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https://www.readbyqxmd.com/read/28929757/high-pressure-processing-of-broccoli-sprouts-influence-on-bioactivation-of-glucosinolates-to-isothiocyanates
#1
Anna Westphal, Kenneth M Riedl, Jessica L Cooperstone, Shreya Kamat, V M Balasubramaniam, Steven J Schwartz, Volker Böhm
Effects of high-pressure processing (HPP, 100-600 MPa for 3 min at 30 °C) on the glucosinolate content, conversion to isothiocyanates, and color changes during storage in fresh broccoli sprouts were investigated. A mild heat treatment (60 °C) and boiling (100 °C) were used as positive and negative controls, respectively. Glucosinolates were quantified using liquid chromatography-mass spectrometry, and isothiocyanates were quantified using high-performance liquid chromatography-photodiode array detection...
September 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28917362/a-comparative-study-of-the-antacid-effect-of-some-commonly-consumed-foods-for-hyperacidity-in-an-artificial-stomach-model
#2
Vandana Panda, Priyanka Shinde, Jyoti Deora, Pankaj Gupta
OBJECTIVES: The incorporation of certain alkalinizing vegetables, fruits, milk and its products in the diet has been known to alleviate hyperacidity. These foods help to restore the natural gastric balance and function, curb acid reflux, aid digestion, reduce the burning sensation due to hyperacidity and soothe the inflamed mucosa of the stomach. The present study evaluates and compares the antacid effect of broccoli, kale, radish, cucumber, lemon juice, cold milk and curd in an artificial stomach model...
October 2017: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/28886456/broccoli-shaped-biosensor-hierarchy-for-electrochemical-screening-of-noradrenaline-in-living-cells
#3
Mohammed Y Emran, Moataz Mekawy, Naeem Akhtar, Mohamed A Shenashen, Islam M El-Sewify, Ahmed Faheem, Sherif A El-Safty
Monitoring and determination of ultra-trace concentrations of monoamine neurotransmitter such as noradrenaline (NA) in living cells with simple, sensitive and selective assays are significantly interesting. We design NA-electrode sensing system based on C-, N-doped NiO broccoli-like hierarchy (CNNB). The spherical broccoli-head umbrella architectures associated with nano-tubular arrangements enabled to tailor NA biosensor design. The homogenous doping and anisotropic dispersion of CN nanospheres along the entire NB head nanotubes lead to creating of abundant electroactive sites in the interior tubular vessels and outer surfaces for ultrasensitive detection of NA in living cells such as PC12...
August 31, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28882452/aav-php-b-mediated-global-scale-expression-in-the-mouse-nervous-system-enables-gba1-gene-therapy-for-wide-protection-from-synucleinopathy
#4
Giuseppe Morabito, Serena G Giannelli, Gabriele Ordazzo, Simone Bido, Valerio Castoldi, Marzia Indrigo, Tommaso Cabassi, Stefano Cattaneo, Mirko Luoni, Cinzia Cancellieri, Alessandro Sessa, Marco Bacigaluppi, Stefano Taverna, Letizia Leocani, José L Lanciego, Vania Broccoli
The lack of technology for direct global-scale targeting of the adult mouse nervous system has hindered research on brain processing and dysfunctions. Currently, gene transfer is normally achieved by intraparenchymal viral injections, but these injections target a restricted brain area. Herein, we demonstrated that intravenous delivery of adeno-associated virus (AAV)-PHP.B viral particles permeated and diffused throughout the neural parenchyma, targeting both the central and the peripheral nervous system in a global pattern...
