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https://www.readbyqxmd.com/read/28318332/abnormality-of-adipokines-and-endothelial-dysfunction-in-mexican-obese-adolescents-with-insulin-resistance
#1
Maria Del Carmen Ortiz Segura, Blanca Estela Del Río Navarro, Benjamín Antonio Rodríguez Espino, Laurence A Marchat, Fausto Sánchez Muñoz, Santiago Villafaña, Enrique Hong, Fabián Meza-Cuenca, Patrick Mailloux Salinas, Francisco Bolaños-Jiménez, Elena Zambrano, Abel Armando Arredondo-López, Guadalupe Bravo, Fengyang Huang
PURPOSE: The aim of this study was to investigate the possible relationship among insulin resistance (IR), endothelial dysfunction, and alteration of adipokines in Mexican obese adolescents and their association with metabolic syndrome (MetS). MATERIALS AND METHODS: Two hundred and twenty-seven adolescents were classified according to the body mass index (BMI) (control: N=104; obese: N=123) and homeostasis model of the assessment-insulin resistance index (HOMA-IR) (obese with IR: N=65)...
March 20, 2017: Endocrine Research
https://www.readbyqxmd.com/read/28317350/-effects-of-dentin-demineralization-and-bond-strength-properties-of-bioactive-glass-containing-resin-adhesive
#2
Wang Yu, Gong Ling, Ji Yan, Zheng Lichun, Chen Song
OBJECTIVE: This study investigated the shear bond strength and the ability of resin adhesive containing different amounts of bioactive glass (BG) to prevent dentin demineralization. METHODS: BG bonds at 30%, 40%, and 50% concentrations comprised a mixture of coupling agents modifying BG and Single Bond 2. Forty permanent premolars extracted for orthodontic treatment were randomly divided into four groups, namely, control group (BG-free), 30% BG group, 40% BG group, and 50% BG group...
August 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28316328/reoperation-after-cyst-excision-with-hepaticojejunostomy-for-choledochal-cysts-our-experience-in-18-cases
#3
Qingfeng Sheng, Zhibao Lv, Weijue Xu, Xianmin Xiao, Jiangbin Liu, Yibo Wu
BACKGROUND Complete cyst excision with biliary reconstruction is the treatment of choice for choledochal cyst (CC). The aim of this article is to review our experience in patients who underwent reoperation between January 1995 and December 2014. MATERIAL AND METHODS The records of 18 patients (female/male, 15/3) were retrospectively analyzed including age, sex, cyst type, initial procedure, lab and imaging findings, indications for reoperation, intraoperative findings, and results of reoperation. The median follow-up period was 6 years...
March 20, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28302668/sulphated-glycosaminoglycans-support-an-assortment-of-planarian-rhabdite-structures
#4
Matthew J Hayes
Planaria are soft-bodied, bilateral flatworms of the phylum Playhelminthes. They are covered in cilia and use ciliary-gliding to traverse the substratum while hunting. Their body surface is covered in a layer of viscous slime primarily derived from specialised secretory granules known as rhabdites. The slime must somehow stay associated with the surface of the animal in aqueous environments whilst also lubricating the interface of the animal and the surfaces over which the animal moves. The slime prevents damage to the animal's soft body and also contributes to adhesion to the substratum...
March 16, 2017: Biology Open
https://www.readbyqxmd.com/read/28302626/sting-irf3-triggers-endothelial-inflammation-in-response-to-free-fatty-acid-induced-mitochondrial-damage-in-diet-induced-obesity
#5
Yun Mao, Wei Luo, Lin Zhang, Weiwei Wu, Liangshuai Yuan, Hao Xu, Juhee Song, Keigi Fujiwara, Jun-Ichi Abe, Scott A LeMaire, Xing Li Wang, Ying H Shen
OBJECTIVE: Metabolic stress in obesity induces endothelial inflammation and activation, which initiates adipose tissue inflammation, insulin resistance, and cardiovascular diseases. However, the mechanisms underlying endothelial inflammation induction are not completely understood. Stimulator of interferon genes (STING) is an important molecule in immunity and inflammation. In the present study, we sought to determine the role of STING in palmitic acid-induced endothelial activation/inflammation...
