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https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#1
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27907984/transnational-collaboration-for-faculty-development-in-health-professions-education-in-mongolia
#2
Hyun Bae Yoon, Jwa-Seop Shin, Seung-Hee Lee, Do-Hwan Kim, Minsun Sung, Nomin Amgalan, Tselmuun Chinzorig
PURPOSE: The Mongolian National University of Medical Sciences is the only national university in Mongolia and has produced more than 90% of health professionals in the country. Experts from Mongolia and Korea embarked on a collaborative effort to develop educational programs for faculty development based on the personal and professional needs of faculty members. This study aimed to evaluate the outcomes of those educational programs to determine whether this transnational collaboration was successful...
December 2016: Korean Journal of Medical Education
https://www.readbyqxmd.com/read/27907898/obstetrical-and-perinatal-outcomes-following-blastocyst-transfer-compared-to-cleavage-transfer-a-systematic-review-and-meta-analysis
#3
W P Martins, C O Nastri, L Rienzi, S Z van der Poel, C R Gracia, C Racowsky
STUDY QUESTION: Is blastocyst transfer safe when compared to cleavage stage embryo transfer regarding obstetric and perinatal outcomes? SUMMARY ANSWER: The clinical equipoise between blastocyst and cleavage stage embryo transfer remains as the evidence associating blastocyst transfer with some adverse perinatal outcomes is of low/very low quality. WHAT IS KNOWN ALREADY: Extended embryo culture to the blastocyst stage provides some theoretical advantages and disadvantages...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27907154/pheromone-recognition-and-selectivity-by-comr-proteins-among-streptococcus-species
#4
Erin Shanker, Donald A Morrison, Antoine Talagas, Sylvie Nessler, Michael J Federle, Gerd Prehna
Natural transformation, or competence, is an ability inherent to bacteria for the uptake of extracellular DNA. This process is central to bacterial evolution and allows for the rapid acquirement of new traits, such as antibiotic resistance in pathogenic microorganisms. For the Gram-positive bacteria genus Streptococcus, genes required for competence are under the regulation of quorum sensing (QS) mediated by peptide pheromones. One such system, ComRS, consists of a peptide (ComS) that is processed (XIP), secreted, and later imported into the cytoplasm, where it binds and activates the transcription factor ComR...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27907039/prosody-predicts-contest-outcome-in-non-verbal-dialogs
#5
Amélie N Dreiss, Philippe G Chatelain, Alexandre Roulin, Heinz Richner
Non-verbal communication has important implications for inter-individual relationships and negotiation success. However, to what extent humans can spontaneously use rhythm and prosody as a sole communication tool is largely unknown. We analysed human ability to resolve a conflict without verbal dialogs, independently of semantics. We invited pairs of subjects to communicate non-verbally using whistle sounds. Along with the production of more whistles, participants unwittingly used a subtle prosodic feature to compete over a resource (ice-cream scoops)...
2016: PloS One
https://www.readbyqxmd.com/read/27907016/direct-unequal-cleavages-embryo-developmental-competence-genetic-constitution-and-clinical-outcome
#6
Qiansheng Zhan, Zhen Ye, Robert Clarke, Zev Rosenwaks, Nikica Zaninovic
OBJECTIVE: To investigate the prevalence, developmental potential, chromosomal constitution and clinical outcome of embryos with direct unequal cleavages (DUC). DESIGN: A retrospective observational study. SETTING: Academic Institution. PARTICIPANT: 21,261 embryos from 3,155 cycles cultured in EmbryoScope®. RESULTS: The total incidence of DUCs per embryo occupying the first three cleavages were 26...
2016: PloS One
https://www.readbyqxmd.com/read/27905279/cloning-of-the-dna-vaccine-candidate-lack-gene-of-leishmania-infantum
#7
Serkan Karaca, Şirin Sahra Ceylan, Süleyman Yazar, Salih Kuk
OBJECTIVE: Leishmaniasis is caused by an obligate intracellular protozoa belonging to Leishmania genus and listed among major tropical diseases by WHO. Because of the high costs, toxicity, and adverse effects of routinely used compounds in the treatment, alternative treatment and vaccine studies are underway. An effective vaccine has not been developed to date. In this study, we aimed to clone one of the most promising DNA vaccine candidates: the homolog-activated C kinase (LACK) gene of Leishmania infantum...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27905177/expressions-of-cultural-safety-in-public-health-nursing-practice
#8
Anna Richardson, Judy Yarwood, Sandra Richardson
Cultural safety is an essential concept within New Zealand nursing that is formally linked to registration and competency-based practice certification. Despite its centrality to New Zealand nursing philosophies and the stated expectation of cultural safety as a practice element, there is limited evidence of its application in the literature. This research presents insight into public health nurse's (PHN) experiences, demonstrating the integration of cultural safety principles into practice. These findings emerged following secondary analysis of data from a collaborative, educative research project where PHNs explored the use of family assessment tools...
