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https://www.readbyqxmd.com/read/28344589/characterization-of-genes-encoding-key-enzymes-involved-in-anthocyanin-metabolism-of-kiwifruit-during-storage-period
#1
Boqiang Li, Yongxiu Xia, Yuying Wang, Guozheng Qin, Shiping Tian
'Hongyang' is a red fleshed kiwifruit with high anthocyanin content. In this study, we mainly investigated effects of different temperatures (25 and 0°C) on anthocyanin biosynthesis in harvested kiwifruit, and characterized the genes encoding key enzymes involved in anthocyanin metabolism, as well as evaluated the mode of the action, by which low temperature regulates anthocyanin accumulation in 'Hongyang' kiwifruit during storage period. The results showed that low temperature could effectively enhance the anthocyanin accumulation of kiwifruit in the end of storage period (90 days), which related to the increase in mRNA levels of ANS1, ANS2, DRF1, DRF2, and UGFT2...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28317715/evaluation-of-penicillium-expansum-for-growth-patulin-accumulation-nonvolatile-compounds-and-volatile-profile-in-kiwi-juices-of-different-cultivars
#2
Yuan Wang, Tingting Shan, Yahong Yuan, Zhiwei Zhang, Chunfeng Guo, Tianli Yue
Patulin (PAT) contamination and changes of nonvolatile and volatile compounds caused by Penicillium expansum in fresh juices of 8 kiwi cultivars were investigated. Growth and PAT production of P. expansum were greater at 25°C than at 4°C. P. expansum grew in kiwi juices under pH ranging from 2.75 to 3.27 and produced 45.10-268.88μg/mL of PAT at 25°C. Decreases occurred in malic acid and soluble protein; while consumption and production simultaneously happened for reducing sugar, total soluble solid, titratable acid, citric acid, ascorbic acid and total phenolics...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28298689/postharvest-application-of-brassica-meal-derived-allyl-isothiocyanate-to-kiwifruit-effect-on-fruit-quality-nutraceutical-parameters-and-physiological-response
#3
Luisa Ugolini, Laura Righetti, Katya Carbone, Roberta Paris, Lorena Malaguti, Alessandra Di Francesco, Laura Micheli, Mariano Paliotta, Marta Mari, Luca Lazzeri
The use of natural compounds to preserve fruit quality and develop high value functional products deserves attention especially in the growing industry of processing and packaging ready-to-eat fresh-cut fruit. In this work, potential mechanisms underlying the effects of postharvest biofumigation with brassica meal-derived allyl-isothiocyanate on the physiological responses and quality of 'Hayward' kiwifruits were studied. Fruits were treated with 0.15 mg L(-1) of allyl-isothiocyanate vapours for 5 h and then stored in controlled atmosphere (2% O2, 4...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28262000/horizontal-gene-transfer-gone-wild-promiscuity-in-a-kiwifruit-pathogen-leads-to-resistance-to-chemical-control
#4
Steven E Lindow
No abstract text is available yet for this article.
March 6, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28251154/effects-of-six-commercial-saccharomyces-cerevisiae-strains-on-phenolic-attributes-antioxidant-activity-and-aroma-of-kiwifruit-actinidia-deliciosa-cv-wine
#5
Xingchen Li, Yage Xing, Lin Cao, Qinglian Xu, Shaohua Li, Ranran Wang, Zijing Jiang, Zhenming Che, Hongbin Lin
"Hayward" kiwifruit (Actinidia deliciosa cv.), widely planted all around the world, were fermented with six different commercial Saccharomyces cerevisiae strains (BM4×4, RA17, RC212, WLP77, JH-2, and CR476) to reveal their influence on the phenolic profiles, antioxidant activity, and aromatic components. Significant differences in the levels of caffeic acid, protocatechuate, and soluble solid content were found among wines with the six fermented strains. Wines fermented with RC212 strain exhibited the highest total phenolic acids as well as DPPH radical scavenging ability and also had the strongest ability to produce volatile esters...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28249011/transposon-insertion-libraries-for-the-characterization-of-mutants-from-the-kiwifruit-pathogen-pseudomonas-syringae-pv-actinidiae
#6
Carl H Mesarich, Jonathan Rees-George, Paul P Gardner, Fatemeh Ashari Ghomi, Monica L Gerth, Mark T Andersen, Erik H A Rikkerink, Peter C Fineran, Matthew D Templeton
Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit canker, is one of the most devastating plant diseases of recent times. We have generated two mini-Tn5-based random insertion libraries of Psa ICMP 18884. The first, a 'phenotype of interest' (POI) library, consists of 10,368 independent mutants gridded into 96-well plates. By replica plating onto selective media, the POI library was successfully screened for auxotrophic and motility mutants. Lipopolysaccharide (LPS) biosynthesis mutants with 'Fuzzy-Spreader'-like morphologies were also identified through a visual screen...
