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Shaiane Silva Tomazoni, Ernesto Cesar Pinto Leal-Junior, Lúcio Frigo, Rodney Capp Pallotta, Simone Teixeira, Patricia de Almeida, Jan Magnus Bjordal, Rodrigo Álvaro Brandão Lopes-Martins
No abstract text is available yet for this article.
October 1, 2016: Journal of Biomedical Optics
Yong Yu, Jason C H Tsang, Cui Wang, Simon Clare, Juexuan Wang, Xi Chen, Cordelia Brandt, Leanne Kane, Lia S Campos, Liming Lu, Gabrielle T Belz, Andrew N J McKenzie, Sarah A Teichmann, Gordon Dougan, Pentao Liu
Innate lymphoid cells (ILCs) functionally resemble T lymphocytes in cytotoxicity and cytokine production but lack antigen-specific receptors, and are important regulators in immune response and tissue homeostasis(1, 2). ILCs are generated from common lymphoid progenitors (CLPs), which are subsequently committed to innate lymphoid lineages in the α lymphoid progenitor (αLP), early innate lymphoid progenitor (EILP), common helper innate lymphoid progenitor (CHILP) and innate lymphoid cell progenitor (ILCP) compartments(3, 4, 5, 6, 7, 8)...
September 29, 2016: Nature
Vanessa Lima Cavalcante de Oliveira, José Antonio Silva, Andrey Jorge Serra, Rodney Capp Pallotta, Evela Aparecida Pereira da Silva, Anna Cristina de Farias Marques, Regiane Dos Santos Feliciano, Rodrigo Labat Marcos, Ernesto Cesar Pinto Leal-Junior, Paulo de Tarso Camillo de Carvalho
The objective of this study was to evaluate the effects of photobiomodulation therapy (PBMT) on inflammatory indicators, i.e., inflammatory mediators (TNF-α and CINC-1), and pain characterized by hyperalgesia and B1 and B2 receptor activation at 6, 24, and 48 h after papain-induced osteoarthritis (OA) in rats. Fifty-four rats were subjected to hyperalgesia evaluations and then divided randomly into three groups-a control group and two groups OA and OA PBMT group by using laser parameters at wavelength (808 nm), output power (50 mW), energy per point (4 Joules), power density (1...
October 10, 2016: Lasers in Medical Science
Shaiane Silva Tomazoni, Ernesto Cesar Pinto Leal-Junior, Rodney Capp Pallotta, Simone Teixeira, Patricia de Almeida, Rodrigo Álvaro Brandão Lopes-Martins
Osteoarthritis (OA) triggers increased levels of inflammatory markers, including prostaglandin (PG) E2 and proinflammatory cytokines. The elevation of cytokine levels is closely associated with increased articular tissue degeneration. Thus, the use of combination therapies may presumably be able to enhance the effects on the modulation of inflammatory markers. The present study aimed to evaluate and compare the effects of photobiomodulation therapy (PBMT), physical exercise, and topical nonsteroidal anti-inflammatory drug (NSAID) use on the inflammatory process after they were applied either alone or in different combinations...
October 10, 2016: Lasers in Medical Science
Illymack Cf de Araújo, Elenice Defune, Luciana Pf Abbade, Hélio A Miot, Matheus Bertanha, Lídia R de Carvalho, Rosana R Ferreira, Winston B Yoshida
OBJECTIVES: Compare the efficacy and safety of fibrin gel to 8% papain gel for wound dressing of venous ulcers. METHOD: Patients with chronic venous ulcers were randomly assigned to one in three groups: Group 1-fibrin gel; Group 2-8% papain gel; Group 3-carbopol gel (control). Patients were seen every 15 days during 2 months, verifying reduction of the ulcer area, local infection, exudation, and epithelization. All serious or nonserious adverse events were recorded...
October 4, 2016: Phlebology
Caroline Ceccaldi, Satu Strandman, Eve Hui, Emmanuel Montagnon, Cédric Schmitt, Anis Hadj Henni, Sophie Lerouge
Hydrogels are extensively used for tissue engineering, cell therapy or controlled release of bioactive factors. Nondestructive techniques that can follow their viscoelastic properties during polymerization, remodeling, and degradation are needed, since these properties are determinant for their in vivo efficiency. In this work, we proposed the viscoelastic testing of bilayered materials (VeTBiM) as a new method for nondestructive and contact-less mechanical characterization of soft materials. The VeTBiM method measures the dynamic displacement response of a material, to a low amplitude vibration in order to characterize its viscoelastic properties...
September 30, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Phadke Girija Gajanan, Krishnamoorthy Elavarasan, Bangalore Aswathnarayan Shamasundar
Enzymatic conversion of fish frame waste of threadfin breams (Nemipterus japonicus) to protein hydrolysate could be a solution for minimizing the pollution issues related to seafood processing operations and a way for the value addition to processing by-products. Protein hydrolysates from fish frame waste (FW) of thread fin breams (N. japonicus) were prepared and evaluated for bioactive properties such as angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant and functional properties as a function of degree of hydrolysis (DH)...
