keyword
MENU ▼
Read by QxMD icon Read
search

peptide microarrays

keyword
https://www.readbyqxmd.com/read/28900133/fcn1-m-ficolin-which-directly-associates-with-immunoglobulin-g1-is-a-molecular-target-of-intravenous-immunoglobulin-therapy-for-kawasaki-disease
#1
Daisuke Okuzaki, Kaori Ota, Shin-Ichi Takatsuki, Yukari Akiyoshi, Kazuyuki Naoi, Norikazu Yabuta, Tsutomu Saji, Hiroshi Nojima
Kawasaki disease (KD), an acute systemic vasculitis of early childhood, is of unknown etiology. High-dose intravenous immunoglobulin (IVIG) is an effective treatment, but its molecular target remains elusive. DNA microarray analysis of peripheral blood mononuclear cells (PBMCs) revealed that at least 21 genes are drastically down-regulated after IVIG treatment in most KD patients. qRT-PCR analysis confirmed that the mRNA levels of five of these genes were considerably reduced in almost all KD patients after IVIG treatment...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28871956/-screening-of-specific-ige-binding-epitopes-of-dust-mite-allergens-using-short-peptide-array
#2
Feixiang Teng, Jinxia Sun, Nan Wang, Lili Yu, Yubao Cui
Objective To screen the possible linear epitopes of major and mid-potency allergens in Dermatophagoides farinae (Der f1, Der f2, Der f4, Der f5 and Der f7). Methods Short peptides were synthesized on the basis of the amino acid sequences in active fraction of Der f1, Der f2, Der f4, Der f5 and Der f7. Each peptide had eight amino acids in length and seven of them were overlapped with each other. Put these peptides to the chip to build microarrays that would have immunoreaction with human serum IgE. Then the chips were scanned to analyze the data...
August 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28867663/high-density-peptide-microarray-exploration-of-the-antibody-response-in-a-rabbit-immunized-with-a-neurotoxic-venom-fraction
#3
Mikael Engmark, Martin C Jespersen, Bruno Lomonte, Ole Lund, Andreas H Laustsen
Polyvalent snakebite antivenoms derive their therapeutic success from the ability of their antibodies to neutralize venom toxins across multiple snake species. This ability results from a production process involving immunization of large mammals with a broad suite of toxins present in venoms. As a result of immunization with this wide range of toxins, many polyvalent antivenoms have a high degree of cross-reactivity to similar toxins in other snake venoms - a cross-reactivity which cannot easily be deconvoluted...
September 1, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28860745/targeting-integrins-with-rgd-conjugated-gold-nanoparticles-in-radiotherapy-decreases-the-invasive-activity-of-breast-cancer-cells
#4
Ping-Hsiu Wu, Yasuhito Onodera, Yuki Ichikawa, Erinn B Rankin, Amato J Giaccia, Yuko Watanabe, Wei Qian, Takayuki Hashimoto, Hiroki Shirato, Jin-Min Nam
Gold nanoparticles (AuNPs) have recently attracted attention as clinical agents for enhancing the effect of radiotherapy in various cancers. Although radiotherapy is a standard treatment for cancers, invasive recurrence and metastasis are significant clinical problems. Several studies have suggested that radiation promotes the invasion of cancer cells by activating molecular mechanisms involving integrin and fibronectin (FN). In this study, polyethylene-glycolylated AuNPs (P-AuNPs) were conjugated with Arg-Gly-Asp (RGD) peptides (RGD/P-AuNPs) to target cancer cells expressing RGD-binding integrins such as α5- and αv-integrins...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28859241/mir-125b-1-and-mir-378a-are-predictive-biomarkers-for-the-efficacy-of-vaccine-treatment-against-colorectal-cancer
#5
Hironori Tanaka, Shoichi Hazama, Michihisa Iida, Ryouichi Tsunedomi, Hiroko Takenouchi, Masao Nakajima, Yukio Tokumitsu, Shinsuke Kanekiyo, Yoshitaro Shindo, Shinobu Tomochika, Yoshihiro Tokuhisa, Kazuhiko Sakamoto, Nobuaki Suzuki, Shigeru Takeda, Shigeru Yamamoto, Shigefumi Yoshino, Tomio Ueno, Yoshihiko Hamamoto, Yusuke Fujita, Hiroaki Tanaka, Ko Tahara, Ryoichi Shimizu, Kiyotaka Okuno, Koji Fujita, Masahiko Kuroda, Yusuke Nakamura, Hiroaki Nagano
Many clinical trials of peptide vaccines have been conducted. However, these vaccines have provided clinical benefits in only a small fraction of patients. The purpose of this study was to explore microRNAs (miRNAs) as novel predictive biomarkers for the efficacy of vaccine treatment against colorectal cancer. First, we performed microarray analysis of pretreatment cancer tissues in a phase I study, in which peptide vaccines alone were administered. Candidate miRNAs were selected by comparison of the better prognosis group with the poorer prognosis group...
