keyword
MENU ▼
Read by QxMD icon Read
search

papain

keyword
https://www.readbyqxmd.com/read/28214581/oxadiargyl-induced-conformational-transition-of-cystatin-isolated-from-yellow-mustard-seeds-biophysical-and-biochemical-approach
#1
Azaj Ahmed, Anas Shamsi, Bilqees Bano
Phytocystatins are thiol proteinase inhibitors crucial due to their inhibitory activity in plants. These play important roles in improving crop yield, protection against insects and pathogens and modulation of apoptosis. In this chemical era, various pesticides are being used globally to increase the crop biomass. These pesticides accumulate in plant body and produce harmful effects on plants itself by interacting with essential proteins. In this present study, we have monitored the interaction of a herbicide; oxadiargyl, with phytocystatin isolated from yellow mustard seeds (YMP) by employing spectroscopic techniques viz...
February 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28212929/structural-analysis-of-a-homogeneous-polysaccharide-from-achatina-fulica
#2
Jie Liu, Feigneng Shang, Zengming Yang, Mingyi Wu, Jinhua Zhao
Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structural of the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy ((1)H, (13)C, COSY, TOCSY, NOESY, HSQC and HMBC)...
February 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28209036/-primary-culture-and-functional-identification-of-distal-pulmonary-artery-smooth-muscle-cells-in-mice
#3
M C Li, Y Q Chen, C T Zhang, Q Jiang, W J Lu, J Wang
Objective: To establish a method of isolation and primary culture of mice distal pulmonary artery smooth muscle cells (PASMCs) and identify the functional properties. Methods: PASMCs were harvested from the distal pulmonary artery (PA) tissue of mice by enzymatic digestion of collagenaseⅠand papain; and the growth characteristics were observed under inverted microscope and identified by Immunofluorescence technique. Effects on the intracellular calcium ion concentration of distal PASMCs were detected by Fura-2-AM fluorescent probe tracer under a fluorescence microscope in Krebs solution containing clopiazonic acid (CPA) and nifedipin (Nif)...
February 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28188928/the-papain-like-cysteine-proteinases-nbcysp6-and-nbcysp7-are-highly-processive-enzymes-with-substrate-specificities-complementary-to-nicotiana-benthamiana-cathepsin-b
#4
Melanie Paireder, Stefan Tholen, Andreas Porodko, Martin L Biniossek, Bettina Mayer, Marko Novinec, Oliver Schilling, Lukas Mach
The tobacco-related plant Nicotiana benthamiana is gaining interest as a versatile host for the production of monoclonal antibodies and other protein therapeutics. However, the susceptibility of plant-derived recombinant proteins to endogenous proteolytic enzymes limits their use as biopharmaceuticals. We have now identified two previously uncharacterized N. benthamiana proteases with high antibody-degrading activity, the papain-like cysteine proteinases NbCysP6 and NbCysP7. Both enzymes are capable of hydrolysing a wide range of synthetic substrates, although only NbCysP6 tolerates basic amino acids in its specificity-determining S2 subsite...
February 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28165668/plant-life-needs-cell-death-but-does-plant-cell-death-need-cys-proteases
#5
REVIEW
Daniela J Sueldo, Renier A L van der Hoorn
Caspases are key regulators of apoptosis in animals. This correlation has driven plant researchers for decades to look for caspases regulating Programmed Cell Death (PCD) in plants. These studies revealed caspase-like activities, caspase-related proteases and cysteine (Cys) proteases regulating PCD in plants, but identified no caspases and no conserved, apoptosis-like death pathway. Here, we critically review the evidence for Cys proteases implicated in PCD in plants. We discuss the role of papain-like Cys proteases, vacuolar processing enzymes and metacaspases in PCD during the development of tracheary elements, seed coat, suspensor and tapetum and during the hypersensitive response...
February 6, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28157418/whitening-efficacy-of-whitening-mouth-rinses-used-alone-or-in-conjunction-with-carbamide-peroxide-home-whitening
#6
Jbs Oliveira, R S Sarlo, E Bresciani, Tmf Caneppele
OBJECTIVES: The aim of this study was to compare the effectiveness of whitening mouth rinses on teeth previously whitened or not, exposed to food dyes. METHODS AND MATERIALS: One hundred twenty enamel-dentin specimens, 3 mm in diameter, were obtained from bovine incisors. The specimens were stained for 14 days in staining broth. After staining, the initial color reading was performed via a spectrophotometer CM-2600d (Konica Minolta). Half of specimens were submitted to whitening (10% carbamide peroxide [CP]) for 14 days...