November 30, 2016: Nursing Inquiry
https://www.readbyqxmd.com/read/27905152/the-effect-of-cumulus-cells-on-domestic-cat-felis-catus-oocytes-during-in-vitro-maturation-and-fertilization
#9
N Sowińska, K Frankowska, A Filipczyk, A Adamaszek, K Nalik, K Fic, A Pietsch-Fulbiszewska
The aim of this study was to evaluate the effect of co-culture of denuded oocytes with cumulus cells (CC) or cumulus-oocyte complexes (COCs) on in vitro maturation (IVM) and in vitro fertilization (IVF). Immature oocytes were collected from ovaries of domestic cats following a routine ovariectomy. Oocytes were matured in vitro for 24 hr within four groups: (i) denuded oocytes (DO), (ii) DO co-cultured with CC, (iii) DO co-cultured with COC and (iv) COC as a control group. In further experiments, COCs were matured in vitro for 24 hr, and then, oocytes were randomly divided into four groups as previously described and fertilized in vitro...
November 30, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27902947/what-factors-affect-implementation-of-early-rehabilitation-into-intensive-care-unit-practice-a-qualitative-study-with-clinicians
#10
Selina M Parry, Louisa Remedios, Linda Denehy, Laura D Knight, Lisa Beach, Thomas C Rollinson, Sue Berney, Zudin A Puthucheary, Peter Morris, Catherine L Granger
PURPOSE: To identify the barriers and enablers that influence clinicians' implementation of early rehabilitation in critical care. MATERIALS AND METHODS: Qualitative study involving 26 multidisciplinary participants who were recruited using purposive sampling. Four focus groups were conducted using semistructured questions to explore attitudes, beliefs, and experiences. Data were transcribed verbatim and thematic analysis was performed. RESULTS: Six themes emerged, as follows: (1) the clinicians' expectations and knowledge (including rationale for rehabilitation, perceived benefits, and experience), (2) the evidence for and application of rehabilitation (including beliefs regarding when to intervene), (3) patient factors (including prognosis, sedation, delirium, cooperation, motivation, goals, and family), (4) safety considerations (including physiological stability and presence of devices or lines), (5) environmental influences (staffing, resources, equipment, time, and competing priorities), and (6) culture and teamwork...
November 12, 2016: Journal of Critical Care
https://www.readbyqxmd.com/read/27900817/community-based-participatory-research-an-innovative-approach-for-improving-perioperative-care-of-underserved-children
#11
Alvina Rosales, Michelle A Fortier, Belinda Campos, Marla Vivero, Ariana Martinez, Nancy Huerta, Sheeva Zolghadr, Kathleen Adlard, Zeev N Kain
Pediatric disparities disproportionately affect Latino youth undergoing surgery and their families. As such, there is a critical need for culturally relevant frameworks that can advance perioperative intervention approaches in this population and reduce these disparities. In the following article, we first describe the methodological process of community-based participatory research (CBPR) and next report the results of the CBPR process that was conducted in this population. An interdisciplinary group of investigators, Latina mothers, and various other stakeholders met for a series of CBPR-based structured meetings...
November 30, 2016: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/27899268/the-effects-of-the-smart-environment-on-the-information-divide-experienced-by-people-with-disabilities
#12
Su-Jung Nam, Eun-Young Park
BACKGROUND: Information and Communication Technology (ICT) is connected with every aspect of social, cultural, economic, educational, and commercial activity. Smart devices in particular have changed society and are necessary goods for modern people. Smart device usage is rapidly growing in everyday life, so the ability to use a smart device is increasingly important, yet there is little data supporting increased digital inclusion of people with disabilities in mobile device use. OBJECTIVE/HYPOTHESIS: This study investigates the effects of the smart environment on the information divide experienced by people with disabilities...
November 16, 2016: Disability and Health Journal
https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#13
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
November 19, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27896592/why-have-clinical-trials-of-antioxidants-to-prevent-neurodegeneration-failed-a-cellular-investigation-of-novel-phenothiazine-type-antioxidants-reveals-competing-objectives-for-pharmaceutical-neuroprotection
#14
Maike J Ohlow, Selina Sohre, Matthias Granold, Mathias Schreckenberger, Bernd Moosmann
PURPOSE: Only a fraction of the currently established low-molecular weight antioxidants exhibit cytoprotective activity in living cells, which is considered a prerequisite for their potential clinical usefulness in Parkinson's disease or stroke. Post hoc structure-activity relationship analyses have predicted that increased lipophilicity and enhanced radical stabilization could contribute to such cytoprotective activity. METHODS: We have synthesized a series of novel phenothiazine-type antioxidants exhibiting systematic variation in their lipophilicity and radical stabilization...
