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https://www.readbyqxmd.com/read/29785894/developmental-and-molecular-responses-of-buffalo-bubalus-bubalis-cumulus-oocyte-complex-matured-in-vitro-under-heat-shock-conditions
#1
Ashraf El-Sayed, Rehab Nagy, Amal K El-Asheeri, Liala N Eid
SummaryTo investigate the effects of physiologically relevant heat shock during oocyte maturation, buffalo cumulus-oocyte complexes (COCs) were cultured at 38.5°C (control) or were exposed to 39.5°C (T1) or 40.5°C (T2) for the first 6 h of in vitro maturation (IVM), followed by 38.5°C through the next 18 h/IVM and early embryonic development up to the blastocyst stage. Gene expression analysis was performed on selected target genes (HSF-1, HSF-2, HSP-70, HSP-90, BAX, p53, SOD1, COX1, MAPK14) in denuded oocytes and their isolated cumulus cells resulting from control COCs as well as from COCs exposed to a temperature of 39...
May 22, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29784005/cd8-t-cells-mediate-the-antitumor-activity-of-frankincense-and-myrrh-in-hepatocellular-carcinoma
#2
Chun Xu, Xian Lu, Wei Liu, Anxian Chen, Gang Meng, Hailin Zhang, Binghua Li, Yonghui Zhang, Junhua Wu, Jiwu Wei
BACKGROUND: Tumor-promoting inflammation is an emerging hallmark of cancer, which participates in both cancer progression and immune escape. Hepatocellular carcinoma (HCC) is a typical inflammation-related cancer with an extremely poor prognosis. Frankincense and myrrh are anti-inflammation agents commonly used in clinic. The purpose of this study is to investigate whether extract of frankincense and myrrh (FM) downregulates inflammatory microenvironment of HCC and thereby restores antitumor immune responses...
May 21, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29783882/level-of-cultural-self-efficacy-of-colombian-nursing-professionals-and-related-factors
#3
Raquel Herrero-Hahn, Juan Guillermo Rojas, Rafael Montoya-Juárez, María Paz García-Caro, César Hueso-Montoro
PURPOSE: To describe the level of cultural self-efficacy of Colombian nursing professionals and determine the main influencing factors. METHOD: A descriptive, analytical cross-sectional study was conducted in 211 nursing professionals selected by systematic random sampling for a finite population, using the Colombian version of the Cultural Self-Efficacy Scale. After descriptive statistical analysis, nonparametric tests were applied to determine between-variable associations, followed by multiple linear regression analysis...
May 1, 2018: Journal of Transcultural Nursing: Official Journal of the Transcultural Nursing Society
https://www.readbyqxmd.com/read/29783267/-conceptual-approaches-to-the-investigation-of-a-problem-of-professional-training-of-physical-therapists
#4
Anna V Fastivetz, Pavlo V Khomenko, Inna E Shaparenko
OBJECTIVE: Introduction:The level of people's health and its preservation is a medical problem that requires the optimal solution and the need to improve the management of the system of measures to achieve the goal. According to specialists, the health of Ukrainian people is accompanied by high mortality, significant parameters of disability and morbidity and a decrease in the average life expectancy. Diseases that lead to such negative medical and social and socio-economic consequences are so-called "illnesses of a way of life" or "diseases of civilization"...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29783045/factors-affecting-gene-expression-and-activity-of-heme-and-manganese-dependent-catalases-in-lactobacillus-casei-strains
#5
Annamaria Ricciardi, Rocco Gerardo Ianniello, Eugenio Parente, Teresa Zotta
Catalases reduce oxidative stress by degrading hydrogen peroxide to molecular oxygen and water. The presence of heme-dependent or manganese-dependent catalases was observed for a long time in lactic acid bacteria (LAB) but, to date, knowledge on the factors affecting gene expression and enzymatic functionality are limited to a very few strains. In this study, the effect of atmosphere of incubation (not aerated static growth vs aerated shaken growth) and supplementation with Fe2+ , hemin, Mn2+ or their combinations on the catalase production of respiration-competent strain Lactobacillus casei N87 was evaluated using a 24 factorial design...
May 8, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29782496/development-of-a-toolkit-for-piggybac-mediated-integrative-transfection-of-the-human-filarial-parasite-brugia-malayi
#6
Canhui Liu, Amruta S Mhashilkar, Johan Chabanon, Shulin Xu, Sara Lustigman, John H Adams, Thomas R Unnasch
BACKGROUND: The human filarial parasites cause diseases that are among the most important causes of morbidity in the developing world. The elimination programs targeting these infections rely on a limited number of drugs, making the identification of new chemotherapeutic agents a high priority. The study of these parasites has lagged due to the lack of reverse genetic methods. METHODOLOGY/PRINCIPAL FINDINGS: We report a novel co-culture method that results in developmentally competent infective larvae of one of the human filarial parasites (Brugia malayi) and describe a method to efficiently transfect the larval stages of this parasite...