2017: PloS One
https://www.readbyqxmd.com/read/28189049/characterization-of-metabolites-in-different-kiwifruit-varieties-by-nmr-and-fluorescence-spectroscopy
#7
Nur Ashikin Abdul Hamid, Ahmed Mediani, M Maulidiani, Faridah Abas, Yong Seo Park, Hanna Leontowicz, Maria Leontowicz, Jacek Namiesnik, S Gorinstein
It is known from our previous studies that kiwifruits, which are used in common human diet, have preventive properties of coronary artery disease. This study describes a combination of (1)H NMR spectroscopy, multivariate data analyses and fluorescence measurements in differentiating of some kiwifruit varieties, their quenching and antioxidant properties. A total of 41 metabolites were identified by comparing with literature data Chenomx database and 2D NMR. The binding properties of the extracted polyphenols against HSA showed higher reactivity of studied two cultivars in comparison with the common Hayward...
January 25, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28159291/corrigendum-to-nutrient-compositions-and-antioxidant-capacity-of-kiwifruit-actinidia-and-their-relationship-with-flesh-color-and-commercial-value-food-chem-218-2017-294-304
#8
Tingting Ma, Xiangyu Sun, Jinmei Zhao, Yilin You, Yushan Lei, Guitian Gao, Jicheng Zhan
No abstract text is available yet for this article.
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28159273/synthesis-and-characterization-of-hollow-porous-molecular-imprinted-polymers-for-the-selective-extraction-and-determination-of-caffeic-acid-in-fruit-samples
#9
Dengxin Fan, Ling Jia, Haiyan Xiang, Mijun Peng, Hui Li, Shuyun Shi
Hollow porous molecular imprinted polymers (HPMIPs) were prepared using caffeic acid (CA) as template, 4-vinylpyridine (4-VP) as functional monomer, and MCM-48 as sacrificial support. Large surface area (562.7m(2)/g) of HPMIPs resulted in high binding capacity (27.26mg/g) and fast kinetic binding (40min) in comparison with solid MIPs. Equilibrium data fitted well to Freundlich equation, and adsorption process with multi-diffusion mechanisms could be described by pseudo-second order model. Selectivity of HPMIPs was favorable...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28158721/kiwifruit-svp2-gene-prevents-premature-budbreak-during-dormancy
#10
Rongmei Wu, Tianchi Wang, Ben A W Warren, Andrew C Allan, Richard C Macknight, Erika Varkonyi-Gasic
No abstract text is available yet for this article.
February 3, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28131340/sucrose-phloem-unloading-follows-an-apoplastic-pathway-with-high-sucrose-synthase-in-actinidia-fruit
#11
Cheng Chen, Yulin Yuan, Chen Zhang, Huixia Li, Fengwang Ma, Mingjun Li
Phloem unloading plays a pivotal role in photoassimilate partitioning and the accumulation of sugars in sink organs, e.g. fruit. Here, we investigated the pathway of sucrose unloading in kiwifruit (Actinidia deliciasa cv. Qinmei) using a combination of electron microscopy, transport of the phloem-mobile symplastic tracer carboxyfluorescein and enzyme activity and gene expression assays. Our structural investigation revealed that the sieve element-companion cell complex of bundles feeding the fruit flesh was symplastically isolated from its surrounding parenchyma cells throughout fruit development, whereas numerous plasmodesmata were present between the phloem parenchyma cells...
