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https://www.readbyqxmd.com/read/28063994/heparin-prevents-zika-virus-induced-cytopathic-effects-in-human-neural-progenitor-cells
#1
Silvia Ghezzi, Lynsay Cooper, Alicia Rubio, Isabel Pagani, Maria Rosaria Capobianchi, Giuseppe Ippolito, Julien Pelletier, Maria Cecilia Z Meneghetti, Marcelo A Lima, Mark A Skidmore, Vania Broccoli, Edwin A Yates, Elisa Vicenzi
The recent Zika virus (ZIKV) outbreak, which mainly affected Brazil and neighbouring states, demonstrated the paucity of information concerning the epidemiology of several flaviruses, but also highlighted the lack of available agents with which to treat such emerging diseases. Here, we show that heparin, a widely used anticoagulant, while exerting a modest inhibitory effect on Zika Virus replication, fully prevents virus-induced cell death of human neural progenitor cells (NPCs).
January 5, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28054986/d-l-sulforaphane-induces-ros-dependent-apoptosis-in-human-gliomablastoma-cells-by-inactivating-stat3-signaling-pathway
#2
Ziwei Miao, Fei Yu, Yahao Ren, Jun Yang
d,l-Sulforaphane (SFN), a synthetic analogue of broccoli-derived isomer l-SFN, exerts cytotoxic effects on multiple tumor cell types through different mechanisms and is more potent than the l-isomer at inhibiting cancer growth. However, the means by which SFN impairs glioblastoma (GBM) cells remains poorly understood. In this study, we investigated the anti-cancer effect of SFN in GBM cells and determined the underlying molecular mechanisms. Cell viability assays, flow cytometry, immunofluorescence, and Western blot results revealed that SFN could induced apoptosis of GBM cells in a dose- and time-dependent manner, via up-regulation of caspase-3 and Bax, and down-regulation of Bcl-2...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28035167/optimization-of-an-incubation-step-to-maximize-sulforaphane-content-in-pre-processed-broccoli
#3
Andrea Mahn, Carmen Pérez
Sulforaphane is a powerful anticancer compound, found naturally in food, which comes from the hydrolysis of glucoraphanin, the main glucosinolate of broccoli. The aim of this work was to maximize sulforaphane content in broccoli by designing an incubation step after subjecting broccoli pieces to an optimized blanching step. Incubation was optimized through a Box-Behnken design using ascorbic acid concentration, incubation temperature and incubation time as factors. The optimal incubation conditions were 38 °C for 3 h and 0...
November 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28026010/temperature-and-light-conditions-at-different-latitudes-affect-sensory-quality-of-broccoli-florets-brassica-oleracea-l-var-italica
#4
Tor J Johansen, Jørgen Ab Mølmann, Gunnar B Bengtsson, Monica Schreiner, Pablo Velasco, Anne L Hykkerud, Elena Cartea, Per Lea, Josefine Skaret, Randi Seljåsen
BACKGROUND: Broccoli (Brassica oleracea L. var. italica) is a popular vegetable grown at a wide range of latitudes. Plants were grown in 2009-2011 in pots with standardized soil, irrigation and nutrient supply at natural temperature and light conditions at four locations (42-70 °N). A descriptive sensory analysis of broccoli florets was performed by a trained panel to examine any differences along the latitudinal gradient for 30 attributes within appearance, odour, taste/flavour and texture...
December 27, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28011683/larval-performance-and-adult-attraction-of-delia-platura-diptera-anthomyiidae-in-a-native-and-an-introduced-crop
#5
Patricia C Guerra, Clifford B Keil, Philip C Stevenson, Diego Mina, Servio Samaniego, Eduardo Peralta, Nelson Mazon, Timothy C B Chancellor
Delia platura Meigen is an important pest in crops around the world. Its host range includes almost 50 species, and it can develop in soil organic matter. In Ecuador, D. platura is a serious problem for the crop, Lupinus mutabilis Sweet (Chocho), and it also attacks broccoli (Brassica oleracea L.). After broccoli is harvested, crop residue is mixed with soil or collected and stored close to Chocho fields. The objectives of this study were to determine the adaptive responses of larvae reared on different hosts and whether D...
