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https://www.readbyqxmd.com/read/28735520/gemst-a-taylor-made-combination-that-reverts-neuroanatomical-changes-in-stroke
#1
Arturo Mangas, Javier Yajeya, Noelia González, Isabel Ruiz, Marianny Pernìa, Michel Geffard, Rafael Coveñas
In a single transient middle cerebral artery occlusion model of stroke and using immunohistochemical techniques, the effects of a new therapeutic approach named Gemst (a member of the Poly-L-Lysine innovative therapies) have been studied in the rat brain. The expression of inflammatory (CD45, CD11b), oxidative (NO-tryptophan, NO2-tyrosine) and indoleamine 2, 3-dioxygenase pathway (kynurenic acid, 3-hydroxy anthranilic acid) markers has been evaluated in early and late phases of stroke. For this purpose, we have developed eight highly specific monoclonal antibodies directed against some of these markers...
May 25, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735196/molecular-dynamics-simulation-of-sodium-dodecylsulfate-sds-bilayers
#2
Hongshu Zhang, Shiling Yuan, Jichao Sun, Jianqiang Liu, Haiping Li, Na Du, Wanguo Hou
Sodium dodecylsulfate (SDS) - a simple single tailed surfactant (STS) can form stable vesicles from its micellar solution without any additives under the mediation of solid surfaces. To further understand the mechanism of this transition on the molecular level, molecular dynamics simulations are performed to study segments of SDS bilayers (as part of vesicles) in the bulk solution systematically, at the moment that the lower leaflet of bilayers already detached from solid surfaces. The SDS membrane would rather keep their bilayers structure than return to micelles when the initial interdigitated degree (δi) between alkyl chains is more than 8...
July 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28735108/structural-biology-outside-the-box-inside-the-cell
#3
REVIEW
Jürgen M Plitzko, Benjamin Schuler, Philipp Selenko
Recent developments in cellular cryo-electron tomography, in-cell single-molecule Förster resonance energy transfer-spectroscopy, nuclear magnetic resonance-spectroscopy and electron paramagnetic resonance-spectroscopy delivered unprecedented insights into the inner workings of cells. Here, we review complementary aspects of these methods and provide an outlook toward joint applications in the future.
July 20, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28734966/development-of-new-ganglioside-probes-and-unraveling-of-raft-domain-structure-by-single-molecule-imaging
#4
REVIEW
Kenichi G N Suzuki, Hiromune Ando, Naoko Komura, Takahiro Fujiwara, Makoto Kiso, Akihiro Kusumi
Gangliosides are involved in a variety of biological roles and are a component of lipid rafts found in cell plasma membranes (PMs). Gangliosides are especially abundant in neuronal PMs and are essential to their physiological functions. However, the dynamic behaviors of gangliosides have not been investigated in living cells due to a lack of fluorescent probes that behave like their parental molecules. We have recently developed, using an entirely chemical method, four new ganglioside probes (GM1, GM2, GM3, and GD1b) that act similarly to their parental molecules in terms of raft partitioning and binding affinity...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734795/monoclonal-antibodies-in-multiple-myeloma-a-new-wave-of-the-future
#5
REVIEW
Daniel W Sherbenou, Tomer M Mark, Peter Forsberg
In 2015, 2 monoclonal antibodies were approved for the treatment of relapsed or refractory multiple myeloma (RRMM), elotuzumab and daratumumab. Elotuzumab is a monoclonal IgG-κ antibody directed against SLAMF7 (signaling lymphocytic activation molecule F7), a cell surface receptor involved in natural killer cell activation. Daratumumab is a monoclonal IgG-κ antibody that binds to CD38, a transmembrane protein found on the surface of myeloma cells and responsible for cellular adhesion and ectoenzymatic activity...
June 27, 2017: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/28734608/sequence-specific-dna-solid-phase-extraction-in-an-on-chip-monolith-towards-detection-of-antibiotic-resistance-genes
#6
Radim Knob, Daniel B Nelson, Richard A Robison, Adam T Woolley
Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface...
July 10, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28734477/polarity-sensitive-probes-for-superresolution-stimulated-emission-depletion-microscopy
#7
Erdinc Sezgin, Falk Schneider, Victoria Zilles, Iztok Urbančič, Esther Garcia, Dominic Waithe, Andrey S Klymchenko, Christian Eggeling
The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy...
July 19, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28734307/a-confinement-induced-spectroscopic-study-of-noble-gas-atoms-using-equation-of-motion-architecture-encapsulation-within-fullerene-s-voids
#8
Supriya K Chaudhuri, Rajat K Chaudhuri, Prasanta K Mukherjee, Sudip Chattopadhyay
A relativistic study of spectroscopic properties of the endohedral fullerenes Ng@C60(q) (where Ng = He, Ne and q=0,±1,±2 are the charges) associated with the C60 molecule has been done using the equation of motion coupled cluster (EOM-CC) methodology. Specific properties estimated are the transition energies, dipole oscillator strengths, and transition probabilities for the low-lying excitations 1s(2)((1)S0) → 1snp ((1)P1) (n = 2, 3, 4) for He@C60(q) and 1s(2)2s(2)2p(6) ((1)S0) → 1s(2)2s(2)2p(5)ns∕nd ((1)P1) (n = 3, 4) for Ne@C60(q), which have been compared with those for the isolated atom to depict the confinement effect of the host molecule on the encapsulated atom...
