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Teresa Oliviero, Simone Lamers, Edoardo Capuano, Matthijs Dekker, Ruud Verkerk
SCOPE: Optimization of bioavailability of dietary bioactive health-beneficial compounds is as important as increasing their concentration in foods. The aim of this study was to explore the change in bioavailability of isothiocyanates in broccoli sprouts incorporated in protein, fibre and lipid gels. METHODS AND RESULTS: Five participants took part in a cross-over study and collected timed urine samples before up to 24-hours after consumption of proteins, dietary fibres and lipids gels containing broccoli spouts powder...
March 12, 2018: Molecular Nutrition & Food Research
Morgan C Broccoli, Rachel Moresky, Julia Dixon, Ivy Muya, Cara Taubman, Lee A Wallis, Emilie J Calvello Hynes
Facility-based emergency care delivery in low-income and middle- income countries is expanding rapidly, particularly in Africa. Unfortunately, these efforts rarely include measurement of the quality or the impact of care provided, which is essential for improvement of care provision. Our aim was to determine context-appropriate quality indicators that will allow uniform and objective data collection to enhance emergency care delivery throughout Africa. We undertook a multiphase expert consensus process to identify, rank and refine quality indicators...
2018: BMJ Global Health
Hanna Bessler, Meir Djaldetti
The favourable properties of broccoli on human health are due to their abundant content of vitamins, minerals and isothiocyanates, the sulforaphane (SFN) being the most important. SFN is created from its precursor glucoraphanin and it is released by myrosinase enzymes produced during crushing of the plant. SFN has been shown to possess anti-inflammatory and anticancer properties. The aim of the study was to determine if SFN may affect the immune dialogue between human peripheral blood mononuclear cells (PBMCs) and HT-29 and RKO human colon cancer cells lines...
March 7, 2018: International Journal of Food Sciences and Nutrition
Kiran R Gadhave, Paul F Devlin, Andreas Ebertz, Arabella Ross, Alan C Gange
The use of microbial inoculants containing plant growth-promoting rhizobacteria as a promoter of plant fitness and health is becoming increasingly popular in agriculture. However, whether and how these bacteria affect indigenous bacterial communities in field conditions is sparsely explored. We studied the effects of seed inoculation and field soil application of ubiquitous soil bacteria, B. cereus, B. subtilis, and B. amyloliquefaciens, on the diversity, evenness, and richness of endophytic bacterial communities in sprouting broccoli roots using high-throughput metagenome sequencing...
March 6, 2018: Microbial Ecology
Vittorio Stefoni, Alessandro Broccoli, Lisa Argnani, Elena Sabattini, Michele Cavo, Pier Luigi Zinzani
No abstract text is available yet for this article.
February 16, 2018: Clinical Lymphoma, Myeloma & Leukemia
Takahiro Yamaguchi, Ryuji Kawahara, Chihiro Katsukawa, Masashi Kanki, Tetsuya Harada, Shinya Yonogi, Satomi Iwasaki, Hirokazu Uehara, Saori Okajima, Hiroshi Nishimura, Kazushi Motomura, Masaya Miyazono, Yuko Kumeda, Kentaro Kawatsu
In September 2016, 140 patients with primary symptoms of sore throat and fever were identified in a school dormitory in Osaka, Japan. Epidemiological and laboratory investigations determined that this symptomatic condition was a food-borne outbreak of group G Streptococcus (GGS), with GGS being isolated from samples from patients, cooks and foods. The strain of GGS was identified as Streptococcus dysgalactiae subsp. equisimilis (SDSE) of two emm types ( stG 652.0 and st C36.0). The causative food, a broccoli salad, was contaminated with the two types of SDSE, totaling 1...
February 28, 2018: Journal of Clinical Microbiology
Rocío Recio, Eleonora Elhalem, Juan M Benito, Inmaculada Fernández, Noureddine Khiar
Sulforaphane (SFN), a phytochemical isolated from broccoli, is an important antitumoral compound with additional beneficial effect on other important diseases. However, the chemical instability of SFN has hampered its clinical use. In order to circumvent this problem, we report the first comparative study on the inclusion complexes of SFN and SFN homologues with different cyclodextrins by NMR spectroscopy. From this study it has been shown that α-CD is the most indicated cyclodextrin for the stabilization of SFN and SFN homologues, and that the highest affinity constant is that of the isothiocyanate obtained from the wasabi...