February 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28125645/gbs-based-deconvolution-of-the-surviving-north-american-collection-of-cold-hardy-kiwifruit-actinidia-spp-germplasm
#12
Arthur T O Melo, Robert S Guthrie, Iago Hale
Plant germplasm collections can be invaluable resources to plant breeders, provided they are well-characterized. After 140 years of acquisition and curation efforts by a wide and largely non-coordinated array of private and institutional actors, the current US collection of cold-hardy kiwifruit (Actinidia spp.) is rife with misclassifications, misnomers, and mix-ups. To facilitate the systematic improvement and resource-efficient curation of these species of long-recognized horticultural potential, we used genotyping-by-sequencing (GBS) data to deconvolute this historic collection...
2017: PloS One
https://www.readbyqxmd.com/read/28112597/identification-of-loci-of-pseudomonas-syringae-pv-actinidiae-involved-in-lipolytic-activity-and-their-role-in-colonization-of-kiwifruit-leaves
#13
Hitendra Kumar Patel, Patrizia Ferrante, Meng Xianfa, Sree Gowrinadh Javvadi, Sujatha Subramoni, Marco Scortichini, Vittorio Venturi
Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa), an emerging pathogen of kiwifruit plants, has recently brought about major economic losses worldwide. Genetic studies on virulence functions of Psa have not yet been reported and there is little experimental data regarding bacterial genes involved in pathogenesis. In this study, we performed a genetic screen in order to identify transposon mutants altered in the lipolytic activity as it is known that mechanisms of regulation, production and secretion of enzymes often play crucial roles in virulence of plant pathogens...
January 23, 2017: Phytopathology
https://www.readbyqxmd.com/read/28063194/evolution-of-copper-resistance-in-the-kiwifruit-pathogen-pseudomonas-syringae-pv-actinidiae-through-acquisition-of-integrative-conjugative-elements-and-plasmids
#14
Elena Colombi, Christina Straub, Sven Künzel, Matthew D Templeton, Honour C McCann, Paul B Rainey
Horizontal gene transfer can precipitate rapid evolutionary change. In 2010 the global pandemic of kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) reached New Zealand. At the time of introduction, the single clone responsible for the outbreak was sensitive to copper, however, analysis of a sample of isolates taken in 2015 and 2016 showed that a quarter were copper resistant. Genome sequences of seven strains showed that copper resistance - comprising czc/cusABC and copABCD systems - along with resistance to arsenic and cadmium, was acquired via uptake of integrative conjugative elements (ICEs), but also plasmids...
January 7, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28003537/ileal-digesta-nondietary-substrates-from-cannulated-pigs-are-major-contributors-to-in-vitro-human-hindgut-short-chain-fatty-acid-production
#15
Carlos A Montoya, Shane M Rutherfurd, Paul J Moughan
BACKGROUND: It has been assumed that short-chain fatty acids (SCFAs) in the colon originate mainly from dietary fiber fermentation. However, SCFAs in the colon are also produced from the fermentation of nondietary material. OBJECTIVES: We aimed to predict the relative contributions of dietary and nondietary substrates in the production of SCFAs with the use of a human fecal inoculum for diets containing kiwifruit as a model fiber. METHODS: Terminal ileal digesta were collected from ileal-cannulated male pigs [n = 7; mean ± SD: 41...
December 21, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27992562/two-subclasses-of-differentially-expressed-tps1-genes-and-biochemically-active-tps1-proteins-may-contribute-to-sugar-signalling-in-kiwifruit-actinidia-chinensis
#16
Charlotte Voogd, Lara A Brian, Erika Varkonyi-Gasic
Trehalose metabolism and its intermediate trehalose-6-phosphate (T6P) are implicated in sensing and signalling sucrose availability. Four class I TREHALOSE-6-PHOSPHATE SYNTHASE (TPS1) genes were identified in kiwifruit, three of which have both the TPS and trehalose-6-phosphate phosphatase (TPP) domain, while the fourth gene gives rise to a truncated transcript. The transcript with highest sequence homology to Arabidopsis TPS1, designated TPS1.1a was the most highly abundant TPS1 transcript in all examined kiwifruit tissues...
