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https://www.readbyqxmd.com/read/28735480/immunophenotyping-by-multiparameter-flow-cytometry
#1
Weina Chen, Hung S Luu
Multiparameter flow cytometry has become an indispensable tool for the diagnosis and classification of acute myeloid leukemia (AML). The basic method relies on the unique ability to detect immunophenotypic abnormalities on discrete subpopulations. The primary roles in the initial assessment of AML are to determine the immaturity of the leukemic population, define the lineage and the immunophenotypic aberrancies in blasts, and identify characteristic immunophenotypic features to predict important recurrent cytogenetic and genetic abnormalities and prognosis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735474/heterogeneous-reaction-of-no2-with-soot-at-different-relative-humidity
#2
Chong Han, Yongchun Liu, Hong He
The influences of relative humidity (RH) on the heterogeneous reaction of NO2 with soot were investigated by a coated wall flow tube reactor at ambient pressure. The initial uptake coefficient (γ initial) of NO2 showed a significant decrease with increasing RH from 7 to 70%. The γ initial on "fuel-rich" and "fuel-lean" soot at RH = 7% was (2.59 ± 0.20) × 10(-5) and (5.92 ± 0.34) × 10(-6), respectively, and it decreased to (5.49 ± 0.83) × 10(-6) and (7.16 ± 0.73) × 10(-7) at RH = 70%, respectively...
July 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28735452/characterization-of-polymeric-substance-classes-in-cereal-based-beverages-using-asymmetrical-flow-field-flow-fractionation-with-a-multi-detection-system
#3
Georg Krebs, Thomas Becker, Martina Gastl
Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect different technological and especially sensory parameters of beverages. Asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle light scattering (MALS) and refractive index detection (dRI) or UV detection (UV) is a technique for structure and molar mass distribution analysis of macromolecules commonly used for pure compound solutions...
July 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28735438/adenosine-assisted-neurovascular-surgery-initial-case-series-and-review-of-literature
#4
REVIEW
Alaa Al-Mousa, Gahan Bose, Katharine Hunt, Ahmed K Toma
Cerebral aneurysms in complex anatomical locations and intraoperative rupture of aneurysms are challenging for neurosurgeons and anaesthetists alike. Mechanical and non-mechanical methods to reduce blood flow into aneurysms are well-recognised techniques to facilitate aneurysm exclusion from the circulation. Mechanical methods like temporary clipping of parent arteries, carotid artery ligation and endovascular balloon occlusion are commonly used in clinical practice. However, non-mechanical techniques such as rapid ventricular pacing and adenosine-induced cardiac standstill with hypotension are still emerging strategies...
July 22, 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/28735428/effects-of-low-intensity-pulsed-ultrasound-on-soft-tissue-micro-circulation-in-the-foot
#5
Anna Katharina Kösters, Bergita Ganse, Boyko Gueorguiev, Kajetan Klos, Ali Modabber, Sven Nebelung, Bong-Sung S Kim, Matthias Knobe
PURPOSE: Low-intensity pulsed ultrasound (LIPUS) has been shown to accelerate bone healing and is considered to increase blood flow. The aim of this study was to assess changes in micro-circulation of the foots' soft tissue in response to LIPUS intervention. We hypothesised improved micro-circulation in response to LIPUS. METHODS: Micro-circulation was assessed in 2 mm and 8 mm-deep skin of 50 healthy volunteers using non-invasive laser-doppler spectrophotometry (O2C-device)...
July 22, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28735358/il-10-and-tgf-%C3%AE-unbalanced-levels-in-neutrophils-contribute-to-increase-inflammatory-cytokine-expression-in-childhood-obesity
#6
Nayara I Medeiros, Rafael T Mattos, Carlos A Menezes, Rafaelle C G Fares, André Talvani, Walderez O Dutra, Fabrício Rios-Santos, Rodrigo Correa-Oliveira, Juliana A S Gomes
PURPOSE: Obesity is a multifactorial disease, associated with metabolic disorders, chronic low-grade inflammation, and impaired immunity. This study aimed to evaluate the childhood obesity-associated effects on neutrophil activation and cytokine production. METHODS: We evaluated activation and recognition markers and cytokine production in neutrophils from the peripheral blood of children with obesity and normal weight using multicolor flow cytometry. RESULTS: We demonstrate a higher frequency of neutrophils in childhood obesity group (CO) compared to normal-weight group (NW)...