May 21, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29782054/assessment-of-porcine-endogenous-retrovirus-transmission-across-an-alginate-barrier-used-for-the-encapsulation-of-porcine-islets
#7
Claire Crossan, Nizar I Mourad, Karen Smith, Pierre Gianello, Linda Scobie
BACKGROUND: Subcutaneous implantation of a macroencapsulated patch containing human allogenic islets has been successfully used to alleviate type 1 diabetes mellitus (T1DM) in a human recipient without the need for immunosuppression. The use of encapsulated porcine islets to treat T1DM has also been reported. Although no evidence of pathogen transfer using this technology has been reported to date, we deemed it appropriate to determine if the encapsulation technology would prevent the release of virus, in particular, the porcine endogenous retrovirus (PERV)...
May 21, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29781695/meeting-report-how-do-i-incorporate-research-into-my-family-practice-reflections-on-experiences-of-and-solutions-for-young-family-doctors
#8
Kenneth Yakubu, Maria C Colon-Gonzalez, Kyle Hoedebecke, Vasiliki Gkarmiri, Nagwa N Hegazy, Olugbemi O Popoola
BACKGROUND:  Family doctors (FDs) focus on biopsychosocial components of health during consultations. However, much of the evidence employed by these doctors is produced by researchers who are not routinely involved in family practice. Family doctors competent in both clinical practice and research are essential to addressing this gap. With the growing recognition of family medicine as the specialty of choice for many young doctors, there is a scarcity of literature that describes their experiences in combining research and daily family practice...
April 12, 2018: African Journal of Primary Health Care & Family Medicine
https://www.readbyqxmd.com/read/29781633/from-self-construal-to-threat-appraisal-understanding-cultural-differences-in-social-anxiety-between-asian-americans-and-european-americans
#9
Alexander Krieg, Yiyuan Xu
OBJECTIVE: Abundant research has shown that Asian Americans tend to score higher on standardized social anxiety measures than do European Americans. The current study explored how this cultural difference in social anxiety may be explained by cultural differences in self-construals and threat appraisal between Asian Americans and European Americans. METHOD: Participants were 310 Asian Americans and 249 European Americans recruited from a large university in Hawaii who completed questionnaires online in exchange for course credit...
May 21, 2018: Cultural Diversity & Ethnic Minority Psychology
https://www.readbyqxmd.com/read/29780698/multiculturalism-and-interculturalism-redefining-nationhood-and-solidarity
#10
Riva Kastoryano
Theoretical and normative approaches regarding the question of diversity and integration, such as multiucluturalims and interculturalims compete in an attempt to redefine citizenship and nationhood. Most analyses have been single-theory-oriented, leading to multiple, contested and controversial interpretations of integration and democratic public spaces. Transnationalism raises the question of the limits of national public space and extends the concept of cultural integration beyond borders challenging the normative theories of multiculturalism and interculturalism bounded to national societies...
2018: Comparative Migration Studies
https://www.readbyqxmd.com/read/29779723/checking-the-potential-shift-to-perceived-usefulness-the-analysis-of-users-response-to-the-updated-electronic-health-record-core-features
#11
Petar Rajković, Dejan Aleksić, Dragan Janković, Aleksandar Milenković, Ivan Petković
OBJECTIVE: The primary objective of this paper is to identify the main factors which have an impact on the users' attitude towards the functionalities representing the perceived ease of use and to those representing the perceived usefulness. Another objective is to examine whether there occurred a shift towards the perceived usefulness of users' behavior over a period of time. To support these objectives, two different cases are examined - the case in which users are simultaneously offered basic and updated functionalities, and the one in which users are offered a completely new set of features...
July 2018: International Journal of Medical Informatics
https://www.readbyqxmd.com/read/29779553/social-work-student-and-practitioner-roles-in-integrated-care-settings
#12
Erin P Fraher, Erica Lynn Richman, Lisa de Saxe Zerden, Brianna Lombardi
INTRODUCTION: Social workers are increasingly being deployed in integrated medical and behavioral healthcare settings but information about the roles they fill in these settings is not well understood. This study sought to identify the functions that social workers perform in integrated settings and identify where they acquired the necessary skills to perform them. METHODS: Master of social work students (n=21) and their field supervisors (n=21) who were part of a Health Resources and Services Administration-funded program to train and expand the behavioral health workforce in integrated settings were asked how often they engaged in 28 functions, where they learned to perform those functions, and the degree to which their roles overlapped with others on the healthcare team...
June 2018: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/29778986/breaking-through-the-dilemma-of-whether-to-continue-nursing-newly-graduated-nurses-experiences-of-work-challenges
#13
Hwey-Fang Liang, Chun-Chih Lin, Kuang-Ming Wu
BACKGROUND: Newly graduated nurses (NGNs) often encounter unique work challenges. PURPOSE: To discover Taiwanese NGNs' experiences of work challenges. METHOD: An interpretive qualitative design was chosen. In-depth interviews were completed with 25 NGNs working in clinical settings in Taiwan. Data were analyzed using content analysis. RESULTS: The essential structure of NGNs' experiences of work challenges was described as struggling and breaking through the dilemma of deciding whether to continue nursing or quit...