December 23, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27995045/in-vitro-interaction-of-metarhizium-anisopliae-ma9236-and-beauveria-bassiana-bb9205-with-heterorhabditis-bacteriophora-hni0100-for-the-control-of-plutella-xylostella
#6
J P Correa-Cuadros, A Sáenz-Aponte, M X Rodríguez-Bocanegra
The diamondback moth (Plutella xylostella) is a major pest of broccoli crops in Colombia. To control P. xylostella, we evaluated the interaction of Beauveria bassiana Bb9205 and Metarhizium anisopliae Ma9236 with Heterorhabditis bacteriophora HNI0100 and its bacterial symbiont Photorhabdus luminescens HNI0100. We used antagonism and disk diffusion assays with fungal extracts to test the interaction between symbiotic bacterium and fungi. P. luminescens inhibited the growth of B. bassiana and M. anisopliae up to 40% by the secretion of secondary metabolites, whereas fungal extracts did not inhibit P...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27992213/proposed-method-for-estimating-health-promoting-glucosinolates-and-hydrolysis-products-in-broccoli-brassica-oleracea-var-italica-using-relative-transcript-abundance
#7
Talon M Becker, Elizabeth H Jeffery, John A Juvik
Due to the importance of glucosinolates and their hydrolysis products in human nutrition and plant defense, optimizing the content of these compounds is a frequent breeding objective for Brassica crops. Toward this goal, we investigated the feasibility of using models built from relative transcript abundance data for the prediction of glucosinolate and hydrolysis product concentrations in broccoli. We report that predictive models explaining at least 50% of the variation for a number of glucosinolates and their hydrolysis products can be built for prediction within the same season, but prediction accuracy decreased when using models built from one season's data for prediction of an opposing season...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27989117/hplc-separation-of-sulforaphane-enantiomers-in-broccoli-and-its-sprouts-by-transformation-into-diastereoisomers-using-derivatization-with-s-leucine
#8
Makiko Okada, Atsushi Yamamoto, Sen-Ichi Aizawa, Atsushi Taga, Hiroyuki Terashima, Shuji Kodama
Racemic sulforaphane, which was derivatized with (S)-leucine (l-leucine), was resolved by reversed phase HPLC with UV detection. The optimum mobile phase conditions were found to be 10 mM citric acid (pH 2.8) containing 22% methanol at 35 °C using detection at 254 nm. Sulforaphane enantiomers in florets and stems of five brands of broccoli and leaves and stems of three brands of broccoli sprouts were analyzed by the proposed HPLC method. Both sulforaphane enantiomers were detected in all of the samples. The S/R ratios of sulforaphane in broccoli samples were 1...
December 27, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27979663/sulforaphane-inhibits-osteoclast-differentiation-by-suppressing-the-cell-cell-fusion-molecules-dc-stamp-and-oc-stamp
#9
Tomohiro Takagi, Hirofumi Inoue, Nobuyuki Takahashi, Rie Katsumata-Tsuboi, Mariko Uehara
Sulforaphane (SFN), a kind of isothiocyanate, is derived from broccoli sprouts. It has anti-tumor, anti-inflammatory, and anti-oxidation activity. The molecular function of SFN in the inhibition of osteoclast differentiation is not well-documented. In this study, we assessed the effect of SFN on osteoclast differentiation in vitro. SFN inhibited osteoclast differentiation in both bone marrow cells and RAW264.7 cells. Key molecules involved in the inhibitory effects of SFN on osteoclast differentiation were determined using a microarray analysis, which showed that SFN inhibits osteoclast-associated genes, such as osteoclast-associated receptor (OSCAR), nuclear factor of activated T cells cytoplasmic-1, tartrate-resistant acid phosphatase, and cathepsin K...
December 12, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27979631/indole-3-carbinol-i3c-analogues-are-potent-small-molecule-inhibitors-of-nedd4-1-ubiquitin-ligase-activity-that-disrupt-proliferation-of-human-melanoma-cells
#10
Jeanne G Quirit, Sergey N Lavrenov, Kevin Poindexter, Janice Xu, Christine Kyauk, Kathleen A Durkin, Ida Aronchik, Thomas Tomasiak, Yaroslav A Solomatin, Maria N Preobrazhenskaya, Gary L Firestone
The HECT domain-containing E3 ubiquitin ligase NEDD4-1 (Neural precursor cell Expressed Developmentally Down regulated gene 4-1) is frequently overexpressed in human cancers and displays oncogenic-like properties through the ubiquitin-dependent regulation of multiple protein substrates. However, little is known about small molecule enzymatic inhibitors of HECT domain-containing ubiquitin ligases. We now demonstrate that indole-3-carbinol (I3C), a natural anti-cancer phytochemical derived from cruciferous vegetables such as cabbage and broccoli, represents a new chemical scaffold of small molecule enzymatic inhibitors of NEDD4-1...
