keyword
MENU ▼
Read by QxMD icon Read
search

Stat-3

keyword
https://www.readbyqxmd.com/read/28086773/pleurotus-giganteus-berk-karun-hyde-the-giant-oyster-mushroom-inhibits-no-production-in-lps-h2o2-stimulated-raw-264-7-cells-via-stat-3-and-cox-2-pathways
#1
Asweni Baskaran, Kek Heng Chua, Vikineswary Sabaratnam, Mani Ravishankar Ram, Umah Rani Kuppusamy
BACKGROUND: Pleurotus giganteus (Berk. Karunarathna and K.D. Hyde), has been used as a culinary mushroom and is known to have medicinal properties but its potential as an anti-inflammatory agent to mitigate inflammation triggered diseases is untapped. In this study, the molecular mechanism underlying the protective effect of ethanol extract of P. giganteus (EPG) against lipopolysaccharide (LPS) and combination of LPS and hydrogen peroxide (H2O2)-induced inflammation on RAW 264.7 macrophages was investigated...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28075470/combined-effects-of-atorvastatin-and-aspirin-on-growth-and-apoptosis-in-human-prostate-cancer-cells
#2
Yan He, Huarong Huang, Chelsea Farischon, Dongli Li, Zhiyun Du, Kun Zhang, Xi Zheng, Susan Goodin
Epidemiologic studies indicate the use of either statins or aspirin have beneficial effects in prostate cancer patients. The present study was undertaken to evaluate the effects and mechanisms of atorvastatin and aspirin alone or in combination in human prostate cancer cells cultured in vitro and grown as xenograft tumors in severe combined immune-deficient (SCID) mice. The growth and apoptosis in prostate cancer cells were determined by the trypan blue exclusion and propidium iodide staining assays. Activation of the nuclear factor κB (NF-κB) was measured by luciferase reporter assay, and the levels of phospho-signal transducer and activator of transcription (Stat)3 and phospho-extracellular signal-regulated kinase (Erk)1/2 were determined by western blot analysis...
January 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28063793/interleukin-6-increases-the-expression-of-key-proteins-associated-with-steroidogenesis-in-human-nci-h295r-adrenocortical-cells
#3
Janae Strickland, Stephen McIlmoil, Brice J Williams, Dennis C Seager, James P Porter, Allan M Judd
Mechanisms of interleukin-6 (IL-6)-induced cortisol release (CR) were investigated by exposing H295R cells to IL-6 and determining mRNA/protein expression (PCR/western blots) for steroidogenic enzymes (SE), steroidogenic acute regulatory protein (StAR), steroidogenic factor-1 (SF-1)(enhances SE/StAR expression), activator protein 1 (AP-1)(regulates SE/StAR expression) and adrenal hypoplasia congenita-like protein (DAX-1)(inhibits SE/StAR expression). Promoter activity of StAR (SPA) was measured by a luciferase-coupled promoter...
January 4, 2017: Steroids
https://www.readbyqxmd.com/read/28054602/identification-of-guanosine-5-diphosphate-as-potential-iron-mobilizer-preventing-the-hepcidin-ferroportin-interaction-and-modulating-the-interleukin-6-stat-3-pathway
#4
Stanzin Angmo, Neha Tripathi, Sheenu Abbat, Shailesh Sharma, Shelley Sardul Singh, Avishek Halder, Kamalendra Yadav, Geeta Shukla, Rajat Sandhir, Vikas Rishi, Prasad V Bharatam, Hariom Yadav, Nitin Kumar Singhal
Hepcidin, a peptide hormone, is a key regulator in mammalian iron homeostasis. Increased level of hepcidin due to inflammatory conditions stimulates the ferroportin (FPN) transporter internalization, impairing the iron absorption; clinically manifested as anemia of inflammation (AI). Inhibiting hepcidin-mediated FPN degradation is proposed as an important strategy to combat AI. A systematic approach involving in silico, in vitro, ex vivo and in vivo studies is employed to identify hepcidin-binding agents. The virtual screening of 68,752 natural compounds via molecular docking resulted into identification of guanosine 5'-diphosphate (GDP) as a promising hepcidin-binding agent...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28035387/therapeutic-mechanisms-of-ibuprofen-prednisone-and-betamethasone-in-osteoarthritis
#5
Fenglong Sun, Yaohua Zhang, Qiang Li
The present study aimed to investigate the therapeutic mechanisms of nonsteroidal anti-inflammatory drugs (NSAIDs) and steroids in osteoarthritis (OA). The CHON‑002 human chondrocyte cell line was used in the study. The levels of the cytokines, interleukin (IL)‑1β, IL‑6, IL‑8 and IL‑10, released by cells treated with tumor necrosis factor‑α (TNF‑α) were determined by ELISA. Levels of collagen I, aggrecan, matrix metalloproteinase (MMP)‑1, MMP‑13, signal transducer and activator of transcription (STAT) 3, nuclear factor‑κB (NF‑κB) subunit p65 and inhibitory subunit of NF‑κB (IκB) following treatment with IL‑1β, IL‑6, IL‑8 or IL‑10 were assessed by western blot...
