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https://www.readbyqxmd.com/read/28231085/extraction-identification-and-photo-physical-characterization-of-persimmon-diospyros-kaki-l-carotenoids
#1
Khalil Zaghdoudi, Orleans Ngomo, Régis Vanderesse, Philippe Arnoux, Bauyrzhan Myrzakhmetov, Céline Frochot, Yann Guiavarc'h
Carotenoid pigments were extracted and purified from persimmon fruits using accelerated solvent extraction (ASE). Eleven pigments were isolated and five of them were clearly identified as all-trans-violaxanthine, all-trans-lutein, all-trans-zeaxanthin all-trans-cryptoxanthin and all-trans-β-carotene. Absorption and fluorescence spectra were recorded. To evaluate the potential of ¹O₂ quenching of the purified carotenoids, we used a monocarboxylic porphyrin (P1COOH) as the photosensitizer to produce ¹O₂...
January 12, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28221946/effect-of-power-levels-on-inactivation-of-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-monocytogenes-in-tomato-paste-using-915-megahertz-microwave-and-ohmic-heating
#2
Sang-Soon Kim, Hye-Jung Sung, Hyo-Sun Kwak, In-Sun Joo, Jeong-Su Lee, Gwangpyo Ko, Dong-Hyun Kang
The effect of power levels on inactivation of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes in tomato paste was investigated using 915-MHz microwave heating (MW) and ohmic heating (OH). Heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kW MW and corresponding OH. GInaFit was used to analyze pathogen inactivation. The heating uniformity of MW-treated samples was inferior to that of OH-treated samples at low power levels of 1...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28219525/acclimation-strategy-of-rhodopseudomonas-palustris-to-high-light-irradiance
#3
Dayana Muzziotti, Alessandra Adessi, Cecilia Faraloni, Giuseppe Torzillo, Roberto De Philippis
The ability of Rhodopseudomonas palustris cells to rapidly acclimate to high light irradiance is an essential issue when cells are grown under sunlight. The aim of this study was to investigate the photo-acclimation process in Rhodopseudomonas palustris 42OL under different culturing conditions: (i) anaerobic (AnG), (ii) aerobic (AG), and (iii) under H2-producing (HP) conditions both at low (LL) and high light (HL) irradiances. The results obtained clearly showed that the photosynthetic unit was significantly affected by the light irradiance at which Rp...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28202360/lycopene-resveratrol-vitamin-c-and-feso4-increase-damage-produced-by-pro-oxidant-carcinogen-4-nitroquinoline-1-oxide-in-drosophila-melanogaster-xenobiotic-metabolism-implications
#4
I E Dueñas-García, M E Heres-Pulido, M R Arellano-Llamas, J De la Cruz-Núñez, V Cisneros-Carrillo, C S Palacios-López, L Acosta-Anaya, L F Santos-Cruz, L Castañeda-Partida, A Durán-Díaz
4-nitroquinoline-1-oxide (4-NQO) is a pro-oxidant carcinogen bioactivated by xenobiotic metabolism (XM). We investigated if antioxidants lycopene [0.45, 0.9, 1.8 μM], resveratrol [11, 43, 172 μM], and vitamin C [5.6 mM] added or not with FeSO4 [0.06 mM], modulate the genotoxicity of 4-NQO [2 mM] with the Drosophila wing spot test standard (ST) and high bioactivation (HB) crosses, with inducible and high levels of cytochromes P450, respectively. The genotoxicity of 4-NQO was higher when dissolved in an ethanol - acetone mixture...
February 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28196351/fabrication-and-functional-attributes-of-lipidic-nanoconstructs-of-lycopene-an-innovative-endeavour-for-enhanced-cytotoxicity-in-mcf-7-breast-cancer-cells
#5
Ashay Jain, Gajanand Sharma, Varun Kushwah, Kanika Thakur, Gargi Ghoshal, Bhupinder Singh, Sanyog Jain, U S Shivhare, O P Katare
The present study investigates the anticancer efficacy of lycopene loaded lipidic nanostructured particles. With a homogenization-evaporation technique, lycopene loaded solid lipid nanoparticles (LYC-SLNs) were fabricated with different ratios of biocompatible viz. compritol ATO 888 and gelucire, and evaluated for their micromeretics properties, in vitro release, in vitro cytotoxicity, cellular uptake and apoptosis induced in MCF-7 cells. Effects of anticancer potential of LYC-SLNs on the efficacy of methotrexate (MTX), a well-established anticancer agent, was evaluated thereafter...
