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https://www.readbyqxmd.com/read/29156626/surface-plasmon-resonance-sensing-of-biorecognition-interactions-within-the-tumor-suppressor-p53-network
#1
REVIEW
Ilaria Moscetti, Salvatore Cannistraro, Anna Rita Bizzarri
Surface Plasmon Resonance (SPR) is a powerful technique to study the kinetics of biomolecules undergoing biorecognition processes, particularly suited for protein-protein interactions of biomedical interest. The potentiality of SPR was exploited to sense the interactions occurring within the network of the tumor suppressor p53, which is crucial for maintaining genome integrity and whose function is inactivated, mainly by down regulation or by mutation, in the majority of human tumors. This study includes p53 down-regulators, p53 mutants and also the p53 family members, p63 and p73, which could vicariate p53 protective function...
November 20, 2017: Sensors
https://www.readbyqxmd.com/read/29156134/organic-ice-resists
#2
William Tiddi, Anna Elsukova, Hoa Thanh Le, Pei Liu, Marco Beleggia, Anpan Han
Electron-beam lithography (EBL) is the backbone technology for patterning nanostructures and manufacturing nanodevices. It involves processing and handling synthetic resins in several steps, each requiring optimization and dedicated instrumentation in cleanroom environments. Here, we show that simple organic molecules, e.g. alcohols, condensed to form thin-films at low temperature demonstrate resist-like capabilities for EBL applications and beyond. The entire lithographic process takes place in a single instrument, and avoids exposing chemicals to the user and the need of cleanrooms...
November 20, 2017: Nano Letters
https://www.readbyqxmd.com/read/29155906/a-combined-continuous-wave-electron-paramagnetic-resonance-and-dft-calculations-of-copper-doped-cd0-98cu0-02-prz-trz-ia-metal-organic-framework
#3
Anastasia Kultaeva, Timur Biktagirov, Jens Bergmann, Linda Hensel, Harald Krautscheid, Andreas Pöppl
Continuous wave X-band electron paramagnetic resonance (EPR) and density functional theory (DFT) were successfully applied to explore the incorporation and coordination state of the Cu(2+) ions in the [Cd0.98Cu0.02(prz-trz-ia)] porous metal-organic frameworks. EPR measurements on powder samples and single crystals provided the full electron Zeeman g and copper hyperfine A(Cu) interaction tensors including the orientation of their principal axes frames. DFT computations allowed for a detailed interpretation of the experimental results in terms of coordination symmetry and binding properties of the paramagnetic Cu(2+) ions...
November 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29155710/dna-nanotubes-as-a-versatile-tool-to-study-semiflexible-polymers
#4
Jörg Schnauß, Martin Glaser, Jessica S Lorenz, Carsten Schuldt, Christin Möser, Martin Sajfutdinow, Tina Händler, Josef A Käs, David M Smith
Mechanical properties of complex, polymer-based soft matter, such as cells or biopolymer networks, can be understood in neither the classical frame of flexible polymers nor of rigid rods. Underlying filaments remain outstretched due to their non-vanishing backbone stiffness, which is quantified via the persistence length (lp), but they are also subject to strong thermal fluctuations. Their finite bending stiffness leads to unique, non-trivial collective mechanics of bulk networks, enabling the formation of stable scaffolds at low volume fractions while providing large mesh sizes...
October 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155591/mechanics-of-protein-adaptation-to-high-temperatures
#5
Guillaume Stirnemann, Fabio Sterpone
Inspired by Somero's corresponding state principle that relates protein enhanced thermal stability with mechanical rigidity, we deployed state of the art computational techniques (based on atomistic steered molecular dynamics and Hamiltonian-replica exchange simulations) to study the in silico realization of mechanical and thermal unfolding of two homologous Csp proteins that have evolved to thrive in different thermal environments. By complementing recent single-molecule experiments, we unambiguously show that, for these homologues whose structures are very similar, the increased thermal resistance of the thermophilic variant is not associated with an increased mechanical stability...