December 22, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28031326/myocyte-derived-hsp90-modulates-collagen-upregulation-via-biphasic-activation-of-stat-3-in-fibroblasts-during-cardiac-hypertrophy
#6
Ritwik Datta, Trisha Bansal, Santanu Rana, Kaberi Datta, Ratul Datta Chaudhuri, Mamta Chawla-Sarkar, Sagartirtha Sarkar
Signal transducer and activator of transcription-3 (STAT-3) mediated signalling in relation to upregulated collagen expression in fibroblasts is well defined during cardiac hypertrophy. Recent findings from our laboratory have identified Heat shock protein 90 (Hsp90) as a critical modulator of fibrotic signalling in cardiac fibroblasts in this disease milieu. The present study was therefore intended to analyze the role of Hsp90 in the STAT-3 mediated collagen upregulation process. Our data revealed a significant difference between in vivo and in vitro results pointing to a possible involvement of myocyte-fibroblast crosstalk in this process...
December 28, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28008946/htra2-suppresses-autoimmune-arthritis-and-regulates-activation-of-stat3
#7
Seung Hoon Lee, Young-Mee Moon, Hyeon-Beom Seo, Se-Young Kim, Eun-Kyung Kim, Junyeong Yi, Min-Kyung Nam, Jun-Ki Min, Sung-Hwan Park, Hyangshuk Rhim, Mi-La Cho
Rheumatoid arthritis (RA) is an autoimmune disease that is related to the induction of T helper (Th)17 cells, which secrete interleukin-17, and activation of the signal transducer and activator of transcription (STAT) 3. The expression of high-temperature requirement protein A (HtrA) 2, a serine protease involved in apoptosis, was decreased in RA patients nonresponsive to drug treatment of RA. The aim of this study was to determine whether overexpression of HtrA2 has a therapeutic effect on RA. Th17 differentiation, osteoclastogenesis, and lymphocyte activation are increased in motor neuron degeneration (mnd)2 mice, which lack HtrA2 activity because of a missense mutation (Ser276Cys) in the protease domain of HtrA2...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28000752/infergen-stimulated-macrophages-restrict-mycobacterium-tuberculosis-growth-by-autophagy-and-release-of-nitric-oxide
#8
Susanta Pahari, Nargis Khan, Mohammad Aqdas, Shikha Negi, Jagdeep Kaur, Javed N Agrewala
IFN alfacon-1 (Infergen) is a synthetic form of Interferon (IFN)-α2b. Infergen has immunomodulatory activity and is effective against hepatitis C virus. However, the effect of Infergen (IFG) on Mycobacterium tuberculosis (Mtb) has not yet been reported. Therefore, for the first time, we have studied the influence of IFG in constraining the survival of Mtb in human macrophages. We observed that IFG significantly enhanced the maturation and activation of macrophages. Further, it substantially augmented the secretion of IL-6, nitric oxide (NO) and antigen uptake...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27998770/microrna-340-inhibits-the-proliferation-and-invasion-of-hepatocellular-carcinoma-cells-by-targeting-jak1
#9
Jianyong Yuan, Hongxiang Ji, Feng Xiao, Zhipeng Lin, Xijun Zhao, Zhouchong Wang, Jun Zhao, Junhua Lu
Increasing evidence indicates that dysregulation of microRNAs (miRNAs) contributes to tumorigenesis. MicroRNA-340 (miR-340) is downregulated in several types of cancer. However, the functional mechanism of miR-340 in hepatocellular carcinoma (HCC) remains unclear. Here, we showed that miR-340 was significantly downregulated in HCC tissues and cell lines. Gain-of-function experiments demonstrated that miR-340 overexpression inhibited HCC cell proliferation, migration, and invasion in vitro, and suppressed tumor growth in vivo...
