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https://www.readbyqxmd.com/read/28193729/sequential-divergent-and-cooperative-requirements-of-foxl2a-and-foxl2b-in-ovary-development-and-maintenance-of-zebrafish
#1
Yan-Jing Yang, Yang Wang, Zhi Li, Li Zhou, Jian-Fang Gui
Foxl2 is essential for mammalian ovary maintenance. Although sexually dimorphic expression of foxl2 was observed in many teleost, its role and regulative mechanism in fish remained largely unclear. In this study, we first identified two transcript variants of foxl2a and its homologous gene foxl2b in zebrafish, and revealed their specific expression in follicular layer cells in a sequential and divergent fashion during ovary differentiation, maturation and maintenance. Then, homozygous foxl2a mutants (foxl2a(-/-)) and foxl2b mutants (foxl2b(-/-)) were constructed, and detailed comparisons, such as sex ratio, gonadal histological structure, transcriptome profiling and dynamic expression of gonadal development-related genes, were carried out...
February 13, 2017: Genetics
https://www.readbyqxmd.com/read/28118999/reproductive-and-transcriptional-effects-of-the-antiandrogenic-progestin-chlormadinone-acetate-in-zebrafish-danio-rerio
#2
Patricia Franziska Siegenthaler, Yanbin Zhao, Kun Zhang, Karl Fent
Chlormadinone acetate (CMA) is a frequently used progestin with antiandrogenic activity in humans. Residues may enter the aquatic environment but potential adverse effects in fish are unknown. While our previous work focused on effects of CMA in vitro and in zebrafish eleuthero-embryos, the present study reports on reproductive and transcriptional effects in adult female and male zebrafish (Danio rerio). We performed a reproductive study using breeding groups of zebrafish. After 15 days of pre-exposure, we exposed zebrafish to different measured concentrations between 6...
January 21, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28013034/spatiotemporal-expression-analysis-of-nuclear-estrogen-receptors-in-the-zebrafish-ovary-and-their-regulation-in-vitro-by-endocrine-hormones-and-paracrine-factors
#3
Ka-Cheuk Liu, Shuk-Wa Lau, Wei Ge
Estradiol (E2) stimulates luteinizing hormone receptor (lhcgr) expression via nuclear estrogen receptors (nERs) in the zebrafish ovary. We have demonstrated that endocrine hormones such as gonadotropin (hCG) and paracrine factors such as epidermal growth factor (EGF) and pituitary adenylate cyclase-activating peptide (PACAP) can modulate E2-induced lhcgr expression in vitro. These observations raised a question on whether these hormones and factors exert their effects via regulating the expression of nERs. In this study, we first characterized the spatiotemporal expression profiles of three nER subtypes in the zebrafish ovary, including esr1 (ERα), esr2a (ERβ2) and esr2b (ERβ1)...
December 21, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28002497/a-novel-igf3-gene-in-common-carp-cyprinus-carpio-evidence-for-its-role-in-regulating-gonadal-development
#4
Feibiao Song, Lanmei Wang, Wenbin Zhu, Jianjun Fu, Juanjuan Dong, Zaijie Dong
Since the insulin-like growth factor 3 (igf3) gene was recently discovered in fish ovary, its function in the gonads has received much attention. In this study, we isolated two igf3 subtypes from common carp (Cyprinus carpio), which comprised full-length cDNA of 707 and 1153 nucleotides encoding 205 and 198 amino acids (aa), respectively. The Igf3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish (Danio rerio). Phylogenetic tree construction revealed that the C. carpio igf3 gene was first clustered with D...
2016: PloS One
https://www.readbyqxmd.com/read/27988227/methods-for-the-analysis-of-early-oogenesis-in-zebrafish
#5
Yaniv M Elkouby, Mary C Mullins
Oocyte differentiation is a highly dynamic and intricate developmental process whose mechanistic understanding advances female reproduction, fertility, and ovarian cancer biology. Despite the many attributes of the zebrafish model, it has yet to be fully exploited for the investigation of early oocyte differentiation and ovarian development. This is partly because the properties of the adult zebrafish ovary make it technically challenging to access early stage oocytes. As a result, characterization of these stages has been lacking and tools for their analysis have been insufficient...
