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https://www.readbyqxmd.com/read/28941453/development-of-a-dna-aptamer-for-screening-neisseria-meningitidis-serogroup-b-by-cell-selex
#1
Kimia Mirzakhani, Seyed Latif Mousavi Gargari, Iraj Rasooli, Samaneh Rasoulinejad
Background: Artificial oligonucleotides like DNA or RNA aptamers can be used as biodiagnostic alternatives for antibodies to detect pathogens. Comparing to antibodies, artificial oligonucleotides are produced easily at lower costs and are more stable. Neisseria meningitidis, the causative agent of meningitis, is responsible for about 1% of infections in an epidemic period. Specific DNA aptamers that bind to N. meningitidis serogroup B were identified by whole-cell Systemic Evolution of Ligands by EXponential Enrichment (SELEX)...
September 24, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28941416/increased-cycles-of-dc-cik-immunotherapy-decreases-frequency-of-tregs-in-patients-with-resected-nsclc
#2
Haiping Song, Shujuan Liu, Ziyun Zhao, Weihong Sun, Xiaofang Wei, Xuezhen Ma, Peng Zhao, Daiqing Gao
Regulatory T cells (Tregs) suppress antitumor immune responses. Cycles of Dendritic cells (DC) vaccination combined with cytokine-induced killer (CIK) cells (DC/CIK) treatment were significantly related with good prognosis. Therefore, we investigated whether increased cycles of immunotherapy could decrease frequency of Tregs in patients with resected non-small cell lung cancer (NSCLC). Previous study from our laboratory has determined that the optimal cutoff point of the cycle count was 3cycles. We examined the levels of Tregs and the related cytokines by flow cytometric and cytokine analysis in these patients after more than (≥) 3cycles or less than (<) 3cycles of DC/CIK cell treatment...
September 20, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28938788/cd4-t-cells-dominate-the-leukocyte-infiltration-response-initiated-by-intra-dermal-injection-of-phytohemagglutinin-into-growing-feathers-in-chickens
#3
K S Sullivan, G F Erf
Phytohemagglutinin (PHA) is commonly used to evaluate cell-mediated immunocompetence. In chickens, PHA is typically injected intra-dermally (i.d.) into the skin (e.g., wing web, wattle, or footpad), and the tissue swelling response is monitored, whereby the extent of tissue swelling positively relates to the individual's cell-mediated immune system capabilities. Although i.d. injected PHA was shown to stimulate mononuclear cell and basophil infiltration to the site of injection, reports on temporal, qualitative, and quantitative aspects of the local cutaneous PHA response are limited...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938771/comparative-estimation-of-inevitable-endogenous-ileal-flow-of-amino-acids-in-pekin-ducks-under-varying-dietary-or-physiological-conditions-and-their-significance-to-nutritional-requirements-for-amino-acids
#4
D O Akinde
In 2 experiments in Pekin ducks the inevitable endogenous ileal flow (IEIF) of AA was estimated at changing intake and source of crude fiber (CF) or soybean oil (SO) level. Also the roles of dry matter intake (DMI) and BW or age as well as the proportion of IEIF in the dietary requirement for AA were studied. In experiment 1 three basal CP (20, 60, or 100 g/kg) diets were formulated containing a low CF (LCF, 30 g/kg) or high (HCF, 80 g/kg) level; achieved with cellulose supplementation. All diets were similar in every other respect including having SO content of 40 g/kg...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938750/allowable-warm-ischemic-time-and-morphological-and-biochemical-changes-in-uterine-ischemia-reperfusion-injury-in-cynomolgus-macaque-a-basic-study-for-uterus-transplantation
#5
Iori Kisu, Kiyoko Umeneo, Masataka Adachi, Katsura Emoto, Yuya Nogami, Kouji Banno, Iori Itagaki, Ikuo Kawamoto, Takahiro Nakagawa, Hayato Narita, Atsushi Yoshida, Hideaki Tsuchiya, Kazumasa Ogasawara, Daisuke Aoki
STUDY QUESTION: How long is the allowable warm ischemic time of the uterus and what morphological and biochemical changes are caused by uterine ischemia/reperfusion injury in cynomolgus macaques? SUMMARY ANSWER: Warm ischemia in the uterus of cynomolgus macaques is tolerated for up to 4 h and reperfusion after uterine ischemia caused no further morphological and biochemical changes. WHAT IS KNOWN ALREADY: Uterus transplantation is a potential option for women with uterine factor infertility...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938593/endoplasmic-reticulum-stress-mediated-membrane-expression-of-crt-erp57-induces-immunogenic-apoptosis-in-drug-resistant-endometrial-cancer-cells