December 18, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27974423/vegf165b-modulates-endothelial-vegfr1-stat3-signaling-pathway-and-angiogenesis-in-human-and-experimental-peripheral-arterial-disease
#10
Vijay C Ganta, Min Choi, Anna Kutateladze, Brian H Annex
RATIONALE: Atherosclerotic-arterial occlusions decrease tissue perfusion causing ischemia to lower limbs in patients with peripheral arterial disease (PAD). Ischemia in muscle induces an angiogenic response but the magnitude of this response is frequently inadequate to meet tissue perfusion requirements. Alternate splicing in the exon-8 of vascular endothelial growth factor (VEGF)-A results in production of pro-angiogenic VEGFxxxa isoforms (VEGF165a, 165 for the 165 amino acid product) and anti-angiogenic VEGFxxxb (VEGF165b) isoforms...
December 14, 2016: Circulation Research
https://www.readbyqxmd.com/read/27895788/effects-of-microrna-21-on-the-biological-functions-of-t-cell-acute-lymphoblastic-lymphoma-leukemia
#11
Cunzhen Shi, Xudong Zhang, Xin Li, Lei Zhang, Ling Li, Zhenchang Sun, Xiaorui Fu, Jingjing Wu, Yu Chang, Wencai Li, Qingjiang Chen, Mingzhi Zhang
Previous studies have demonstrated that microRNA-21 (miR-21) is an oncogene and is significantly upregulated in tumor tissue. However, its association with T-cell acute lymphoblastic lymphoma/leukemia (T-ALL) remains poorly understood. The aim of the present study was to investigate the effects of miR-21 on T-ALL cells by constructing Jurkat cells infected with recombinant adenovirus adv-miR-21 or adv-anti-miR-21. In addition, the target gene of miR-21 was identified by reverse transcription-quantitative polymerase chain reaction and western blotting...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27881463/sestrin-3-modulation-is-essential-for-therapeutic-efficacy-of-cucurbitacin-b-in-lung-cancer-cells
#12
Naghma Khan, Farah Jajeh, Mohammad Imran Khan, Eiman Mukhtar, Sameh M Shabana, Hasan Mukhtar
Many purified compounds from dietary sources have been investigated for their anticancer activities. The main issue with most agents is their effectiveness at high doses which generally could not be delivered to humans through dietary consumption. Here, we observed that cucurbitacin B, a tetracyclic triterpenoid present in pumpkins, gourds and squashes, exhibits antiproliferative effects on human non-small cell lung cancer (NSCLC) cells at nanomolar concentrations. Treatment with cucurbitacin B (0.2-0.6 μM; 24 h) was found to result in decrease in the viability of EGFR-wild type (A549 and H1792) and EGFR-mutant lung cancer cells (H1650 and H1975) and reduction in cell-colonies but had only minimal effect on normal human bronchial epithelial cells...
November 23, 2016: Carcinogenesis
https://www.readbyqxmd.com/read/27865301/resveratrol-inhibited-non-small-cell-lung-cancer-through-inhibiting-stat-3-signaling
#13
Xin Li, Dan Wang, Qing Chun Zhao, Tao Shi, Jun Chen
BACKGROUND: Resveratrol has demonstrated many beneficial effects against cancers; however, the mechanism remains unclear. Non-small cell lung cancer accounts for 80% of lung cancers. The present study was designed to observe the effects and related mechanisms of resveratrol on non-small cell lung cancer in in vitro A549 cells. MATERIALS AND METHODS: The anticancer effects of resveratrol were analyzed on cell viability, migration and invasion, proliferation and apoptosis...
November 2016: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/27856279/the-ros-mediated-activation-of-stat-3-vegf-signaling-is-involved-in-the-27-hydroxycholesterol-induced-angiogenesis-in-human-breast-cancer-cells
#14
Dongmei Zhu, Zhaoxia Shen, Jiao Liu, Juan Chen, Yun Liu, Chunyan Hu, Zhong Li, Yuan Li
Breast cancer (BC) is the leading cause of cancer-related mortality among females worldwide, and angiogenesis plays a crucial role in BC progression. 27-Hydroxycholesterol (27HC) is an endogenous selective estrogen receptor modulator, which promotes the growth and metastasis of BC. Here, we further found that, 27HC improved the angiogenic ability of BC in a VEGF-dependent manner. For the molecular mechanisms, on one hand, as an estrogen-like factor, 27HC enhanced the expression of VEGF by the classical ERα/VEGF signaling in ER-positive BC cells; on the other hand, in both ER-positive and ER-negative BC cells, 27HC enhanced the generation of ROS, which in turn activated the STAT-3/VEGF signaling in an ER independent manner...
December 15, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27847952/evaluation-of-functionalized-mesoporous-silica-sba-15-as-a-carrier-system-for-ph3sn-ch2-3oh-against-the-a2780-ovarian-carcinoma-cell-line
#15
Christian Bensing, Marija Mojić, Santiago Gómez-Ruiz, Sandra Carralero, Biljana Dojčinović, Danijela Maksimović-Ivanić, Sanja Mijatović, Goran N Kaluđerović
SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed...
