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April M Miguez, Monica P McNerney, Mark P Styczynski
Using cells as microbial factories enables highly specific production of chemicals with many advantages over chemical syntheses. A number of exciting new applications of this approach are in the area of precision metabolic engineering, which focuses on improving the specificity of target production. In recent work, we have used precision metabolic engineering to design lycopene-producing Escherichia coli for use as a low-cost diagnostic biosensor. To increase precursor availability and thus the rate of lycopene production, we heterologously expressed the mevalonate pathway...
2018: Frontiers in Microbiology
Keewan Kim, Enrique F Schisterman, Robert M Silver, Brian D Wilcox, Anne M Lynch, Neil J Perkins, Richard W Browne, Laurie L Lesher, Joseph B Stanford, Aijun Ye, Jean Wactawski-Wende, Sunni L Mumford
Although maternal nutrition may impact fecundity, associations between preconception micronutrients and time to pregnancy (TTP) has not been examined. We assessed the relationship between preconception fat-soluble micronutrient concentrations and TTP among women with 1-2 prior pregnancy losses. This is a prospective cohort study of 1,228 women set in the EAGeR Trial (US, 2007-2011), which assessed the effect of preconception-initiated daily low dose aspirin on reproductive outcomes. We measured preconception zeaxanthin, cryptoxanthin, lycopene, α- and β-carotene, and α- and γ-tocopherol in serum...
May 14, 2018: American Journal of Epidemiology
Nina Kacjan Marsic, Dominik Vodnik, Maja Mikulic-Petkovsek, Robert Veberic, Helena Sircelj
Changes in the photosynthetic traits of plants and metabolic composition of fruits of two tomato cultivars, grafted onto two rootstocks, grown in 3 salinity levels were studied in two growing periods during the season. Increased salinity stress conditions lowered water potential, stomatal conductance and transpiration rate of grafted tomato plants, in both growing period. Water deficit induced stomatal closure, which resulted in stomatal limitation of photosynthesis. The proline content in tomato leaves increased and was closely correlated with salinity...
May 14, 2018: Journal of Agricultural and Food Chemistry
Xindi Li, Yanning Wang, Sha Chen, Huiqin Tian, Daqi Fu, Benzhong Zhu, Yunbo Luo, Hongliang Zhu
Numerous studies have been focusing on breeding tomato plants with enhanced lycopene accumulation, considering its positive effects of fruits on the visual and functional properties. In this study, we used a bidirectional strategy: promoting the biosynthesis of lycopene, while inhibiting the conversion from lycopene to β- and α-carotene. The accumulation of lycopene was promoted by knocking down some genes associated with the carotenoid metabolic pathway. Finally, five genes were selected to be edited in genome by CRISPR/Cas9 system using Agrobacterium tumefaciens -mediated transformation...
2018: Frontiers in Plant Science
İbrahim Ozan Tekeli, Ahmet Ateşşahin, Fatih Sakin, Abdullah Aslan, Songül Çeribaşı, Mustafa Yipel
OBJECTIVE: To compare the potential protective effects of conventional and colon-targeted lycopene (TLC) and linalool (TLN) on acetic acid (AA)-induced ulcerative colitis (UC) in rats. METHODS: Conventional and colon-targeted LC (10 mg/kg) and LN (200 mg/kg) were administered in vivo orally for 7 days and sulfasalazine (100 mg/kg) was also used as reference drug. Then, 4% AA was administered intrarectally to induce UC. Subsequently, the colon tissues were taken as samples for biochemical and histopathological analysis...
May 7, 2018: Inflammopharmacology
Ramesh Kumar Saini, So Hyun Moon, Young-Soo Keum
Globally, the amount of food processing waste has become a major concern for environmental sustainability. The valorization of these waste materials can solve the problems of its disposal. Notably, the tomato pomace and crustacean processing waste presents enormous opportunities for the extraction of commercially vital carotenoids, lycopene, and astaxanthin, which have diverse applications in the food, feed, pharmaceuticals, and cosmetic industries. Moreover, such waste can generate surplus revenue which can significantly improve the economics of food production and processing...
June 2018: Food Research International
Sea C Min, Si Hyeon Roh, Brendan A Niemira, Glenn Boyd, Joseph E Sites, Xuetong Fan, Kimberly Sokorai, Tony Z Jin
Effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Salmonella and the storability of grape tomato were investigated. Grape tomatoes, with or without inoculation with Salmonella, were packaged in a polyethylene terephthalate (PET) commercial clamshell container and cold plasma-treated at 35 kV at 1.1 A for 3 min using a DACP system equipped with a pin-type high-voltage electrode. DACP treatment inactivated Salmonella (p < 0.05) without altering the color or firmness of the grape tomatoes (p > 0...
