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https://www.readbyqxmd.com/read/28651189/sex-specific-effects-of-difenoconazole-on-the-growth-hormone-endocrine-axis-in-adult-zebrafish-danio-rerio
#1
Miaomiao Teng, Suzhen Qi, Wentao Zhu, Yao Wang, Dezhen Wang, Yang Yang, Hui Li, Chenglong Li, Kai Dong, Chengju Wang
Difenoconazole, as one of the most widely used triazole fungicides, is applied to protect crops, fruits, and vegetables. It has been reported that difenoconazole can enter the environment and impair aquatic organisms, but whether difenoconazole can disrupt the growth hormone (GH) balance in adult zebrafish (Danio rerio) is still unclear. In this study, adult female and male zebrafish were exposed to difenoconazole (0, 5, 50, and 500µg/L) for seven days. The results revealed that the bioaccumulation of difenoconazole and its primary metabolite difenoconazole alcohol in females were both larger than that in males...
June 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28648994/dynamic-and-differential-expression-of-the-gonadal-aromatase-during-the-process-of-sexual-differentiation-in-a-novel-transgenic-cyp19a1a-egfp-zebrafish-line
#2
Nathalie Hinfray, Frédéric Sohm, Morgane Caulier, Edith Chadili, Benjamin Piccini, Camille Torchy, Jean-Marc Porcher, Yann Guiguen, François Brion
In zebrafish, there exists a clear need for new tools to study sex differentiation dynamic and its perturbation by endocrine disrupting chemicals. In this context, we developed and characterized a novel transgenic zebrafish line expressing green fluorescent protein (GFP) under the control of the zebrafish cyp19a1a (gonadal aromatase) promoter. In most gonochoristic fish species including zebrafish, cyp19a1a, the enzyme responsible for the synthesis of estrogens, has been shown to play a critical role in the processes of reproduction and sexual differentiation...
June 22, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28621416/all-in-one-integrating-cell-polarity-meiosis-mitosis-and-mechanical-forces-in-early-oocyte-differentiation-in-vertebrates
#3
Yaniv M Elkouby
While the differentiation of oocytes is key for embryonic development, and its investigation is crucial for advancing our understanding of human reproduction and fertility, many fundamental questions in oogenesis have been long standing. However, recent technical advances have led to several breakthroughs mainly in mice and zebrafish. Here I review these recent findings, including regulation and organization of the germline cyst, the mechanistics of chromosomal pairing, establishment of cell polarity, and formation of a universal mRNA-protein (mRNP) granule called the Balbiani body...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28601762/warming-modulates-the-effects-of-the-endocrine-disruptor-progestin-levonorgestrel-on-the-zebrafish-fitness-ovary-maturation-kinetics-and-reproduction-success
#4
P G Cardoso, D Rodrigues, T V Madureira, N Oliveira, M J Rocha, E Rocha
Interactive effects between multiple stressors, namely climate drivers (e.g., temperature) and chemical pollution (e.g., endocrine disruptors) are poorly studied. Here, it was for the first time evaluated the combinatory effects of temperature and a synthetic progestin, levonorgestrel (LNG), on the fitness and reproductive-related endpoints of zebrafish (Danio rerio). A multi-factorial design was implemented by manipulating both temperature [setting as baseline an ambient temperature of 27 °C, against warming (+3 °C)] and LNG levels (10 ngL(-1) and 1000 ngL(-1))...
June 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28575219/targeted-disruption-of-aromatase-reveals-dual-functions-of-cyp19a1a-during-sex-differentiation-in-zebrafish
#5
Yike Yin, Haipei Tang, Yun Liu, Yu Chen, Gaofei Li, Xiaochun Liu, Haoran Lin
Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation in fish, but its precise roles during sex differentiation are still largely unknown. Here, we systematically generated cyp19a1a and cyp19a1b mutant lines as well as a cyp19a1a;cyp19a1b double mutant line in zebrafish using transcription activator-like effectors nucleases (TALENs). Our results showed that cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants, but not cyp19a1b mutants, have impaired sex differentiation, and all cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants are males...
