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https://www.readbyqxmd.com/read/28548488/a-fluorescent-split-aptamer-for-visualizing-rna-rna-assembly-in-vivo
#1
Khalid K Alam, Kwaku D Tawiah, Matthew F Lichte, David Porciani, Donald H Burke
RNA-RNA assembly governs key biological processes and is a powerful tool for engineering synthetic genetic circuits. Characterizing RNA assembly in living cells often involves monitoring fluorescent reporter proteins, which are at best indirect measures of underlying RNA-RNA hybridization events and are subject to additional temporal and load constraints associated with translation and activation of reporter proteins. In contrast, RNA aptamers that sequester small molecule dyes and activate their fluorescence are increasingly utilized in genetically encoded strategies to report on RNA-level events...
May 26, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28545668/phenethyl-isothiocyanate-suppresses-cancer-stem-cell-properties-in-vitro-and-in-a-xenograft-model
#2
Ji Ho Yun, Kyung-A Kim, Gyhye Yoo, Sun Young Kim, Ji Min Shin, Jung Hoon Kim, Sang Hoon Jung, Jungho Kim, Chu Won Nho
BACKGROUND: Cancer stem cells (CSCs) are a subset of cells within the bulk of a tumor that have the ability to self-renew and differentiate, and are thus associated with cancer invasion, metastasis, and recurrence. Phenethyl isothiocyanate (PEITC) is a natural compound found in cruciferous vegetables such as broccoli and is used as a cancer chemopreventive agent; however, its effects on CSCs are little known. PURPOSE: To evaluate the effect of PEITC on CSCs in this study by examining CSC properties...
July 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28530603/modelling-of-the-effect-of-selenium-fertilization-on-the-content-of-bioactive-compounds-in-broccoli-heads
#3
Andrea Mahn
Selenium (Se) exerts many effects beneficial to health. Broccoli is a Se-hyperaccumulator plant, with Se-fertilization increasing its potential as a functional food. We studied the effect of dose, and the developmental stage at the beginning of Se-fortification, on antioxidant capacity, phenolics, glucosinolates, sulphoraphane, Se-methyl selenocysteine and myrosinase in broccoli. Se-fortification decreased the antioxidant properties and sulphur-containing compounds, but increased Se-methyl-selenocysteine content...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28528099/investigation-on-water-status-and-distribution-in-broccoli-and-the-effects-of-drying-on-water-status-using-nmr-and-mri-methods
#4
Fangfang Xu, Xin Jin, Lu Zhang, Xiao Dong Chen
Many quality attributes of food products are influenced by the water status and the microstructure. Low-field nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) methods are applied to non-destructively monitor the water status and structure of food. The aim of this study is to investigate the water status and distribution inside broccoli tissues and the effects of hot-air drying on the water status by using NMR and MRI methods. Transverse relaxation times (T2) provide the information of water status and water distribution...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28524727/e-cadherin-%C3%AE-catenin-complex-a-target-for-anticancer-and-antimetastasis-plants-plant-derived-compounds
#5
Majid Tafrihi, Roohollah Nakhaei Sistani
Plants reputed to have cancer-inhibiting potential and putative active components derived from those plants have emerged as an exciting new field in cancer study. Some of these compounds have cancer-inhibiting potential in different clinical staging levels, especially metastasis. A few of them which stabilize cell-cell adhesions are controversial topics. This review article introduces some effective herbal compounds that target E-cadherin/β-catenin protein complex. In this article, at first, we briefly review the structure and function of E-cadherin and β-catenin proteins, Wnt signaling pathway, and its target genes...
May 19, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28515063/learning-to-like-vegetables-during-breastfeeding-a-randomized-clinical-trial-of-lactating-mothers-and-infants
#6
Julie A Mennella, Loran M Daniels, Ashley R Reiter
Background: What lactating mothers eat flavors breast milk and, in turn, modifies their infants' acceptance of similarly flavored foods.Objective: We sought to determine the effects of the timing and duration of eating a variety of vegetables during breastfeeding on the liking of vegetables in both members of the dyad.Design: We conducted a randomized controlled study of 97 mother-infant dyads. Lactating mothers drank vegetable, beet, celery, and carrot juices for 1 mo beginning at 0.5, 1.5, or 2.5 mo postpartum or for 3 mo beginning at 0...
