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https://www.readbyqxmd.com/read/29149798/reconstructing-biosynthetic-pathway-of-the-plant-derived-cancer-chemopreventive-precursor-glucoraphanin-in-escherichia-coli
#1
Han Yang, Feixia Liu, Yin Li, Bo Yu
Epidemiological data confirmed a strong correlation between regular consumption of cruciferous vegetables and lower cancer risk. This cancer preventive property is mainly attributed to the glucosinolate products, such as glucoraphanin found in broccoli that is derived from methionine. Here we report the first successful reconstruction of the complete biosynthetic pathway of glucoraphanin from methionine in Escherichia coli via gene selection, pathway design, and protein engineering. We used branched-chain amino transferase 3 to catalyze two transamination steps to ensure the purity of precursor molecules and used cysteine as a sulfur donor to simplify the synthesis pathway...
November 17, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29147630/sulforaphane-from-broccoli-reduces-symptoms-of-autism-a-follow-up-case-series-from-a-randomized-double-blind-study
#2
Rhoda Lynch, Eileen L Diggins, Susan L Connors, Andrew W Zimmerman, Kanwaljit Singh, Hua Liu, Paul Talalay, Jed W Fahey
Introduction: Autism spectrum disorder (ASD) affects 1 in 68 children, is characterized by impaired social interaction and communication as well as restricted or repetitive behaviors, and varies widely with respect to its causes and presentations. There are no validated pharmacologic treatments for the core symptoms of ASD. The social, medical, and economic burdens of ASD on families and caregivers are profound. We recently showed in a small clinical trial that sulforaphane (SF) from broccoli sprouts could significantly reduce the behavioral symptoms of ASD...
2017: Global Advances in Health and Medicine: Improving Healthcare Outcomes Worldwide
https://www.readbyqxmd.com/read/29138618/diversity-phylogeny-and-host-specialization-of-hyaloperonospora-species-in-korea
#3
Jae Sung Lee, Hyang Burm Lee, Hyeon-Dong Shin, Young-Joon Choi
The genus Hyaloperonospora (Peronosporaceae; Oomycota) is an obligate biotrophic group that causes downy mildew disease on the Brassicaceae and allied families of Brassicales, including many economically relevant crops, such as broccoli, cabbage, radish, rape, and wasabi. To investigate the diversity of Hyaloperonospora species in northeast Asia, we performed a morphological analysis for the dried herbarium specimens collected in Korea, along with molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mtDNA sequences...
September 2017: Mycobiology
https://www.readbyqxmd.com/read/29126060/selenium-treatment-differentially-affects-sulfur-metabolism-in-high-and-low-glucosinolate-producing-cultivars-of-broccoli-brassica-oleracea-l
#4
Marian J McKenzie, Ronan K Y Chen, Susanna Leung, Srishti Joshi, Paula E Rippon, Nigel I Joyce, Michael T McManus
The effect of selenium (Se) application on the sulfur (S)-rich glucosinolate (GSL)-containing plant, broccoli (Brassica oleracea L. var. italica) was examined with a view to producing germplasm with increased Se and GSL content for human health, and to understanding the influence of Se on the regulation of GSL production. Two cultivars differing in GSL content were compared. Increased Se application resulted in an increase in Se uptake in planta, but no significant change in total S or total GSL content in either cultivar...
October 31, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29121206/effects-of-temperatures-on-immature-development-and-survival-of-the-invasive-stink-bug-hemiptera-pentatomidae
#5
Darcy A Reed, Fatemeh Ganjisaffar, John C Palumbo, Thomas M Perring
Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae) is a non-native stink bug that feeds primarily on cole crops and wild mustards. Its invasion into desert agriculture in California and Arizona presents a conundrum between rapid pest development at warm temperatures and severe damage to cool season crops. In this study, the development and survival of B. hilaris were determined at nine constant temperatures (ranging from 20-42°C) when reared on organically grown broccoli florets. Egg hatching was greatly delayed at 20°C, and first instar nymphs did not survive at this temperature...
