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https://www.readbyqxmd.com/read/28535412/selective-separation-and-determination-of-glucocorticoids-in-cosmetics-using-dual-template-magnetic-molecularly-imprinted-polymers-and-hplc
#1
Min Liu, Xiaoyan Li, Junjie Li, Zongyuan Wu, Fang Wang, Li Liu, Xuecai Tan, Fuhou Lei
Molecularly imprinting polymers (MIPs) are typically prepared using a single template molecule, which allows selective separation and enrichment of only one target analyte. It is not suitable for determination of complex real samples containing multiple analytes. In order to expand the practical application of imprinted polymers, novel dual-template magnetic molecularly imprinted polymers (MMIPs) were synthesized by surface polymerization using hydrocortisone and dexamethasone as the dual-template molecules in this study...
May 17, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28534912/prediction-on-the-light-assisted-exfoliation-of-multilayered-arsenene-by-the-photo-isomerization-of-azobenzene
#2
Jun Zhao, Chunyan Liu, Wanlin Guo, Jing Ma
The exfoliation of gray arsenic into single- or few-layered arsenene is a challenge of utmost importance. We propose here that the conformational change in the photo-isomerization of azobenzene-based photochromes might be used to promote the exfoliation of multilayered arsenene. Our density functional theory calculations show that the trans-to-cis conformational change can lead to an increase of the adjacent interlayer distance of arsenene from 6.59 Å to 11.07 Å. Starting from the transition state, the azobenzene molecule can be isomerized into the more stable trans form based on further ab initio molecular dynamics simulations...
May 23, 2017: Nanoscale
https://www.readbyqxmd.com/read/28534896/coupling-between-the-photo-excited-cyclic-%C3%AF-system-and-the-4f-electronic-system-in-a-lanthanide-single-molecule-magnet
#3
K Kizaki, H Ozawa, T Kobayashi, R Matsuoka, Y Sakaguchi, A Fuyuhiro, T Fukuda, N Ishikawa
A new type of electronic interaction which couples two angular momenta, i.e. the angular momentum of a localized 4f system (J) and an orbital angular momentum generated in a cyclic π conjugated system by irradiation with a circularly-polarized light, has been identified in a lanthanide single molecule magnet.
May 23, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28534846/doses-of-immunogen-contribute-to-specificity-spectrums-of-antibodies-against-aflatoxin
#4
Peiwu Li, Jing Wu, Li Zhang, Zhiyong Fan, Tingting Yu, Feng Jiang, Xiaoqian Tang, Zhaowei Zhang, Wen Zhang, Qi Zhang
Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B₁-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M₁-BSA. The specificities of the antibodies induced, whether by aflatoxin B₁ conjugate or aflatoxin M₁ conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity...
May 19, 2017: Toxins
https://www.readbyqxmd.com/read/28534679/laser-induced-photofragmentation-fluorescence-imaging-of-alkali-compounds-in-flames
#5
Tomas Leffler, Christian Brackmann, Marcus Aldén, Zhongshan Li
Laser-induced photofragmentation fluorescence has been investigated for the imaging of alkali compounds in premixed laminar methane-air flames. An ArF excimer laser, providing pulses of wavelength 193 nm, was used to photodissociate KCl, KOH, and NaCl molecules in the post-flame region and fluorescence from the excited atomic alkali fragment was detected. Fluorescence emission spectra showed distinct lines of the alkali atoms allowing for efficient background filtering. Temperature data from Rayleigh scattering measurements together with simulations of potassium chemistry presented in literature allowed for conclusions on the relative contributions of potassium species KOH and KCl to the detected signal...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28534613/flashbody-a-next-generation-fluobody-with-fluorescence-intensity-enhanced-by-antigen-binding
#6
Devina Wongso, Jinhua Dong, Hiroshi Ueda, Tetsuya Kitaguchi
Fluorescent probes are valuable tools for visualizing the spatiotemporal dynamics of molecules in living cells. Here we developed a genetically-encoded antibody probe with antigen-dependent fluorescence intensity called "Flashbody". We first created a fusion of EGFP to the single chain variable region fragment (scFv) of antibody against seven amino acids of the bone Gla protein C-terminus (BGPC7) called BGP Fluobody, which successfully showed the intracellular localization of BGPC7-tagged protein. To generate BGP Flashbody, circularly permuted GFP was inserted in between two variable region fragments, and the linkers were optimized, resulting in fluorescence intensity increase of 300% upon binding with BGPC7 in a dose-dependent manner...
