resistant acid phosphatase (TRAP) activity and bone resorption in the calvaria. Mouse preosteoblast MC3T3-E1 cells were cultured to receive particle stimulation in vitro...
2017: Current Neurovascular Research
https://www.readbyqxmd.com/read/28377511/unsaturated-fatty-acyl-recognition-by-frizzled-receptors-mediates-dimerization-upon-wnt-ligand-binding
#13
Aaron H Nile, Susmith Mukund, Karen Stanger, Weiru Wang, Rami N Hannoush
Frizzled (FZD) receptors mediate Wnt signaling in diverse processes ranging from bone growth to stem cell activity. Moreover, high FZD receptor expression at the cell surface contributes to overactive Wnt signaling in subsets of pancreatic, ovarian, gastric, and colorectal tumors. Despite the progress in biochemical understanding of Wnt-FZD receptor interactions, the molecular basis for recognition of Wnt cis-unsaturated fatty acyl groups by the cysteine-rich domain (CRD) of FZD receptors remains elusive. Here, we determined a crystal structure of human FZD7 CRD unexpectedly bound to a 24-carbon fatty acid...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28373995/dickkopf-1-is-a-biomarker-for-systemic-lupus-erythematosus-and-active-lupus-nephritis
#14
Jing Xue, Jiali Yang, Lijuan Yang, Shaolan Zhou, Chen Ji, Xuemei Wang, Nan Yu, Xiaoming Liu, Shuhong Chi
An early diagnosis of lupus nephritis (LN) has an important clinical implication in guiding treatments of systemic lupus erythematosus (SLE) in clinical settings. In this study, the concentrations of Wnt-3A, Frizzled-8 (FZD-8), and Dickkopf-1 (DKK-1) of Wnt signaling, as well as their diagnostic values for accessing LN, were evaluated by ELISA in sera and urine of 111 SLE patients (31 with LN and 80 without LN) and 70 healthy cohorts. Significantly more abundances of DKK-1 protein were determined in both of sera and urine of SLE patients compared to healthy cohorts (p < 0...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28373842/breast-cancer-stem-like-cells-are-inhibited-by-diosgenin-a-steroidal-saponin-by-the-attenuation-of-the-wnt-%C3%AE-catenin-signaling-via-the-wnt-antagonist-secreted-frizzled-related-protein-4
#15
G Bhuvanalakshmi, Basappa, Kanchugarakoppal S Rangappa, Arun Dharmarajan, Gautam Sethi, Alan P Kumar, Sudha Warrier
Background: Identification of breast cancer stem cells as the chemo-resistant and tumor-initiating population represents an important milestone in approaching anticancer therapies. Targeting this minor subpopulation of chemo- and radio-resistant stem-like cells, termed as the cancer stem cells (CSCs) and their eradication could significantly enhance clinical outcomes. Most of the presently administered chemotherapeutics target the tumor bulk but are ineffective against the CSCs. We report here that diosgenin (DG), a naturally occurring steroidal saponin, could effectively inhibit CSCs from three breast cancer cell lines, MCF7, T47D and MDA-MB-231, by inducing apoptosis and inhibiting the CSC associated phenotypes...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28368424/p53-rb-inhibition-induces-metastatic-adrenocortical-carcinomas-in-a-preclinical-transgenic-model
#16
M Batisse-Lignier, I Sahut-Barnola, F Tissier, T Dumontet, M Mathieu, C Drelon, J-C Pointud, C Damon-Soubeyrand, G Marceau, J-L Kemeny, J Bertherat, I Tauveron, P Val, A Martinez, A-M Lefrançois-Martinez
Adrenocortical carcinoma (ACC) is a rare cancer with poor prognosis. Pan-genomic analyses identified p53/Rb and WNT/β-catenin signaling pathways as main contributors to the disease. However, isolated β-catenin constitutive activation failed to induce malignant progression in mouse adrenocortical tumors. Therefore, there still was a need for a relevant animal model to study ACC pathogenesis and to test new therapeutic approaches. Here, we have developed a transgenic mice model with adrenocortical specific expression of SV40 large T-antigen (AdTAg mice), to test the oncogenic potential of p53/Rb inhibition in the adrenal gland...