September 24, 2016: Environmental Science and Pollution Research International
Zenildo Santos Silva, Ying-Ying Huang, Lucas Freitas de Freitas, Cristiane Miranda França, Sergio Brossi Botta, Patrícia Aparecida Ana, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Alessandro Deana, Cintia Raquel Lima Leal, Renato Araujo Prates, Michael R Hamblin, Sandra Kalil Bussadori
This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue - MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films...
2016: Scientific Reports
Kenjiro Kumano, Hitomi Nishinakamura, Toshiyuki Mera, Takeshi Itoh, Hiroyuki Takahashi, Toshiyoshi Fujiwara, Shohta Kodama
Although current immunosuppression protocols improve the efficacy of clinical allogenic islet transplantation, T cell-mediated allorejection remains unresolved, and major histocompatibility complexes (MHCs) play a crucial role in this process. Papain, a cysteine protease, has the unique ability to cleave the extracellular domain of the MHC class I structure. We hypothesized that pretreatment of donor islets with papain would diminish the expression of MHC class I on islets, reducing allograft immunogenicity and contributing to prolongation of islet allograft survival...
September 2, 2016: Islets
Malcolm Brinn, Katie O'Neill, Ian Musgrave, Brian Jc Freeman, Maciej Henneberg, Jaliya Kumaratilake
BACKGROUND: There is a paucity of detailed methods describing how to harvest and process motor neurons obtained from the adult rat spinal cord. NEW METHOD: Removal of intra-cardiac perfusion step. The spinal cord is extruded intact from the rat in under 60seconds post-decapitation then processed without differentiation of ventral and dorsal regions. The temperature during processing was maintained at room temperature (22°C) except during the Papain processing step where the temperature was increased to 30°C...
September 2, 2016: Journal of Neuroscience Methods
Claudius Luziga, Bui Thi To Nga, Gabriel Mbassa, Yoshimi Yamamoto
Cathepsins B and L are two prominent members of cystein proteases with broad substrate specificity and are known to be involved in the process of intra- and extra-cellular protein degradation and turnover. The propeptide region of cathepsin L is identical to Cytotoxic T-lymphocyte antigen-2α (CTLA-2α) discovered in mouse activated T-cells and mast cells. CTLA-2α exhibits selective inhibitory activities against papain and cathepsin L. We previously demonstrated the distribution pattern of the CTLA-2α protein in mouse brain by immunohistochemistry, describing that it is preferentially localized within nerve fibre bundles than neuronal cell bodies...
August 29, 2016: Acta Histochemica
Subaran Singh, Anupriya Singh, Ashis Kumar Nandi
Senescence is the final stage of plant development. Although expression of most of the genes is suppressed during senescence, a set of genes referred as senescence-associated genes (SAGs) is induced. Arabidopsis thaliana SAG12 (AtSAG12) is one such gene that has been mostly studied for its strict association with senescence. AtSAG12 encodes a papain-like cysteine protease, expressed predominantly in senescence-associated vacuoles. Rice genome contains multiple AtSAG12 homologues (OsSAGs). OsSAG12-1, the closest structural homologue of AtSAG12, is a negative regulator of developmental and stress-induced cell death...
September 2016: Journal of Biosciences
Sergey Nikitin, Daniel Palmer, Morten Meldal, Frederik Diness
Detailed studies comparing solid-supported l- or d-amino acid adhesion peptides based on the sequence KLHRIRA were performed. Stability towards proteases and levels of cellular adhesion to the otherwise inert surface of PEGA resin were compared by using fluorescently labelled peptides. A clear difference in the peptide stability towards cleavage by subtilisin, trypsin, or papain was observed. However, all of the on-bead peptides provided an optimal surface for cell adhesion and proliferation. In long-term experiments, these properties were still found to be similar on the resins modified either with l- or with d-amino acids and unaffected by the nature of their fluorescence labelling at either terminus...
October 2016: Journal of Peptide Science: An Official Publication of the European Peptide Society
Rafiou Agoro, Julie Piotet-Morin, Jennifer Palomo, Chloé Michaudel, Solenne Vigne, Isabelle Maillet, Pauline Chenuet, Noëlline Guillou, Jessica Le Bérichel, Malgorzata Kisielow, Ulf Per Flodby, Marc Le Bert, Valérie Quesniaux, Matthias Muller, Franco Di Padova, Bernhard Ryffel, Cem Gabay, Aurélie Couturier-Maillard
Allergic asthma is characterized by a strong Th2 response with inflammatory cell recruitment and structural changes in the lung. Papain is a protease allergen disrupting the airway epithelium triggering a rapid inflammation with eosinophilia mediated by innate lymphoid cell activation (ILC2) and leading to a Th2 immune response. In this study, we focused on inflammatory responses to a single exposure to papain and showed that intranasal administration of papain results in the recruitment of inflammatory cells, including neutrophils and eosinophils with a rapid production of IL-1α, IL-1β, and IL-33...
