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l-tryptophan

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https://www.readbyqxmd.com/read/28732132/entrapment-of-amino-acids-in-gas-phase-surfactant-assemblies-the-case-of-tryptophan-confined-in-positively-charged-1r-2s-dodecyl-2-hydroxy-1-methyl-2-phenylethyl-dimethylammonium-bromide-aggregates
#1
David Bongiorno, Valentina Calabrese, Leopoldo Ceraulo, Serena Indelicato, Vincenzo Turco Liveri
The ability of positively charged aggregates of the surfactant (1R,2S)-Dodecyl(2-hydroxy-1-methyl-2-phenylethyl)dimethylammonium bromide (DMEB) to incorporate D- or L-tryptophan in the gas-phase has been investigated by electrospray ion mobility mass spectrometry (ESI-IM-MS). Strongly impacted by the pH of the electrosprayed solutions, both protonated (T(+) ) and deprotonated (T(-) ) tryptophan are effectively included into the aggregates whereas, tryptophan in zwitterionic (T(0) ) form is practically absent in singly charged DMEB aggregates but can be found in multiply charged ones...
July 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28730706/chromatographic-profiles-of-tryptophan-and-kynurenine-enantiomers-derivatized-with-s-4-3-isothiocyanatopyrrolidin-1-yl-7-n-n-dimethylaminosulfonyl-2-1-3-benzoxadiazole-using-lc-ms-ms-on-a-triazole-bonded-column
#2
Hideaki Iizuka, Takahiro Harashima, Shuhei Takahashi, Ryosuke Kuwabara, Yoko Naito, Tatsuya Sakamoto, Mayu Onozato, Hideaki Ichiba, Takeshi Fukushima
d- and l-Tryptophan (Trp) and d- and l-kynurenine (KYN) were derivatized with a chiral reagent, (S)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole (DBD-PyNCS), and were separated enantiomerically by high-performance liquid chromatography (HPLC) equipped with a triazole-bonded column (Cosmosil HILIC) using tandem mass spectrometric (MS/MS) detection. Effects of column temperature, salt (HCO2 NH4 ) concentration, and pH of the mobile phase in the enantiomeric separation, followed by MS detection of (S)-DBD-PyNCS-d,l-Trp and -d,l-KYN, were investigated...
July 21, 2017: Chirality
https://www.readbyqxmd.com/read/28726163/phosphoenolpyruvate-glucose-phosphotransferase-system-modification-increases-the-conversion-rate-during-l-tryptophan-production-in-escherichia-coli
#3
Lina Liu, Sheng Chen, Jing Wu
Escherichia coli FB-04(pta1), a recombinant L-tryptophan production strain, was constructed in our laboratory. However, the conversion rate (L-tryptophan yield per glucose) of this strain is somewhat low. In this study, additional genes have been deleted in an effort to increase the conversion rate of E. coli FB-04(pta1). Initially, the pykF gene, which encodes pyruvate kinase I (PYKI), was inactivated to increase the accumulation of phosphoenolpyruvate, a key L-tryptophan precursor. The resulting strain, E...
July 19, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28715185/substrate-binding-primes-human-tryptophan-2-3-dioxygenase-for-ligand-binding
#4
Karin Nienhaus, Vincent Hahn, Manuel Hüpfel, Gerd Ulrich Nienhaus
The human heme enzyme tryptophan 2,3-dioxygenase (hTDO) catalyzes the insertion of dioxygen into its cognate substrate, L-tryptophan (L-Trp). Its active site structure is highly dynamic, and the mechanism of enzyme-substrate-ligand complex formation and the ensuing enzymatic reaction is not yet understood. Here we have studied complex formation in hTDO by using time-resolved optical and infrared spectroscopy with carbon monoxide (CO) as a ligand. We have observed that both substrate-free and substrate-bound hTDO co-exist in two discrete conformations with greatly different ligand binding rates...
July 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28702795/production-of-trans-2-3-dihydro-3-hydroxyanthranilic-acid-by-engineered-pseudomonas-chlororaphis-gp72
#5
Hongbo Hu, Yifan Li, Kaiquan Liu, Jia Zhao, Wei Wang, Xuehong Zhang
Trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) is a cyclic β-amino acid that can be used for the synthesis of chiral materials and nonnatural peptides. The aim of this study was to accumulate DHHA by engineering Pseudomonas chlororaphis GP72, a nonpathogenic strain that produces phenazine-1-carboxylic acid and 2-hydroxyphenazine. First, the phzF deletion mutant DA1 was constructed, which produced 1.91 g/L DHHA. Moreover, rpeA and pykF were disrupted and then ppsA and tktA were co-expressed in strain DA1...
