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https://www.readbyqxmd.com/read/28330195/identification-and-characterization-of-endophytic-bacteria-isolated-from-in-vitro-cultures-of-peach-and-pear-rootstocks
#1
Fakhra Liaqat, Rengin Eltem
Endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. Instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. Five different isolates from peach rootstocks were molecularly identified by 16S rRNA gene sequencing as Brevundimonas diminuta, Leifsonia shinshuensis, Sphingomonas parapaucimobilis Brevundimonas vesicularis, Agrobacterium tumefaciens while two endophytic isolates of pear were identified as Pseudoxanthomonas mexicana, and Stenotrophomonas rhizophilia...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329560/patient-provider-communication-concordance-and-ratings-of-care-in-dermatology-results-of-a-cross-sectional-study
#2
Valerie M Harvey, Uchechukwu Ozoemena, Joan Paul, Hind A Beydoun, Nashay N Clemetson, Ginette A Okoye
ObjectiveTo determine the impact of race concordance on patient perception of quality of dermatologic care.Study designCross-sectional study.SettingAcademic outpatient practices in the Departments of Dermatology of Eastern Virginia Medical School and the Johns Hopkins University School of Medicine.ParticipantsThe study cohort comprised 124 participants including 6 providers and 118 established patients.Main Outcome MeasuresWe hypothesized, a priori, that patients in race-discordant dyads would report lower ratings of participatory decision-making (PDM), satisfaction, trust in the provider, and similarities with providers...
November 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28328934/proviruses-with-identical-sequences-comprise-a-large-fraction-of-the-replication-competent-hiv-reservoir
#3
John K Bui, Michele D Sobolewski, Brandon F Keele, Jonathan Spindler, Andrew Musick, Ann Wiegand, Brian T Luke, Wei Shao, Stephen H Hughes, John M Coffin, Mary F Kearney, John W Mellors
The major obstacle to curing HIV infection is the persistence of cells with intact proviruses that can produce replication-competent virus. This HIV reservoir is believed to exist primarily in CD4+ T-cells and is stable despite years of suppressive antiretroviral therapy. A potential mechanism for HIV persistence is clonal expansion of infected cells, but how often such clones carry replication-competent proviruses has been controversial. Here, we used single-genome sequencing to probe for identical HIV sequence matches among viruses recovered in different viral outgrowth cultures and between the sequences of outgrowth viruses and proviral or intracellular HIV RNA sequences in uncultured blood mononuclear cells from eight donors on suppressive ART with diverse proviral populations...
March 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28327136/perceptions-of-interprofessional-collaboration-of-general-practitioners-and-community-pharmacists-a-qualitative-study
#4
Christin Löffler, Carolin Koudmani, Femke Böhmer, Susanne D Paschka, Jennifer Höck, Eva Drewelow, Martin Stremme, Bernd Stahlhacke, Attila Altiner
BACKGROUND: Despite numerous evidences for the positive effect of community pharmacists on health care, interprofessional collaboration of pharmacists and general practitioners is very often limited. Though highly trained, pharmacists remain an underutilised resource in primary health care in most western countries. This qualitative study aims at investigating pharmacists' and general practitioners' views on barriers to interprofessional collaboration in the German health care system...
March 21, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28323612/from-particularities-to-context-refining-our-thinking-on-illness-narratives
#5
Annie Le, Kara Miller, Juliet McMullin
This paper examines how illness narratives are used in medical education and their implications for clinicians' thinking and care of patients. Ideally, collecting and reading illness narratives can enhance clinicians' sensitivity and contextual thinking. And yet these narratives have become part of institutionalizing cultural competency requirements in ways that tend to favor standardization. Stereotyping and reductionistic thinking can result from these pedagogic approaches and obscure structural inequities...