February 3, 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28152273/effects-of-tissue-digestion-solutions-on-surface-properties-of-nitinol-stents
#7
Stacey J L Sullivan, Philip Stafford, Elon Malkin, Maureen L Dreher, Srinidhi Nagaraja
Analysis of explanted medical implants can provide a wealth of knowledge about device safety and performance. However, the quality of information may be compromised if the methods used to clean tissue from the device disturb the retrieved condition. Common solutions used to digest tissue may adversely affect the surface of the device and its severity can be material and processing dependent. In this study, two groups of stents made from the same material (Nitinol) were shape set in a salt pot (SP) or further processed by mechanical polishing (MP) and then immersed in one of three tissue digestion solutions (TDS): nitric acid (HNO3 ), sodium hydroxide (NaOH), or papain enzyme (papain)...
February 2, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28137851/a-mechanism-for-the-induction-of-type-2-immune-responses-by-a-protease-allergen-in-the-genital-tract
#8
Ji Eun Oh, Dong Sun Oh, Hi Eun Jung, Heung Kyu Lee
The genital mucosa is a barrier that is constantly exposed to a variety of pathogens, allergens, and external stimuli. Although both allergen exposure and parasite infections frequently occur in the genital area, the mechanism by which immune responses-particularly type 2 immunity-are induced has rarely been studied in the genital mucosa. Here, we demonstrate the induction of T helper type 2 (Th2) immunity in the genital mucosa in response to a model allergen, the protease papain. Intravaginal papain immunization induced type 2 immunity in a manner that was dependent on protease activity and the estrous phase of the mice...
January 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28135903/coagulation-and-rheological-behaviors-of-soy-milk-colloidal-dispersions
#9
Tomoyuki Fujii
Coagulation and rheological behaviors of soy milk are reviewed from the viewpoint of colloidal dispersion system. From the results of relative viscosity in the range of small oil body volume fractions, oil bodies containing oleosin behave as rigid spheres. The Krieger-Dougherty equation was found to describe relative viscosities well under high oil body volume fraction. These results indicate that oil bodies in soy milk behave as though suspended matter. Cross-linking between colloid particles occurs when the coagulant is added, and bulky clusters are formed...
January 30, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28135200/evaluating-the-effect-of-3-papain-gel-application-in-cutaneous-wound-healing-in-mice
#10
Flávia Figueiredo Azevedo, Letícia P Santanna, Vanessa C Bóbbo, Edson A Libert, Eliana P Araújo, Mário Abdalla Saad, Maria Helena M Lima
: While the US Food and Drug Administration has not approved the use of 3% papain gel in the United States, the authors feel this study adds to the literature regarding its use. INTRODUCTION: The aim of this study was to evaluate the effect of 3% papain gel on wounds in mice. MATERIALS AND METHODS: Thirty healthy C57BL mice (25-30 g) aged 10 weeks were randomly divided into 2 groups: mice treated with 3% papain gel and mice treated with placebo gel...