June 2018: Food Research International
Nguyen Ba Ngoc, Pin Lv, Wen-En Zhao
The molecular mechanisms underlying the chemopreventive effects of carotenoids in different types of cancer are receiving increasing attention. In the present study, the role of peroxisome proliferator-activated receptor γ (PPARγ) in the effect of lycopene and β-carotene on the viability of EC109 human esophageal squamous carcinoma cells was investigated. The viability of EC109 cells was evaluated using MTT assays. The effects of lycopene and β-carotene on the expression of PPARγ, p21WAF1/CIP1 , cyclin D1 and cyclooxygenase-2 (COX-2) were analyzed by western blotting...
May 2018: Oncology Letters
Nenad Stojiljkovic, Sonja Ilic, Vladimir Jakovljevic, Nikola Stojanovic, Slavica Stojnev, Hristina Kocic, Marko Stojanovic, Gordana Kocic
Methotrexate is an antimetabolic drug with a myriad of serious side effects including nephrotoxicity, which presumably occurs due to oxidative tissue damage. Here, we evaluated the potential protective effect of lycopene, a potent antioxidant carotenoid, given in two different pharmaceutical forms in methotrexate-induced kidney damage in rats. Serum biochemical (urea and creatinine) and tissue oxidative damage markers and histopathological kidney changes were evaluated after systemic administration of both lycopene dissolved in corn oil and lycopene encapsulated in nanoliposomes...
2018: Oxidative Medicine and Cellular Longevity
Tanja Groher, Simone Schmittgen, Antje Fiebig, Georg Noga, Mauricio Hunsche
BACKGROUND: We examined the suitability of chlorophyll fluorescence-based indices to monitor and predict concentrations of fruit maturity compounds during tomato ripening under different growing conditions in the greenhouse. The aim of this study was to evaluate the effects of chlorophyll concentration changes on fluorescence-based indices and to exploit the relation between fluorescence and reflectance indices with the corresponding maturity compounds determined analytically. RESULTS: Fruits grown under water deficit matured faster than control fruits as recorded with fluorescence-based indices...
May 2, 2018: Journal of the Science of Food and Agriculture
Preeti Rawat, Anil Kumar, Tryambak Deo Singh, Mahesh Pal
Background: Streblus asper , family Moraceae is well-known important medicinal plant used in the Indian system of medicine. In Ayurveda, stem bark of S. asper is recommended against elephantiasis for which there is still no any other effective medicine in the modern system of medicine. Objectives: In the present work, methanol extract (SAM) and its fractions of S. asper leave tested for in vitro anticancer activity against cancer cell lines (MCF-7, A-549, Hep-G2, and K-562) which claims its folklore importance in cancer and gas chromatography-mass spectrometry identification of extracts was also performed...
April 2018: Pharmacognosy Magazine
Ashay Jain, Gajanand Sharma, Gargi Ghoshal, Prashant Kesharwani, Bhupinder Singh, U S Shivhare, O P Katare
The work entails a novel strategy of formulating the lycopene loaded whey protein isolate nanoparticles (LYC-WPI-NPs) solely using the rational blend of biomacromolecule without using equipment-intensive techniques. The LYC-WPI-NPs were fabricated as a substantial drug delivery platform, with maximum entrapment, spatial and controlled release manners, exceptional plasma concentration, and perspective for discrepancy delivery of therapeutics. Prepared nano-formulations were measured in ultra-fine size (100-350 nm) with sphere-shaped...
April 28, 2018: International Journal of Pharmaceutics
Ahmad Majzoub, Ashok Agarwal
Objective: To explore the current evidence concerning the effect of oral antioxidant supplementation on various male fertility outcomes, as antioxidants are widely available compounds that are commonly used for the treatment of male infertility. Materials and methods: PubMed, Medline and Cochrane electronic databases were searched according to a modified Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guidelines looking for studies investigating the effect of antioxidant therapy on infertile men...
March 2018: Arab Journal of Urology
Hailin Chen, Meijie Li, Changqing Liu, Haibo Zhang, Mo Xian, Huizhou Liu
BACKGROUND: Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. is one of the rate-limiting enzymes in the lycopene biosynthetic pathway and one major target during metabolic engineering. The properties of IDIs differ depending on the sources, but under physiological conditions, IDIs are limited by low enzyme activity, short half-life and weak substrate affinity...
