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https://www.readbyqxmd.com/read/28195505/comparative-study-of-elemental-nutrients-in-organic-and-conventional-vegetables-using-laser-induced-breakdown-spectroscopy-libs
#1
Chet R Bhatt, Bader Alfarraj, Charles T Ghany, Fang Y Yueh, Jagdish P Singh
In this study, the laser-induced breakdown spectroscopy (LIBS) technique was used to identify and compare the presence of major nutrient elements in organic and conventional vegetables. Different parts of cauliflowers and broccolis were used as working samples. Laser-induced breakdown spectra from these samples were acquired at optimum values of laser energy, gate delay, and gate width. Both univariate and multivariate analyses were performed for the comparison of these organic and conventional vegetable flowers...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28191778/generation-of-human-induced-pluripotent-stem-cell-derived-bona-fide-neural-stem-cells-for-ex-vivo-gene-therapy-of-metachromatic-leukodystrophy
#2
Vasco Meneghini, Giacomo Frati, Davide Sala, Silvia De Cicco, Marco Luciani, Chiara Cavazzin, Marianna Paulis, Wieslawa Mentzen, Francesco Morena, Serena Giannelli, Francesca Sanvito, Anna Villa, Alessandro Bulfone, Vania Broccoli, Sabata Martino, Angela Gritti
Allogeneic fetal-derived human neural stem cells (hfNSCs) that are under clinical evaluation for several neurodegenerative diseases display a favorable safety profile, but require immunosuppression upon transplantation in patients. Neural progenitors derived from patient-specific induced pluripotent stem cells (iPSCs) may be relevant for autologous ex vivo gene-therapy applications to treat genetic diseases with unmet medical need. In this scenario, obtaining iPSC-derived neural stem cells (NSCs) showing a reliable "NSC signature" is mandatory...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28181675/a-placebo-controlled-double-blinded-randomized-pilot-study-of-combination-phytotherapy-in-biochemically-recurrent-prostate-cancer
#3
M Diana van Die, Scott G Williams, Jon Emery, Kerry M Bone, Jeremy M G Taylor, Elizabeth Lusk, Marie V Pirotta
BACKGROUND: Men with biochemical recurrence of prostate cancer following local therapies often use natural supplements in an attempt to delay metastases and/or avoid the need for more aggressive treatments with undesirable side-effects. While there is a growing body of research into phytotherapeutic agents in this cohort, with some promising results, as yet no definitive recommendations can be made. This pilot study was undertaken to assess the feasibility of a fully-powered study to examine the effects of this phytotherapeutic intervention (containing turmeric, resveratrol, green tea and broccoli sprouts) on PSA doubling time in men with biochemical recurrence with a moderate PSA rise rate...
February 9, 2017: Prostate
https://www.readbyqxmd.com/read/28180326/use-of-baby-spinach-and-broccoli-for-imaging-of-structured-cellular-rnas
#4
Maho Okuda, Dominique Fourmy, Satoko Yoshizawa
No abstract text is available yet for this article.
February 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28176666/role-of-sulforaphane-in-protection-of-gastrointestinal-tract-against-h-pylori-and-nsaid-induced-oxidative-stress
#5
Akinori Yanaka
BACKGROUND: Sulforaphane (SFN), a phytochemical found in abundance in broccoli sprouts, potently induces a variety of antioxidant enzymes, and thereby protects cells from injury induced by various kinds of oxidative stresses. It has been suggested that both H. pylori infection and intake of non-steroidal anti-inflammatory drugs (NSAIDs) induce chronic oxidative stress in gastrointestinal (GI) mucosa, thereby causing mucosal injury in the GI tract. Therefore, it would be a reasonable assumption that SFN protects GI mucosa against oxidative injury induced by H...