May 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28534586/desorption-of-co-from-individual-ruthenium-porphyrin-molecules-on-a-copper-surface-via-an-inelastic-tunnelling-process
#7
Takuma Omiya, Paolo Poli, Heike Arnolds, Rasmita Raval, Mats Persson, Yousoo Kim
The coordination of CO to metalloporphyrins changes their electronic and magnetic properties. Here we locally desorb CO molecules from a single ruthenium tetraphenylporphyrin carbonyl (CO-RuTPP) on Cu(110) using STM. The desorption is triggered by the injection of holes into the occupied states of the adsorbate using an unusual two-carrier process.
May 23, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28534415/sequence-and-structure-dual-dependent-interaction-between-small-molecules-and-dna-for-the-detection-of-residual-silver-ions-in-as-prepared-silver-nanomaterials
#8
Zhaoyin Wang, Jian Zhao, Zijun Li, Jianchun Bao, Zhihui Dai
Investigations on interaction between small molecules and DNA are the basis of designing advanced bioanalytical systems. We herein propose a novel interaction between heterocyclic aromatic compounds (HACs) and single-stranded DNA (ssDNA). Taking methylene blue (MB) as a typical HAC, it is found that MB can interact with cytosine (C)-rich ssDNA in an enthalpy-driven process. The interaction between MB and C-rich ssDNA is sequence and structure dual-dependent: at least three consecutive C and single-stranded structure are necessary...
May 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28533752/protocatechuic-acid-a-phenolic-from-sansevieria-roxburghiana-leaves-suppresses-diabetic-cardiomyopathy-via-stimulating-glucose-metabolism-ameliorating-oxidative-stress-and-inhibiting-inflammation
#9
Niloy Bhattacharjee, Tarun K Dua, Ritu Khanra, Swarnalata Joardar, Ashis Nandy, Achintya Saha, Vincenzo De Feo, Saikat Dewanjee
Persistent hyperglycemia, impairment of redox status and establishment of inflammatory pathophysiology integrally play important role in the pathogenesis of diabetic cardiomyopathy (DC). Present study examined the therapeutic potential of protocatechuic acid isolated from the Sansevieria roxburghiana rhizomes against DC employing rodent model of type 2 diabetes (T2D). T2D was induced by high fat diet + a low-single dose of streptozotocin (35 mg/kg, i.p.). T2D rats exhibited significantly (p < 0.01) high fasting blood glucose level...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28533514/isolation-and-genomic-characterization-of-a-dehalococcoides-strain-suggests-genomic-rearrangement-during-culture
#10
Masafumi Yohda, Kentaro Ikegami, Yuto Aita, Mizuki Kitajima, Ayane Takechi, Megumi Iwamoto, Tomomi Fukuda, Noriyoshi Tamura, Junji Shibasaki, Seiji Koike, Daisuke Komatsu, Sakari Miyagi, Minoru Nishimura, Yoshihito Uchino, Akino Shiroma, Makiko Shimoji, Hinako Tamotsu, Noriko Ashimine, Misuzu Shinzato, Shun Ohki, Kazuma Nakano, Kuniko Teruya, Kazuhito Satou, Takashi Hirano, Osami Yagi
We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with Dehalococcoides spp. that have two vinyl chloride reductive dehalogenase genes, bvcA and vcrA, and a trichloroethene reductive dehalogenase gene, tceA. The metagenome analysis of the consortium by the next generation sequencer SOLiD 3 Plus suggests that a Dehalococcoides sp...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28533394/nanomechanics-of-the-substrate-binding-domain-of-hsp70-determine-its-allosteric-atp-induced-conformational-change
#11
Soumit Sankar Mandal, Dale R Merz, Maximilian Buchsteiner, Ruxandra I Dima, Matthias Rief, Gabriel Žoldák
