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https://www.readbyqxmd.com/read/29331889/l-ascorbic-acid-metabolism-in-an-ascorbate-rich-kiwifruit-actinidia-eriantha-benth-cv-white-during-postharvest
#1
Zhen-Ye Jiang, Yu Zhong, Jian Zheng, Maratab Ali, Guo-Dong Liu, Xiao-Lin Zheng
Kiwifruit (Actinidia eriantha Benth.) 'White', a novel cultivar with higher L-ascorbic acid (AsA) level, is registered in China. Changes in AsA, related metabolites, enzymatic activity, and gene expression associated with AsA biosynthesis and recycling process were investigated in this paper. The results indicated that AsA biosynthesis through L-galactose pathway supplemented by D-galacturonic acid pathway and AsA recycling collectively contributed to accumulating and remaining higher AsA level in kiwifruit cv...
January 6, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29331077/optimized-paired-sgrna-cas9-cloning-and-expression-cassette-triggers-high-efficiency-multiplex-genome-editing-in-kiwifruit
#2
Zupeng Wang, Shuaibin Wang, Dawei Li, Qiong Zhang, Li Li, Caihong Zhong, Yifei Liu, Hongwen Huang
Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability is variable depending on the different combinations of the synthetic guide RNA (sgRNA) and Cas9 protein expression devices. Optimizing conditions for its use within a particular species is therefore needed to achieve highly efficient genome editing...
January 13, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29242227/complete-genome-sequence-of-the-kiwifruit-pathogen-pseudomonas-syringae-pv-actinidiae-biovar-5-originating-from-japan
#3
Russell Poulter, George Taiaroa, Nicholas Sumpter, Peter Stockwell, Margi Butler
We present the first complete genome sequence of a copper-resistant biovar 5 strain of a bacterial pathogen of kiwifruit, Pseudomonas syringae pv. actinidiae. Comparison with the genome sequence of a copper-sensitive biovar 5 isolate indicates that copper resistance is encoded on a plasmid.
December 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29240520/draft-whole-genome-sequence-analyses-on-pseudomonas-syringae-pv-actinidiae-hr-negative-strains-detected-from-kiwifruit-bleeding-sap-samples
#4
Enrico Biondi, Alan Zamorano, Ernesto Vega, Stefano Ardizzi, Davide Sitta, Roberto De Salvador, Reinaldo Campos-Vargas, Claudio Meneses, Set Pérez, Assunta Bertaccini, Nicola Fiore
Kiwifruit bleeding sap samples, collected in Italian and Chilean orchards from symptomatic and asymptomatic plants, were evaluated for the presence of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker. The saps were sampled during the spring of both hemispheres, before the bud sprouting, during the optimal time window for the collection of an adequate volume of sample for the early detection of the pathogen, preliminarily by molecular assays, and then, through its direct isolation and identification...
December 14, 2017: Phytopathology
https://www.readbyqxmd.com/read/29238278/role-of-metcalfa-pruinosa-as-a-vector-for-pseudomonas-syringae-pv-actinidiae
#5
Irene Donati, Sofia Mauri, Giampaolo Buriani, Antonio Cellini, Francesco Spinelli
After 20 years of steady increase, kiwifruit industry faced a severe arrest due to the pandemic spread of the bacterial canker, caused by Pseudomonas syringae pv. actinidiae (Psa). The bacterium penetrates the host plant primarily via natural openings or wounds, and its spread is mainly mediated by atmospheric events and cultural activities. Since the role of sucking insects as vectors of bacterial pathogens is widely documented, we investigated the ability of Metcalfa pruinosa Say (1830), one of the most common kiwifruit pests, to transmit Psa to healthy plants in laboratory conditions...
December 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/29235670/presumptive-migrating-gall-bladder-mucocoele-in-two-dogs-with-gall-bladder-rupture
#6
R K Burchell, L Thornton, C K Lim, M Murakami, Y Nakamura, A Gal
A 10-year-old neutered female soft-coated wheaten terrier and a 10-year-old, entire female Pomeranian were presented for vomiting and anorexia. Using ultrasound, an oval structure with a stellate, kiwifruit-like appearance typical of a gall bladder mucocoele was observed in the caudal abdomen of the soft-coated wheaten terrier and adjacent to the liver in the Pomeranian. There was also a moderate volume of abdominal effusion in both dogs. Cytology of the peritoneal fluid indicated a sterile exudative process but varied between the two dogs, with an absence of bile pigment in the soft-coated wheaten terrier and marked bile peritonitis in the Pomeranian...