November 28, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27894355/generation-of-mabs-to-foot-and-mouth-disease-virus-serotype-a-and-application-in-a-competitive-elisa-for-serodiagnosis
#15
Ming Yang, Wanhong Xu, Hilary Bittner, Jacquelyn Horsington, Wilna Vosloo, Melissa Goolia, Diana Lusansky, Charles Nfon
BACKGROUND: Foot-and-mouth disease (FMD) is an economically devastating disease that severely limits international trade of animals. Of the seven FMD virus (FMDV) serotypes, serotype A is one of the most widespread cross the world. Currently antibodies to FMDV are detected in animals using the virus neutralization test (VNT) and the enzyme-linked immunosorbent assay (ELISA). The VNT is laborious, time-consuming and reliant on live virus and cell cultures, while ELISA has the advantage of using inactivated antigens and often provides more reproducible results...
November 28, 2016: Virology Journal
https://www.readbyqxmd.com/read/27893837/cell-type-specific-modulation-of-cobalamin-uptake-by-bovine-serum
#16
Hua Zhao, Kalani Ruberu, Hongyun Li, Brett Garner
Tracking cellular 57Co-labelled cobalamin (57Co-Cbl) uptake is a well-established method for studying Cbl homeostasis. Previous studies established that bovine serum is not generally permissive for cellular Cbl uptake when used as a supplement in cell culture medium, whereas supplementation with human serum promotes cellular Cbl uptake. The underlying reasons for these differences are not fully defined. In the current study we address this question. We extend earlier observations by showing that fetal calf serum inhibits cellular 57Co-Cbl uptake by HT1080 cells (a fibrosarcoma-derived fibroblast cell line)...
2016: PloS One
https://www.readbyqxmd.com/read/27892901/designing-a-virtual-simulation-case-for-cultural-competence-using-a-community-based-participatory-research-approach-a-puerto-rican-case
#17
Lilly Mathew, Barbara B Brewer, Janice D Crist, Robin J Poedel
In this study, a community-based participatory research approach was used for developing content for a virtual simulation case. The virtual simulation case was designed to develop the cultural competence of prelicensure nursing students in caring for a Puerto Rican patient with diabetes. This article presents the method used to establish a Puerto Rican community advisory board to develop content for a virtual simulation case for cultural competency.
October 11, 2016: Nurse Educator
https://www.readbyqxmd.com/read/27890048/cultural-hegemony-educators-perspectives-on-facilitating-cross-cultural-dialogue
#18
Zareen Zaidi, Daniëlle Verstegen, Rashmi Vyas, Omayma Hamed, Tim Dornan, Page Morahan
BACKGROUND: We live in an age when education is being internationalized. This can confront students with 'cultural hegemony' that can result from the unequal distribution of power and privilege in global society. The name that is given to awareness of social inequality is 'critical consciousness'. Cross-cultural dialogue provides an opportunity for learners to develop critical consciousness to counter cultural hegemony. The purpose of this research was to understand how learners engage with cross-cultural dialogue, so we can help them do so more effectively in the future...
2016: Medical Education Online
https://www.readbyqxmd.com/read/27890045/protein-in-culture-and-endogenous-lipid-interact-with-embryonic-stages-in-vitro-to-alter-calf-birthweight-after-embryo-vitrification-and-warming
#19
E Gómez, S Carrocera, S Uzbekova, D Martín, A Murillo, M Alonso-Guervós, F Goyache, M Muñoz
Short-term protein removal in vitro improves long-term blastocyst competence to survive vitrification. We investigated the mechanisms and effects underlying protein removal. Day-6 morulae and early blastocysts were cultured individually with and without protein for 24h. Development and lipid content were analysed in expanded blastocysts derived from morulae (M-XB) and from early blastocysts (EB-XB). Expression of genes involved in lipid metabolism, stress responses and apoptosis was analysed in fresh and vitrified-warmed M-XB produced with and without protein...
November 28, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27889691/development-of-a-culturally-competent-service-to-improve-academic-functioning-for-latino-survivors-of-acute-lymphoblastic-leukemia-methodological-considerations
#20
Laura Bava, Alexis Johns, David R Freyer, Kathleen Ruccione
Many survivors of childhood acute lymphoblastic leukemia (ALL) develop neurocognitive deficits that compromise academic functioning, especially in the presence of sociodemographic risk factors. The extent to which these risk factors coexist for Latino ALL survivors is not well described, but with shifts in U.S. demographics and improved survival in ALL, culturally competent interventions are needed. The Achieving Best Cognitive Successes after Cancer service was designed and implemented by a team representing nursing, medicine, psychology, and social work...
November 25, 2016: Journal of Pediatric Oncology Nursing: Official Journal of the Association of Pediatric Oncology Nurses
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