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https://www.readbyqxmd.com/read/29775928/tributyltin-impaired-reproductive-success-in-female-zebrafish-through-disrupting-oogenesis-reproductive-behaviors-and-serotonin-synthesis
#1
Wei-Yang Xiao, Ying-Wen Li, Qi-Liang Chen, Zhi-Hao Liu
Tributyltin (TBT), an organotin acting as aromatase (Cyp19a1) inhibitor, has been found to disrupt gametogenesis and reproductive behaviors in several fish species. However, few studies addressing the mechanisms underlying the impaired gametogenesis and reproduction have been reported. In this study, female adults of zebrafish (Danio rerio) were continuously exposed to two nominal concentrations of TBT (100 and 500 ng/L, actual concentrations: 90.8 ± 1.3 ng/L and 470.3 ± 2.7 ng/L, respectively) for 28 days...
May 12, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29758382/effects-of-exposure-to-bpf-on-development-and-sexual-differentiation-during-early-life-stages-of-zebrafish-danio-rerio
#2
Qian Yang, Xianhai Yang, Jining Liu, Yingwen Chen, Shubao Shen
Bisphenol F (BPF) has become a predominant bisphenol contaminant in recent years. It has significant estrogenic properties in both in vivo and in vitro studies. We have previously studied the disrupting mechanisms of BPF on the hypothalamic-pituitary-gonadal axis of adult zebrafish. However, the effects of BPF exposure on development and sexual differentiation of zebrafish embryos/larvae remain unclear. To determine the effects of BPF on the critical stage of sex differentiation in zebrafish, zebrafish embryos/larvae were exposed to 1, 10, 100, and 1000 μg/L BPF from fertilization to 60 days post-fertilization (dpf)...
May 11, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29753248/embryonic-exposure-to-benzo-a-pyrene-inhibits-reproductive-capability-in-adult-female-zebrafish-and-correlation-with-dna-methylation
#3
Dongxu Gao, Jing Lin, Kunlin Ou, Ying Chen, Hongbin Li, Qinhua Dai, Zhenni Yu, Zhenghong Zuo, Chonggang Wang
This study was conducted to investigate the effects of embryonic short-term exposure to benzo(a)pyrene (BaP), a model polycyclic aromatic hydrocarbon, on ovarian development and reproductive capability in adult female zebrafish. In 1-year-old fish after embryonic exposure to BaP for 96 h, the gonadosomatic indices and the percentage of mature oocytes were significantly decreased in the 0.5, 5 and 50 nmol/L treatments. The spawned egg number, the fertilization rate and the hatching success were significantly reduced, while the malformation rate of the F1 unexposed larvae were increased...
May 9, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29735715/znrf3-functions-in-mammalian-sex-determination-by-inhibiting-canonical-wnt-signaling
#4
Abigail Harris, Pam Siggers, Silvia Corrochano, Nick Warr, Danielle Sagar, Daniel T Grimes, Makoto Suzuki, Rebecca D Burdine, Feng Cong, Bon-Kyoung Koo, Hans Clevers, Isabelle Stévant, Serge Nef, Sara Wells, Raja Brauner, Bochra Ben Rhouma, Neïla Belguith, Caroline Eozenou, Joelle Bignon-Topalovic, Anu Bashamboo, Ken McElreavey, Andy Greenfield
Mammalian sex determination is controlled by the antagonistic interactions of two genetic pathways: The SRY-SOX9-FGF9 network promotes testis determination partly by opposing proovarian pathways, while RSPO1/WNT-β-catenin/FOXL2 signals control ovary development by inhibiting SRY-SOX9-FGF9. The molecular basis of this mutual antagonism is unclear. Here we show that ZNRF3, a WNT signaling antagonist and direct target of RSPO1-mediated inhibition, is required for sex determination in mice. XY mice lacking ZNRF3 exhibit complete or partial gonadal sex reversal, or related defects...
May 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29655816/sex-specific-transcription-and-dna-methylation-profiles-of-reproductive-and-epigenetic-associated-genes-in-the-gonads-and-livers-of-breeding-zebrafish
#5
L V Laing, J Viana, E Dempster, T M Uren Webster, R van Aerle, J Mill, E M Santos
Reproduction is an essential process for life and is regulated by complex hormone networks and environmental factors. To date, little is known about the contribution of epigenetic mechanisms to the regulation of reproduction, particularly in lower vertebrates. We used the zebrafish (Danio rerio) model to investigate the sex-specific transcription and DNA methylation profiles for genes involved in the regulation of reproduction and in epigenetic signalling in the livers and gonads. We found evidence for associations between DNA promotor methylation and transcription for esr1 (gonads and female livers), amh (gonads) and dnmt1 (livers)...
