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https://www.readbyqxmd.com/read/29096293/effects-of-the-bioconcentration-and-parental-transfer-of-environmentally-relevant-concentrations-of-difenoconazole-on-endocrine-disruption-in-zebrafish-danio-rerio
#1
Miaomiao Teng, Suzhen Qi, Wentao Zhu, Yao Wang, Dezhen Wang, Kai Dong, Chengju Wang
Difenoconazole, a typical triazole fungicide, inhibits lanosterol-14R-demethylase (CYP51) to prevent fungal sterol synthesis and its residues are frequently detected in the environment due to its wide application. Previous studies have demonstrated that difenoconazole altered the triglyceride levels, and gene expression relevant to cholesterol biosynthesis in zebrafish. However, endocrine-disruption in the hypothalamus-pituitary-gonadal-liver (HPGL) axis, the effects of transferring to offspring, and the underlying mechanisms of difenoconazole in aquatic organisms are still unknown...
October 26, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29055862/juvenile-exposure-to-bisphenol-a-promotes-ovarian-differentiation-but-suppresses-its-growth-potential-involvement-of-pituitary-follicle-stimulating-hormone
#2
Weiting Chen, Shuk-Wa Lau, Yuqin Fan, Rudolf S S Wu, Wei Ge
Bisphenol A (BPA), a plastic monomer and plasticizer, is commonly used in plastics industry, and it has been well documented to be an estrogenic endocrine disrupter. In the present study, we investigated the effect of early (juvenile) exposure to BPA on the hypothalamic-pituitary-gonad (HPG) axis in the zebrafish. Estradiol (E2) and testosterone (T) were also included as positive and negative controls respectively. Juvenile zebrafish were exposed to BPA (1 and 10μM), E2 (10nM) and T (10nM) from 20 to 40 dpf (days post-fertilization), the period of sex/gonadal differentiation, followed by histological and expression analyses at 40 dpf...
October 14, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29051429/fish-reproduction-is-disrupted-upon-lifelong-exposure-to-environmental-pahs-fractions-revealing-different-modes-of-action
#3
Caroline Vignet, Thibaut Larcher, Blandine Davail, Lucette Joassard, Karyn Le Menach, Tiphaine Guionnet, Laura Lyphout, Mireille Ledevin, Manon Goubeau, Hélène Budzinski, Marie-Laure Bégout, Xavier Cousin
Polycyclic aromatic hydrocarbons (PAHs) constitute a large family of organic pollutants emitted in the environment as complex mixtures, the compositions of which depend on origin. Among a wide range of physiological defects, PAHs are suspected to be involved in disruption of reproduction. In an aquatic environment, the trophic route is an important source of chronic exposure to PAHs. Here, we performed trophic exposure of zebrafish to three fractions of different origin, one pyrolytic and two petrogenic. Produced diets contained PAHs at environmental concentrations...
October 28, 2016: Toxics
https://www.readbyqxmd.com/read/29024578/morpho-functional-features-of-the-gonads-of-danio-rerio-the-role-of-brain-derived-neurotrophic-factor
#4
Pietro Cacialli, Livia D'Angelo, Paolo de Girolamo, Luigi Avallone, Carla Lucini, Elisabeth Pellegrini, Luciana Castaldo
Zebrafish, a suitable and widely used teleost fish model in basic biomedical research, displays morphophysiological features of adult gonads that share some commonalities with those of mammalian species. In mammals, gametogenesis is regulated, among several factors, by brain-derived neurotrophic factor (BDNF). This neurotrophin has a well-established role in the developing and adult nervous system, as well as gonads development and functions in vertebrate species. We hypothesize that BDNF has a role also in the gonadal functions of zebrafish...
