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https://www.readbyqxmd.com/read/28544646/evaluating-the-accuracy-and-economic-value-of-a-new-test-in-the-absence-of-a-perfect-reference-test
#1
Xuanqian Xie, Alison Sinclair, Nandini Dendukuri
BACKGROUND: Streptococcus pneumoniae (SP) pneumonia is often treated empirically as diagnosis is challenging because of the lack of a perfect test. Using BinaxNOW-SP, a urinary antigen test, as an add-on to standard cultures may not only increase diagnostic yield but also increase costs. OBJECTIVE: To estimate the sensitivity and specificity of BinaxNOW-SP and subsequently estimate the cost-effectiveness of adding BinaxNOW-SP to the diagnostic work-up. DESIGN: We fit a Bayesian latent-class meta-analysis model to obtain estimates of BinaxNOW-SP accuracy that adjust for the imperfect accuracy of culture...
May 23, 2017: Research Synthesis Methods
https://www.readbyqxmd.com/read/28543962/effects-of-meiotic-inhibitors-and-gonadotrophins-on-porcine-oocytes-in-vitro-maturation-fertilization-and-development
#2
M A Gil, A Nohalez, C A Martinez, J R Ake-Villanueva, F Centurion-Castro, C Maside, C Cuello, J Roca, I Parrilla, E A Martinez
This study evaluated the effect of three reversible meiotic inhibitors (MINs) and their interaction with gonadotrophins (Gns) on the meiotic maturation and developmental competence of porcine oocytes. In experiment 1, the oocytes were matured for 22 hr in the presence or absence of dbcAMP (1 mM), cycloheximide (7 μM) or cilostamide (20 μM) with or without Gns, and for an additional 22 hr in the absence of MINs and Gns. At 22 hr of maturation, regardless of the presence of Gns, a higher proportion (p < ...
May 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28542923/the-polysialic-acid-mimetics-idarubicin-and-irinotecan-stimulate-neuronal-survival-and-neurite-outgrowth-and-signal-via-protein-kinase-c
#3
Gabriele Loers, Steven Astafiev, Yuliya Hapiak, Vedangana Saini, Bibhudatta Mishra, Sheraz Gul, Gurcharan Kaur, Melitta Schachner, Thomas Theis
Polysialic acid (PSA) is a large, negatively charged, linear homopolymer of alpha2-8-linked sialic acid residues. It is generated by two polysialyltransferases and attached to N- and/or O-linked glycans, and its main carrier is the neural cell adhesion molecule NCAM. PSA controls the development and regeneration of the nervous system by enhancing cell migration, axon pathfinding, synaptic targeting, synaptic plasticity, by regulating the differentiation of progenitor cells and by modulating cell-cell and cell-matrix adhesions...
May 24, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28542562/identification-and-characteristics-of-extracellular-vesicles-from-bovine-blastocysts-produced-in-vitro
#4
Edwin A Mellisho, Alejandra E Velásquez, María J Nuñez, Joel G Cabezas, Juan A Cueto, Claudio Fader, Fidel O Castro, Lleretny Rodríguez-Álvarez
Extracellular vesicles (EVs) have been identified within different body fluids and cell culture media. However, there is very little information on the secretion of these vesicles during early embryonic development. The aims of this work were first to demonstrate the secretion of extracellular vesicles by pre-implantation bovine embryos and second to identify and characterize the population of EVs secreted by bovine blastocysts during the period from day seven to nine of embryo culture and its correlation with further embryo development up to day 11...
2017: PloS One
https://www.readbyqxmd.com/read/28537836/recognizing-the-nocebo-benefits-patient-care-but-demands-greater-cultural-competency-in-the-clinic
#5
Antoinette P Joseph, Paul H Mason, Narelle Warren, Isaac Atley
No abstract text is available yet for this article.
