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https://www.readbyqxmd.com/read/28813612/role-of-zinc-oxide-nanoparticles-in-alleviating-hepatic-fibrosis-and-nephrotoxicity-induced-by-thioacetamide-in-rats
#1
Samir Ae Bashandy, Abdulaziz Alaamer, Sherif Abdelmawgoud Moussa, Enayat Omara
The present research studied the influence of zinc oxide nanoparticles (ZnO-NPs; 5mg, 7.5mg, 10 mg/Kg IP) on the liver and kidney injuries motivated by thioacetamide (TAA; 100mg/Kg IP).Each treatment was carried out three times weekly for eight weeks .ZnO-NPs relieved the decrease of hepatic or renal reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) induced by TAA. Moreover, ZnO-NPs lowered tissues malondialdehyde (MDA, an indicator for lipid peroxidation). TAA treatment led to a significant increase in plasma inflammatory markers (TNF-α, IL-6), liver enzymes (Gamma-glutamyltransferase (GGT), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and kidney function parameters (Creatinine, urea, uric acid)...
August 16, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28813510/construction-of-novel-pjrd215-derived-plasmids-using-chloramphenicol-acetyltransferase-cat-gene-as-a-selection-marker-for-acidithiobacillus-caldus
#2
Rui Wang, Chunmao Lin, Jianqiang Lin, Xin Pang, Xiangmei Liu, Chengjia Zhang, Jianqun Lin, Linxu Chen
BACKGROUND: Acidithiobacillus caldus, a Gram-negative, chemolithotrophic sulfur-oxidizing bacterium, is widely applied in bioleaching. The absence of an ideal selection marker has become a major obstacle to achieve high efficiency of the gene transfer system for A. caldus. Plasmid pJRD215, widely used in Acidithiobacillus spp., has severe drawbacks in molecular manipulations and potential biosafety issues due to its mobility. Therefore, finding a new selection marker and constructing new plasmids have become an urgent and fundamental work for A...
2017: PloS One
https://www.readbyqxmd.com/read/28813459/%C3%AE-2-microglobulin-gene-duplication-in-cetartiodactyla-remains-intact-only-in-pigs-and-possibly-confers-selective-advantage-to-the-species
#3
Thong Minh Le, Quy Van Chanh Le, Dung Minh Truong, Hye-Jeong Lee, Min-Kyeung Choi, Hyesun Cho, Hak-Jae Chung, Jin-Hoi Kim, Jeong-Tae Do, Hyuk Song, Chankyu Park
Several β2-microglobulin (B2M) -bound protein complexes undertake key roles in various immune system pathways, including the neonatal Fc receptor (FcRn), cluster of differentiation 1 (CD1) protein, non-classical major histocompatibility complex (MHC), and well-known MHC class I molecules. Therefore, the duplication of B2M may lead to an increase in the biological competence of organisms to the environment. Based on the pig genome assembly SSC10.2, a segmental duplication of ~45.5 kb, encoding the entire B2M protein, was identified in pig chromosome 1...
2017: PloS One
https://www.readbyqxmd.com/read/28813445/transition-of-the-intestinal-microbiota-of-cats-with-age
#4
Hiroaki Masuoka, Kouya Shimada, Tomoyo Kiyosue-Yasuda, Masaharu Kiyosue, Yukie Oishi, Seiji Kimura, Yuji Ohashi, Tomohiko Fujisawa, Kozue Hotta, Akio Yamada, Kazuhiro Hirayama
The transition of intestinal microbiota with age has been well described in humans. However, the age-related changes in intestinal microbiota of cats have not been well studied. In the present study, we investigated the composition of intestinal microbiota of cats in 5 different age groups (pre-weanling, weanling, young, aged, senile) with a culture-based method. For lactobacilli and bifidobacteria, we also quantified with molecular-based method, real-time PCR. The results suggested that the composition of the feline intestinal microbiota changes with age, while the changes were different from those of humans and dogs...