April 12, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29627575/the-dual-role-of-cgmp-in-oocyte-maturation-of-zebrafish
#6
Jianzhen Li, Wenni Zhou, Yamei Wang, Caiyan Niu
The roles of cyclic guanosine monophosphate (cGMP) signaling in oocyte maturation attracts much attention in mammals, but its roles in fish are still largely unknown. Using zebrafish as a model, we demonstrated for the first time in fish that cGMP is involved in oocyte maturation, and its functional model in oocyte maturation is different from that of mammals. The intracellular cGMP could be regulated by nitric oxide (NO), we found that all three NO synthase enzymes and four soluble guanylyl cyclases (sGC) are expressed in the zebrafish ovary...
April 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29574323/deep-insights-into-the-mode-of-atp-binding-mechanism-in-zebrafish-cyclin-dependent-protein-kinase-like-1-zcdkl1-a-molecular-dynamics-approach
#7
Ajaya Kumar Rout, Budheswar Dehury, Jitendra Maharana, Chirasmita Nayak, Vishwamitra Singh Baisvar, Bijay Kumar Behera, Basanta Kumar Das
In eukaryotes, the serine/threonine kinases (STKs) belonging to cyclin-dependent protein kinases (CDKs) play significant role in control of cell division and curb transcription in response to several extra and intra-cellular signals indispensable for enzymatic activity. The zebrafish cyclin-dependent protein kinase-like 1 protein (zCDKL1) shares a high degree of sequence and structural similarity with mammalian orthologs and express in brain, ovary, testis, and low levels in other tissues. Regardless of its importance in the developmental process, the structure, function and mode of ATP recognition have not been investigated yet due to lack of experimental data...
February 13, 2018: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29507535/high-sensitivity-and-high-resolution-in-situ-hybridization-of-coding-and-long-non-coding-rnas-in-vertebrate-ovaries-and-testes
#8
Natsumi Takei, Takuma Nakamura, Shohei Kawamura, Yuki Takada, Yui Satoh, Atsushi P Kimura, Tomoya Kotani
Background: Subcellular localization of coding and non-coding RNAs has emerged as major regulatory mechanisms of gene expression in various cell types and many organisms. However, techniques that enable detection of the subcellular distribution of these RNAs with high sensitivity and high resolution remain limited, particularly in vertebrate adult tissues and organs. In this study, we examined the expression and localization of mRNAs encoding Pou5f1/Oct4, Mos, Cyclin B1 and Deleted in Azoospermia-like (Dazl) in zebrafish and mouse ovaries by combining tyramide signal amplification (TSA)-based in situ hybridization with paraffin sections which can preserve cell morphology of tissues and organs at subcellular levels...
2018: Biological Procedures Online
https://www.readbyqxmd.com/read/29494622/ahr2-required-for-normal-behavioral-responses-and-proper-development-of-the-skeletal-and-reproductive-systems-in-zebrafish
#9
Gloria R Garcia, Sean M Bugel, Lisa Truong, Sean Spagnoli, Robert L Tanguay
The aryl hydrocarbon receptor (AHR) is a conserved ligand-activated transcription factor required for proper vertebrate development and homeostasis. The inappropriate activation of AHR by ubiquitous pollutants can lead to adverse effects on wildlife and human health. The zebrafish is a powerful model system that provides a vertebrate data stream that anchors hypothesis at the genetic and cellular levels to observations at the morphological and behavioral level, in a high-throughput format. In order to investigate the endogenous functions of AHR, we generated an AHR2 (homolog of human AHR)-null zebrafish line (ahr2osu1) using the clustered, regulatory interspaced, short palindromic repeats (CRISPR)-Cas9 precision genome editing method...
2018: PloS One
https://www.readbyqxmd.com/read/29425915/toxicokinetic-and-toxicodynamic-of-depleted-uranium-in-the-zebrafish-danio-rerio
#10
Olivier Simon, Béatrice Gagnaire, Virginie Camilleri, Isabelle Cavalié, Magali Floriani, Christelle Adam-Guillermin
This study investigated the accumulation pattern and biological effects (genotoxicity and histopathology) to adult zebrafish (male and female) exposed to a nominal waterborne concentration of 20 μg L-1 of depleted uranium (DU) for 28 days followed by 27 days of depuration. Accumulation pattern showed that (i) DU accumulated in brain, (ii) levels in digestive tract were higher than those measured in gills and (iii) levels remained high in kidney, brain and ovary despite the 27 days of depuration period...
