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peptide microarrays

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https://www.readbyqxmd.com/read/28521850/differential-gene-expression-in-porcine-oviduct-during-the-oestrous-cycle
#1
O S Acuña, M Avilés, R López-Úbeda, A Guillén-Martínez, C Soriano-Úbeda, A Torrecillas, P Coy, M J Izquierdo-Rico
The oviduct undergoes changes under the influence of steroid hormones during the oestrous cycle. However, the molecular mechanisms underlying oviductal regulation are not fully understood. The aim of the present study was to identify the gene expression profile of the porcine oviduct in different stages of the cycle using microarray technology. A systematic study was performed on animals at four different stage: prepubertal gilts, and sows in the preovulatory, postovulatory and luteal phase of the oestrous cycle...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28516765/peptide-microarray-based-metal-enhanced-fluorescence-assay-for-multiple-profiling-matrix-metalloproteinases-activities
#2
Zhen Lei, Hua Zhang, Yaoqi Wang, Xianying Meng, Zhenxin Wang
Matrix metalloproteinases (MMPs) are closely associated with cancer cell invasion and metastasis. Herein, a fluorescence resonance energy transfer (FRET)-peptide microarray-based metal enhanced fluorescence (MEF) assay is proposed for multiple and sensitive profiling MMPs activities on a novel Au/Ag@SiO2 substrate. The Au/Ag@SiO2 substrate is prepared by electroless deposition of silver on gold nanoparticle (GNP) seeds, followed by SiO2 shell coating and surface functionalization. The specific FRET peptides are spotted on the Au/Ag@SiO2 substrate to sensitively detect MMPs (MMP-2, 3, 7, 9, 14) via fluorescence recovery by the MMP cleavage of quenched peptide motifs, and further enhanced by MEF...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28511916/pre-treatment-with-amitriptyline-causes-epigenetic-up-regulation-of-neuroprotection-associated-genes-and-has-anti-apoptotic-effects-in-mouse-neuronal-cells
#3
Nguyen Quoc Vuong Tran, An Nghia Nguyen, Kyoko Takabe, Zentaro Yamagata, Kunio Miyake
Antidepressants, such as imipramine and fluoxetine, are known to alter gene expression patterns by inducing changes in the epigenetic status of neuronal cells. There is also some evidence for the anti-apoptotic effect of various groups of antidepressants; however, this effect is complicated and cell-type dependent. Antidepressants of the tricyclic group, in particular amitriptyline, have been suggested to be beneficial in the treatment of neurodegenerative disorders. We examined whether amitriptyline exerts an anti-apoptotic effect via epigenetic mechanisms...
May 13, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/28508359/evolving-relevance-of-neuroproteomics-in-alzheimer-s-disease
#4
Simone Lista, Henrik Zetterberg, Sid E O'Bryant, Kaj Blennow, Harald Hampel
Substantial progress in the understanding of the biology of Alzheimer's disease (AD) has been achieved over the past decades. The early detection and diagnosis of AD and other age-related neurodegenerative diseases, however, remain a challenging scientific frontier. Therefore, the comprehensive discovery (relating to all individual, converging or diverging biochemical disease mechanisms), development, validation, and qualification of standardized biological markers with diagnostic and prognostic functions with a precise performance profile regarding specificity, sensitivity, and positive and negative predictive value are warranted...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28507167/early-activation-of-deleterious-molecular-pathways-in-the-kidney-in-experimental-heart-failure-with-atrial-remodeling
#5
Tomoko Ichiki, Brenda K Huntley, Gail J Harty, S Jeson Sangaralingham, John C Burnett
Heart failure (HF) is a major health problem with worsening outcomes when renal impairment is present. Therapeutics for early phase HF may be effective for cardiorenal protection, however the detailed characteristics of the kidney in early-stage HF (ES-HF), and therefore treatment for potential renal protection, are poorly defined. We sought to determine the gene and protein expression profiles of specific maladaptive pathways of ES-HF in the kidney and heart. Experimental canine ES-HF, characterized by de-novo HF with atrial remodeling but not ventricular fibrosis, was induced by right ventricular pacing for 10 days...