May 29, 2017: Endocrinology
https://www.readbyqxmd.com/read/28531178/zebrafish-exposure-to-environmentally-relevant-concentration-of-depleted-uranium-impairs-progeny-development-at-the-molecular-and-histological-levels
#6
Olivier Armant, Kewin Gombeau, Sophia Murat El Houdigui, Magali Floriani, Virginie Camilleri, Isabelle Cavalie, Christelle Adam-Guillermin
Uranium is an actinide naturally found in the environment. Anthropogenic activities lead to the release of increasing amounts of uranium and depleted uranium (DU) in the environment, posing potential risks to aquatic organisms due to radiological and chemical toxicity of this radionucleide. Although environmental contaminations with high levels of uranium have already been observed, chronic exposures of non-human species to levels close to the environmental quality standards remain scarcely characterized. The present study focused on the identification of the molecular pathways impacted by a chronic exposure of zebrafish to 20 μg/L of DU during 10 days...
2017: PloS One
https://www.readbyqxmd.com/read/28400274/characterization-of-gnrh-gnih-elements-in-the-olfacto-retinal-system-and-ovary-during-zebrafish-ovarian-maturation
#7
Sheryll Corchuelo, Emanuel R M Martinez, Arno J Butzge, Lucas B Doretto, Juliana M B Ricci, Fernanda N Valentin, Laura S O Nakaghi, Gustavo M Somoza, Rafael H Nóbrega
Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium...
April 8, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28360383/cloning-and-expression-analysis-of-zygote-arrest-1-zar1-in-new-zealand-white-rabbits
#8
Dan Wang, Shu-Yu Xie, Wei Zhang, Cai-Xia Sun, Tao Huang, An-Si Wang, Xue-Lei Han, Gui-Rong Sun, Ming Li
Zygote arrest 1 (Zar1) is an oocyte-specific maternal-effect gene. Previous studies indicate that Zar1 plays important role in early embryo development, but little is known about its function in rabbit. The objectives of this study were to clone the New Zealand white rabbit Zar1 gene and to investigate its expression in various organs in groups of animals with different reproductive traits.We obtained a 709-bp Zar1 cDNA fragment consisting of an 8-bp exon 1, 161-bp exon 2, 75-bp exon 3, 271-bp exon 4 and 194-bp 3'sequences...
March 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28342328/reproductive-toxicity-of-%C3%AE-diketone-antibiotic-mixtures-to-zebrafish-danio-rerio
#9
Xuedong Wang, Yan Ma, Jinfeng Liu, Xiaohan Yin, Zhiheng Zhang, Caihong Wang, Yanyan Li, Huili Wang
So far, few data are available on the reproductive toxicological assessment of β-diketone antibiotics (DKAs), a class of ubiquitous pseudo-persistent pollutant, in zebrafish (Danio rerio). Herein, we reported the reproductive effects of DKAs by means of transcriptome analysis (F1-zebrafish), changes in a series of reproductive indices (F0-zebrafish) and histopathological observations. A total of 1170, 983 and 1399 genes were found to be differentially expressed when compared control vs. 6.25mg/L, control vs...
March 22, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28324048/demonstration-of-a-functional-kiss-kissr-system-in-amphioxus-with-implications-for-origin-of-neuroendocrine-regulation
#10
Peng Wang, Meng Wang, Guangdong Ji, Shuangshuang Yang, Shicui Zhang, Zhenhui Liu
Amphioxus belongs to the Cephalochordata, which is the most basal subphylum of the chordates. Despite many studies on the endocrine system of amphioxus, key information about its regulation remains ambiguous. Here we clearly demonstrated the presence of a functional kisspeptin/kisspeptin receptor (Kiss-Kissr) system, which is involved in the regulation of reproduction in amphioxus. Evolutionary analyses revealed large expansion of Kiss and Kissr (gpr54) genes in amphioxus and they might represent the ancestral type of the Kiss/gpr54 genes in chordates...