October 12, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28972919/reproductive-effects-of-synthetic-progestin-norgestrel-in-zebrafish-danio-rerio
#5
Yan-Qiu Liang, Guo-Yong Huang, Zhong Lin, Jin Li, Jie-Wen Yang, Lai-Yuan Zhong, Guang-Guo Ying
The aim of this study was to assess the adverse effects of synthetic progestin norgestrel (NGT) on the reproduction of zebrafish by measuring the egg production, histology and transcriptional expression profiles along the hypothalamic-pituitary-gonadal (HPG) axis in adult zebrafish. After a pre-exposure period of 7 days, adult zebrafish were exposed to 6, 29 and 69 ng L(-1) NGT for 21 days. The results showed that exposure to 69 ng L(-1) NGT led to a significant up-regulation of follicle stimulating hormone, beta polypeptide (fshb), luteinizing hormone, beta polypeptide (lhb), progesterone receptor (pgr), estrogen receptor 1 (esr1) and androgen receptor (ar) genes in the brains, as well as significant up-regulation of hydroxysteroid 20-beta dehydrogenase (hsd20b) and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2) genes and down-regulation of 11-beta-hydroxylase (cyp11b) gene in the ovaries of females...
September 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/28922741/genomic-and-biochemical-characterization-of-sialic-acid-acetylesterase-siae-in-zebrafish
#6
Viola Ravasio, Eufemia Damiati, Daniela Zizioli, Flavia Orizio, Edoardo Giacopuzzi, Marta Manzoni, Roberto Bresciani, Giuseppe Borsani, Eugenio Monti
Sialic acid acetylesterase (SIAE) removes acetyl moieties from the carbon 9 and 4 hydroxyl groups of sialic acid and recently a debate has been opened on its association to autoimmunity. Trying to get new insights on this intriguing enzyme we have studied siae in zebrafish (Danio rerio). In this teleost siae encodes for a polypeptide with a high degree of sequence identity to human and mouse counterparts. Zebrafish Siae behavior upon transient expression in COS7 cells is comparable to human enzyme concerning pH optimum of enzyme activity, subcellular localization and glycosylation...
October 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28873404/fibroblast-growth-factor-signaling-is-required-for-early-somatic-gonad-development-in-zebrafish
#7
Dena M Leerberg, Kaori Sano, Bruce W Draper
The vertebrate ovary and testis develop from a sexually indifferent gonad. During early development of the organism, primordial germ cells (the gamete lineage) and somatic gonad cells coalesce and begin to undergo growth and morphogenesis to form this bipotential gonad. Although this aspect of development is requisite for a fertile adult, little is known about the genetic regulation of early gonadogenesis in any vertebrate. Here, we provide evidence that fibroblast growth factor (Fgf) signaling is required for the early growth phase of a vertebrate bipotential gonad...
September 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28831411/whole-transcriptome-data-of-zebrafish-exposed-to-chronic-dose-of-depleted-uranium
#8
Olivier Armant, Kewin Gombeau, Christelle Adam Guillermin
The concentration of depleted uranium (DU) in the environment is expected to increase due to anthropogenic activities, posing potential risks on ecosystems. The effects of chronic exposure to DU at concentration close to the environmental standards (0.3-30 µg DU/L) are scarcely characterised. Genomic alterations caused by low doses of pollutants can potentially propagate over generations, but how these effects may affect the health of the progeny remain uncertain for the vast majority of toxicants. The present dataset describes the transcriptomic effects of a chronic exposure to 20 µg DU/L during 10 days on adult zebrafish (Danio rerio) organs, the brain, the testis and the ovaries...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28791769/fluorescent-polymer-nanoparticles-for-cell-barcoding-in-vitro-and-in-vivo
#9
Bohdan Andreiuk, Andreas Reisch, Marion Lindecker, Gautier Follain, Nadine Peyriéras, Jacky G Goetz, Andrey S Klymchenko
Fluorescent polymer nanoparticles for long-term labeling and tracking of living cells with any desired color code are developed. They are built from biodegradable poly(lactic-co-glycolic acid) polymer loaded with cyanine dyes (DiO, DiI, and DiD) with the help of bulky fluorinated counterions, which minimize aggregation-caused quenching. At the single particle level, these particles are ≈20-fold brighter than quantum dots of similar color. Due to their identical 40 nm size and surface properties, these nanoparticles are endocytosed equally well by living cells...
