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Clinical flow cytometry

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https://www.readbyqxmd.com/read/29334191/exogenous-oestrogen-inhibits-genital-transmission-of-cell-associated-hiv-1-in-dmpa-treated-humanized-mice
#1
Nirk E Quispe Calla, Rodolfo D Vicetti Miguel, Melissa E Glick, Jesse J Kwiek, Janelle M Gabriel, Thomas L Cherpes
INTRODUCTION: HIV affects more women than any other life-threatening infectious agent, and most infections are sexually transmitted. HIV must breach the female genital tract mucosal barrier to establish systemic infection, and clinical studies indicate virus more easily evades this barrier in women using depot-medroxyprogesterone acetate (DMPA) and other injectable progestins for contraception. Identifying a potential mechanism for this association, we learned DMPA promotes susceptibility of wild-type mice to genital herpes simplex virus type 2 (HSV-2) infection by reducing genital tissue expression of the cell-cell adhesion molecule desmoglein-1 (DSG-1) and increasing genital mucosal permeability...
January 2018: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/29333098/mir-22-3p-regulates-cell-proliferation-and-inhibits-cell-apoptosis-through-targeting-the-eif4ebp3-gene-in-human-cervical-squamous-carcinoma-cells
#2
Kang-Tai Lv, Zhu Liu, Jie Feng, Wei Zhao, Tao Hao, Wen-Yan Ding, Jing-Ping Chu, Ling-Juan Gao
Background: MicroRNAs (miRNAs) are non-coding small RNAs that function as negative regulators of gene expression and are involved in tumour biology. The eIF4E-binding proteins (eIF4EBPs) play essential roles in preventing translation initiation and inhibiting protein synthesis at a global or message-specific level in a variety of tumours. Methods: According to comparative miRNA profiles of clinical cervical cancer and non-cancerous cervical tissue specimens, several miRNAs were aberrantly expressed in the cervical cancer samples...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29330859/differential-effects-of-phototherapy-adalimumab-and-betamethasone-calcipotriol-on-effector-and-regulatory-t-cells-in-psoriasis
#3
I S Kotb, B J Lewis, R N Barker, A D Ormerod
BACKGROUND: Psoriasis is a chronic T cell-mediated skin disease with marked social and economic burdens. Current treatments are unsatisfactory, with unpredictable remission times and incompletely understood modes of action. Recent advances in our understanding of the pathogenesis of psoriasis identify the imbalance between CD4+ T effector cells, particularly the T helper (Th) 17 subset, and regulatory T cells (Treg) as key to the development of psoriatic lesions, and therefore a novel therapeutic target...
January 12, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29330221/minimal-measurable-residual-disease-in-aml-consensus-document-from-eln-mrd-working-party
#4
Gerrit J Schuurhuis, Michael Heuser, Sylvie Freeman, Marie-Christine Béné, Francesco Buccisano, Jacqueline Cloos, David Grimwade, Torsten Haferlach, Robert K Hills, Christopher S Hourigan, Jeffrey L Jorgensen, Wolfgang Kern, Francis Lacombe, Luca Maurillo, Claude Preudhomme, Bert A van der Reijden, Christian Thiede, Adriano Venditti, Paresh Vyas, Brent L Wood, Roland B Walter, Konstanze Döhner, Gail J Roboz, Gert J Ossenkoppele
Measurable residual disease (MRD, previously termed minimal residual disease) is an independent, post-diagnosis, prognostic indicator in acute myeloid leukemia (AML) that is important for risk stratification and treatment planning, in conjunction with other well-established clinical, cytogenetic, and molecular data assessed at diagnosis. MRD can be evaluated using a variety of multi-parameter flow cytometry (MFC) and molecular protocols but, to date, these approaches have not been qualitatively or quantitatively standardized, making their use in clinical practice challenging...