July 22, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28735345/large-facial-lymphatic-malformation-treatment-using-sclerosing-agent-followed-by-surgical-resection-clinical-and-pathology-report
#7
Amir Laviv, Alexander Maly, Jawad Abu-Tair
Lymphatic malformations are rare slow-flow vascular malformations, with high tendency to appear in the head and neck region. The treatment of these lesions ranges from follow-up to sclerosing agent injection to surgical excision. The authors present a case of a new born with large extensive lingual and submandibular lymphatic malformation, for which the patient underwent tracheostomy and gastrostomy insertion. He was then treated successfully with sclerosing agent injections followed by surgical excision, with 7 years follow-up...
July 22, 2017: Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28735282/a-systematic-review-of-criteria-used-to-define-recovery-from-sport-related-concussion-in-youth-athletes
#8
REVIEW
Mohammad Nadir Haider, John J Leddy, Sonja Pavlesen, Melissa Kluczynski, John G Baker, Jeffrey C Miecznikowski, Barry S Willer
OBJECTIVE: The Concussion in Sport Group guidelines recommend a multifaceted approach to help clinicians make return to sport decisions. The purpose of this study was to identify the most common multifaceted measures used to define clinical recovery from sport-related concussion in young athletes (high school and/or college level) and to summarise existing knowledge of criteria used to make return to sport decisions. DESIGN: Systematic review. DATA SOURCES: The PubMed (MEDLINE), SPORTDiscus and Embase electronic databases were searched from 1 January 2000 to 1 March 2017 by three independent reviewers...
July 22, 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28735211/runoff-initiation-soil-detachment-and-connectivity-are-enhanced-as-a-consequence-of-vineyards-plantations
#9
A Cerdà, S D Keesstra, J Rodrigo-Comino, A Novara, P Pereira, E Brevik, A Giménez-Morera, M Fernández-Raga, M Pulido, S di Prima, A Jordán
Rainfall-induced soil erosion is a major threat, especially in agricultural soils. In the Mediterranean belt, vineyards are affected by high soil loss rates, leading to land degradation. Plantation of new vines is carried out after deep ploughing, use of heavy machinery, wheel traffic, and trampling. Those works result in soil physical properties changes and contribute to enhanced runoff rates and increased soil erosion rates. The objective of this paper is to assess the impact of the plantation of vineyards on soil hydrological and erosional response under low frequency - high magnitude rainfall events, the ones that under the Mediterranean climatic conditions trigger extreme soil erosion rates...
July 20, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28735209/seasonal-performance-of-denitrifying-bioreactors-in-the-northeastern-united-states-field-trials
#10
Bahareh Hassanpour, Shree Giri, William T Pluer, Tammo S Steenhuis, Larry D Geohring
Denitrifying bioreactors are increasingly being used for nitrate removal from agricultural drainage water. Filled with carbon substrates, often woodchips, denitrifying bioreactors provide a favorable anaerobic environment for denitrification. Despite performing well in loess soils in the Midwestern United States, field bioreactors have not yet been evaluated in shallow soils over glacial till that are characteristic for the Northeastern United States. This study, therefore, investigates the performance of bioreactors and provides design criteria for shallow soil with flashy discharges...
July 20, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28735182/fluidized-bed-fenton-coupled-with-ceramic-membrane-separation-for-advanced-treatment-of-flax-wastewater
#11
Dong Fan, Lili Ding, Hui Huang, Mengtian Chen, Hongqiang Ren
Fluidized-bed Fenton coupled with ceramic membrane separation to treat the flax secondary effluent was investigated. The operating variables, including initial pH, dosage of H2O2 and Fe(0), air flow rate, TMP and pore size, were optimized. The distributions of DOMs in the treatment process were analyzed. Under the optimum condition (600mgL(-1)H2O2, 1.4gL(-1) Fe(0), pH=3, 300Lh(-1) air flow rate and 15psi TMP), the highest TOC and color removal efficiencies were 84% and 94% in the coupled reactor with 100nm ceramic membrane, reducing 39% of total iron with similar removal efficiency compared with Fluidized-bed Fenton...