April 30, 2018: Microbial Cell Factories
Ruiping Gao, Fayin Ye, Zhiqiang Lu, Jiajia Wang, Xiao Li Shen, Guohua Zhao
In this paper, the effects and mechanisms of a novel two-step tomato peeling method, hot lye with a post-assistance of ultrasound, were investigated. The present work aims to improve the environmental friendliness of the conventional hot lye tomato peeling method (10% w/v, 97 °C, 45 s). The results showed that 4% (w/v) lye treatment at 97 °C for 30 s with a post-assistance of a 31.97 W/L ultrasound treatment at 70 °C for 50 s achieved a 100% peelability. In this scenario, the peeling yield and lycopene content in the peeled product were significantly higher than the peeling yield and lycopene content with the conventional hot lye peeling method...
July 2018: Ultrasonics Sonochemistry
Essra A Noorwali, Janet E Cade, Victoria J Burley, Laura J Hardie
OBJECTIVES: There is increasing evidence to suggest an association between sleep and diet. The aim of the present study was to examine the association between sleep duration and fruit/vegetable (FV) intakes and their associated biomarkers in UK adults. DESIGN: Cross-sectional. SETTING: Data from The National Diet and Nutrition Survey. PARTICIPANTS: 1612 adults aged 19-65 years were included, pregnant/breastfeeding women were excluded from the analyses...
April 27, 2018: BMJ Open
M Kentson, P Leanderson, P Jacobson, H L Persson
Background : The pathogenesis of chronic obstructive pulmonary disease (COPD) is associated with oxidative stress. Both iron (Fe) and oxygen are involved in the chemical reactions that lead to increased formation of reactive oxygen species. Oxidative reactions are prevented by antioxidants such as carotenoids. Objective : To study the differences in Fe status, carotenoid levels, healthy eating habits, and markers of inflammation and oxidative damage on proteins in subjects with severe COPD ± long-term oxygen therapy (LTOT) and lung-healthy control subjects...
2018: European Clinical Respiratory Journal
Toshiaki Okura, Taisuke Seki, Koji Suzuki, Naoki Ishiguro, Yukiharu Hasegawa
PURPOSE: Oxidative stress is closely associated with the pathogenesis of nontraumatic osteonecrosis of the femoral head (ONFH). This study aimed to determine whether the serum levels of antioxidant nutrients were decreased in patients with ONFH. METHODS: We analyzed the serum levels of antioxidant nutrients in 39 patients with ONFH (ONFH group) and 78 age- and gender-matched healthy people (control group) who voluntarily participated in the Yakumo study, which is a comprehensive health examination program...
May 2018: Journal of Orthopaedic Surgery
Leonardo Ciaccheri, Lorenza Tuccio, Andrea A Mencaglia, Kalina Maja Sikorska-Zimny, Ewelina Hallmann, Artur Kowalski, Anna Grazia Mignani, Stanislaw Kaniszewski, Giovanni Agati
Reflectance spectroscopy represents a useful tool for the non-destructive assessment of tomato lycopene, even in the field. For this reason, a compact, low-cost, LED-based sensor has been developed to measure reflectance in the 400-750 nm spectral range. It was calibrated against wet chemistry and evaluated by Partial Least Squares (PLS) regression analyses. The lycopene prediction models were defined for two open-field cultivated red-tomato varieties: the processing oblong tomatoes of the cv. Calista (average weight: 76 g) and the fresh-consumption round tomatoes of the cv...
April 20, 2018: Journal of Agricultural and Food Chemistry
Aoibheann M McMorrow, Ruth M Connaughton, Tiago R Magalhães, Fiona C McGillicuddy, Maria F Hughes, David Cheishvili, Melissa J Morine, Sean Ennis, Marie-Louise Healy, Edna F Roche, Richard E Tremblay, Moshe Szyf, Fiona E Lithander, Helen M Roche
SCOPE: Chronic inflammation and hypoadiponectinemia are characteristic of obesity-induced insulin resistance (IR). The effect of an anti-inflammatory nutrition supplement (AINS) on IR and adiponectin biology in overweight adolescents was investigated. The secondary objective was to examine the extent to which individuals' biomarker profiles, derived from baseline phenotypes, predicted response or not to the AINS. Additionally, the impact of DNA methylation on intervention efficacy was assessed...
April 17, 2018: Molecular Nutrition & Food Research
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