March 16, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28301131/bio-inspired-nano-featured-substrates-suitable-environment-for-bone-regeneration
#6
Hassan Rammal, Marie Dubus, Léa Aubert, Fany Reffuveille, Dominique Laurent Maquin, Christine Terryn, Pierre Schaaf, Halima Alem, Grégory Francius, Fabienne Quilès, Sophie C Gangloff, Fouzia Boulmedais, Halima Kerdjoudj
Bone mimicking coatings provide a complex microenvironment in which material, through its inherent properties (such as nanostructure and composition), affects the commitment of stem cells into bone lineage and the production of bone tissue regulating factors, required for bone healing and regeneration. Herein, a bioactive mineral/biopolymers composite made of calcium phosphate/chitosan and hyaluronic acid (CaP-CHI-HA) was elaborated using a versatile simultaneous spray coating of interacting species. The resulting CaP-CHI-HA coating was mainly constituted of bioactive, carbonated and crystalline hydroxyapatite, with 277 ± 98 nm of roughness, 1 μm of thickness and 2...
March 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28299945/in-vitro-and-in-vivo-cholesterol-lowering-ability-of-lactobacillus-pentosus-kf923750
#7
F Bendali, K Kerdouche, S Hamma-Faradji, D Drider
Lactobacillus pentosus KF923750 was characterised for probiotic related properties and then characterised for cholesterol uptake in vitro as well as in vivo using rabbits fed a high-cholesterol diet. The survival percentage of L. pentosus KF923750 was 100% at pH 3, 52.18% at pH 2 and 36.21% at pH 2 plus pepsin. Similarly, this strain appeared resistant to bile (0.1% [98.42%], 0.3% [88.52%], 0.5% [75.60%] and 1% [71.15%]), after 4 h exposure. Moreover, L. pentosus KF923750 controlled growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 through the production of a bacteriocin-like inhibitory substance and anti-adhesive capabilities...
March 16, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28297834/-effect-of-inhibiting-perivascular-adhesion-on-intimal-hyperplasia-of-the-vein-grafts-in-rabbits
#8
M K Guo, J X Zhu, C M Han, T Bao, D Yang, P Yu, Y F Duan, F L Fan, H G Zhang, H J Tian
Objective: To investigate the effect of preventing perivascular adhesion with topical application of sodium hyaluronate on intimal hyperplasia of the vein grafts in rabbits. Methods: Twenty-four male New Zealand white rabbits, aged 5 months, were randomly divided into 2 groups: Group A and B (n=12 rabbits per group). Artery defect model was established by cutting about 1 cm artery from the middle part of the dissociated left common carotid artery. A section about 3 cm was cut from the right external jugular vein, and the harvested vein was inverted and end-to-end anastomosed to the artery defect...
March 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28297678/roles-for-cell-cell-adhesion-and-contact-in-obesity-induced-hepatic-myeloid-cell-accumulation-and-glucose-intolerance
#9
Yasutaka Miyachi, Kyoichiro Tsuchiya, Chikara Komiya, Kumiko Shiba, Noriko Shimazu, Shinobu Yamaguchi, Michiyo Deushi, Mizuko Osaka, Kouji Inoue, Yuta Sato, Sayaka Matsumoto, Junichi Kikuta, Kenjiro Wake, Masayuki Yoshida, Masaru Ishii, Yoshihiro Ogawa
Obesity promotes infiltration of inflammatory cells into various tissues, leading to parenchymal and stromal cell interaction and development of cellular and organ dysfunction. Liver sinusoidal endothelial cells (LSECs) are the first cells that contact portal blood cells and substances in the liver, but their functions in the development of obesity-associated glucose metabolism remain unclear. Here, we find that LSECs are involved in obesity-associated accumulation of myeloid cells via VLA-4-dependent cell-cell adhesion...
March 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28297575/selective-inhibition-of-adam17-efficiently-mediates-glycoprotein-ib%C3%AE-retention-during-ex-vivo-generation-of-human-induced-pluripotent-stem-cell-derived-platelets
#10
Shinji Hirata, Takahiko Murata, Daisuke Suzuki, Sou Nakamura, Ryoko Jono-Ohnishi, Hidenori Hirose, Akira Sawaguchi, Satoshi Nishimura, Naoshi Sugimoto, Koji Eto
Donor-independent platelet concentrates for transfusion can be produced in vitro from induced pluripotent stem cells (iPSCs). However, culture at 37°C induces ectodomain shedding on platelets of glycoprotein Ibα (GPIbα), the von Willebrand factor receptor critical for adhesive function and platelet lifetime in vivo, through temperature-dependent activation of a disintegrin and metalloproteinase 17 (ADAM17). The shedding can be suppressed by using inhibitors of panmetalloproteinases and possibly of the upstream regulator p38 mitogen-activated protein kinase (p38 MAPK), but residues of these inhibitors in the final platelet products may be accompanied by harmful risks that prevent clinical application...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28296132/polyvalent-2d-entry-inhibitors-for-pseudorabies-and-african-swine-fever-virus
#11
Benjamin Ziem, Jessica Rahn, Ievgen Donskyi, Kim Silberreis, Luis Cuellar, Jens Dernedde, Günther Keil, Thomas C Mettenleiter, Rainer Haag
African swine fever virus (ASFV) is one of the most dangerous viruses for pigs and is endemic in Africa but recently also spread into the Russian Federation and the Eastern border of the EU. So far there is no vaccine or antiviral drug available to curtail the infection. Thus, control strategies based on novel inhibitors are urgently needed. Another highly relevant virus infection in pigs is Aujeszky's disease caused by the alphaherpesvirus pseudorabies virus (PrV). This article reports the synthesis and biological evaluation of novel extracellular matrix-inspired entry inhibitors based on polyglycerol sulfate-functionalized graphene sheets...
