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https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#1
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938734/hyperspectral-microscopy-can-detect-metabolic-heterogeneity-within-bovine-post-compaction-embryos-incubated-under-two-oxygen-concentrations-7-versus-20
#2
Melanie L Sutton-McDowall, Martin Gosnell, Ayad G Anwer, Melissa White, Malcolm Purdey, Andrew D Abell, Ewa M Goldys, Jeremy G Thompson
STUDY QUESTION: Can we separate embryos cultured under either 7% or 20% oxygen atmospheres by measuring their metabolic heterogeneity? SUMMARY ANSWER: Metabolic heterogeneity and changes in metabolic profiles in morula exposed to two different oxygen concentrations were not detectable using traditional fluorophore and two-channel autofluorescence but were detectable using hyperspectral microscopy. WHAT IS KNOWN ALREADY: Increased genetic and morphological blastomere heterogeneity is associated with compromised developmental competence of embryos and currently forms the basis for embryo scoring within the clinic...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28936877/botryococcus-as-an-alternative-source-of-carotenoids-and-its-possible-applications-an-overview
#3
Ranga Rao Ambati, Deepika Gogisetty, Ravishankar Aswathnarayana Gokare, Sarada Ravi, Panduranga Narasimharao Bikkina, Yuepeng Su, Bo Lei
The enhanced interest in carotenoid research arises partly because of their application in the food and health industries and partly because of the necessity to find a commercially viable natural source for their mass production. The bottlenecks in finding a natural source of carotenoids which can compete with the synthetic products is the mass production of the organism that produces carotenoids, cell harvesting and extraction methods of carotenoids. The microalga Botryococcus braunii is an interesting organism for its commercial value as a rich source of carotenoids...
September 22, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28936565/development-of-macaque-secondary-follicles-exposed-to-neutral-red-prior-to-3-dimensional-culture
#4
Daiane L Bulgarelli, Alison Y Ting, Brenda J Gordon, Ana Carolina Japur de Sá Rosa-E-Silva, Mary B Zelinski
PURPOSE: Neutral red (NR) may assist identification of preantral follicles in pieces of cortical tissue prior to cryopreservation in cancer patients requesting fertility preservation. This study is the first to analyze this effect by follicle growth rate after long-term culture in primates. METHODS: Ovarian cortex was obtained from adult rhesus macaques, was cut into fragments, and was incubated with NR. Secondary follicles were readily visualized following NR staining and then were encapsulated into alginate beads and cultured individually for 4 weeks in αMEM media supplemented with 10 ng/ml FSH at 5% O2...
September 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28936079/idioms-of-distress
#5
REVIEW
Geetha Desai, Santosh K Chaturvedi
The presentations of psychosocial distress and cultural conflicts are often bodily symptoms, especially in traditional societies and village backgrounds. These might not meet the criteria of the current psychiatric diagnostic systems. Sociocultural milieu contributes to the unique presentations of the stress in the form of idioms of distress. The latter are alternative modes of expressing distress and indicate manifestations of distress in relation to personal and cultural meaning. Health professionals often consider these as hysterical, functional or having functional overlays, and abnormal illness behaviors...
August 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/28935242/supplementation-of-cilostazol-during-in-vitro-maturation-enhances-the-meiosis-and-developmental-competence-of-yak-oocytes-by-influencing-camp-content-and-mrna-expression
#6
Xian-Rong Xiong, Dao-Liang Lan, Jian Li, Ya-Qiu Lin, Ming-Yang Li
The efficiency of in vitro embryo production remains low compared with that observed in vivo. Recent studies have independently shown that cyclic adenosine monophosphate (cAMP) modulation prior to in vitro maturation (IVM) supplementation improves oocyte developmental competence. In this context, special cAMP modulators have been applied during IVM as promising alternatives to improve this biotechnology. Accordingly, this study was conducted to evaluate the effects of treatment with cilostazol, a PDE3 inhibitor, during pre-IVM culture on oocyte meiotic maturation in yak...
August 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28934404/organizational-and-managerial-factors-associated-with-clinical-practice-guideline-adherence-a-simulation-based-study-in-36-french-hospital-wards
#7
Florence Saillour-Glénisson, Marion Kret, Sandrine Domecq, Matthieu Sibé, Valentin Daucourt, Virginie Migeot, David Veillard, Philippe Michel
Objectives: To identify managerial and organizational characteristics of multi-specialty medicine wards and individual characteristics of health professionals that are most strongly associated with clinical practice guidelines (CPG) adherence. Design: Cross-sectional stratified cluster sample design. Setting: Data were gathered from 36 randomly selected multi-specialty medicine wards. Participants: The study population included all health professionals involved in patient care working in the participating wards...