Owing to the cooperativity of protein structures, it is often almost impossible to identify independent subunits, flexible regions, or hinges simply by visual inspection of static snapshots. Here, we use single-molecule force experiments and simulations to apply tension across the substrate binding domain (SBD) of heat shock protein 70 (Hsp70) to pinpoint mechanical units and flexible hinges. The SBD consists of two nanomechanical units matching 3D structural parts, called the α- and β-subdomain. We identified a flexible region within the rigid β-subdomain that gives way under load, thus opening up the α/β interface...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28533372/visualization-and-characterization-of-individual-type-iii-protein-secretion-machines-in-live-bacteria
#12
Yongdeng Zhang, María Lara-Tejero, Jörg Bewersdorf, Jorge E Galán
Type III protein secretion machines have evolved to deliver bacterially encoded effector proteins into eukaryotic cells. Although electron microscopy has provided a detailed view of these machines in isolation or fixed samples, little is known about their organization in live bacteria. Here we report the visualization and characterization of the Salmonella type III secretion machine in live bacteria by 2D and 3D single-molecule switching superresolution microscopy. This approach provided access to transient components of this machine, which previously could not be analyzed...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28533173/targeting-human-telomerase-rna-component-using-antisense-oligonucleotide-induces-rapid-cell-death-and-increases-ato-induced-apoptosis-in-apl-cells
#13
Leila Asghari-Kia, Davood Bashash, Ava Safaroghli-Azar, Majid Momeny, Mohsen Hamidpour, Seyed H Ghaffari
The impressive advances carried out in designing pharmacological strategies with the aim of telomerase inhibition in cancers emerged a consensus that telomerase-targeted therapies could be exciting prospect in repertoire of future cancer strategies. The results of the present study indicated that targeting telomerase using an oligonucleotide-based molecule against human telomerase RNA template (hTR ASODN) reduced the survival rate of NB4 cells and induced a caspase-3-dependent apoptosis. Our finding was even noticeable in the synergistic experiments, where we found an enhanced reduction in the viability of the cells after short-term treatment with ATO in combination with the inhibitor...
May 19, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28532662/molecular-design-of-flotation-collectors-a-recent-progress
#14
REVIEW
Guangyi Liu, Xianglin Yang, Hong Zhong
The nature of froth flotation is to selectively hydrophobize valuable minerals by collector adsorption so that the hydrophobized mineral particles can attach air bubbles. In recent years, the increasing commercial production of refractory complex ores has been urgent to develop special collectors for enhancing flotation separation efficiency of valuable minerals from these ores. Molecular design methods offer an effective way for understanding the structure-property relationship of flotation collectors and developing new ones...
May 10, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28532626/single-nucleotide-polymorphisms-of-abcc2-modulate-renal-secretion-of-endogenous-organic-anions
#15
Kienana Muhrez, Bérenger Largeau, Patrick Emond, Montigny Frédéric, Jean-Michel Halimi, Patrick Trouillas, Chantal Barin-LE Guellec
The ATP-binding cassette family transporter MRP2 (multidrug resistance-associated protein 2), encoded by the ABCC2 gene, is involved in the renal excretion of numerous xenobiotics and it is likely that it also transports many endogenous molecules arising from not only normal essential metabolic processes but also from environmental toxins or food intake. We used a targeted gas chromatography-mass spectrometry metabolomics analysis to study whether endogenous organic anions are differentially excreted in urines of healthy volunteers according to their genotype for three functional single nucleotide polymorphisms (SNPs) in ABCC2...