August 10, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28881649/identification-of-deubiquitinase-targets-of-isothiocyanates-using-silac-assisted-quantitative-mass-spectrometry
#5
Ann P Lawson, Daniel W Bak, D Alexander Shannon, Marcus J C Long, Tushara Vijaykumar, Runhan Yu, Farid El Oualid, Eranthie Weerapana, Lizbeth Hedstrom
Cruciferous vegetables such as broccoli and kale have well documented chemopreventative and anticancer effects that are attributed to the presence of isothiocyanates (ITCs). ITCs modulate the levels of many oncogenic proteins, but the molecular mechanisms of ITC action are not understood. We previously reported that phenethyl isothiocyanate (PEITC) inhibits two deubiquitinases (DUBs), USP9x and UCH37. DUBs regulate many cellular processes and DUB dysregulation is linked to the pathogenesis of human diseases including cancer, neurodegeneration, and inflammation...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28877584/influence-of-modified-atmosphere-on-induction-and-activity-of-respiratory-enzymes-in-broccoli-florets-during-the-early-stage-of-postharvest-storage
#6
Hsiao-Wen Wang, Yoshio Makino, Jun Inoue, Kensaku Maejima, Sachiko Funayama-Noguchi, Takeshi Yamada, Ko Noguchi
Modified atmosphere packaging and controlled atmosphere storage (hypoxia conditions) extend shelf lives of horticultural products by depressing O2 uptake rate. We investigated the relationship between atmospheres and alternative oxidase (AOX) to cytochrome c oxidase (COX) activities (based on oxygen isotope discrimination) and relative amounts of two respiratory enzymes-AOX and COX-during the early stage of storage. Broccoli florets, with high O2 uptake rates, were stored under hypoxia and normoxia at 25°C...
September 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28876209/soil-microbiomes-associated-with-verticillium-wilt-suppressive-broccoli-and-chitin-amendments-are-enriched-with-potential-biocontrol-agents
#7
Patrik Inderbitzin, Judson Ward, Alexandra Barbella, Natalie Solares, Dmitriy Izyumin, Prabir Burman, Dan Chellemi, Krishna V Subbarao
Two naturally infested Verticillium wilt conducive soils from the Salinas Valley of coastal California were amended with disease-suppressive broccoli residue and/or crab meal amendments, and changes to the soil prokaryote community were monitored using Illumina sequencing of a 16S rRNA gene library generated from 160 bulk soil samples. The experiment was run in a greenhouse twice with eggplant as the Verticillium wilt susceptible host. Disease suppression, plant height, soil microsclerotia density and soil chitinase activity were assessed at the conclusion of each experiment...
September 6, 2017: Phytopathology
https://www.readbyqxmd.com/read/28873713/bioavailability-and-new-biomarkers-of-cruciferous-sprouts-consumption
#8
Nieves Baenas, Clara Suárez-Martínez, Cristina García-Viguera, Diego A Moreno
The evaluation of the bioavailability of bioactive compounds from cruciferous foods is one challenge in the design of clinical trials for studying their functionality. Currently, studies of bioavailability are mainly based of the analysis of total isothiocyanates and indoles, and sulforaphane metabolites after broccoli consumption. However, as far as we are aware, there are not any biomarkers studied or established for the intake of radish sprouts. In this work, a 7-days-cross-over study with fourteen women was undertaken to compare the bioavailability of glucosinolates from broccoli and radish sprouts...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28868057/selenium-induced-toxicity-is-counteracted-by-sulfur-in-broccoli-brassica-oleracea-l-var-italica
#9
Ming Tian, Maixia Hui, Theodore W Thannhauser, Siyi Pan, Li Li
Selenium (Se) is an essential micronutrient for humans. Increasing Se content in food crops offers an effective approach to enhance the consumption of Se in human diets. A thoroughly understanding of the effects of Se on plant growth is important for Se biofortification in food crops. Given that Se is an analog of sulfur (S) and can be toxic to plants, its effect on plant growth is expected to be greatly affected by S nutrition. However, this remains to be further understood. Here, we evaluated the influence of Se treatments on broccoli (Brassica oleracea L...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28862664/brassica-derived-plant-bioactives-as-modulators-of-chemopreventive-and-inflammatory-signaling-pathways
#10
REVIEW
Christine Sturm, Anika E Wagner
A high consumption of vegetables belonging to the Brassicaceae family has been related to a lower incidence of chronic diseases including different kinds of cancer. These beneficial effects of, e.g., broccoli, cabbage or rocket (arugula) intake have been mainly dedicated to the sulfur-containing glucosinolates (GLSs)-secondary plant compounds nearly exclusively present in Brassicaceae-and in particular to their bioactive breakdown products including isothiocyanates (ITCs). Overall, the current literature indicate that selected Brassica-derived ITCs exhibit health-promoting effects in vitro, as well as in laboratory mice in vivo...