November 20, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29155564/mass-spectrometry-imaging-a-review-of-emerging-advancements-and-future-insights
#6
Amanda R Buchberger, Kellen DeLaney, Jillian Johnson, Lingjun Li
Mass spectrometry imaging (MSI) is a powerful tool that enables untargeted investigations into the spatial distribution of molecular species in a variety of samples. It has the capability to image thousands of molecules, such as metabolites, lipids, peptides, proteins, and glycans, in a single experiment without labeling. The combination of information gained from mass spectrometry (MS) and visualization of spatial distributions in thin sample sections makes this a valuable chemical analysis tool useful for biological specimen characterization...
November 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29155467/synthesis-characterisation-and-reactions-of-truly-cationic-ni-i-phosphine-complexes
#7
Ingo Krossing, Miriam Mareen Schwab, Daniel Himmel, Sylwia Kacprzak, Valentin Radtke, Daniel Kratzert, Philippe Weis, Melanie Wernet, Andreas Peter, Zeinab Yassine, Dominik Schmitz, Ernst-Wilhelm Scheidt, Wolfgang Scherer, Stefan Weber, Wolfram Feuerstein, Frank Breher, Alexander Higelin
The recently published purely metalorganic NiI salt [Ni(cod)2][Al(ORF)4] (1, cod = 1,5-cyclooctadiene, RF = C(CF3)3) provides a starting point for a new synthesis strategy leading to NiI phosphine complexes, replacing cod ligands by phosphines. Clearly visible colour changes indicate reactions within minutes, while quantum chemical calculations (PBE0-D3(BJ)/def2-TZVPP) approve exergonic reaction enthalpies in all performed ligand exchange reactions. Hence, [Ni(dppp)2][Al(ORF)4] (2, dppp = 1,3-Bis(diphenylphosphino)propane), [Ni(dppe)2][Al(ORF)4] (3, dppe = 1,3-Bis(diphenyl-phosphino)ethane), three-coordinate [Ni(PPh3)3][Al(ORF)4] (4) and a remarkable two-coordinate NiI phosphine complex [Ni(PtBu3)2][Al(ORF)4] (5) were characterised via single crystal X-ray structure analysis...
November 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29155132/stiffening-of-flexible-sumo1-protein-upon-peptide-binding-analysis-with-anisotropic-network-model
#8
Ranja Sarkar
SUMO (small ubiquitin-like modifier) proteins interact with a large number of target proteins via a key regulatory event called sumoylation that encompasses activation, conjugation and ligation of SUMO proteins through specific E1, E2, and E3-type enzymes respectively. Single-molecule atomic force microscopic (AFM) experiments performed to unravel bound SUMO1 along its N-C termini direction reveal that E3-ligases (in the form of small peptides) increase mechanical stability (along the axis) of the flexible protein upon binding...
November 16, 2017: Mathematical Biosciences
https://www.readbyqxmd.com/read/29153839/asymmetry-between-activation-and-deactivation-during-a-transcriptional-pulse
#9
Lee S S Dunham, Hiroshi Momiji, Claire V Harper, Polly J Downton, Kirsty Hey, Anne McNamara, Karen Featherstone, David G Spiller, David A Rand, Bärbel Finkenstädt, Michael R H White, Julian R E Davis
Transcription in eukaryotic cells occurs in gene-specific bursts or pulses of activity. Recent studies identified a spectrum of transcriptionally active "on-states," interspersed with periods of inactivity, but these "off-states" and the process of transcriptional deactivation are poorly understood. To examine what occurs during deactivation, we investigate the dynamics of switching between variable rates. We measured live single-cell expression of luciferase reporters from human growth hormone or human prolactin promoters in a pituitary cell line...
November 13, 2017: Cell Systems
https://www.readbyqxmd.com/read/29153094/differences-between-chronic-lymphocytic-leukaemia-and-small-lymphocytic-lymphoma-cells-by-proteomic-profiling-and-snp-microarray-analysis
#10
Jennifer A Tooze, Edita Hamzic, Fenella Willis, Ruth Pettengell
The majority of malignant cells in chronic lymphocytic leukaemia (CLL) circulate in the peripheral blood whereas small lymphocytic lymphoma (SLL) cells reside in tissues. The aim of this study was to detect differences in chemokine receptor expression, DNA single nucleotide polymorphism (SNP) microarray analysis and proteomic profiling to help elucidate why the cells remain in their respective environments. We identified by flow cytometric studies of chemokine receptors and DNA SNP microarray analysis significant differences between cells from CLL and SLL patients...