January 12, 2018: Blood
https://www.readbyqxmd.com/read/29330182/fluorescence-recovery-allows-the-implementation-of-a-fluorescence-reporter-gene-platform-applicable-for-the-detection-and-quantification-of-horizontal-gene-transfer-in-anoxic-environments
#5
Rafael Pinilla, Leise Riber, Søren J Sørensen
The study of horizontal gene transfer (HGT) in microbial communities has been revolutionized by significant advances in cultivation-independent methods based on fluorescence reporter gene technologies. Recently, the combination of these novel approaches with flow cytometry has presented itself as one of the most powerful tools to study the spread of mobile genetic elements (MGEs) in the environment. However, the use of fluorescent markers like green fluorescent protein (GFP) and mCherry is limited by environmental constraints that affect the correct maturation of their fluorophores, such as oxygen availability and pH levels...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29328455/role-of-mir-21-in-the-growth-and-metastasis-of-human-salivary-adenoid-cystic-carcinoma
#6
Fei Yan, Chao Wang, Ting Li, Wenyan Cai, Jinhu Sun
Aberrant microRNA (miRNA/miR) expression has been reported in various cancer types. miR‑21, which is considered to be a proto-oncogene and is frequently overexpressed in certain cancer types, has been implicated in tumorigenesis. The aim of the present study was to investigate the effect of miR‑21 degradation on tumor progression and its potential mechanisms in human salivary adenoid cystic carcinoma (SACC) development. Results of reverse transcription‑quantitative polymerase chain reaction analysis indicated that SACC cells with high metastatic potential (SACC‑LM cells) exhibited a significantly higher expression of miR‑21 compared with SACC cells with a lower metastatic potential (SACC‑83 cells)...
January 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328397/aberrant-expression-of-il-23-il-23r-in-patients-with-breast-cancer-and-its-clinical-significance
#7
Shuhai Sheng, Jinji Zhang, Jianzhong Ai, Xueli Hao, Ruishen Luan
Breast cancer tissues and adjacent tissues were collected from 32 patients who were treated in The Third Hospital of Chengde City. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that, compared with the adjacent tissues, interleukin (IL)‑23/IL‑23 receptor (R) gene expression levels were notably higher in breast cancer tissues. Furthermore, IL‑23 and IL‑23R expression levels were positively correlated with patients' tumor size, TNM stage and metastasis. Recombinant human (rh) IL‑23 (10 ng/ml) was used for the stimulation of the MCF‑7 cell line...
January 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29327288/expression-of-pfkfb3-and-ki67-in-lung-adenocarcinomas-and-targeting-pfkfb3-as-a-therapeutic-strategy
#8
Xiaoli Li, Jian Liu, Li Qian, Honggang Ke, Chan Yao, Wei Tian, Yifei Liu, Jianguo Zhang
Phosphofructokinase-2/fructose-2, 6-bisphosphatase 3 (PFKFB3) catalyzes the synthesis of F2,6BP, which is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1): the rate-limiting enzyme of glycolysis. During tumorigenesis, PFKFB3 increases glycolysis, angiogenesis, and tumor progression. In this study, our aim was to investigate the significance of PFKFB3 and Ki67 in human lung adenocarcinomas and to target PFKFB3 as a therapeutic strategy. In this study, we determined the expression levels of PFKFB3 mRNA and proteins in cancerous and normal lung adenocarcinomas by quantitative reverse transcription PCR (qRT-PCR), Western blot analysis, and tissue microarray immunohistochemistry analysis, respectively...
January 11, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29323734/inhibitory-effect-of-circulating-fibrocytes-on-injury-repair-in-acute-lung-injury-acute-respiratory-distress-syndrome-mice-model
#9
Wenlin Tai, Jinyu Li, Boyang Zheng, Hanxin Wu, Jiawei Ding, Ling Gao, Zhaoxin Dong
The study was aimed to explore the functions of circulating fibrocytes (CFs) on injury repair in acute lung injury/acute respiratory distress syndrome (ALI/ARDS) mice model and its clinical value as a biomarker for ALI/ARDS. ALI/ARDS mice model was established by intratracheal instillation of lipopolysaccharide (LPS). Mononuclear cells were isolated from peripheral blood of ALI/ARDS model and flow cytometry was used to measure CFs defined as cells positive for CD45 and collagen-1. Histological changes of lung tissues were evaluated by H&E staining and Masson's trichrome staining...
January 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29323085/cd19-negative-b-lineage-acute-lymphoblastic-leukemia-a-diagnostic-and-therapeutic-challenge
#10
Shashi Bansal, Upendra Sharma, Akansha Jain, Richa Sharma, Bhargav Yagnik
B-lineage acute lymphoblastic leukemia (B-ALL) is an aggressive neoplasm of B-lymphocyte precursors that express the pan B-cell marker CD19 in all the cases. Rarely, a case may be assigned as B-lineage even if CD19 is negative. Here, a 16-year-old male presented with complaints of pain abdomen, on and off fever, joint pain, and hepatosplenomegaly for 2 months. Bone marrow examination was suggestive of acute leukemia with numerous leukoblasts on aspiration. On flow cytometry, gated blast population was negative for CD19, cytoCD3, and myeloperoxidase MPO and positive for CD34, TdT, HLA-DR, CD22, CD79a, and CD10...