May 1, 2018: Carbohydrate Polymers
Alessandro Pileri, Carlotta Baraldi, Alessandro Broccoli, Roberto Maglie, Annalisa Patrizi, Pierluigi Zinzani, Emiliano Antiga
No abstract text is available yet for this article.
February 26, 2018: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
Malin Nordenström, Anastasia V Riazanova, Mikael Järn, Thomas Paulraj, Charlotta Turner, Valter Ström, Richard T Olsson, Anna J Svagan
Microcapsules with specific functional properties, related to the capsule wall and core, are highly desired in a number of applications. In this study, hybrid cellulose microcapsules (1.2 ± 0.4 µm in diameter) were prepared by nanoengineering the outer walls of precursor capsules. Depending on the preparation route, capsules with different surface roughness (raspberry or broccoli-like), and thereby different wetting properties, could be obtained. The tunable surface roughness was achieved as a result of the chemical and structural properties of the outer wall of a precursor capsule, which combined with a new processing route allowed in-situ formation of silica nanoparticles (30-40 nm or 70 nm in diameter)...
February 26, 2018: Scientific Reports
Dushani L Palliyaguru, Jian-Min Yuan, Thomas W Kensler, Jed W Fahey
Isothiocyanates from cruciferous vegetables have been studied extensively in cells and in animals for their disease preventive and therapeutic effects. However, translating their utility to human populations has been both limited and challenging. Herein, clinical trials employing two isothiocyanates, sulforaphane (SFN; 1-Isothiocyanato-4-(methylsulfinyl) butane) and phenethyl isothiocyanate (PEITC; 2-isothiocyanatoethylbenzene) that are isolated principally from broccoli and watercress, respectively, are summarized and discussed...
February 21, 2018: Molecular Nutrition & Food Research
Angelo Iannielli, Simone Bido, Lucrezia Folladori, Alice Segnali, Cinzia Cancellieri, Alessandra Maresca, Luca Massimino, Alicia Rubio, Giuseppe Morabito, Leonardo Caporali, Francesca Tagliavini, Olimpia Musumeci, Giuliana Gregato, Erwan Bezard, Valerio Carelli, Valeria Tiranti, Vania Broccoli
Dysfunctions in mitochondrial dynamics and metabolism are common pathological processes associated with Parkinson's disease (PD). It was recently shown that an inherited form of PD and dementia is caused by mutations in the OPA1 gene, which encodes for a key player in mitochondrial fusion and structure. iPSC-derived neural cells from these patients exhibited severe mitochondrial fragmentation, respiration impairment, ATP deficits, and heightened oxidative stress. Reconstitution of normal levels of OPA1 in PD-derived neural cells normalized mitochondria morphology and function...
February 20, 2018: Cell Reports
Yoshio Makino, Yuto Nishimura, Seiichi Oshita, Takaharu Mizosoe, Takashi Akihiro
Sulforaphane is a phytochemical that is usually found in cruciferous vegetables and is known to have a depressive effect on gastric cancer. Preliminary investigations showed that the sulforaphane concentration in broccoli (Brassica oleracea var. italica) florets increased under anoxia. Therefore, in the present study, we examined the effect of different atmospheric conditions on the sulforaphane concentration in broccoli and also tested whether there are concurrent effects on the concentration of ethanol, which is an unfavorable byproduct of fermentation...
2018: PloS One
Carmen K Blubaugh, Jacob S Asplund, Sanford D Eigenbrode, Matthew J Morra, Christopher R Philips, Inna E Popova, John P Reganold, William E Snyder
Plant defenses often mediate whether competing chewing and sucking herbivores indirectly benefit or harm one another. Dual guild herbivory also can muddle plant signals used by specialist natural enemies to locate prey, further complicating the net impact of herbivore-herbivore interactions in naturally diverse settings. While dual guild herbivore communities are common in nature, their community trilevel consequences are unclear, as chemically mediated tri-trophic interactions are rarely evaluated in field environments...