May 17, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28499433/servar-an-augmented-reality-tool-to-guide-the-serving-of-food
#7
Megan E Rollo, Tamara Bucher, Shamus P Smith, Clare E Collins
BACKGROUND: Accurate estimation of food portion size is a difficult task. Visual cues are important mediators of portion size and therefore technology-based aids may assist consumers when serving and estimating food portions. The current study evaluated the usability and impact on estimation error of standard food servings of a novel augmented reality food serving aid, ServAR. METHODS: Participants were randomised into one of three groups: 1) no information/aid (control); 2) verbal information on standard serving sizes; or 3) ServAR, an aid which overlayed virtual food servings over a plate using a tablet computer...
May 12, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28498473/sulforaphane-sensitizes-human-cholangiocarcinoma-to-cisplatin-via-the-downregulation-of-anti-apoptotic-proteins
#8
Rokas Račkauskas, Dachen Zhou, Simonas Ūselis, Kęstutis Strupas, Ingrid Herr, Peter Schemmer
Cholangiocarcinoma (CCC) is an aggressive malignancy with poor therapeutic options and pronounced chemotherapy resistance. The bioactive broccoli substance, sulforaphane (SFN), is a promising new therapeutic option since it has been found to induce therapeutic effects in both experimental and epidemiological studies in various tumor entities. Thus, the present study was designed to assess the effect of SFN on cisplatin sensitivity in CCC. Human HuCCT-1 and TFK-1 cells, representing intrahepatic and extrahepatic CCC, respectively, were treated with cisplatin and SFN...
May 3, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28490075/development-of-a-near-infrared-spectroscopy-method-nirs-for-fast-analysis-of-total-indolic-aliphatic-and-individual-glucosinolates-in-new-bred-open-pollinating-genotypes-of-broccoli-brassica-oleracea-convar-botrytis-var-italica
#9
Samira Sahamishirazi, Sabine Zikeli, Michael Fleck, Wilhelm Claupein, Simone Graeff-Hoenninger
This study describes the development of near-infrared spectroscopy (NIRS) calibration to determine individual and total glucosinolates (GSLs) content of 12 new-bred open-pollinating genotypes of broccoli (Brassica oleracea convar. botrytis var. italica). Six individual GSLs were identified using high-performance-liquid chromatography (HPLC). The NIRS calibration was established based on modified partial least squares regression with reference values of HPLC. The calibration was analyzed using coefficient of determination in prediction (R(2)) and ratio of preference of determination (RPD)...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28483571/sinigrin-attenuates-the-progression-of-atherosclerosis-in-apoe-mice-fed-a-high-cholesterol-diet-potentially-by-inhibiting-vcam-1-expression
#10
Yeon Jeong Jang, Bongkyun Park, Hee-Weon Lee, Hye Jin Park, Hyun Jung Koo, Byung Oh Kim, Eun-Hwa Sohn, Sung Hee Um, Suhkneung Pyo
Atherosclerosis is a complex inflammatory disease associated with elevated levels of atherogenic molecules for leukocyte recruitment. Sinigrin (2-propenylglucosinolate) is found mainly in broccoli, brussels sprouts, and black mustard seeds. Recently, sinigrin has received attention for its role in disease prevention and health promotion. In this study, we examined the effect of sinigrin on development of atherosclerosis in ApoE(-/-) mice and the expression of adhesion molecules in vascular smooth muscle cells (VSMCs)...
May 5, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28474472/analysis-of-brassica-oleracea-early-stage-abiotic-stress-responses-reveals-tolerance-in-multiple-crop-types-and-for-multiple-sources-of-stress
#11
Andrew M Beacham, Paul Hand, David A C Pink, James M Monaghan
BACKGROUND: Brassica oleracea includes a number of important crop types such as cabbage, cauliflower, broccoli and kale. Current climate conditions and weather patterns are causing significant losses in these crops, meaning that new cultivars with improved tolerance of one or more abiotic stress types must be sought. We assayed genetically fixed B. oleracea lines belonging to a Diversity Fixed Foundation Set (DFFS) for their response to seedling stage-imposed drought, flood, salinity, heat and cold stress...