October 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29321718/a-novel-notch1-missense-mutation-c1133y-in-the-abruptex-domain-exhibits-enhanced-proliferation-and-invasion-in-oral-squamous-cell-carcinoma
#11
Yang Zheng, Zhao Wang, Xu Ding, Wei Zhang, Gang Li, Laikui Liu, Heming Wu, Wenyi Gu, Yunong Wu, Xiaomeng Song
Background: Notch1 has been regarded as a fundamental regulator in tissue differentiation and stem cell properties. Recently, Notch1 mutations have been reported intensively both in solid tumors and in hematopoietic malignancies. However, little is known about the biological effect and the clinical implication of these reported mutations. Previously, we discovered several missense mutations in the Notch1 receptor in a Chinese population with oral squamous cell carcinoma (OSCC). Methods: We selected a 'hotspot' mutation in the Abruptex domain (C1133Y)...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29321629/chiloscyphenol-a-derived-from-chinese-liverworts-exerts-fungicidal-action-by-eliciting-both-mitochondrial-dysfunction-and-plasma-membrane-destruction
#12
Sha Zheng, Wenqiang Chang, Ming Zhang, Hongzhuo Shi, Hongxiang Lou
This study aimed to characterize the antifungal effects of chiloscyphenol A (CA), a natural small molecule isolated from Chinese liverworts, and investigate its mode of action. CA was effective against five tested Candida species with a minimal inhibitory concentration (MIC) of 8-32 μg/ml and exhibited fungicidal activity against Candida albicans in both the planktonic state and mature biofilms. The in vivo study using Caenorhabditis elegans showed that CA prolonged the survival of C. albicans infected worms...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29319128/the-preliminary-study-on-expression-and-clinical-value-of-platelet-derived-growth-factor-bb-hypoxia-inducible-factor-1%C3%AE-and-c-c-motif-chemokine-receptor-2-in-the-peripheral-blood-during-the-pathogenesis-of-graves-disease
#13
Ying Liu, Jinglan Tang, Qiaohong Hu, Kefeng Lu, Chunjie Hou
Backgroud: Platelet-derived growth factor BB (PDGF-BB) plays an important role in the development of GD(Graves' disease). However, it is still unknown whether PDGF-BB is expressed in peripheral blood and whether the expression of PDGF-BB contributes to GD. We aim to study the expression of PDGF-BB, Hypoxia inducible factor(HIF)-1α and C-C motif chemokine receptor(CCR)-2 in peripheral blood of patients with GD and explore its effect and potential mechanism in pathogenesis. METHODS: 41 patients with GD (GD group) and 45 healthy people(control group) were chosen...
January 10, 2018: Endokrynologia Polska
https://www.readbyqxmd.com/read/29318644/description-and-prognostic-significance-of-the-kinetics-of-minimal-residual-disease-status-in-adults-with-acute-lymphoblastic-leukemia-treated-with-hypercvad
#14
Ryan D Cassaday, Philip A Stevenson, Brent L Wood, Pamela S Becker, Paul C Hendrie, Brenda M Sandmaier, Jerald L Radich, Andrei R Shustov
HyperCVAD is a commonly-used regimen for adults with newly-diagnosed acute lymphoblastic leukemia (ALL). However, relatively little is known about the application of minimal residual disease (MRD) detection with this treatment. To address this, we studied 142 adults with ALL treated with hyperCVAD over a 10-year period who had MRD assessed by either multi-parameter flow cytometry or (for patients with Philadelphia chromosome positive ALL) reverse transcriptase polymerase chain reaction for the BCR-ABL1 translocation...