June 2017: American Journal of Bioethics: AJOB
https://www.readbyqxmd.com/read/28532700/gastric-cancer-as-preventable-disease
#6
Massimo Rugge, Robert M Genta, Francesco Di Mario, Emad M El-Omar, Hashem B El-Serag, Matteo Fassan, Richard H Hunt, Ernst J Kuipers, Peter Malfertheiner, Kentaro Sugano, David Y Graham
Gastric cancer (GC), one of the five most common causes of cancer death, is associated with a 5-year overall survival rate less than 30%. A minority of cancers occurs as part of syndromic diseases; more than 90% of adenocarcinomas are considered as the ultimate consequence of a longstanding mucosal inflammation. H. pylori infection is the leading etiology of non-self-limiting gastritis, which may result in atrophy of the gastric mucosa and impaired acid secretion. Gastric atrophy establishes a field of cancerization prone to further molecular and phenotypic changes, possibly resulting in cancer growth...
May 19, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28532601/a-replication-competent-foot-and-mouth-disease-virus-expressing-a-luciferase-reporter
#7
Fuquan Zhang, Eva Perez-Martin, Nick Juleff, Bryan Charleston, Julian Seago
Bioluminescence is a powerful tool in the study of viral infection both in vivo and in vitro. Foot-and-mouth disease virus (FMDV) has a small RNA genome with a limited tolerance to foreign RNA entities. There has been no success in making a reporter FMDV expressing a luciferase in infected cell culture supernatants. We report here for the first time a replication-competent FMDV encoding Nanoluciferase, named as Nano-FMDV. Nano-FMDV is genetically stable during serial passages in cells and exhibits growth kinetics and plaque morphology similar to its parental virus...
May 19, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28531268/implementation-of-an-integrated-approach-to-the-national-hiv-aids-strategy-for-improving-human-immunodeficiency-virus-care-for-youths
#8
J Dennis Fortenberry, Linda J Koenig, Bill G Kapogiannis, Carrie L Jeffries, Jonathan M Ellen, Craig M Wilson
Importance: Youths aged 13 to 24 years old living with human immunodeficiency virus (HIV) are less likely than adults to receive the health and prevention benefits of HIV treatments, with only a small proportion having achieved sustained viral suppression. These age-related disparities in HIV continuum of care are owing in part to the unique developmental issues of adolescents and young adults as well as the complexity and fragmentation of HIV care and related services. This article summarizes a national, multiagency, and multilevel approach to HIV care for newly diagnosed youths designed to bridge some of these fragmentations by addressing National HIV/AIDS Strategy goals for people living with HIV...
May 22, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28527789/neural-processes-underlying-cultural-differences-in-cognitive-persistence
#9
Eva H Telzer, Yang Qu, Lynda C Lin
Self-improvement motivation, which occurs when individuals seek to improve upon their competence by gaining new knowledge and improving upon their skills, is critical for cognitive, social, and educational adjustment. While many studies have delineated the neural mechanisms supporting extrinsic motivation induced by monetary rewards, less work has examined the neural processes that support intrinsically motivated behaviors, such as self-improvement motivation. Because cultural groups traditionally vary in terms of their self-improvement motivation, we examined cultural differences in the behavioral and neural processes underlying motivated behaviors during cognitive persistence in the absence of extrinsic rewards...
May 17, 2017: NeuroImage
https://www.readbyqxmd.com/read/28527342/relative-efficacy-of-t-cell-stimuli-as-inducers-of-productive-hiv-1-replication-in-latently-infected-cd4-lymphocytes-from-patients-on-suppressive-cart
#10
Nadejda Beliakova-Bethell, Marjan Hezareh, Joseph K Wong, Matthew C Strain, Mary K Lewinski, Douglas D Richman, Celsa A Spina
Quantification of cell-associated replication-competent HIV, in blood samples from patients with undetectable plasma viremia, requires specialized culture conditions that include exogenous pan T cell stimulation. Different research groups have used several stimuli for this purpose; however, the relative efficacies of these T cell stimuli to induce productive HIV replication from latently infected cells ex vivo have not been systematically evaluated. To this end, we compared four commonly used T cell stimuli: 1) irradiated allogeneic cells plus phytohaemagglutinin (PHA); 2) PHA alone; 3) phorbol myristate acetate plus Ionomycin; and 4) immobilized αCD3 plus αCD28 antibodies...