May 5, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28472782/identification-of-deubiquitinase-targets-of-isothiocyanates-using-silac-assisted-quantitative-mass-spectrometry
#12
Ann P Lawson, Daniel W Bak, D Alexander Shannon, Marcus J C Long, Tushara Vijaykumar, Runhan Yu, Farid El Oualid, Eranthie Weerapana, Lizbeth Hedstrom
Cruciferous vegetables such as broccoli and kale have well documented chemopreventative and anticancer effects that are attributed to the presence of isothiocyanates (ITCs). ITCs modulate the levels of many oncogenic proteins, but the molecular mechanisms of ITC action are not understood. We previously reported that phenethyl isothiocyanate (PEITC) inhibits two deubiquitinases (DUBs), USP9x and UCH37. DUBs regulate many cellular processes and DUB dysregulation is linked to the pathogenesis of human diseases including cancer, neurodegeneration, and inflammation...
April 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28472642/influence-of-seasoning-on-vegetable-selection-liking-and-intent-to-purchase
#13
Joanna Manero, Carter Phillips, Brenna Ellison, Soo-Yeun Lee, Sharon M Nickols-Richardson, Karen M Chapman-Novakofski
Low vegetable intake continues to be a health concern, and strategies to increase vegetable intake have resulted in only small increases. One strategy that has received less attention is the use of seasonings. This study's objective was to determine the impact of seasoning on vegetable selection, liking, and intent to purchase. We conducted a 3-week study in a public café on a university campus. Customers buying a main dish could select a vegetable side (seasoned [SS] or steamed [ST]) at no cost. Based on café data and power analysis (alpha 0...
May 1, 2017: Appetite
https://www.readbyqxmd.com/read/28471020/kinetic-parameters-for-the-thermal-inactivation-of-peroxidase-and-lipoxygenase-in-precooked-frozen-brassica-species
#14
John Pérez-Calderón, Alicia Califano, María Victoria Santos, Noemí Zaritzky
Thermal inactivation of peroxidase (POD) and lipoxygenase (LOX), both enzymes present in broccoli and Brussels sprouts, is required before freezing, to obtain high-quality precooked frozen vegetables. Rate constants of a 1st-order biphasic model for the heat-labile and heat-resistant POD and LOX isoenzymes were determined at different temperatures (75, 80, and 90 °C) and the corresponding activation energies were estimated using nonlinear regressions. In the case of Brussels sprouts, the activation energies for the resistant and labile fractions were 56...
May 4, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28467185/copper-and-zinc-tolerance-in-bacteria-isolated-from-fresh-produce
#15
Ismael Cidre, Rubén Pérez Pulido, Maria José Grande Burgos, Antonio Gálvez, Rosario Lucas
The continued agricultural exposure of bacteria to metals such as copper and zinc may result in an increased copper tolerance through the food chain. The aim of this study was to determine the Cu and Zn tolerance of bacteria from fresh produce (cucumber, zucchini, green pepper, tomato, lettuce, vegetable salad, broccoli, cabbage, carrot, green onion, onion, and mango). Isolates (506 aerobic mesophiles) from 12 different food produce products were tested for growth in a range of Cu and Zn concentrations. Selected isolates were identified using 16S rDNA sequencing, and the presence of metal resistance genes was studied using PCR amplification...
May 3, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28420123/stability-of-bioactive-compounds-in-broccoli-as-affected-by-cutting-styles-and-storage-time
#16
Ana Mariel Torres-Contreras, Vimal Nair, Luis Cisneros-Zevallos, Daniel A Jacobo-Velázquez
Broccoli contains bioactive molecules and thus its consumption is related with the prevention of chronic and degenerative diseases. The application of wounding stress to horticultural crops is a common practice, since it is the basis for the fresh-cut produce industry. In this study, the effect of four different cutting styles (CSs) (florets (CS1), florets cut into two even pieces (CS2), florets cut into four even pieces (CS3), and florets processed into chops (CS4)) and storage time (0 and 24 h at 20 °C) on the content of bioactive compounds in broccoli was evaluated...