May 2017: Physiological Reports
https://www.readbyqxmd.com/read/28487913/recent-developments-in-protease-activity-assays-and-sensors
#6
REVIEW
Irvine Lian Hao Ong, Kun-Lin Yang
Proteases play a pivotal role in regulating important physiological processes from food digestion to blood clotting. They are also important biomarkers for many diseases such as cancers. The importance of proteases has led to extensive efforts in the screening of proteases and their inhibitors as potential drug molecules. For example, human immunodeficiency virus (HIV) patients have been treated with HIV-1 protease inhibitors to prolong the life expectancy of patients. Such a close relationship between diseases and proteases provides a strong motivation for developing sensitive, selective, and robust protease assays and sensors, which can be exploited to discover new proteases and inhibitors...
May 10, 2017: Analyst
https://www.readbyqxmd.com/read/28475831/microarray-based-immunoassay-with-synthetic-mimotopes-for-the-detection-of-fumonisin-b1
#7
Riikka Peltomaa, Elena Benito-Peña, Rodrigo Barderas, Ursula Sauer, Martin González-Andrade, Maria C Moreno-Bondi
Mimotopes, or epitope mimics, can be applied to competitive immunoassays, for the detection of low molecular weight natural toxicants, as an alternative to toxin-conjugates. In this work, we report the development of a microarray-based immunoassay for the detection of fumonisin B1 using a novel mimotope selected by phage display technology. Fumonisin-specific antibody was used to isolate mimotopes from a 12-mer peptide library in successive selection rounds. Enrichment of antibody binding phages was observed after three panning rounds, and sequence analysis of randomly selected monoclonal phages revealed two conserved peptide sequences...
May 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28468980/identification-of-protective-b-cell-epitopes-within-the-novel-malaria-vaccine-candidate-p-falciparum-schizont-egress-antigen-1
#8
Christina E Nixon, Sangshin Park, Sunthorn Pond-Tor, Dipak Raj, Lynn E Lambert, Sachy Orr-Gonzales, Emma K Barnafo, Jennifer F Friedman, Michal Fried, Patrick E Duffy, Jonathan D Kurtis
Naturally-acquired antibodies to PfSEA-1A are associated with protection against severe malaria in children. Vaccination of mice with PbSEA-1A decreases parasitemia and prolongs survival following Plasmodium berghei ANKA challenge. To enhance the immunogenicity of PfSEA-1A, we identified five linear, B-cell epitopes using peptide microarrays probed with anti-sera from rPfSEA-1A-vaccinated non-human primates. We evaluated the relationship between epitope-specific antibody levels and protection from parasitemia in a longitudinal treatment-reinfection cohort in Western Kenya...
May 3, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28458900/congenital-hyperinsulinism-and-poland-syndrome-in-association-with-10p13-14-duplication
#9
Dinesh Giri, Prashant Patil, Rachel Hart, Mohammed Didi, Senthil Senniappan
SUMMARY: Poland syndrome (PS) is a rare congenital condition, affecting 1 in 30 000 live births worldwide, characterised by a unilateral absence of the sternal head of the pectoralis major and ipsilateral symbrachydactyly occasionally associated with abnormalities of musculoskeletal structures. A baby girl, born at 40 weeks' gestation with birth weight of 3.33 kg (-0.55 SDS) had typical phenotypical features of PS. She had recurrent hypoglycaemic episodes early in life requiring high concentration of glucose and glucagon infusion...
2017: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/28449395/breast-milk-iga-to-foods-has-different-epitope-specificity-than-serum-iga-evidence-for-entero-mammary-link-for-food-specific-iga
#10
Antti E Seppo, Emma M Savilahti, M Cecilia Berin, Hugh A Sampson, Kirsi M Järvinen
BACKGROUND: We have previously shown that maternal cow's milk (CM) elimination results in down-regulation of CM-specific IgA antibody levels in BM, but not in serum, suggesting that an entero-mammary link may exist for food-specific antibody-secreting cells. OBJECTIVE: We sought to investigate whether food-specific IgA epitope profiles differ intra-individually between mother's serum and BM. We also examined how infants' food epitope-specific IgA develops in early infancy and the relationship of IgA epitope recognition with development of cow's milk allergy (CMA)...