August 28, 2016: European Journal of Immunology
Tippayarat Yoonuan, Supaporn Nuamtanong, Paron Dekumyoy, Orawan Phuphisut, Poom Adisakwattana
Cathepsin L is a cysteine protease belonging to the papain family. In parasitic trematodes, cathepsin L plays essential roles in parasite survival and host-parasite interactions. In this study, cathepsin L of the lung fluke Paragonimus pseudoheterotremus (PpsCatL) was identified and its molecular biological and immunological features characterized. A sequence analysis of PpsCatL showed that the gene encodes a 325-amino-acid protein that is most similar to P. westermani cathepsin L. The in silico three-dimensional structure suggests that PpsCatL is a pro-enzyme that becomes active when the propeptide is cleaved...
August 26, 2016: Parasitology Research
Jayanand Manjhi, Maneesh Gupta, Anvesha Sinha, Beena Rawat, Durg V Rai
Context • Osteoarthritis (OA) is one of the most prevalent chronic diseases of the musculoskeleton, causing functional disability among older adults. Management of OA includes conventional pharmacological treatments consisting primarily of nonsteroidal anti-inflammatory drugs (NSAIDs), acetaminophen, physiotherapy, and surgical procedures. The medications are not ideal therapeutic agents; NSAIDs in particular can cause serious side effects. Objective • The study was conducted to investigate the effects of Balsamodendron mukul (BDM) gum resin extract on cartilage damage and microstructural changes in the subchondral bone of rats with papain-induced, osteoarthritic knee joints...
July 2016: Alternative Therapies in Health and Medicine
Yue Ma-Lauer, Javier Carbajo-Lozoya, Marco Y Hein, Marcel A Müller, Wen Deng, Jian Lei, Benjamin Meyer, Yuri Kusov, Brigitte von Brunn, Dev Raj Bairad, Sabine Hünten, Christian Drosten, Heiko Hermeking, Heinrich Leonhardt, Matthias Mann, Rolf Hilgenfeld, Albrecht von Brunn
Highly pathogenic severe acute respiratory syndrome coronavirus (SARS-CoV) has developed strategies to inhibit host immune recognition. We identify cellular E3 ubiquitin ligase ring-finger and CHY zinc-finger domain-containing 1 (RCHY1) as an interacting partner of the viral SARS-unique domain (SUD) and papain-like protease (PL(pro)), and, as a consequence, the involvement of cellular p53 as antagonist of coronaviral replication. Residues 95-144 of RCHY1 and 389-652 of SUD (SUD-NM) subdomains are crucial for interaction...
August 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
Fenfen Lei, Qiangzhong Zhao, Dongxiao Sun-Waterhouse, Mouming Zhao
An aminopeptidase was isolated from the marine Bacillus licheniformis SWJS33 (BLAP) and purified. According to the tandem mass spectrometry, the enzyme displayed 11% amino acid identity with the aminopeptidase from Bacillus (gi|496687392). BLAP exhibited maximum activity at 60°C and pH 8.0-8.5 and had a molecular mass of 100kDa. The presence of NaCl enabled 50% improvement of enzyme activity with 10-15% NaCl being the best. The observed inactivation by EDTA and bestatin and activation by Co(2+) and Ag(+) indicated that the obtained enzyme was a metalloaminopeptidase...
January 1, 2017: Food Chemistry
Jia-Jia Song, Qian Wang, Min Du, Tian-Ge Li, Bin Chen, Xue-Ying Mao
SCOPE: Recently, casein glycomacropeptide (GMP)-derived peptide was found to possess potent antioxidant and anti-inflammatory activities. In the current study, the improvement effects and underlying molecular mechanisms of GMP-derived peptide on hepatic insulin resistance were investigated. METHODS AND RESULTS: The peptide IPPKKNQDKTE was identified from GMP papain hydrolysates by LC-ESI-MS/MS. Effects of IPPKKNQDKTE on glucose metabolism and expression levels of the hepatic insulin signaling proteins in high glucose-induced insulin-resistant HepG2 cells were evaluated...
August 9, 2016: Molecular Nutrition & Food Research
W Y Mo, R S S Lau, A C K Kwok, M H Wong
The main aim of this study was to investigate the feasibility of using soybean meal added with papain to replace half of the fishmeal used in the moist pellets (49% fishmeal and 45% trash fish) developed by the Hong Kong Agriculture, Fisheries and Conservation Department (AFCD) for culturing marine fish. Gold-lined seabream (Rhabdosargus sarba), brown spotted grouper (Epinephelus bleekeri) and pompano (Trachinotus blochii) were farmed at one of the research stations (Kat-O) of AFCD, for a period of 340 days...
August 3, 2016: Environmental Pollution
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