August 9, 2017: Small
https://www.readbyqxmd.com/read/28774621/zebrafish-embryo-toxicity-of-15-chlorinated-brominated-and-iodinated-disinfection-by-products
#10
David Hanigan, Lisa Truong, Michael Simonich, Robert Tanguay, Paul Westerhoff
Disinfection to protect human health occurs at drinking water and wastewater facilities through application of non-selective oxidants including chlorine. Oxidants also transform organic material and form disinfection by-products (DBPs), many of which are halogenated and cyto- and genotoxic. Only a handful of assays have been used to compare DBP toxicity, and researchers are unsure which DBP(s) drive the increased cancer risk associated with drinking chlorinated water. The most extensive data set employs an in vitro model cell, Chinese hamster ovary cells...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28738802/histological-and-transcriptomic-effects-of-17%C3%AE-methyltestosterone-on-zebrafish-gonad-development
#11
Stephanie Ling Jie Lee, Julia A Horsfield, Michael A Black, Kim Rutherford, Amanda Fisher, Neil J Gemmell
BACKGROUND: Sex hormones play important roles in teleost ovarian and testicular development. In zebrafish, ovarian differentiation appears to be dictated by an oocyte-derived signal via Cyp19a1a aromatase-mediated estrogen production. Androgens and aromatase inhibitors can induce female-to-male sex reversal, however, the mechanisms underlying gonadal masculinisation are poorly understood. We used histological analyses together with RNA sequencing to characterise zebrafish gonadal transcriptomes and investigate the effects of 17α-methyltestosterone on gonadal differentiation...
July 24, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28666956/coordination-of-cellular-differentiation-polarity-mitosis-and-meiosis-new-findings-from-early-vertebrate-oogenesis
#12
REVIEW
Yaniv M Elkouby, Mary C Mullins
A mechanistic dissection of early oocyte differentiation in vertebrates is key to advancing our knowledge of germline development, reproductive biology, the regulation of meiosis, and all of their associated disorders. Recent advances in the field include breakthroughs in the identification of germline stem cells in Medaka, in the cellular architecture of the germline cyst in mice, in a mechanistic dissection of chromosomal pairing and bouquet formation in meiosis in mice, in tracing oocyte symmetry breaking to the chromosomal bouquet of meiosis in zebrafish, and in the biology of the Balbiani body, a universal oocyte granule...
October 15, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28651189/sex-specific-effects-of-difenoconazole-on-the-growth-hormone-endocrine-axis-in-adult-zebrafish-danio-rerio
#13
Miaomiao Teng, Suzhen Qi, Wentao Zhu, Yao Wang, Dezhen Wang, Yang Yang, Hui Li, Chenglong Li, Kai Dong, Chengju Wang
Difenoconazole, as one of the most widely used triazole fungicides, is applied to protect crops, fruits, and vegetables. It has been reported that difenoconazole can enter the environment and impair aquatic organisms, but whether difenoconazole can disrupt the growth hormone (GH) balance in adult zebrafish (Danio rerio) is still unclear. In this study, adult female and male zebrafish were exposed to difenoconazole (0, 5, 50, and 500µg/L) for seven days. The results revealed that the bioaccumulation of difenoconazole and its primary metabolite difenoconazole alcohol in females were both larger than that in males...
June 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28648994/dynamic-and-differential-expression-of-the-gonadal-aromatase-during-the-process-of-sexual-differentiation-in-a-novel-transgenic-cyp19a1a-egfp-zebrafish-line
#14
Nathalie Hinfray, Frédéric Sohm, Morgane Caulier, Edith Chadili, Benjamin Piccini, Camille Torchy, Jean-Marc Porcher, Yann Guiguen, François Brion
In zebrafish, there exists a clear need for new tools to study sex differentiation dynamic and its perturbation by endocrine disrupting chemicals. In this context, we developed and characterized a novel transgenic zebrafish line expressing green fluorescent protein (GFP) under the control of the zebrafish cyp19a1a (gonadal aromatase) promoter. In most gonochoristic fish species including zebrafish, cyp19a1a, the enzyme responsible for the synthesis of estrogens, has been shown to play a critical role in the processes of reproduction and sexual differentiation...