February 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28194237/the-impact-of-micronutrient-status-on-health-correlation-network-analysis-to-understand-the-role-of-micronutrients-in-metabolic-inflammatory-processes-regulating-homeostasis-and-phenotypic-flexibility
#6
Tim J van den Broek, Bas H A Kremer, Marisa Marcondes Rezende, Femke P M Hoevenaars, Peter Weber, Ulrich Hoeller, Ben van Ommen, Suzan Wopereis
BACKGROUND: Vitamins and carotenoids are key micronutrients facilitating the maintenance of health, as evidenced by the increased risk of disease with low intake. Optimal phenotypic flexibility, i.e., the ability to respond to a physiological challenge, is an essential indicator of health status. Therefore, health can be measured by applying a challenge test and monitoring the response of relevant phenotypic processes. In this study, we assessed the correlation of three fat-soluble vitamins, (i...
2017: Genes & Nutrition
https://www.readbyqxmd.com/read/28167444/lipophilic-antioxidants-and-lipid-peroxidation-in-yellow-perch-subjected-to-various-anthropogenic-influences-along-the-st-lawrence-river-qc-canada
#7
Catherine Landry, Magali Houde, Philippe Brodeur, Philip Spear, Monique Boily
In Lake Saint-Pierre (LSP), the last great widening of the St. Lawrence River (province of Québec, Canada), the yellow perch has been experiencing a significant decline since the mid-1990s. The combined effect of several stressors (deterioration of habitats appropriate for reproduction and growth, invasive species and poor water quality) seems to exert considerable influence on the yellow perch population in LSP, characterized by low recruitment. To better understand possible stressor effects at the biochemical level, LSP yellow perch were compared with other sites along a gradient of increasing human influences from upstream to downstream along the St...
February 7, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28163080/neuroprotective-effects-of-lycopene-in-spinal-cord-injury-in-rats-via-antioxidative-and-anti-apoptotic-pathway
#8
Wei Hu, Hongbo Wang, Zhenfeng Liu, Yanlu Liu, Rong Wang, Xiao Luo, Yifei Huang
Oxidative damage induced-mitochondrial dysfunction and apoptosis has been widely studied in spinal cord injury (SCI). Lycopene, a polyunsaturated hydrocarbon, has the highest antioxidant capacity compared to the other carotenoids. However, the role of lycopene in SCI is unknown. In the present study, we evaluated the antioxidant effects of lycopene on mitochondrial dysfunction and apoptosis following T10 contusion SCI in rats. The rats were randomized into 5 groups: the sham group, the SCI group and the SCI pre-treated with lycopene (5, 10, or 20mg/kg) group...
February 3, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28161629/gene-expression-analysis-of-enzymes-of-the-carotenoid-biosynthesis-pathway-involved-in-%C3%AE-cryptoxanthin-accumulation-in-wild-raspberry-rubus-palmatus
#9
Kouichi Mizuno, Tetsuo Tokiwano, Yuko Yoshizawa
β-cryptoxanthin (β-Cry), a xanthophyll, is unlike other abundant carotenoids, such as α-carotene, β-carotene, lycopene, lutein, and zeaxanthin. It is not found in most fruits or vegetables but is found only in specific fruits, such as hot chili pepper, persimmon, and citrus fruits. Because recent reports suggest that β-Cry intake is beneficial to human health, the xanthophyll requires further investigation. Although β-Cry accumulates in the fruit of wild raspberry, Rubus palmatus, it is not present in cultivated raspberry...
February 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28149676/genomic-insight-into-pathogenicity-of-dematiaceous-fungus-corynespora-cassiicola
#10
Hong Keat Looi, Yue Fen Toh, Su Mei Yew, Shiang Ling Na, Yung-Chie Tan, Pei-Sin Chong, Jia-Shiun Khoo, Wai-Yan Yee, Kee Peng Ng, Chee Sian Kuan
Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in Malaysia (Hsueh, 2011; Nghia et al., 2008). The isolation of UM 591 from a patient's contact lens indicates the pathogenic potential of this dematiaceous fungus in human. However, the underlying factors that contribute to the opportunistic cross-infection have not been fully studied. We employed genome sequencing and gene homology annotations in attempt to identify these factors in UM 591 using data obtained from publicly available bioinformatics databases...