2017: PloS One
https://www.readbyqxmd.com/read/28813431/skeleton-of-an-unusual-cat-sized-marsupial-relative-metatheria-marsupialiformes-from-the-middle-eocene-lutetian-44-43-million-years-ago-of-turkey
#5
A Murat Maga, Robin M D Beck
We describe a near-complete, three-dimensionally preserved skeleton of a metatherian (relative of modern marsupials) from the middle Eocene (Lutetian: 44-43 million years ago) Lülük member of the Uzunçarşıdere Formation, central Turkey. With an estimated body mass of 3-4 kg, about the size of a domestic cat (Felis catus) or spotted quoll (Dasyurus maculatus), it is an order of magnitude larger than the largest fossil metatherians previously known from the Cenozoic of the northern hemisphere. This new taxon is characterised by large, broad third premolars that probably represent adaptations for hard object feeding (durophagy), and its craniodental morphology suggests the capacity to generate high bite forces...
2017: PloS One
https://www.readbyqxmd.com/read/28813176/validating-the-concept-of-copd-control-a-real-world-cohort-study-from-the-united-kingdom
#6
Anjan Nibber, Alison Chisholm, Juan José Soler-Cataluña, Bernardino Alcazar, David Price, Marc Miravitlles
The concept of Chronic Obstructive Pulmonary Disease (COPD) control has been developed to inform therapeutic decision-making. We explored the validity of a definition of COPD control in a representative population of patients with COPD in the United Kingdom. Electronic medical records and linked COPD questionnaire data from the Optimum Patient Care Research Database were used to characterize control status. Patients were aged ≥40 years, with spirometry-confirmed COPD, current or ex-smokers, and continuous records throughout the study period...
August 16, 2017: COPD
https://www.readbyqxmd.com/read/28812696/skeletal-trauma-reflects-hunting-behaviour-in-extinct-sabre-tooth-cats-and-dire-wolves
#7
Caitlin Brown, Mairin Balisi, Christopher A Shaw, Blaire Van Valkenburgh
Skeletal-injury frequency and distribution are likely to reflect hunting behaviour in predatory vertebrates and might therefore differ between species with distinct hunting modes. Two Pleistocene predators from the Rancho La Brea asphalt seeps, the sabre-tooth cat, Smilodon fatalis, and dire wolf, Canis dirus, represent ambush and pursuit predators, respectively. On the basis of a collection of over 1,900 pathological elements, the frequency of traumatic injury across skeletal elements in these two species was calculated...
April 10, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28811799/diversity-physiochemical-and-phylogenetic-analyses-of-bacteria-isolated-from-various-drinking-water-sources
#8
Neveen H Eid, Huda A Al Doghaither, Taha A Kumosani, Munazza Gull
OBJECTIVE: To evaluate the indigenous bacterial strains of drinking water from the most commercial water types including bottled and filtered water that are currently used in Saudi Arabia. METHODS: Thirty randomly selected commercial brands of bottled water were purchased from Saudi local markets. Moreover, samples from tap water and filtered water were collected in sterilized glass bottles and stored at 4°C. Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28811686/influence-of-the-flavonoid-quercetin-on-antioxidant-status-lipid-peroxidation-and-histopathological-changes-in-hyperammonemic-rats
#9
Sivamani Kanimozhi, Pakkiri Bhavani, Perumal Subramanian
We have studied the ability of quercetin (a bioflavonoid) in tackling oxidative stress to alleviate the symptoms during ammonium chloride-induced hyperammonemia. Hyperammonemia was induced by the treatment of ammonium chloride (AC) 100 mg/kg b.w for 56 days. Hyperammonemic rats exhibited reduced urea (in plasma) and increased ammonia (in blood), uric acid (in plasma), creatinine (in serum), oxidative stress markers (thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP) and decreased levels of antioxidants (enzymatic and non-enzymatic) superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) in plasma and tissues (liver and brain) vitamins E and C (in plasma))...