August 31, 2017: Cancer Science
https://www.readbyqxmd.com/read/28845576/smu-940-regulates-dextran-dependent-aggregation-and-biofilm-formation-in-streptococcus-mutans
#6
Hidenobu Senpuku, Hideo Yonezawa, Saori Yoneda, Itaru Suzuki, Ryo Nagasawa, Naoki Narisawa
The oral bacterium Streptococcus mutans is the principal agent in the development of dental caries. Biofilm formation by S. mutans requires bacterial attachment, aggregation and glucan formation on the tooth surface under sucrose supplementation conditions. Our previous microarray analysis of clinical strains identified 74 genes in S. mutans that were related to biofilm morphology; however, the roles of almost all these genes in biofilm formation are poorly understood. We investigated the effects of 21 genes randomly selected from our previous study regarding S...
August 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28841770/synthetic-biomaterials-to-rival-nature-s-complexity-a-path-forward-with-combinatorics-high-throughput-discovery-and-high-content-analysis
#7
REVIEW
Douglas Zhang, Junmin Lee, Kristopher A Kilian
Cells in tissue receive a host of soluble and insoluble signals in a context-dependent fashion, where integration of these cues through a complex network of signal transduction cascades will define a particular outcome. Biomaterials scientists and engineers are tasked with designing materials that can at least partially recreate this complex signaling milieu towards new materials for biomedical applications. In this progress report, recent advances in high throughput techniques and high content imaging approaches that are facilitating the discovery of efficacious biomaterials are described...
August 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28821584/transcriptome-analysis-of-mycobacteria-specific-cd4-t-cells-identified-by-activation-induced-expression-of-cd154
#8
Shajo Kunnath-Velayudhan, Michael F Goldberg, Neeraj K Saini, Christopher T Johndrow, Tony W Ng, Alison J Johnson, Jiayong Xu, John Chan, William R Jacobs, Steven A Porcelli
Analysis of Ag-specific CD4(+) T cells in mycobacterial infections at the transcriptome level is informative but technically challenging. Although several methods exist for identifying Ag-specific T cells, including intracellular cytokine staining, cell surface cytokine-capture assays, and staining with peptide:MHC class II multimers, all of these have significant technical constraints that limit their usefulness. Measurement of activation-induced expression of CD154 has been reported to detect live Ag-specific CD4(+) T cells, but this approach remains underexplored and, to our knowledge, has not previously been applied in mycobacteria-infected animals...
August 18, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28813678/therapeutic-antibodies-to-ganglioside-gd2-evolved-from-highly-selective-germline-antibodies
#9
Eric Sterner, Megan L Peach, Marc C Nicklaus, Jeffrey C Gildersleeve
Antibodies play a crucial role in host defense and are indispensable research tools, diagnostics, and therapeutics. Antibody generation involves binding of genomically encoded germline antibodies followed by somatic hypermutation and in vivo selection to obtain antibodies with high affinity and selectivity. Understanding this process is critical for developing monoclonal antibodies, designing effective vaccines, and understanding autoantibody formation. Prior studies have found that antibodies to haptens, peptides, and proteins evolve from polyspecific germline antibodies...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28811026/multiple-epitope-presentation-and-surface-density-control-enabled-by-chemoselective-immobilization-lead-to-enhanced-performance-in-ige-binding-fingerprinting-on-peptide-microarrays
#10
Alessandro Gori, Marina Cretich, Renzo Vanna, Laura Sola, Paola Gagni, Giulia Bruni, Marta Liprino, Furio Gramatica, Samuele Burastero, Marcella Chiari
Multiple ligand presentation is a powerful strategy to enhance the affinity of a probe for its corresponding target. A promising application of this concept lies in the analytical field, where surface immobilized probes interact with their corresponding targets in the context of complex biological samples. Here we investigate the effect of multiple epitope presentation (MEP) in the challenging context of IgE-detection in serum samples using peptide microarrays, and evaluate the influence of probes surface density on the assay results...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28809825/analysis-of-histone-antibody-specificity-with-peptide-microarrays
#11
Evan M Cornett, Bradley M Dickson, Scott B Rothbart
Post-translational modifications (PTMs) on histone proteins are widely studied for their roles in regulating chromatin structure and gene expression. The mass production and distribution of antibodies specific to histone PTMs has greatly facilitated research on these marks. As histone PTM antibodies are key reagents for many chromatin biochemistry applications, rigorous analysis of antibody specificity is necessary for accurate data interpretation and continued progress in the field. This protocol describes an integrated pipeline for the design, fabrication and use of peptide microarrays for profiling the specificity of histone antibodies...