May 17, 2017: Virology
https://www.readbyqxmd.com/read/28525581/tutorial-speech-assessment-for-multilingual-children-who-do-not-speak-the-same-language-s-as-the-speech-language-pathologist
#11
Sharynne McLeod, Sarah Verdon
Purpose: The aim of this tutorial is to support speech-language pathologists (SLPs) undertaking assessments of multilingual children with suspected speech sound disorders, particularly children who speak languages that are not shared with their SLP. Method: The tutorial was written by the International Expert Panel on Multilingual Children's Speech, which comprises 46 researchers (SLPs, linguists, phoneticians, and speech scientists) who have worked in 43 countries and used 27 languages in professional practice...
May 19, 2017: American Journal of Speech-language Pathology
https://www.readbyqxmd.com/read/28523838/an-unsaturated-quinolone-n-oxide-of-pseudomonas-aeruginosa-modulates-growth-and-virulence-of-staphylococcus-aureus
#12
Dávid Szamosvári, Thomas Böttcher
The pathogen Pseudomonas aeruginosa produces over 50 different quinolones, 16 of which belong to the class of 2-alkyl-4-quinolone N-oxides (AQNOs) with various chain lengths and degrees of saturation. We present the first synthesis of a previously proposed unsaturated compound that is confirmed to be present in culture extracts of P. aeruginosa, and its structure is shown to be trans-Δ(1) -2-(non-1-enyl)-4-quinolone N-oxide. This compound is the most active agent against S.  aureus, including MRSA strains, by more than one order of magnitude whereas its cis isomer is inactive...
May 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28523743/applying-lessons-from-social-psychology-to-transform-the-culture-of-error-disclosure
#13
Jason Han, Denise LaMarra, Neha Vapiwala
CONTEXT: The ability to carry out prompt and effective error disclosure has been described in the literature as an essential skill among physicians that can lead to improved patient satisfaction, staff well-being and hospital outcomes. However, few studies have addressed the social psychology principles that may influence physician behaviour. METHODS: The authors provide an overview of recent administrative measures designed to encourage physicians to disclose error, but note that deliberate practice, buttressed with lessons from social psychology, is needed to implement further productive behavioural changes...
May 18, 2017: Medical Education
https://www.readbyqxmd.com/read/28521749/service-user-involvement-in-mental-health-system-strengthening-in-a-rural-african-setting-qualitative-study
#14
Sisay Abayneh, Heidi Lempp, Atalay Alem, Daniel Alemayehu, Tigist Eshetu, Crick Lund, Maya Semrau, Graham Thornicroft, Charlotte Hanlon
BACKGROUND: It is essential to involve service users in efforts to expand access to mental health care in integrated primary care settings in low- and middle-income countries (LMICs). However, there is little evidence from LMICs to guide this process. The aim of this study was to explore barriers to, and facilitators of, service user/caregiver involvement in rural Ethiopia to inform the development of a scalable approach. METHODS: Thirty nine semi-structured interviews were carried out with purposively selected mental health service users (n = 13), caregivers (n = 10), heads of primary care facilities (n = 8) and policy makers/planners/service developers (n = 8)...
May 18, 2017: BMC Psychiatry
https://www.readbyqxmd.com/read/28521376/-images-of-ageing-in-health-care-magazines-of-the-public-health-system
#15
Ilse Hartmann-Tews, Theresa Hoppe
Objectives Collective images of ageing influence attitudes towards ageing and health- related activities. The aim of this study was to explore images of ageing and old age in magazines published by public health institutions, namely health insurance companies and pharmacies. Design A standardized content analysis was conducted covering age-related articles (n=146) and accompanying photographs (n=218) of public health institutions. The stock of material comprises age-related articles of all magazines published 2012-2013 of 2 public health insurance companies (Allgemeine Ortskrankenkasse: "Bleib gesund", Barmer Ersatzkasse: "Gesundheit konkret"), 2 private health insurance companies (Gothaer Versicherung: "Gothaer magazin", Deutsche Krankenversicherung: "DKV impulse") and 2 consumer magazines of pharmacies ("Apothekenumschau","Senioren Ratgeber")...