February 6, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28176652/mechanisms-for-inhibition-of-colon-cancer-cells-by-sulforaphane-through-epigenetic-modulation-of-microrna-21-and-human-telomerase-reverse-transcriptase-htert-down-regulation
#6
Samantha L Martin, Rishabh Kala, Trygve O Tollefsbol
Epigenetic modulations such as histone modifications are becoming increasingly valued for their ability to modify genes without altering the DNA sequence. Many bioactive compounds have been shown to alter genetic and epigenetic profiles in various forms of 6 cancers. Of the many dietary phytochemicals, sulforaphane (SFN), found in cruciferous vegetables such as kale, cabbage and broccoli sprouts, has been present as one of the most potent (histone deacetylase) HDAC inhibitors to date. Recently, it has been 9 identified that HDAC inhibitors may play a vital role in regulating microRNAs (miRNAs) in many human cancers...
February 5, 2017: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/28169300/two-factor-based-reprogramming-of-rodent-and-human-fibroblasts-into-schwann-cells
#7
Pietro Giuseppe Mazzara, Luca Massimino, Marta Pellegatta, Giulia Ronchi, Alessandra Ricca, Angelo Iannielli, Serena Gea Giannelli, Marco Cursi, Cinzia Cancellieri, Alessandro Sessa, Ubaldo Del Carro, Angelo Quattrini, Stefano Geuna, Angela Gritti, Carla Taveggia, Vania Broccoli
Schwann cells (SCs) generate the myelin wrapping of peripheral nerve axons and are promising candidates for cell therapy. However, to date a renewable source of SCs is lacking. In this study, we show the conversion of skin fibroblasts into induced Schwann cells (iSCs) by driving the expression of two transcription factors, Sox10 and Egr2. iSCs resembled primary SCs in global gene expression profiling and PNS identity. In vitro, iSCs wrapped axons generating compact myelin sheaths with regular nodal structures...
February 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28169290/knockdown-of-a-cellulose-synthase-gene-boicesa-affects-the-leaf-anatomy-cellulose-content-and-salt-tolerance-in-broccoli
#8
Shuangtao Li, Lei Zhang, Ying Wang, Fengfeng Xu, Mengyun Liu, Peng Lin, Shuxin Ren, Rui Ma, Yang-Dong Guo
Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the CesA gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28159293/determination-of-pesticide-residues-in-leafy-vegetables-at-parts-per-billion-levels-by-a-chemometric-study-using-gc-ecd-in-cameron-highlands-malaysia
#9
Yang Farina, Md Pauzi Abdullah, Nusrat Bibi, Wan Mohd Afiq Wan Mohd Khalik
A simple and sensitive analytical method has been developed employing gas chromatography coupled with electron capture detector (GC-ECD), and validated for screening and quantification of 15 pesticide residues at trace levels in cabbage, broccoli, cauliflower, lettuce, celery, spinach, and mustard. The method consists of two steps, first, to determine the significance of each factor by Pareto chart followed by optimization of these significant factors using central composite design (CCD). Minitab statistical software was used for these multivariate experiments for the generation of 2(4-1) design and CCD matrices...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28145738/influence-of-a-school-based-cooking-course-on-students-food-preferences-cooking-skills-and-confidence
#10
Rola Zahr, Lindiwe Sibeko
A quasi-experimental study was conducted to evaluate the influence of Project CHEF, a hands-on cooking and tasting program offered in Vancouver public schools, on students' food preferences, cooking skills, and confidence. Grade 4 and 5 students in an intervention group (n = 68) and a comparison group (n = 32) completed a survey at baseline and 2 to 3 weeks later. Students who participated in Project CHEF reported an increased familiarity and preference for the foods introduced through the program. This was statistically significant (P ≤ 0...
February 1, 2017: Canadian Journal of Dietetic Practice and Research
https://www.readbyqxmd.com/read/28131897/long-noncoding-rnas-and-sulforaphane-a-target-for-chemoprevention-and-suppression-of-prostate-cancer
#11
Laura M Beaver, Rachael Kuintzle, Alex Buchanan, Michelle W Wiley, Sarah T Glasser, Carmen P Wong, Gavin S Johnson, Jeff H Chang, Christiane V Löhr, David E Williams, Roderick H Dashwood, David A Hendrix, Emily Ho
Long noncoding RNAs (lncRNAs) have emerged as important in cancer development and progression. The impact of diet on lncRNA expression is largely unknown. Sulforaphane (SFN), obtained from vegetables like broccoli, can prevent and suppress cancer formation. Here we tested the hypothesis that SFN attenuates the expression of cancer-associated lncRNAs. We analyzed whole-genome RNA-sequencing data of normal human prostate epithelial cells and prostate cancer cells treated with 15 μM SFN or dimethylsulfoxide. SFN significantly altered expression of ~100 lncRNAs in each cell type and normalized the expression of some lncRNAs that were differentially expressed in cancer cells...
