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https://www.readbyqxmd.com/read/28738536/effect-of-apigenin-kaempferol-and-resveratrol-on-the-gene-expression-and-protein-secretion-of-tumor-necrosis-factor-alpha-tnf-%C3%AE-and-interleukin-10-il-10-in-raw-264-7-macrophages
#1
Marta Palacz-Wrobel, Paulina Borkowska, Monika Paul-Samojedny, Malgorzata Kowalczyk, Anna Fila-Danilow, Renata Suchanek-Raif, Jan Kowalski
Polyphenols such as apigenin, kaempferol or resveratrol are typically found in plants, including fruits, vegetables, herbs and spices, which have a wide range of biological functions such as antioxidative, anti-inflammatory, vasodilative, anticoagulative and proapoptotic. Discovering such multifunctional compounds in widely consumed plant-based products - ones that both inhibit the release of TNF-α from tissue macrophages and at the same time enhance the secretion of IL-10 - would be an important signpost in the quest for effective pharmacological treatment of numerous diseases that have an inflammatory etiology...
July 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738524/elevated-microrna-125b-promotes-inflammation-in-rheumatoid-arthritis-by-activation-of-nf-%C3%AE%C2%BAb-pathway
#2
Bo Zhang, Li-Song Wang, Yu-Hu Zhou
microRNA-125b (miR-125b) has been reported to be increased in rheumatoid arthritis (RA). However, the role of miR-125b in RA remains to be fully elucidated. We aimed to explore the functional role of miR-125b in RA, as well as the underlying mechanism. The expression of miR-125b in serum and synovial tissues of patients with RA and healthy controls was confirmed. In addition, the levels of miR-125b in lipopolysaccharide (LPS)-stimulated fibroblast-like synoviocytes (FLS) were also measured. FLS were transfected with miR-125b mimic, antisense oligonucleotides (ASO)-miR-125b, pcDNA-inhibitor of NF-κB (IκB)-α or corresponding controls, and then stimulated with LPS or incubated with nuclear factor kappa B (NF-κB) inhibitors pyrrolidine dithiocarbamate (PDTC)...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738499/aqueous-extract-of-codium-fragile-suppressed-inflammatory-responses-in-lipopolysaccharide-stimulated-raw264-7-cells-and-carrageenan-induced-rats
#3
Seul Ah Lee, Sung-Min Moon, Yun Hee Choi, Seul Hee Han, Bo-Ram Park, Mi Suk Choi, Jae-Sung Kim, Yong Hwan Kim, Do Kyung Kim, Chun Sung Kim
Codium fragile (Suringar) Hariot has been used in Oriental medicine for the treatment of enterobiasis, dropsy, and dysuria and has been shown to have various biological effects. In this study, we evaluated the anti-inflammatory effects of aqueous extract of C. fragile (AECF) using in vitro and in vivo models. Nitric oxide (NO), prostaglandin E2 (PGE2), inflammatory-related mRNAs, and proteins were determined using the Griess assay, enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR), and western blotting, respectively...
July 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738497/oeanolic-acid-protects-against-the-hepatotoxicity-of-d-galactosame-plus-endotoxin-in-mice
#4
Xiao-Li Wan, Yuan-Fu Lu, Shang-Fu Xu, Qin Wu, Jie Liu
Oleanolic acid (OA) is a triterpenoid contained in many herbal medicines. The aim of this study was to investigate the protective effect of OA against D-galactosame plus lipopolysaccharides (D-GalN/LPS)-induced acute liver injury and the underling mechanisms. Mice were randomly divided into normal control with vehicles only (corn oil), D-GalN/LPS only (700mg/10μg/kg, ip), OA-po (200μmol/kg in corn oil, po) plus D-GalN/LPS, and OA-sc (50μmol/kg in 2% tween 80, sc) plus D-GalN/LPS groups. OA pretreatment was conducted twice daily for 4 consecutive days...
