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https://www.readbyqxmd.com/read/29140977/low-cost-and-open-source-multi-fluorescence-imaging-system-for-teaching-and-research-in-biology-and-bioengineering
#1
Isaac Nuñez, Tamara Matute, Roberto Herrera, Juan Keymer, Timothy Marzullo, Timothy Rudge, Fernán Federici
The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates...
2017: PloS One
https://www.readbyqxmd.com/read/29140294/a-human-body-pressure-distribution-imaging-system-based-on-wavelet-analysis-and-resistance-tomography
#2
Shuanfeng Zhao, Wenbo Wang, Wei Guo, Chuanwei Zhang
In this paper, a pressure distribution sensing system based on wavelet analysis and resistance tomography is proposed to overcome the shortcomings of a traditional electrode type pressure distribution sensor, which needs to be arranged with many electrodes and has a high production cost. The system uses ADS1256, a constant current source module, a serial communication module, a Raspberry host, a touch screen, and other components. The wavelet transform is used to preprocess the collected signal to improve the anti-jamming performance of the system...
November 15, 2017: Sensors
https://www.readbyqxmd.com/read/29126004/photovoltaic-characteristics-of-natural-light-harvesting-dye-sensitized-solar-cells
#3
H S Hafez, S S Shenouda, M Fadel
In this work of research, anthocyanin as a natural dye obtained from raspberry fruits, was used and tested as a photon harvesting/electron donating dye in titanium dioxide nanoparticle-based DSSCs. A working photoelectrode made from TiO2 nanoparticles with an average particle size (10-40nm) that is coated on Florine doped tin-oxide substrate, was prepared via a simple and low cost hydrothermal method. A detailed structural and morphological analysis of the TiO2 photoactive electrode was investigated by X-ray diffraction (XRD), diffuse reflectance spectrometer, transmission electron microscope (TEM) and scanning electron microscope (SEM)...
November 7, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29118819/a-method-for-automatic-segmentation-and-splitting-of-hyperspectral-images-of-raspberry-plants-collected-in-field-conditions
#4
Dominic Williams, Avril Britten, Susan McCallum, Hamlyn Jones, Matt Aitkenhead, Alison Karley, Ken Loades, Ankush Prashar, Julie Graham
Hyperspectral imaging is a technology that can be used to monitor plant responses to stress. Hyperspectral images have a full spectrum for each pixel in the image, 400-2500 nm in this case, giving detailed information about the spectral reflectance of the plant. Although this technology has been used in laboratory-based controlled lighting conditions for early detection of plant disease, the transfer of such technology to imaging plants in field conditions presents a number of challenges. These include problems caused by varying light levels and difficulties of separating the target plant from its background...
2017: Plant Methods
https://www.readbyqxmd.com/read/29114390/phospholipase-d-inhibition-by-hexanal-is-associated-with-calcium-signal-transduction-events-in-raspberry
#5
Walid El Kayal, Gopinadhan Paliyath, J Alan Sullivan, Jayasankar Subramanian
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient (HC) was applied as a preharvest spray during fruit development. The effects of HC were assessed using physiological, biochemical and anatomical parameters on the treated fruits and compared with the effects of mock inoculation which lacked hexanal. Sugars and acidity did not show a significant change in response to HC treatment, while the pulling force (the tension required to detach the berry from the receptacle) significantly improved in the HC-treated fruits, compared to control...
2017: Horticulture Research
https://www.readbyqxmd.com/read/29111889/the-combined-effects-of-exercise-diet-and-a-multi-ingredient-dietary-supplement-on-body-composition-and-adipokine-changes-in-overweight-adults
#6
Shawn M Arent, Alan J Walker, Joseph K Pellegrino, David J Sanders, Bridget A McFadden, Tim N Ziegenfuss, Hector L Lopez
BACKGROUND: Very few weight and fat loss supplements undergo finished-product research to examine efficacy. The purpose of this study was to determine the effects of an 8-week diet and exercise program on body composition, hip and waist girth, and adipokines and evaluate whether a dietary supplement containing raspberry ketone, capsaicin, caffeine, garlic, and Citrus aurantium enhanced outcomes. METHODS: Overweight men and women completed this randomized, placebo-controlled, double-blind study...