September 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28846029/ayurvedic-preparation-of-zingiber-officinale-roscoe-effects-on-cardiac-and-on-smooth-muscle-parameters
#11
Alberto Leoni, Roberta Budriesi, Ferruccio Poli, Mariacaterina Lianza, Alessandra Graziadio, Alice Venturini, Massimiliano Broccoli, Matteo Micucci
The rhizome of the Zingiber officinale Roscoe, a biennial herb growing in South Asia, is commonly known as ginger. Ginger is used in clinical disorders, such as constipation, dyspepsia, diarrhoea, nausea and vomiting and its use is also recommended by the traditional medicine for cardiopathy, high blood pressure, palpitations and as a vasodilator to improve the circulation. The decoction of ginger rhizome is widely used in Ayurvedic medicine. In this papery by high-performance liquid chromatography, we have seen that its main phytomarkers were 6-gingerol, 8-gingerol and 6-shogaol and we report the effects of the decoction of ginger rhizome on cardiovascular parameters and on vascular and intestinal smooth muscle...
August 28, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28843124/impact-of-the-reusing-of-food-manufacturing-wastewater-for-irrigation-in-a-closed-system-on-the-microbiological-quality-of-the-food-crops
#12
Luciano Beneduce, Giuseppe Gatta, Antonio Bevilacqua, Angela Libutti, Emanuele Tarantino, Micol Bellucci, Eleonora Troiano, Giuseppe Spano
In order to evaluate if the reuse of food industry treated wastewater is compatible for irrigation of food crops, without increased health risk, in the present study a cropping system, in which ground water and treated wastewater were used for irrigation of tomato and broccoli, during consecutive crop seasons was monitored. Water, crop environment and final products were monitored for microbial indicators and pathogenic bacteria, by conventional and molecular methods. The microbial quality of the irrigation waters influenced sporadically the presence of microbial indicators in soil...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28839265/combinatorial-bioactive-botanicals-re-sensitize-tamoxifen-treatment-in-er-negative-breast-cancer-via-epigenetic-reactivation-of-er%C3%AE-expression
#13
Yuanyuan Li, Syed M Meeran, Trygve O Tollefsbol
Conventional cancer prevention has primarily focused on single chemopreventive compounds that may not be sufficiently efficacious. We sought to investigate potential combinatorial effects of epigenetic bioactive botanicals including epigallocatechin-3-gallate (EGCG) in green tea polyphenols (GTPs) and sulforaphane (SFN) in broccoli sprouts (BSp) on neutralizing epigenetic aberrations in estrogen receptor-α (ERα) leading to enhanced anti-hormone therapeutic efficacy in ERα-negative breast cancer. Our results showed that this combinatorial treatment re-sensitized ERα-dependent cellular inhibitory responses to an estrogen antagonist, tamoxifen (TAM), via at least in part, epigenetic reactivation of ERα expression in ERα-negative breast cancer cells...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28830206/sulforaphane-reduces-hmgb1-mediated-septic-responses-and-improves-survival-rate-in-septic-mice
#14
In-Chul Lee, Dae Yong Kim, Jong-Sup Bae
Sulforaphane (SFN), a natural isothiocyanate present in cruciferous vegetables such as broccoli and cabbage, is effective in preventing carcinogenesis, diabetes, and inflammatory responses. Inhibition of high mobility group box 1 (HMGB1) and restoration of endothelial integrity is emerging as an attractive therapeutic strategy in the management of severe sepsis or septic shock. In this study, we examined the effects of SFN on HMGB1-mediated septic responses and survival rate in a mouse sepsis model. The anti-inflammatory activities of SFN were monitored based on its effects on lipopolysaccharide (LPS)- or cecal ligation and puncture (CLP)-mediated release of HMGB1...
August 22, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28822504/is-smog-innocuous-air-pollution-and-cardiovascular-disease
#15
EDITORIAL
Sundeep Mishra
Air pollution is a significant environmental and health hazard. Earlier studies had examined the adverse health effects associated with short- and long-term exposure to particulate matter on respiratory disease. However, later studies demonstrated that was actually cardiovascular disease that accounted for majority of mortality. Furthermore, it was not gaseous pollutants like oxides of nitrate, sulfur, carbon mono-oxide or ozone but the particulate matter or PM, of fine or coarse size (PM2.5 and PM10) which was linearly associated with mortality; PM2...