April 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29409886/changes-in-the-morphology-and-gene-expression-of-developing-zebrafish-gonads
#11
REVIEW
Chen-Wei Hsu, You-Jiun Pan, Yan-Wei Wang, Sok-Keng Tong, Bon-Chu Chung
Zebrafish gonadal sexual differentiation is an important but poorly understood subject. The difficulty in investigating zebrafish sexual development lies in its sex determination plasticity, the lack of morphological tools to distinguish juvenile females from males, and the lack of sex chromosomes in laboratory strains. Zebrafish sexual differentiation starts at around 8 days post-fertilization when germ cells start to proliferate. The number of germ cells determines the future sex of the gonad. Gonads with more germ cells differentiate into ovaries, whereas a reduced germ cell number leads to male-biased sexual differentiation...
January 31, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29307115/bioinformatic-analyses-of-zona-pellucida-genes-in-vertebrates-and-their-expression-in-nile-tilapia
#12
Tianli Wu, Yunying Cheng, Zhilong Liu, Wenjing Tao, Shuqing Zheng, Deshou Wang
Zona pellucida (ZP) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4 ZP genes belonging to 5 subfamilies (ZPA, ZPB, ZPC, ZPD, and ZPAX) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively...
April 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29258955/evaluation-of-toxicological-endpoints-in-female-zebrafish-after-bisphenol-a-exposure
#13
Ana M Molina, Nieves Abril, Noelia Morales-Prieto, José G Monterde, Antonio J Lora, Nahúm Ayala, Rosario Moyano
Given the importance of bisphenol A (BPA) as a xenoestrogen and its potential effects on human and animal health, we evaluated BPA exposure's short-term effects on follicular development, yolk protein vitellogenin (VTG) production and aromatase expression in female zebrafish. Histological modifications were observed along with increased presence of atretic follicles. Whole-body VTG concentration increased with the dose of BPA exposure. In contrast, expression of Cyp19a mRNA in the ovaries of BPA-exposed fish exhibited an apparent non-monotonic response curve, marked by downregulation at 1 μg/L BPA, upregulation at 10 μg/L BPA, and a return to downregulation at 100 μg/L BPA and higher doses...
February 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29237725/abin-1-negatively-regulates-%C3%AE-opioid-receptor-function
#14
Peilan Zhou, Jiebing Jiang, Hui Yan, Yulei Li, Junru Zhao, Xiao Wang, Ruibin Su, Zehui Gong
The μ -opioid receptor (MOR) is a Gi/o protein-coupled receptor that mediates analgesic, euphoric, and reward effects. Using a bacterial two-hybrid screen, we reported that the carboxyl tail of the rat MOR associates with A20-binding inhibitor of nuclear factor κ B (ABIN-1). This interaction was confirmed by direct protein-protein binding and coimmunoprecipitation of MOR and ABIN-1 proteins in cell lysates. Saturation binding studies showed that ABIN-1 had no effect on MOR binding. However, the interaction of ABIN-1 and MOR inhibited the activation of G proteins induced by DAMGO ([d-Ala2,N-Me-Phe4,Gly5-ol]-Enkephalin)...
February 2018: Molecular Pharmacology
https://www.readbyqxmd.com/read/29229882/igf1-stimulates-differentiation-of-primary-follicles-and-their-growth-in-ovarian-explants-of-zebrafish-danio-rerio-cultured-in-vitro
#15
Pancharatna A Katti, Sheetal S Narvekar, Basavaraj B Goundadkar, Prasad A Deshpande
The present study is an attempt to elucidate the involvement of insulin-like growth factor (IGF1) in the differentiation and growth of primary follicles in ovarian explant cultures of zebrafish. Ovaries from adult females were cultured in triplicate sets/treatment group for 15 days at 22° in the laboratory. Culture medium was supplemented with either insulin (1 ng/mL) or IGF1 (1 ng/mL) or insulin + IGF1 (Experiment 1) or 0.1 or 1.0 or 10 ng/mL of IGF1 (Experiment 2). Ovaries cultured in medium alone served as controls and those fixed at the beginning of the culture as initial controls...