December 14, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27940159/dmrt1-is-necessary-for-male-sexual-development-in-zebrafish
#6
Kaitlyn A Webster, Ursula Schach, Angel Ordaz, Jocelyn S Steinfeld, Bruce W Draper, Kellee R Siegfried
The dmrt1 (doublesex and mab-3 related transcription factor 1) gene is a key regulator of sex determination and/or gonadal sex differentiation across metazoan animals. This is unusual given that sex determination genes are typically not well conserved. The mechanisms by which zebrafish sex is determined have remained elusive due to the lack of sex chromosomes and the complex polygenic nature of sex determination in domesticated strains. To investigate the role of dmrt1 in zebrafish sex determination and gonad development, we isolated mutations disrupting this gene...
December 8, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27913641/translation-repression-by-maternal-rna-binding-protein-zar1-is-essential-for-early-oogenesis-in-zebrafish
#7
Liyun Miao, Yue Yuan, Feng Cheng, Junshun Fang, Fang Zhou, Weirui Ma, Yan Jiang, Xiahe Huang, Yingchun Wang, Lingjuan Shan, Dahua Chen, Jian Zhang
Large numbers of maternal RNAs are deposited in oocytes and are reserved for later development. Control of maternal RNA translation during oocyte maturation has been extensively investigated and its regulatory mechanisms are well documented. However, translational regulation of maternal RNAs in early oogenesis is largely unexplored. In this study, we generated zebrafish zar1 mutants which result in early oocyte apoptosis and fully penetrant male development. Loss of p53 suppresses the apoptosis in zar1 mutants and restores oocyte development...
December 2, 2016: Development
https://www.readbyqxmd.com/read/27876832/knockout-of-zebrafish-ovarian-aromatase-gene-cyp19a1a-by-talen-and-crispr-cas9-leads-to-all-male-offspring-due-to-failed-ovarian-differentiation
#8
Esther Shuk-Wa Lau, Zhiwei Zhang, Mingming Qin, Wei Ge
Sexual or gonadal differentiation is a complex event and its mechanism remains elusive in teleosts. Despite its complexity and plasticity, the process of ovarian differentiation is believed to involve gonadal aromatase (cyp19a1a) in nearly all species studied. However, most data concerning the role of aromatase have come from gene expression analysis or studies involving pharmacological approaches. There has been a lack of genetic evidence for the importance of aromatase in gonadal differentiation, especially the timing when the enzyme starts to exert its effect...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27734351/identification-of-germ-line-stem-cells-in-zebrafish
#9
Bruce W Draper
Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27686790/identification-of-candidate-pirnas-in-the-gonads-of-paralichthys-olivaceus-japanese-flounder
#10
Chun-Lei Wang, Zhi-Peng Wang, Jia-Qi Wang, Ming-You Li, Xiao-Wu Chen
Piwi-interacting RNA (piRNA) plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder (Paralichthys olivaceus) were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters (PRCs) were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found...
September 18, 2016: Zool Res
https://www.readbyqxmd.com/read/27664022/different-effects-of-blue-and-red-light-emitting-diodes-on-antioxidant-responses-in-the-liver-and-ovary-of-zebrafish-danio-rerio
#11
Shuang-Shuang Yuan, Huan-Zhi Xu, Li-Qin Liu, Jia-Lang Zheng
The present study assessed the effects of a white fluorescent bulb (the control) and two different light-emitting diodes (blue LEDs, LDB; red, LDR) on growth, morphology, and oxidative stress in the liver and ovary of zebrafish for 5 weeks. Growth maintained relatively constant under LDB condition, but was reduced under LDR condition. In the liver, hepatosomatic index (HSI) and protein carbonylation (PC) increased under LDR condition, whereas lipid peroxidation (LPO) declined and HSI remained unchanged under LDB condition...