December 2017: Cancer Genetics
https://www.readbyqxmd.com/read/29153085/an-ultra-sensitive-fluorescent-turn-on-biosensor-for-glutathione-and-its-application-in-living-cells
#11
Wei Wang, Xiaoshan Hou, Xin Li, Chen Chen, Xiliang Luo
In this work, an effective controlled-release biosensor based on Au nanocages (AuNCs) capped with disulfide-containing DNA molecular gates was developed for ultra-sensitive and highly selective detection of glutathione (GSH). Oligonucleotides containing the S-S bonds were assembled on the surface of the AuNCs by means of electrostatic interactions in order to inhibit the release of fluorescent molecules such as Rhodamine B (RhB) loaded by AuNCs. In the presence of GSH, due to the specific cleavage of S-S bonds in disulfide-containing single-stranded DNAs (ssDNAs) as well as their subsequent departure from the surface of AuNCs, the pores could be opened, and then the dye molecules would be released from AuNCs...
January 15, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29152567/scanning-wave-photopolymerization-enables-dye-free-alignment-patterning-of-liquid-crystals
#12
Kyohei Hisano, Miho Aizawa, Masaki Ishizu, Yosuke Kurata, Wataru Nakano, Norihisa Akamatsu, Christopher J Barrett, Atsushi Shishido
Hierarchical control of two-dimensional (2D) molecular alignment patterns over large areas is essential for designing high-functional organic materials and devices. However, even by the most powerful current methods, dye molecules that discolor and destabilize the materials need to be doped in, complicating the process. We present a dye-free alignment patterning technique, based on a scanning wave photopolymerization (SWaP) concept, that achieves a spatial light-triggered mass flow to direct molecular order using scanning light to propagate the wavefront...
November 2017: Science Advances
https://www.readbyqxmd.com/read/29151793/plasma-protein-absolute-quantification-by-nano-lc-q-tof-udms-e-for-clinical-biomarker-verification
#13
Maria Ilies, Cristina Adela Iuga, Felicia Loghin, Vishnu Mukund Dhople, Elke Hammer
Background and aims: Proteome-based biomarker studies are targeting proteins that could serve as diagnostic, prognosis, and prediction molecules. In the clinical routine, immunoassays are currently used for the absolute quantification of such biomarkers, with the major limitation that only one molecule can be targeted per assay. The aim of our study was to test a mass spectrometry based absolute quantification method for the verification of plasma protein sets which might serve as reliable biomarker panels for the clinical practice...
2017: Clujul Medical (1957)
https://www.readbyqxmd.com/read/29151676/jim-hyde-and-the-endor-connection-a-personal-account
#14
Klaus Möbius, Wolfgang Lubitz, Anton Savitsky
In this minireview, we report on our year-long EPR work, such as electron-nuclear double resonance (ENDOR), pulse electron double resonance (PELDOR) and ELDOR-detected NMR (EDNMR) at X-band and W-band microwave frequencies and magnetic fields. This report is dedicated to James S. Hyde and honors his pioneering contributions to the measurement of spin interactions in large (bio)molecules. From these interactions, detailed information is revealed on structure and dynamics of macromolecules embedded in liquid-solution or solid-state environments...
2017: Applied Magnetic Resonance
https://www.readbyqxmd.com/read/29151636/bedaquiline-fallible-hope-against-drug-resistant-tuberculosis
#15
REVIEW
Priya Singh, Rashmi Kumari, Rup Lal
Tuberculosis (TB) is a deadly bacterial infectious disease caused by intra-cellular pathogen Mycobacterium tuberculosis (Mtb). There were an estimated 1.4 million TB deaths in 2015 and an additional 0.4 million deaths resulting from TB among individuals with HIV. Drug-discovery for its cure is very slow in comparison with the causative organism's fast pace of mutations conferring drug resistance. Moreover, the field of drug-discovery of anti-TB drugs is constantly being challenged by the drug resistant strains of Mtb...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29150961/modulation-of-igg1-immunoeffector-function-by-glycoengineering-of-the-gdp-fucose-biosynthesis-pathway
#16
Ronan M Kelly, Ronald L Kowle, Zhirui Lian, Beth A Strifler, Derrick R Witcher, Bhavin S Parekh, Tongtong Wang, Christopher C Frye
Cross-linking of the Fcγ receptors expressed on the surface of hematopoietic cells by IgG immune complexes triggers the activation of key immune effector mechanisms, including antibody-dependent cell mediated cytotoxicity (ADCC). A conserved N-glycan positioned at the N-terminal region of the IgG CH 2 domain is critical in maintaining the quaternary structure of the molecule for Fcγ receptor engagement. The removal of a single core fucose residue from the N-glycan results in a considerable increase in affinity for FcγRIIIa leading to an enhanced receptor-mediated immunoeffector function...