2017: PeerJ
https://www.readbyqxmd.com/read/28146357/effect-of-pasteurization-on-flavonoids-and-carotenoids-in-citrus-sinensis-l-osbeck-cv-cara-cara-and-bahia-juices
#11
Elisa Brasili, Daniela F Seixas Chaves, Ana Augusta O Xavier, Adriana Z Mercadante, Neuza M A Hassimotto, Franco M Lajolo
Orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. Citrus sinensis Osbeck cv. 'Cara Cara' is a bud mutation originated from 'Washington' navel orange, also known as 'Bahia' navel orange. The ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized Bahia and Cara Cara juices using two LC-MS/MS platforms were investigated. Higher ascorbic acid content was observed in Bahia compared to Cara Cara in both pasteurized and nonpasteurized juices...
February 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28139696/rewiring-carotenoid-biosynthesis-in-plants-using-a-viral-vector
#12
Eszter Majer, Briardo Llorente, Manuel Rodríguez-Concepción, José-Antonio Daròs
Plants can be engineered to sustainably produce compounds of nutritional, industrial or pharmaceutical relevance. This is, however, a challenging task as extensive regulation of biosynthetic pathways often hampers major metabolic changes. Here we describe the use of a viral vector derived from Tobacco etch virus to express a whole heterologous metabolic pathway that produces the health-promoting carotenoid lycopene in tobacco tissues. The pathway consisted in three enzymes from the soil bacteria Pantoea ananatis...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28137214/phytochemical-constituents-ches-and-urease-inhibition-antiproliferative-and-antioxidant-properties-of-elaeagnus-umbellata-thunb
#13
Tevfik Ozen, Semiha Yenigun, Muhammed Altun, Ibrahim Demirtas
Due to the common ethnopharmacological used or scientifically examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellata Thunb. (autumn olive) was worth investigated. In this investigation, we revealed antioxidant, antiproliferative and enzyme inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of E. umbellata as well as contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and b-carotene. The antioxidant activity was screened by phosphomolybdenum assay...
January 27, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28133820/dietary-inclusion-of-tomato-pomace-improves-meat-oxidative-stability-of-young-pigs
#14
C S Correia, C M Alfaia, M S Madeira, P A Lopes, T J S Matos, L F Cunha, J A M Prates, J P B Freire
The aim of this study was to assess the nutritional effect of tomato pomace, in combination with two distinct fat sources (lard or soya bean oil), on longissimus lumborum muscle, subcutaneous fat and liver of young pigs. Forty male pigs of 4 weeks old were randomly assigned to four dietary treatments using two fibre sources (wheat bran or 5.0% tomato pomace) and two fat sources (lard or soya bean oil), in a 2 × 2 factorial design for a 5-week trial. Tomato pomace did not improve colour, fatty acid profile, cholesterol content or lipid oxidation, nor did it yield detectable levels in lycopene or β-carotene in the meat (p > 0...
January 30, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28129549/tomato-and-lycopene-supplementation-and-cardiovascular-risk-factors-a-systematic-review-and-meta-analysis
#15
Ho Ming Cheng, Georgios Koutsidis, John K Lodge, Ammar Ashor, Mario Siervo, José Lara
BACKGROUND AND AIMS: Epidemiological evidence suggests an association between consumption of tomato products or lycopene and lower risk for cardiovascular diseases (CVD). Our aim was to evaluate the state of the evidence from intervention trials on the effect of consuming tomato products and lycopene on markers of cardiovascular (CV) function. We undertook a systematic review and meta-analysis on the effect of supplementing tomato and lycopene on CV risk factors. METHODS: Three databases including Medline, Web of science, and Scopus were searched from inception to August 2016...