July 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738247/asiatic-acid-inhibits-lps-induced-inflammatory-response-in-human-gingival-fibroblasts
#5
Chunbo Hao, Buling Wu, Zhiming Hou, Qi Xie, Tianan Liao, Tao Wang, Dandan Ma
Asiatic acid, a triterpenoid component isolated from Centella asiatica (L.) Urban, possesses antioxidative and anti-inflammatory activities. In this study, we aimed to investigate the anti-inflammatory effects of asiatic acid both in vivo and in vitro. HGFs or RAW264.7 cells were treated with asiatic acid 1h before LPS treatment. Cell viability was measured by MTT assay. The levels of PGE2, NO, IL-6, and IL-8 were detected by ELISA. Protein expression levels were detected by western blot analysis. In vivo, asiatic acid significantly inhibited LPS-induced IL-6 and IL-8 expression levels in gingival tissues...
July 21, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28737669/molecular-dynamics-simulations-of-the-host-defense-peptide-temporin-l-and-its-q3k-derivative-an-atomic-level-view-from-aggregation-in-water-to-bilayer-perturbation
#6
Andrea Farrotti, Paolo Conflitti, Saurabh Srivastava, Jimut Kanti Ghosh, Antonio Palleschi, Lorenzo Stella, Gianfranco Bocchinfuso
Temporin L (TempL) is a 13 residue Host Defense Peptide (HDP) isolated from the skin of frogs. It has a strong affinity for lipopolysaccharides (LPS), which is related to its high activity against Gram-negative bacteria and also to its strong tendency to neutralize the pro-inflammatory response caused by LPS release from inactivated bacteria. A designed analog with the Q3K substitution shows an enhancement in both these activities. In the present paper, Molecular Dynamics (MD) simulations have been used to investigate the origin of these improved properties...
July 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28737175/natural-variation-of-macrophage-activation-as-disease-relevant-phenotype-predictive-of-inflammation-and-cancer-survival
#7
Konrad Buscher, Erik Ehinger, Pritha Gupta, Akula Bala Pramod, Dennis Wolf, George Tweet, Calvin Pan, Charles D Mills, Aldons J Lusis, Klaus Ley
Although mouse models exist for many immune-based diseases, the clinical translation remains challenging. Most basic and translational studies utilize only a single inbred mouse strain. However, basal and diseased immune states in humans show vast inter-individual variability. Here, focusing on macrophage responses to lipopolysaccharide (LPS), we use the hybrid mouse diversity panel (HMDP) of 83 inbred strains as a surrogate for human natural immune variation. Since conventional bioinformatics fail to analyse a population spectrum, we highlight how gene signatures for LPS responsiveness can be derived based on an Interleukin-12β and arginase expression ratio...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28736552/asiatic-acid-exhibits-anti-inflammatory-and-antioxidant-activities-against-lipopolysaccharide-and-d-galactosamine-induced-fulminant-hepatic-failure
#8
Hongming Lv, Zhimin Qi, Sisi Wang, Haihua Feng, Xuming Deng, Xinxin Ci
Inflammation and oxidative stress are essential for the pathogenesis of fulminant hepatic failure (FHF). Asiatic acid (AA), which is a pentacyclic triterpene that widely occurs in various vegetables and fruits, has been reported to possess antioxidant and anti-inflammatory properties. In this study, we investigated the protective effects of AA against lipopolysaccharide (LPS) and d-galactosamine (GalN)-induced FHF and the underlying molecular mechanisms. Our findings suggested that AA treatment effectively protected against LPS/d-GalN-induced FHF by lessening the lethality; decreasing the alanine transaminase and aspartate aminotransferase levels, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α production, malondialdehyde formation, myeloperoxidase level and reactive oxygen species generation (i...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28736281/platycodin-d-suppressed-lps-induced-inflammatory-response-by-activating-lxr%C3%AE-in-lps-stimulated-primary-bovine-mammary-epithelial-cells
#9
Yanan Wang, Xu Zhang, Zhengkai Wei, Jingjing Wang, Yue Zhang, Mingyu Shi, Zhengtao Yang, Yunhe Fu
Platycodin D (PLD), a triterpenoid saponin derived from the root of Platycodon grandiflorum, has been reported to possess anti-inflammatory activity. However, the protective effect of PLD on mastitis has not been reported. In the present study, we aim to investigate the anti-inflammatory feature of PLD on the primary bovine mammary epithelial cells (bMEC) challenged with LPS. The cell viability of bMEC was measured by MTT assay. The quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the gene expression of pro-inflammatory cytokines and western blotting was carried out to measure the expression of LXRα and NF-κB...