December 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/29228146/identification-and-characterization-of-a-specific-13-mirna-expression-signature-during-follicle-activation-in-the-zebrafish-ovary
#16
Queenie Wing-Lei Wong, Ming-An Sun, Shuk-Wa Lau, Chirag Parsania, Shaolong Zhou, Silin Zhong, Wei Ge
Ovarian folliculogenesis is always of great interest in reproductive biology. However, the molecular mechanisms that control follicle development, particularly the early phase of follicle activation or recruitment, still remain poorly understood. In an attempt to decipher the gene networks and signaling pathways involved in such transition, we conducted a transcriptomic analysis (RNA-seq) on zebrafish primary growth (PG, stage I; inactive) and previtellogenic (PV, stage II; activated) follicles. A total of 118 unique microRNAs (miRNAs) (11 downregulated and 83 upregulated during PG/PV transition) and 56711 unique messenger RNAs (mRNAs) (1839 downregulated and 7243 upregulated during PG/PV transition) were identified...
January 1, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29183794/characterization-of-carp-gonadotropins-structure-annual-profile-and-carp-and-zebrafish-pituitary-topographic-organization
#17
Lian Hollander-Cohen, Matan Golan, Joseph Aizen, Michal Shpilman, Berta Levavi-Sivan
Two gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), are important players in the hypothalamic-pituitary-gonadal axis of vertebrates. In the present work, we describe the construction of recombinant (r) common carp (Cyprinus carpio; c) FSH (rcFSH) and LH (rcLH) using the Pichia pastoris system, the generation of specific antibodies against their respective β subunits, and their use in the development and validation of specific ELISAs. We produced carp rLH and rFSH as single-chain polypeptides, wherein the GTH subunit α was joined with either cLHβ or cFSHβ mature protein-coding sequences to form a fusion gene that encodes a yoked polypeptide, in which the GTH β-subunit forms the N-terminal part and the α-subunit forms the C-terminal part...
November 26, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29173213/evaluation-of-reference-genes-for-reverse-transcription-quantitative-pcr-assays-in-organs-of-zebrafish-exposed-to-glyphosate-based-herbicide-roundup
#18
M L Jaramillo, A G Pereira, C E Davico, L Nezzi, D Ammar, Y M R Müller, E M Nazari
Roundup is a glyphosate-based herbicide (GBH) widely used in agriculture and may cause toxic effects in non-target organisms. Model organisms, as zebrafish, and analysis of gene expression by reverse transcription-quantitative PCR (RT-qPCR) could be used to better understand the Roundup toxicity. A prerequisite for RT-qPCR is the availability of appropriate reference genes; however, they have not been described for Roundup-exposed fish. The aim of this study was to evaluate the expression stability of six reference genes (rpl8, β-act, gapdh, b2m, ef1α, hprt1) and one expressed repetitive element (hatn10) in organs of males (brain, gill, testis) and females (ovary) of zebrafish exposed to Roundup WG at three concentrations (0...
November 27, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29096293/effects-of-the-bioconcentration-and-parental-transfer-of-environmentally-relevant-concentrations-of-difenoconazole-on-endocrine-disruption-in-zebrafish-danio-rerio
#19
Miaomiao Teng, Suzhen Qi, Wentao Zhu, Yao Wang, Dezhen Wang, Kai Dong, Chengju Wang
Difenoconazole, a typical triazole fungicide, inhibits lanosterol-14R-demethylase (CYP51) to prevent fungal sterol synthesis and its residues are frequently detected in the environment due to its wide application. Previous studies have demonstrated that difenoconazole altered the triglyceride levels, and gene expression relevant to cholesterol biosynthesis in zebrafish. However, endocrine-disruption in the hypothalamus-pituitary-gonadal-liver (HPGL) axis, the effects of transferring to offspring, and the underlying mechanisms of difenoconazole in aquatic organisms are still unknown...
February 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29055862/juvenile-exposure-to-bisphenol-a-promotes-ovarian-differentiation-but-suppresses-its-growth-potential-involvement-of-pituitary-follicle-stimulating-hormone
#20
Weiting Chen, Shuk-Wa Lau, Yuqin Fan, Rudolf S S Wu, Wei Ge
Bisphenol A (BPA), a plastic monomer and plasticizer, is commonly used in plastics industry, and it has been well documented to be an estrogenic endocrine disrupter. In the present study, we investigated the effect of early (juvenile) exposure to BPA on the hypothalamic-pituitary-gonad (HPG) axis in the zebrafish. Estradiol (E2) and testosterone (T) were also included as positive and negative controls respectively. Juvenile zebrafish were exposed to BPA (1 and 10μM), E2 (10nM) and T (10nM) from 20 to 40 dpf (days post-fertilization), the period of sex/gonadal differentiation, followed by histological and expression analyses at 40 dpf...
December 2017: Aquatic Toxicology
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