April 3, 2017: Oncogene
https://www.readbyqxmd.com/read/28359679/activation-of-wnt-signaling-increases-numbers-of-enteric-neurons-derived-from-neonatal-mouse-and-human-progenitors-cells
#17
Ying Zhang, Karin Seid, Florian Obermayr, Lothar Just, Peter H Neckel
BACKGROUND & AIMS: Neural stem and progenitor cells from the enteric nervous system (ENS) might serve as a source of cells for treatment of neurogastrointestinal disorders. Before we can use these cells, we must increase our understanding of the signaling mechanisms that regulate proliferation and differentiation. We systematically evaluated the effects of canonical Wnt signaling on proliferation and differentiation of cultured ENS progenitor cells from neonatal mice and humans. METHODS: We isolated ENS progenitors from tunica muscularis of the small intestine of new-born (P0) wild-type C57BL/6 mice as well as from Wnt1-Cre2 reporter mice...
March 27, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28356706/a-mutagenesis-derived-lrp5-mouse-mutant-with-abnormal-retinal-vasculature-and-low-bone-mineral-density
#18
Jeremy R Charette, Sarah E Earp, Brent A Bell, Cheryl L Ackert-Bicknell, Dana A Godfrey, Sujata Rao, Bela Anand-Apte, Patsy M Nishina, Neal S Peachey
PURPOSE: Familial exudative vitreoretinopathy (FEVR) is caused by mutations in the genes encoding low-density lipoprotein receptor-related protein (LRP5) or its interacting partners, namely frizzled class receptor 4 (FZD4) and norrin cystine knot growth factor (NDP). Mouse models for Lrp5, Fzd4, and Ndp have proven to be important for understanding the retinal pathophysiology underlying FEVR and systemic abnormalities related to defective Wnt signaling. Here, we report a new mouse mutant, tvrm111B, which was identified by electroretinogram (ERG) screening of mice generated in the Jackson Laboratory Translational Vision Research Models (TVRM) mutagenesis program...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28356444/antibody-mediated-inhibition-of-tspan12-ameliorates-vasoproliferative-retinopathy-through-suppression-of-%C3%AE-catenin-signaling
#19
Felicitas Bucher, Ding Zhang, Edith Aguilar, Susumu Sakimoto, Sophia Diaz-Aguilar, Mauricio Rosenfeld, Zhao Zha, Hongkai Zhang, Martin Friedlander, Kyungmoo Yea
Background -Anti-angiogenic biologicals represent an important concept for the treatment of vasoproliferative diseases. However, the need for continued treatment, the presence of non-responders and the risk of long-term side-effects limit the success of existing therapeutic agents. While Tspan12 has been shown to regulate retinal vascular development, nothing is known about its involvement in neovascular disease and its potential as novel therapeutic target for the treatment of vasoproliferative diseases. Methods -Rodent models of retinal neovascular disease including the mouse model of oxygen-induced retinopathy (OIR) and the very low density lipoprotein receptor (VLDLR) knockout (KO) mouse model were analyzed for Tspan/β-catenin regulation...
March 29, 2017: Circulation
https://www.readbyqxmd.com/read/28350091/clinical-implication-of-frizzled-2-expression-and-its-association-with-epithelial-to-mesenchymal-transition-in-hepatocellular-carcinoma
#20
Tomonari Asano, Suguru Yamada, Bryan C Fuchs, Hideki Takami, Masamichi Hayashi, Hiroyuki Sugimoto, Tsutomu Fujii, Kenneth K Tanabe, Yasuhiro Kodera
The epithelial-to-mesenchymal transition (EMT) is an initial, critical step in hepatocellular carcinoma (HCC) tumor invasion and metastasis. Frizzled 2 (Fzd2) expression might drive EMT through the non-canonical Wnt pathway, one of the various EMT signaling pathways. The expression of epithelial (E-cadherin) and mesenchymal (vimentin) markers, as well as that of Wnt5b, Stat3, IL-6, Jak2 and Fzd2, were measured in 15 HCC cell lines. The EMT status (vimentin to E-cadherin mRNA expression ratio), Fzd2 mRNA expression, and pSTAT3 protein expression were assessed by immunostaining in 100 HCC patients, and correlations of their expression with clinicopathological factors and prognosis were analyzed...
March 27, 2017: International Journal of Oncology
keyword
keyword
40692
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"