August 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28806738/partial-igf-1-deficiency-is-sufficient-to-reduce-heart-contractibility-angiotensin-ii-sensibility-and-alter-gene-expression-of-structural-and-functional-cardiac-proteins
#12
José Luis González-Guerra, Inma Castilla-Cortazar, Gabriel A Aguirre, Úrsula Muñoz, Irene Martín-Estal, Elena Ávila-Gallego, Miriam Granado, Juan E Puche, Ángel Luis García-Villalón
Circulating levels of IGF-1 may decrease under several circumstances like ageing, metabolic syndrome, and advanced cirrhosis. This reduction is associated with insulin resistance, dyslipidemia, progression to type 2 diabetes, and increased risk for cardiovascular diseases. However, underlying mechanisms between IGF-1 deficiency and cardiovascular disease remain elusive. The specific aim of the present work was to study whether the partial IGF-1 deficiency influences heart and/or coronary circulation, comparing vasoactive factors before and after of ischemia-reperfusion (I/R)...
2017: PloS One
https://www.readbyqxmd.com/read/28793908/transcriptomic-analysis-comparing-mouse-strains-with-extreme-total-lung-capacities-identifies-novel-candidate-genes-for-pulmonary-function
#13
Leema George, Ankita Mitra, Tania A Thimraj, Martin Irmler, Sangeetha Vishweswaraiah, Lars Lunding, Dorothea Hühn, Alicia Madurga, Johannes Beckers, Heinz Fehrenbach, Swapna Upadhyay, Holger Schulz, George D Leikauf, Koustav Ganguly
BACKGROUND: Failure to attain peak lung function by early adulthood is a risk factor for chronic lung diseases. Previously, we reported that C3H/HeJ mice have about twice total lung capacity (TLC) compared to JF1/MsJ mice. We identified seven lung function quantitative trait loci (QTL: Lfnq1-Lfnq7) in backcross/intercross mice derived from these inbred strains. We further demonstrated, superoxide dismutase 3, extracellular (Sod3), Kit oncogene (Kit) and secreted phosphoprotein 1 (Spp1) located on these Lfnqs as lung function determinants...
August 9, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28790847/combined-detection-of-dickkopf-1-subtype-classification-autoantibodies-as-biomarkers-for-the-diagnosis-and-prognosis-of-non-small-cell-lung-cancer
#14
Lei Shen, Xiaoguang Wu, Jinjing Tan, Meng Gu, Yu Teng, Zitong Wang, Wentao Yue
PURPOSE: This study aims to identify the clinical significance of serum autoantibodies against dickkopf-1 (DKK1) and evaluate their feasibility in the immunodiagnosis and prognosis of non-small cell lung cancer (NSCLC). EXPERIMENTAL DESIGN: Epitope mapping by peptide microarray-based serum screening of NSCLC patients (n=72) and healthy controls (n=16) was performed. Indirect ELISA with peptides was used to measure the serum levels of autoantibodies in 206 NSCLC patients and 99 healthy controls...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28763864/-high-expression-of-hnrnpab-kap1-together-promote-poor-prognosis-in-hcc
#15
Z J Zhou, X Y Wang, X Y Xu, Z Q Hu, E B Chen, S L Zhou, W Z Wu, J Zhou, J Fan, Z Dai
Objective: To further understand the interaction protein spectrum of heterogeneous ribonucleoprotein AB (hnRNP AB), and to investigate their clinical significance in hepatocellular carcinoma (HCC). Methods: We carried out mass spectrometry to reveal the specific peptides of KRAB-associated protein 1 (Kap1) and hnRNPAB, and verified their interaction by immunocoprecipitation and western blotting. Expression of hnRNPAB/Kap1 proteins were detected by immunohistochemical staining in the tissue microarrays. Categorical data were analyzed by the chi square test or Fisher exact test; enumeration data between groups were compared using Student t-test or Wilcocon signed rank test; the cumulative recurrence and survival rates were evaluated using the Kaplan-Meier method and the differences were assessed using the log-rank test...