January 10, 2018: American Journal of Hematology
https://www.readbyqxmd.com/read/29317687/the-synergistic-bactericidal-effect-of-vancomycin-on-utmd-treated-biofilm-involves-damage-to-bacterial-cells-and-enhancement-of-metabolic-activities
#15
Jian Hu, Ning Zhang, Lifang Li, Ning Zhang, Yanfen Ma, Chedong Zhao, Qian Wu, Ying Li, Nianan He, Xiaoqin Wang
In this study, the synergistic effect of vancomycin, a cell wall synthesis inhibitor, and ultrasound-targeted microbubble destruction (UTMD), on cell viability of Staphylococcus epidermidis, embedded in biofilm, was investigated. Biofilms are the leading causes of antibiotic-resistant bacterial infections of medical implants and prosthetics worldwide. The antibiotic-resistant nature of biofilm-embedded pathogens poses a critical challenge to the medical community. Previously, studies have demonstrated the efficacy of using ultrasound waves and UTMD in circumventing this problem...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29316374/switched-memory-b-cells-are-increased-in-oligoarticular-and-polyarticular-juvenile-idiopathic-arthritis-and-their-change-over-time-is-related-to-response-to-tnf-inhibitors
#16
Emiliano Marasco, Angela Aquilani, Simona Cascioli, Gian Marco Moneta, Ivan Caiello, Chiara Farroni, Ezio Giorda, Valentina D'Oria, Denise Pires Marafon, Silvia Magni-Manzoni, Rita Carsetti, Fabrizio De Benedetti
OBJECTIVES: To investigate if abnormalities in B-cell subsets in JIA patients correlated with clinical features and response to treatment. METHODS: 109 patients diagnosed with oligo-JIA or poly-JIA were enrolled in the study. B-cell subsets in peripheral blood and synovial fluid were analyzed by flow cytometry. RESULTS: Switched memory B-cells (SwM B-cells) were significantly (p<0.0001) increased in patients compared to age- matched healthy controls (HC)...
January 5, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29316248/a-standardized-flow-cytometry-procedure-for-the-monitoring-of-regulatory-t-cells-in-clinical-trials
#17
Pitoiset Fabien, Barbié Michèle, Monneret Guillaume, Braudeau Cécile, Pochard Pierre, Pellegrin Isabelle, Trauet Jacques, Labalette Myriam, Klatzmann David, Rosenzwajg Michelle
BACKGROUND: Quantification of regulatory T cells is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections and allergies. Human regulatory T cells are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29316245/flow-cytometric-aberrancies-in-plasma-cell-myeloma-and-mgus-correlation-with-laboratory-parameters
#18
Sarika Gupta, Nitin J Karandikar, Timothy Ginader, Andrew M Bellizzi, Carol J Holman
BACKGROUND: Multiparametric flow cytometry (MFC) is a useful tool for diagnosis of plasma cell dyscrasias and assessment of minimal residual disease (MRD) in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal plasma cells (PCs) of plasma cell myeloma (PCM) and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated. METHODS: We retrospectively identified all newly diagnosed treatment-naive PCM and MGUS patients between 09/2014 and 06/2015 who underwent 10-color flow-cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29315585/ndrg2-deficiency-ameliorates-neurodegeneration-in-experimental-autoimmune-encephalomyelitis
#19
Thuong Manh Le, Mika Takarada-Iemata, Hieu Minh Ta, Jureepon Roboon, Hiroshi Ishii, Takashi Tamatani, Yasuko Kitao, Tsuyoshi Hattori, Osamu Hori
N-myc downstream-regulated gene 2 (NDRG2) is a differentiation- and stress-associated molecule that is predominantly expressed in astrocytes in the central nervous system. In this study, we examined the expression and role of NDRG2 in experimental autoimmune encephalomyelitis (EAE), which is an animal model of multiple sclerosis. Western blot and immunohistochemical analysis revealed that the expression of NDRG2 was observed in astrocytes of spinal cord, and was enhanced after EAE induction. A comparative analysis of wild-type (WT) and Ndrg2-/- mice revealed that deletion of Ndrg2 ameliorated the clinical symptoms of EAE...
January 6, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29315012/development-of-an-anti-her2-monoclonal-antibody-h2mab-139-against-colon-cancer
#20
Mika K Kaneko, Shinji Yamada, Shunsuke Itai, Yukinari Kato
Human epidermal growth factor receptor 2 (HER2) expression has been reported in several cancers, such as breast, gastric, lung, pancreatic, and colorectal cancers. HER2 is overexpressed in those cancers and is associated with poor clinical outcomes. Trastuzumab, a humanized anti-HER2 antibody, provides significant survival benefits for patients with HER2-overexpressing breast cancers and gastric cancers. In this study, we developed a novel anti-HER2 monoclonal antibody (mAb), H2Mab-139 (IgG1, kappa) and investigated it against colon cancers using flow cytometry, western blot, and immunohistochemical analyses...
January 9, 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
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