September 23, 2016: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/27642707/acute-exposure-to-waterborne-cadmium-induced-oxidative-stress-and-immunotoxicity-in-the-brain-ovary-and-liver-of-zebrafish-danio-rerio
#12
Jia-Lang Zheng, Shuang-Shuang Yuan, Chang-Wen Wu, Zhen-Ming Lv
Cadmium (Cd) is an environmental contaminant that poses serious risks to aquatic organisms and their associated ecosystem. The mechanisms underlying Cd-induced oxidative stress and immunotoxicity in fish remain largely unknown. In this study, adult female zebrafish were exposed to 0 (control), 1mgL(-1) Cd for 24h and 96h, and the oxidative stress and inflammatory responses induced by Cd were evaluated in the brain, liver and ovary. Reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA) increased in a time-dependent manner after treatment with Cd in the brain and liver...
November 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27618422/corticosteroid-fludrocortisone-acetate-targets-multiple-end-points-in-zebrafish-danio-rerio-at-low-concentrations
#13
Yanbin Zhao, Kun Zhang, Karl Fent
Synthetic corticosteroids may pose an environmental risk to fish. Here, we describe multiend point responses of adult zebrafish (8 months old) upon 21-day exposure to a commonly prescribed corticosteroid, fludrocortisone acetate (FLU), at concentrations between 0.006 and 42 μg/L. No remarkable reproductive impacts were observed, while physiological effects, including plasma glucose level and blood leukocyte numbers were significant altered even at 42 ng/L. Ovary parameters and transcriptional analysis of hypothalamic-pituitary-gonadal-liver axis revealed negligible effects...
September 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27616647/reproductive-toxicity-of-azoxystrobin-to-adult-zebrafish-danio-rerio
#14
Fangjie Cao, Lizhen Zhu, Hui Li, Song Yu, Chengju Wang, Lihong Qiu
In the past few decades, extensive application of azoxystrobin has led to great concern regarding its adverse effects on aquatic organisms. The objective of the present study was to evaluate the reproductive toxicity of azoxystrobin to zebrafish. After adult zebrafish of both sexes were exposed to 2, 20 and 200 μg/L azoxystrobin for 21 days, egg production, the fertilization rate, the gonadosomatic index (GSI) and hepatosomatic index (HSI), 17β-estradiol (E2), testosterone (T) and vitellogenin (Vtg) concentrations, and histological alterations in the gonads and livers were measured...
December 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27580802/neurokinin-b-exerts-direct-effects-on-the-ovary-to-stimulate-estradiol-production
#15
Xin Qi, Mohamed Salem, Wenyi Zhou, Miwa Sato-Shimizu, Gang Ye, Johan Smitz, Chun Peng
Neurokinin B (NKB) and its receptor, NK3R, play critical roles in reproduction by regulating the secretion of the hypothalamic GnRH. NKB and NK3R genes are also expressed in the ovary; however, their physiological roles within the ovary are unknown. The aim of this study was to determine whether NKB acts directly on the ovary to regulate reproduction. Injection of NKB into zebrafish accelerated follicle development, increased the mRNA levels of cyp11a1 and cyp19a1, and enhanced estradiol production. Similarly, NKB induced cyp11a1 and cyp19a1 expression in primary cultures of zebrafish follicular cells and stimulated estradiol production from cultured follicles...
September 2016: Endocrinology
https://www.readbyqxmd.com/read/27574813/effects-of-%C3%AE-diketone-antibiotics-on-f1-zebrafish-danio-rerio-based-on-high-throughput-mirna-sequencing-under-exposure-to-parents
#16
Yuansi Zheng, Jiebo Lin, Jieyi Li, Haifeng Zhang, Weiming Ai, Xuedong Wang, Randy A Dahlgren, Huili Wang
The toxicity of β-diketone antibiotics (DKAs), a class of ''pseudo-persistent'' environmental pollutants, to F0-zebrafish (Danio rerio) was investigated using 7-dpf F1-zebrafish miRNA sequencing and bioinformatics analyses. Based on relative expression, 47, 134 and 118 of 193 mature miRNAs were differentially expressed between control vs 6.25 mg/L, control vs 12.5 mg/L and 6.25 vs 12.5 mg/L treatments, respectively. Utilizing three databases, 2523 potential target genes were predicted, and they were assigned to 19 high-abundance KEGG pathways and 20 functional categories by COG analysis...