November 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29150907/sensitivity-of-doping-biomarkers-after-administration-of-a-single-dose-testosterone-gel
#17
Jenny Mullen, Annica Börjesson, Oscar Hopcraft, Jenny J Schulze, Magnus Ericsson, Anders Rane, Mikael Lehtihet, Lena Ekström
Micro-doping with testosterone (T) is challenging to detect with the current doping tests. Today, the methods available to detect T are longitudinally monitoring of urine biomarkers in the Athlete Biological Passport (ABP) and measuring the isotopic composition of excreted biomarkers to distinguish the origin of the molecule. In this study, we investigated the detectability of a single dose of 100 mg T gel in 8 healthy male subjects. We also studied which biomarkers were most sensitive to T gel administration, including blood biomarkers...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150596/forming-a-three-dimensional-porous-organic-network-via-solid-state-explosion-of-organic-single-crystals
#18
Seo-Yoon Bae, Dongwook Kim, Dongbin Shin, Javeed Mahmood, In-Yup Jeon, Sun-Min Jung, Sun-Hee Shin, Seok-Jin Kim, Noejung Park, Myoung Soo Lah, Jong-Beom Baek
Solid-state reaction of organic molecules holds a considerable advantage over liquid-phase processes in the manufacturing industry. However, the research progress in exploring this benefit is largely staggering, which leaves few liquid-phase systems to work with. Here, we show a synthetic protocol for the formation of a three-dimensional porous organic network via solid-state explosion of organic single crystals. The explosive reaction is realized by the Bergman reaction (cycloaromatization) of three enediyne groups on 2,3,6,7,14,15-hexaethynyl-9,10-dihydro-9,10-[1,2]benzenoanthracene...
November 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29150335/camptothecin-psammaplin-a-hybrids-as-topoisomerase-i-and-hdac-dual-action-inhibitors
#19
Raffaella Cincinelli, Loana Musso, Roberto Artali, Mario Guglielmi, Erminia Bianchino, Francesco Cardile, Fabiana Colelli, Claudio Pisano, Sabrina Dallavalle
Recent studies have demonstrated enhanced anticancer effects of combination therapy consisting of camptothecin derivatives and HDAC inhibitors. To exploit this synergy in a single active compound, we designed new dual-acting multivalent molecules simultaneously targeting topoisomerase I and HDAC. In particular, a selected compound containing a camptothecin and the psammaplin A scaffold showed a broad spectrum of antiproliferative activity, with IC50 values in the nanomolar range. Preliminary in vivo results indicated a strong antitumor activity on human mesothelioma primary cell line MM473 orthotopically xenografted in CD-1 nude mice and very high tolerability...
November 8, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29149777/investigating-the-association-of-adam33-single-nucleotide-polymorphisms-snps-with-susceptibility-to-allergic-asthma-in-azerbaijan-population-of-iran-a-case-control-study
#20
Ilghar Zeinaly, Mahnaz Sadeghi-Shabestrai, Zohreh Babaloo, Alireza Razavi, Mohammad Sajay-Asbaghi, Saeed Sadigh-Eteghad, Tohid Kazemi
Asthma, affecting a growing number of populations, is a clinical condition with complex cellular and genetic factors. Single nucleotide polymorphisms (SNPs) in gene coding for molecules, which play major roles in the immunopathogenesis of asthma have been considered recently as genetic predisposing factors this disease. Possible association between two SNPs in a disintegrin and metalloprotease 33 (ADAM33), which participates in airway remodeling, and susceptibility to asthma was studied in this study. 190 patients with asthma and 180 healthy controls were enrolled in this case-control study...
October 2017: Iranian Journal of Allergy, Asthma, and Immunology
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