August 1, 2017: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/28931486/caring-for-the-veteran-military-and-family-member-nursing-competencies-strategies-for-integrating-content-into-nursing-school-curricula
#8
Libba Reed McMillan, David Crumbley, Julie Freeman, Marilyn Rhodes, Michele Kane, Joy Napper
In the United States, one in every 15 persons is a Veteran (U. S. Department of Veterans Affairs, 2016; U. S. News & World Report, 2017). An estimated 27% of these Veterans receive healthcare through the Veteran's Health Administration (VHA), leaving 73% to seek care in civilian hospitals (Bagalman, 2014). Realistically, most nurses in the United States will care for military members, Veterans or family members in a variety of healthcare systems and settings. Nurse educators are positioned to lead efforts in providing nursing students with the knowledge necessary to provide competent care and serve as advocates for our nation's heroes...
September 2017: Journal of Professional Nursing: Official Journal of the American Association of Colleges of Nursing
https://www.readbyqxmd.com/read/28930765/encouraging-reflexivity-in-a-residency-leadership-development-program-expanding-outside-the-competency-approach
#9
Justin T Clapp, Emily K B Gordon, Dimitry J Baranov, Beulah Trey, Felice Y Tilin, Lee A Fleisher
PROBLEM: While leadership development is increasingly a goal of academic medicine, it is typically framed as competency acquisition, which can limit its focus to a circumscribed set of social behaviors. This orientation may also reinforce the cultural characteristics of academic medicine that can make effective leadership difficult, rather than training leaders capable of examining and changing this culture. Expanding leadership development so it promotes social reflexivity presents a way to bolster some of the weaknesses of the competency paradigm...
September 14, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28930711/the-angry-amish-grandfather-cultural-competence-and-empathy-a-case-commentary
#10
James L Benedict
Crosscultural encounters are common in the delivery of healthcare, and cultural differences may contribute to misunderstandings and ethical conflict. Encounters between members of the Amish ethno-religious group and modern, science-based healthcare providers hold a high potential for misunderstanding and conflict because the Amish stridently maintain a countercultural outlook and they approach such encounters with suspicion and anxiety. This commentary on the case presented by Amy E. Caruso Brown, MD,1 involving a grandfather's resistance to treating a child with leukemia commends this physician for successfully managing the case and deriving important insights from reflection upon it...
2017: Journal of Clinical Ethics
https://www.readbyqxmd.com/read/28930710/at-the-intersection-of-faith-culture-and-family-dynamics-a-complex-case-of-refusal-of-treatment-for-childhood-cancer
#11
Amy E Caruso Brown
Refusing treatment for potentially curable childhood cancers engenders much discussion and debate. I present a case in which the competent parents of a young Amish child with acute myeloid leukemia deferred authority for decision making to the child's maternal grandfather, who was vocal in his opposition to treatment. I analyze three related concerns that distinguish this case from other accounts of refused treatment. First, I place deference to grandparents as decision makers in the context of surrogate decision making more generally...
2017: Journal of Clinical Ethics
https://www.readbyqxmd.com/read/28930638/use-of-telehealth-for-health-care-of-indigenous-peoples-with-chronic-conditions-a-systematic-review
#12
Sarah Fraser, Tamara Mackean, Julian Grant, Kate Hunter, Kurt Towers, Rebecca Ivers
INTRODUCTION: Telehealth may be a cost effective modality in healthcare delivery, but how well used or how appropriate it is for the care of Indigenous peoples is unclear. This review examines the evidence for telehealth in facilitating chronic conditions management with Indigenous peoples. METHODS: Databases were systematically searched for qualitative or quantitative primary research studies that investigated telehealth use for chronic conditions management with Indigenous peoples worldwide...
July 2017: Rural and Remote Health
https://www.readbyqxmd.com/read/28929848/the-leadership-shift-from-directing-people-to-action-to-inspiring-people-to-action
#13
Bruce Harry Swan
The leadership shift from directing people to action to inspiring people to action will focus on the coach approach to leadership and will clearly demonstrate how taking this approach not only gives lasting cultural change but also builds leadership capacity. Current examples of healthcare organizations that have implemented this approach will be shared along with the use of best practices coaching skills that embrace the International Coach Federation core competencies and code of ethics.