May 31, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28735156/adaptive-periodic-paralysis-allows-weaning-deep-sedation-overcoming-the-drowning-syndrome-in-ecmo-patients-bridged-for-lung-transplantation-a-case-series
#12
Irina Timofte, Michael Terrin, Erik Barr, June Kim, Joseph Rinaldi, Nicholas Ladikos, Jay Menaker, Ali Tabatabai, Zachary Kon, Bartley Griffith, Richard Pierson, Si Pham, Aldo Iacono, Daniel Herr
PURPOSE: Sedation in extracorporeal membrane oxygenation (ECMO) is challenging. Patients require deep sedation because of extremely high respiratory rates and increased work of breathing ("Drowning Syndrome") resulting in altered intra-thoracic pressure and reduced pump flow associated with hemodynamic compromise and decreased oxygenation. However, deep sedation impedes essential active rehabilitation with physical therapy. METHODS: We reviewed data on 3 ECMO patients for whom we used a novel approach to replace continuous drips with periodic sedation/paralysis...
July 17, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28735106/color-doppler-signals-of-blood-flow-in-the-corpus-luteum-and-vascular-perfusion-index-for-ovarian-and-uterine-arteries-during-expansion-of-the-allantochorion-in-bos-taurus-heifers
#13
F L V Pinaffi, E R Araujo, L A Silva, O J Ginther
Hemodynamics of the CL and each main uterine artery during expansion of the allantochorion from the ipsilateral side (CL side) to the contralateral side were studied in heifers (n = 8 nonbred, 9 pregnant). Progesterone concentration, vascular perfusion index for each uterine artery (by spectral ultrasonography), extent of blood flow in the CL (percentage of CL tissue with color-Doppler signals of blood flow), and intrauterine location of the expanding allantochorion (by gray-scale ultrasonic imaging) were determined daily from Days 14-60 (Day 0 = ovulation)...
July 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/28735076/quantifying-the-influence-of-oscillatory-flow-disturbances-on-blood-flow
#14
Sargon A Gabriel, Yan Ding, Yuqing Feng
Pulsatile blood flow is renowned for inducing localised flow disturbances that are characterised by significant oscillations. These flow disturbances are recognised to have a physiologic significance within the cardiovascular system, particularly with respect to their proatherogenic expression on endothelial cells. Flow disturbances also impart significant influence on the mechanics of cardiovascular flow and are formally shown in the present study to be coupled to the period-average behaviour of a pulsatile flow field, causing it to be misrepresented by its steady-equivalent; which is often used in models of atherogenesis and hence limits their reliability...
July 19, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28735065/time-required-for-resetting-postural-effects-on-serum-constituents-in-healthy-individuals
#15
Yoshihisa Shimizu, Kiyoshi Ichihara, Katsunori Kouguchi
OBJECTIVE: We investigated postural effects on concentrations of commonly measured serum analytes and sought for optimal duration of sitting before venipuncture in an outpatient setting. METHODS: Seven healthy volunteers changed posture serially at 30-minute (min) intervals from lying, sitting, slowly walking, sitting, and to lying again. At the end of the first lying posture, wing-needle was inserted into cubital vein and 3mL of blood was drawn every 5min. All the serum was stored at -80°C until the time of collective measurements for 16 major chemistry analytes two weeks later...