#6
Qin Xu, Chuanben Chen, An Lin, Yunqing Xie
OBJECTIVE: To investigate the role of endoplasmic reticulum (ER) stress-mediated CRT/ERp57 complex expression underlying the mechanism of resistance to doxorubicin (DOX) in endometrial carcinoma (EC) in vivo and in vitro. METHODS: The expression of CRT, ERp57, p-PERK, eIF2α, p-eIF2α in EC patients and EC cells was detected by Western blots and by immunofluorescence assay. MTT assay was used to determine the LC50 of EC cells to DOX and cell viability. Apoptosis was assayed using flow cytometer...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938496/icaritin-improves-antibody-induced-thrombocytopenia-in-a-mouse-model-by-regulating-t-cell-polarization
#7
Chenghong Sun, Jian Yang, Mingzhi Wang, Lihong Pan, Jingchun Yao, Shenglan Wang, Na Guo, Chunyan Li, Guimin Zhang
Previous studies have shown that icaritin (ICT) has significant protective effects on immune thrombocytopenia (ITP), and the present study aimed to discuss the mechanism of this protective effect from the aspect of regulating T-cell polarization by an antibody-induced ITP mice model. Mice were given rat anti-mouse CD41 antibody (MWReg30) by intraperitoneal injection for 7 d to produce ITP model. At the same time, ICT was administrated at 10 mg/kg/d orally for 9 d. Peripheral blood platelets were counted by hematology analyzer...
September 22, 2017: Planta Medica
https://www.readbyqxmd.com/read/28938435/the-role-of-sequential-bmp-signaling-in-directing-human-embryonic-stem-cells-to-bipotential-gonadal-cells
#8
Kirsi Sepponen, Karolina Lundin, Katri Knuus, Pia Väyrynen, Taneli Raivio, Juha S Tapanainen, Timo Tuuri
Context: Human gonads arise as a pair of epithelial ridges on the surface of intermediate mesoderm (IM)-derived mesonephros. Toxic environmental factors and mutations in various genes are known to disturb normal gonadal development, but due to a lack of suitable in vitro models, detailed studies characterizing the molecular basis of the observed defects have not been performed. Objective: Our objective was to establish an in vitro method for studying differentiation of bipotential gonadal progenitors using human embryonic stem cells (hESCs), and to investigate the role of bone morphogenetic protein (BMP) in gonadal differentiation...
August 29, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938397/regulation-of-orbital-fibrosis-and-adipogenesis-by-pathogenic-th17-cells-in-graves-orbitopathy
#9
Sijie Fang, Yazhuo Huang, Sisi Zhong, Yangyang Li, Yidan Zhang, Yinwei Li, Jing Sun, Xingtong Liu, Yang Wang, Shuo Zhang, Tianle Xu, Xiaodong Sun, Ping Gu, Dan Li, Huifang Zhou, Bin Li, Xianqun Fan
Context: Th17 cells are correlated with many human autoimmune disorders including Graves' disease, and may play key roles in the pathogenesis of Graves' orbitopathy (GO). Objective: To study the phenotype of Th17 cells in patients with GO and healthy subjects, investigate the fibrosis and adipogenesis in orbital fibroblasts (OFs) modulated by IL-17A, and determine the interaction between Th17 cells and OFs. Design/Setting/Participants: Blood samples and orbital tissues from GO patients and healthy controls were collected...
August 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28938308/sensor-based-physiologic-control-strategy-for-biventricular-support-with-rotary-blood-pumps
#10
Yu Wang, Steven C Koenig, Zhongjun Wu, Mark S Slaughter, Guruprasad A Giridharan
Rotary biventricular assist devices (BiVAD) are becoming a clinically accepted treatment option for end-stage biventricular failure. To improve BiVAD efficacy and safety, we propose a control algorithm to achieve the clinical objectives of maintaining left-right-sided balance, restoring physiologic flows, and preventing ventricular suction. The control algorithm consists of two proportional-integral (PI) controllers for left and right ventricular assist devices (LVAD and RVAD) to maintain differential pump pressure across LVAD (ΔPL) and RVAD (ΔPR) to provide left-right balance and physiologic flow...