July 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28811626/outbreak-and-genotyping-of-canine-distemper-virus-in-captive-siberian-tigers-and-red-pandas
#10
He Zhang, Fen Shan, Xia Zhou, Bing Li, Jun-Qiong Zhai, Shu-Zhan Zou, Meng-Fan Wu, Wu Chen, Shao-Lun Zhai, Man-Lin Luo
In this study, four canine distemper virus (CDV) strains were isolated from captive Siberian tigers (Panthera tigris altaica) and red pandas (Ailurus fulgens) during two separate CDV outbreaks in a zoo in Guangdong province, China. Sequence alignment and phylogenetic analyses based on the full-length hemagglutinin (H) and fusion (F) genes showed that they were closely identical to genotype Asia-1. Prior to confirmation of CDV in Siberian tigers, to control spread of the disease, a live attenuated combination CDV vaccine was used among almost all carnivore animals except for red pandas in which another recombinant combination CDV vaccine was used...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811394/overview-and-current-management-of-computerized-adaptive-testing-in-licensing-certification-examinations
#11
Dong Gi Seo
Computerized adaptive testing (CAT) has been implemented in the high stakes examinations such as National Council Licensure Examination-Registered Nurses (NCLEX-RN) in the United States since 1994. After that, The National Registry Emergency Medical Technician (NREMT) in the United States adopted a CAT to certify an Emergency Medical Technician (EMT) in 2007. It aimed at introducing CAT for the implementation in the medical health licensing examinations. Most CATs are based on the item response theory (IRT) which hypothesizes that both examinee and item has own characteristics that does not change...
July 26, 2017: Journal of Educational Evaluation for Health Professions
https://www.readbyqxmd.com/read/28810923/the-chemokine-fractalkine-cx3cl1-attenuates-h2o2-induced-demyelination-in-cerebellar-slices
#12
Sinead A O'Sullivan, Kumlesh K Dev
BACKGROUND: Fractalkine/CX3CR1 signalling has been implicated in many neurodegenerative and neurological diseases of the central nervous system (CNS). This signalling pathway plays an important role in regulating reactive oxygen species (ROS), as well as itself being altered in conditions of oxidative stress. Here, we investigated the effects of recombinant fractalkine (rCX3CL1) in models of hydrogen peroxide (H2O2)-induced demyelination and astrocyte toxicity, within organotypic cerebellar slice cultures...
August 15, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28810700/a-single-mini-barcode-test-to-screen-for-australian-mammalian-predators-from-environmental-samples
#13
Elodie Modave, Anna J MacDonald, Stephen D Sarre
Identification of species from trace samples is now possible through the comparison of diagnostic DNA fragments against reference DNA sequence databases. DNA detection of animals from non-invasive samples, such as predator faeces (scats) that contain traces of DNA from their species of origin, has proved to be a valuable tool for the management of elusive wildlife. However, application of this approach can be limited by the availability of appropriate genetic markers. Scat DNA is often degraded, meaning that longer DNA sequences, including standard DNA barcoding markers, are difficult to recover...
August 1, 2017: GigaScience
https://www.readbyqxmd.com/read/28810533/deltamethrin-induced-oxidative-stress-in-kidney-and-brain-of-rats-protective-effect-of-artemisia-campestris-essential-oil
#14
Mongi Saoudi, Riadh Badraoui, Houda Bouhajja, Marwa Ncir, Fatma Rahmouni, Malek Grati, Kamel Jamoussi, Abdelfattah El Feki
Artemisia campestris (Asteraceae) is widely used in traditional medicine in Southern Tunisia as a decoction for its antivenom, anti-inflammatory, antirheumatic, and antimicrobial activities. A. campestris essential oil (ACEO) was obtained by hydrodistillation from the aerial parts, since it has beneficial and therapeutic effects. Deltamethrin is a synthetic pyrethroid with broad spectrum activities against acaricides and insects and widely used for veterinary and agricultural purposes. Exposure to deltamethrin leads to nephrotoxic and neurotoxic side effects for human and many species including birds and fish...
August 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28810519/the-beneficial-role-of-naringin-a-citrus-bioflavonoid-against-oxidative-stress-induced-neurobehavioral-disorders-and-cognitive-dysfunction-in-rodents-a-systematic-review-and-meta-analysis
#15
REVIEW
Gollapalle Lakshminarayanashastry Viswanatha, H Shylaja, Yogananda Moolemath
OBJECTIVES: Naringin is a bioflavonoid, very abundantly found in citrus species. In literature, naringin has been scientifically well documented for its beneficial effects in various neurological disorders. In this systematic review and meta-analysis, we have made an attempt to correlate the protective role of naringin against oxidative stress-induced neurological disorders in rodents. METHODS: The systematic search was performed using electronic databases; the search was mainly focused on the role of naringin in oxidative stress-induced neuropathological conditions in rodents...