July 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28734292/single-file-mobility-of-water-like-fluid-in-a-generalized-frenkel-kontorova-model
#9
Patricia Ternes, Alejandro Mendoza-Coto, Evy Salcedo
In this work, we used a generalized Frenkel-Kontorova model to study the mobility of water molecules inside carbon nanotubes with small radius at low temperatures. Our simulations show that the mobility of confined water decreases monotonically increasing the amplitude of the substrate potential at fixed commensurations. On the other hand, the mobility of the water molecules shows a non-monotonic behavior when varying the commensuration. This result indicates that the mobility of the confined fluid presents different behavior regimes depending on the amplitude of the water-nanotube interaction...
July 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28734282/cluster-expansion-of-the-solvation-free-energy-difference-systematic-improvements-in-the-solvation-of-single-ions
#10
Josefredo R Pliego
The cluster expansion method has been used in the imperfect gas theory for several decades. This paper proposes a cluster expansion of the solvation free energy difference. This difference, which results from a change in the solute-solvent potential energy, can be written as the logarithm of a finite series. Similar to the Mayer function, the terms in the series are related to configurational integrals, which makes the integrand relevant only for configurations of the solvent molecules close to the solute. In addition, the terms involve interaction of solute with one, two, and so on solvent molecules...
July 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28733956/amyloid-beta-and-tau-as-alzheimer-s-disease-blood-biomarkers-promise-from-new-technologies
#11
REVIEW
Lih-Fen Lue, Andre Guerra, Douglas G Walker
The utility of the levels of amyloid beta (Aβ) peptide and tau in blood for diagnosis, drug development, and assessment of clinical trials for Alzheimer's disease (AD) has not been established. The lack of availability of ultra-sensitive assays is one critical issue that has impeded progress. The levels of Aβ species and tau in plasma and serum are much lower than levels in cerebrospinal fluid. Furthermore, plasma or serum contain high levels of assay-interfering factors, resulting in difficulties in the commonly used singulex or multiplex ELISA platforms...
July 2017: Neurology and Therapy
https://www.readbyqxmd.com/read/28733905/glp-1-glucagon-receptor-co-agonism-for-treatment-of-obesity
#12
REVIEW
Miguel A Sánchez-Garrido, Sara J Brandt, Christoffer Clemmensen, Timo D Müller, Richard D DiMarchi, Matthias H Tschöp
Over a relatively short period, obesity and type 2 diabetes have come to represent a large medical and economic burden to global societies. The epidemic rise in the prevalence of obesity has metabolic consequences and is paralleled by an increased occurrence of other diseases, such as diabetes, cancer and cardiovascular complications. Together, obesity and type 2 diabetes constitute one of the more preventable causes of premature death and the identification of novel, safe and effective anti-obesity drugs is of utmost importance...
July 21, 2017: Diabetologia
https://www.readbyqxmd.com/read/28733599/nanoscale-investigation-of-generation-1-pamam-dendrimers-interaction-with-a-protein-nanopore
#13
Alina Asandei, Andrei Ciuca, Aurelia Apetrei, Irina Schiopu, Loredana Mereuta, Chang Ho Seo, Yoonkyung Park, Tudor Luchian
Herein, we describe at uni-molecular level the interactions between poly(amidoamine) (PAMAM) dendrimers of generation 1 and the α-hemolysin protein nanopore, at acidic and neutral pH, and ionic strengths of 0.5 M and 1 M KCl, via single-molecule electrical recordings. The results indicate that kinetics of dendrimer-α-hemolysin reversible interactions is faster at neutral as compared to acidic pH, and we propose as a putative explanation the fine interplay among conformational and rigidity changes on the dendrimer structure, and the ionization state of the dendrimer and the α-hemolysin...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733405/analysis-of-dna-replication-in-fission-yeast-by-combing
#14
Divya R Iyer, Shankar Das, Nick Rhind
DNA replication studies based on population experiments give an average estimate of replication kinetics from many cells. This average replication profile masks the stochastic nature of origin firing in eukaryotes, which is revealed by using single-molecule techniques, such as DNA combing. The analysis of replication kinetics by DNA combing involves isolating DNA from cells that have been pulse-labeled with thymidine analogs and stretching it on a silanized coverslip. The analog-labeled patches on the stretched DNA fibers can then be detected using fluorescent antibodies against the analog...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28732159/remote-single-molecule-switching-identification-and-nano-engineering-of-hot-electron-induced-tautomerization
#15
Jens Kügel, Markus Leisegang, Markus Böhme, Andreas Krönlein, Aimee Sixta, Matthias Bode
Molecular electronics where single molecules perform basic functionalities of digital circuits is a fascinating concept that one day may augment or even replace nowadays semiconductor technologies. The tautomerization of molecules, i.e., the bistable functional position of hydrogen protons within an organic frame, has recently been intensively discussed as a potential avenue towards nano-scale switches. It has been shown that tautomerization can be triggered locally or non-locally, i.e. by a scanning tunneling microscope (STM) tip positioned directly above or in close vicinity to the molecule...