November 7, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29120785/identification-and-characterization-of-pesticide-metabolites-in-brassica-species-by-liquid-chromatography-travelling-wave-ion-mobility-quadrupole-time-of-flight-mass-spectrometry-uplc-twims-qtof-ms
#6
Anna Bauer, Jens Luetjohann, Franziska S Hanschen, Monika Schreiner, Jürgen Kuballa, Eckard Jantzen, Sascha Rohn
A new mass spectrometric method for evaluating metabolite formation of the pesticides thiacloprid, azoxystrobin, and difenoconazole was developed for the Brassica species pak choi and broccoli. Both, distribution and transformation kinetics of the active compounds and their metabolites were analyzed by UPLC-TWIMS-QTOF-MS. Additionally, HR-MS analysis and structure elucidation tools such as diagnostic ions, isotopic matches, and collision cross sections were applied for metabolites identification. Following the application of two plant protection products (containing the above-mentioned active compounds) in a greenhouse study plant material was cryo-milled and extracted with water/methanol...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29113068/uva-uvb-light-and-methyl-jasmonate-alone-or-combined-redirect-the-biosynthesis-of-glucosinolates-phenolics-carotenoids-and-chlorophylls-in-broccoli-sprouts
#7
Melissa Moreira-Rodríguez, Vimal Nair, Jorge Benavides, Luis Cisneros-Zevallos, Daniel A Jacobo-Velázquez
Broccoli sprouts contain health-promoting phytochemicals that can be enhanced by applying ultraviolet light (UV) or phytohormones. The separate and combined effects of methyl jasmonate (MJ), UVA, or UVB lights on glucosinolate, phenolic, carotenoid, and chlorophyll profiles were assessed in broccoli sprouts. Seven-day-old broccoli sprouts were exposed to UVA (9.47 W/m²) or UVB (7.16 W/m²) radiation for 120 min alone or in combination with a 25 µM MJ solution, also applied to sprouts without UV supplementation...
November 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29112318/antimicrobial-activity-of-broccoli-brassica-oleracea-var-italica-cultivar-avenger-against-pathogenic-bacteria-phytopathogenic-filamentous-fungi-and-yeast
#8
R D Pacheco-Cano, R Salcedo-Hernández, J E López-Meza, D K Bideshi, J E Barboza-Corona
AIMS: The objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. METHODS AND RESULTS: Crude extracts were obtained from florets and stems of broccoli cultivar Avenger and the inhibitory effect was demonstrated against pathogenic bacteria (Bacillus cereus, Staphylococcus xylosus, S...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29108741/the-role-of-smell-taste-flavour-and-texture-cues-in-the-identification-of-vegetables
#9
V L van Stokkom, A E Blok, O van Kooten, C de Graaf, M Stieger
It has been shown that the identification of many foods including vegetables based on flavour cues is often difficult. The effect of providing texture cues in addition to flavour cues on the identification of foods and the effect of providing taste cues only on the identification of foods have not been studied. The aim of this study was to assess the role of smell, taste, flavour and texture cues in the identification of ten vegetables commonly consumed in The Netherlands (broccoli, cauliflower, French bean, leek, bell pepper, carrot, cucumber, iceberg lettuce, onion and tomato)...
November 8, 2017: Appetite
https://www.readbyqxmd.com/read/29102679/cutaneous-t-cell-lymphomas-focusing-on-novel-agents-in-relapsed-and-refractory-disease
#10
REVIEW
Lisa Argnani, Alessandro Broccoli, Pier Luigi Zinzani
Patients with relapsed or refractory cutaneous T-cell lymphoma (CTCL) display a dismal prognosis and their therapy represents an unmet medical need, as the best treatment strategy is yet to be determined. Exciting data on novel targeted agents are now emerging from recently concluded and ongoing clinical trials in patients with relapsed and refractory CTCL. Three FDA approved compounds are used as single agents including the oral retinoid bexarotene and histone deacetylase inhibitors romidepsin and vorinostat...