September 21, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28938301/are-we-fully-utilizing-the-functionalities-of-modern-operating-room-ventilators
#11
Shujie Liu, Robert M Kacmarek, Jun Oto
PURPOSE OF REVIEW: The modern operating room ventilators have become very sophisticated and many of their features are comparable with those of an ICU ventilator. To fully utilize the functionality of modern operating room ventilators, it is important for clinicians to understand in depth the working principle of these ventilators and their functionalities. RECENT FINDINGS: Piston ventilators have the advantages of delivering accurate tidal volume and certain flow compensation functions...
September 21, 2017: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/28938215/evaluation-of-bioenergy-crop-growth-and-the-impacts-of-bioenergy-crops-on-streamflow-tile-drain-flow-and-nutrient-losses-in-an-extensively-tile-drained-watershed-using-swat
#12
Tian Guo, Raj Cibin, Indrajeet Chaubey, Margaret Gitau, Jeffrey G Arnold, Raghavan Srinivasan, James R Kiniry, Bernard A Engel
Large quantities of biofuel production are expected from bioenergy crops at a national scale to meet US biofuel goals. It is important to study biomass production of bioenergy crops and the impacts of these crops on water quantity and quality to identify environment-friendly and productive biofeedstock systems. SWAT2012 with a new tile drainage routine and improved perennial grass and tree growth simulation was used to model long-term annual biomass yields, streamflow, tile flow, sediment load, and nutrient losses under various bioenergy scenarios in an extensively agricultural watershed in the Midwestern US...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28938213/combination-of-aquifer-thermal-energy-storage-and-enhanced-bioremediation-biological-and-chemical-clogging
#13
Zhuobiao Ni, Pauline van Gaans, Huub Rijnaarts, Tim Grotenhuis
Interest in the combination concept of aquifer thermal energy storage (ATES) and enhanced bioremediation has recently risen due to the demand for both renewable energy technology and sustainable groundwater management in urban areas. However, the impact of enhanced bioremediation on ATES is not yet clear. Of main concern is the potential for biological clogging which might be enhanced and hamper the proper functioning of ATES. On the other hand, more reduced conditions in the subsurface by enhanced bioremediation might lower the chance of chemical clogging, which is normally caused by Fe(III) precipitate...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28938157/adsorption-combined-with-superconducting-high-gradient-magnetic-separation-technique-used-for-removal-of-arsenic-and-antimony
#14
Zenglu Qi, Tista Prasai Joshi, Ruiping Liu, Yiran Li, Huijuan Liu, Jiuhui Qu
Manganese iron oxide (MnFe2O4), an excellent arsenic(As)/antimony(Sb) removal adsorbent, is greatly restricted for the solid-liquid separation. Through the application of superconducting high gradient magnetic separation (HGMS) technique, we herein constructed a facility for the in situ solid-liquid separation of micro-sized MnFe2O4 adsorbent in As/Sb removal process. To the relative low initial concentration 50.0μgL(-1), MnFe2O4 material sorbent can still decrease As or Sb below US EPA's drinking water standard limit...
September 7, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28938123/reconstructing-prehistoric-african-population-structure
#15
Pontus Skoglund, Jessica C Thompson, Mary E Prendergast, Alissa Mittnik, Kendra Sirak, Mateja Hajdinjak, Tasneem Salie, Nadin Rohland, Swapan Mallick, Alexander Peltzer, Anja Heinze, Iñigo Olalde, Matthew Ferry, Eadaoin Harney, Megan Michel, Kristin Stewardson, Jessica I Cerezo-Román, Chrissy Chiumia, Alison Crowther, Elizabeth Gomani-Chindebvu, Agness O Gidna, Katherine M Grillo, I Taneli Helenius, Garrett Hellenthal, Richard Helm, Mark Horton, Saioa López, Audax Z P Mabulla, John Parkington, Ceri Shipton, Mark G Thomas, Ruth Tibesasa, Menno Welling, Vanessa M Hayes, Douglas J Kennett, Raj Ramesar, Matthias Meyer, Svante Pääbo, Nick Patterson, Alan G Morris, Nicole Boivin, Ron Pinhasi, Johannes Krause, David Reich
We assembled genome-wide data from 16 prehistoric Africans. We show that the anciently divergent lineage that comprises the primary ancestry of the southern African San had a wider distribution in the past, contributing approximately two-thirds of the ancestry of Malawi hunter-gatherers ∼8,100-2,500 years ago and approximately one-third of the ancestry of Tanzanian hunter-gatherers ∼1,400 years ago. We document how the spread of farmers from western Africa involved complete replacement of local hunter-gatherers in some regions, and we track the spread of herders by showing that the population of a ∼3,100-year-old pastoralist from Tanzania contributed ancestry to people from northeastern to southern Africa, including a ∼1,200-year-old southern African pastoralist...