August 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28809861/high-frequency-measurement-of-depressive-severity-in-a-patient-treated-for-severe-treatment-resistant-depression-with-deep-brain-stimulation
#16
S Sani, J Busnello, R Kochanski, Y Cohen, R D Gibbons
Although there have been previous studies of deep-brain stimulation (DBS), we present, to our knowledge, the first example of high-frequency depressive severity measurement-based DBS treatment in particular and psychiatric treatment in general. Daily post-surgical e-mail prompts for a period of 6 months resulted in 93 administrations of a computerized adaptive test (CAT) of depression severity (CAT-Depression Inventory or CAT-DI) via the internet. There was an average of 3.37 weekly measurements with an average separation of 2...
August 15, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28809125/post-chemotherapy-perforation-in-cats-with-discrete-intermediate-or-large-cell-gastrointestinal-lymphoma
#17
Zachary Crouse, Brenda Phillips, Andi Flory, Jennifer Mahoney, Keith Richter, Linda Kidd
Objectives Gastrointestinal (GI) perforation is a well-described complication of GI lymphoma in people, commonly occurring within days of initiation of chemotherapy. There are no studies documenting the prevalence of GI perforation in cats with intermediate- or large-cell GI lymphoma or whether it is associated with induction of chemotherapy. The objectives of this study were to document the prevalence and timing of post-chemotherapy perforation in cats with discrete GI masses caused by intermediate- or large-cell lymphoma...
August 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28809124/measurement-of-the-normal-feline-pituitary-gland-in-brachycephalic-and-mesocephalic-cats
#18
Thomas Christian Häußler, Kerstin Heike von Pückler, Cetina Thiel, Sylke Enderlein, Klaus Failing, Nele Ondreka, Martin Kramer, Martin Jürgen Schmidt
Objectives The purpose of this study was to determine differences in normal feline pituitary dimensions in brachycephalic and mesocephalic cats as a basis for establishing cryohypophysectomy in cats. Methods Measurements were performed on sagittal T2-weighted and transverse post-gadolinium T1-weighted or T1 3D fast field echo-weighted MRI images. A total of 32 brachycephalic and 27 mesocephalic cats were examined. Inter-observer reproducibility was assessed by t-test and Bland-Altman analysis. Results The cats were 0...
August 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28808815/physiological-responses-and-antioxidant-enzyme-changes-in-sulla-coronaria-inoculated-by-cadmium-resistant-bacteria
#19
Manel Chiboub, Salwa Harzalli Jebara, Omar Saadani, Imen Challougui Fatnassi, Souhir Abdelkerim, Moez Jebara
Plant growth promoting bacteria (PGPB) may help to reduce the toxicity of heavy metals on plants growing in polluted soils. In this work, Sulla coronaria inoculated with four Cd resistant bacteria (two Pseudomonas spp. and two Rhizobium sullae) were cultivated in hydroponic conditions treated by Cd; long time treatment 50 µM CdCl2 for 30 days and short time treatment; 100 µM CdCl2 for 7 days. Results showed that inoculation with Cd resistant PGPB enhanced plant biomass, thus shoot and root dry weights of control plants were enhanced by 148 and 35% respectively after 7 days...
August 14, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28808666/lipidomic-evaluation-of-feline-neurologic-disease-after-aav-gene-therapy
#20
Heather L Gray-Edwards, Xuntian Jiang, Ashley N Randle, Amanda R Taylor, Taylor L Voss, Aime K Johnson, Victoria J McCurdy, Miguel Sena-Esteves, Daniel S Ory, Douglas R Martin
GM1 gangliosidosis is a fatal lysosomal disorder, for which there is no effective treatment. Adeno-associated virus (AAV) gene therapy in GM1 cats has resulted in a greater than 6-fold increase in lifespan, with many cats remaining alive at >5.7 years of age, with minimal clinical signs. Glycolipids are the principal storage product in GM1 gangliosidosis whose pathogenic mechanism is not completely understood. Targeted lipidomics analysis was performed to better define disease mechanisms and identify markers of disease progression for upcoming clinical trials in humans...
September 15, 2017: Molecular Therapy. Methods & Clinical Development
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