December 13, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29222611/kiwifruit-svp2-controls-developmental-and-drought-stress-pathways
#7
Rongmei Wu, Tianchi Wang, Ben A W Warren, Susan J Thomson, Andrew C Allan, Richard C Macknight, Erika Varkonyi-Gasic
Genome-wide targets of Actinidia chinensis SVP2 confirm roles in ABA- and dehydration-mediated growth repression and reveal a conservation in mechanism of action between SVP genes of taxonomically distant Arabidopsis and a woody perennial kiwifruit. The molecular mechanisms underlying growth and dormancy in woody perennials are largely unknown. In Arabidopsis, the MADS-box transcription factor SHORT VEGETATIVE PHASE (SVP) plays a key role in the progression from vegetative to floral development, and in woody perennials SVP-like genes are also proposed to be involved in controlling dormancy...
December 8, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29203778/kiwifruit-r2r3-myb-transcription-factors-and-contribution-of-the-novel-acmyb75-to-red-kiwifruit-anthocyanin-biosynthesis
#8
Wenbin Li, Zehong Ding, Mengbin Ruan, Xiaoling Yu, Ming Peng, Yifei Liu
Red kiwifruit (Actinidia chinensis) is a popular fresh fruit with a high market value due to its unique color, caused by anthocyanin accumulation. The R2R3-MYB transcription factors (TFs) have important roles in plant development and anthocyanin metabolism. In this first comprehensive study of R2R3-MYBs in kiwifruit, a total of 93 R2R3-MYB genes, including five novel previously unannotated AcMYBs, were identified. Their phylogenic relationship, exon-intron structures, and conserved motifs were analyzed. Based on transcriptome data, 60 AcMYBs were expressed (FPKM > 1) across seven developmental stages of kiwifruit, revealing five expression patterns...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29195998/transcriptome-analysis-reveals-the-effect-of-pre-harvest-cppu-treatment-on-the-volatile-compounds-emitted-by-kiwifruit-stored-at-room-temperature
#9
Jing Luo, Linlin Guo, Yunan Huang, Chao Wang, Chengkui Qiao, Rongli Pang, Jun Li, Tao Pang, Ruiping Wang, Hanzhong Xie, Jinbao Fang
Kiwifruits are rich in nutrients beneficial to humans. Because forchlorfenuron (CPPU)-treatment after full bloom can enlarge fruit size, and significantly increase the income of farmers, it has been extensively used. However, CPPU might also influence fruit sugar and acid content, and storage performance. This study analyzed the differences in volatile emissions between CPPU-treated and water-treated kiwifruits after two, four, six, or eight days of storage, and differential gene expression related to these compounds using high-throughput sequencing...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29191746/proteomics-analysis-to-understand-the-aba-stimulation-of-wound-suberization-in-kiwifruit
#10
Xueyuan Han, Wenjing Lu, Xiaopeng Wei, Li Li, Linchun Mao, Yuying Zhao
Quick suberin-based healing after wounding played a protective role for plant to prevent further damage. In this study, the stimulative effect of exogenous abscisic acid (ABA) on wound suberization in postharvest kiwifruit was evaluated through suberin staining with toluidine blue O as well as the determination of suberin phenolics and aliphatics in wound tissue. Furthermore, to reveal the regulatory involvement of ABA in wound suberization, comparative quantitative proteomics and transcriptomics analyses based on iTRAQ and qRT-PCR technique were performed...
November 27, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29181018/corrigendum-phytohormone-and-putative-defense-gene-expression-differentiates-the-response-of-hayward-kiwifruit-to-psa-and-pfm-infections
#11
Kirstin V Wurms, Allan J Hardaker, Annette Ah Chee, Judith Bowen, Janet Phipps, Joseph Taylor, Dwayne Jensen, Janine Cooney, Mark Wohlers, Tony Reglinski
[This corrects the article on p. 1366 in vol. 8, PMID: 28824694.].