April 27, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28445435/microarray-selection-of-cooperative-peptides-for-modulating-enzyme-activities
#11
Jinglin Fu
Recently, peptide microarrays have been used to distinguish proteins, antibodies, viruses, and bacteria based on their binding to random sequence peptides. We reported on the use of peptide arrays to identify enzyme modulators that involve screening an array of 10,000 defined and addressable peptides on a microarray. Primary peptides were first selected to inhibit the enzyme at low μM concentrations. Then, new peptides were found to only bind strongly with the enzyme-inhibitor complex, but not the native enzyme...
April 26, 2017: Microarrays
https://www.readbyqxmd.com/read/28443896/an-msn-peg-ip-drug-delivery-system-and-il13r%C3%AE-2-as-targeted-therapy-for-glioma
#12
Jinlong Shi, Shiqiang Hou, Jianfei Huang, Shanshan Wang, Wei Huan, Chuanjun Huang, Xiaojiang Liu, Rui Jiang, Wenbo Qian, Jingjing Lu, Xiubing Wang, Wei Shi, Rongqin Huang, Jian Chen
A combination of gene therapy and chemotherapy has recently received interest as a targeted therapy for glioma. A mesoporous silica nanoparticle (MSN)-based vehicle coated with IL13Rα2-targeted peptide (IP) using polyethylene glycol (PEG), MSN-PEG-IP (MPI), was constructed and confirmed as a potential glioma-targeted drug delivery system in vitro. In this work, tissue microarray (TMA) results revealed that IL13Rα2 was over-expressed in human glioma tissues and that high expression of IL13Rα2 in patients was associated with poor survival...
April 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28441588/reduced-ena78-levels-as-novel-biomarker-for-major-depressive-disorder-and-venlafaxine-efficiency-result-from-a-prospective-longitudinal-study
#13
Zezhi Li, Zuowei Wang, Chen Zhang, Jun Chen, Yousong Su, Jia Huang, Zhenghui Yi, Chengmei Yuan, Wu Hong, Yong Wang, Zhiguo Wu, Yingyan Hu, Lan Cao, Daihui Peng, Yangtai Guan, Yimin Zou, Shunying Yu, Donghong Cui, Yiru Fang
Although lines of evidence demonstrated that cytokines play an important role in the pathogenesis of major depressive disorder (MDD), none of the them have been established as reliable biomarkers. We use our previous whole-genome cRNA microarray data to identify epithelial cell-derived neutrophil-activating peptide 78 (ENA78), the most differentially expressed cytokine in peripheral blood between MDD patients and healthy controls; and then we confirmed the result by the quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) for mRNA and protein level, respectively, in an independent drug-naïve first-episode sample set...
March 18, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28424716/growth-stimulatory-effects-and-genome-wide-transcriptional-changes-produced-by-protein-hydrolysates-in-maize-seedlings
#14
Chiara Santi, Anita Zamboni, Zeno Varanini, Tiziana Pandolfini
Protein hydrolysates are an emerging class of crop management products utilized for improving nutrient assimilation and mitigating crop stress. They generally consist of a mixture of peptides and free amino acids derived from the hydrolysis of plant or animal sources. The present work was aimed at studying the effects and the action mechanisms of a protein hydrolysate derived from animal residues on maize root growth and physiology in comparison with the effects induced by either free amino acids or inorganic N supply...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28423512/kinase-analysis-of-penile-squamous-cell-carcinoma-on-multiple-platforms-to-identify-potential-therapeutic-targets
#15
Eddy S Yang, Christopher D Willey, Amitkumar Mehta, Michael R Crowley, David K Crossman, Dongquan Chen, Joshua C Anderson, Gurudatta Naik, Deborah L Della Manna, Tiffiny S Cooper, Guru Sonpavde
Penile squamous cell carcinoma (PSCC) is an orphan malignancy with poorly understood biology and suboptimal systemic therapy. Given that kinases may be drivers and readily actionable, we performed comprehensive multiplatform analysis of kinases in PSCC tumor and normal tissue. Fresh frozen tumors were collected from 11 patients with PSCC. After macrodissection to demarcate tumor from normal tissue, the samples underwent multiplatform analysis of kinases. Next Generation Sequencing (NGS) of 517 kinase genes was performed using Agilent Kinome capture and run on the Illumina MiSeq at PE150bp...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418903/kinomic-profiling-identifies-focal-adhesion-kinase-1-as-a-therapeutic-target-in-advanced-clear-cell-renal-cell-carcinoma