May 19, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28532025/new-tetrahedral-zinc-halide-schiff-base-complexes-synthesis-crystal-structure-theoretical-3d-hirshfeld-surface-analyses-antimicrobial-and-thermal-studies
#16
L Taghizadeh, M Montazerozohori, A Masoudiasl, S Joohari, J M White
A new bidentate Schiff base ligand named as N,N'-bis((E)-3-phenylallylidene)butane-1,4-diamine (L) and three its zinc halide complexes were synthesized and characterized by FT-IR, (1)H NMR, (13)C NMR and UV-Vis spectroscopy. The crystal structure of zinc bromide and iodide complexes were characterized using single crystal X-ray diffraction. The two structures are isomorphous, crystallizing in the monoclinic crystal system with space group C2/c with closely similar unit cell dimensions. In these complexes, the Zn(II) ion is in the center of a distorted tetrahedral environment completed by two iminic nitrogen atoms of Schiff base ligand and two halide anions...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531887/expression-and-characterization-of-membrane-type-4-matrix-metalloproteinase-mt4-mmp-and-its-different-forms-in-melanoma
#17
Bettina Hieronimus, Julian Pfohl, Christian Busch, Lutz Graeve
BACKGROUND/AIMS: Membrane-type matrix metalloproteinases (MT-MMPs) are expressed on the cell surface and hydrolyze extracellular matrix components and signaling molecules by which they influence cancer cell migration and metastasis. Two of the six known MT-MMPs are anchored to the plasma membrane via a GPI anchor, one of which is MT4-MMP. Only little is known about MT4-MMP expression, synthesis, regulation and degradation. METHODS: We analyzed several human cancer cell lines as well as tissue homogenates using Western blotting and quantitative PCR for the expression of MT4-MMP...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28531818/pharmacophore-searching-a-potential-solution-for-correcting-unknown-ligands-unk-labelling-errors-in-protein-data-bank-pdb-s
#18
Musadiq Ibrahim, Adrian Jonathan Lapthorn, Mohammad Ibrahim
The Protein Data Bank (PDB) is the single most important repository of structural data for proteins and other biologically relevant molecules. Therefore, it is critically important to keep the PDB data, error-free as much as possible. In this study, we have critically examined PDB structures of 292 protein molecules which have been deposited in the repository along with potentially incorrect ligands labelled as Unknown ligands (UNK). Pharmacophores were generated for all the protein structures by using Discovery Studio Visualizer (DSV) and Accelrys, Catalyst(®)...
April 4, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28531817/electronic-properties-and-gas-adsorption-behaviour-of-pristine-silicon-and-boron-doped-8-0-single-walled-carbon-nanotube-a-first-principles-study
#19
Mohd Asyadi Azam, Farizul Muiz Alias, Liew Weng Tack, Raja Noor Amalina Raja Seman, Mohamad Fariz Mohamad Taib
Carbon nanotubes (CNTs) have received enormous attention due to their fascinating properties to be used in various applications including electronics, sensing, energy storage and conversion. The first principles calculations within density functional theory (DFT) have been carried out in order to investigate the structural, electronic and optical properties of un-doped and doped CNT nanostructures. O2, CO2, and CH3OH have been chosen as gas molecules to study the adsorption properties based on zigzag (8,0) SWCNTs...
May 5, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28531195/livestock-metabolomics-and-the-livestock-metabolome-a-systematic-review
#20
Seyed Ali Goldansaz, An Chi Guo, Tanvir Sajed, Michael A Steele, Graham S Plastow, David S Wishart
Metabolomics uses advanced analytical chemistry techniques to comprehensively measure large numbers of small molecule metabolites in cells, tissues and biofluids. The ability to rapidly detect and quantify hundreds or even thousands of metabolites within a single sample is helping scientists paint a far more complete picture of system-wide metabolism and biology. Metabolomics is also allowing researchers to focus on measuring the end-products of complex, hard-to-decipher genetic, epigenetic and environmental interactions...
2017: PloS One
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