March 1, 2017: AMA Journal of Ethics
https://www.readbyqxmd.com/read/28323095/megalin-targeted-enhanced-transfection-efficiency-in-cultured-human-hk-2-renal-tubular-proximal-cells-using-aminoglycoside-carboxyalkyl-polyethylenimine-containing-nanoplexes
#6
Fatemeh Oroojalian, Ali Hossein Rezayan, Wayne Thomas Shier, Khalil Abnous, Mohammad Ramezani
Non-viral vectors are of interest as therapeutic gene delivery agents in gene therapy, because they are simple to prepare, easy to modify and have definable safety profiles compared to viral vectors. The potential of gene therapy in the treatment of renal diseases is limited by a lack of effective kidney-targeted gene delivery systems. Aminoglycoside antibiotics gentamicin and neomycin were connected by amide linkages to carboxyl groups on carboxyalkylated-PEI25 (25kDa PEI) or carboxyalkylated-PEI10 (10kDa PEI)...
March 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28320314/quorum-sensing-of-streptococcus-mutans-is-activated-by-aggregatibacter-actinomycetemcomitans-and-by-the-periodontal-microbiome
#7
Szymon P Szafrański, Zhi-Luo Deng, Jürgen Tomasch, Michael Jarek, Sabin Bhuju, Manfred Rohde, Helena Sztajer, Irene Wagner-Döbler
BACKGROUND: The oral cavity is inhabited by complex microbial communities forming biofilms that can cause caries and periodontitis. Cell-cell communication might play an important role in modulating the physiologies of individual species, but evidence so far is limited. RESULTS: Here we demonstrate that a pathogen of the oral cavity, Aggregatibacter actinomycetemcomitans (A. act.), triggers expression of the quorum sensing (QS) regulon of Streptococcus mutans, a well-studied model organism for cariogenic streptococci, in dual-species biofilms grown on artificial saliva...
March 20, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28319583/factor-structure-of-the-cultural-competence-items-in-the-national-home-and-hospice-care-survey
#8
Azza AbuDagga, Constance A Mara, Adam C Carle, Robert Weech-Maldonado
BACKGROUND: There is a need for validated measures of cultural competency practices in home health and hospice care (HHHC). OBJECTIVE: To establish the factor structure of the cultural competency items included in the agency-component of the 2007 public-use National Home and Hospice Care Survey file. DATA SOURCE: We used weighted survey data from 1036 HHHC agencies. RESEARCH DESIGN AND PARTICIPANTS: We used exploratory factor analyses to identify a preliminary factor structure, and then performed confirmatory factor analysis to provide further support for identified factor structure...
March 17, 2017: Medical Care
https://www.readbyqxmd.com/read/28316060/hotair-functions-as-a-competing-endogenous-rna-to-regulate-pten-expression-by-inhibiting-mir-19-in-cardiac-hypertrophy
#9
Yanjun Lai, Shuai He, Liming Ma, Hong Lin, Biyun Ren, Jing Ma, Xinyu Zhu, Shifang Zhuang
Sustained cardiac hypertrophy (CH) is related to a variety of physiological as well as pathological stimuli and eventually increases the risk of heart failure. HOTAIR has been identified as a competing endogenous RNA in multiple human biological processes. Whether lncRNA-HOTAIR is involved in the progress of CH and how it works still remain unknown. Herein, we found that HOTAIR was down-regulated, while miR-19 was up-regulated in both heart tissues from TAC-operated mice in vivo and cultural cardiomyocytes treated with Ang-II in vitro by real-time PCR...
March 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28315858/how-does-culture-affect-experiential-training-feedback-in-exported-canadian-health-professional-curricula
#10
Kerry Wilbur, Rasha Mousa Bacha, Somaia Abdelaziz
Objectives: To explore feedback processes of Western-based health professional student training curricula conducted in an Arab clinical teaching setting. Methods: This qualitative study employed document analysis of in-training evaluation reports (ITERs) used by Canadian nursing, pharmacy, respiratory therapy, paramedic, dental hygiene, and pharmacy technician programs established in Qatar. Six experiential training program coordinators were interviewed between February and May 2016 to explore how national cultural differences are perceived to affect feedback processes between students and clinical supervisors...