November 7, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29109723/inhibition-of-pro-inflammatory-and-anti-apoptotic-biomarkers-during-experimental-oral-cancer-chemoprevention-by-dietary-black-raspberries
#7
Steve Oghumu, Bruce C Casto, Jennifer Ahn-Jarvis, Logan C Weghorst, Jim Maloney, Paul Geuy, Kyle Z Horvath, Claire E Bollinger, Blake M Warner, Kurt F Summersgill, Christopher M Weghorst, Thomas J Knobloch
Oral cancer continues to be a significant public health problem worldwide. Recently conducted clinical trials demonstrate the ability of black raspberries (BRBs) to modulate biomarkers of molecular efficacy that supports a chemopreventive strategy against oral cancer. However, it is essential that a preclinical animal model of black raspberry (BRB) chemoprevention which recapitulates human oral carcinogenesis be developed, so that we can validate biomarkers and evaluate potential mechanisms of action. We therefore established the ability of BRBs to inhibit oral lesion formation in a carcinogen-induced rat oral cancer model and examined potential mechanisms...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29101901/fabrication-of-shape-tunable-macroparticles-by-seeded-polymerization-of-styrene-using-non-cross-linked-starch-based-seed
#8
Xiaopeng Pei, Kankan Zhai, Xuechen Liang, Yukun Deng, Kun Xu, Ying Tan, Xianping Yao, Pixin Wang
Nonspherical colloidal particles with various geometries and different compositions have attracted tremendous attention and been widely researched. The preparation of polymer colloidal particles with controlled shapes by seeded polymerization is recognized as the most promising technique owing to the precise control of various morphologies and using non-cross-linked seed particles are of particular interest. Seeds particles derived from natural biopolymers are seldom applied. Hence, non-cross-linked starch-based seed could be used to fabricate the anisotropic particles by soap-free seed polymerization...
October 26, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29099745/a-middleware-solution-for-wireless-iot-applications-in-sparse-smart-cities
#9
Paolo Bellavista, Carlo Giannelli, Stefano Lanzone, Giulio Riberto, Cesare Stefanelli, Mauro Tortonesi
The spread of off-the-shelf mobile devices equipped with multiple wireless interfaces together with sophisticated sensors is paving the way to novel wireless Internet of Things (IoT) environments, characterized by multi-hop infrastructure-less wireless networks where devices carried by users act as sensors/actuators as well as network nodes. In particular, the paper presents Real Ad-hoc Multi-hop Peer-to peer-Wireless IoT Application (RAMP-WIA), a novel solution that facilitates the development, deployment, and management of applications in sparse Smart City environments, characterized by users willing to collaborate by allowing new applications to be deployed on their smartphones to remotely monitor and control fixed/mobile devices...
November 3, 2017: Sensors
https://www.readbyqxmd.com/read/29091813/dietary-red-raspberries-attenuate-dextran-sulfate-sodium-induced-acute-colitis
#10
Shima Bibi, Yifei Kang, Min Du, Mei-Jun Zhu
Persistent intestinal inflammation severely impairs intestinal integrity resulting in inflammatory bowel disease. Red raspberries (RB) are a rich source of bioactive compounds; their beneficial effect on the colitis protection was evaluated in the current study using a dextran sulfate sodium (DSS)-induced acute colitis mouse model. Six-week-old mice were fed a standard rodent research diet supplemented with RB (0 or 5% w/w, n=20 each group) for 6 weeks. At the 4th week of dietary treatment, approximately half of mice in each dietary group (n=12 each group) were subjected to 2...