September 2017: Healthcare Management Forum
https://www.readbyqxmd.com/read/28928722/fermentative-bacteria-influence-the-competition-between-denitrifiers-and-dnra-bacteria
#14
Eveline M van den Berg, Marina P Elisário, J Gijs Kuenen, Robbert Kleerebezem, Mark C M van Loosdrecht
Denitrification and dissimilatory reduction to ammonium (DNRA) are competing nitrate-reduction processes that entail important biogeochemical consequences for nitrogen retention/removal in natural and man-made ecosystems. The nature of the available carbon source and electron donor have been suggested to play an important role on the outcome of this microbial competition. In this study, the influence of lactate as fermentable carbon source on the competition for nitrate was investigated for varying ratios of lactate and nitrate in the influent (Lac/N ratio)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28927798/effect-of-charcoal-dextran-stripped-fetal-bovine-serum-on-in-vitro-development-of-bovine-embryos
#15
Ayman Mesalam, Rami Kong, Imran Khan, Mmr Chowdhury, Byung-Hyun Choi, Sung Woo Kim, Kyu-Woan Cho, Jong-In Jin, Il-Keun Kong
This study investigated the ability of charcoal:dextran stripped fetal bovine serum (CDS FBS) and heat-inactivated fetal bovine serum (HI FBS) to support in vitro development of bovine embryos. The developmental ability and quality of bovine embryos were determined by assessing their cell number, lipid content, mitochondrial activity, gene expression, and cryo-tolerance. The percentage of embryos that formed a blastocyst was significantly (P<0.05) higher in medium containing CDS FBS than in medium containing HI FBS (42...
September 16, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28927332/qualitative-and-quantitative-feedback-in-the-context-of-competency-based-education
#16
Ara Tekian, Christopher J Watling, Trudie E Roberts, Yvonne Steinert, John Norcini
Research indicates the importance and usefulness of feedback, yet with the shift of medical curricula toward competencies, feedback is not well understood in this context. This paper attempts to identify how feedback fits within a competency-based curriculum. After careful consideration of the literature, the following conclusions are drawn: (1) Because feedback is predicated on assessment, the assessment should be designed to optimize and prevent inaccuracies in feedback; (2) Giving qualitative feedback in the form of a conversation would lend credibility to the feedback, address emotional obstacles and create a context in which feedback is comfortable; (3) Quantitative feedback in the form of individualized data could fulfill the demand for more feedback, help students devise strategies on how to improve, allow students to compare themselves to their peers, recognizing that big data have limitations; and (4) Faculty development needs to incorporate and promote cultural and systems changes with regard to feedback...
September 19, 2017: Medical Teacher
https://www.readbyqxmd.com/read/28922408/the-role-of-the-pi3k-akt-signaling-pathway-in-the-developmental-competence-of-bovine-oocytes
#17
Gabriella Mamede Andrade, Juliano Coelho da Silveira, Claudia Perrini, Maite Del Collado, Samuel Gebremedhn, Dawit Tesfaye, Flávio Vieira Meirelles, Felipe Perecin
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging "messages". We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation...
2017: PloS One
https://www.readbyqxmd.com/read/28919831/flipped-classroom-model-for-learning-evidence-based-medicine
#18
Sydney Y Rucker, Zulfukar Ozdogan, Morhaf Al Achkar
Journal club (JC), as a pedagogical strategy, has long been used in graduate medical education (GME). As evidence-based medicine (EBM) becomes a mainstay in GME, traditional models of JC present a number of insufficiencies and call for novel models of instruction. A flipped classroom model appears to be an ideal strategy to meet the demands to connect evidence to practice while creating engaged, culturally competent, and technologically literate physicians. In this article, we describe a novel model of flipped classroom in JC...
2017: Advances in Medical Education and Practice
https://www.readbyqxmd.com/read/28918704/professionalism-among-multicultural-medical-students-in-the-united-arab-emirates
#19
Mahera Abdulrahman, Shahd Alsalehi, Zahra S M Husain, Satish C Nair, Frederick Robert Carrick
BACKGROUND: Moral competencies and ethical practices of medical professionals are among the desired outcomes of academic training. Unfortunately, academic dishonesty and misconduct are reported from medical colleges across the world. This study investigates the level of academic dishonesty/misconduct among multicultural medical students. OBJECTIVE: The aim of this study is to investigate the level of academic dishonesty/misconduct among multicultural medical students...
2017: Medical Education Online
https://www.readbyqxmd.com/read/28915817/cultural-adaptation-of-a-pediatric-functional-assessment-for-rehabilitation-outcomes-research
#20
Kristen E Arestad, David MacPhee, Chun Y Lim, Mary A Khetani
BACKGROUND: Significant racial and ethnic health care disparities experienced by Hispanic children with special health care needs (CSHCN) create barriers to enacting culturally competent rehabilitation services. One way to minimize the impact of disparities in rehabilitation is to equip practitioners with culturally relevant functional assessments to accurately determine service needs. Current approaches to culturally adapting assessments have three major limitations: use of inconsistent translation processes; current processes assess for some, but not all, elements of cultural equivalence; and limited evidence to guide decision making about whether to undertake cultural adaptation with and without language translation...
September 15, 2017: BMC Health Services Research
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