#16
Arindam P Ghosh, Christopher D Willey, Joshua C Anderson, Karim Welaya, Dongquan Chen, Amitkumar Mehta, Pooja Ghatalia, Ankit Madan, Gurudatta Naik, Sunil Sudarshan, Guru Sonpavde
The introduction of targeted therapies has caused a paradigm shift in the treatment of metastatic clear cell (cc)-renal cell carcinoma (RCC). We hypothesized that determining differential kinase activity between primary and metastatic tumor sites may identify critical drivers of progression and relevant therapeutic targets in metastatic disease. Kinomic profiling was performed on primary tumor and metastatic tumor deposits utilizing a peptide substrate microarray to detect relative tyrosine phosphorylation activity...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415792/a-cd44-specific-peptide-rp-1-exhibits-capacities-of-assisting-diagnosis-and-predicting-prognosis-of-gastric-cancer
#17
Weiming Li, Huan Jia, Jichang Wang, Hao Guan, Yan Li, Dan Zhang, Yanan Tang, Thomas D Wang, Shaoying Lu
Early diagnosis and evaluation of prognosis are both crucial for preventing poor prognosis of patients with gastric cancer (GC), a leading cause of cancer-related deaths worldwide. Cluster of differentiation 44 (CD44), an indicator of cancer stem cells, can be specifically targeted by molecular probes and detected in tissues of GC in a large quantity. In current study we found that RP-1, a specific peptide binding to CD44 protein, exhibited the potentials of specific binding to CD44 high-expressing cancer cells both in vitro and in vivo, and the capacity of predicting prognosis of human GC in a microarray assay...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414692/neuronal-fibers-and-neurotransmitter-receptor-expression-in-the-human-endolymphatic-sac
#18
Martin Nue Møller, Svend Kirkeby, Jonas Vikeså, Finn Cilius Nielsen, Per Cayé-Thomasen
INTRODUCTION: Recent studies suggest that the human endolymphatic sac (ES) may have multiple functions, including an ion-transport capacity comparable to the kidney, an immunological capacity and a possible natriuretic capacity. Further, there have been speculations of a yet undefined role in intracranial pressure homeostasis. The anatomical location towards the sigmoid sinus would suggest a possible endo- and/or paracrine signaling. However, neuronal connections may also apply, but it remains very scarcely explored in the human ES...
June 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28401067/a-nuclease-from-streptococcus-mutans-facilitates-biofilm-dispersal-and-escape-from-killing-by-neutrophil-extracellular-traps
#19
Jia Liu, Luping Sun, Wei Liu, Lihong Guo, Zhaohui Liu, Xi Wei, Junqi Ling
Streptococcus mutans is the primary etiologic agent of dental caries and occasionally infective endocarditis, with the ability to form biofilms and disperse cells into distal sites to exacerbate and spread infection. In this study, we identified a nuclease (DeoC) as a S. mutans biofilm dispersal modulating factor through microarray analysis. In vitro assays revealed a dispersal defect of a deoC deletion mutant, and functional studies with purified protein were indicative of the biofilm dispersal activity of DeoC...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28381638/antibody-epitopes-identified-in-critical-regions-of-dengue-virus-nonstructural-1-protein-in-mouse-vaccination-and-natural-human-infections
#20
Tomer Hertz, P Robert Beatty, Zachary MacMillen, Sarah S Killingbeck, Chunling Wang, Eva Harris
Dengue is a global public health problem and is caused by four dengue virus (DENV) serotypes (DENV1-4). A major challenge in dengue vaccine development is that cross-reactive anti-DENV Abs can be protective or potentially increase disease via Ab-dependent enhancement. DENV nonstructural protein 1 (NS1) has long been considered a vaccine candidate as it avoids Ab-dependent enhancement. In this study, we evaluated survival to challenge in a lethal DENV vascular leak model in mice immunized with NS1 combined with aluminum and magnesium hydroxide, monophosphoryl lipid A + AddaVax, or Sigma adjuvant system+CpG DNA, compared with mice infected with a sublethal dose of DENV2 and mice immunized with OVA (negative control)...
May 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
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