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29163590/multiple-copies-of-a-simple-myb-binding-site-confers-trans-regulation-by-specific-flavonoid-related-r2r3-mybs-in-diverse-species
#12
Cyril Brendolise, Richard V Espley, Kui Lin-Wang, William Laing, Yongyan Peng, Tony McGhie, Supinya Dejnoprat, Sumathi Tomes, Roger P Hellens, Andrew C Allan
In apple, the MYB transcription factor MYB10 controls the accumulation of anthocyanins. MYB10 is able to auto-activate its expression by binding its own promoter at a specific motif, the R1 motif. In some apple accessions a natural mutation, termed R6, has more copies of this motif within the MYB10 promoter resulting in stronger auto-activation and elevated anthocyanins. Here we show that other anthocyanin-related MYBs selected from apple, pear, strawberry, petunia, kiwifruit and Arabidopsis are able to activate promoters containing the R6 motif...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29152256/consumption-of-kiwifruit-capsules-increases-faecalibacterium-prausnitzii-abundance-in-functionally-constipated-individuals-a-randomised-controlled-human-trial
#13
Paul Blatchford, Halina Stoklosinski, Sarah Eady, Alison Wallace, Christine Butts, Richard Gearry, Glenn Gibson, Juliet Ansell
This study investigated the impact of ACTAZIN™ green (2400 and 600 mg) and Livaux™ (2400 mg) gold kiwifruit supplements on faecal microbial composition and metabolites in healthy and functionally constipated (FC) participants. The participants were recruited into the healthy group (n 20; one of whom did not complete the study) and the FC group (n 9), each of whom consumed all the treatments and a placebo (isomalt) for 4 weeks in a randomised cross-over design interspersed with 2-week washout periods. Modification of faecal microbiota composition and metabolism was determined by 16S rRNA gene sequencing and GC, and colonic pH was calculated using SmartPill® wireless motility capsules...
2017: Journal of Nutritional Science
https://www.readbyqxmd.com/read/29148054/transcriptome-analysis-unravels-an-ethylene-response-factor-involved-in-regulating-fruit-ripening-in-pear
#14
Ping-Ping Hao, Guo-Ming Wang, Hai-Yan Cheng, Ya-Qi Ke, Kai-Jie Qi, Chao Gu, Shao-Ling Zhang
Ethylene response factor (ERF) has been widely studied in regulating fruit ripening in tomato, apple, banana and kiwifruit, but little is known in pear. In this study 1-methylcyclopropene (1-MCP) treatment, an inhibitor of ethylene perception, was conducted at approximately 30 days before harvest to delay fruit ripening in a climacteric white pear cultivar Yali. Transcriptome libraries were constructed and sequenced in pre-ripening, ripening, and 1-MCP treated fruits. Data analysis showed that 73 candidate genes related to fruit ripening were induced by 1-MCP, among which two were positively related, namely 1-aminocyclopropane-1-carboxyla oxidase and an ERF gene (designated as ACO54 and ERF24)...
November 16, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29144740/identification-synthesis-and-safety-assessment-of-thidiazuron-1-phenyl-3-1-2-3-thidiazol-5-yl-urea-and-its-metabolites-in-kiwifruits
#15
Zhiwei Zhang, Haihua Yang, Zhenhong Gao, Yahong Yuan, Jing Dong, Yuan Wang, Tianli Yue
Quality of kiwifruit became worse due to the abuse of plant growth regulators (PGRs). The safety of the fruits treated with PGRs are also worried by consumers. Therefore, the present study analysed the structure of thidiazuron (TDZ, (1-phenyl-3-(1,2,3-thidiazol-5-yl)urea)) (1) and its metabolites of bio-transformation in kiwifruits by using liquid chromatography hybrid ion trap time-of-flight mass spectrometry (LC-IT-TOF-MS). Standard compounds were also synthesized and used for structural identification of those metabolites...