January 24, 2017: Endocrinology
https://www.readbyqxmd.com/read/28317950/in-vivo-identification-of-potential-uranium-protein-targets-in-zebrafish-ovaries-after-chronic-waterborne-exposure
#11
Y Eb-Levadoux, S Frelon, O Simon, C Arnaudguilhem, R Lobinski, S Mounicou
Ecotoxicological studies have indicated the reprotoxicity of uranium (U) in zebrafish, but its molecular mechanisms remain unclear. Due to the non-covalent nature of U-protein complexes, canonical proteomics approaches are often not relevant as they usually use denaturating reagents or solvents. In this study, non-denaturating (ND) methods were used to obtain insight into the nature of U potential targets in ovaries of reproduced and non-reproduced zebrafish after 20 days of exposure to an environmentally relevant U concentration (20 μg L(-1))...
May 24, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28295807/interaction-of-melatonin-and-gonadotropin-inhibitory-hormone-on-the-zebrafish-brain-pituitary-reproductive-axis
#12
Thangal Yumnamcha, Zeeshan A Khan, Chongtham Rajiv, Sijagurumayum D Devi, Gopinath Mondal, Haobijam Sanjita Devi, Rupjyoti Bharali, Asamanja Chattoraj
Circadian cycles and photoperiod are known to influence reproductive physiology in several animals. Neuropeptides, such as gonadotropin-inhibitory hormone (GNIH) and gonadotropin-releasing hormone (GNRH), are influenced by melatonin in birds and mammals. The present study demonstrates the role of melatonin in oocyte maturation in the zebrafish (Danio rerio), via the brain-pituitary-reproductive axis, under different photic conditions. Melatonin was significantly higher both in the whole brain and ovary under continuous dark (DD) compared to continuous light (LL) conditions...
May 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28281645/dna-interference-mediated-screening-of-maternal-factors-in-the-chordate-oikopleura-dioica
#13
Tatsuya Omotezako, Masaki Matsuo, Takeshi A Onuma, Hiroki Nishida
The maternal contribution to the oocyte cytoplasm plays an important role during embryogenesis because it is involved in early cell fate specification and embryonic axis establishment. However, screening projects targeting maternal factors have only been conducted in a limited number of animal models, such as nematodes, fruit flies, and zebrafish, while few maternal genes have been analysed because of difficulties encountered in inhibiting gene products already expressed in the ovaries. Therefore, simple and efficient methods for large-scale maternal screening are necessary...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28262836/dynamics-of-mirna-transcriptome-during-gonadal-development-of-zebrafish
#14
Christopher Presslauer, Teshome Tilahun Bizuayehu, Martina Kopp, Jorge M O Fernandes, Igor Babiak
Studies in non-teleost vertebrates have found microRNAs (miRNAs) to be essential for proper gonadal development. However, comparatively little is known about their role during gonadal development in teleost fishes. So far in zebrafish, a model teleost, transcript profiling throughout gonadal development has not been established because of a tiny size of an organ in juvenile stages and its poor distinguishability from surrounding tissues. We performed small RNA sequencing on isolated gonads of See-Thru-Gonad line, from the undifferentiated state at 3 weeks post fertilization (wpf) to fully mature adults at 24 wpf...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28254490/lh-signaling-induced-ptgs2a-expression-is-required-for-ovulation-in-zebrafish
#15
Haipei Tang, Yun Liu, Jianzhen Li, Gaofei Li, Yu Chen, Yike Yin, Yin Guo, Christopher H K Cheng, Xiaochun Liu, Haoran Lin
It is well known that ovulation is induced by luteinizing hormone (LH) surge. However, the down-stream factors that mediating LH surge induced ovulation are less clear. The cyclooxygenases (also known as PTGS) as key enzymes for prostaglandins synthesis appear to be important for ovulation in mammals, but their functional roles and molecular mechanism in regulation of fish ovulation are largely unexplored. In this study, we have systematically investigated the expression, regulation and functional roles of cox genes during zebrafish ovulation...