July 2017: Indian Heart Journal
https://www.readbyqxmd.com/read/28816361/msel-1l-deficiency-affects-vasculogenesis-and-neural-stem-cell-lineage-commitment
#16
Marina Cardano, Giuseppe R Diaferia, Luciano Conti, Simona Baronchelli, Alessandro Sessa, Vania Broccoli, Andrea Barbieri, Pasquale De Blasio, Ida Biunno
mSEL-1L is a highly conserved ER-resident type I protein, involved in the degradation of misfolded peptides through the ubiquitin-proteasome system (UPS), a pathway known to control the plasticity of the vascular smooth muscle cells (VSMC) phenotype and survival. In this article, we demonstrate that mSEL-1L deficiency interferes with the murine embryonic vascular network, showing particular irregularities in the intracranic and intersomitic neurovascular units and in the cerebral capillary microcirculation...
August 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28805458/thermal-inactivation-of-listeria-monocytogenes-and-salmonella-during-water-and-steam-blanching-of-vegetables
#17
Erdogan Ceylan, Wendy McMahon, Donna M Garren
Thermal inactivation of Listeria monocytogenes and Salmonella was evaluated on peas, spinach, broccoli, potatoes, and carrots that were treated with hot water and steam. One gram-positive bacterium, L. monocytogenes, and one gram-negative bacterium, Salmonella, were selected as pertinent human pathogens for evaluation. Samples were inoculated with a composite of five strains each of L. monocytogenes and Salmonella to achieve approximately 10(8) to 10(9) CFU/g. Inoculated samples were treated with hot water at 85 and 87...
September 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28805380/evaluating-the-antibacterial-properties-of-polyacetylene-and-glucosinolate-compounds-with-further-identification-of-their-presence-within-various-carrot-daucus-carota-and-broccoli-brassica-oleracea-cultivars-using-high-performance-liquid-chromatography-with
#18
L Hinds, O Kenny, M B Hossain, D Walsh, E Sheehy, P Evans, M Gaffney, D K Rai
Ongoing consumer concerns over using synthetic additives in foods has strongly influenced efforts worldwide to source suitable natural alternatives. In this study, the antibacterial efficacy of polyacetylene and glucosinolate compounds was evaluated against both Gram positive and Gram negative bacterial strains. Falcarinol [minimum inhibitory concentration (MIC) = 18.8-37.6 μg/mL] demonstrated the best overall antibacterial activity, while sinigrin (MIC = 46.9-62.5 μg/mL) was the most active glucosinolate compound...
August 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28800824/assessing-the-antimicrobial-potential-of-aerosolised-electrochemically-activated-solutions-ecas-for-reducing-the-microbial-bio-burden-on-fresh-food-produce-held-under-cooled-or-cold-storage-conditions
#19
R M S Thorn, J Pendred, D M Reynolds
The main aim of this study was to assess the antimicrobial efficacy of electrochemically activated fog (ECAF) for reducing the microbial bio-burden on artificially inoculated fresh produce held under cooled (cucumber and vine tomatoes) or cold (rocket and broccoli) storage conditions. The ECAF treatment (1100 ± 5 mV ORP; 50 ± 5 mg L(-1) free chlorine; 2.7 ± 0.1 pH) resulted in a significant log reduction in the potential pathogen E. coli recovered from rocket (2.644 Log10 CFU g(-1)), broccoli (4...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28796173/effects-of-vegetables-on-cardiovascular-diseases-and-related-mechanisms
#20
REVIEW
Guo-Yi Tang, Xiao Meng, Ya Li, Cai-Ning Zhao, Qing Liu, Hua-Bin Li
Epidemiological studies have shown that vegetable consumption is inversely related to the risk of cardiovascular diseases. Moreover, research has indicated that many vegetables like potatoes, soybeans, sesame, tomatoes, dioscorea, onions, celery, broccoli, lettuce and asparagus showed great potential in preventing and treating cardiovascular diseases, and vitamins, essential elements, dietary fibers, botanic proteins and phytochemicals were bioactive components. The cardioprotective effects of vegetables might involve antioxidation; anti-inflammation; anti-platelet; regulating blood pressure, blood glucose, and lipid profile; attenuating myocardial damage; and modulating relevant enzyme activities, gene expression, and signaling pathways as well as some other biomarkers associated to cardiovascular diseases...
August 10, 2017: Nutrients
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