November 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29120390/functional-characterization-of-waterlogging-and-heat-stresses-tolerance-gene-pyruvate-decarboxylase-2-from-actinidia-deliciosa
#16
Hui-Ting Luo, Ji-Yu Zhang, Gang Wang, Zhan-Hui Jia, Sheng-Nan Huang, Tao Wang, Zhong-Ren Guo
A previous report showed that both Pyruvatedecarboxylase (PDC) genes were significantly upregulated in kiwifruit after waterlogging treatment using Illumina sequencing technology, and that the kiwifruit AdPDC1 gene was required during waterlogging, but might not be required during other environmental stresses. Here, the function of another PDC gene, named AdPDC2, was analyzed. The expression of the AdPDC2 gene was determined using qRT-PCR, and the results showed that the expression levels of AdPDC2 in the reproductive organs were much higher than those in the nutritive organs...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29111643/kiwifruit-like-persistent-luminescent-nanoparticles-with-high-performance-and-in-situ-activable-near-infrared-persistent-luminescence-for-long-term-in-vivo-bioimaging
#17
Xia-Hui Lin, Liang Song, Shan Chen, Xiao-Feng Chen, Jing-Jing Wei, Jingying Li, Guoming Huang, Huang-Hao Yang
Persistent luminescence nanoparticles (PLNPs) have great potential for bioimaging because they can eliminate the tissue autofluorescence and improve the signal-to-noise ratio significantly. High-temperature calcination is a necessary process for the PLNPs to achieve high luminescence intensity and long afterglow time. However, high-temperature calcination usually results in uncontrollable morphology and poor homogeneity of PLNPs, which greatly limit their applications. Therefore, there is still a high demand to find a suitable method for synthesizing PLNPs with high luminescence intensity and long afterglow time while maintaining their monodispersed morphology...
November 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29084137/kiwifruit-non-sugar-components-reduce-glycaemic-response-to-co-ingested-cereal-in-humans
#18
Suman Mishra, Haley Edwards, Duncan Hedderley, John Podd, John Monro
Kiwifruit (KF) effects on the human glycaemic response to co-ingested wheat cereal were determined. Participants (n = 20) consumed four meals in random order, all being made to 40 g of the same available carbohydrate, by adding kiwifruit sugars (KF sug; glucose, fructose, sucrose 2:2:1) to meals not containing KF. The meals were flaked wheat biscuit (WB)+KFsug, WB+KF, WB+guar gum+KFsug, WB+guar gum+KF, that was ingested after fasting overnight. Blood glucose was monitored 3 h and hunger measured at 180 min post-meal using a visual analogue scale...
October 30, 2017: Nutrients
https://www.readbyqxmd.com/read/29057484/biochemical-and-functional-characterization-of-acufgt3a-a-galactosyltransferase-involved-in-anthocyanin-biosynthesis-in-the-red-fleshed-kiwifruit-actinidia-chinensis
#19
Yanfei Liu, Bin Zhou, Yingwei Qi, Cuihua Liu, Zhande Liu, Xiaolin Ren
Much of the diversity of anthocyanin pigmentation in plant tissues is due to the action of glycosyltransferases, which attach sugar moieties to the anthocyanin aglycone. This step can increase both their solubility and stability. We investigated the pigmentation of the outer and inner pericarps of developing fruit of the red-fleshed kiwifruit Actinidia chinensis cv. 'Hongyang'. The results show that the red color of the inner pericarp is due to anthocyanin. Based on expression analyses of structural genes, AcUFGT was shown to be the key gene involved in the anthocyanin biosynthetic pathway...
October 23, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29039307/monoglobus-pectinilyticus-gen-nov-sp-nov-a-pectinolytic-bacterium-isolated-from-human-faeces
#20
Caroline C Kim, William J Kelly, Mark L Patchett, Gerald W Tannock, Z Jordens, Halina M Stoklosinski, Jordan W Taylor, Ian M Sims, Tracey J Bell, Douglas I Rosendale
A novel anaerobic pectinolytic bacterium (strain 14(T)) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14(T) belonged to the family Ruminococcaceae, but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14(T) was Acetivibrio cellulolyticus (89.7 % sequence similarity). Strain 14(T) shared ~99 % sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
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