December 12, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27966726/broccoli-sprouts-in-analgesia-preclinical-in-vivo-studies
#11
Nieves Baenas, María Eva González-Trujano, Omar Guadarrama-Enríquez, Francisco Pellicer, Cristina García-Viguera, Diego A Moreno
BACKGROUND: Broccoli is a rich source of health-promoting glucosinolates, phenolic compounds, minerals and vitamins, which might have potential to alleviate pain. AIM: To explore the antinociceptive effects of a broccoli sprout aqueous extract (BSE) in experimental models of pain and an opioid mechanism. MATERIALS AND METHODS: the BSE was administered to mice and rats that were subjected to the writhing and formalin tests, respectively. Gastric damage or sedative-like response, as adverse effects observed in anti-inflammatory non-steroidal and opioid analgesic drugs, respectively, were also explored...
December 14, 2016: Food & Function
https://www.readbyqxmd.com/read/27942203/pharmacoutilization-of-epoetins-in-na%C3%A3-ve-patients-with-hematological-malignancies-in-an-unselected-italian-population-under-clinical-practice-setting-a-comparative-analysis-between-originator-and-biosimilars
#12
Valentina Perrone, Stefania Saragoni, Stefano Buda, Alessandro Broccoli, Luca Degli Esposti
AIM: The purpose of this study was to assess the prescription of epoetins and consumption of health care resources (in terms of drug treatments) in naïve patients with hematological malignancies in a real-world setting; in particular, we compared the results between reference product and biosimilar products. METHODS: An observational retrospective study based on administrative and laboratory databases of three local health units was conducted. All adults diagnosed with hematological malignancies and who had received at least one epoetin (either reference product or biosimilars) prescription for the first time between 1 January 2010 and 30 April 2012 (enrollment period) were included...
2016: Biologics: Targets & Therapy
https://www.readbyqxmd.com/read/27935686/neuroprotection-of-brain-cells-by-lipoic-acid-treatment-after-cellular-stress
#13
Sara Paradells-Navarro, María Soledad Benlloch-Navarro, María Inmaculada Almansa Frias, Ma Angeles Garcia-Esparza, Vania Broccoli, María Miranda, José Miguel Soria
We have previously observed that in vivo lipoic acid (LA) treatment induced a protective effect onto primary cortical neurons after brain injury. In an effort to better understand LA action mechanism in the brain, in the present study, we stressed brain cells in vitro and ex vivo and then analyzed by inmmunocytochemistry and biochemical assays, the changes induced by LA on cell survival and on the concentration of oxidative stress markers, such as glutathione (GSH), oxidized glutathione (GSSG), and malondialdehyde (MDA)...
December 9, 2016: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/27935214/stabilized-sulforaphane-for-clinical-use-phytochemical-delivery-efficiency
#14
Jed W Fahey, Kristina L Wade, Scott L Wehage, W David Holtzclaw, Hua Liu, Paul Talalay, Edward Fuchs, Katherine K Stephenson
SCOPE: The isothiocyanate sulforaphane (SF) from broccoli, is one of the most potent known inducers of the cytoprotective phase 2 response. Its role in a host of biochemical pathways make it a major component of plant-based protective strategies for enhancing healthspan. Many nutritional supplements are now marketed that purport to contain SF, which in plants exists as a stable precursor, a thioglucoside hydroxysulfate. However, SF in pure form must be stabilized for use in supplements...
December 9, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27905406/differential-roles-for-l-type-calcium-channel-subtypes-in-alcohol-dependence
#15
Stefanie Uhrig, David Vandael, Andrea Marcantoni, Nina Dedic, Ainhoa Bilbao, Miriam A Vogt, Natalie Hirth, Laura Broccoli, Rick E Bernardi, Kai Schönig, Peter Gass, Dusan Bartsch, Rainer Spanagel, Jan M Deussing, Wolfgang H Sommer, Emilio Carbone, Anita C Hansson
It has previously been shown that the inhibition of L-type calcium channels (LTCCs) decreases alcohol consumption, although the contribution of the central LTCC subtypes Cav1.2 and Cav1.3 remains unknown. Here, we determined changes in Cav1.2 (Cacna1c) and Cav1.3 (Cacna1d) mRNA and protein expression in alcohol dependent rats during protracted abstinence and naïve controls using in situ hybridization and Western Blot analysis. Functional validation was obtained by electrophysiological recordings of calcium currents in dissociated hippocampal pyramidal neurons...