September 21, 2017: Cell
https://www.readbyqxmd.com/read/28938092/ctcf-mediated-enhancer-promoter-interaction-is-a-critical-regulator-of-cell-to-cell-variation-of-gene-expression
#16
Gang Ren, Wenfei Jin, Kairong Cui, Joseph Rodrigez, Gangqing Hu, Zhiying Zhang, Daniel R Larson, Keji Zhao
Recent studies indicate that even a homogeneous population of cells display heterogeneity in gene expression and response to environmental stimuli. Although promoter structure critically influences the cell-to-cell variation of gene expression in bacteria and lower eukaryotes, it remains unclear what controls the gene expression noise in mammals. Here we report that CTCF decreases cell-to-cell variation of expression by stabilizing enhancer-promoter interaction. We show that CTCF binding sites are interwoven with enhancers within topologically associated domains (TADs) and a positive correlation is found between CTCF binding and the activity of the associated enhancers...
September 21, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28938010/epidermal-growth-factor-receptor-targeted-sonoporation-with-microbubbles-enhances-therapeutic-efficacy-in-a-squamous-cell-carcinoma-model
#17
Fumika Hirabayashi, Kenjiro Iwanaga, Toshinori Okinaga, Osamu Takahashi, Wataru Ariyoshi, Ryo Suzuki, Mutsumi Sugii, Kazuo Maruyama, Kazuhiro Tominaga, Tatsuji Nishihara
Sonoporation is a drug and gene delivery system using ultrasonication that allows the intracellular delivery of foreign molecules that cannot enter cells under normal conditions. We previously reported that sonoporation with microbubbles (MBs) could achieve effective intracellular drug delivery to human gingival squamous carcinoma Ca9-22 cells. In this study, we developed anti-epidermal growth factor receptor (EGFR) antibody-conjugated MBs (EGFR-MBs) and evaluated their capacity to enhance anti-cancer drug toxicity in vitro and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28937927/the-effects-of-photobiomodulation-delivered-by-light-emitting-diode-on-stem-cells-from-human-exfoliated-deciduous-teeth-a-study-on-the-relevance-to-pluripotent-stem-cell-viability-and-proliferation
#18
Katia Llanos do Vale, Durvanei Augusto Maria, Lara Cristina Picoli, Alessandro Melo Deana, Marcelo Betti Mascaro, Raquel Agnelli Mesquita Ferrari, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes
OBJECTIVE: Photobiomodulation (PBM) can modulate the proliferation of some types of stem cells. However, few reports have addressed the effects of PBM delivered by light-emitting diode (LED) on stem cells obtained from the pulp tissue of deciduous teeth. The aim of the present study was to investigate the effect of PBM delivered by red LED (630 nm, 75 mW, 37 mW/cm(2)) with different radiant exposures on the cell cycle, mitochondrial membrane potential, and senescence of stem cells from human exfoliated deciduous teeth (SHED)...
September 21, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28937857/evaluation-of-retinal-blood-flow-before-and-after-panretinal-photocoagulation-using-pattern-scan-laser-for-diabetic-retinopathy
#19
Yoshihisa Yamada, Kiyoshi Suzuma, Naoko Onizuka, Masafumi Uematsu, Yasser Helmy Mohamed, Takashi Kitaoka
PURPOSE: Laser speckle flowgraphy (LSFG) can measure blood flow in the ocular fundus. We analyzed the relationship between retinal blood flow and panretinal photocoagulation (PRP) in diabetic retinopathy. METHODS: This retrospective observational study examined the eyes of 35 patients with proliferative diabetic retinopathy (PDR) or non-PDR (NPDR). PRP was performed using a pattern scan laser. Using LSFG, blood flow was evaluated as the mean blur rate (MBR) or the relative flow volume (RFV)...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937811/maladaptive-plasticity-masks-the-effects-of-natural-selection-in-the-red-shouldered-soapberry-bug
#20
Meredith L Cenzer
Natural selection can produce local adaptation, but local adaptation can be masked by maladaptive plasticity. Maladaptive plasticity may arise as a result of gene flow producing novel gene combinations that have not been exposed to selection. In the 1980s, populations of the red-shouldered soapberry bug (Jadera haematoloma) were locally adapted to feed on the seeds of a native host plant and an introduced host plant; by 2014, local differentiation in beak length had been lost, likely as a consequence of increased gene flow...
October 2017: American Naturalist
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