December 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/29089926/impact-of-synergistic-association-of-zno-nanorods-and-symbiotic-fungus-piriformospora-indica-dsm-11827-on-brassica-oleracea-var-botrytis-broccoli
#11
Uma Singhal, Manika Khanuja, Ram Prasad, Ajit Varma
In the present work, novel nanotool called 'nano-embedded fungus' formed by impact of synergistic association of ZnO-nanorods and fungus Piriformospora indica DSM 11827, for growth of Brassica oleracea var. botrytis (Broccoli) is reported. ZnO-nanorods were synthesized by mechanical assisted thermal decomposition process and characterized by scanning electron microscopy (SEM) for morphology, X-ray diffraction for structural studies and UV-vis absorption spectroscopy for band gap determination. Nanoembedded fungus is prepared by optimizing ZnO-nanorods concentration (500 ppm) which resulted in the increased biomass of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29084128/the-effects-of-improving-the-mesothelioma-surveillance-network-on-sensitivity-timeliness-in-reporting-and-asbestos-exposure-assessment
#12
Lucia Mangone, Enza Di Felice, Cinzia Storchi, Antonio Romanelli, Serena Broccoli, Massimo Vicentini, Paolo Giorgi Rossi
BACKGROUND: In Italy, Mesothelioma Registries (MRs) have been established by law for the epidemiological surveillance of occupational cancers. MRs collect information about asbestos exposure of incident cases, through interviews. In the Emilia-Romagna region, MR was implemented in 1996 and extended its network of health professionals who report suspected mesothelioma in 2001 and 2007. OBJECTIVES: This study evaluated the impact of the extension of the network on MR sensitivity and timeliness...
October 27, 2017: La Medicina del Lavoro
https://www.readbyqxmd.com/read/29080462/effect-of-broccoli-sprouts-on-thyroid-function-haematological-biochemical-and-immunological-parameters-in-rats-with-thyroid-imbalance
#13
Paweł Paśko, Mirosław Krośniak, Ewelina Prochownik, Małgorzata Tyszka-Czochara, Maria Fołta, Renata Francik, Jakub Sikora, Mateusz Malinowski, Paweł Zagrodzki
Broccoli sprouts may exert a negative influence on thyroid function as they are a rich source of glucosinolates, in particular glucoraphanin. Under the study in a long-term experiment broccoli sprouts were tested as an element of rats diet, combined with deficient iodine, or sulfadimethoxine ingestion - two models of hypothyroidism. Evaluations were performed for serum TSH and thyroid hormones completed with analyzes of selected haematological, biochemical and immunological (IL-6, IL-10) parameters, as well as cytosolic glutathione peroxidase (GPX1), thioredoxin reductase (TR) in the thyroid, and plasma glutathione peroxidase (GPX3)...
October 25, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29069139/elemental-composition-of-vegetables-cultivated-over-coal-mining-waste
#14
Jairo J Zocche, Paula Rohr, Adriani P Damiani, Daniela D Leffa, Miriam C Martins, Caroline M Zocche, Karina O Teixeira, Gabriela D Borges, Maiellen M DE Jesus, Carla E I Dos Santos, Johnny F Dias, Vanessa M DE Andrade
We assessed elemental composition of the liver in mice subjected to one-time or chronic consumption of the juice of vegetables cultivated in a vegetable garden built over deposits of coal waste. Lactuca sativa L. (lettuce), Beta vulgaris L. (beet), Brassica oleracea L. var. italica (broccoli) and Brassica oleracea L. var. acephala (kale) were collected from the coal-mining area and from a certified organic farm (control). Elemental composition was analyzed by particle-induced X-ray emission (PIXE) method. Concentrations of Mg, S, and Ca of mice subjected to one-time consumption of broccoli and concentrations of these same elements plus Si of mice receiving kale were higher in the coal-mining area...
October 23, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29058634/three-dimensional-ultrastructure-of-capillary-endothelial-glycocalyx-under-normal-and-experimental-endotoxemic-conditions
#15
Hideshi Okada, Genzou Takemura, Kodai Suzuki, Kazumasa Oda, Chihiro Takada, Yasuaki Hotta, Nagisa Miyazaki, Akiko Tsujimoto, Isamu Muraki, Yoshiaki Ando, Ryogen Zaikokuji, Atsumu Matsumoto, Hiroki Kitagaki, Yuto Tamaoki, Takahiro Usui, Tomoaki Doi, Takahiro Yoshida, Shozo Yoshida, Hiroaki Ushikoshi, Izumi Toyoda, Shinji Ogura
BACKGROUND: Sugar-protein glycocalyx coats healthy endothelium, but its ultrastructure is not well described. Our aim was to determine the three-dimensional ultrastructure of capillary endothelial glycocalyx in the heart, kidney, and liver, where capillaries are, respectively, continuous, fenestrated, and sinusoidal. METHODS: Tissue samples were processed with lanthanum-containing alkaline fixative, which preserves the structure of glycocalyx. RESULTS: Scanning and transmission electron microscopy revealed that the endothelial glycocalyx layer in continuous and fenestrated capillaries was substantially thicker than in sinusoids...