December 1, 2016: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/27900399/quantitative-trait-loci-mapping-of-heat-tolerance-in-broccoli-brassica-oleracea-var-italica-using-genotyping-by-sequencing
#16
Sandra E Branham, Zachary J Stansell, David M Couillard, Mark W Farnham
Five quantitative trait loci and one epistatic interaction were associated with heat tolerance in a doubled haploid population of broccoli evaluated in three summer field trials. Predicted rising global temperatures due to climate change have generated a demand for crops that are resistant to yield and quality losses from heat stress. Broccoli (Brassica oleracea var. italica) is a cool weather crop with high temperatures during production decreasing both head quality and yield. Breeding for heat tolerance in broccoli has potential to both expand viable production areas and extend the growing season but breeding efficiency is constrained by limited genetic information...
November 29, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27889290/randomized-split-body-single-blinded-clinical-trial-of-topical-broccoli-sprout-extract-assessing-the-feasibility-of-its-use-in-keratin-based-disorders
#17
Michelle L Kerns, Lark Guss, Jed Fahey, Bernard Cohen, Jill M C Hakim, Sarah Sung, Rosemary G Lu, Pierre A Coulombe
BACKGROUND: Epidermolysis bullosa simplex is a skin-blistering disorder caused by mutations in keratin (K)14 or K5. Treatment with nuclear factor (erythroid-derived 2)-like 2 inducer sulforaphane ameliorated skin blistering in Krt14-null mice, correlating with induction of K17. To be therapeutically useful for epidermolysis bullosa simplex, topical broccoli sprout extract (BSE), enriched for sulforaphane, would ideally induce the expression of homologous keratins (eg, K6, K17, K16) in the basal layer of human epidermis without impacting expression of defective keratins (K5/K14)...
November 23, 2016: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/27888880/optimizing-outcomes-in-primary-mediastinal-b-cell-lymphoma
#18
REVIEW
Pier Luigi Zinzani, Alessandro Broccoli
Primary mediastinal B-cell lymphoma is characterized by a high chance of cure, and cured patients have a long disease-free life-expectancy; however, prognosis is severe in the case of relapsed or refractory disease. The initial use of the most effective chemoimmunotherapy regimen is therefore crucial. Understanding who will benefit from postinduction radiotherapy is also of paramount importance; positron emission tomography may be a reliable guide for physicians in determining which patients will require consolidation...
December 2016: Hematology/oncology Clinics of North America
https://www.readbyqxmd.com/read/27885677/chemical-composition-of-microalgae-heterochlorella-luteoviridis-and-dunaliella-tertiolecta-with-emphasis-on-carotenoids
#19
Andressa Bacalau Diprat, Tania Menegol, Juliana Ferreira Boelter, Ariane Zmozinski, Maria Goreti Rodrigues Vale, Eliseu Rodrigues, Rosane Rech
BACKGROUND: Microalgae have been used as food supplements due their high protein, polyunsaturated fatty acids and carotenoid contents. As different carotenoids have distinct properties, and carotenoid composition from microalgae were poorly explored in the literature, this study determined the complete carotenoid composition from two microalgae species, Heterochlorella luteoviridis and Dunaliella tertiolecta, using high-performance liquid chromatography coupled to diode array detector and a mass spectrometry (HPLC-DAD-MS(2) )...
November 24, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27883018/cytotoxic-and-genotoxic-potential-of-food-borne-nitriles-in-a-liver-in-vitro-model
#20
Franziska Kupke, Corinna Herz, Franziska S Hanschen, Stefanie Platz, Grace A Odongo, Simone Helmig, María M Bartolomé Rodríguez, Monika Schreiner, Sascha Rohn, Evelyn Lamy
Isothiocyanates are the most intensively studied breakdown products of glucosinolates from Brassica plants and well recognized for their pleiotropic effects against cancer but also for their genotoxic potential. However, knowledge about the bioactivity of glucosinolate-borne nitriles in foods is very poor. As determined by GC-MS, broccoli glucosinolates mainly degrade to nitriles as breakdown products. The cytotoxicity of nitriles in human HepG2 cells and primary murine hepatocytes was marginal as compared to isothiocyanates...
November 24, 2016: Scientific Reports
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