2016: PloS One
https://www.readbyqxmd.com/read/27983700/characterization-of-starch-degradation-related-genes-in-postharvest-kiwifruit
#17
Xiong Hu, Sheng Kuang, Ai-Di Zhang, Wang-Shu Zhang, Miao-Jin Chen, Xue-Ren Yin, Kun-Song Chen
Starch is one of the most important storage carbohydrates in plants. Kiwifruit typically accumulate large amounts of starch during development. The fruit retain starch until commercial maturity, and its postharvest degradation is essential for consumer acceptance. The activity of genes related to starch degradation has, however, rarely been investigated. Based on the kiwifruit genome sequence and previously reported starch degradation-related genes, 17 novel genes were isolated and the relationship between their expression and starch degradation was examined using two sets of materials: ethylene-treated (100 µL/L, 20 °C; ETH) vs...
December 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27933987/influence-of-geographical-location-of-orchards-on-green-kiwifruit-bioactive-components
#18
Ivana Giangrieco, Simona Proietti, Stefano Moscatello, Lisa Tuppo, Alberto Battistelli, Francesco La Cara, Maurizio Tamburrini, Franco Famiani, Maria Antonietta Ciardiello
Italy is one of the world's major kiwifruit producers and exporters with orchards located in different areas from the north to the south of the peninsula. This study sought to investigate for the first time the possible influence of the geographical location of kiwifruit orchards on some fruit components, selected because of their involvement in beneficial or negative effects on human health. The fruits harvested in 16 Italian areas were analyzed, and the results obtained show that the observed variations of the relative amounts of total proteins, kiwellin, the major allergen actinidin, ascorbate, polyphenols, and superoxide dismutase (SOD)-like activity seem not to be related to the geographical location of the orchards...
December 7, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27904461/outbreak-and-spread-of-bacterial-canker-of-kiwifruit-caused-by-pseudomonas-syringae-pv-actinidiae-biovar-3-in-korea
#19
Gyoung Hee Kim, Kwang-Hyung Kim, Kyeong In Son, Eu Ddeum Choi, Young Sun Lee, Jae Sung Jung, Young Jin Koh
A bacterial pathogen, Pseudomonas syringae pv. actinidiae (Psa), is a causal agent of kiwifruit bacterial canker worldwide. Psa biovar 3 (Psa3) was first detected in 2011 at an orchard in Dodeok-myeon, Goheunggun, Jeonnam Province in Korea. In this study, we present the results of an epidemiological study regarding Psa3 occurrence on kiwifruit orchards in Korea for the period of 2013 to 2015. Since the first detection of Psa3 in 2011, there was no further case reported by 2013. However, Psa3 was rapidly spreading to 33 orchards in 2014; except for three orchards in Sacheonsi, Gyeongnam Province, most cases were reported in Jeju Island...
December 2016: Plant Pathology Journal
https://www.readbyqxmd.com/read/27904460/d-psa-k-a-model-for-estimating-the-accumulated-potential-damage-on-kiwifruit-canes-caused-by-bacterial-canker-during-the-growing-and-overwintering-seasons
#20
Ki Seok Do, Bong Nam Chung, Jae Ho Joa
We developed a model, termed D-PSA-K, to estimate the accumulated potential damage on kiwifruit canes caused by bacterial canker during the growing and overwintering seasons. The model consisted of three parts including estimation of the amount of necrotic lesion in a non-frozen environment, the rate of necrosis increase in a freezing environment during the overwintering season, and the amount of necrotic lesion on kiwifruit canes caused by bacterial canker during the overwintering and growing seasons. We evaluated the model's accuracy by comparing the observed maximum disease incidence on kiwifruit canes against the damage estimated using weather and disease data collected at Wando during 1994-1997 and at Seogwipo during 2014-2015...
December 2016: Plant Pathology Journal
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