January 23, 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28130418/papain-cleavage-of-the-38-000-dalton-fragment-inhibits-the-binding-of-4-4-diisothiocyanostilbene-2-2-disulfonate-to-lys-539-on-the-60-000-dalton-fragment-in-human-band-3
#11
Takeo Yamaguchi, Hideaki Kojima, Shiori Kawaguchi, Maiko Shimada, Haruka Aso
Human band 3 is a 98-kDa transmembrane (TM) protein comprising 14 TM segments. Papain cleavages band 3 into 38- and 60-kDa fragments. Under vigorous conditions, the cleavage of the loop region between the TM 7 of gate domain and the TM 8 of core domain in the 38-kDa fragment produces 7- and 31-kDa fragments. Conformational changes of the TM 5 segment containing Lys-539 by cleavage of the 38-kDa fragment remain unclear. Pressure-induced hemolysis of erythrocytes was suppressed by binding of 4, 4'-diisothiocyanostilbene-2, 2'-disulfonate (DIDS) to Lys-539...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28119075/topical-ocular-treatment-with-monoclonal-antibody-fab-fragments-targeting-japanese-cedar-pollen-cry-j-1-inhibits-japanese-cedar-pollen-induced-allergic-conjunctivitis-in-mice
#12
Nobuaki Mizutani, Takeshi Nabe, Shin Yoshino
Fab fragments (Fabs) of antibodies having the ability only to bind to specific allergens lack effector functions due to the absence of the Fc portion. In the present study, we examined whether IgG1 monoclonal antibody (mAb) Fabs targeting Japanese cedar pollen (JCP) Cry j 1 were able to regulate JCP-induced allergic conjunctivitis in mice. BALB/c mice actively sensitized with JCP were repeatedly challenged by topical administration of JCP eye drops. Fabs prepared by the digestion of anti-JCP IgG1 mAbs (P1-3 and P1-8) with papain were applied to the eye 15min before the JCP challenges followed by measurement of the clinical conjunctivitis score...
January 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28117990/tensile-reinforcement-of-silk-films-by-the-addition-of-telechelic-type-polyalanine
#13
Kousuke Tsuchiya, Hiroyasu Masunaga, Keiji Numata
An appropriate modification technique for silk materials is needed to effectively improve their physical properties for specific applications. A telechelic-type polyalanine (T-polyA) was synthesized by papain-catalyzed polymerization as a novel reinforcing agent for silk materials. A silk fibroin obtained from Bombyx mori was homogeneously doped with T-polyA, and casting a solution of silk fibroin and T-polyA in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) resulted in a robust and transparent film. Tensile deformation studies of the silk composite film containing T-polyA with prestretching revealed that the tensile strength and toughness were enhanced relative to those of a silk-only film...
February 7, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28116769/capillary-electrophoresis-analysis-of-n-glycosylation-changes-of-serum-paraproteins-in-multiple-myeloma
#14
Zsuzsanna Kovacs, Adam Simon, Zoltan Szabo, Zsolt Nagy, Laszlo Varoczy, Ildiko Pal, Eszter Csanky, Andras Guttman
Multiple myeloma (MM) is an immedicable malignancy of the human plasma cells producing abnormal antibodies (also referred to as paraproteins) leading to kidney problems and hyperviscosity syndrome. In this paper we report on the N-glycosylation analysis of paraproteins from total human serum as well as the Fc and Fab κ/λ light chain fractions of papain digested immunoglobulins from multiple myeloma patients. Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) was used for the analysis of the N-glycans after endoglycosidase (PNGase F) mediated sugar release and fluorophore labeling (APTS)...
January 24, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28112000/evaluation-of-polyphenols-from-broussonetia-papyrifera-as-coronavirus-protease-inhibitors
#15
Ji-Young Park, Heung Joo Yuk, Hyung Won Ryu, Su Hwan Lim, Kyung Su Kim, Ki Hun Park, Young Bae Ryu, Woo Song Lee
The current study was designed to assess the inhibitory activity of Broussonetia papyrifera-derived polyphenols against 3-chymotrypsin-like and papain-like coronavirus cysteine proteases. The isolated compounds were broussochalcone B (1), broussochalcone A (2), 4-hydroxyisolonchocarpin (3), papyriflavonol A (4), 3'-(3-methylbut-2-enyl)-3',4,7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), broussoflavan A (8), kazinol F (9), and kazinol J (10). All polyphenols were more potent against papain-like protease (PL(pro)) than against 3-chymotripsin-like protease (3CL(pro)); therefore, we investigated their structural features that were responsible for this selectivity...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28099855/primary-cell-culture-of-live-neurosurgically-resected-aged-adult-human-brain-cells-and-single-cell-transcriptomics
#16
Jennifer M Spaethling, Young-Ji Na, Jaehee Lee, Alexandra V Ulyanova, Gordon H Baltuch, Thomas J Bell, Steven Brem, H Isaac Chen, Hannah Dueck, Stephen A Fisher, Marcela P Garcia, Mugdha Khaladkar, David K Kung, Timothy H Lucas, Donald M O'Rourke, Derek Stefanik, Jinhui Wang, John A Wolf, Tamas Bartfai, M Sean Grady, Jai-Yoon Sul, Junhyong Kim, James H Eberwine
Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single-cell analysis of live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We performed single-cell transcriptomics on over 300 cells, permitting identification of oligodendrocytes, microglia, neurons, endothelial cells, and astrocytes after 3 weeks in culture. Using deep sequencing, we detected over 12,000 expressed genes, including hundreds of cell-type-enriched mRNAs, lncRNAs and pri-miRNAs...