January 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28130586/gene-therapy-approaches-in-the-non-human-primate-model-of-parkinson-s-disease
#12
REVIEW
D Pignataro, D Sucunza, A J Rico, I G Dopeso-Reyes, E Roda, A I Rodríguez-Perez, J L Labandeira-Garcia, V Broccoli, S Kato, K Kobayashi, José L Lanciego
The field of gene therapy has recently witnessed a number of major conceptual changes. Besides the traditional thinking that comprises the use of viral vectors for the delivery of a given therapeutic gene, a number of original approaches have been recently envisaged, focused on using vectors carrying genes to further modify basal ganglia circuits of interest. It is expected that these approaches will ultimately induce a therapeutic potential being sustained by gene-induced changes in brain circuits. Among others, at present, it is technically feasible to use viral vectors to (1) achieve a controlled release of neurotrophic factors, (2) conduct either a transient or permanent silencing of any given basal ganglia circuit of interest, (3) perform an in vivo cellular reprogramming by promoting the conversion of resident cells into dopaminergic-like neurons, and (4) improving levodopa efficacy over time by targeting aromatic L-amino acid decarboxylase...
January 27, 2017: Journal of Neural Transmission
https://www.readbyqxmd.com/read/28115372/peripheral-t-cell-lymphoma-not-otherwise-specified
#13
Alessandro Broccoli, Pier Luigi Zinzani
Peripheral T-cell lymphoma, not otherwise specified, is a broad category of biologically and clinically heterogeneous diseases, which can not be further classified into any other of the existing entities defined by the World Health Organization classification. Anthracycline-containing regimens, namely cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP), nowadays represent the standard first-line treatment; for patients who achieve a satisfactory response, a consolidation by means of autologous stem cell transplantation may offer a greater chance of long-term survival...
January 23, 2017: Blood
https://www.readbyqxmd.com/read/28063994/heparin-prevents-zika-virus-induced-cytopathic-effects-in-human-neural-progenitor-cells
#14
Silvia Ghezzi, Lynsay Cooper, Alicia Rubio, Isabel Pagani, Maria Rosaria Capobianchi, Giuseppe Ippolito, Julien Pelletier, Maria Cecilia Z Meneghetti, Marcelo A Lima, Mark A Skidmore, Vania Broccoli, Edwin A Yates, Elisa Vicenzi
The recent Zika virus (ZIKV) outbreak, which mainly affected Brazil and neighbouring states, demonstrated the paucity of information concerning the epidemiology of several flaviruses, but also highlighted the lack of available agents with which to treat such emerging diseases. Here, we show that heparin, a widely used anticoagulant, while exerting a modest inhibitory effect on Zika Virus replication, fully prevents virus-induced cell death of human neural progenitor cells (NPCs).
January 5, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28054986/d-l-sulforaphane-induces-ros-dependent-apoptosis-in-human-gliomablastoma-cells-by-inactivating-stat3-signaling-pathway
#15
Ziwei Miao, Fei Yu, Yahao Ren, Jun Yang
d,l-Sulforaphane (SFN), a synthetic analogue of broccoli-derived isomer l-SFN, exerts cytotoxic effects on multiple tumor cell types through different mechanisms and is more potent than the l-isomer at inhibiting cancer growth. However, the means by which SFN impairs glioblastoma (GBM) cells remains poorly understood. In this study, we investigated the anti-cancer effect of SFN in GBM cells and determined the underlying molecular mechanisms. Cell viability assays, flow cytometry, immunofluorescence, and Western blot results revealed that SFN could induced apoptosis of GBM cells in a dose- and time-dependent manner, via up-regulation of caspase-3 and Bax, and down-regulation of Bcl-2...