July 20, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28736267/gamma-tocopherol-enriched-supplement-reduces-sputum-eosinophilia-and-endotoxin-induced-sputum-neutrophilia-in-volunteers-with-asthma
#10
Allison J Burbank, Charity G Duran, Yinghao Pan, Patricia Burns, Susan Jones, Qing Jiang, Chao Yang, Sha'Leema Jenkins, Heather Wells, Neil Alexis, Mehmet Kesimer, William D Bennett, Haibo Zhou, David B Peden, Michelle L Hernandez
BACKGROUND: We and others have shown that the gamma tocopherol (γT) isoform of vitamin E has multiple anti-inflammatory and antioxidant actions and that γT supplementation reduces eosinophilic and endotoxin (LPS)-induced neutrophilic airway inflammation in animal models and healthy human volunteers. OBJECTIVE: To determine if γT supplementation reduces eosinophilic airway inflammation and acute neutrophilic response to inhaled LPS challenge in volunteers with asthma...
July 20, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28736216/pathogen-induced-tlr4-trif-innate-immune-signaling-in-hematopoietic-stem-cells-promotes-proliferation-but-reduces-competitive-fitness
#11
Hitoshi Takizawa, Kristin Fritsch, Larisa V Kovtonyuk, Yasuyuki Saito, Chakradhar Yakkala, Kurt Jacobs, Akshay K Ahuja, Massimo Lopes, Annika Hausmann, Wolf-Dietrich Hardt, Álvaro Gomariz, César Nombela-Arrieta, Markus G Manz
Bacterial infection leads to consumption of short-lived innate immune effector cells, which then need to be replenished from hematopoietic stem and progenitor cells (HSPCs). HSPCs express pattern recognition receptors, such as Toll-like receptors (TLRs), and ligation of these receptors induces HSPC mobilization, cytokine production, and myeloid differentiation. The underlying mechanisms involved in pathogen signal transduction in HSCs and the resulting biological consequences remain poorly defined. Here, we show that in vivo lipopolysaccharide (LPS) application induces proliferation of dormant HSCs directly via TLR4 and that sustained LPS exposure impairs HSC self-renewal and competitive repopulation activity...
July 19, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28736160/macrophage-phenotype-in-response-to-ecm-bioscaffolds
#12
REVIEW
Luai Huleihel, Jenna L Dziki, Joseph G Bartolacci, Theresa Rausch, Michelle E Scarritt, Madeline C Cramer, Tatiana Vorobyov, Samuel T LoPresti, Ilea T Swineheart, Lisa J White, Bryan N Brown, Stephen F Badylak
Macrophage presence and phenotype are critical determinants of the healing response following injury. Downregulation of the pro-inflammatory macrophage phenotype has been associated with the therapeutic use of bioscaffolds composed of extracellular matrix (ECM), but phenotypic characterization of macrophages has typically been limited to small number of non-specific cell surface markers or expressed proteins. The present study determined the response of both primary murine bone marrow derived macrophages (BMDM) and a transformed human mononuclear cell line (THP-1 cells) to degradation products of two different, commonly used ECM bioscaffolds; urinary bladder matrix (UBM-ECM) and small intestinal submucosa (SIS-ECM)...