February 21, 2018: Ecology
Vardine Sahakyan, Robin Duelen, Wai Long Tam, Scott J Roberts, Hanne Grosemans, Pieter Berckmans, Gabriele Ceccarelli, Gloria Pelizzo, Vania Broccoli, Jan Deprest, Frank P Luyten, Catherine M Verfaillie, Maurilio Sampaolesi
Neural tube defects (NTDs) are severe congenital abnormalities, caused by failed closure of neural tube during early embryonic development. Periconceptional folic acid (FA) supplementation greatly reduces the risk of NTDs. However, the molecular mechanisms behind NTDs and the preventive role of FA remain unclear. Here, we use human induced pluripotent stem cells (iPSCs) derived from fetuses with spina bifida aperta (SBA) to study the pathophysiology of NTDs and explore the effects of FA exposure. We report that FA exposure in SBA model is necessary for the proper formation and maturation of neural tube structures and robust differentiation of mesodermal derivatives...
February 13, 2018: Scientific Reports
Lizhen Li, Jianxiong Hao, Shuhui Song, Satoru Nirasawa, Zhengqiang Jiang, Haijie Liu
The producers of broccoli sprouts have become increasingly interested in improving their sulforaphane content. This study has evaluated the effects of slightly acidic electrolyzed water (SAEW) with different available chlorine concentrations (ACC) on broccoli sprouts: their content of some bioactive compounds such as glucosinolates, their morphology, and their total bacterial counts. The results have shown that SAEW might affect the content of sulforaphane by influencing the content of glucosinolates and the activity of myrosinase...
March 2018: Food Research International
Min-Soo Kim, Jin-Woo Bae, Eun-Jin Park
Fresh vegetables harbor diverse bacterial populations on their surfaces. However, information on this microbiota is limited to a few types of fresh vegetables, and little is known about how it varies with geography and host condition. Here, we analyzed bacterial communities on the floret surfaces of 66 field-grown broccolis collected from 22 farms in four farming regions of Jeju Island using 454 pyrosequencing of 16S rRNA amplicons, and determined their relationship to farming region and host-associated factors...
February 2, 2018: Applied and Environmental Microbiology
Maria Del Carmen Martínez-Ballesta, Pablo García-Gomez, Lucía Yepes-Molina, Angel L Guarnizo, José A Teruel, Micaela Carvajal
The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describe for first time an increase in plant aquaporins acetylation under high salinity. Higher osmotic water permeability in NaCl vesicles has been related to higher acetylation, upregulation of aquaporins, and a more stable environment to thermal denaturation...
2018: PloS One
Milad Pero, Gholamreza Askari, Torstein Skåra, Dagbjørn Skipnes, Hossein Kiani
BACKGROUND: Vacuum packed broccoli stems and florets were subjected to heat treatment (60 - 99 °C) for various time intervals. The activity of peroxidase was measured after processing. Thermally processed samples were then stored at 4 °C for 35 days and the color of the samples were measured every 7 days. Effects of parameters (heating temperature and duration, storage time) on the color of broccoli were modeled and simulated by artificial neural network. RESULTS: Simulations confirmed that stems were predicted to be more prone to changes than florets...
February 8, 2018: Journal of the Science of Food and Agriculture
Steven J Lehotay, Lijun Han, Yelena Sapozhnikova
In routine monitoring of foods, reduction of analyzed test portion size generally leads to higher sample throughput, less labor, and lower costs of monitoring, but to meet analytical needs, the test portions still need to accurately represent the original bulk samples. With the intent to determine minimal fit-for-purpose sample size, analyses were conducted for up to 93 incurred and added pesticide residues in 10 common fruits and vegetables processed using different sample comminution equipment. The commodities studied consisted of apple, banana, broccoli, celery, grape, green bean, peach, potato, orange, and squash...
February 6, 2018: Analytical and Bioanalytical Chemistry
Katherine M Phillips, Maria Teresa Tarrago-Trani, Ryan M McGinty, Amy S Rasor, David B Haytowitz, Pamela R Pehrsson
BACKGROUND: Seasonal variation of vitamin C in fresh fruits and vegetables is not reflected in food composition database average values, yet many factors influence content and retention. RESULTS: Fresh fruits and vegetables were sampled on three occasions in each season, from the same local retail outlets, for one or two years. Vitamin C was significantly higher in winter-sampled spinach (436 mg kg-1 ) compared to spring (298) and summer/fall (180); in potatoes in summer/fall (156) versus winter/spring (106); and oranges in winter (616), spring (592), and summer (506)...
February 6, 2018: Journal of the Science of Food and Agriculture
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