June 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28756193/a-photoclickable-peptide-microarray-platform-for-facile-and-rapid-screening-of-3-d-tissue-microenvironments
#16
Sadhana Sharma, Michael Floren, Yonghui Ding, Kurt R Stenmark, Wei Tan, Stephanie J Bryant
Microarrays are powerful experimental tools for high-throughput screening of cellular behavior in multivariate microenvironments. Here, we present a new, facile and rapid screening method for probing cellular behavior in 3D tissue microenvironments. This method utilizes a photoclickable peptide microarray platform developed using electrospun fibrous poly(ethylene glycol) hydrogels and microarray contact printing. We investigated the utility of this platform with five different peptide motifs and ten cell types including stem, terminally differentiated, cancer or immune cells that were from either primary origin or cell lines and from different species...
July 20, 2017: Biomaterials
https://www.readbyqxmd.com/read/28731125/n-peptide-of-vmip-%C3%A2-reverses-paclitaxel-resistance-by-regulating-mirna-335-in-breast-cancer
#17
Yangyang Wang, Haifeng Wang, Yongxing Ding, Yu Li, Sulian Chen, Lingyu Zhang, Haihua Wu, Jihong Zhou, Kecai Duan, Wenrui Wang, Changjie Chen, Qingling Yang
Acquisition of resistance to paclitaxel is one of the most important problems in treatment of breast cancer patients, but the molecular mechanisms underlying sensitivity to paclitaxel remains elusive. Emerging evidence has demonstrated that microRNAs (miRNAs) play important roles in regulation of cell growth, migration and invasion through inhibiting the expression of its target genes. In our previous studies, we have shown that microRNA-335 (miR‑335) decreased obviously between paclitaxel-resistant (PR) and parental breast cancer cells through miRNA microarray...
July 19, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28714192/secreted-tumor-antigens-immune-biomarkers-for-diagnosis-and-therapy
#18
REVIEW
Els N Meeusen, Elgene Lim, Suresh Mathivanan
With the advent of immunotherapies for cancer, there is growing interest in the identification of tumor antigens. Tumor antigens are self-molecules altered through genetic mutations (neoantigens), protein truncation, protein misfolding or abnormal post translational modifications. To induce an immune response, tumor antigens need to be secreted into the tumor environment and presented to the immune system in the draining lymph nodes, resulting in the generation of tumor-specific effector cells and antibodies...
July 17, 2017: Proteomics
https://www.readbyqxmd.com/read/28708892/cross-recognition-of-a-pit-viper-crotalinae-polyspecific-antivenom-explored-through-high-density-peptide-microarray-epitope-mapping
#19
Mikael Engmark, Bruno Lomonte, José María Gutiérrez, Andreas H Laustsen, Federico De Masi, Mikael R Andersen, Ole Lund
Snakebite antivenom is a 120 years old invention based on polyclonal mixtures of antibodies purified from the blood of hyper-immunized animals. Knowledge on antibody recognition sites (epitopes) on snake venom proteins is limited, but may be used to provide molecular level explanations for antivenom cross-reactivity. In turn, this may help guide antivenom development by elucidating immunological biases in existing antivenoms. In this study, we have identified and characterized linear elements of B-cell epitopes from 870 pit viper venom protein sequences by employing a high-throughput methodology based on custom designed high-density peptide microarrays...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28690651/mutations-of-the-epidermal-growth-factor-receptor-gene-in-triple-negative-breast-cancer
#20
Aeri Kim, Min Hye Jang, Soo Jung Lee, Young Kyung Bae
PURPOSE: Epidermal growth factor receptor (EGFR) is considered a potential therapeutic target for anti-EGFR therapy in triple-negative breast cancer (TNBC). However, the frequency of EGFR gene mutation in TNBC is low and varies with ethnicity. This study aimed to investigate the incidence of EGFR gene mutation in TNBC. METHODS: EGFR protein expression was evaluated by immunohistochemistry in tissue microarrays of 493 TNBC cases using four different primary antibodies, which included mutation-specific antibodies...
June 2017: Journal of Breast Cancer
keyword
keyword
40466
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"