July 19, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28735019/1-year-surgical-outcomes-of-complete-or-incomplete-enucleation-of-prostate-by-monopolar-electrocoagulation-photoselective-vapoenucleation-of-120w-greenlight-laser-and-holmium-laser
#16
Sung Yong Cho, Juhyun Park, Sangjun You, Min Chul Cho, Hyeon Jeong, Hwancheol Son
OBJECTIVE: To evaluate surgical outcomes of patients who underwent complete or incomplete enucleation technique during a short-term postoperative period METHODS: Patients having intractable LUTS/BPH and prostates > 30 grams with obstructed pattern in the urodynamic examinations were included. They underwent TUR-P, 120 W greenlight laser HPS-PVEP, or HoLEP. Patients with the size of remnant prostates minus surgical defects < 25 grams were grouped into the completely enucleated group (group C) and other were group into the partially enucleated group (group P)...
July 19, 2017: Urology
https://www.readbyqxmd.com/read/28734962/analyzing-the-hepatoprotective-effect-of-the-swertia-cincta-burkillextract-against-anit-induced-cholestasis-in-rats-by-modulating-the-expression-of-transporters-and-metabolic-enzymes
#17
Lili Xu, Tingting Sheng, Xiaolong Liu, Tong Zhang, Zhengtao Wang, Han Han
ETHNOPHARMACOLOGICAL RELEVANCE: Swertia cincta Burkill was traditionally used for treating jaundice and various types of chronic and acute hepatitis in Yunnan and Tibet in China for hundreds of years. This study aims to investigate the protective effect of S. cincta Burkill (ESC) extract on alpha-naphthylisothiocyanate (ANIT)-induced hepatotoxicity and cholestasis in rats. MATERIALS AND METHODS: Crude extracts were prepared using 90% ethanol and by vacuum drying...
July 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28734933/photosystem-ii-cyclic-electron-flow-powers-exceptional-photoprotection-and-record-growth-in-the-microalga-chlorella-ohadii
#18
Gennady Ananyev, Colin Gates, Aaron Kaplan, G Charles Dismukes
The desert microalga Chlorella ohadii was reported to grow at extreme light intensities with minimal photoinhibition, tolerate frequent de/re-hydrations, yet minimally employs antenna-based non-photochemical quenching for photoprotection. Here we investigate the molecular mechanisms by measuring Photosystem II charge separation yield (chlorophyll variable fluorescence, Fv/Fm) and flash-induced O2 yield to measure the contributions from both linear (PSII-LEF) and cyclic (PSII-CEF) electron flow within PSII. Cells grow increasingly faster at higher light intensities (μE/m(2)/s) from low (20) to high (200) to extreme (2000) by escalating photoprotection via shifting from PSII-LEF to PSII-CEF...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734873/a-computational-fluid-structure-interaction-analysis-of-coronary-y-grafts
#19
Bruno Guerciotti, Christian Vergara, Sonia Ippolito, Alfio Quarteroni, Carlo Antona, Roberto Scrofani
Coronary artery disease is one of the leading causes of death worldwide. The stenotic coronary vessels are generally treated with coronary artery bypass grafts (CABGs), which can be either arterial (internal mammary artery, radial artery) or venous (saphenous vein). However, the different mechanical properties of the graft can influence the outcome of the procedure in terms of risk of restenosis and subsequent graft failure. In this paper, we perform a computational fluid-structure interaction (FSI) analysis of patient-specific multiple CABGs (Y-grafts) with the aim of better understanding the influence of the choice of bypass (arterial vs venous) on the risk of graft failure...
July 19, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28734872/modeling-the-cleavage-of-von-willebrand-factor-by-adamts13-protease-in-shear-flow
#20
Brooke Huisman, Masoud Hoore, Gerhard Gompper, Dmitry A Fedosov
Von Willebrand factor (VWF) is a key protein in hemostasis as it mediates adhesion of blood platelets to a site of vascular injury. A proper distribution of VWF lengths is important for normal functioning of hemostatic processes, because a diminished number of long VWF chains may significantly limit blood clotting and lead to bleeding, while an abundant number of long VWFs may result in undesired thrombotic events. VWF size distribution is controlled by ADAMTS13 protease, which can cleave VWF chains beyond a critical shear rate when the chains are stretched enough such that cleavage sites become accessible...
July 19, 2017: Medical Engineering & Physics
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