January 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28035167/optimization-of-an-incubation-step-to-maximize-sulforaphane-content-in-pre-processed-broccoli
#16
Andrea Mahn, Carmen Pérez
Sulforaphane is a powerful anticancer compound, found naturally in food, which comes from the hydrolysis of glucoraphanin, the main glucosinolate of broccoli. The aim of this work was to maximize sulforaphane content in broccoli by designing an incubation step after subjecting broccoli pieces to an optimized blanching step. Incubation was optimized through a Box-Behnken design using ascorbic acid concentration, incubation temperature and incubation time as factors. The optimal incubation conditions were 38 °C for 3 h and 0...
November 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28026010/temperature-and-light-conditions-at-different-latitudes-affect-sensory-quality-of-broccoli-florets-brassica-oleracea-l-var-italica
#17
Tor J Johansen, Jørgen Ab Mølmann, Gunnar B Bengtsson, Monica Schreiner, Pablo Velasco, Anne L Hykkerud, Elena Cartea, Per Lea, Josefine Skaret, Randi Seljåsen
BACKGROUND: Broccoli (Brassica oleracea L. var. italica) is a popular vegetable grown at a wide range of latitudes. Plants were grown in 2009-2011 in pots with standardized soil, irrigation and nutrient supply at natural temperature and light conditions at four locations (42-70 °N). A descriptive sensory analysis of broccoli florets was performed by a trained panel to examine any differences along the latitudinal gradient for 30 attributes within appearance, odour, taste/flavour and texture...
December 27, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28011683/larval-performance-and-adult-attraction-of-delia-platura-diptera-anthomyiidae-in-a-native-and-an-introduced-crop
#18
Patricia C Guerra, Clifford B Keil, Philip C Stevenson, Diego Mina, Servio Samaniego, Eduardo Peralta, Nelson Mazon, Timothy C B Chancellor
Delia platura Meigen is an important pest in crops around the world. Its host range includes almost 50 species, and it can develop in soil organic matter. In Ecuador, D. platura is a serious problem for the crop, Lupinus mutabilis Sweet (Chocho), and it also attacks broccoli (Brassica oleracea L.). After broccoli is harvested, crop residue is mixed with soil or collected and stored close to Chocho fields. The objectives of this study were to determine the adaptive responses of larvae reared on different hosts and whether D...
December 23, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27995045/in-vitro-interaction-of-metarhizium-anisopliae-ma9236-and-beauveria-bassiana-bb9205-with-heterorhabditis-bacteriophora-hni0100-for-the-control-of-plutella-xylostella
#19
J P Correa-Cuadros, A Sáenz-Aponte, M X Rodríguez-Bocanegra
The diamondback moth (Plutella xylostella) is a major pest of broccoli crops in Colombia. To control P. xylostella, we evaluated the interaction of Beauveria bassiana Bb9205 and Metarhizium anisopliae Ma9236 with Heterorhabditis bacteriophora HNI0100 and its bacterial symbiont Photorhabdus luminescens HNI0100. We used antagonism and disk diffusion assays with fungal extracts to test the interaction between symbiotic bacterium and fungi. P. luminescens inhibited the growth of B. bassiana and M. anisopliae up to 40% by the secretion of secondary metabolites, whereas fungal extracts did not inhibit P...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27992213/proposed-method-for-estimating-health-promoting-glucosinolates-and-hydrolysis-products-in-broccoli-brassica-oleracea-var-italica-using-relative-transcript-abundance
#20
Talon M Becker, Elizabeth H Jeffery, John A Juvik
Due to the importance of glucosinolates and their hydrolysis products in human nutrition and plant defense, optimizing the content of these compounds is a frequent breeding objective for Brassica crops. Toward this goal, we investigated the feasibility of using models built from relative transcript abundance data for the prediction of glucosinolate and hydrolysis product concentrations in broccoli. We report that predictive models explaining at least 50% of the variation for a number of glucosinolates and their hydrolysis products can be built for prediction within the same season, but prediction accuracy decreased when using models built from one season's data for prediction of an opposing season...
January 18, 2017: Journal of Agricultural and Food Chemistry
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