July 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28678407/homochiral-metal-organic-frameworks-based-on-amino-acid-ligands-for-hplc-separation-of-enantiomers
#6
Jun-Hui Zhang, Rui-Yu Nong, Bang-Jin Wang, Ping Ai, Sheng-Ming Xie, Li-Ming Yuan
Natural amino acids are well known to form coordination polymers with transition metal ions. In this study, six homochiral metal-organic frameworks constructed from Zn(2+) or Co(2+) ions and various enantiopure amino acid (L-tyrosine, L-histidine, L-tryptophan and L-glutamic acid), namely [Zn(L-tyr)]n (L-tyrZn), [Zn4 (btc)2 (Hbtc)(L-His)2 (H2 O)4 ]·1.5H2 O, {[Zn2 (L-trp)2 (bpe)2 (H2 O)2 ]·2H2 O·2NO3 }n , [Co2 (L-Trp)(INT)2 (H2 O)2 (ClO4 )], [Co2 (sdba)((L-Trp)2 ] and [Co(L-Glu)(H2 O)·H2 O]∞ , were synthesized according to the methods previously reported in the literature...
July 5, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28669076/isolation-identification-optimization-and-metabolite-profiling-of-streptomyces-sparsus-vsm-30
#7
Ushakiranmayi Managamuri, Muvva Vijayalakshmi, V S Rama Krishna Ganduri, Satish Babu Rajulapati, Bodaiah Bonigala, B S Kalyani, Sudhakar Poda
Deep sea sediment samples of Bay of Bengal (Visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. The actinomycetes strain VSM-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. It was identified as Streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. Response surface methodology regression analysis was carried out to fit the experimental data of each response by the second-order polynomial...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28659861/the-effect-of-systemic-nitroglycerin-administration-on-the-kynurenine-pathway-in-the-rat
#8
Gábor Nagy-Grócz, Klaudia F Laborc, Gábor Veres, Attila Bajtai, Zsuzsanna Bohár, Dénes Zádori, Annamária Fejes-Szabó, Eleonóra Spekker, László Vécsei, Árpád Párdutz
The primary headache disorders include migraine, which is one of the most frequent neurological disorders, which influences more than 14% of the whole population. Despite the research efforts, its exact pathomechanism is not fully revealed, but evidence points to the role of glutamate and its receptors. Kynurenic acid is an endogenous glutamate receptor antagonist produced by the kynurenine pathway (KP). Tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) convert l-tryptophan to N-formyl-l-kynurenine, to be further transformed to l-kynurenine...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28656203/1-methyl-l-tryptophan-promotes-the-apoptosis-of-hepatic-stellate-cells-arrested-by-interferon-%C3%AE-by-increasing-the-expression-of-ifn-%C3%AE-r%C3%AE-irf-1-and-fas
#9
Ji Eun Oh, Kwang Yong Shim, Jong In Lee, Soo In Choi, Soon Koo Baik, Young Woo Eom
Liver fibrosis, a precursor to cirrhosis, is the result of the deposition of extracellular matrix (ECM) proteins and is mediated primarily by activated hepatic stellate cells (HSCs). In this study, we investigated the anti-fibrotic effects of interferon (IFN)-γ in activated HSCs in vitro and whether cell viability would be decreased by the inhibition of indoleamine 2,3-dioxygemase (IDO), which is responsible for cell cycle arrest. Following treatment with IFN-γ, cell signaling pathways and DNA content were analyzed to assess the inactivation of HSCs or the decrease in HSC proliferation...
June 26, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28650043/heme-containing-enzymes-and-inhibitors-for-tryptophan-metabolism
#10
REVIEW
Daojing Yan, Ying-Wu Lin, Xiangshi Tan
Iron-containing enzymes such as heme enzymes play crucial roles in biological systems. Three distinct heme-containing dioxygenase enzymes, tryptophan 2,3-dioxygenase (TDO), indoleamine 2,3-dioxygenase 1 (IDO1) and indoleamine 2,3-dioxygenase 2 (IDO2) catalyze the initial and rate-limiting step of l-tryptophan catabolism through the kynurenine pathway in mammals. Overexpression of these enzymes causes depletion of tryptophan and the accumulation of metabolic products, which contributes to tumor immune tolerance and immune dysregulation in a variety of disease pathologies...