June 22, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28621416/all-in-one-integrating-cell-polarity-meiosis-mitosis-and-mechanical-forces-in-early-oocyte-differentiation-in-vertebrates
#15
Yaniv M Elkouby
While the differentiation of oocytes is key for embryonic development, and its investigation is crucial for advancing our understanding of human reproduction and fertility, many fundamental questions in oogenesis have been long standing. However, recent technical advances have led to several breakthroughs mainly in mice and zebrafish. Here I review these recent findings, including regulation and organization of the germline cyst, the mechanistics of chromosomal pairing, establishment of cell polarity, and formation of a universal mRNA-protein (mRNP) granule called the Balbiani body...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28601762/warming-modulates-the-effects-of-the-endocrine-disruptor-progestin-levonorgestrel-on-the-zebrafish-fitness-ovary-maturation-kinetics-and-reproduction-success
#16
P G Cardoso, D Rodrigues, T V Madureira, N Oliveira, M J Rocha, E Rocha
Interactive effects between multiple stressors, namely climate drivers (e.g., temperature) and chemical pollution (e.g., endocrine disruptors) are poorly studied. Here, it was for the first time evaluated the combinatory effects of temperature and a synthetic progestin, levonorgestrel (LNG), on the fitness and reproductive-related endpoints of zebrafish (Danio rerio). A multi-factorial design was implemented by manipulating both temperature [setting as baseline an ambient temperature of 27 °C, against warming (+3 °C)] and LNG levels (10 ngL(-1) and 1000 ngL(-1))...
October 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28575219/targeted-disruption-of-aromatase-reveals-dual-functions-of-cyp19a1a-during-sex-differentiation-in-zebrafish
#17
Yike Yin, Haipei Tang, Yun Liu, Yu Chen, Gaofei Li, Xiaochun Liu, Haoran Lin
Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation in fish, but its precise roles during sex differentiation are still largely unknown. Here, we systematically generated cyp19a1a and cyp19a1b mutant lines as well as a cyp19a1a;cyp19a1b double mutant line in zebrafish using transcription activatorlike effector nucleases. Our results showed that cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants, but not cyp19a1b mutants, had impaired sex differentiation, and all cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants were males...
September 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28531178/zebrafish-exposure-to-environmentally-relevant-concentration-of-depleted-uranium-impairs-progeny-development-at-the-molecular-and-histological-levels
#18
Olivier Armant, Kewin Gombeau, Sophia Murat El Houdigui, Magali Floriani, Virginie Camilleri, Isabelle Cavalie, Christelle Adam-Guillermin
Uranium is an actinide naturally found in the environment. Anthropogenic activities lead to the release of increasing amounts of uranium and depleted uranium (DU) in the environment, posing potential risks to aquatic organisms due to radiological and chemical toxicity of this radionucleide. Although environmental contaminations with high levels of uranium have already been observed, chronic exposures of non-human species to levels close to the environmental quality standards remain scarcely characterized. The present study focused on the identification of the molecular pathways impacted by a chronic exposure of zebrafish to 20 μg/L of DU during 10 days...
2017: PloS One
https://www.readbyqxmd.com/read/28400274/characterization-of-gnrh-gnih-elements-in-the-olfacto-retinal-system-and-ovary-during-zebrafish-ovarian-maturation
#19
Sheryll Corchuelo, Emanuel R M Martinez, Arno J Butzge, Lucas B Doretto, Juliana M B Ricci, Fernanda N Valentin, Laura S O Nakaghi, Gustavo M Somoza, Rafael H Nóbrega
Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium...
April 8, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28360383/cloning-and-expression-analysis-of-zygote-arrest-1-zar1-in-new-zealand-white-rabbits
#20
Dan Wang, Shu-Yu Xie, Wei Zhang, Cai-Xia Sun, Tao Huang, An-Si Wang, Xue-Lei Han, Gui-Rong Sun, Ming Li
Zygote arrest 1 (Zar1) is an oocyte-specific maternal-effect gene. Previous studies indicate that Zar1 plays important role in early embryo development, but little is known about its function in rabbit. The objectives of this study were to clone the New Zealand white rabbit Zar1 gene and to investigate its expression in various organs in groups of animals with different reproductive traits.We obtained a 709-bp Zar1 cDNA fragment consisting of an 8-bp exon 1, 161-bp exon 2, 75-bp exon 3, 271-bp exon 4 and 194-bp 3'sequences...
March 2017: Journal of Genetics
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