January 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/28088749/dual-channel-probe-of-carbon-dots-cooperating-with-gold-nanoclusters-employed-for-assaying-multiple-targets
#17
Kuncheng Yang, Shanshan Wang, Yingyi Wang, Hong Miao, Xiaoming Yang
Herein, carbon dots (CD@Papain) from papain have been originally synthesized in aqueous solution along with a quantum yield of 6.2%, and showed cyan fluorescence at 435nm. Meanwhile, papain-templated Au nanoclusters (AuNCs@Papain) were prepared with the same precursor. On the basis of the two types of nanomaterials, CD@Papain and AuNCs@Papain have been designed to assemble as one nanosensor (termed as CD-AuNCs@Papain) through a typical cross-linking reaction. Significantly, it not only emitted the dual-emission fluorescent signals with the same excitation, but also can assay multiple targets through exhibiting cyan, yellow, and red emission respectively...
January 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28079137/x-ray-structure-and-enzymatic-activity-profile-of-a-core-papain-like-protease-of-mers-coronavirus-with-utility-for-structure-based-drug-design
#18
Jozlyn R Clasman, Yahira M Báez-Santos, Robert C Mettelman, Amornrat O'Brien, Susan C Baker, Andrew D Mesecar
Ubiquitin-like domain 2 (Ubl2) is immediately adjacent to the N-terminus of the papain-like protease (PLpro) domain in coronavirus polyproteins, and it may play a critical role in protease regulation and stability as well as in viral infection. However, our recent cellular studies reveal that removing the Ubl2 domain from MERS PLpro has no effect on its ability to process the viral polyprotein or act as an interferon antagonist, which involves deubiquitinating and deISGylating cellular proteins. Here, we test the hypothesis that the Ubl2 domain is not required for the catalytic function of MERS PLpro in vitro...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28062237/physico-chemical-characterization-and-pharmacological-activities-of-sulfated-polysaccharide-from-sea-urchin-paracentrotus-lividus
#19
Yosra Ben Salem, Safa Amri, Khaoula Mkadmini Hammi, Amal Abdelhamid, Didier Le Cerf, Abderrahman Bouraoui, Hatem Majdoub
Sulfated polysaccharide (SP) from the eggs of sea urchin Paracentrotus lividus, extracted by papain digestion, was characterized by size exclusion chromatography coupling on-line with light scattering and viscosity detectors (SEC/MALS/VD/DRI), gas chromatography coupled to mass spectrometer (GC-MS), and Fourier transform infrared spectroscopy (FTIR) analysis. The native molecular mass of the extracted polysaccharide is high (≥22 000 KDa) and it is composed mainly of arabinose, accompanied by other monosaccharides (mostly galactose, glucose and fucose), significant amounts of uronic acids (18...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28061816/major-cys-protease-activities-are-not-essential-for-senescence-in-individually-darkened-arabidopsis-leaves
#20
Adriana Pružinská, Takayuki Shindo, Sherry Niessen, Farnusch Kaschani, Réka Tóth, A Harvey Millar, Renier A L van der Hoorn
BACKGROUND: Papain-like Cys Proteases (PLCPs) and Vacuolar Processing Enzymes (VPEs) are amongst the most highly expressed proteases during leaf senescence in Arabidopsis. Using activity-based protein profiling (ABPP), a method that enables detection of active enzymes within a complex sample using chemical probes, the activities of PLCPs and VPEs were investigated in individually darkened leaves of Arabidopsis, and their role in senescence was tested in null mutants. RESULTS: ABPP and mass spectrometry revealed an increased activity of several PLCPs, particularly RD21A and AALP...
January 6, 2017: BMC Plant Biology
keyword
keyword
40584
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"