September 14, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29090743/a-combination-of-raspberry-pi-and-softether-vpn-for-controlling-research-devices-via-the-internet
#11
Toshikazu Kuroda
Remote control over devices for experiments may increase the efficiency of operant research and expand the area where behavior can be studied. This article introduces a combination of Raspberry Pi® (Pi) and SoftEther VPN® that allows for such remote control via the Internet. The Pi is a small Linux computer with a great degree of flexibility for customization. Test results indicate that a Pi-based interface meets the requirement for conducting operant research. SoftEther VPN® allows for establishing an extensive private network on the Internet using a single private Wi-Fi router...
November 1, 2017: Journal of the Experimental Analysis of Behavior
https://www.readbyqxmd.com/read/29070625/individualized-tracking-of-self-directed-motor-learning-in-group-housed-mice-performing-a-skilled-lever-positioning-task-in-the-home-cage
#12
Gergely Silasi, Jamie Boyd, Federico Bolanos, Jeff LeDue, Stephen H Scott, Timothy H Murphy
Skilled forelimb function in mice is traditionally studied through behavioural paradigms that require extensive training by investigators, and are limited by the number of trials individual animals are able to perform within supervised session. We developed a skilled lever positioning task that mice can perform within their home cage. The task requires mice to use their forelimb to precisely hold a lever mounted on a rotary encoder within a rewarded position in order to dispense a water reward. A Raspberry Pi microcomputer is used to record lever position during trials, and to control task parameters, thus making this low-footprint apparatus ideal for use within animal housing facilities...
October 25, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29068672/effects-of-black-raspberry-extract-and-berry-compounds-on-repair-of-dna-damage-and-mutagenesis-induced-by-chemical-and-physical-agents-in-human-oral-leukoplakia-and-rat-oral-fibroblasts
#13
Joseph Guttenplan, Kun-Ming Chen, Yuan-Wan Sun, Braulio Lajara, Nora Ae Shalaby, Wieslawa Kosinska, Gabrielle Benitez, Krishne Gowda, Shantu Amin, Gary David Stoner, Karam El-Bayoumy
Black raspberries (BRB) have been shown to inhibit carcinogenesis in a number of systems, with most studies focusing on progression. Previously we reported that an anthocyanin-enriched black raspberry extract (BE) enhanced repair of dibenzo[a,l]pyrene dihydrodiol (DBP-diol) induced DNA adducts and inhibited DBP-diol and DBP-diolepoxide (DBPDE)-induced mutagenesis in a lacI rat oral fibroblast cell line, suggesting a role for BRB in the inhibition of initiation of carcinogenesis. Here we extend this work to protection by BE, against DNA adduct formation induced by dibenzo[a,l]pyrene (DBP) in a human oral leukoplakia cell line (MSK) and to second carcinogen, UV light...
October 25, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29060793/a-low-cost-video-oculography-system-for-vestibular-function-testing
#14
Jihwan Park, Youngsun Kong, Yunyoung Nam
In order to remain in focus during head movements, vestibular-ocular reflex causes eyes to move in the opposite direction to head movement. Disorders of vestibular system decrease vision, causing abnormal nystagmus and dizziness. To diagnose abnormal nystagmus, various studies have been reported including the use of rotating chair tests and videonystagmography. However, these tests are unsuitable for home use due to their high costs. Thus, a low-cost video-oculography system is necessary to obtain clinical features at home...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29055975/changes-in-the-level-and-antioxidant-activity-of-polyphenols-during-storage-of-enzymatically-treated-raspberry-juices-and-syrups
#15
Urszula Szymanowska, Barbara Baraniak, Urszula Gawlik-Dziki
BACKGROUND: Berry juices are a rich source of phenolic compounds exhibiting antioxidant activity. Unfortu- nately, polyphenols and especially anthocyanins are degraded during storage. METHODS: The levels of total phenolic compounds, phenolic acids, flavonoids, and antho- cyanins as well as antioxidant activity (radical scavenging ability against DPPH and ABTS+• and chelating power Fe2+) were determined in raspberry juices (obtained after enzymatic treatment with three commercial pectinolytic enzyme preparations) and syrups (obtained by the addition of sucrose at concentrations of 30% and 70%) during storage...