March 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28296083/immobilization-of-pt-nanoparticles-via-rapid-and-reusable-electropolymerization-of-dopamine-on-tio2-nanotube-arrays-for-reversible-sers-substrates-and-nonenzymatic-glucose-sensors
#12
Jingsheng Cai, Jianying Huang, Mingzheng Ge, James Iocozzia, Zhiqun Lin, Ke-Qin Zhang, Yuekun Lai
Inspired by mussel-adhesion phenomena in nature, polydopamine (PDA) coatings are a promising route to multifunctional platforms for decorating various materials. The typical self-polymerization process of dopamine is time-consuming and the coatings of PDA are not reusable. Herein, a reusable and time-saving strategy for the electrochemical polymerization of dopamine (EPD) is reported. The PDA layer is deposited on vertically aligned TiO2 nanotube arrays (NTAs). Owing to the abundant catechol and amine groups in the PDA layer, uniform Pt nanoparticles (NPs) are deposited onto the TiO2 NTAs and can effectively prevent the recombination of electron-hole pairs generated from photo-electrocatalysis and transfer the captured electrons to participate in the photo-electrocatalytic reaction process...
March 10, 2017: Small
https://www.readbyqxmd.com/read/28294475/sodium-tanshinone-iia-sulfonate-prevents-hypoxic-trophoblast-induced-endothelial-cell-dysfunction-via-targeting-hmgb1-release
#13
Min Zhao, Yaling Feng, Jianping Xiao, Jie Liang, Yongxiang Yin, Daozhen Chen
Preeclampsia (PE) is a serious blood pressure disorder of pregnancy. Systemic endothelial cell dysfunction, a hallmark of PE, is previously estimated to be induced by hypoxic trophoblast high mobility group box 1 (HMGB1). In the present study, we investigated the protective effect of sodium tanshinone IIA sulfonate (STS), the soluble form of tanshinone IIA isolated from danshen, against hypoxic trophoblast HMGB1-induced human umbilical vein endothelial cell (HUVEC) dysfunction. Our results showed that HMGB1 expression and release were significantly decreased in STS-treated hypoxic JEG-3 cells...
February 16, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28293518/the-use-of-a-hyaluronic-acid-carboxymethylcellulose-membrane-as-an-adhesion-barrier-during-nipple-delay-before-nipple-preserving-mastectomy
#14
Andreas M Lamelas, Eric M Jablonka, Marco A Harmaty, Philip J Torina
The desire for nipple-preserving mastectomies has increased over the past decade as studies have proven that the procedure has comparable oncologic risk as a traditional mastectomy in both therapeutic and prophylactic cases. Partial or complete nipple necrosis is a well-known complication of this procedure with rates in the literature ranging between 1% and 9%. In high-risk patients, surgeons are performing a delay procedure before the mastectomy to help improve nipple vascularity and decrease necrosis rates...