July 21, 2017: Nano Letters
https://www.readbyqxmd.com/read/28732132/entrapment-of-amino-acids-in-gas-phase-surfactant-assemblies-the-case-of-tryptophan-confined-in-positively-charged-1r-2s-dodecyl-2-hydroxy-1-methyl-2-phenylethyl-dimethylammonium-bromide-aggregates
#16
David Bongiorno, Valentina Calabrese, Leopoldo Ceraulo, Serena Indelicato, Vincenzo Turco Liveri
The ability of positively charged aggregates of the surfactant (1R,2S)-Dodecyl(2-hydroxy-1-methyl-2-phenylethyl)dimethylammonium bromide (DMEB) to incorporate D- or L-tryptophan in the gas-phase has been investigated by electrospray ion mobility mass spectrometry (ESI-IM-MS). Strongly impacted by the pH of the electrosprayed solutions, both protonated (T(+) ) and deprotonated (T(-) ) tryptophan are effectively included into the aggregates whereas, tryptophan in zwitterionic (T(0) ) form is practically absent in singly charged DMEB aggregates but can be found in multiply charged ones...
July 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28731759/single-molecule-investigation-of-energy-dynamics-in-a-coupled-plasmon-exciton-system
#17
Hiroshi Imada, Kuniyuki Miwa, Miyabi Imai-Imada, Shota Kawahara, Kensuke Kimura, Yousoo Kim
We investigate the near-field interaction between an isolated free-base phthalocyanine molecule and a plasmon localized in the gap between an NaCl-covered Ag(111) surface and the tip apex of a scanning tunneling microscope. When the tip is located in the close proximity of the molecule, asymmetric dips emerge in the broad luminescence spectrum of the plasmon generated by the tunneling current. The origin of the dips is explained by energy transfer between the plasmon and molecular excitons and a quantum mechanical interference effect, where molecular vibrations provide additional degrees of freedom in the dynamic process...
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731700/nanomanipulation-of-individual-dna-molecules-covered-by-single-layered-reduced-graphene-oxide-sheets-on-a-solid-substrate
#18
Ying Wang, Yue Shen, Bin Li, Shuo Wang, Jinjin Zhang, Yi Zhang, Jun Hu
Nanomanipulation of single DNA molecules has great potential in fundamental genetic research and clinical analysis, and is a good model system for studying the interfacial effects on physiochemical processes, which occur when manipulating the linear DNA molecules with an atomic force microscope (AFM) tip. Here, we demonstrate that AFM nanomanipulation can be carried out on DNA molecules covered by a single-layered reduced graphene oxide sheet. Nanomanipulation, which includes cutting, pushing, and sweeping operations, specific to the covered DNA molecules can be achieved in a well-controlled manner using AFM in the PeakForce Quantitative Nano-Mechanics mode...
July 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28731689/dinuclear-coordination-compounds-based-on-a-5-nitropicolinic-carboxylate-ligand-with-single-molecule-magnet-behavior
#19
Álvaro Raya-Barón, Itziar Oyarzabal, Francisco M Arrabal-Campos, José Manuel Seco, Antonio Rodríguez-Diéguez, Ignacio Fernández
Isostructural dinuclear dysprosium and yttrium coordination compounds based on the 5-nitropicolinic carboxylate ligand were synthesized and characterized. The formation of these air-stable complexes is achieved via solvothermal routes employing water as the reaction solvent. The dysprosium-based complex exhibits single-molecule magnet behavior with frequency dependence of the out-of-phase susceptibility at zero direct-current field. High-resolution mass spectrometry (electrospray ionization) experiments and advanced NMR methods including diffusion NMR techniques were applied on the diamagnetic yttrium analogue and established that these species retained their solid-state structure in solution with hydrodynamic radii of 6...
July 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28731566/heterogeneity-in-kinesin-function
#20
Babu J N Reddy, Suvranta Tripathy, Michael Vershinin, Marvin Tanenbaum, Jing Xu, Michelle Mattson-Hoss, Karim Arabi, Dail Chapman, Tory Doolin, Changbong Hyeon, Steven P Gross
The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors' velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of 'identical" motors. When moving on microtubules, slow and fast motors are persistently slow, and fast, respectively...
July 21, 2017: Traffic
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