July 20, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28736035/propane-2-sulfonic-acid-octadec-9-enyl-amide-a-novel-ppar%C3%AE-%C3%AE-dual-agonist-protects-against-ischemia-induced-brain-damage-in-mice-by-inhibiting-inflammatory-responses
#13
Ying Li, Lanxi Xu, Kaiyue Zeng, Zhentian Xu, Daqin Suo, Lu Peng, Tong Ren, Zhiheng Sun, Wushuang Yang, Xin Jin, Lichao Yang
Propane-2-sulfonic acid octadec-9-enyl-amide (N15), an analogue of oleoylethanolamide (OEA), is a novel PPARα/γ dual agonist. Our previous studies verified the positive effects of OEA on the acute and delayed stages of cerebral ischemia. However, it is not clear whether N15 is effective against ischemic cerebral injury. In the present study, male Kunming mice were subjected to middle cerebral artery occlusion (MCAO). To evaluate its preventive effects, N15 (50, 100 or 200 mg/kg, ip) was administered for 3 days before ischemia...
July 20, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28736034/salvianolic-acid-b-promotes-microglial-m2-polarization-and-rescues-neurogenesis-in-stress-exposed-mice
#14
Jinqiang Zhang, Xiaofang Xie, Mingming Tang, Jing Zhang, Boyang Zhang, Qiuying Zhao, Yue Han, Wan Yan, Cheng Peng, Zili You
Although accumulating evidence suggests that activated microglia are associated with deficits in neurogenesis and contribute to the physiopathology of major depressive disorder, the role of microglia in treating depression remains poorly understood. Our previous study showed that salvianolic acid (SalB) has the regulation of neuroinflammatory responses and antidepressant-like effects. Here, we hypothesized that SalB's therapeutic effects occur because it modulates microglial phenotypes that are associated with neurogenesis...
July 20, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28735965/safety-profile-and-immunologic-responses-of-a-novel-vaccine-against-shigella-sonnei-administered-intramuscularly-intradermally-and-intranasally-results-from-two-parallel-randomized-phase-1-clinical-studies-in-healthy-adult-volunteers-in-europe
#15
Odile Launay, David J M Lewis, Alessandra Anemona, Pierre Loulergue, Jo Leahy, Antonella Silvia Sciré, Anaïs Maugard, Elisa Marchetti, Stefano Zancan, Zhiming Huo, Simona Rondini, Rachid Marhaba, Oretta Finco, Laura B Martin, Jochen Auerbach, Daniel Cohen, Allan Saul, Christiane Gerke, Audino Podda
BACKGROUND: Approximately 164,000 deaths yearly are due to shigellosis, primarily in developing countries. Thus, a safe and affordable Shigella vaccine is an important public health priority. The GSK Vaccines Institute for Global Health (GVGH) developed a candidate Shigella sonnei vaccine (1790GAHB) using the Generalized Modules for Membrane Antigens (GMMA) technology. The paper reports results of 1790GAHB Phase 1 studies in healthy European adults. METHODS: To evaluate the safety and immunogenicity profiles of 1790GAHB, we performed two parallel, phase 1, observer-blind, randomized, placebo-controlled, dose escalation studies in France ("study 1") and the United Kingdom ("study 2") between February 2014 and April 2015 (ClinicalTrials...
July 15, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28735961/binding-of-pmclipsp2-to-microbial-cell-wall-components-and-activation-of-the-propo-activating-system-in-the-black-tiger-shrimp-penaeus-monodon
#16
Narach Khoratanakulchai, Piti Amparyup, Anchalee Tassanakajon
Clip domain serine proteinases (ClipSPs) play an important role in the prophenoloxidase-activating (proPO) system. In the shrimp Penaeus monodon, the ClipSP PmClipSP2 has been previously shown to bind to microbial polysaccharides (LPS and β-1,3-glucan) and likely activates the proPO system. To reveal the binding site of the PmClipSP2 protein, the N-terminal clip domain (Clip-PmClipSP) and C-terminal SP domain (SP-PmClipSP2) were separately cloned. The recombinant proteins were then assayed for their binding properties and involvement in proPO activation...