October 23, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29043603/epigenetic-modifications-of-gene-expression-by-lifestyle-and-environment
#16
REVIEW
Qudeer Ahmed Abdul, Byung Pal Yu, Hae Young Chung, Hyun Ah Jung, Jae Sue Choi
Epigenetics oftenly described as the heritable changes in gene expression independent of changes in DNA sequence. Various environmental factors such as nutrition-dietary components, lifestyle, exercise, physical activity, toxins, and other contributing factors remodel the genome either in a constructive or detrimental way. Since epigenetic changes are reversible and nutrition is one of the many epigenetic regulators that modify gene expression without changing the DNA sequence, dietary nutrients and bioactive food components contribute to epigenetic phenomena either by directly suppressing DNA methylation or histone catalyzing enzymes or by changing the availability of substrates required for enzymatic reactions...
October 17, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/29037688/production-of-phytotherapeutics-from-broccoli-juice-by-integrated-membrane-processes
#17
Emel Yilmaz, Pelin Onsekizoglu Bagci
An integrated membrane process for the recovery and concentration of phytotherapeutics in broccoli juice was investigated to develop a natural product that could be of interest for food and/or pharmaceutical industry. Following a pretreatment step to remove suspended solids, the juice was ultrafiltered through a 50kDa MWCO polyethersulfone membrane. The permeate stream was further concentrated by osmotic distillation up to 42.6°Brix. During ultrafiltration, a complete recovery of sulforaphane, malic acid and citric acid was achieved, where total phenolic content and ascorbic acid were recovered at a ratio of 94...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29021502/structural-analysis-of-an-innate-immunostimulant-from-broccoli-brassica-oleracea-var-italica
#18
Makoto Urai, Keiko Kataoka, Satoshi Nishida, Kazuhisa Sekimizu
Vegetables are eaten as part of a healthy diet throughout the world, and some are also applied topically as a traditional medicine. We evaluated the innate immunostimulating activities of hot water extracts of various vegetables using the silkworm muscle contraction assay system, and found that broccoli, Brassica oleracea var. italica, contains a strong innate immunostimulant. We purified the innate immunostimulant from broccoli, and characterized the chemical structure by chemical analyses and NMR spectroscopy...
October 11, 2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28991414/in-situ-spatial-complementation-of-aptamer-mediated-recognition-enables-live-cell-imaging-of-native-rna-transcripts-in-real-time
#19
Zejun Wang, Yao Luo, Xiaodong Xie, Xingjie Hu, Haiyun Song, Yun Zhao, Jiye Shi, Lihua Wang, Gennadi Glinsky, Nan Chen, Ratnesh Lal, Chunhai Fan
Direct cellular imaging of the localization and dynamics of biomolecules helps to understand their functions and reveals novel mechanisms at the single-cell resolution. Here, we report the development of an aptamer-initiated fluorescence complementation (AiFC) method for RNA imaging by engineering a green fluorescence protein (GFP)-mimicking turn-on RNA aptamer, Broccoli, into two split fragments that could tandemly bind to target mRNA. When genetically encoded in cells, nascent mRNA molecules recruited split Broccoli and brought the two fragments to the spatial proximity, which in-situ formed a fluorophore-binding site and turned on fluorescence...
October 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28983192/the-role-of-polyphenols-in-modern-nutrition
#20
REVIEW
G Williamson
Polyphenols are found in plant-based foods and beverages, notably apples, berries, citrus fruit, plums, broccoli, cocoa, tea and coffee and many others. There is substantial epidemiological evidence that a diet high in polyphenol-rich fruit, vegetables, cocoa and beverages protects against developing cardiovascular disease and type 2 diabetes. The absorption and metabolism of these compounds have been well described and, for many, the gut microbiota play a critical role in absorption; taking into consideration the parent compound and the metabolites from colon bacteria catabolism, more than 80% of a dose can be absorbed and ultimately excreted in the urine...
September 2017: Nutrition Bulletin
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