May 18, 2017: Das Gesundheitswesen
https://www.readbyqxmd.com/read/28515886/aquatic-microfauna-alter-larval-food-resources-and-affect-development-and-biomass-of-west-nile-and-saint-louis-encephalitis-vector-culex-nigripalpus-diptera-culicidae
#16
Dagne Duguma, Michael G Kaufman, Arthur B Simas Domingos
Ciliate protists and rotifers are ubiquitous in aquatic habitats and can comprise a significant portion of the microbial food resources available to larval mosquitoes, often showing substantial declines in abundance in the presence of mosquito larvae. This top-down regulation of protists is reported to be strong for mosquitoes inhabiting small aquatic containers such as pitcher plants or tree holes, but the nature of these interactions with larval mosquitoes developing in other aquatic habitats is poorly understood...
May 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28515799/implementing-mindfulness-in-the-mainstream-making-the-path-by-walking-it
#17
Rebecca S Crane
There is expanding interest in mindfulness-based programs (MBPs) within the mainstream. While there are research gaps, there is empirical evidence for these developments. Implementing new evidence into practice is always complex and difficult. Particular complexities and tensions arise when implementing MBPs in the mainstream. MBPs are emerging out of the confluence of different epistemologies-contemplative teaching and practice, and contemporary Western empiricism and culture. In the process of navigating implementation and integrity, and developing a professional practice context for this emerging field, the diverse influences within this confluence need careful attention and thought...
2017: Mindfulness
https://www.readbyqxmd.com/read/28515548/culturally-relevant-family-therapy-practice-with-parents-of-children-and-adolescents
#18
Tania Roy, A Thirumoorthy, R Parthasarathy
BACKGROUND: India is diverse in culture, with multiple aspects that may not match with the Western societal picture. Hence, it has often been seen that therapists encounter unique aspects of therapy that is faced during actual practice, which is never written in any textbook or research papers. Substantial information is present through both outcome (efficacy and effectiveness) studies and process research, but it has very little impact of actual Marital and Family Therapy (MFT) practice...
March 2017: Indian Journal of Psychological Medicine
https://www.readbyqxmd.com/read/28514200/culturally-competent-sexual-healthcare-as-a-prerequisite-for-obtaining-preexposure-prophylaxis-findings-from-a-qualitative-study
#19
Kevin M Maloney, Douglas S Krakower, Dale Ziobro, Joshua G Rosenberger, David Novak, Kenneth H Mayer
PURPOSE: Men who have sex with men (MSM) experience stigma in healthcare settings, which impedes disclosure of sexual behavior, potentially limiting uptake of preexposure prophylaxis (PrEP). The purpose of this study was to describe the context of this limitation and explore geographical variability. METHODS: To understand how discomfort in healthcare settings affects PrEP utilization, we conducted two online focus groups with geographically diverse samples of MSM...
May 17, 2017: LGBT Health
https://www.readbyqxmd.com/read/28513216/efficacy-of-syringe-filtration-for-the-selective-isolation-of-campylobacter-from-chicken-carcass-rinse
#20
Jung-Whan Chon, Hong-Seok Kim, Dong-Hyeon Kim, Young-Ji Kim, Kidon Sung, Hyunsook Kim, Kun-Ho Seo
We investigated the efficacy of syringe filtration for selective isolation of Campylobacter from chicken carcass rinse by combining syringe filtration with the conventional culture method. Whole chicken carcass rinses were incubated in Bolton enrichment broth, set aside or subjected to syringe filtration, and streaked on Campy-Cefex agar with or without cefoperazone antibiotic supplement. Compared with the conventional method without filtration, 0.65-μm-pore-size syringe filtration resulted in a significantly higher number of Campylobacter-positive samples (23...
May 17, 2017: Journal of Food Protection
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