April 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28419960/preferentially-enhancing-anti-cancer-isothiocyanates-over-glucosinolates-in-broccoli-sprouts-how-nacl-and-salicylic-acid-affect-their-formation
#17
Azadeh Esfandiari, Ali Saei, Marian J McKenzie, Adam J Matich, Mesbah Babalar, Donald A Hunter
Broccoli (Brassica oleracea L. var. italica) sprouts contain glucosinolates (GLs) that when hydrolysed yield health promoting isothiocyanates such as sulforaphane (SF). SF content can be increased by salt (NaCl) stress, although high salt concentrations negatively impact plant growth. Salicylic acid (SA) treatments can attenuate the negative effects of salt on growth. To test whether sprout isothiocyanate content could be elevated without sprout growth being compromised, broccoli seed were germinated and grown for seven days in salt (0, 80 and 160 mM) alone and in combination with 100 μM SA...
April 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28415982/the-treatment-of-primary-mediastinal-large-b-cell-lymphoma-a-two-decades-monocentric-experience-with-98-patients
#18
Alessandro Broccoli, Beatrice Casadei, Vittorio Stefoni, Cinzia Pellegrini, Federica Quirini, Lorenzo Tonialini, Alice Morigi, Miriam Marangon, Lisa Argnani, Pier Luigi Zinzani
BACKGROUND: The purpose of this study is to investigate the most suitable first-line approach and the best combination treatment for primary mediastinal large B-cell lymphoma (PMLBCL) as they have been matter of debate for at least two decades. METHODS: Our single centre experience in the treatment of 98 de novo PMLBCL patients over the last 20 years is reviewed. All patients received MACOP-B chemotherapy. Thirty-seven received both rituximab and mediastinal radiotherapy; 30 were irradiated after chemotherapy, although not receiving rituximab and 20 received rituximab without radiotherapy consolidation...
April 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28400006/microbiological-quality-of-selected-ready-to-eat-leaf-vegetables-sprouts-and-non-pasteurized-fresh-fruit-vegetable-juices-including-the-presence-of-cronobacter-spp
#19
Anna Berthold-Pluta, Monika Garbowska, Ilona Stefańska, Antoni Pluta
Bacteria of the genus Cronobacter are emerging food-borne pathogens. Foods contaminated with Cronobacter spp. may pose a risk to infants or adults with suppressed immunity. This study was aimed at determining the microbiological quality of ready-to-eat (RTE) plant-origin food products available on the Polish market with special emphasis on the prevalence of Cronobacter genus bacteria. Analyses were carried out on 60 samples of commercial RTE type plant-origin food products, including: leaf vegetables (20 samples), sprouts (20 samples) and non-pasteurized vegetable, fruit and fruit-vegetable juices (20 samples)...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28394276/targeting-colorectal-cancer-proliferation-stemness-and-metastatic-potential-using-brassicaceae-extracts-enriched-in-isothiocyanates-a-3d-cell-model-based-study
#20
Lucília P Pereira, Patrícia Silva, Marlene Duarte, Liliana Rodrigues, Catarina M M Duarte, Cristina Albuquerque, Ana Teresa Serra
Colorectal cancer (CRC) recurrence is often attributable to circulating tumor cells and/or cancer stem cells (CSCs) that resist to conventional therapies and foster tumor progression. Isothiocyanates (ITCs) derived from Brassicaceae vegetables have demonstrated anticancer effects in CRC, however little is known about their effect in CSCs and tumor initiation properties. Here we examined the effect of ITCs-enriched Brassicaceae extracts derived from watercress and broccoli in cell proliferation, CSC phenotype and metastasis using a previously developed three-dimensional HT29 cell model with CSC-like traits...
April 10, 2017: Nutrients
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