March 17, 2017: International Journal of Medical Education
https://www.readbyqxmd.com/read/28314457/transitional-age-lesbian-gay-bisexual-transgender-and-questioning-youth-issues-of-diversity-integrated-identities-and-mental-health
#11
REVIEW
Scott M Rodgers
Although most LGBTQ youth become healthy young adults, they often face considerable stress over the course of their lives because of bullying, victimization, and overt/covert discrimination. Families, educational and religious institutions, health care professionals, and communities help shape the experience of LGBTQ transitional age youth. LGBTQ youth have higher rates of depression, suicide, anxiety, posttraumatic stress disorder, alcohol and drug use, and preventable sexually transmitted diseases. When best practice guidelines are followed and key stakeholders take action to support LGBTQ youth, health disparities begin to disappear...
April 2017: Child and Adolescent Psychiatric Clinics of North America
https://www.readbyqxmd.com/read/28304260/the-challenges-of-cultural-competency-among-expatriate-nurses-working-in-kingdom-of-saudi-arabia
#12
Dalyal N Alosaimi, Muayyad M Ahmad
BACKGROUND AND PURPOSE: To explore the cultural challenges facing expatriate nurses working in the Kingdom of Saudi Arabia (KSA). Women do not have an active role in the workplace in KSA. Concepts of religion, culture, and language are considered to be crucial and often the lack of women's participation is attributed to these factors. METHODS: A descriptive qualitative approach was used with two methods of data collection: interviews and focus group. The study was located in a hospital setting in Al-Riyadh at KSA...
November 1, 2016: Research and Theory for Nursing Practice
https://www.readbyqxmd.com/read/28301300/impact-of-humanitarian-experiences-on-otolaryngology-trainees-a-follow-up-study-of-travel-grant-recipients
#13
Aria Jafari, Kathryn R Tringale, Bruce H Campbell, Jacob W Husseman, Susan R Cordes
In this study, we seek (1) to determine the impact of humanitarian experiences on otolaryngology trainee recipients of the American Academy of Otolaryngology-Head and Neck Surgery Foundation humanitarian travel grant (2001-2015); (2) to better understand trainee and trip characteristics, as well as motivations and attitudes toward future volunteerism; and (3) and to identify potential barriers to participation. An anonymous 30-question survey was distributed to 207 individuals, and 52 (25.1%) responded. Respondents viewed the trip as very worthwhile (score = 98 of 100), expressed improved cultural understanding (75...
February 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28301258/maldi-mass-spectrometry-reveals-that-cumulus-cells-modulate-the-lipid-profile-of-in-vitro-matured-bovine-oocytes
#14
Alessandra A Vireque, Alessandra Tata, Katia Roberta A Belaz, João Gabriel V Grázia, Fábio N Santos, Daniel R Arnold, Andrea C Basso, Marcos N Eberlin, Marcos Felipe Silva-de-Sá, Rui A Ferriani, Ana Carolina J Sá Rosa-E-Silva
The influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squares-discriminant analysis (PLS-DA)...
April 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28299797/processes-that-inform-multicultural-supervision-a-meta-analysis
#15
Nilou B Tohidian, Karen Mui-Teng Quek
As the fields of counseling and psychotherapy have become more cognizant that individuals, couples, and families bring with them a myriad of diversity factors into therapy, multicultural competency has also become a crucial component in the development of clinicians during clinical supervision and training. We employed a qualitative meta-analysis to provide a detailed and comprehensive description of similar themes identified in primary qualitative studies that have investigated supervisory practices with an emphasis on diversity...