June 26, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28633643/enhancement-of-thermoalkaliphilic-xylanase-production-by-pichia-pastoris-through-novel-fed-batch-strategy-in-high-cell-density-fermentation
#11
Tingting Shang, Dayong Si, Dongyan Zhang, Xuhui Liu, Longmei Zhao, Cong Hu, Yu Fu, Rijun Zhang
BACKGROUND: Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are susceptible to be inactive when suffering high temperature and high alkaline process. Therefore, it is necessary to develop a high amount of effective thermoalkaliphilic xylanases. This study aims to enhance thermoalkaliphilic xylanase production in Pichia pastoris through fermentation parameters optimization and novel efficient fed-batch strategy in high cell-density fermentation...
June 21, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28625179/dietary-l-tryptophan-leaves-a-lasting-impression-on-the-brain-and-the-stress-response
#12
Erik Höglund, Øyvind Øverli, Madelene Å Andersson, Patricia Silva, Danielle Caroline Laursen, Maria M Moltesen, Åshild Krogdahl, Joachim Schjolden, Svante Winberg, Marco A Vindas, Ian Mayer, Marie Hillestad
Comparative models suggest that effects of dietary tryptophan (Trp) on brain serotonin (5-hydroxytryptamine; 5-HT) neurochemistry and stress responsiveness are present throughout the vertebrate lineage. Moreover, hypothalamic 5-HT seems to play a central role in control of the neuroendocrine stress axis in all vertebrates. Still, recent fish studies suggest long-term effects of dietary Trp on stress responsiveness, which are independent of hypothalamic 5-HT. Here, we investigated if dietary Trp treatment may result in long-lasting effects on stress responsiveness, including changes in plasma cortisol levels and 5-HT neurochemistry in the telencephalon and hypothalamus of Atlantic salmon...
May 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28622378/gene-modification-of-the-acetate-biosynthesis-pathway-in-escherichia-coli-and-implementation-of-the-cell-recycling-technology-to-increase-l-tryptophan-production
#13
Qingyang Xu, Fang Bai, Ning Chen, Gang Bai
The implementation of a novel cell recycling technology based on a special disk centrifuge during microbial fermentation process can continuously separate the product and harmful intermediates, while maintaining the cell viability owing to the installed cooling system. Acetate accumulation is an often encountered problem in L-tryptophan fermentation by Escherichia coli. To extend our previous studies, the current study deleted the key genes underlying acetate biosynthesis to improve l-tryptophan production...
2017: PloS One
https://www.readbyqxmd.com/read/28611277/association-of-rasgrf1-methylation-with-epileptic-seizures
#14
Xiaoni Chen, Xi Peng, Liang Wang, Xinwei Fu, Ji Xiu Zhou, Binglin Zhu, Jing Luo, Xuefeng Wang, Zheng Xiao
DNA methylation, one of the mechanisms of epigenetic regulation, has been suggested to be related with epilepsy. RASgrf1 is a paternally imprinted gene and has a differentially methylated region (DMR) at the promoter that can silence gene expression. We have previously observed the down-regulation of RASgrf1 in the temporal neocortex of epilepsy patients and in the hippocampus of epileptic animals. Here, we further explored the dynamic change (1-day acute period, 10-day latent period and 45-day chronic phase) of DNA methylation and RASgrf1 expression after acute epileptic seizures in kainic acid (KA)-treated mice, and we observed the impact of N-phthalyl-L-tryptophan (RG108), a DNA methyltransferase (DNMT) inhibitor, on an acute epileptic model by polymerase chain reaction (PCR), western blotting, and bisulfite sequencing PCR (BSP)...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28599706/quantitative-chiral-analysis-of-amino-acids-in-solution-using-enantiomer-selective-photodissociation-of-cold-gas-phase-tryptophan-via-chiral-recognition
#15
Akimasa Fujihara, Naoto Maeda
To explore the origin of biomolecule homochirality in interstellar molecular clouds, enantiomer-selective photodissociation via chiral recognition between amino acids in the gas phase was examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap. Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of sodiated l-tryptophan ion, Na(+)(l-Trp), with an amino acid such as serine (Ser), threonine (Thr), or alanine (Ala) were obtained by the photo-excitation of l-Trp in the noncovalent complexes...