July 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29049280/ethoscopes-an-open-platform-for-high-throughput-ethomics
#16
Quentin Geissmann, Luis Garcia Rodriguez, Esteban J Beckwith, Alice S French, Arian R Jamasb, Giorgio F Gilestro
Here, we present the use of ethoscopes, which are machines for high-throughput analysis of behavior in Drosophila and other animals. Ethoscopes provide a software and hardware solution that is reproducible and easily scalable. They perform, in real-time, tracking and profiling of behavior by using a supervised machine learning algorithm, are able to deliver behaviorally triggered stimuli to flies in a feedback-loop mode, and are highly customizable and open source. Ethoscopes can be built easily by using 3D printing technology and rely on Raspberry Pi microcomputers and Arduino boards to provide affordable and flexible hardware...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/29045660/evaluation-of-off-season-potential-breeding-sources-for-spotted-wing-drosophila-drosophila-suzukii-matsumura-in-michigan
#17
Harit K Bal, Christopher Adams, Matthew Grieshop
It has been suggested that fruit wastes including dropped and unharvested fruits, and fruit byproducts (i.e., pomace) found in fruit plantings and cideries or wine-making facilities could serve as potential off-season breeding sites for spotted wing Drosophila (Drosophila suzukii Matsumura (Diptera: Drosophilidae)). This idea, however, has yet to be widely tested. The goal of our study was to determine the potential of dropped fruit and fruit wastes as Fall spotted wing Drosophila breeding resources in Michigan, USA...
October 16, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29042676/semiochemical-mediated-enhancement-of-males-to-complement-sterile-insect-technique-in-management-of-the-tephritid-pest-bactrocera-tryoni-froggatt
#18
Mohammed Abul Monjur Khan, Nicholas C Manoukis, Terry Osborne, Idris M Barchia, Geoff M Gurr, Olivia L Reynolds
Queensland fruit fly, Bactrocera tryoni (Froggatt), is the most significant pest of Australia's $9 billion horticulture industry. The sterile insect technique (SIT) and cue-lure (a synthetic analogue of raspberry ketone (RK))-based male annihilation technique (MAT) are two of the most effective management tools against this pest. However, combining these two approaches is considered incompatible as MAT kills sterile and 'wild' males indiscriminately. In the present study we tested the effect of pre-release feeding of B...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29030181/use-of-gelatin-and-gum-arabic-for-encapsulation-of-black-raspberry-anthocyanins-by-complex-coacervation
#19
Rezvan Shaddel, Javad Hesari, Sodeif Azadmard-Damirchi, Hamed Hamishehkar, Bahram Fathi-Achachlouei, Qingrong Huang
Protein-polysaccharide interactions offer opportunities for designing the new functional foods with applications in the food and pharmaceutical industries. In this work, we microencapsulated black raspberry water extracts by double emulsion technique prior to complex coacervation using gelatin and gum Arabic to diminish the instability of their anthocyanins (ANCs) as water soluble compounds, particularly under harsh processing and storage conditions. Subsequently, optical microscopy, moisture, hygroscopicity, solubility, particle size, loading capacity, zeta potential, color parameters (L*, a*, b*, C, H° and TCD), stability, Fourier transform infrared spectroscopy (FTIR), and thermal behavior were evaluated...
October 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29028333/profiling-and-quantification-of-regioisomeric-caffeoyl-glucoses-in-berry-fruits
#20
Maria Alexandra Patras, Nikolai Kuhnert, Rakesh Jaiswal, Gordon J McDougall
Based on a recently developed tandem MS based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected berry fruits were profiled for their caffeoyl glucose ester content. Fresh edible berries profiled included strawberries, raspberries, blueberries, blackberries, red and black currants, lingonberries, gooseberries, cranberries, juice of elderberries, goji berries, chokeberries, cranberries, açai berries, sea buckthorn berries, Montmorency sour cherries, and pomegranate were investigated...
October 13, 2017: Journal of Agricultural and Food Chemistry
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