May 4, 2018: Nurse Education Today
https://www.readbyqxmd.com/read/29778905/carbon-dioxide-sensing-in-the-social-context-leaf-cutting-ants-prefer-elevated-co-2-levels-to-tend-their-brood
#14
Daniela Römer, Martin Bollazzi, Flavio Roces
Social insects show temperature and humidity preferences inside their nests to successfully rear brood. In underground nests, ants also encounter rising CO2 concentrations with increasing depth. It is an open question whether they use CO2 as a cue to decide where to place and tend the brood. Leaf-cutting ants do show CO2 preferences for the culturing of their symbiotic fungus. We evaluated their CO2 choices for brood placement in laboratory experiments. Workers of Acromyrmex lundii in the process of relocating brood were offered a binary choice consisting of two interconnected chambers with different CO2 concentrations...
May 17, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29778516/facilitators-of-and-barriers-to-hpv-vaccination-among-sexual-and-gender-minority-patients-at-a-boston-community-health-center
#15
Kaan Z Apaydin, Holly B Fontenot, Derri Shtasel, Sannisha K Dale, Christina P C Borba, Christopher S Lathan, Lori Panther, Kenneth H Mayer, Alex S Keuroghlian
BACKGROUND: Young sexual minority individuals have lower human papillomavirus (HPV) vaccine completion rates than the general population, and little is known about how gender minority people perceive HPV vaccination. The aim of this study was to qualitatively identify patient-, provider-, and systems-level barriers and facilitators for HPV vaccination among sexual and gender minority (SGM) people. METHODS: Fifteen SGM-identified individuals, ages 23-26, were recruited at an urban community health center in Boston, MA, that specializes in care for SGM...
May 16, 2018: Vaccine
https://www.readbyqxmd.com/read/29775994/a-broader-understanding-of-care-managers-attitudes-of-advance-care-planning-a-concurrent-nested-design
#16
Julie Aultman, Kristin R Baughman, Ruth Ludwick
AIMS AND OBJECTIVES: To examine barriers of advance care planning (ACP) experienced by care managers (CMs) through a mixed methods approach. A concurrent nested design was used to acquire a deeper understanding of ACP, and to identify nuances between quantitative and qualitative data. BACKGROUND: Past quantitative studies on providers have identified barriers related to time, culture, knowledge, responsibility, and availability of legal documents. These barriers, and accompanying attitudes and feelings, have been taken at face value without rich qualitative data to identify under what conditions and to what extent a barrier impacts care...
May 18, 2018: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29775509/exploring-the-growth-trajectory-of-cultural-competence-in-taiwanese-paediatric-nurses
#17
Chiu-I Chen, Mei-Chih Huang
AIM: The purpose of this study was to explore the evolution of cultural competence in Taiwanese paediatric nurses. BACKGROUND: Because transnational marriage has become a social phenomenon in Taiwan, the proportion of newborns of new immigrant mothers accounts for 8-10% of total births every year. Since family-centered care is the core value of paediatric nursing, it is necessary to teach caregivers how to take care of hospitalized children and perform related nursing care as well as to determine what difficulties nurses will encounter when they care for patients from diverse cultures and to find solutions for these problems...
May 18, 2018: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29769451/effect-of-ferulic-acid-supplementation-on-the-developmental-competence-of-porcine-embryos-during-in-vitro-maturation
#18
Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano, Toshiki Kunihara, Takeshige Otoi
The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes...
May 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29769080/a-complex-culturally-targeted-intervention-to-reduce-hispanic-disparities-in-living-kidney-donor-transplantation-an-effectiveness-implementation-hybrid-study-protocol
#19
Elisa J Gordon, Jungwha Lee, Raymond H Kang, Juan Carlos Caicedo, Jane L Holl, Daniela P Ladner, Michelle D Shumate
BACKGROUND: The shortage of organs for kidney transplantation for patients with end-stage renal disease (ESRD) is magnified in Hispanics/Latin Americans in the United States. Living donor kidney transplantation (LDKT) is the treatment of choice for ESRD. However, compared to their representation on the transplant waitlist, fewer Hispanics receive a LDKT than non-Hispanic whites. Barriers to LDKT for Hispanics include: lack of knowledge, cultural concerns, and language barriers. Few interventions have been designed to reduce LDKT disparities...
May 16, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29762797/contrasting-incoming-medical-students-attitudes-dual-degree-vs-traditional-tracks
#20
Kristina A Monteiro, Karl Dietrich, Jeffrey Borkan, Luba Dumenco, Allan R Tunkel, Richard Dollase, Paul George
BACKGROUND AND OBJECTIVES: The Warren Alpert Medical School of Brown University (AMS) recently implemented a novel dual degree MD-ScM program in primary care and population medicine (PC-PM) that enrolls up to 24 of its nearly 144 yearly matriculants. The overarching goal of this track is to train medical students to become physician leaders who focus on issues in population medicine within primary care. METHODS: We conducted a baseline assessment of the students enrolled in this parallel track in comparison to our traditional students to identify characteristics of and group differences between students in the PC-PM program and traditional students...
May 2018: Family Medicine
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