August 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28585544/serotonin-modulates-a-depression-like-state-in-drosophila-responsive-to-lithium-treatment
#16
Ariane-Saskia Ries, Tim Hermanns, Burkhard Poeck, Roland Strauss
Major depressive disorder (MDD) affects millions of patients; however, the pathophysiology is poorly understood. Rodent models have been developed using chronic mild stress or unavoidable punishment (learned helplessness) to induce features of depression, like general inactivity and anhedonia. Here we report a three-day vibration-stress protocol for Drosophila that reduces voluntary behavioural activity. As in many MDD patients, lithium-chloride treatment can suppress this depression-like state in flies. The behavioural changes correlate with reduced serotonin (5-HT) release at the mushroom body (MB) and can be relieved by feeding the antidepressant 5-hydroxy-L-tryptophan or sucrose, which results in elevated 5-HT levels in the brain...
June 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/28554057/the-immune-regulation-in-cancer-by-the-amino-acid-metabolizing-enzymes-arg-and-ido
#17
REVIEW
Giada Mondanelli, Stefano Ugel, Ursula Grohmann, Vincenzo Bronte
Some enzymes degrading amino acids have evolved in mammals to dampen immune responses and maintain peripheral tolerance. The enzymes metabolizing l-arginine and l-tryptophan are particularly powerful, contributing to restrain immunity towards fetal tissues and establish neonatal tolerance. Solid tumors can hijack these formidable pathways to construct a microenvironment highly unfavorable to anti-tumor T lymphocytes able to recognize them, one of mechanisms for their immune evasion. In this review, we analyze emerging concepts in the cross-talk between cells expressing these enzymes, their immune regulatory functions and pharmacological approaches that can target them to enhance cancer immunotherapy...
May 26, 2017: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/28549738/direct-actions-of-macronutrient-components-on-goldfish-hepatopancreas-in-vitro-to-modulate-the-expression-of-ghr-i-ghr-ii-igf-i-and-igf-ii-mrnas
#18
Juan Ignacio Bertucci, Ayelén Melisa Blanco, Luis Fabián Canosa, Suraj Unniappan
In mammals and fish, somatic growth and metabolism are coordinated by the GH-IGF axis, composed of growth hormone (GH), growth hormone receptors I and II (GHR-I and GHR-II), and the insulin-like growth factors I and II (IGF-I and IGF-II). In order to determine if dietary macronutrients regulate the hepatopancreatic expression of ghr-I, ghr-II, igf-I and igf-II independently of circulating GH, organ culture experiments were conducted. Briefly, goldfish hepatopancreas sections were incubated with different doses of glucose; L-tryptophan; oleic acid; linolenic acid (LNA); eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)...
May 24, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28540509/short-term-supplementation-of-isocaloric-meals-with-l-tryptophan-affects-pig-growth
#19
H N Liu, C-A A Hu, M M Bai, Gang Liu, M C B Tossou, K Xu, F N Li, P Liao, X F Kong, X Wu, Y L Yin
L-Tryptophan (Trp) and some of its metabolites regulate the circadian rhythm in mammals. We aimed to investigate the effects of short-term supplementation of Trp in isocaloric meals on growth performance using the parameters of multiple blood biomarkers and free amino acids in growing pigs. A total of 32 Landrace × Yorkshire barrows with a mean body weight of 8.64 (±1.13) kg were randomly assigned to four groups and then fed with various concentrations of Trp diets daily. Our results showed that sequential supplementation of different concentrations of Trp in isocaloric meals decreased the feed:gain (F:G) ratio (P = 0...
May 24, 2017: Amino Acids
https://www.readbyqxmd.com/read/28533791/jasmonoyl-l-tryptophan-disrupts-iaa-activity-through-the-aux1-auxin-permease
#20
Paul Staswick, Martha Rowe, Edgar P Spalding, Bessie L Splitt
Amide-linked conjugates between tryptophan (Trp) and jasmonic (JA) or indole-3-acetic (IAA) acids interfered with gravitropism and other auxin-dependent activities in Arabidopsis, but the mechanism was unclear. To identify structural features necessary for activity several additional Trp conjugates were synthesized. The phenylacetic acid (PAA) conjugate was active, while several others were not. Common features of active conjugates is that they have ring structures that are linked to Trp through an acetic acid side chain, while longer or shorter linkages are inactive or less active...
2017: Frontiers in Plant Science
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