July 20, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28735521/myd88-erk-nfkb%C3%A2-pathways-and-pro-inflammatory-cytokines-release-in-periodontal-ligament-stem-cells-stimulated-by-porphyromonas%C3%A2-gingivalis
#17
Francesca Diomede, Maria Zingariello, Marcos F X B Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia De Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani
The present study was aimed at investigating whether human Periodontal Ligament Stem Cells (hPDLSCs) were capable of sensing and reacting to lipopolysaccharide from Porphyromonas gingivalis (LPS-G) which is widely recognized as a major pathogen in the development and progression of periodontitis. At this purpose hPDLCs were stimulated with 5 μg/mL LPS-G various times and the expression of toll-like receptor 4 (TLR4) was evaluated. Toll-like receptors (TLRs) play an essential role in innate immune signaling in response to microbial infections, and in particular TLR4, type-I transmembrane proteins, has been shown recognizing LPS-G...
May 24, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28734896/porcine-cd14-gene-silencing-partially-inhibited-the-bacterial-immune-response-mediated-by-tlr4-signaling-pathway
#18
Chaohui Dai, Haifei Wang, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Cluster of differentiation antigen 14 (CD14) is the membrane receptor protein in Toll-like Receptor 4 (TLR4) signaling pathway, which plays an important regulation role in not only innate immune response but also adaptive immune response. In this study, the pig kidney epithelial cell (PK15) line with CD14 gene silencing mediated by lentivirus was established and cells of CD14-RNAi and NC group were exposed to three kinds of Escherichia coli (E. coli F18ab, E. coli F18ac and E. coli K88ac) and LPS. Then qPCR and western blot were used to detect expression levels of TLR4 signaling pathway-related genes...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28734673/somatostatin-protects-human-retinal-pericytes-from-inflammation-mediated-by-microglia
#19
Aurora Mazzeo, Ana I Arroba, Elena Beltramo, Angela M Valverde, Massimo Porta
Diabetic retinopathy (DR) is usually considered a microvascular disease. However, involvement of the neuroretina in the early stages of DR has recently gained major credit. Inflammatory processes, leading to glial activation and neuronal apoptosis, develop early in the retina of diabetic subjects. Pericytes constitute a link between the vascular and the neural retina, play a central role in blood-retinal barrier maintenance, and may influence neuroinflammation. Somatostatin (SST) is a potent neuroprotective factor, which is down-regulated during early DR...
July 19, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28733463/tlr4-and-c5ar-crosstalk-in-dendritic-cells-induces-a-core-regulatory-network-of-rsk2-pi3k%C3%AE-sgk1-and-foxo-transcription-factors
#20
Anouk Zaal, Benjamin Nota, Kat S Moore, Miranda Dieker, S Marieke van Ham, Anja Ten Brinke
Crosstalk between complement component 5a receptors (C5aRs) and TLRs in dendritic cells (DCs) occurs upon pathogen invasion; however, studies on C5aR and TLR crosstalk mainly focused on the modulating effect of C5a on TLR-induced cytokine production. To elucidate the breadth of C5aR and TLR4 crosstalk, the effect of simultaneous treatment with C5a and LPS was investigated in human monocyte-derived DCs (moDCs) 2 h after stimulation using whole transcriptome sequencing analysis. Although the effect of C5a on hallmark genes defining TLR4-induced DC maturation was limited at this time point, RNA sequencing analysis revealed a great variety of novel C5a targets, of which many interfere with TLR4-mediated immune activation...
July 21, 2017: Journal of Leukocyte Biology
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