January 13, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28126735/integrated-analysis-of-multiomic-data-reveals-the-role-of-the-antioxidant-network-in-the-quality-of-sea-buckthorn-berry
#16
Caiyun He, Guoyun Zhang, Jianguo Zhang, Yanfei Zeng, Juanjuan Liu
Berries of sea buckthorn, known as the "king of vitamin C," are abundant in antioxidants, have attractive colors, and are an excellent material with which to study the relationships between berry color, antioxidants, and berry quality. No study has yet determined the molecular basis of the relationship between sea buckhorn berries and their color and antioxidant levels. By using RNA-seq, liquid chromatography-MS/MS, and liquid chromatography/GC-MS technology and selecting red (darkest colored) and yellow (lightest colored) sea buckthorn berries at different development stages, this study showed that the red and yellow berry resulted from a higher ratio of lycopene to β-carotene and of β-carotene to lycopene content, respectively...
January 26, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28122476/effect-of-increasing-manganese-concentration-in-nutrient-solution-on-the-antioxidant-activity-vitamin-c-lycopene-and-polyphenol-contents-of-tomato-fruit
#17
Małgorzata Muzolf-Panek, Tomasz Kleiber, Anna Kaczmarek
This study evaluated the effect of increasing manganese (Mn) nutrition on the content of antioxidative compounds such as vitamin C, lycopene and polyphenols, and the antioxidant activity of tomato (Lycopersicon esculentum Mill., cvs 'Alboney F1' and 'Emotion F1') fruit. Plants were grown in rockwool using a nutrient solution with the following content of Mn (mg dm(-3)): 0.0, 0.3, 0.6, 1.2, 2.4, 4.8, 9.6 and 19.2. The level of vitamin C and lycopene decreased with the increasing Mn nutrition. Since the colour of fruits was correlated with the change in carotenoid content, the decrease in lycopene content promoted the reduction of redness and increase of yellowness of fruits...
March 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28102015/circulating-antioxidant-levels-and-risk-of-prostate-cancer-by-tmprss2-erg
#18
Rebecca E Graff, Gregory Judson, Thomas U Ahearn, Michelangelo Fiorentino, Massimo Loda, Edward L Giovannucci, Lorelei A Mucci, Andreas Pettersson
BACKGROUND: Few studies have considered etiological differences across molecular subtypes of prostate cancer, despite potential to improve opportunities for precision prevention of a disease for which modifiable risk factors have remained elusive. Factors that lead to DNA double-strand breaks, such as oxidative stress, may promote the formation of the TMPRSS2:ERG gene fusion in prostate cancer. We tested the hypothesis that increasing levels of pre-diagnostic circulating antioxidants, which may reduce oxidative stress, are associated with lower risk of developing TMPRSS2:ERG positive prostate cancer...
January 19, 2017: Prostate
https://www.readbyqxmd.com/read/28096780/molecular-breeding-of-a-novel-orange-brown-tomato-fruit-with-enhanced-beta-carotene-and-chlorophyll-accumulation
#19
Ranjith Kumar Manoharan, Hee-Jeong Jung, Indeok Hwang, Namhee Jeong, Kang Hee Kho, Mi-Young Chung, Ill-Sup Nou
BACKGROUND: Tomatoes provide a significant dietary source of the carotenoids, lycopene and β-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties...
2017: Hereditas
https://www.readbyqxmd.com/read/28094982/bacterial-genome-editing-via-a-designed-toxin-antitoxin-cassette
#20
Jie Wu, Aihua Deng, Qinyun Sun, Hua Bai, Zhaopeng Sun, Xiuling Shang, Yun Zhang, Qian Liu, Yong Liang, Shuwen Liu, Yongsheng Che, Tingyi Wen
Manipulating the bacterial genomes in an efficient manner is essential to biological and biotechnological research. Here, we reprogrammed the bacterial TA systems as the toxin counter-selectable cassette regulated by an antitoxin switch (TCCRAS) for genetic modifications in the extensively studied and utilized Gram-positive bacteria, B. subtilis and Corynebacterium glutamicum. In the five characterized type II TA systems, the RelBE complex can specifically and efficiently regulate cell growth and death by the conditionally controlled antitoxin RelB switch, thereby serving as a novel counter-selectable cassette to establish the TCCRAS system...
January 26, 2017: ACS Synthetic Biology
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