March 16, 2017: Journal of Marital and Family Therapy
https://www.readbyqxmd.com/read/28295850/the-effect-of-stereotypes-and-prejudices-regarding-gender-roles-on-the-relation-between-nurses-and-muslim-fathers-in-health-institutions-within-the-community-of-madrid-spain
#16
Juan Luis González-Pascual, Laura Esteban-Gonzalo, Marta Rodríguez-García, Sagrario Gómez-Cantarino, Manuel Moreno-Preciado
Modern Western societies are characterized by a considerable cultural and ethnic diversity whereby different groups and minorities live side by side. However, not all people are viewed in the same light by the autochthonous population. This is particularly true in the case of Muslim immigrants, who are often prone to negative stereotyping and prejudice. This has become increasingly apparent since the 2001 terrorist attacks in New York and the subsequent attacks in various Western countries. This study seeks to analyze the relation between female nurses and those labeled by nurses as "Muslim fathers," as part of a research project on the care of immigrant children in Madrid (Spain)...
March 13, 2017: Nursing Inquiry
https://www.readbyqxmd.com/read/28295279/cultural-competence-among-nursing-students-in-saudi-arabia-a-cross-sectional-study
#17
J P Cruz, N Alquwez, C P Cruz, R F D Felicilda-Reynaldo, L M Vitorino, S M S Islam
AIM: This study assessed the cultural competence of nursing students in a Saudi University. BACKGROUND: With the current situation of immigration in Saudi Arabia, the cultural diversity in healthcare facilities is anticipated to grow. This presents a great challenge to the members of the healthcare team. METHODS: A cross-sectional study was conducted among 272 nursing students in a Saudi university using a self-administered questionnaire consisting of two parts, namely the respondents' demographics and cultural background information sheet and the Cultural Capacity Scale Arabic version...
March 13, 2017: International Nursing Review
https://www.readbyqxmd.com/read/28294309/leadership-behaviours-organizational-culture-and-intention-to-stay-amongst-jordanian-nurses
#18
R F AbuAlRub, M A Nasrallah
AIM: To investigate the impact of leadership behaviours of nurse managers and organizational culture on Jordanian nurses' intention to stay at work in public, private and university hospitals. BACKGROUND: Leadership behaviours of nurses and organizational culture are considered important factors in enhancing retention of nurses. METHODS: A correlational design was used in the study. A sample of 285 Jordanian nurses was conveniently selected to complete a self-administered questionnaire that consisted of three measures; Kouzes and Posner's Leadership Practise Inventory, Professional Organizational Culture questionnaire and McCain's Intent to Stay Scale...
March 15, 2017: International Nursing Review
https://www.readbyqxmd.com/read/28292501/indian-health-service-pharmacists-engaged-in-opioid-safety-initiatives-and-expanding-access-to-naloxone
#19
Hillary Duvivier, Samantha Gustafson, Morgan Greutman, Tenzin Jangchup, Ashlee Knapp Harden, Aimee Reinhard, Keith Warshany
OBJECTIVE: To develop effective pharmacy-based interventions to mitigate harm from opioid use disorders. Programs include responsible opioid prescribing, expanded access to medication-assisted treatment (MAT), naloxone, and community interventions. SETTING: Clinical pharmacists practicing at Indian Health Service (IHS) locations in the Southwest, Midwest, and Great Lakes regions. These pharmacists serve culturally diverse American Indian populations throughout the United States and interface with tribal and federal programs to impact the opioid epidemic in Indian Country...
March 2017: Journal of the American Pharmacists Association: JAPhA
https://www.readbyqxmd.com/read/28292189/translating-agricultural-health-and-medicine-education-across-the-pacific-a-united-states-and-australian-comparison-study
#20
Susan A Brumby, Josie Ruldolphi, Diane Rohlman, Kelley J Donham
BACKGROUND: Populations in agricultural communities require<b> </b>health care that is interdisciplinary and cross-sectoral to address the high rate of workplace deaths, preventable injuries and illness. These rates are compounded by limited access to services and the distinctive personal values and culture of farming populations, which both health and rural practitioners must be aware of to reduce the gap between rural and urban population health outcomes. To address the unique health and medical characteristics of agricultural populations, education in agricultural medicine was established through the College of Medicine and the College of Public Health at the University of Iowa in the USA...
January 2017: Rural and Remote Health
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