February 28, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28193729/sequential-divergent-and-cooperative-requirements-of-foxl2a-and-foxl2b-in-ovary-development-and-maintenance-of-zebrafish
#16
Yan-Jing Yang, Yang Wang, Zhi Li, Li Zhou, Jian-Fang Gui
Foxl2 is essential for mammalian ovary maintenance. Although sexually dimorphic expression of foxl2 was observed in many teleosts, its role and regulative mechanism in fish remained largely unclear. In this study, we first identified two transcript variants of foxl2a and its homologous gene foxl2b in zebrafish, and revealed their specific expression in follicular layer cells in a sequential and divergent fashion during ovary differentiation, maturation, and maintenance. Then, homozygous foxl2a mutants (foxl2a(-/-)) and foxl2b mutants (foxl2b(-/-)) were constructed and detailed comparisons, such as sex ratio, gonadal histological structure, transcriptome profiling, and dynamic expression of gonadal development-related genes, were carried out...
April 2017: Genetics
https://www.readbyqxmd.com/read/28118999/reproductive-and-transcriptional-effects-of-the-antiandrogenic-progestin-chlormadinone-acetate-in-zebrafish-danio-rerio
#17
Patricia Franziska Siegenthaler, Yanbin Zhao, Kun Zhang, Karl Fent
Chlormadinone acetate (CMA) is a frequently used progestin with antiandrogenic activity in humans. Residues may enter the aquatic environment but potential adverse effects in fish are unknown. While our previous work focused on effects of CMA in vitro and in zebrafish eleuthero-embryos, the present study reports on reproductive and transcriptional effects in adult female and male zebrafish (Danio rerio). We performed a reproductive study using breeding groups of zebrafish. After 15 days of pre-exposure, we exposed zebrafish to different measured concentrations between 6...
April 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28013034/spatiotemporal-expression-analysis-of-nuclear-estrogen-receptors-in-the-zebrafish-ovary-and-their-regulation-in-vitro-by-endocrine-hormones-and-paracrine-factors
#18
Ka-Cheuk Liu, Shuk-Wa Lau, Wei Ge
Estradiol (E2) stimulates luteinizing hormone receptor (lhcgr) expression via nuclear estrogen receptors (nERs) in the zebrafish ovary. We have demonstrated that endocrine hormones such as gonadotropin (hCG) and paracrine factors such as epidermal growth factor (EGF) and pituitary adenylate cyclase-activating peptide (PACAP) can modulate E2-induced lhcgr expression in vitro. These observations raised a question on whether these hormones and factors exert their effects via regulating the expression of nERs. In this study, we first characterized the spatiotemporal expression profiles of three nER subtypes in the zebrafish ovary, including esr1 (ERα), esr2a (ERβ2) and esr2b (ERβ1)...
May 15, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28002497/a-novel-igf3-gene-in-common-carp-cyprinus-carpio-evidence-for-its-role-in-regulating-gonadal-development
#19
Feibiao Song, Lanmei Wang, Wenbin Zhu, Jianjun Fu, Juanjuan Dong, Zaijie Dong
Since the insulin-like growth factor 3 (igf3) gene was recently discovered in fish ovary, its function in the gonads has received much attention. In this study, we isolated two igf3 subtypes from common carp (Cyprinus carpio), which comprised full-length cDNA of 707 and 1153 nucleotides encoding 205 and 198 amino acids (aa), respectively. The Igf3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish (Danio rerio). Phylogenetic tree construction revealed that the C. carpio igf3 gene was first clustered with D...
2016: PloS One
https://www.readbyqxmd.com/read/27988227/methods-for-the-analysis-of-early-oogenesis-in-zebrafish
#20
Yaniv M Elkouby, Mary C Mullins
Oocyte differentiation is a highly dynamic and intricate developmental process whose mechanistic understanding advances female reproduction, fertility, and ovarian cancer biology. Despite the many attributes of the zebrafish model, it has yet to be fully exploited for the investigation of early oocyte differentiation and ovarian development. This is partly because the properties of the adult zebrafish ovary make it technically challenging to access early stage oocytes. As a result, characterization of these stages has been lacking and tools for their analysis have been insufficient...
December 14, 2016: Developmental Biology
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