December 2016: Chemosphere
https://www.readbyqxmd.com/read/27571716/effects-of-azocyclotin-on-gene-transcription-and-steroid-metabolome-of-hypothalamic-pituitary-gonad-axis-and-their-consequences-on-reproduction-in-zebrafish-danio-rerio
#17
You-Ning Ma, Chu-Yan Cao, Qiang-Wei Wang, Wen-Jun Gui, Guo-Nian Zhu
The widely used organotins have the potential to disrupt the endocrine system, but little is known of underlying mechanisms of azocyclotin toxicity in fish. The objective of the present study was to investigate the impact of azocyclotin on reproduction in zebrafish. Adult zebrafish were exposed to 0.09 and 0.45μg/L azocyclotin for 21days, and effects on steroid hormones and mRNA expression of the genes belonging to the hypothalamic-pituitary-gonad (HPG) axis were investigated. Mass spectrometry methodology was developed to profile steroids within the metabolome of the gonads...
October 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27452972/positive-and-negative-innate-immune-responses-in-zebrafish-under-light-emitting-diodes-conditions
#18
Jia-Lang Zheng, Shuang-Shuang Yuan, Wei-Ye Li, Chang-Wen Wu
Certain light emitting diodes (LEDs) have become popular in fish farming beacause of a promoting effect on growth and reproduction. However, little information is available on innate immune responses in related tissues under LEDs conditions. The present study assessed the effects of a white fluorescent bulb (the control) and two different light-emitting diodes (LEDs: blue, LDB, peak at 450 nm; red, LDR, 630 nm) on growth and innate immune responses in the serum, liver and ovary of zebrafish for 8 weeks. LDB significantly enhanced specific growth rate (SGR), food intake (FI), and serum globulin levels...
September 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27446014/liposome-mediated-herpes-simplex-virus-uptake-is-glycoprotein-d-receptor-independent-but-requires-heparan-sulfate
#19
Lorrie A Burnham, Dinesh Jaishankar, Jeffrey M Thompson, Kevin S Jones, Deepak Shukla, Vaibhav Tiwari
Cationic liposomes are widely used to facilitate introduction of genetic material into target cells during transfection. This study describes a non-receptor mediated herpes simplex virus type-1 (HSV-1) entry into the Chinese hamster ovary (CHO-K1) cells that naturally lack glycoprotein D (gD)-receptors using a commercially available cationic liposome: lipofectamine. Presence of cell surface heparan sulfate (HS) increased the levels of viral entry indicating a potential role of HS in this mode of entry. Loss of viral entry in the presence of actin de-polymerizing or lysosomotropic agents suggests that this mode of entry results in the endocytosis of the lipofectamine-virus mixture...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27391360/reproductive-toxicity-of-inorganic-mercury-exposure-in-adult-zebrafish-histological-damage-oxidative-stress-and-alterations-of-sex-hormone-and-gene-expression-in-the-hypothalamic-pituitary-gonadal-axis
#20
Qun-Fang Zhang, Ying-Wen Li, Zhi-Hao Liu, Qi-Liang Chen
Mercury (Hg) is a prominent environmental contaminant that causes a variety of adverse effects on aquatic organisms. However, the mechanisms underlying inorganic Hg-induced reproductive impairment in fish remains largely unknown. In this study, adult zebrafish were exposed to 0 (control), 15 and 30μg Hg/l (added as mercuric chloride, HgCl2) for 30days, and the effects on histological structure, antioxidant status and sex hormone levels in the ovary and testis, as well as the mRNA expression of genes involved in the hypothalamic-pituitary-gonadal (HPG) axis were analyzed...
August 2016: Aquatic Toxicology
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