December 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28293103/protective-influence-of-hyaluronic-acid-on-focal-adhesion-kinase-activity-in-human-skin-fibroblasts-exposed-to-ethanol
#15
Magdalena Donejko, Edyta Rysiak, Elżbieta Galicka, Robert Terlikowski, Edyta Katarzyna Głażewska, Andrzej Przylipiak
AIM: The aim of this study was to evaluate the effect of ethanol and hyaluronic acid (HA) on cell survival and apoptosis in cultured human skin fibroblasts. Regarding the mechanism of ethanol action on human skin fibroblasts, we investigated cell viability and apoptosis, expression of focal adhesion kinase (FAK), and the influence of HA on those processes. MATERIALS AND METHODS: Studies were conducted in confluent human skin fibroblast cultures that were treated with 25 mM, 50 mM, and 100 mM ethanol or with ethanol and 500 µg/mL HA...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28292897/inhibition-of-atherogenesis-by-the-cop9-signalosome-subunit-5-in-vivo
#16
Yaw Asare, Miriam Ommer, Florence A Azombo, Setareh Alampour-Rajabi, Marieke Sternkopf, Maryam Sanati, Marion J Gijbels, Corinna Schmitz, Dzmitry Sinitski, Pathricia V Tilstam, Hongqi Lue, André Gessner, Denise Lange, Johannes A Schmid, Christian Weber, Martin Dichgans, Joachim Jankowski, Ruggero Pardi, Menno P J de Winther, Heidi Noels, Jürgen Bernhagen
Constitutive photomorphogenesis 9 (COP9) signalosome 5 (CSN5), an isopeptidase that removes neural precursor cell-expressed, developmentally down-regulated 8 (NEDD8) moieties from cullins (thus termed "deNEDDylase") and a subunit of the cullin-RING E3 ligase-regulating COP9 signalosome complex, attenuates proinflammatory NF-κB signaling. We previously showed that CSN5 is up-regulated in human atherosclerotic arteries. Here, we investigated the role of CSN5 in atherogenesis in vivo by using mice with myeloid-specific Csn5 deletion...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28292367/myofibroblast-mediated-contraction
#17
Wae M Al Kattan, Seham F Alarfaj, Bayan M Alnooh, Hadeel F Alsaif, Hayfa S Alabdul Karim, Noha M Al-Qattan, Mohammad M Al-Qattan, Amel A F El-Sayed
Myofibroblast-mediated contraction is viewed as a cycle of four steps. The first step is stimulation of myofibroblasts by lysophospholipids leading to the activation of G proteins and ending with contraction of the actin-myosin complex. The next step is the transmission of the intracellular contractile force at the focal adhesions of myofibroblasts; a step that involves talin, vinculin, paxillin, Hic-5, and the integrin receptors. In the third step, fibronectin will act as the extracellular link between the integrin receptors and the extracellular collagen...
January 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/28289759/thermal-imaging-of-the-pulp-during-residual-adhesive-removal
#18
Gökmen Kurt, Nisa Gül, Özgür Er, Gülşen Çakmak, Emre Bendeş, Veysel Aslantaş
OBJECTIVE: The aim of this study was to evaluate the temperature changes of the pulpal area during different adhesive clean-up procedures. MATERIALS AND METHODS: A total of 80 freshly extracted adult maxillary premolar teeth were divided into four groups. Adhesive clean-up was performed with 6- and 12-fluted tungsten carbide burs (TCB) using low- and high-speed handpieces with air or water cooling after bracket debonding. The temperature changes and cool down times were evaluated with a thermal camera...
March 13, 2017: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
https://www.readbyqxmd.com/read/28288851/clinically-distinct-presentations-of-copper-deficiency-myeloneuropathy-and-cytopenias-in-a-patient-using-excessive-zinc-containing-denture-adhesive
#19
Sahara J Cathcart, Alina G Sofronescu
OBJECTIVES: While copper deficiency has long been known to cause cytopenias, copper deficiency myeloneuropathy is a more recently described entity. Here, we present the case of two clinically distinct presentations of acquired copper deficiency syndromes secondary to excessive use of zinc-containing denture adhesive over five years: myeloneuropathy and severe macrocytic anemia and neutropenia. METHODS: Extensive laboratory testing and histologic evaluation of the liver and bone marrow, were necessary to rule out other disease processes and establish the diagnosis of copper deficiency...
March 10, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28287510/adsc-sheet-transplantation-to-prevent-stricture-after-extended-esophageal-endoscopic-submucosal-dissection
#20
Guillaume Perrod, Laetitia Pidial, Sophie Camilleri, Alexandre Bellucci, Amaury Casanova, Thomas Viel, Bertrand Tavitian, Chirstophe Cellier, Olivier Clément, Gabriel Rahmi
In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The development of diversified technologies combining adipose tissue-derived stromal cells (ADSCs) with various biomaterials has led to the construction of numerous types of tissue-engineered substitutes, such as bone, cartilage, and adipose tissues from rodent, porcine, or human ADSCs. Extended esophageal endoscopic submucosal dissection (ESD) is responsible for esophageal stricture formation...
February 10, 2017: Journal of Visualized Experiments: JoVE
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