November 16, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27832152/urocortin-treatment-improves-acute-hemodynamic-instability-and-reduces-myocardial-damage-in-post-cardiac-arrest-myocardial-dysfunction
#16
Chien-Hua Huang, Chih-Hung Wang, Min-Shan Tsai, Nai-Tan Hsu, Chih-Yen Chiang, Tzung-Dau Wang, Wei-Tien Chang, Huei-Wen Chen, Wen-Jone Chen
AIMS: Hemodynamic instability occurs following cardiac arrest and is associated with high mortality during the post-cardiac period. Urocortin is a novel peptide and a member of the corticotrophin-releasing factor family. Urocortin has the potential to improve acute cardiac dysfunction, as well as to reduce the myocardial damage sustained after ischemia reperfusion injury. The effects of urocortin in post-cardiac arrest myocardial dysfunction remain unclear. METHODS AND RESULTS: We developed a preclinical cardiac arrest model and investigated the effects of urocortin...
2016: PloS One
https://www.readbyqxmd.com/read/27829437/in-vivo-inhibition-of-mir-155-significantly-alters-post-stroke-inflammatory-response
#17
Juan Carlos Pena-Philippides, Ernesto Caballero-Garrido, Tamar Lordkipanidze, Tamara Roitbak
BACKGROUND: MicroRNA miR-155 is implicated in modulation of the inflammatory processes in various pathological conditions. In our previous studies, we demonstrated that in vivo inhibition of miR-155 promotes functional recovery after mouse experimental stroke. In the present study, we explored if this beneficial effect is associated with miR-155 inhibition-induced alterations in post-stroke inflammatory response. METHODS: Intravenous injections of a specific miR-155 inhibitor were initiated at 48 h after mouse distal middle cerebral artery occlusion (dMCAO)...
November 9, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27826299/leishmania-donovani-induced-increase-in-macrophage-bcl-2-favors-parasite-survival
#18
Rajeev Kumar Pandey, Sanjana Mehrotra, Smriti Sharma, Ramachandra Subbaraya Gudde, Shyam Sundar, Chandrima Shaha
Members of the Bcl-2 family are major regulators of apoptosis in mammalian cells, and hence infection-induced perturbations in their expression could result into elimination of the parasites or creation of a niche favoring survival. In this investigation, we uncover a novel role of host Bcl-2 in sustaining Leishmania donovani infection. A rapid twofold increase in Bcl-2 expression occurred in response to parasite challenge. Downregulation of post infection Bcl-2 increase using siRNA or functional inhibition using Bcl-2 small molecule inhibitors interfered with intracellular parasite survival confirming the necessity of elevated Bcl-2 during infection...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27825337/reno-protective-effect-and-mechanism-study-of-huang-lian-jie-du-decoction-on-lupus-nephritis-mrl-lpr-mice
#19
Xiaoli Nie, Rong Deng, Lei Xiang, Pingping Jiang, Qi Xue
BACKGROUND: Huang Lian Jie Du Decoction (HLJDD), a very famous traditional Chinese medicinal prescription, has been used for heat dissipation and detoxification in China. This study was aimed to evaluate the reno-protective effects of HLJDD against lupus nephritis (LN) in vivo in MRL/lpr mice. METHODS: Animals were administered orally every day for eight consecutive weeks except the mice of normal group and model group. Organ indexes, serum interleukin-6 (IL-6), interleukin-10 (IL-10), interferon-gamma (IFN-γ) and the anti-double stranded DNA (anti-dsDNA) antibody were tested, respectively...
November 9, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27816791/porphyromonas-gingivalis-stimulated-macrophage-subsets-exhibit-differential-induction-and-responsiveness-to-interleukin-10
#20
Andrew D Foey, Neama Habil, Khalid Al-Shaghdali, StJohn Crean
OBJECTIVES: Oral mucosal macrophages (Mϕs) determine immune responses; maintaining tolerance whilst retaining the capacity to activate defences against pathogens. Mϕ responses are determined by two distinct subsets; pro-inflammatory M1- and anti-inflammatory/regulatory M2-Mϕs. Tolerance induction is driven by M2 Mϕs, whereas M1-like Mϕs predominate in inflammation, such as that exhibited in chronic Porphyromonas gingivalis (PG) periodontal infection. Mϕ responses can be suppressed to benefit either the host or the pathogen...
January 2017: Archives of Oral Biology
keyword
keyword
40633
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"